@article{hickin_kakumanu_schal_2022, title={Effects of Wolbachia elimination and B-vitamin supplementation on bed bug development and reproduction}, volume={12}, ISSN={["2045-2322"]}, url={https://doi.org/10.1038/s41598-022-14505-2}, DOI={10.1038/s41598-022-14505-2}, abstractNote={Abstract}, number={1}, journal={SCIENTIFIC REPORTS}, author={Hickin, Mauri L. and Kakumanu, Madhavi L. and Schal, Coby}, year={2022}, month={Jun} } @article{gonzalez-morales_devries_santangelo_kakumanu_schal_2022, title={Multiple Mechanisms Confer Fipronil Resistance in the German Cockroach: Enhanced Detoxification and Rdl Mutation}, volume={8}, ISSN={["1938-2928"]}, url={https://doi.org/10.1093/jme/tjac100}, DOI={10.1093/jme/tjac100}, abstractNote={Abstract}, journal={JOURNAL OF MEDICAL ENTOMOLOGY}, author={Gonzalez-Morales, Maria A. and DeVries, Zachary C. and Santangelo, Richard G. and Kakumanu, Madhavi L. and Schal, Coby}, editor={Booth, WarrenEditor}, year={2022}, month={Aug} } @article{kakumanu_marayati_schal_apperson_wasserberg_ponnusamy_2021, title={Oviposition-Site Selection of Phlebotomus papatasi (Diptera: Psychodidae) Sand Flies: Attraction to Bacterial Isolates From an Attractive Rearing Medium}, volume={58}, ISSN={["1938-2928"]}, url={https://doi.org/10.1093/jme/tjaa198}, DOI={10.1093/jme/tjaa198}, abstractNote={Abstract}, number={2}, journal={JOURNAL OF MEDICAL ENTOMOLOGY}, publisher={Oxford University Press (OUP)}, author={Kakumanu, Madhavi L. and Marayati, Bahjat F. and Schal, Coby and Apperson, Charles S. and Wasserberg, Gideon and Ponnusamy, Loganathan}, editor={Johnson, RichardEditor}, year={2021}, month={Mar}, pages={518–527} } @article{gonzalez-morales_devries_sierras_santangelo_kakumanu_schal_2021, title={Resistance to Fipronil in the Common Bed Bug (Hemiptera: Cimicidae)}, volume={58}, ISSN={["1938-2928"]}, url={https://doi.org/10.1093/jme/tjab040}, DOI={10.1093/jme/tjab040}, abstractNote={Abstract}, number={4}, journal={JOURNAL OF MEDICAL ENTOMOLOGY}, publisher={Oxford University Press (OUP)}, author={Gonzalez-Morales, Maria A. and DeVries, Zachary and Sierras, Angela and Santangelo, Richard G. and Kakumanu, Madhavi L. and Schal, Coby}, editor={Hribar, LawrenceEditor}, year={2021}, month={Jul}, pages={1798–1807} } @article{kakumanu_marayati_wada-katsumata_wasserberg_schal_apperson_ponnusamy_2021, title={Sphingobacterium phlebotomi sp. nov., a new member of family Sphingobacteriaceae isolated from sand fly rearing substrate}, volume={71}, ISSN={["1466-5034"]}, url={https://doi.org/10.1099/ijsem.0.004809}, DOI={10.1099/ijsem.0.004809}, abstractNote={A Gram-stain-negative, rod-shaped, non-motile, non-spore-forming, aerobic bacterium, designated type strain SSI9T, was isolated from sand fly (Phlebotomus papatasi Scopoli; Diptera: Psychodidae) rearing substrate and subjected to polyphasic taxonomic analysis. Strain SSI9T contained phosphatidylethanolamine as a major polar lipid, MK-7 as the predominant quinone, and C16 : 1ω6c/C16 : 1ω7c, iso-C15 : 0, iso-C17 : 0 3-OH and C16 : 0 as the major cellular fatty acids. Phylogenetic analysis based on 16S rRNA gene sequences revealed that SSI9T represents a member of the genus Sphingobacterium , of the family Sphingobacteriaceae sharing 96.5–88.0 % sequence similarity with other species of the genus Sphingobacterium . The results of multilocus sequence analysis using the concatenated sequences of the housekeeping genes recA, rplC and groL indicated that SSI9T formed a separate branch in the genus Sphingobacterium . The genome of SSI9T is 5 197 142 bp with a DNA G+C content of 41.8 mol% and encodes 4395 predicted coding sequences, 49 tRNAs, and three complete rRNAs and two partial rRNAs. SSI9T could be distinguished from other species of the genus Sphingobacterium with validly published names by several phenotypic, chemotaxonomic and genomic characteristics. On the basis of the results of this polyphasic taxonomic analysis, the bacterial isolate represents a novel species within the genus Sphingobacterium , for which the name Sphingobacterium phlebotomi sp. nov. is proposed. The type strain is SSI9T (=ATCC TSD-210T=LMG 31664T=NRRL B-65603T).