@article{brannan_livingston_wall_livingston_rensburg_2021, title={Embryonic thermal manipulation and dietary fat source during acute heat stress: 2. Effect on broiler carcass characteristics and breast muscle myopathies}, volume={30}, ISSN={["1537-0437"]}, DOI={10.1016/j.japr.2021.100174}, abstractNote={The objective of this study was to evaluate the effect of embryonic thermal manipulation and dietary fat source during the finisher period on carcass characteristics and breast muscle myopathies following acute heat stress. Thermal manipulation (TM) of incubation temperatures has been shown to improve bird resistance to heat stress and enhance breast muscle growth. Fat supplementation is frequently used during heat stress and fat source may alter carcass composition, as fat sources rich in unsaturated fatty acids have been shown to reduce fat pad weight. Ross 708 eggs were incubated at 37.5°C except during TM when temperature was increased to 39.5°C for 12 h daily from embryo day 7 to 16. A total of 1,080 chicks were reared under standard conditions until acute heat stress at 43 d. Dietary treatments were applied during the finisher period beginning at 28 d, with diets including 4.5% of soya oil, poultry fat, or olive oil. At 49 d, 240 male birds were processed to assess carcass and portion weights, as well as breast quality. Carcass and portion weights were decreased by TM, however percent yield was similar to the controls. A decrease in breast muscle myopathies was noted but may have been due to the lower BW of the TM birds. Interactions between the treatments suggest that TM may alter lipid metabolism. Differences in dietary fat source did not affect carcass characteristics. The reduction in breast muscle myopathies may be negated by the negative impact of TM on carcass weights.}, number={3}, journal={JOURNAL OF APPLIED POULTRY RESEARCH}, author={Brannan, K. E. and Livingston, M. L. and Wall, B. and Livingston, K. A. and Rensburg, C. Jansen}, year={2021}, month={Sep} }
 @article{joardar_livingston_edens_nusairat_qudsieh_livingston_brake_2020, title={Effect of Limestone Particle Size and Potassium Supplementation on Growth Performance, Blood Physiology, and Breast Muscle Myopathy of Male Broiler Chickens}, volume={7}, ISSN={["2297-1769"]}, DOI={10.3389/fvets.2020.603284}, abstractNote={The experiment investigated the effects of limestone particle size and dietary potassium (K) on live performance, blood physiology, and muscle myopathies in broilers raised to 35 days of age. A total of 384 Ross male broilers were placed in 24 floor pens and fed four diets during the starter (0–16 days of age) and grower (17–33 days of age) periods containing two limestone particle sizes (fine: 0.2 mm and coarse: 0.9 mm), and amended with either 0% basal K (K–) or 0.2% added dietary K (K+) as potassium carbonate to complete the 2 × 2 factorial arrangement. Live performance was measured from 1–33 days of age. Blood physiology, woody breast (WB), and white striping (WS) scores were measured at 35 days of age. The K+ dietary treatment reduced (P < 0.05) feed intake and BWG when compared to K– during the starter and grower period. The K+ dietary treatment decreased blood Na (mmol/L), blood glucose (mg/dl), ionized blood Ca (mg/dl), TCO2 (mmol/L), blood HCO3 (mmol/L), and base excess in extracellular fluid (mmol/L) when compared to K– birds of similar body weight at 35 days of age (P ≤ 0.05). Fine limestone diets tended to reduce WB scores (3.0 vs. 2.59) when compared to coarse limestone diets at 35 days of age (P = 0.08). This study demonstrated that using 0.2% of K as potassium carbonate did not negatively affect FCR even though FI and BWG were reduced. Furthermore, fine limestone has the potential to reduce WB in breast muscle tissues; however, further research is needed to confirm these outcomes.}, journal={FRONTIERS IN VETERINARY SCIENCE}, author={Joardar, Dinabandhu and Livingston, Kimberly A. and Edens, Frank W. and Nusairat, Basheer and Qudsieh, Rasha and Livingston, Matthew L. and Brake, John}, year={2020}, month={Dec} }
