Works (122)
Updated: June 8th, 2024 05:48
2024 review
Implementation of in situ aerobic cometabolism for groundwater treatment: State of the knowledge and important factors for field operation
[Review of ]. SCIENCE OF THE TOTAL ENVIRONMENT, 925.
author keywords: Aerobic cometabolism; In situ bioremediation; Biodegradation; Groundwater; Dilute plume; Competitive inhibition; Product toxicity; TCE; cDCE; VC; 1,1-DCE; 1,1,1-TCA
open access via doi.org (publisher)
UN Sustainable Development Goal Categories
6. Clean Water and Sanitation
(OpenAlex)
Sources: Web Of Science, NC State University Libraries
Added: May 28, 2024
2023 journal article
Aerobic cometabolic biodegradation of 1,4-dioxane and its associated Co-contaminants
Current Opinion in Environmental Science & Health.
Source: ORCID
Added: January 28, 2024
2023 article
Alcohol-Dependent Cometabolic Degradation of Chlorinated Aliphatic Hydrocarbons and 1,4-Dioxane by Rhodococcus rhodochrous strain ATCC 21198
Bealessio, A. D. D., Chen, W., Krippaehne, K. J. J., Murnane, R. A. A., Hyman, M. R. R., & Semprini, L. (2023, August 2). ENVIRONMENTAL ENGINEERING SCIENCE, Vol. 8.
author keywords: 1; 4-dioxane; activity-based labeling; alcohols; alkanes; chlorinated aliphatic hydrocarbons; cometabolism; esters; short-chain alkane monooxygenase
UN Sustainable Development Goal Categories
6. Clean Water and Sanitation
(OpenAlex)
Sources: Web Of Science, NC State University Libraries
Added: August 21, 2023
2023 journal article
Archaeal communities discovered in the phytotelmata of Nepenthes alata Blco. samples obtained from Mt. Makiling, Philippines as revealed by high-throughput molecular sequencing analysis
International Journal of Agricultural Technology.
Source: ORCID
Added: January 28, 2024
2023 journal article
Diyne inactivators and activity-based fluorescent labeling of phenol hydroxylase in Pseudomonas sp. CF600
FEMS MICROBIOLOGY LETTERS, 370.
author keywords: activity-based labeling; 1; 4-diethynylbenzene; phenol hydroxylase; diyne; mechanism-based inactivation
MeSH headings : Pseudomonas / metabolism; Azides / metabolism; Mixed Function Oxygenases / metabolism; Phenols / metabolism; Phenol / metabolism; Peptides / metabolism
TL;DR:
The results suggest that diverse diynes act as mechanism-based inactivators of phenol hydroxylase in Pseudomonas sp.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
6. Clean Water and Sanitation
(OpenAlex)
Sources: Web Of Science, NC State University Libraries, ORCID
Added: March 27, 2023
2023 article
Identifying the enzyme responsible for initiating aerobic acetylene metabolism in Rhodococcus rhodochrous ATCC 33258
JOURNAL OF BIOLOGICAL CHEMISTRY, Vol. 299, pp. S369–S369.
Sources: Web Of Science, NC State University Libraries
Added: September 11, 2023
2023 journal article
Pilot-scale biofiltration of 1,4-dioxane at drinking water-relevant concentrations
WATER RESEARCH, 231.
author keywords: Biofiltration; Cometabolism; Adsorption; Emerging contaminants
MeSH headings : Drinking Water; Pilot Projects; Water Pollutants, Chemical / analysis; Water Purification / methods; Charcoal / chemistry; Filtration / methods
UN Sustainable Development Goal Categories
6. Clean Water and Sanitation
(OpenAlex)
Sources: Web Of Science, NC State University Libraries, ORCID
Added: March 13, 2023
2022 article
Single-well push-pull tests evaluating isobutane as a primary substrate for promoting in situ cometabolic biotransformation reactions
Rolston, H., Hyman, M., & Semprini, L. (2022, May 12). BIODEGRADATION, Vol. 5.
author keywords: Cometabolism; Push-pull tests; Isobutane; Isobutene; 1; 1-Dichloroethene; 1; 4-Dioxane
MeSH headings : Biodegradation, Environmental; Biotransformation; Butanes; Epoxy Compounds; Groundwater; Water Pollutants, Chemical / metabolism
UN Sustainable Development Goal Categories
6. Clean Water and Sanitation
(OpenAlex)
Sources: Web Of Science, NC State University Libraries, ORCID
Added: May 23, 2022
2021 journal article
Draft Genome Sequences of Four Aerobic Isobutane-Metabolizing Bacteria
MICROBIOLOGY RESOURCE ANNOUNCEMENTS, 10(18).
Ed(s): J. Maresca
TL;DR:
The draft genome sequences of four aerobic gaseous alkane-oxidizing bacteria isolated from soil by enrichment culture using isobutane (2-methylpropane) as the sole carbon and energy source reveal microorganisms with multiple alkane/monooxygenases, including soluble di-iron monooxygenase (SDIMOs), copper-containing mono oxygengenases (CuMMOs), and alkane hydroxylases (AHs).
(via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries, ORCID
Added: June 10, 2021
2021 journal article
Long-term cometabolic transformation of 1,1,1-trichloroethane and 1,4-dioxane by Rhodococcus rhodochrous ATCC 21198 grown on alcohols slowly produced by orthosilicates
JOURNAL OF CONTAMINANT HYDROLOGY, 240.
author keywords: Cometabolism; Orthosilicates; Activity based labeling; Hydrolysis; 1,4-dioxane; 1,1,1-trichloroethane
MeSH headings : Alcohols; Biodegradation, Environmental; Dioxanes; Rhodococcus; Trichloroethanes
TL;DR:
Results suggest that monooxygenase activity was required for the observed cometabolic transformations but not for alcohol utilization, and illustrate the importance of the structure of the SRC when developing passive aerobic cometabolic treatment systems.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
6. Clean Water and Sanitation
(OpenAlex)
Sources: Web Of Science, NC State University Libraries
Added: June 10, 2021
2020 journal article
Co-encapsulation of slow release compounds and Rhodococcus rhodochrous ATCC 21198 in gellan gum beads to promote the long-term aerobic cometabolic transformation of 1,1,1-trichloroethane, cis-1,2-dichloroethene and 1,4-dioxane
Environmental Science: Processes & Impacts.
MeSH headings : Biodegradation, Environmental; Dichloroethylenes; Dioxanes; Polysaccharides, Bacterial; Rhodococcus / chemistry; Trichloroethanes
TL;DR:
The results demonstrate that the co-encapsulation technology can be a passive method for the cometabolic treatment of dilute groundwater plumes.
(via Semantic Scholar)
Source: ORCID
Added: April 28, 2020
2019 journal article
1,4-Dioxane in drinking water: Emerging for forty years and still unregulated
Current Opinion in Environmental Science & Health, 7, 117–125.
UN Sustainable Development Goal Categories
6. Clean Water and Sanitation
(OpenAlex)
Sources: NC State University Libraries, NC State University Libraries, Crossref
Added: February 5, 2019
2019 journal article
Aerobic cometabolism of 1,4-dioxane by isobutane-utilizing microorganisms including Rhodococcus rhodochrous strain 21198 in aquifer microcosms: Experimental and modeling study
SCIENCE OF THE TOTAL ENVIRONMENT, 694.
author keywords: 1,4-dioxane; Cometabolism; Inhibition; Numerical modeling: Groundwater contamination
MeSH headings : Biodegradation, Environmental; Butanes / metabolism; Colorado; Dioxanes / metabolism; Ecosystem; Groundwater / chemistry; Groundwater / microbiology; Rhodococcus / metabolism; Water Microbiology; Water Pollutants, Chemical / analysis; Water Pollutants, Chemical / metabolism
TL;DR:
Results showed that, over time and with sufficient inorganic nutrients, biostimulation of native microorganisms with isobutane was just as effective as bioaugmentation with strain 21198 to achieve 1,4-D transformation in the microcosms.