}, number={5}, journal={INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY}, author={Kakumanu, Madhavi L. and Marayati, Bahjat Fadi and Wada-Katsumata, Ayako and Wasserberg, Gideon and Schal, Coby and Apperson, Charles S. and Ponnusamy, Loganathan}, year={2021} } @article{travanty_ponnusamy_kakumanu_nicholson_apperson_2019, title={Diversity and structure of the bacterial microbiome of the American dog tick, Dermacentor variabilis, is dominated by the endosymbiont Francisella}, volume={79}, ISSN={["1878-7665"]}, url={https://doi.org/10.1007/s13199-019-00642-2}, DOI={10.1007/s13199-019-00642-2}, number={3}, journal={SYMBIOSIS}, publisher={Springer Science and Business Media LLC}, author={Travanty, Nicholas V. and Ponnusamy, Loganathan and Kakumanu, Madhavi L. and Nicholson, William L. and Apperson, Charles S.}, year={2019}, month={Nov}, pages={239–250} } @article{kakumanu_ma_williams_2019, title={Drought-induced soil microbial amino acid and polysaccharide change and their implications for C-N cycles in a climate change world}, volume={9}, ISSN={["2045-2322"]}, DOI={10.1038/s41598-019-46984-1}, abstractNote={Abstract}, journal={SCIENTIFIC REPORTS}, author={Kakumanu, Madhavi L. and Ma, Li and Williams, Mark A.}, year={2019}, month={Jul} } @article{devries_santangelo_crissman_suazo_kakumanu_schal_2019, title={Pervasive Resistance to Pyrethroids in German Cockroaches (Blattodea: Ectobiidae) Related to Lack of Efficacy of Total Release Foggers}, volume={112}, ISSN={0022-0493 1938-291X}, url={http://dx.doi.org/10.1093/jee/toz120}, DOI={10.1093/jee/toz120}, abstractNote={Abstract}, number={5}, journal={Journal of Economic Entomology}, publisher={Oxford University Press (OUP)}, author={DeVries, Zachary C and Santangelo, Richard G and Crissman, Jonathan and Suazo, Alonso and Kakumanu, Madhavi L and Schal, Coby}, editor={Rust, MichaelEditor}, year={2019}, month={May}, pages={2295–2301} } @article{kakumanu_maritz_carlton_schal_2018, title={Overlapping Community Compositions of Gut and Fecal Microbiomes in Lab-Reared and Field-Collected German Cockroaches}, volume={84}, ISSN={0099-2240 1098-5336}, url={http://dx.doi.org/10.1128/AEM.01037-18}, DOI={10.1128/AEM.01037-18}, abstractNote={The German cockroach infests diverse human-built structures, including homes and hospitals. It produces potent allergens that trigger asthma and disseminates opportunistic pathogens in its feces. A comprehensive understanding of gut and fecal microbial communities of cockroaches is essential not only to understand their contribution to the biology of the cockroach, but also for exploring their clinical relevance. In this study, we compare the diversity of bacteria and eukaryotes in the cockroach gut and feces and assess the variation in the gut microbiota across cockroach populations.}, number={17}, journal={Applied and Environmental Microbiology}, publisher={American Society for Microbiology}, author={Kakumanu, Madhavi L. and Maritz, Julia M. and Carlton, Jane M. and Schal, Coby}, editor={Stabb, Eric V.Editor}, year={2018}, month={Jun} } @article{kakumanu_ponnusamy_sutton_meshnick_nicholson_apperson_2018, title={Prevalence of Rickettsia Species (Rickettsiales: Rickettsiaceae) in Dermacentor variabilis Ticks (Acari: Ixodidae) in North Carolina}, volume={55}, ISSN={["1938-2928"]}, url={http://dx.doi.org/10.1093/jme/tjy074}, DOI={10.1093/jme/tjy074}, abstractNote={Abstract The American dog tick, Dermacentor variabilis (Say), is a vector of spotted fever group (SFG) rickettsiae, including Rickettsia rickettsii the causative organism of Rocky Mountain spotted fever (RMSF). In North Carolina, SFG rickettsioses (including RMSF) are a leading cause of tick-borne illness. Knowledge of the infection rate and geographic distribution of D. variabilis ticks infected with Rickettsia spp. provides information on the spatial distribution of public health risk. Accordingly, we extracted genomic DNA from adult D. variabilis collected from field habitats in 32 North Carolina counties from 2009 to 2013. A nested PCR assay of the 23S-5S intergenic spacer (IGS) region of Rickettsia coupled with reverse line blot hybridization (RLBH) with