 @article{livingston_cowieson_crespo_hoang_nogal_browning_livingston_2020, title={Effect of broiler genetics, age, and gender on performance and blood chemistry}, volume={6}, ISSN={["2405-8440"]}, url={http://www.scopus.com/inward/record.url?eid=2-s2.0-85087691199&partnerID=MN8TOARS}, DOI={10.1016/j.heliyon.2020.e04400}, abstractNote={A total of 640 broilers were used to determine the effects of strain, sex, and age on hematology and blood chemistry using rapid detection devices. Day old chicks from two genetic lines of common fast-growing and high-yield broiler strains were sexed and allocated to 40 pens (16 birds per pen) containing either male or female and Ross or Cobb strains (n = 10). Venous blood was analyzed weekly using 2 broilers from each pen (n = 20) using the i-STAT® Alinity Handheld Clinical Analyzer, Zoetis Vetscan VS2, and iCheck™ Carotene devices at 14, 21, 28, 35, and 42 d, as well as growth performance. Post-mortem health tracking metrics were also recorded on 42 d. Broilers were deemed healthy based on posting data results and performed in accordance with industry standards with males presenting greater BW and reduced FCR than female broilers. Ross broilers displayed greater BW to 14 d with similar FCR compared with Cobb birds. Day of age had a highly significant impact on blood calcium, phosphorus, potassium, sodium, chloride, carotene, aspartate aminotransferase, creatine kinase, bile acids, uric acid, total protein, albumin, globulin, total carbon dioxide, hematocrit, and malondialdehyde. Male broilers had reduced blood sodium, chloride, carotene, uric acid, albumin, and increased total protein, glucose, and total carbon dioxide. Ross broilers had greater blood potassium, and sodium, as well as reduced uric acid, total protein, globulin, and malondialdehyde, compared with Cobb birds. These results demonstrated the effectiveness of point-of-care devices in measuring blood chemistry and hematology in modern broilers. These data can be utilized to determine normal healthy blood ranges in these types of broilers when accounting for strain, sex, and age.}, number={7}, journal={HELIYON}, author={Livingston, M. L. and Cowieson, A. J. and Crespo, R. and Hoang, V and Nogal, B. and Browning, M. and Livingston, K. A.}, year={2020}, month={Jul} }
 @article{cowieson_livingston_nogal_hoang_wang_crespo_livingston_2020, title={Effect of coccidial challenge and vaccination on the performance, veterinary postmortem scores, and blood biochemistry of broiler chickens}, volume={99}, ISSN={["1525-3171"]}, url={http://www.scopus.com/inward/record.url?eid=2-s2.0-85087699065&partnerID=MN8TOARS}, DOI={10.1016/j.psj.2020.05.018}, abstractNote={A total of 960 male Cobb 500 broilers were used in a growth performance study to explore the effect of coccidial vaccination and/or coccidial challenge on blood biochemistry and veterinary postmortem metrics. Day-old chicks were randomly divided into one of the 4 experimental treatments. Treatments were arranged in a 2 × 2 factorial arrangement, with the factors being without or with vaccination (administered on day 1) or coccidial challenge (oral gavage on day 7). Growth performance was monitored on a weekly basis. Blood sample collection, as well as full veterinary necropsies, were carried out on days 6, 8, 13, 20, 27, and 34. Birds that did not receive the vaccination but were challenged with coccidiosis had higher feed conversion ratio, lower body weights, and higher mortality than the other experimental groups, and this effect was particularly evident from day 13 to day 20. Birds challenged with coccidiosis had lower plasma sodium and total carotenoid concentrations and higher potassium and globulin concentrations than nonchallenged birds. Significant interactions between age and experimental treatment for these blood parameters were observed, particularly on day 13. The necropsy results confirmed the effectiveness of the challenge and vaccination treatments, wherein birds that were challenged had higher coccidiosis scores on day 13 and day 27 than birds that were not challenged. These results demonstrate the potential for plasma sodium, potassium, total protein, total carbon dioxide, globulin, and carotenoid analysis for early diagnosis of coccidiosis in growing broiler chickens. Further work is necessary to establish whether the changes in blood biochemistry observed in the present study are transferable to alternative flocks of chicken and whether early diagnosis and intervention may mitigate performance losses associated with this disease.}, number={8}, journal={POULTRY SCIENCE}, author={Cowieson, A. J. and Livingston, M. L. and Nogal, B. and Hoang, V and Wang, Y-T and Crespo, R. and Livingston, K. A.}, year={2020}, month={Aug}, pages={3831–3840} }