(via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries, ORCID
Added: December 9, 2019
2019 journal article
Nitrogen Gas Fixation and Conversion to Ammonium Using Microbial Electrolysis Cells
ACS Sustainable Chemistry & Engineering, 7(3), 3511–3519.
author keywords: Nitrogen fixation; Ammonium production; Haber-Bosch; Microbial electrochemical technology; Geobacter
TL;DR:
This study explored the possibility of converting N2 into NH4+ using anaerobic, single-chamber microbial electrolysis cells (MECs) and found that the presence of NH4+, which can inhibit the activity of the nitrogenase enzyme, did not significantly reduce N2 fixation rates.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
7. Affordable and Clean Energy
(Web of Science)
Sources: Web Of Science, NC State University Libraries, Crossref, ORCID
Added: February 5, 2019
2018 chapter
Biodegradation of Ether Pollutants
In Consequences of Microbial Interactions with Hydrocarbons, Oils, and Lipids: Biodegradation and Bioremediation.
Source: ORCID
Added: April 28, 2020
2018 journal article
Concurrent Treatment of 1,4-Dioxane and Chlorinated Aliphatics in a Groundwater Recirculation System Via Aerobic Cometabolism
Groundwater Monitoring & Remediation, 38(3), 53–64.
UN Sustainable Development Goal Categories
6. Clean Water and Sanitation
(OpenAlex)
Sources: Web Of Science, NC State University Libraries
Added: January 31, 2019
2018 journal article
Enrichment with Carbon-13 and Deuterium during Monooxygenase-Mediated Biodegradation of 1,4-Dioxane
Environmental Science & Technology Letters, 5(3), 148–153.
Sources: Web Of Science, NC State University Libraries
Added: January 31, 2019
2016 journal article
Activity-Based Protein Profiling of Ammonia Monooxygenase in Nitrosomonas europaea
Appl. Environ. Microbiol., 82(8), 2270–2279.
MeSH headings : Aerobiosis; Ammonia / metabolism; Chromatography, Liquid; Mass Spectrometry; Nitrosomonas europaea / chemistry; Nitrosomonas europaea / enzymology; Oxidoreductases / analysis; Oxygen / metabolism; Proteome / analysis
TL;DR:
An activity-based protein profiling method for this enzyme using 1,7-octadiyne (17OD) as a probe is described, and peptide fragments from AmoA were the predominant peptides detected in 17OD-treated samples.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
6. Clean Water and Sanitation
(Web of Science; OpenAlex)
Sources: Web Of Science, NC State University Libraries, ORCID
Added: August 6, 2018
2016 chapter
Aerobic Degradation of Gasoline Ether Oxygenates
In Aerobic Utilization of Hydrocarbons, Oils and Lipids.
TL;DR:
This review summarizes the main microorganisms, enzymes, and pathways involved in each of these processes and highlights research areas where there is both clear consensus and areas where results are more ambiguous and likely require further investigation.
(via Semantic Scholar)
Source: ORCID
Added: April 28, 2020
2015 journal article
Isolation and characterization of a 2-methylpropene (isobutylene)-metabolizing bacterium, Mycobacterium sp. ELW1
Applied and Environmental Microbiology, 81, 1966–1976.
Source: NC State University Libraries
Added: February 5, 2019
2015 journal article
Metabolism of 2-Methylpropene (Isobutylene) by the Aerobic Bacterium Mycobacterium sp Strain ELW1
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 81(6), 1966–1976.
MeSH headings : Aerobiosis; Alkenes / metabolism; Carbon / metabolism; Cobalt / metabolism; Coenzymes / metabolism; Culture Media / chemistry; DNA, Bacterial / chemistry; DNA, Bacterial / genetics; DNA, Ribosomal / chemistry; DNA, Ribosomal / genetics; Energy Metabolism; Hydroxybutyrates / metabolism; Molecular Sequence Data; Mycobacterium / isolation & purification; Mycobacterium / metabolism; RNA, Ribosomal, 16S / genetics; Sequence Analysis, DNA
TL;DR:
The results suggest that, like aerobic MTBE- and TBA-metabolizing bacteria, strain ELW1 utilizes a cobalt/cobalamin-dependent mutase to transform 2-hydroxyisobutyrate, and their impact on the understanding of the microbial metabolism of alkenes and ether oxygenates is interpreted.
(via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018
2013 journal article
Oxidation of Cyclic Ethers by Alkane-Grown Mycobacterium vaccae JOB5
Remediation Journal, 23(4), 23–42.
TL;DR:
The cometabolic oxidation of six cyclic ethers by alkane-grown Mycobacterium vaccae JOB5 is investigated and γ-Butyrolactone (γBL) is likely generated during THF oxidation through further oxidation of 2-hydroxytetrahydrofuran, which could have implications for approaches forcyclic ether biodegradation in the environment.
(via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries, ORCID
Added: February 5, 2019
2013 journal article
RNAi silencing of a cytochrome P450 monoxygenase disrupts the ability of a filamentous fungus, Graphium sp., to grow on short-chain gaseous alkanes and ethers
Biodegradation, 25(1), 137–151.
author keywords: Fungi; Alkane oxidation; Tetrahydrofuran; Cometabolism; Cytochrome P450
MeSH headings : Alkanes / metabolism; Biodegradation, Environmental; Cytochrome P-450 Enzyme Inhibitors; Cytochrome P-450 Enzyme System / classification; Cytochrome P-450 Enzyme System / genetics; Cytochrome P-450 Enzyme System / metabolism; Environmental Pollutants / metabolism; Ethers / metabolism; Gases; Gene Expression; Isoenzymes / antagonists & inhibitors; Isoenzymes / classification; Isoenzymes / genetics; Isoenzymes / metabolism; Oxidation-Reduction; Phylogeny; RNA, Small Interfering / genetics; RNA, Small Interfering / metabolism; Saccharomycetales / enzymology; Saccharomycetales / genetics; Sequence Analysis, DNA
TL;DR:
The results of this study suggest that GSPALK1 is the enzyme that catalyzes the initial oxidation of alkanes and ethers but is not involved in the later steps of alkane or ether metabolism.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, NC State University Libraries, ORCID
Added: August 6, 2018
2012 journal article
Biodegradation of gasoline ether oxygenates
Current Opinion in Biotechnology, 24(3), 443–450.
MeSH headings : Biodegradation, Environmental; Butanols / metabolism; Environmental Microbiology; Ether / chemistry; Ether / metabolism; Gasoline; Hydroxybutyrates / metabolism; Methyl Ethers / metabolism; Oxygen / chemistry; Oxygen / metabolism; Propylene Glycols / metabolism; Water Pollutants, Chemical / analysis; Water Pollutants, Chemical / chemistry; Water Pollutants, Chemical / metabolism
TL;DR:
How microbiological and biochemical studies have guided, and have helped refine, molecular and stable isotope-based analytical approaches that are increasingly being used to detect and quantify biodegradation of these compounds in contaminated environments is illustrated.
(via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018
2012 journal article
Preparing Science-Trained Professionals for the Biotechnology Industry: A Ten-Year Perspective on a Professional Science Master’s Program
Journal of Microbiology & Biology Education : JMBE, 13(1), 39–44.
