@article{steffey_risselada_scharf_buote_zamprogno_winter_griffon_2023, title={A narrative review of the impact of work hours and insufficient rest on job performance}, ISSN={["1532-950X"]}, DOI={10.1111/vsu.13943}, abstractNote={AbstractObjectiveThis review discusses the scientific evidence regarding effects of insufficient rest on clinical performance and house officer training programs, the associations of clinical duty scheduling with insufficient rest, and the implications for risk management.Study designNarrative review.MethodsSeveral literature searches using broad terms such as “sleep deprivation,” “veterinary,” “physician,” and “surgeon” were performed using PubMed and Google scholar.ResultsSleep deprivation and insufficient rest have clear and deleterious effects on job performance, which in healthcare occupations impacts patient safety and practice function. The unique requirements of a career in veterinary surgery, which may include on‐call shifts and overnight work, can lead to distinct sleep challenges and chronic insufficient rest with resultant serious but often poorly recognized impacts. These effects negatively impact practices, teams, surgeons, and patients. The self‐assessment of fatigue and performance effect is demonstrably untrustworthy, reinforcing the need for institution‐level protections. While the issues are complex and there is no one‐size‐fits‐all approach, duty hour or workload restrictions may be an important first step in addressing these issues within veterinary surgery, as it has been in human medicine.ConclusionSystematic re‐examination of cultural expectations and practice logistics are needed if improvement in working hours, clinician well‐being, productivity, and patient safety are to occur.Clinical significance (or Impact)A more comprehensive understanding of the magnitude and consequence of sleep‐related impairment better enables surgeons and hospital management to address systemic challenges in veterinary practice and training programs.}, journal={VETERINARY SURGERY}, author={Steffey, Michele A. and Risselada, Marije and Scharf, Valery F. and Buote, Nicole J. and Zamprogno, Helia and Winter, Alexandra L. and Griffon, Dominique}, year={2023}, month={Feb} }
 @misc{steffey_griffon_risselada_buote_scharf_zamprogno_winter_2023, title={A narrative review of the physiology and health effects of burnout associated with veterinarian-pertinent occupational stressors}, volume={10}, ISSN={["2297-1769"]}, DOI={10.3389/fvets.2023.1184525}, abstractNote={Chronic workplace stress and burnout are serious problems in veterinary medicine. Although not classified as a medical condition, burnout can affect sleep patterns and contributes to chronic low grade systemic inflammation, autonomic imbalance, hormonal imbalances and immunodeficiencies, thereby increasing the risks of physical and psychological ill health in affected individuals. Cultural misconceptions in the profession often lead to perceptions of burnout as a personal failure, ideas that healthcare professionals are somehow at lower risk for suffering, and beliefs that affected individuals can or should somehow heal themselves. However, these concepts are antiquated, harmful and incorrect, preventing the design of appropriate solutions for this serious and growing challenge to the veterinary profession. Veterinarians must first correctly identify the nature of the problem and understand its causes and impacts before rational solutions can be implemented. In this first part of two companion reviews, burnout will be defined, pathophysiology discussed, and healthcare and veterinary-relevant occupational stressors that lead to burnout identified.}, journal={FRONTIERS IN VETERINARY SCIENCE}, author={Steffey, Michele A. and Griffon, Dominique J. and Risselada, Marije and Buote, Nicole J. and Scharf, Valery F. and Zamprogno, Helia and Winter, Alexandra L.}, year={2023}, month={Jul} }
 @article{hartman_hernandez_hendrix_risselada_weng_papich_kim_2023, title={Effects of storage up to 1 year on the in vitro antimicrobial activity of preformulated antibiotic-impregnated calcium sulfate beads}, volume={8}, ISSN={["1532-950X"]}, DOI={10.1111/vsu.14014}, abstractNote={AbstractObjectiveTo compare antimicrobial activity as demonstrated by the zone of inhibition (ZOI) produced by antibiotic‐impregnated calcium sulfate (CaSO4) beads after storage for 0, 3, 6, 9, and 12 months.Study designControlled laboratory study.Sample populationThree‐millimeter diameter CaSO4beads impregnated with vancomycin (125 mg/mL), or amikacin (250 mg/mL), or without antibiotic (control).MethodsCalcium sulfate beads were created at the onset of the study. Individual beads were separated in sterile containers and stored in a closed cabinet at room temperature and humidity for 0, 3, 6, 9, or 12 months until testing. The ZOI against methicillin‐resistantStaphylococcus pseudintermedius,methicillin‐resistantStaphylococcus aureus,andPseudomonas aeruginosawas recorded with serial replating on a fresh lawn of bacteria every 24 h until beads failed to produce a ZOI. The ZOIs and their changes were compared with mixed‐effects linear models. Eluted concentrations of vancomycin measured with high‐performance liquid chromatography were reported.ResultsAt 24 h, ZOIs were comparable regardless of time since formulation, except vancomycin againstP. aeruginosa,which failed to generate a ZOI. The daily changes of ZOI and duration of activity of antibiotics did not vary between storage length (p > .05). There was no consistent change in eluted drug concentration between storage length of beads.ConclusionLight protected storage at room temperature for up to 12 months did not impair the in vitro activity of antibiotic‐impregnated CaSO4beads, as demonstrated through ZOIs.Clinical significanceWhen stored correctly, antibiotic‐impregnated CaSO4beads can be used at least up to 12 months after formulation.}, journal={VETERINARY SURGERY}, author={Hartman, Emily Allyson and Hernandez, Daniela Pena and Hendrix, Gena Kenitra and Risselada, Marije and Weng, Hsin-Yi and Papich, Mark G. and Kim, Sun Young}, year={2023}, month={Aug} }