 @article{phillips_reading_livingston_livingston_ashwell_2020, title={Evaluation via Supervised Machine Learning of the Broiler Pectoralis Major and Liver Transcriptome in Association With the Muscle Myopathy Wooden Breast}, volume={11}, ISSN={["1664-042X"]}, DOI={10.3389/fphys.2020.00101}, abstractNote={The muscle myopathy wooden breast (WB) has recently appeared in broiler production and has a negative impact on meat quality. WB is described as hard/firm consistency found within the pectoralis major (PM). In the present study, we use machine learning from our PM and liver transcriptome dataset to capture the complex relationships that are not typically revealed by traditional statistical methods. Gene expression data was evaluated between the PM and liver of birds with WB and those that were normal. Two separate machine learning algorithms were performed to analyze the data set including the sequential minimal optimization (SMO) of support vector machines (SVMs) and Multilayer Perceptron (MLP) Artificial Neural Network (ANN). Machine learning algorithms were compared to identify genes within a gene expression data set of approximately 16,000 genes for both liver and PM, which can be correctly classified from birds with or without WB. The performance of both machine learning algorithms SMO and MLP was determined using percent correct classification during the cross-validations. By evaluating the WB transcriptome datasets by 5× cross-validation using ANNs, the expression of nine genes ranked based on Shannon Entropy (Information Gain) from PM were able to correctly classify if the individual bird was normal or exhibited WB 100% of the time. These top nine genes were all protein coding and potential biomarkers. When PM gene expression data were evaluated between normal birds and those with WB using SVMs they were correctly classified 95% of the time using 450 of the top genes sorted ranked based on Shannon Entropy (Information Gain) as a preprocessing step. When evaluating the 450 attributes that were 95% correctly classified using SVMs through Ingenuity Pathway Analysis (IPA) there was an overlap in top genes identified through MLP. This analysis allowed the identification of critical transcriptional responses for the first time in both liver and muscle during the onset of WB. The information provided has revealed many molecules and pathways making up a complex molecular mechanism involved with the progression of wooden breast and suggests that the etiology of the myopathy is not limited to activity in the muscle alone, but is an altered systemic pathology.}, journal={FRONTIERS IN PHYSIOLOGY}, author={Phillips, Chelsea A. and Reading, Benjamin J. and Livingston, Matthew and Livingston, Kimberly and Ashwell, Chris M.}, year={2020}, month={Feb} }
 @article{toomer_sanders_vu_livingston_wall_malheiros_carvalho_livingston_ferket_anderson_2020, title={Potential Transfer of Peanut and/or Soy Proteins from Poultry Feed to the Meat and/or Eggs Produced}, volume={5}, ISSN={2470-1343 2470-1343}, url={http://dx.doi.org/10.1021/acsomega.9b03218}, DOI={10.1021/acsomega.9b03218}, abstractNote={Previous studies have demonstrated that allergenic feed proteins from peanuts in the diets of layer hens are not detected in the eggs produced. Hence, in this study, we aimed to determine if soy and/or peanut proteins in poultry feed rations of broiler chickens or layer hens would be transferred or detectable in the meat or eggs produced. To meet this objective, 99 layer hens and 300 broiler chickens were equally divided into treatment groups and fed one of three experimental diets: control soybean meal and corn diet, whole unblanched high-oleic peanut and corn diet (HO PN), or a control diet