TL;DR:
An overview of the PSM program in Microbial Biotechnology is provided, and some of the lessons that have been learned are shared.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, NC State University Libraries, ORCID
Added: January 31, 2019
2011 journal article
Identification of tertiary butyl alcohol (TBA)-utilizing organisms in BioGAC reactors using 13C-DNA stable isotope probing
Biodegradation, 22(5), 961–972.
author keywords: Tertiary butyl alcohol; Stable isotope probing; Polaromonas; Methylibium
MeSH headings : Bacterial Proteins / genetics; Bacterial Proteins / metabolism; Betaproteobacteria / classification; Betaproteobacteria / genetics; Betaproteobacteria / isolation & purification; Betaproteobacteria / metabolism; Biodegradation, Environmental; Bioreactors / microbiology; Carbon Isotopes / chemistry; DNA, Bacterial / chemistry; DNA, Bacterial / genetics; Fresh Water / microbiology; Isotope Labeling; Molecular Sequence Data; Oxidation-Reduction; Oxygenases / genetics; Oxygenases / metabolism; Phylogeny; Water Pollutants, Chemical / metabolism; tert-Butyl Alcohol / metabolism
TL;DR:
All TBA-utilizing organisms identified were members of the Burkholderiales order of the β-proteobacteria, and one gene encodes a putative phthalate dioxygenase-like enzyme that may be particularly appropriate for determining the potential for TBA oxidation in contaminated environmental samples.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
6. Clean Water and Sanitation
(OpenAlex)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018
2010 journal article
Oxidation of gasoline oxygenates by closely related non-haem-iron alkane hydroxylases in Pseudomonas mendocina KR1 and other n-octane-utilizing Pseudomonas strains
Environmental Microbiology Reports, 2(3), 426–432.
TL;DR:
Results indicate ether oxygenate-degrading activity in alkane-utilizing pseudomonads is consistently observed with close homologues of the GPo1 non-haem-iron alkane hydroxylases but is otherwise not a consistent catalytic feature of these diverse enzymes.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
6. Clean Water and Sanitation
(OpenAlex)
Sources: Web Of Science, NC State University Libraries, ORCID, NC State University Libraries
Added: August 6, 2018
2009 journal article
Effects of gasoline components on MTBE and TBA cometabolism by Mycobacterium austroafricanum JOB5
Biodegradation, 21(4), 525–541.
author keywords: Cometabolism; MTBE; TBA; Gasoline hydrocarbons; Alkanes; BTEX
MeSH headings : Biodegradation, Environmental; Gasoline / analysis; Gasoline / toxicity; Hydrocarbons, Aromatic / pharmacology; Methyl Ethers / metabolism; Mycobacterium / drug effects; Mycobacterium / growth & development; Mycobacterium / metabolism; Oxidation-Reduction; tert-Butyl Alcohol / metabolism
TL;DR:
The effects of acetylene suggest strain JOB5 also has two distinct toluene-oxidizing activities and these results have been discussed in terms of their impact on understanding of MTBE and TBA cometabolism and the enzymes involved in these processes in mycobacteria and other bacteria.
(via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries, ORCID
Added: August 6, 2018
2009 journal article
Helping Educational Reforms To Succeed in a Microbiology Department
Microbe Magazine.
TL;DR:
What makes some reform efforts successful needs to be understood to improve the chances of disseminating these successes to other institutions and to ensure that reforms are to take hold.
(via Semantic Scholar)
Source: ORCID
Added: January 28, 2024
2009 journal article
Helping educational reforms to succeed in a Microbiology Department
Microbe, 4, 219–223.
Source: NC State University Libraries
Added: February 5, 2019
2009 journal article
Isotopic Fractionation of Methyl tert-Butyl Ether Suggests Different Initial Reaction Mechanisms during Aerobic Biodegradation
Environmental Science & Technology, 43(8), 2793–2799.
MeSH headings : Aerobiosis; Bacteria, Aerobic / metabolism; Methyl Ethers / metabolism
TL;DR:
Significantly, delta13C measurements of the tert-butyl alcohol and tert-amyl alcohol products indicated that the secondary metabolites of these groups do not participate in the reaction and confirmed that ether biodegradation by strain K1 involves oxidation of the methoxy (or ethoxy) group.
(via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries, ORCID
Added: August 6, 2018
2009 journal article
Metabolism and Cometabolism of Cyclic Ethers by a Filamentous Fungus, a Graphium sp.
Appl. Environ. Microbiol., 75(17), 5514–5522.
MeSH headings : 4-Butyrolactone / metabolism; Ascomycota / growth & development; Ascomycota / metabolism; Dioxanes / metabolism; Ethers, Cyclic / metabolism; Formaldehyde / metabolism; Formic Acid Esters / metabolism; Furans / metabolism; Metabolic Networks and Pathways; Methanol / metabolism; Models, Biological; Mycelium / growth & development; Mycelium / metabolism; Propane / metabolism
TL;DR:
Observations suggest that THF and n-alkanes may initially be oxidized by the same monooxygenase and that further transformation of THF-derived metabolites involves the activity of one or more alcohol dehydrogenases.
(via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018
2007 journal article
Pathway, inhibition and regulation of methyl tertiary butyl ether oxidation in a filamentous fungus, Graphium sp
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 77(6), 1359–1365.
author keywords: methyl tertiary butyl alcohol; cometabolism; Graphium sp; tertiary butyl alcohol; tertiary butyl formate; alkane monooxygenase
MeSH headings : Ascomycota / metabolism; Biodegradation, Environmental; Methyl Ethers / antagonists & inhibitors; Methyl Ethers / metabolism; Mycelium / metabolism; Oxidation-Reduction; Propane / metabolism; tert-Butyl Alcohol / metabolism
TL;DR:
The results collectively suggest that propane-grown mycelia only have a limited capacity to degrade TBA, and the regulatory effects of MTBE oxidation intermediates proposed for MTBE-degrading organisms such as Mycobacterium austroafricanum are not universally relevant mechanisms for MT BE-degRading organisms.
(via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries, ORCID
Added: August 6, 2018
2007 journal article
The Concept of Readiness in the Academic Department: A Case Study of Undergraduate Education Reform
Innovative Higher Education, 32(1), 3–18.
author keywords: higher education; academic department; change; inquiry-guided learning
UN Sustainable Development Goal Categories
4. Quality Education
(Web of Science; OpenAlex)
Sources: Web Of Science, NC State University Libraries
Added: February 5, 2019
2006 journal article
Propane and n-butane oxidation by Pseudomonas putida GPo1
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 72(1), 950–952.
MeSH headings : Butanes / metabolism; Culture Media; Cytochrome P-450 CYP4A / genetics; Cytochrome P-450 CYP4A / metabolism; Octanes / metabolism; Oxidation-Reduction; Propane / metabolism; Pseudomonas putida / enzymology; Pseudomonas putida / growth & development; Pseudomonas putida / metabolism
TL;DR:
Propane and n-butane inhibit methyl tertiary butyl ether oxidation by n-alkane-grown Pseudomonas putida GPo1 and appear to be oxidized by the same enzyme system used for the oxidation of n-octane.
(via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries, ORCID
Added: August 6, 2018
2004 journal article
Induction of methyl Tertiary butyl ether (MTBE)-oxidizing activity in Mycobacterium vaccae JOB5 by MTBE
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 70(2), 1023–1030.
MeSH headings : Biodegradation, Environmental; Carbohydrate Metabolism; Carbon / metabolism; Carboxylic Acids / metabolism; Culture Media; Gene Expression Regulation, Bacterial; Methyl Ethers / metabolism; Nontuberculous Mycobacteria / growth & development; Nontuberculous Mycobacteria / metabolism; Oxidation-Reduction
TL;DR:
Experiments with acid-grown cells suggested induction of MTBE-oxidizing activity by MTBE is subject to catabolite repression, and the results of this study are discussed in terms of their potential implications towards the understanding of the role of cometabolism in MTBE and TBA biodegradation in gasoline-contaminated environments.
