Michelle Lynn Luo

Works (7)

Updated: July 5th, 2023 15:42

2016 journal article

Engineering genes with CRISPR-Cas9

Chemical Engineering Progress , 112(9), 36–41.

By: M. Luo & C. Beisel

Source: NC State University Libraries
Added: August 6, 2018

2016 journal article

The CRISPR RNA-guided surveillance complex in Escherichia coli accommodates extended RNA spacers

NUCLEIC ACIDS RESEARCH, 44(15), 7385–7394.

By: M. Luo n, R. Jackson *, S. Denny n, M. Tokmina-Lukaszewska *, K. Maksimchuk n, W. Lin*, B. Bothner *, B. Wiedenheft *, C. Beisel n

MeSH headings : CRISPR-Associated Proteins / metabolism; CRISPR-Cas Systems / genetics; DNA / metabolism; Escherichia coli / genetics; Escherichia coli Proteins / metabolism; Gene Expression Regulation, Bacterial; Gene Silencing; Macromolecular Substances / chemistry; Macromolecular Substances / metabolism; RNA, Bacterial / chemistry; RNA, Bacterial / genetics; RNA, Guide, Kinetoplastida / chemistry; RNA, Guide, Kinetoplastida / genetics; Transcription, Genetic
TL;DR: It is found that extending the spacer portion of the crRNA resulted in larger Cascade complexes with altered stoichiometry and preserved in vitro binding affinity for target DNA, and spacer length can be modified to fine-tune Cascade activity. (via Semantic Scholar)
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Source: Web Of Science
Added: August 6, 2018

2015 review

Current and future prospects for CRISPR-based tools in bacteria

[Review of ]. BIOTECHNOLOGY AND BIOENGINEERING, 113(5), 930–943.

By: M. Luo n, R. Leenay n & C. Beisel n

author keywords: antimicrobials; Cas9; genetic control; genetic circuits; genome engineering; undomesticated microbes
MeSH headings : Anti-Bacterial Agents / pharmacology; Bacteria / drug effects; Bacteria / genetics; Bacterial Infections / drug therapy; CRISPR-Cas Systems; Clustered Regularly Interspaced Short Palindromic Repeats; Drug Discovery; Drug Resistance, Bacterial; Gene Editing / methods; Gene Expression Regulation, Bacterial; Genetic Engineering / methods; Genome, Bacterial; Humans
TL;DR: Overall, CRISPR‐Cas systems have formed the basis of an ever‐expanding genetic toolbox and hold tremendous potential for the future understanding and engineering of the bacterial world. (via Semantic Scholar)
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Source: Web Of Science
Added: August 6, 2018

2015 journal article

Impact of Residual Inducer on Titratable Expression Systems

PLOS ONE, 10(9).

By: T. Afroz n, M. Luo n & C. Beisel n

MeSH headings : Arabinose / biosynthesis; Arabinose / genetics; Bacterial Proteins / biosynthesis; Bacterial Proteins / genetics; Escherichia coli; Flow Cytometry; Gene Expression Regulation, Bacterial; Models, Theoretical; Plasmids; Promoter Regions, Genetic; Riboswitch / genetics; Thiamine / genetics; Thiamine Pyrophosphate / biosynthesis; Thiamine Pyrophosphate / genetics
TL;DR: It is found that residual inducer had a distinct effect on “all-or-none” systems, which exhibited increased sensitivity to the added inducer until becoming fully induced. (via Semantic Scholar)
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Source: Web Of Science
Added: August 6, 2018

2014 journal article

Programmable Removal of Bacterial Strains by Use of Genome-Targeting CRISPR-Cas Systems

MBIO, 5(1).

By: A. Gomaa n, H. Klumpe  n, M. Luo n, K. Selle n, R. Barrangou n  & C. Beisel n

MeSH headings : CRISPR-Cas Systems; DNA, Bacterial / genetics; DNA, Bacterial / metabolism; Escherichia coli / genetics; Genome, Bacterial; Hydrolysis; Microbial Viability
TL;DR: It is demonstrated that genome targeting with CRISPR-Cas systems can be employed for the sequence-specific and titratable removal of individual bacterial strains and species and may open new avenues for the development of “smart” antibiotics that circumvent multidrug resistance and differentiate between pathogenic and beneficial microorganisms. (via Semantic Scholar)
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Sources: Web Of Science, ORCID
Added: August 6, 2018

2014 journal article

Repurposing endogenous type I CRISPR-Cas systems for programmable gene repression

NUCLEIC ACIDS RESEARCH, 43(1), 674–681.

By: M. Luo n, A. Mullis n, R. Leenay n & C. Beisel n

MeSH headings : CRISPR-Associated Proteins / genetics; CRISPR-Cas Systems; Cell Engineering; Escherichia coli / genetics; Gene Deletion; Transcription, Genetic
TL;DR: It is found that deletion of the signature cas3 gene converted this immune system in Escherichia coli into a programmable gene regulator capable of reversible gene silencing of heterologous and endogenous genes. (via Semantic Scholar)
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Source: Web Of Science
Added: August 6, 2018

2013 journal article

Embedded collagen deformation models for computational modeling of healthy, keratoconic, and crosslinked corneas

Journal of Mechanics in Medicine and Biology, 13(3).

By: C. Foster, D. Gongal, T. Begaj & M. Luo

Source: NC State University Libraries
Added: August 6, 2018

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