Mans Ekelof

Works (13)

Updated: July 16th, 2023 21:13

2020 journal article

3D Imaging and metabolomic profiling reveal higher neuroactive kavalactone contents in lateral roots and crown root peels of Piper methysticum (kava)

GIGASCIENCE, 9(9).

By: Y. Jaiswal *, A. Yerke *, M. Bagley  n, M. Ekelof n, D. Weber *, D. Haddad *, A. Fodor *, D. Muddiman n , L. Williams*

author keywords: kava; kavalactones; metabolomics; 3D imaging; mass spectrometry imaging
MeSH headings : Imaging, Three-Dimensional; Kava; Lactones; Metabolomics; X-Ray Microtomography
TL;DR: Detailed metabolomic profile and spatio-temporal characteristics of tissues from the roots and stems are characterized using cross-platform metabolomics and a 3D imaging approach to provide mechanistic insights into the social and clinical practice of the use of only peeled roots. (via Semantic Scholar)
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Sources: Web Of Science, NC State University Libraries
Added: November 16, 2020

2020 journal article

A Versatile Platform for Mass Spectrometry Imaging of Arbitrary Spatial Patterns

Journal of the American Society for Mass Spectrometry, 31(12), 2547–2552.

By: K. Garrard n, M. Ekelöf n, S. Khodjaniyazova n, M. Bagley  n & D. Muddiman n 

author keywords: Mass Spectrometry Imaging; Arbitrary Region of Interest; MALDESI; Vision-System
TL;DR: A vision-system driven platform, RastirX, has been constructed for mass spectrometry imaging (MSI) of arbitrary two-dimensional patterns that reduces contamination from off-sample material and significantly reduces acquisition time. (via Semantic Scholar)
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Sources: Web Of Science, NC State University Libraries, Crossref
Added: January 19, 2021

2020 journal article

Determination of Optimal Electrospray Parameters for Lipidomics in Infrared-Matrix-Assisted Laser Desorption Electrospray Ionization Mass Spectrometry Imaging

JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY, 31(2), 319–325.

By: M. Bagley  n, M. Ekelof n & D. Muddiman n 

author keywords: IR-MALDESI; mass spectrometry imaging: electrospray ionization; metabolites; lipids
MeSH headings : Animals; Lipidomics / methods; Lipids / analysis; Liver / chemistry; Liver / diagnostic imaging; Molecular Imaging / methods; Rats; Spectrometry, Mass, Electrospray Ionization / methods; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods
TL;DR: Using these defined parameters, IR-MALDESI positive polarity lipidomics studies can increase lipid abundances 3-fold, with 15% greater coverage, while an abundance increase of 1.5-fold and 10% more coverage can be achieved relative to commonly used parameters in negative polarity. (via Semantic Scholar)
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6. Clean Water and Sanitation (OpenAlex)
Sources: Web Of Science, NC State University Libraries
Added: March 10, 2020

2020 journal article

Infrared matrix-assisted laser desorption electrospray ionization (IR-MALDESI) mass spectrometry imaging analysis of endogenous metabolites in cherry tomatoes

ANALYST, 145(16), 5516–5523.

By: M. Bagley, C. Pace*, M. Ekelof* & D. Muddiman n 

MeSH headings : Lasers; Lipids; Solanum lycopersicum; Spectrometry, Mass, Electrospray Ionization; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
TL;DR: The spatially resolved metabolic profiling of cherry tomatoes using infrared matrix-assisted laser desorption electrospray ionization (IR-MALDESI), a mass spectrometry imaging technique that operates at ambient conditions and requires no sample derivatization, finds endogenous carotenoid hydrocarbons ionized as radical cations. (via Semantic Scholar)
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Sources: Web Of Science, NC State University Libraries
Added: August 31, 2020

2019 journal article

Development of a relative quantification method for infrared matrix‐assisted laser desorption electrospray ionization mass spectrometry imaging of <i>Arabidopsis</i> seedlings

Rapid Communications in Mass Spectrometry, 34(6).