(via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries, ORCID
Added: August 6, 2018
2004 journal article
Induction of methyl tertiary butyl ether (MTBE)-oxidizing activity in Mycobacterium vaccae JOB5 by MTBE
Applied and Environmental Microbiology, 70(2), 1023–1030.
Source: NC State University Libraries
Added: February 5, 2019
2004 journal article
Oxidation of methyl tert-butyl ether by alkane hydroxylase in dicyclopropylketone-induced and n-octane-grown Pseudomonas putida GPo1
Applied and Environmental Microbiology, 70(8), 4544–4550.
MeSH headings : Culture Media; Cyclopropanes / metabolism; Cyclopropanes / pharmacology; Cytochrome P-450 CYP4A / biosynthesis; Cytochrome P-450 CYP4A / genetics; Enzyme Induction; Gene Expression Regulation, Bacterial; Kinetics; Methyl Ethers / metabolism; Octanes / metabolism; Oxidation-Reduction; Pseudomonas putida / enzymology; Pseudomonas putida / genetics; Pseudomonas putida / growth & development
TL;DR:
Kinetic studies with strain GPo1 grown on n-octane or rich medium with DCPK suggest that MTBE-oxidizing activity may have previously gone undetected in n- octane-grown cells because of the unusually high Ks value for MTBE.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
6. Clean Water and Sanitation
(OpenAlex)
Sources: Web Of Science, NC State University Libraries, ORCID
Added: August 6, 2018
2004 book
Teaching and learning through inquiry: A guidebook for institutions and instructors
Sterling, Va.: Stylus Pub.
Source: NC State University Libraries
Added: August 6, 2018
2003 journal article
Acetylene inhibition of trichloroethene and vinyl chloride reductive dechlorination
ENVIRONMENTAL SCIENCE & TECHNOLOGY, 37(14), 3181–3188.
MeSH headings : Acetylene / chemistry; Bacteria, Anaerobic; Biodegradation, Environmental; Carcinogens / chemistry; Carcinogens / metabolism; Chlorine / chemistry; Kinetics; Solvents / chemistry; Trichloroethylene / chemistry; Trichloroethylene / metabolism; Vinyl Chloride / chemistry; Vinyl Chloride / metabolism
TL;DR:
The results suggest that acetylene produced abiotically by reactions of chlorinated ethenes with zero-valent iron could inhibit the biological transformation of VC to ethene and that dehalorespiring organisms are the target of acetylene inhibition.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
6. Clean Water and Sanitation
(OpenAlex)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018
2003 journal article
Characterization of the initial reactions during the cometabolic oxidation of methyl tert-butyl ether by propane-grown Mycobacterium vaccae JOB5
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 69(2), 796–804.
MeSH headings : Biodegradation, Environmental; Culture Media; Kinetics; Methyl Ethers / metabolism; Mycobacterium / growth & development; Mycobacterium / metabolism; Oxidation-Reduction; Propane / metabolism; tert-Butyl Alcohol / metabolism
TL;DR:
The initial steps in the pathway of MTBE oxidation by M. vaccae JOB5 involve two reactions catalyzed by the same monooxygenase (MTBE and TBA oxidation) that are temporally separated by an esterase-catalyzed hydrolysis of TBF to TBA.
(via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries, ORCID
Added: August 6, 2018
2003 journal article
Characterization of the initial reactions during the cometabolic oxidation of methyl tert-butyl ether by propane-grown Mycobacterium vaccae JOB5
Applied and Environmental Microbiology, 69(2), 796–804.
Source: NC State University Libraries
Added: February 5, 2019
2003 journal article
Cometabolism of methyl tertiary butyl ether and gaseous n-alkanes by Pseudomonas mendocina KR-1 grown on C(5) to C(8) n-alkanes
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 69(12), 7385–7394.
MeSH headings : Alkanes / metabolism; Culture Media; Kinetics; Methyl Ethers / metabolism; Oxidation-Reduction; Pseudomonas mendocina / growth & development; Pseudomonas mendocina / metabolism; Time Factors
TL;DR:
Cultures grown on C5 to C8n-alkanes exhibited up to twofold-higher levels of growth in the presence of propane or n-butane, whereas no growth stimulation was observed with methane, ethane, MTBE, TBA, or formaldehyde.
(via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries, ORCID
Added: August 6, 2018
2003 journal article
Cometabolism of methyl tertiary butyl ether and gaseous n-alkanes by Pseudomonas mendocina KR-1 grown on C5 to C8 n-alkanes
Applied and Environmental Microbiology, 69(12), 7385–7394.
Source: NC State University Libraries
Added: February 5, 2019
2002 journal article
Cooxidation of naphthalene and other polycyclic aromatic hydrocarbons by the nitrifying bacterium, Nitrosomonas europaea
Biodegradation (Dordrecht), 13(6), 373–381.
Source: NC State University Libraries
Added: August 6, 2018
2002 journal article
Cooxidation of naphthalene and other polycyclic aromatic hydrocarbons by the nitrifying bacterium, Nitrosomonas europaea.
Biodegradation.
MeSH headings : Acetylene / pharmacology; Ammonia / metabolism; Binding, Competitive; Biodegradation, Environmental; Kinetics; Naphthalenes / metabolism; Naphthalenes / pharmacology; Nitrosomonas europaea / metabolism; Oxidoreductases / antagonists & inhibitors; Oxidoreductases / metabolism; Oxygen / metabolism; Polycyclic Aromatic Hydrocarbons / metabolism; Soil Microbiology
TL;DR:
NH4+- and N2H4-dependent O2 uptake measurement demonstrated irreversible inhibitory effects of the naphthalene and subsequent oxidation products on AMO and HAO activity.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
6. Clean Water and Sanitation
(Web of Science)
Source: ORCID
Added: April 28, 2020
2001 journal article
Hydrolysis of tert-Butyl Methyl Ether (MTBE) in Dilute Aqueous Acid
Environmental Science & Technology, 35(19), 3954–3961.
MeSH headings : Air Pollutants / chemistry; Hydrogen-Ion Concentration; Hydrolysis; Kinetics; Methyl Ethers / chemistry
TL;DR:
The results demonstrate that MTBE is sensitive to acid-catalyzed hydrolysis reaction that generates tert-butyl alcohol (TBA) and methanol as products.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
6. Clean Water and Sanitation
(OpenAlex)
Sources: Web Of Science, NC State University Libraries, ORCID
Added: August 6, 2018
2001 journal article
Molecular and cellular fundamentals of aerobic cometabolism of trichloroethylene
Biodegradation (Dordrecht), 12(2), 81–103.
Source: NC State University Libraries
Added: August 6, 2018
2001 journal article
Molecular and cellular fundamentals of aerobic cometabolism of trichloroethylene.
Biodegradation.
author keywords: bioremediation; cometabolism; cooxidation; methane monooxygenase; toluene monooxygenase; trichloroethylene
MeSH headings : Aerobiosis; Bacteria / metabolism; Biodegradation, Environmental; Enzymes / genetics; Enzymes / metabolism; Gene Expression Regulation; Genetic Engineering; Mixed Function Oxygenases / metabolism; Oxidoreductases / metabolism; Oxygenases / chemistry; Oxygenases / metabolism; Solvents / metabolism; Trichloroethylene / metabolism
TL;DR:
This review has examined the aerobic cometabolism of chlorinated solvents, with a particular emphasis on the cometabolisms of trichloroethylene.
(via Semantic Scholar)
Source: ORCID
Added: April 28, 2020
1999 journal article
Anaerobic Biotransformation of Trichlorofluoroethene in Groundwater Microcosms
Environmental Science & Technology, 33(12), 2040–2045.
