@article{xu_knight_boone_saleem_finley_gauthier_ayariga_akinrinlola_pulkoski_britt_et al._2024, title={Influence of Fungicide Application on Rhizosphere Microbiota Structure and Microbial Secreted Enzymes in Diverse Cannabinoid-Rich Hemp Cultivars}, volume={25}, ISSN={["1422-0067"]}, DOI={10.3390/ijms25115892}, abstractNote={Microbes and enzymes play essential roles in soil and plant rhizosphere ecosystem functioning. However, fungicides and plant root secretions may impact the diversity and abundance of microbiota structure and enzymatic activities in the plant rhizosphere. In this study, we analyzed soil samples from the rhizosphere of four cannabinoid-rich hemp (Cannabis sativa) cultivars (Otto II, BaOx, Cherry Citrus, and Wife) subjected to three different treatments (natural infection, fungal inoculation, and fungicide treatment). DNA was extracted from the soil samples, 16S rDNA was sequenced, and data were analyzed for diversity and abundance among different fungicide treatments and hemp cultivars. Fungicide treatment significantly impacted the diversity and abundance of the hemp rhizosphere microbiota structure, and it substantially increased the abundance of the phyla Archaea and Rokubacteria. However, the abundances of the phyla Pseudomonadota and Gemmatimonadetes were substantially decreased in treatments with fungicides compared to those without fungicides in the four hemp cultivars. In addition, the diversity and abundance of the rhizosphere microbiota structure were influenced by hemp cultivars. The influence of Cherry Citrus on the diversity and abundance of the hemp rhizosphere microbiota structure was less compared to the other three hemp cultivars (Otto II, BaOx, and Wife). Moreover, fungicide treatment affected enzymatic activities in the hemp rhizosphere. The application of fungicides significantly decreased enzyme abundance in the rhizosphere of all four hemp cultivars. Enzymes such as dehydrogenase, dioxygenase, hydrolase, transferase, oxidase, carboxylase, and peptidase significantly decreased in all the four hemp rhizosphere treated with fungicides compared to those not treated. These enzymes may be involved in the function of metabolizing organic matter and degrading xenobiotics. The ecological significance of these findings lies in the recognition that fungicides impact enzymes, microbiota structure, and the overall ecosystem within the hemp rhizosphere.}, number={11}, journal={INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES}, author={Xu, Junhuan and Knight, Tyson and Boone, Donchel and Saleem, Muhammad and Finley, Sheree J. and Gauthier, Nicole and Ayariga, Joseph A. and Akinrinlola, Rufus and Pulkoski, Melissa and Britt, Kadie and et al.}, year={2024}, month={Jun} }
 @article{pulkoski_burrack_2023, title={Assessing the impact of piercing-sucking pests on greenhouse-grown industrial hemp (Cannabis sativa L.)}, volume={5}, ISSN={["1938-2936"]}, url={https://doi.org/10.1093/ee/nvad044}, DOI={10.1093/ee/nvad044}, abstractNote={Abstract}, journal={ENVIRONMENTAL ENTOMOLOGY}, author={Pulkoski, Melissa and Burrack, Hannah}, editor={Moreira, XoaquinEditor}, year={2023}, month={May} }
 @article{britt_kuhar_cranshaw_mccullough_taylor_arends_burrack_pulkoski_owens_tolosa_et al._2021, title={Pest Management Needs and Limitations for Corn Earworm (Lepidoptera: Noctuidae), an Emergent Key Pest of Hemp in the United States}, volume={12}, ISSN={["2155-7470"]}, DOI={10.1093/jipm/pmab030}, abstractNote={Abstract}, number={1}, journal={JOURNAL OF INTEGRATED PEST MANAGEMENT}, author={Britt, Kadie E. and Kuhar, Thomas P. and Cranshaw, Whitney and McCullough, Christopher T. and Taylor, Sally V and Arends, Benjamin R. and Burrack, Hannah and Pulkoski, Melissa and Owens, David and Tolosa, Tigist A. and et al.}, year={2021}, month={Sep} }