@article{pezzini_taylor_reisig_fritz_2024, title={Cross- pollination in seed- blended refuge and selection for Vip3A resistance in a lepidopteran pest as detected by genomic monitoring}, volume={121}, ISSN={["1091-6490"]}, url={https://doi.org/10.1073/pnas.2319838121}, DOI={10.1073/pnas.2319838121}, abstractNote={
            The evolution of pest resistance to management tools reduces productivity and results in economic losses in agricultural systems. To slow its emergence and spread, monitoring and prevention practices are implemented in resistance management programs. Recent work suggests that genomic approaches can identify signs of emerging resistance to aid in resistance management. Here, we empirically examined the sensitivity of genomic monitoring for resistance management in transgenic Bt crops, a globally important agricultural innovation. Whole genome resequencing of wild North American
            Helicoverpa zea
            collected from non-expressing refuge and plants expressing Cry1Ab confirmed that resistance-associated signatures of selection were detectable after a single generation of exposure. Upon demonstrating its sensitivity, we applied genomic monitoring to wild
            H. zea
            that survived Vip3A exposure resulting from cross-pollination of refuge plants in seed-blended plots. Refuge seed interplanted with transgenic seed exposed
            H. zea
            to sublethal doses of Vip3A protein in corn ears and was associated with allele frequency divergence across the genome. Some of the greatest allele frequency divergence occurred in genomic regions adjacent to a previously described candidate gene for Vip3A resistance. Our work highlights the power of genomic monitoring to sensitively detect heritable changes associated with field exposure to Bt toxins and suggests that seed-blended refuge will likely hasten the evolution of resistance to Vip3A in lepidopteran pests.
          }, number={13}, journal={PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA}, author={Pezzini, Daniela and Taylor, Katherine L. and Reisig, Dominic D. and Fritz, Megan L.}, year={2024}, month={Mar} }
 @article{taylor_hamby_deyonke_gould_fritz_2021, title={Genome evolution in an agricultural pest following adoption of transgenic crops}, volume={118}, ISSN={["1091-6490"]}, DOI={10.1073/pnas.2020853118}, abstractNote={SignificanceEvolution of resistance to management approaches in agricultural landscapes is common and results in economic losses. Early detection of pest resistance prior to significant crop damage would benefit the agricultural community. It has been hypothesized that new genomic approaches could track molecular signals of emerging resistance and trigger efforts to preempt widespread damage. We tested this hypothesis by quantifying genomic changes in the pestHelicoverpa zeaover a 15-y period concurrent with commercialization of transgenicBacillus thuringiensis–expressing crops and their subsequent loss of efficacy. Our results demonstrate the complex nature of evolution in agricultural ecosystems and provide insight into the potential and pitfalls of using genomic approaches for resistance monitoring.}, number={52}, journal={PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA}, author={Taylor, Katherine L. and Hamby, Kelly A. and DeYonke, Alexandra M. and Gould, Fred and Fritz, Megan L.}, year={2021}, month={Dec} }
 @article{fritz_paa_baltzegar_gould_2016, title={Application of a dense genetic map for assessment of genomic responses to selection and inbreeding in Heliothis virescens}, volume={25}, ISSN={["1365-2583"]}, DOI={10.1111/imb.12234}, abstractNote={AbstractAdaptation of pest species to laboratory conditions and selection for resistance to toxins in the laboratory are expected to cause inbreeding and genetic bottlenecks that reduce genetic variation. Heliothis virescens, a major cotton pest, has been colonized in the laboratory many times, and a few laboratory colonies have been selected for Bacillus thuringiensis (Bt) resistance. We developed 350‐bp double‐digest restriction‐site associated DNA‐sequencing (ddRAD‐seq) molecular markers to examine and compare changes in genetic variation associated with laboratory adaptation, artificial selection and inbreeding in this nonmodel insect species. We found that allelic and nucleotide diversity declined dramatically in laboratory‐reared H. virescens as compared with field‐collected populations. The declines were primarily a result of the loss of low frequency alleles present in field‐collected H. virescens. A further, albeit modest decline in genetic diversity was observed in a Bt‐selected population. The greatest decline was seen in H. virescens that were sib‐mated for 10 generations, in which more than 80% of loci were fixed for a single allele. To determine which regions of the genome were resistant to fixation in our sib‐mated line, we generated a dense intraspecific linkage map containing three PCR‐based and 659 ddRAD‐seq markers. Markers that retained polymorphism were observed in small clusters spread over multiple linkage groups, but this clustering was not statistically significant. Overall, we have confirmed and extended the general expectations for reduced genetic diversity in laboratory colonies, provided tools for further genomic analyses and produced highly homozygous genomic DNA for future whole genome sequencing of H. virescens.}, number={4}, journal={INSECT MOLECULAR BIOLOGY}, author={Fritz, M. L. and Paa, S. and Baltzegar, J. and Gould, F.}, year={2016}, month={Aug}, pages={385–400} }
 @article{fritz_walker_miller_severson_dworkin_2015, title={Divergent host preferences of above- and below-ground Culex pipiens mosquitoes and their hybrid offspring}, volume={29}, number={2}, journal={Medical and Veterinary Entomology}, author={Fritz, M. L. and Walker, E. D. and Miller, J. R. and Severson, D. W. and Dworkin, I.}, year={2015}, pages={115–123} }