species-specific probes was used to detect and identify rickettsiae to species. Approximately half of the 532 tick DNA samples exhibited a band of the expected size on agarose gels, indicating infection with Rickettsia spp. RLBH analyses showed R. amblyommatis (formerly ‘Candidatus R. amblyommii’), R. parkeri, and R. montanensis were predominant, while other Rickettsia species detected included R. conorii-like, R. massiliae, R. rhipicephali, R. canadensis, R. bellii, and some unknown Rickettsia spp. Some ticks were infected with more than one Rickettsia species. Notably, several Rickettsia-positive ticks harbored R. rickettsii. DNA sequencing was performed on a portion of the 23S-5S IGS amplicons and the results were concordant with RLB assay results. We conclude that Rickettsia spp. are common in D. variabilis in North Carolina. Geographic patterns in the occurrence of Rickettsia-infected D. variabilis ticks across the counties sampled are discussed.}, number={5}, journal={JOURNAL OF MEDICAL ENTOMOLOGY}, author={Kakumanu, Madhavi L. and Ponnusamy, Loganathan and Sutton, Haley and Meshnick, Steven R. and Nicholson, William L. and Apperson, Charles S.}, year={2018}, month={Sep}, pages={1284–1291} } @article{levine_apperson_levin_kelly_kakumanu_ponnusamy_sutton_salger_caldwell_szempruch_2017, title={Stable Transmission of Borrelia burgdorferi Sensu Stricto on the Outer Banks of North Carolina}, volume={64}, ISSN={1863-1959}, url={http://dx.doi.org/10.1111/zph.12302}, DOI={10.1111/zph.12302}, abstractNote={Summary}, number={5}, journal={Zoonoses and Public Health}, publisher={Wiley}, author={Levine, J. F. and Apperson, C. S. and Levin, M. and Kelly, T. R. and Kakumanu, M. L. and Ponnusamy, L. and Sutton, H. and Salger, S. A. and Caldwell, J. M. and Szempruch, A. J.}, year={2017}, month={Aug}, pages={337–354} } @article{kakumanu_ponnusamy_sutton_meshnick_nicholson_apperson_2016, title={Development and Validation of an Improved PCR Method Using the 23S-5S Intergenic Spacer for Detection of Rickettsiae in Dermacentor variabilis Ticks and Tissue Samples from Humans and Laboratory Animals}, volume={54}, ISSN={["1098-660X"]}, url={http://dx.doi.org/10.1128/jcm.02605-15}, DOI={10.1128/jcm.02605-15}, abstractNote={ABSTRACT}, number={4}, journal={JOURNAL OF CLINICAL MICROBIOLOGY}, author={Kakumanu, Madhavi L. and Ponnusamy, Loganathan and Sutton, Haley T. and Meshnick, Steven R. and Nicholson, William L. and Apperson, Charles S.}, year={2016}, month={Apr}, pages={972–979} } @article{wallace_nicholson_perniciaro_vaughn_funkhouser_juliano_lee_kakumanu_ponnusamy_apperson_et al._2016, title={Incident Tick-Borne Infections in a Cohort of North Carolina Outdoor Workers}, volume={16}, ISSN={["1557-7759"]}, url={http://dx.doi.org/10.1089/vbz.2015.1887}, DOI={10.1089/vbz.2015.1887}, abstractNote={Tick-borne diseases cause substantial morbidity throughout the United States, and North Carolina has a high incidence of spotted fever rickettsioses and ehrlichiosis, with sporadic cases of Lyme disease. The occupational risk of tick-borne infections among outdoor workers is high, particularly those working on publicly managed lands. This study identified incident tick-borne infections and examined seroconversion risk factors among a cohort of North Carolina outdoor workers. Workers from the North Carolina State Divisions of Forestry, Parks and Recreation, and Wildlife (n = 159) were followed for 2 years in a randomized controlled trial evaluating the effectiveness of long-lasting permethrin-impregnated clothing. Antibody titers against Rickettsia parkeri, Rickettsia rickettsii, "Rickettsia amblyommii," and Ehrlichia chaffeensis were measured at baseline (n = 130), after 1 year (n = 82), and after 2 years (n = 73). Titers against Borrelia burgdorferi were measured at baseline and after 2 years (n = 90). Baseline seroprevalence, defined as indirect immunofluorescence antibody titers of 1/128 or greater, was R. parkeri (24%), R. rickettsii (19%), "R. amblyommii" (12%), and E. chaffeensis (4%). Incident infection was defined as a fourfold increase in titer over a 1-year period. There were 40 total seroconversions to