TL;DR:
The biological reduction of trichlorofluoroethene (TCFE) was investigated in anaerobic groundwater microcosms and close similarities between the time course and kinetics of TCFE were observed.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
6. Clean Water and Sanitation
(OpenAlex)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018
1999 journal article
Cometabolism of chlorinated solvents by nitrifying bacteria: kinetics, substrate interactions, toxicity effects, and bacterial response
Biotechnology and Bioengineering.
TL;DR:
A method for estimating protein yield, the relationship between the transformation capacity model and the IIR model, and a condition necessary for sustainable cometabolic treatment of inactivating substrates are presented.
(via Semantic Scholar)
Source: ORCID
Added: April 28, 2020
1999 journal article
Inactivation of toluene 2-monooxygenase in Burkholderia cepacia G4 by alkynes
Applied and Environmental Microbiology, 65(2), 632–639.
Source: NC State University Libraries
Added: August 6, 2018
1999 journal article
Inactivation of toluene 2-monooxygenase in Burkholderia cepacia G4 by alkynes.
Applied and Environmental Microbiology. https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/9925593/?tool=EBI
Source: ORCID
Added: April 28, 2020
1998 journal article
Spatial Variability in In Situ Aerobic Respiration and Denitrification Rates in a Petroleum-Contaminated Aquifer
Groundwater, 36(6), 924–937.
UN Sustainable Development Goal Categories
6. Clean Water and Sanitation
(OpenAlex)
Sources: Web Of Science, NC State University Libraries
Added: February 5, 2019
1997 journal article
Cometabolism of chlorinated solvents by nitrifying bacteria: kinetics, substrate interactions, toxicity effects, and bacterial response
Biotechnology and Bioengineering, 54(6), 520–534.
Sources: NC State University Libraries, NC State University Libraries, ORCID
Added: February 5, 2019
1997 journal article
Metabolism of Diethyl Ether and Cometabolism of Methyl tert-Butyl Ether by a Filamentous Fungus, a Graphium sp
Applied and Environmental Microbiology, 63(8), 3059–3067. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1389222/
Source: NC State University Libraries
Added: February 5, 2019
1997 journal article
Metabolism of Diethyl Ether and Cometabolism of Methyl tert-Butyl Ether by a Filamentous Fungus, a Graphium sp.
Applied and Environmental Microbiology. https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/16535667/?tool=EBI
Source: ORCID
Added: April 28, 2020
1997 journal article
Regulation of the Synthesis and Activity of Ammonia Monooxygenase in Nitrosomonas europaea by Altering pH To Affect NH(inf3) Availability.
Applied and Environmental Microbiology. https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/16535741/?tool=EBI
Source: ORCID
Added: April 28, 2020
1997 journal article
Regulation of the synthesis and activity of ammonia monooxygenase in Nitrosomonas europaea by altering pH to affect NH3 availability
Applied and Environmental Microbiology, 63, 4588–4592.
Source: NC State University Libraries
Added: February 5, 2019
1997 journal article
Single-Well, “Push-Pull” Test for In Situ Determination of Microbial Activities
Groundwater, 35(4), 619–631.
Sources: Web Of Science, NC State University Libraries
Added: February 5, 2019
1996 journal article
Inhibition of Growth of a Graphium sp. on Gaseous n-Alkanes by Gaseous n-Alkynes and n-Alkenes
Applied and Environmental Microbiology, 62(6), 2198–2200. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1388884/
Source: NC State University Libraries
Added: February 5, 2019
1996 journal article
Inhibition of Growth of a Graphium sp. on Gaseous n-Alkanes by Gaseous n-Alkynes and n-Alkenes.
Applied and Environmental Microbiology. https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/16535346/?tool=EBI
Source: ORCID
Added: January 28, 2024
1995 journal article
A cometabilic kinetics model incorporating enzyme inhbition, inactivation, and recovery: I. Model development, analysis, and testing
Biotechnology and Bioengineering, 46(3), 218–231.
author keywords: KINETICS MODEL; TRICHLOROETHYLENE; AMMONIA OXIDATION; COMETABOLISM
TL;DR:
A conceptual and mathematical model of cometabolic enzyme kinetics is described and shows that growth substraate and nongrowth substrate oxidation rates are related by a dimensionless constant.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
6. Clean Water and Sanitation
(OpenAlex)
Sources: Web Of Science, NC State University Libraries, ORCID
Added: February 5, 2019
1995 journal article
A cometabolic kinetics model incoroporating enzyme inhibition, inactivation, and recovery: II. Trichloroethylene degradaation experiments
Biotechnology and Bioengineering, 46(3), 232–245.
TL;DR:
Analysis of data and comparison of model results showed the inhibition/inactivation/recovery concept to provide a reasonable basis for understandign the effects of TCE on AMO function and bacterial response, and questions remain regarding the factores controlling recovery and its role in the long term.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
6. Clean Water and Sanitation
(OpenAlex)
Sources: NC State University Libraries, NC State University Libraries, ORCID
Added: February 5, 2019
1995 journal article
Effects of ammonia on the de novo synthesis of polypeptides in cells of Nitrosomonas europaea denied ammonia as an energy source
Journal of Bacteriology, 177(17), 4974–4979.
Source: NC State University Libraries
Added: February 5, 2019
1995 journal article
Effects of ammonia on the de novo synthesis of polypeptides in cells of Nitrosomonas europaea denied ammonia as an energy source.
Journal of Bacteriology.
MeSH headings : Acetylene / metabolism; Ammonia / metabolism; Bacterial Proteins / biosynthesis; Carbon Dioxide / metabolism; Energy Metabolism; Gene Expression Regulation, Bacterial; Hydrogen-Ion Concentration; Hydroxylamine; Hydroxylamines / metabolism; Nitrosomonas / metabolism; Oxidoreductases / metabolism; Peptide Biosynthesis
TL;DR:
Ammonia has a regulatory effect on the synthesis of a subunit of AMO in addition to providing nitrogen for protein synthesis, and is concluded to cause a saturable response in cells using NH4+ as an energy source.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
7. Affordable and Clean Energy
(OpenAlex)
Source: ORCID
Added: April 28, 2020
1995 journal article
Inhibition, Inactivation, and Recovery of Ammonia-Oxidizing Activity in Cometabolism of Trichloroethylene by Nitrosomonas europaea
Applied and Environmental Microbiology, 61(4), 1480–1487.
Source: NC State University Libraries
Added: February 5, 2019
1995 journal article
Inhibition, Inactivation, and Recovery of Ammonia-Oxidizing Activity in Cometabolism of Trichloroethylene by Nitrosomonas europaea.
Applied and Environmental Microbiology. https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/16534997/?tool=EBI
Source: ORCID
Added: April 28, 2020
1995 journal article
Roles of bovine serum albumin and copper in the assay and stability of ammonia monooxygenase activity in vitro
Journal of Bacteriology, 177(17), 4908–4913.
Source: NC State University Libraries
Added: February 5, 2019
1995 journal article
Roles of bovine serum albumin and copper in the assay and stability of ammonia monooxygenase activity in vitro.
Journal of Bacteriology.
MeSH headings : Adsorption; Ammonia / metabolism; Animals; Cattle; Cell Extracts; Copper / pharmacology; Enzyme Stability / drug effects; Fatty Acids, Monounsaturated / pharmacology; Lactoglobulins / pharmacology; Lipolysis / drug effects; Nitrosomonas / enzymology; Oxidoreductases / analysis; Oxidoreductases / drug effects; Oxygen Consumption; Serum Albumin / pharmacology; Serum Albumin, Bovine / pharmacology; Species Specificity; Specimen Handling; Swine
TL;DR:
The role of BSA in the assay of ammonia-oxidizing activity apparently is to absorb endogenous free fatty acids which are present in the extracts, because both additives inhibited the increase in concentrations of free palmitoleic acid in aging extracts.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
6. Clean Water and Sanitation
(OpenAlex)
Source: ORCID
Added: April 28, 2020
1994 journal article
Oxidation of methyl fluoride and dimethyl ether by ammonia monooxygenase in Nitrosomonas europaea
Applied and Environmental Microbiology, 60(8), 3033–3035.