spiked supplemented with oleic acid (OA) oil. At termination, broiler chickens were processed, and chicken breast samples of the left pectoralis muscle were collected, and eggs were collected from layers. Total protein extracts from pooled egg samples and chicken breast samples were subjected to enzyme-linked immunosorbent assay (ELISA) methods and immunoblotting analysis with rabbit antipeanut agglutinin antibodies and rabbit antisoy antibodies for the detection of peanut and soy proteins. Peanut and soy proteins were undetected in all pooled egg samples and individual chicken breast meat samples using immunoblotting techniques with rabbit antipeanut agglutinin and rabbit antisoy antibodies. Moreover, quantitative ELISA allergen detection methods determined all pooled egg samples and individual meat samples as “not containing” peanut or soy allergens. Therefore, this study helps to evaluate the risk associated with the potential transfer of allergenic proteins from animal feed to the products produced for human consumption.}, number={2}, journal={ACS Omega}, publisher={American Chemical Society (ACS)}, author={Toomer, Ondulla T. and Sanders, Elliot and Vu, Thien C. and Livingston, Matthew L. and Wall, Brittany and Malheiros, Ramon D. and Carvalho, Luiz Victor and Livingston, Kim A. and Ferket, Peter R. and Anderson, Kenneth E.}, year={2020}, month={Jan}, pages={1080–1085} }
 @article{toomer_sanders_vu_malheiros_redhead_livingston_livingston_carvalho_ferket_2020, title={The effects of high-oleic peanuts as an alternative feed ingredient on broiler performance, ileal digestibility, apparent metabolizable energy, and histology of the intestine}, volume={4}, ISSN={["2573-2102"]}, DOI={10.1093/tas/txaa137}, abstractNote={Locally grown feed ingredients of high energy and protein content, such as peanuts, maybe economically feasible alternatives to corn and soybean meal in broiler diets. Even though normal-oleic peanuts have been demonstrated to be a viable feed ingredient for poultry, few studies to date have examined the use of high-oleic peanuts (HO PN) as an alternative feed ingredient for broiler chickens. Thus, we aimed to determine the effect of feeding HO PN on broiler performance, nutrient digestibility, and intestinal morphology. Three isocaloric, isonitrogenous experimental diets were formulated with 1) dietary inclusion of ~10% coarse-ground whole HO PN; 2) a corn-soybean meal control diet with 5.5% added poultry fat; and 3) a control diet supplemented with 5.5% oleic fatty acid oil. Three-hundred Ross 708 broilers were randomly placed in 10 replicate pens per treatment with 10 chicks per pen and raised until 42 d. Body weights (BW) and feed intake were determined weekly, and feed conversion ratio (FCR) was calculated. Jejunum samples were collected at 42 d for histomorphometric analysis. Analysis of variance was performed on all variables using a general linear mixed model in JMP Pro14. Broilers in the HO PN group had lower (P < 0.05) BW and higher FCR than other treatment groups at weeks 2 and 6. There were no significant differences in the jejunum villi surface area between the treatment groups. However, broilers fed the HO PN diet had greater (P = 0.019) apparent metabolizable energy relative to the other treatment groups, suggesting improved nutrient uptake of dietary fats and/or carbohydrates in the HO PN treatment group. However, additional studies are warranted to further define the nutritional value of HO PN as an alternative poultry feed ingredient.}, number={3}, journal={TRANSLATIONAL ANIMAL SCIENCE}, author={Toomer, Ondulla T. and Sanders, Elliot and Vu, Thien C. and Malheiros, Ramon D. and Redhead, Adam K. and Livingston, Matthew L. and Livingston, Kim A. and Carvalho, Luiz Victor and Ferket, Peter R.}, year={2020}, month={Jul} }