at least one pathogen, including R. parkeri (n = 19), "R. amblyommii" (n = 14), R. rickettsii (n = 9), and E. chaffeensis (n = 8). There were no subjects whose sera were reactive to B. burgdorferi C6 antigen. Thirty-eight of the 40 incident infections were subclinical. The overall risk of infection by any pathogen during the study period was 0.26, and the risk among the NC Division of Forest Resources workers was 1.73 times that of workers in other divisions (95% confidence interval [CI]: 1.02, 2.92). The risk of infection was lower in subjects wearing permethrin-impregnated clothing, but not significantly (risk ratio = 0.81; 95% CI: 0.47, 1.39). In summary, outdoor workers in North Carolina are at high risk of incident tick-borne infections, most of which appear to be asymptomatic.}, number={5}, journal={VECTOR-BORNE AND ZOONOTIC DISEASES}, author={Wallace, John W. and Nicholson, William L. and Perniciaro, Jamie L. and Vaughn, Meagan F. and Funkhouser, Sheana and Juliano, Jonathan J. and Lee, Sangmi and Kakumanu, Madhavi L. and Ponnusamy, Loganathan and Apperson, Charles S. and et al.}, year={2016}, month={May}, pages={302–308} } @article{lee_kakumanu_ponnusamy_vaughn_funkhouser_thornton_meshnick_apperson_2014, title={Prevalence of Rickettsiales in ticks removed from the skin of outdoor workers in North Carolina}, volume={7}, ISSN={["1756-3305"]}, url={http://dx.doi.org/10.1186/s13071-014-0607-2}, DOI={10.1186/s13071-014-0607-2}, abstractNote={Tick-transmitted rickettsial diseases, such as ehrlichiosis and spotted fever rickettsiosis, are significant sources of morbidity and mortality in the southern United States. Because of their exposure in tick-infested woodlands, outdoor workers experience an increased risk of infection with tick-borne pathogens. As part of a double blind randomized-controlled field trial of the effectiveness of permethrin-treated clothing in preventing tick bites, we identified tick species removed from the skin of outdoor workers in North Carolina and tested the ticks for Rickettsiales pathogens.Ticks submitted by study participants from April-September 2011 and 2012 were identified to species and life stage, and preliminarily screened for the genus Rickettsia by nested PCR targeting the 17-kDa protein gene. Rickettsia were further identified to species by PCR amplification of 23S-5S intergenic spacer (IGS) fragments combined with reverse line blot hybridization with species-specific probes and through cloning and nucleotide sequence analysis of 23S-5S amplicons. Ticks were examined for Ehrlichia and Anaplasma by nested PCR directed at the gltA, antigen-expressing gene containing a variable number of tandem repeats, 16S rRNA, and groESL genes.The lone star tick (Amblyomma americanum) accounted for 95.0 and 92.9% of ticks submitted in 2011 (n = 423) and 2012 (n = 451), respectively. Specimens of American dog tick (Dermacentor variabilis), Gulf Coast tick (Amblyomma maculatum) and black-legged tick (Ixodes scapularis) were also identified. In both years of our study, 60.9% of ticks tested positive for 17-kDa. "Candidatus Rickettsia amblyommii", identified in all four tick species, accounted for 90.2% (416/461) of the 23S-5S-positive samples and 52.9% (416/787) of all samples tested. Nucleotide sequence analysis of Rickettsia-specific 23S-5S IGS, ompA and gltA gene fragments indicated that ticks, principally A. americanum, contained novel species of Rickettsia. Other Rickettsiales, including Ehrlichia ewingii, E. chaffeensis, Ehrlichia sp. (Panola Mountain), and Anaplasma phagocytophilum, were infrequently identified, principally in A. americanum.We conclude that in North Carolina, the most common rickettsial exposure is to R. amblyommii carried by A. americanum. Other Rickettsiales bacteria, including novel species of Rickettsia, were less frequently detected in A. americanum but are relevant to public health nevertheless.}, journal={PARASITES & VECTORS}, author={Lee, Sangmi and Kakumanu, Madhavi L. and Ponnusamy, Loganathan and Vaughn, Meagan and Funkhouser, Sheana and Thornton, Haley and Meshnick, Steven R. and Apperson, Charles S.}, year={2014}, month={Dec} }