By: M. Bagley  n, A. Stepanova n , M. Ekelöf n, J. Alonso  n & D. Muddiman n 

MeSH headings : Arabidopsis / chemistry; Arabidopsis / genetics; Arabidopsis Proteins / genetics; Indoleacetic Acids / analysis; Indoles / analysis; Mutation; Oximes / analysis; Oxygenases / genetics; Seedlings / chemistry; Seedlings / genetics; Spectrometry, Mass, Electrospray Ionization / methods; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods
TL;DR: IAA doping of agarose was demonstrated as a viable technique for relative quantification of metabolites in live seedlings or tissues with similar biological considerations and was shown to be an appropriate sampling surface for IR-MALDESI mass spectrometry imaging of Arabidopsis seedlings. (via Semantic Scholar)
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Sources: Web Of Science, NC State University Libraries, Crossref
Added: March 10, 2020

2018 journal article

Characterization of a novel miniaturized burst-mode infrared laser system for IR-MALDESI mass spectrometry imaging

ANALYTICAL AND BIOANALYTICAL CHEMISTRY, 410(9), 2395–2402.

By: M. Ekelof n, J. Manni, M. Nazari  n, M. Bokhart  n & D. Muddiman n 

author keywords: Mass spectrometry imaging; Infrared laser; IR-MALDESI; HRAM mass spectrometry
MeSH headings : Animals; Equipment Design; Infrared Rays; Lasers; Mice; Miniaturization / instrumentation; Optical Imaging / instrumentation; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / instrumentation; Whole Body Imaging / instrumentation
TL;DR: The use of a novel miniature 2.94 μm burst-mode laser in IR-MALDESI allows for rapid and sensitive mass spectrometry imaging of a whole mouse, yielding several hundred confident metabolite identifications. (via Semantic Scholar)
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Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2018 journal article

Discovery and quantification of bioactive peptides in fermented cucumber by direct analysis IR-MALDESI mass spectrometry and LC-QQQ-MS

FOOD CHEMISTRY, 271, 715–723.

By: J. Fideler n, S. Johanningsmeier n, M. Ekelof n & D. Muddiman n 

author keywords: Lactic acid fermentation; ACE-inhibitory peptides; Isoleucine-proline-proline; Leucine-proline-proline; Valine-proline-proline; Vegetable composition
MeSH headings : Cucumis sativus / chemistry; Fermentation; Peptides / chemistry; Sodium Chloride; Tandem Mass Spectrometry
TL;DR: It is demonstrated that lactic acid bacteria fermentation can enhance bioactive peptide content in vegetables by 3-5 fold in fermented cucumbers compared with acidified cucumbers. (via Semantic Scholar)
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Sources: Web Of Science, NC State University Libraries
Added: October 16, 2018

2018 journal article

Evaluation of Digital Image Recognition Methods for Mass Spectrometry Imaging Data Analysis

Journal of The American Society for Mass Spectrometry, 29(12), 2467–2470.

By: M. Ekelöf n, K. Garrard n, R. Judd n, E. Rosen*, D. Xie n , A. Kashuba *, D. Muddiman n 

author keywords: Mass spectrometry imaging; Image recognition; SSIM
MeSH headings : Algorithms; Animals; Image Processing, Computer-Assisted / methods; Lymph Nodes / chemistry; Lymph Nodes / metabolism; Macaca mulatta; Maraviroc / pharmacokinetics; Mass Spectrometry / methods; Models, Theoretical; Molecular Imaging / methods; Plant Leaves / chemistry; Tissue Distribution
TL;DR: A method for comprehensive, semi-targeted discovery of molecular distributions of interest from mass spectrometry imaging data, using widely available image similarity scoring algorithms to rank images by spatial correlation is presented. (via Semantic Scholar)
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Sources: Web Of Science, Crossref, NC State University Libraries
Added: December 10, 2018

2018 journal article

IR-MALDESI mass spectrometry imaging of underivatized neurotransmitters in brain tissue of rats exposed to tetrabromobisphenol A

ANALYTICAL AND BIOANALYTICAL CHEMISTRY, 410(30), 7979–7986.