Source: NC State University Libraries
Added: February 5, 2019
1994 journal article
Oxidation of methyl fluoride and dimethyl ether by ammonia monooxygenase in Nitrosomonas europaea.
Applied and Environmental Microbiology. https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/8085841/?tool=EBI
Source: ORCID
Added: April 28, 2020
1993 journal article
An electrophoretic study of the thermal- and reductant-dependent aggregation of the 27 kDa component of ammonia monooxygenase from Nitrosomonas europaea
Electrophoresis, 14(7), 619–627.
Source: NC State University Libraries
Added: February 5, 2019
1993 journal article
An electrophoretic study of the thermal- and reductant-dependent aggregation of the 27 kDa component of ammonia monooxygenase from Nitrosomonas europaea.
Electrophoresis.
MeSH headings : Carbon Dioxide / metabolism; Carbon Radioisotopes; Chromatography, Gel; Electrophoresis, Polyacrylamide Gel / methods; Hot Temperature; Hydrogen-Ion Concentration; Kinetics; Macromolecular Substances; Mercaptoethanol / pharmacology; Molecular Weight; Nitrosomonas / enzymology; Oxidoreductases / chemistry; Sodium Dodecyl Sulfate / pharmacology; Trichloroethylene / metabolism
TL;DR:
It is concluded that strongly hydrophobic amino acid sequences present in ammonia monooxygenase are responsible for the aggregation phenomenon.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
6. Clean Water and Sanitation
(OpenAlex)
Source: ORCID
Added: April 28, 2020
1993 journal article
In vitro activation of ammonia monooxygenase from Nitrosomonas europaea by copper.
Journal of Bacteriology.
MeSH headings : Acetylene / pharmacology; Ammonia / metabolism; Cell-Free System; Copper / pharmacology; Enzyme Activation; Enzyme Stability / drug effects; Ethane / metabolism; Hydrazines / pharmacology; Metals / pharmacology; Nitrites / metabolism; Nitrosomonas / enzymology; Oxidation-Reduction; Oxygen Consumption; Oxygenases / drug effects; Oxygenases / metabolism; Serum Albumin / pharmacology
TL;DR:
It is proposed that loss of AMO activity upon lysis of N. europaea results from the loss of copper from AMO, generating a catalytically inactive, yet stable and activable, form of the enzyme.
(via Semantic Scholar)
Source: ORCID
Added: April 28, 2020
1993 journal article
In vitro activation of ammonia monooxygenase from Nitrosomonas europaea by copper.
Journal of Bacteriology, 175(7), 1971–1980. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC204278/
Source: NC State University Libraries
Added: February 5, 2019
1993 journal article
Inhibition of Ammonia Oxidation in Nitrosomonas europaea by Sulfur Compounds: Thioethers Are Oxidized to Sulfoxides by Ammonia Monooxygenase
Applied and Environmental Microbiology, 59(11), 3718–3727.
Source: NC State University Libraries
Added: February 5, 2019
1993 journal article
Inhibition of Ammonia Oxidation in Nitrosomonas europaea by Sulfur Compounds: Thioethers Are Oxidized to Sulfoxides by Ammonia Monooxygenase.
Applied and Environmental Microbiology. https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/16349086/?tool=EBI
Source: ORCID
Added: April 28, 2020
1993 journal article
Mechanism-Based Inactivation of Ammonia Monooxygenase in Nitrosomonas europaea by Allylsulfide
Applied and Environmental Microbiology, 59(11), 3728–3735.
Source: NC State University Libraries
Added: February 5, 2019
1993 journal article
Mechanism-Based Inactivation of Ammonia Monooxygenase in Nitrosomonas europaea by Allylsulfide.
Applied and Environmental Microbiology. https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/16349087/?tool=EBI
Source: ORCID
Added: April 28, 2020
1992 journal article
14C2H2- and 14CO2-labeling studies of the de novo synthesis of polypeptides by Nitrosomonas europaea during recovery from acetylene and light inactivation of ammonia monooxygenase
The Journal of Biological Chemistry, 267(3), 1534–1545.
Source: NC State University Libraries
Added: February 5, 2019
1992 journal article
14C2H2- and 14CO2-labeling studies of the de novo synthesis of polypeptides by Nitrosomonas europaea during recovery from acetylene and light inactivation of ammonia monooxygenase.
The Journal of Biological Chemistry.
Source: ORCID
Added: April 28, 2020
1992 journal article
Acetylene inhibition of Azotobacter vinelandii hydrogenase: acetylene binds tightly to the large subunit.
Biochemistry.
MeSH headings : Acetylene / chemistry; Acetylene / pharmacology; Azotobacter vinelandii / chemistry; Azotobacter vinelandii / drug effects; Azotobacter vinelandii / enzymology; Binding, Competitive; Carbon Radioisotopes; Hydrogenase / antagonists & inhibitors; Hydrogenase / chemistry; Hydrogenase / isolation & purification; Kinetics; Protein Binding; Protein Conformation; Substrate Specificity
TL;DR:
The result suggests that C2H2, and by analogy H2, binds to and is activated by the large subunit of this dimeric hydrogenase, which is remarkably specific for C 2H2: propyne, butyne, and ethylene are not inhibitors.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
6. Clean Water and Sanitation
(OpenAlex)
Source: ORCID
Added: April 28, 2020
1992 journal article
C2H2 Inhibition of Azotobacter vinelandii hydrogenase: C2H2 binds tightly to the large subunit
Biochemistry, 31, 3158–3165.
Source: NC State University Libraries
Added: February 5, 2019
1992 journal article
Cometabolic degradation of chlorinated alkenes by alkene monooxygenase in a propylene-grown Xanthobacter strain
Applied and Environmental Microbiology, 58(9), 3038–3046.
Source: NC State University Libraries
Added: February 5, 2019
1992 journal article
Cometabolic degradation of chlorinated alkenes by alkene monooxygenase in a propylene-grown Xanthobacter strain.
Applied and Environmental Microbiology. https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/1444418/?tool=EBI
Source: ORCID
Added: April 28, 2020
1992 journal article
Kinetic and spectroscopic analysis of the inactivating effects of nitric oxide on the individual components of Azotobacter vinelandii nitrogenase
Biochemistry, 31(11), 2947–2955.
Source: NC State University Libraries
Added: February 5, 2019
1992 journal article
Kinetic and spectroscopic analysis of the inactivating effects of nitric oxide on the individual components of Azotobacter vinelandii nitrogenase.
Biochemistry.
MeSH headings : Adenosine Diphosphate / pharmacology; Adenosine Triphosphate / pharmacology; Azotobacter vinelandii / enzymology; Electron Spin Resonance Spectroscopy; Iron / metabolism; Kinetics; Macromolecular Substances; Nitric Oxide / pharmacology; Nitrogenase / antagonists & inhibitors; Nitrogenase / metabolism; Spectrophotometry
TL;DR:
The effects of nitric oxide (NO) on the individual components of Azotobacter vinelandii nitrogenase have been examined by kinetic and spectroscopic methods and revealed that the effect of NO on Av2 involves oxidation of the [4Fe-4S] center.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
6. Clean Water and Sanitation
(OpenAlex)
Source: ORCID
Added: April 28, 2020
1991 journal article
Factors Limiting Aliphatic Chlorocarbon Degradation by Nitrosomonas europaea: Cometabolic Inactivation of Ammonia Monooxygenase and Substrate Specificity
Applied and Environmental Microbiology, 57(10), 2986–2994.