 @article{toomer_livingston_wall_sanders_vu_malheiros_livingston_carvalho_ferket_2019, title={Meat quality and sensory attributes of meat produced from broiler chickens fed a high oleic peanut diet}, volume={98}, ISSN={0032-5791}, url={http://dx.doi.org/10.3382/ps/pez258}, DOI={10.3382/ps/pez258}, abstractNote={Previous studies have identified peanut meal prepared from normal-oleic peanuts as a suitable and economical ingredient for broiler feed. However, to date, no studies have examined the use of new, high-oleic peanut (HO-PN) cultivars as a feed ingredient for poultry. This project aimed to determine the effect of HO-PNs as a feed ingredient for broiler chickens on the quality and sensory attributes of the meat produced. To test 3 experimental diets, male broiler chicks were randomly placed, at hatch, in raised-wire cages, in 10 replicate pens per treatment with 10 chicks per cage. For 6 wk, chicks were fed, ad libitum, one of the three isocaloric, isonitrogenous diets: (1) a conventional soybean meal plus corn control diet, (2) 10 to 12% HO-PN + corn diet, or (3) a control corn diet spiked with ≈6.0% oleic fatty acid oil (OA). At 42 D, 3 broilers per pen (30 per treatment) were processed to determine meat quality and for consumer evaluation. Carcass weights and breast yields were reduced in broilers fed HO-PN, while leg carcass yields were greater in broilers fed HO-PN in comparison to the other groups. Chicken breast from broilers fed HO-PN had reduced meat-pH, reduced L* color values, and increased cooked loss compared to other treatments. Nevertheless, a group of 100-consumer panelists scored all 3-treatment groups similar in terms of sensory attributes for cooked chicken. While additional studies must be performed, this study suggest that HO-PN may be a suitable broiler feed ingredient.}, number={10}, journal={Poultry Science}, publisher={Elsevier BV}, author={Toomer, Ondulla T and Livingston, Matthew L and Wall, Brittany and Sanders, Elliot and Vu, Thien C and Malheiros, Ramon D and Livingston, Kim A and Carvalho, Luiz Victor and Ferket, Peter R}, year={2019}, month={Oct}, pages={5188–5197} }
 @article{livingston_ferket_brake_livingston_2018, title={Dietary amino acids under hypoxic conditions exacerbates muscle myopathies including wooden breast and white stripping}, volume={98}, ISSN={0032-5791 1525-3171}, url={http://dx.doi.org/10.3382/ps/pey463}, DOI={10.3382/ps/pey463}, abstractNote={ABSTRACT The occurrence of wooden breast (WB) and white striping (WS) of broiler breast myopathies may be associated with least‐cost feed formulations and the inclusion of greater amounts of synthetic amino acids. Two experiments were conducted to evaluate the effects of supplemental glutamine (Gln‐/+, 0 and 1%) and arginine (Arg‐/+, 0 and 0.25%) in a 2 × 2 factorial arrangement. Experiment 1 consisted of 8 replicates using 32 pens and focused on live performance to 44 d of age, carcass yield, and meat quality evaluations at 45 d of age. Live performance parameters including feed intake, BW gain, mortality, and feed conversion were evaluated. Post‐harvest carcass yield, breast muscle quality (including WB and WS), proximate analysis, and mineral analysis of breast muscle and tibia bone were also evaluated. Experiment 2 comprised 4 replicates using 8 pens with weekly measurements of blood physiology and muscle myopathy development from 21 to 45 d of age. Broilers fed supplemental Gln and Arg (Gln+/Arg+) produced broilers with greater BW and improved FCR. The WB and WS severity increased with Gln+ diets, while only WB increased using Arg+ diets. Weekly observations revealed the greatest increase in WB myopathies occurred between 28 to 35 d, while WS significantly increased one week later (35 to 42 d). When comparing broilers of similar BW at 45 d, Gln+ diets resulted in greater WS scores and percentage breast muscle fat. Further analysis of WB and WS scores (independent of treatment affect) revealed that increased myopathy scores were associated with increased blood CO2, H2CO3, and reduced O2 with increased meat pH, moisture content, and Ca concentration. These results indicated that increased muscle Ca is associated with increased WB, while increased dietary glutamine and lack of oxygen may have resulted in a reverse flux of the citric acid cycle and reduced electron transport chain activity resulting in increased WS.}, number={3}, journal={Poultry Science}, publisher={Elsevier BV}, author={Livingston, M L and Ferket, P R and Brake, J and Livingston, K A}, year={2018}, month={Oct}, pages={1517–1527} }