By: M. Bagley  n, M. Ekelof n, K. Rock n, H. Patisaul n  & D. Muddiman n 

author keywords: IR-MALDESI; Neurotransmitters; Mass spectrometry imaging; Exposomics; Orbitrap
MeSH headings : Animals; Brain; Female; Infrared Rays; Neurotransmitter Agents / chemistry; Polybrominated Biphenyls / toxicity; Rats; Spectrometry, Mass, Electrospray Ionization / methods; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods
TL;DR: This study serves as a demonstration of a new application of IR-MALDESI, namely that neurotransmitter distributions can be confidently and rapidly imaged without derivatization, and is used for the analysis of neurotransmitters in the brains of rats exposed to TBBPA in gestation and lactation through their mothers. (via Semantic Scholar)
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3. Good Health and Well-being (Web of Science; OpenAlex)
Sources: Web Of Science, NC State University Libraries
Added: December 3, 2018

2017 journal article

Direct analysis of terpenes from biological buffer systems using SESI and IR-MALDESI

Analytical and Bioanalytical Chemistry, 410(3), 953–962.

By: M. Nazari  n, A. Malico n, M. Ekelöf n, S. Lund n, G. Williams n  & D. Muddiman n 

author keywords: Terpenes; IR-MALDESI; SESI; Direct analysis; Biological buffers; Q Exactive Plus
MeSH headings : Alkenes / analysis; Buffers; Infrared Rays; Ions / analysis; Monocyclic Sesquiterpenes; Sesquiterpenes / analysis; Spectrometry, Mass, Electrospray Ionization / methods; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods; Terpenes / analysis
TL;DR: By coupling the two techniques and doping the electrospray solvent with silver ions, olefinic terpenes of different classes and varying degrees of volatility were directly analyzed from a biological buffer system with no sample workup steps. (via Semantic Scholar)
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Sources: Web Of Science, NC State University Libraries, Crossref
Added: August 6, 2018

2017 journal article

Direct screening of enzyme activity using infrared matrix-assisted laser desorption electrospray ionization

RAPID COMMUNICATIONS IN MASS SPECTROMETRY, 31(22), 1868–1874.

By: M. Nazari n, M. Ekelof n, S. Khodjaniyazova n, N. Elsen *, J. Williams * & D. Muddiman n 

MeSH headings : Drug Discovery / methods; Enzyme Assays / methods; High-Throughput Screening Assays / methods; Isocitrate Dehydrogenase / metabolism; Isocitrates / metabolism; Ketoglutaric Acids / metabolism; Models, Chemical; NADP / metabolism; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods
TL;DR: The utility of infrared matrix-assisted laser desorption electrospray ionization for monitoring an enzymatic reaction directly from a biological buffer system with no sample cleanup and at high throughput is presented. (via Semantic Scholar)
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Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2017 journal article

IR-MALDESI Mass Spectrometry Imaging at 50 Micron Spatial Resolution

Journal of The American Society for Mass Spectrometry, 28(10), 2099–2107.

By: M. Bokhart n, J. Manni, K. Garrard n, M. Ekelöf n, M. Nazari n & D. Muddiman n 

author keywords: Mass spectrometry imaging; IR-MALDESI; Tissue imaging; Spatial resolution; Laser focusing
TL;DR: This work presents MSI using infrared matrix-assisted laser desorption electrospray ionization (IR-MALDESI) at 50 μm resolution, using an adjustable iris, beam expander, and an aspherical focusing lens to reduce tissue ablation diameters for MSI at high resolution. (via Semantic Scholar)
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Sources: Web Of Science, Crossref, NC State University Libraries
Added: August 6, 2018

2017 journal article

IR-MALDESI method optimization based on time-resolved measurement of ion yields

ANALYTICAL AND BIOANALYTICAL CHEMISTRY, 410(3), 963–970.

By: M. Ekeloef & D. Muddiman n 

author keywords: IR-MALDESI; Electrospray post-ionization; Mass spectrometry imaging; Laser ablation; Q Exactive
MeSH headings : Animals; Brain Chemistry; Cholesterol / analysis; Ions / analysis; Laser Therapy / methods; Lipids / analysis; Liver / chemistry; Mice; Rats; Spectrometry, Mass, Electrospray Ionization / methods; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods
TL;DR: In a model imaging experiment using infrared matrix-assisted laser desorption electrospray ionization, fixing the injection time at the minimum duration required to capture all ions generated by the last laser pulse in a sequence is shown to maximize target ion abundances. (via Semantic Scholar)
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Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

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