@article{xi_patel_dong_que_qu_2018, title={Acetosyringone treatment duration affects large T-DNA molecule transfer to rice callus}, volume={18}, ISSN={["1472-6750"]}, DOI={10.1186/s12896-018-0459-5}, abstractNote={Large T-DNA fragment transfer has long been a problem for Agrobacterium-mediated transformation. Although vector systems, such as the BIBAC series, were successfully developed for the purpose, low transformation efficiencies were consistently observed. To gain insights of this problem in monocot transformation, we investigated the T-strand accumulation of various size of T-DNA in two kinds of binary vectors (one copy vs. multi-copy) upon acetosyringone (AS) induction and explored ways to improve the efficiency of the large T-DNA fragment transfer in Agrobacterium-mediated rice transformation. By performing immuno-precipitation of VirD2-T-strands and quantitative real-time PCR assays, we monitored the accumulation of the T-strands in Agrobacterium tumeficiens after AS induction. We further demonstrated that extension of AS induction time highly significantly improved large-size T-DNA transfer to rice cells. Our data provide valuable information of the T-strand dynamics and its impact on large T-DNA transfer in monocots, and likely dicots as well.}, journal={BMC BIOTECHNOLOGY}, author={Xi, Jing and Patel, Minesh and Dong, Shujie and Que, Qiudeng and Qu, Rongda}, year={2018}, month={Aug} }
 @article{patel_milla-lewis_zhang_templeton_reynolds_richardson_biswas_zuleta_dewey_qu_et al._2015, title={Overexpression of ubiquitin-like LpHUB1 gene confers drought tolerance in perennial ryegrass}, volume={13}, ISSN={["1467-7652"]}, DOI={10.1111/pbi.12291}, abstractNote={SummaryHUB1, also known as Ubl5, is a member of the subfamily of ubiquitin‐like post‐translational modifiers. HUB1 exerts its role by conjugating with protein targets. The function of this protein has not been studied in plants. A HUB1 gene, LpHUB1, was identified from serial analysis of gene expression data and cloned from perennial ryegrass. The expression of this gene was reported previously to be elevated in pastures during the summer and by drought stress in climate‐controlled growth chambers. Here, pasture‐type and turf‐type transgenic perennial ryegrass plants overexpressing LpHUB1 showed improved drought tolerance, as evidenced by improved turf quality, maintenance of turgor and increased growth. Additional analyses revealed that the transgenic plants generally displayed higher relative water content, leaf water potential, and chlorophyll content and increased photosynthetic rate when subjected to drought stress. These results suggest HUB1 may play an important role in the tolerance of perennial ryegrass to abiotic stresses.}, number={5}, journal={PLANT BIOTECHNOLOGY JOURNAL}, publisher={Wiley-Blackwell}, author={Patel, Minesh and Milla-Lewis, Susana and Zhang, Wanjun and Templeton, Kerry and Reynolds, William C. and Richardson, Kim and Biswas, Margaret and Zuleta, Maria C. and Dewey, Ralph E. and Qu, Rongda and et al.}, year={2015}, month={Jun}, pages={689–699} }
 @article{patel_dewey_qu_2013, title={Enhancing Agrobacterium tumefaciens-mediated transformation efficiency of perennial ryegrass and rice using heat and high maltose treatments during bacterial infection}, volume={114}, ISSN={["1573-5044"]}, DOI={10.1007/s11240-013-0301-7}, number={1}, journal={PLANT CELL TISSUE AND ORGAN CULTURE}, author={Patel, Minesh and Dewey, Ralph E. and Qu, Rongda}, year={2013}, month={Jul}, pages={19–29} }