@article{jennette_rufty_macfall_2003, title={Magnetic resonance image visualization of plant roots in situ: A tool for characterizing root morphology}, ISBN={0891181520}, DOI={10.2134/asaspecpub66.c6}, abstractNote={Magnetic resonance imaging (MRI) is a technique that holds promise for the noninvasive study of root systems. The purpose of this chapter is to provide a brief overview of the MRI technique and evaluate new applications for MRI visualization of a crop plant—soybean. Soybean seedlings were grown for 8 days in sand, at optimum fertility and pH. Root systems were imaged in situ, without removal from soil, in a General Electric Signa MRI unit customized for high-resolution research studies. Three-dimensional renderings were produced using the software program VOXELVIEW. The images allowed relatively clear allowed visualization of primary, basal, and lateral roots, and calculations of root system volume were similar to those obtained from water displacement and water content estimates.}, journal={Digital imaging and spectral techniques : applications to precision agriculture and crop physiology : proceedings of a Symposium sponsored by Division C-2 of the Crop Science Society of America, the USDA-ARS, and the Rockfeller Foundation in Minneapolis, MN, November 2001}, publisher={Madison, Wis. : American Society of Agronomy}, author={Jennette, M. W. and Rufty, T. W. and MacFall, J. S.}, year={2003} } @article{villagarcia_carter_rufty_niewoehner_jennette_arrellano_2001, title={Genotypic rankings for aluminum tolerance of soybean roots grown in hydroponics and sand culture}, volume={41}, ISSN={["1435-0653"]}, DOI={10.2135/cropsci2001.4151499x}, abstractNote={Screening methodology remains a practical barrier in the breeding of Al‐tolerant soybean [Glycine max (L.) Merr.]. Our objectives were to (i) develop a repeatable sand‐media culture method for Al tolerance screening of plants, (ii) compare Al response of genotypes in sand culture to a standard hydroponics‐based seedling culture, and (iii) establish a practical guide for the use of hydroponics and sand‐culture screening methods in the selection of Al‐tolerant soybean. We developed a sand‐media culture method and imposed 0 and 450 μM Al3+ activity treatments upon 10 diverse soybean genotypes. The experiment employed a randomized complete block design with nine replications. Root weight and relative root surface area (RRSA) were determined at 18 d after transplanting (DAT). In hydroponics, the genotypes were compared for taproot elongation after 3 d of exposure to 0, 2, and 5 μM Al3+ activity treatments in a split plot design with six replications. Aluminum stress was imposed successfully (approximately 57% of the growth in control) in hydroponics and sand culture, but discrepancies between methods were apparent. The hydroponics‐based seedling screen produced an inflated range of genotypic response and altered Al tolerance rankings in comparison with sand culture. ‘Perry’, which was tolerant to Al in sand culture, was remarkably sensitive to Al in hydroponics. Despite the discrepancies, seedling‐based screening successfully identified three (PI 417021, PI 416937, and Biloxi) of the four genotypes that were most tolerant to Al in sand culture. Results suggested that seedling screens can play a practical role in breeding. However, their application to a specific breeding population should be validated with older plants and solid media. The RRSA appeared to be a promising measure of A1 tolerance for soybean roots.}, number={5}, journal={CROP SCIENCE}, author={Villagarcia, MR and Carter, TE and Rufty, TW and Niewoehner, AS and Jennette, MW and Arrellano, C}, year={2001}, pages={1499–1507} } @article{wright_jennette_coble_rufty_1999, title={Root morphology of young Glycine max, Senna obtusifolia, and Amaranthus palmeri}, volume={47}, number={6}, journal={Weed Science}, author={Wright, S. R. and Jennette, M. W. and Coble, H. D. and Rufty, T. W.}, year={1999}, pages={706–711} }