Source: NC State University Libraries
Added: February 5, 2019
1991 journal article
Factors Limiting Aliphatic Chlorocarbon Degradation by Nitrosomonas europaea: Cometabolic Inactivation of Ammonia Monooxygenase and Substrate Specificity.
Applied and Environmental Microbiology. https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/16348568/?tool=EBI
Source: ORCID
Added: April 28, 2020
1991 journal article
Kinetic analysis of the interaction of nitric oxide with the membrane-associated, nickel and iron-sulfur-containing hydrogenase from Azotobacter vinelandii.
Biochimica Et Biophysica Acta.
author keywords: HYDROGENASE; NITRIC OXIDE INHIBITION; (AZOTOBACTER-VINELANDII)
MeSH headings : Azotobacter / enzymology; Cell Membrane / enzymology; Hydrogenase / antagonists & inhibitors; Iron-Sulfur Proteins / metabolism; Kinetics; Models, Theoretical; Nickel / analysis; Nitric Oxide / metabolism; Nitric Oxide / pharmacology
TL;DR:
The effects of nitric oxide on the membrane-associated form of the nickel and iron-sulfur-containing hydrogenase from Azotobacter vinelandii have been investigated andKinetic evidence is presented which is compatible with an interaction between NO and a redox-active component other than the H2-activating site on the enzyme.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
6. Clean Water and Sanitation
(OpenAlex)
Source: ORCID
Added: April 28, 2020
1991 journal article
Kinetic analysis of the interaction of nitric oxide with the nickel and iron-sulfur-containing membrane-bound hydrogenase from Azotobacter vinelandii
Biochimica Biophysica Acta, 1076, 167–174.
Source: NC State University Libraries
Added: February 5, 2019
1990 journal article
Biodegradation of Halogenated Hydrocarbon Fumigants by Nitrifying Bacteria
Applied and Environmental Microbiology, 56(8), 2568–2571.
Source: NC State University Libraries
Added: February 5, 2019
1990 journal article
Biodegradation of Halogenated Hydrocarbon Fumigants by Nitrifying Bacteria.
Applied and Environmental Microbiology. https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/16348264/?tool=EBI
Source: ORCID
Added: January 28, 2024
1990 journal article
Inhibition of ammonia monooxygenase in Nitrosomonas europaea by carbon disulfide
Journal of Bacteriology, 172(9), 4775–4782.
Source: NC State University Libraries
Added: February 5, 2019
1990 journal article
Inhibition of ammonia monooxygenase in Nitrosomonas europaea by carbon disulfide.
Journal of Bacteriology.
MeSH headings : Acetylene / pharmacology; Anaerobiosis; Carbon Disulfide / pharmacology; Kinetics; Nitrosomonas / enzymology; Oxidoreductases / antagonists & inhibitors; Oxygen Consumption; Thiourea / pharmacology
TL;DR:
Results suggest that ammonia monooxygenase (AMO) is the site of action of CS2, and it is proposed that CS2 may act on AMO by reversibly reacting with a suitable nucleophilic amino acid in close proximity to the active site copper.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
6. Clean Water and Sanitation
(OpenAlex)
Source: ORCID
Added: April 28, 2020
1990 journal article
Oxidation of monohalogenated ethanes and n-chlorinated alkanes by whole cells of Nitrosomonas europaea
Journal of Bacteriology, 172(9), 5368–5373.
Source: NC State University Libraries
Added: February 5, 2019
1990 journal article
Oxidation of monohalogenated ethanes and n-chlorinated alkanes by whole cells of Nitrosomonas europaea.
Journal of Bacteriology.
MeSH headings : Alkanes / metabolism; Ethane / analogs & derivatives; Ethane / metabolism; Hydrocarbons, Chlorinated / metabolism; Hydrocarbons, Halogenated / metabolism; Kinetics; Nitrosomonas / metabolism; Oxidation-Reduction; Structure-Activity Relationship
TL;DR:
The concentration profiles for halocarbon oxidation by ammonia monooxygenase showed apparent substrate inhibition when ammonia was used as the reductant source and no substrate inhibition was observed, suggesting that the inhibition resulted from reductants limitation.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
6. Clean Water and Sanitation
(OpenAlex)
Source: ORCID
Added: April 28, 2020
1990 journal article
The small-scale production of [U-14C] acetylene from Ba14C-CO3: Application to labelling of ammonia monooxygenase in autotrophic nitrifying bacteria
Analytical Biochemistry, 190, 348–353.
Source: NC State University Libraries
Added: February 5, 2019
1990 journal article
The small-scale production of [U-14C]acetylene from Ba14CO3: application to labeling of ammonia monooxygenase in autotrophic nitrifying bacteria.
Analytical Biochemistry.
MeSH headings : Acetylene / metabolism; Barium / metabolism; Bradyrhizobiaceae / enzymology; Carbonates; Molecular Weight; Oxidoreductases / metabolism; Uranium
TL;DR:
The results presented demonstrate the effect of Ba:BaCO3 ratio on the concentrations of various gases released during the hydrolysis reaction and quantify the selectivity of the DMSO-trapping process for each gas.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
14. Life Below Water
(OpenAlex)
Source: ORCID
Added: April 28, 2020
1989 journal article
Carbonyl sulfide inhibition of CO dehydrogenase from Rhodospirillum rubrum
Biochemistry, 28(17), 6821–6826.
MeSH headings : Aldehyde Oxidoreductases / antagonists & inhibitors; Binding, Competitive; Kinetics; Multienzyme Complexes; Paraquat / pharmacology; Rhodospirillum rubrum / enzymology; Sulfur Oxides / pharmacology
TL;DR:
The ability of CO, CO2, COS, and related CO/CO2 analogues to reverse cyanide inhibition of CO dehydrogenase is demonstrated and is compatible with a ping-pong mechanism for CO oxidation and COS inhibition.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
6. Clean Water and Sanitation
(OpenAlex)
Sources: Web Of Science, NC State University Libraries, ORCID
Added: February 5, 2019
1989 journal article
Nickel-specific, slow-binding inhibition of carbon monoxide dehydrogenase from Rhodospirillum rubrum by cyanide
Biochemistry, 28(12), 4973–4979.
MeSH headings : Aldehyde Oxidoreductases / antagonists & inhibitors; Binding Sites / drug effects; Binding, Competitive; Carbon Monoxide / pharmacology; Cyanides / pharmacology; Drug Synergism; Enzyme Activation / drug effects; Kinetics; Multienzyme Complexes; Nickel / pharmacology; Potassium Cyanide / pharmacology; Rhodospirillum rubrum / enzymology
TL;DR:
It is proposed that cyanide is a slow-binding, active-site-directed, nickel-specific, reversible inhibitor of CO dehydrogenase by being an analogue of CO and by binding through enzyme-bound nickel.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
6. Clean Water and Sanitation
(OpenAlex)
Sources: Web Of Science, NC State University Libraries, ORCID
Added: February 5, 2019
1988 journal article
Acetylene inhibition of metalloenzymes
Analytical Biochemistry.
MeSH headings : Acetylene / toxicity; Enzyme Inhibitors; Metalloproteins
Source: ORCID
Added: April 28, 2020
1988 journal article
Acetylene inhibition of metalloenzymes
Analytical Biochemistry, 173(2), 207–220.
Source: NC State University Libraries
Added: February 5, 2019
1988 journal article
Aerobic, inactive forms of Azotobacter vinelandii hydrogenase: activation kinetics and insensitivity to C2H2 inhibition
Biochimica Et Biophysica Acta, 957(1), 91–96.
Source: NC State University Libraries
Added: February 5, 2019
1988 journal article
Aerobic, inactive forms of Azotobacter vinelandii hydrogenase: activation kinetics and insensitivity to C2H2 inhibition.
Biochimica Et Biophysica Acta.
MeSH headings : Acetylene / pharmacology; Aerobiosis; Azotobacter / enzymology; Cell Membrane / enzymology; Enzyme Activation; Hydrogen; Hydrogenase / antagonists & inhibitors; Kinetics; Oxidation-Reduction
TL;DR:
Aerobically prepared, inactive hydrogenase was insensitive to acetylene inhibition, but could be rendered acetylene-sensitive by reduction with dithionite, indicating that acetylene inhibited hydrogenase requires catalytically active enzyme.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
6. Clean Water and Sanitation
(OpenAlex)
Source: ORCID
Added: April 28, 2020
1988 journal article
Interaction of Ammonia Monooxygenase from Nitrosomonas europaea with Alkanes, Alkenes, and Alkynes
Applied and Environmental Microbiology, 54(12), 3187–3190. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC204451/
Source: NC State University Libraries
Added: February 5, 2019
1988 journal article
Interaction of Ammonia Monooxygenase from Nitrosomonas europaea with Alkanes, Alkenes, and Alkynes.
Applied and Environmental Microbiology. https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/16347810/?tool=EBI
Source: ORCID
Added: April 28, 2020
1988 journal article
Reversible and irreversible effects of nitric oxide on the soluble hydrogenase from Alcaligenes eutrophus H16
Biochemical Journal, 254(2), 469–475.
MeSH headings : Alcaligenes / enzymology; Dihydrolipoamide Dehydrogenase / antagonists & inhibitors; Enzyme Activation / drug effects; Hydrogen / metabolism; Hydrogenase / antagonists & inhibitors; Kinetics; NAD / pharmacology; Nitric Oxide / pharmacology; Oxidation-Reduction
TL;DR:
The effects of NO on the H2-oxidizing and diaphorase activities of the soluble hydrogenase from Alcaligenes eutrophus H16 were investigated and were interpreted in terms of a complex inhibition that displays elements of a reversible slow-binding inhibition of H1-oxIDizing activity and an irreversible effect on H2/NAD+ activity.
(via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries, ORCID
Added: February 5, 2019
1988 journal article
Role of hydrogen in the activation and regulation of hydrogen oxidation by the soluble hydrogenase from Alcaligenes eutrophus H16
Biochemical Journal, 254(2), 463–468. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1135100/
Source: NC State University Libraries
Added: February 5, 2019
1988 journal article
Role of hydrogen in the activation and regulation of hydrogen oxidation by the soluble hydrogenase from Alcaligenes eutrophus H16.
The Biochemical Journal.
MeSH headings : Alcaligenes / enzymology; Dihydrolipoamide Dehydrogenase / metabolism; Enzyme Activation / drug effects; Hydrogen / metabolism; Hydrogenase / metabolism; Kinetics; NAD / pharmacology; Oxidation-Reduction; Thiosulfates / pharmacology
TL;DR:
It is suggested that H2 plays an important role in regulating the level of H2-oxidizing activity in this soluble hydrogenase and whether H2 oxidation was assayed by H1-dependent Methylene Blue or NAD+ reduction.
(via Semantic Scholar)
Source: ORCID
Added: April 28, 2020
1987 journal article
Acetylene is an active-site-directed, slow-binding, reversible inhibitor of Azotobacter vinelandii hydrogenase
Biochemistry, 26(20), 6447–6454.
TL;DR:
Findings suggest that acetylene is an active-site-directed, slow-binding, reversible inhibitor of some membrane-bound hydrogenases from aerobic bacteria.
(via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: February 5, 2019
1987 journal article
Quantification and removal of some contaminating gases from acetylene used to study gas-utilizing enzymes and microorganisms
Applied and Environmental Microbiology, 53(2), 298–303.
Source: NC State University Libraries
Added: February 5, 2019
1987 journal article
Quantification and removal of some contaminating gases from acetylene used to study gas-utilizing enzymes and microorganisms.
Applied and Environmental Microbiology. https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/16347278/?tool=EBI
Source: ORCID
Added: April 28, 2020
1985 journal article
A kinetic study of benzene oxidation to phenol by whole cells of Nitrosomonas europaea and evidence for the further oxidation of phenol to hydroquinone
Archives of Microbiology, 143(3), 302–306.
TL;DR:
Phenol was found to be further hydroxylated to yield hydroquinone, and was sensitive to the ammonia monooxygenase inhibitor allylthiourea, implying that at least 88% of the Hydroxylation is para-directed.
(via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: February 5, 2019
1985 journal article
Suicidal inactivation and labelling of ammonia mono-oxygenase by acetylene.
The Biochemical Journal.
MeSH headings : Acetylene / analogs & derivatives; Acetylene / pharmacology; Ammonia / metabolism; Cell Membrane / metabolism; Electrophoresis, Polyacrylamide Gel; Kinetics; Nitrosomonas / drug effects; Nitrosomonas / enzymology; Oxidoreductases / antagonists & inhibitors; Oxygen / metabolism; Peptide Fragments / analysis
TL;DR:
It is concluded that acetylene is a suicide substrate for the mono-oxygenase, and the labelling experiment provides the first identification of a constituent polypeptide of ammonia mono-oxidizing enzyme in Nitrosomonas europaea.
(via Semantic Scholar)
Source: ORCID
Added: April 28, 2020
1985 journal article
Suicidal inactivation and labelling of ammonia mono-oxygenase by acetylene.
Biochemical Journal, 227(3), 719–725. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1144898/
Source: NC State University Libraries
Added: February 5, 2019
1984 chapter
Bromocarbon oxidation by Nitrosomonas europaea
In Microbial Growth on C1 Compounds.
Source: ORCID
Added: April 28, 2020
1984 journal article
Ethylene oxidation by Nitrosomonas europaea
Archives of Microbiology, 137(2), 155–158.
TL;DR:
The data suggest that at high concentrations of ethylene the rate of oxidation is limited by the rate at which reducing power can be supplied to the monooxygenase, not by an intrinsic Vmax.
(via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: February 5, 2019
1983 journal article
Methane oxidation by Nitrosomonas europaea
The Biochemical Journal, 212(1), 31–37.
Source: NC State University Libraries
Added: February 5, 2019
1983 journal article
Methane oxidation by Nitrosomonas europaea.
The Biochemical Journal.
MeSH headings : Ammonium Sulfate / metabolism; Kinetics; Methane / metabolism; Methanol / metabolism; Nitrosomonas / metabolism; Oxidation-Reduction; Oxidoreductases / metabolism; Oxygen Consumption
TL;DR:
It is concluded that methane is an alternative substrate for the NH3-oxidizing enzyme (ammonia mono-oxygenase), albeit with a much lower affinity than for methane mono- oxygengenase of methanotrophs.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
6. Clean Water and Sanitation
(OpenAlex)
Source: ORCID
Added: April 28, 2020
journal article
Nickel-specific, slow-binding inhibition of carbon monoxide dehydrogenase from Rhodospirillum rubrum by cyanide
Ensign, S. A., Hyman, M. R., & Ludden, P. W. Biochemistry, 28, 4973–4979.
Source: NC State University Libraries
Added: February 5, 2019
Employment
Updated: April 27th, 2020 10:54
2011 - present
North Carolina State University Raleigh, NC, US
Professor
Plant and Microbial Biology
Education
Updated: April 27th, 2020 11:01
Oregon State University Corvallis, OR, US
MBA
Business
1981 - 1985
University of Bristol Bristol, Bristol, GB
PhD
Biochemistry
1977 - 1980
University College London London, London, GB
BS
Botany & Microbiology
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