 @misc{steffey_griffon_risselada_scharf_buote_zamprogno_winter_2023, title={Veterinarian burnout demographics and organizational impacts: a narrative review}, volume={10}, ISSN={["2297-1769"]}, DOI={10.3389/fvets.2023.1184526}, abstractNote={Burnout is a work-related syndrome of physical and emotional exhaustion secondary to prolonged, unresolvable occupational stress. Individuals of different demographic cohorts may have disparate experiences of workplace stressors and burnout impacts. Healthcare organizations are adversely affected by burnt out workers through decreased productivity, low morale, suboptimal teamwork, and potential impacts on the quality of patient care. In this second of two companion reviews, the demographics of veterinary burnout and the impacts of burnout on affected individuals and work environments are summarized, before discussing mitigation concepts and their extrapolation for targeted strategies within the veterinary workplace and profession.}, journal={FRONTIERS IN VETERINARY SCIENCE}, author={Steffey, Michele A. and Griffon, Dominique J. and Risselada, Marije and Scharf, Valery F. and Buote, Nicole J. and Zamprogno, Helia and Winter, Alexandra L.}, year={2023}, month={Jul} }
 @article{putterman_selmic_kindra_duffy_risselada_phillips_2021, title={Influence of normograde versus retrograde catheterization of bile ducts in dogs treated for gallbladder mucocele}, volume={50}, ISSN={["1532-950X"]}, DOI={10.1111/vsu.13632}, abstractNote={AbstractObjectiveTo determine the influence of normograde (NG) versus retrograde (RG) catheterization of the cystic duct and common bile duct (CBD) in dogs with gallbladder mucoceles (GBM) treated with open cholecystectomy.Study designRetrospective study.AnimalsDogs (n = 117) with GBM.MethodsMedical records were reviewed for signalment, history, clinical laboratory and diagnostic imaging findings, details of surgery including catheterization method, complications, and outcome. Long‐term follow‐up data were obtained by telephone or electronic communication. Relationships between catheterization method and clinical variables and outcome were evaluated.ResultsDogs catheterized RG were more likely to experience any postoperative complication (p = .0004) including persistence of gastrointestinal signs (p = .0003). Survival to discharge and long‐term survival did not differ by group (p = .23 and p = .49). Total bilirubin (TB) decreased by 70.3% after NG catheterization compared to 39.1% after RG catheterization (p = .03) and increased in 14.9% dogs catheterized NG and 38.0% dogs catheterized RG (p = .004). The presence of a diplomate surgeon at surgery resulted in decreased incidences of any perioperative or postoperative complication (p = .003 and p = .05).ConclusionRetrograde catheterization was associated with more postoperative concerns than NG catheterization, but similar survival times. Surgery should be performed by diplomates experienced in biliary surgery to minimize complications.Clinical significanceAlthough both NG and RG techniques to catheterize the cystic duct and CBD are options for treatment of GBM with low mortality, results of this study provide some evidence to recommend NG over RG catheterization.}, number={4}, journal={VETERINARY SURGERY}, author={Putterman, Allison B. and Selmic, Laura E. and Kindra, Cameron and Duffy, Daniel J. and Risselada, Marije and Phillips, Heidi}, year={2021}, month={May}, pages={784–793} }
 @article{belda_ramos-vara_messenger_risselada_2021, title={Pharmacokinetic and safety assessment of carboplatin-impregnated calcium sulfate hemihydrate beads in eight rats}, ISSN={["1532-950X"]}, DOI={10.1111/vsu.13712}, abstractNote={AbstractObjectiveEvaluate local tissue toxicity and plasma platinum (Pt) in vivo after subcutaneous implantation of carboplatin‐impregnated calcium sulfate hemihydrate (CI‐CSH) beads.Study designIn vivo experimental study.AnimalsEight male Sprague–Dawley rats.MethodsCI‐CSH beads were implanted subcutaneously (5 mg carboplatin/rat; 13.5 mg/kg carboplatin; 7.08 mg/kg Pt; 1.18 mg/m2 Pt) in eight rats (d0). Wound healing (daily), radiographic bead dissolution (weekly), systemic Pt uptake (plasma‐Pt), local tissue Pt (d28), and histologic changes compared to nonincised and incised catheterization sites (d28) were assessed. Blood and tissue samples were analyzed by inductively coupled plasma mass spectrometry for Pt, and pharmacokinetic analysis was performed using noncompartmental methods.ResultsOne rat died at d10, the remainder survived until d28. No wound complications were seen. The CI‐CSH implantation site had higher histopathology scores than the other sites for necrosis (p = .013) and fibrosis (p = .013). Beads decreased in density radiographically (d0 to d28) (p = .062). Peak plasma‐Pt concentration was 225.78 ng/ml at 12 h, and decreased over time, but Pt was still detectable on d28. The elimination half‐life was 5.03 ± 1.13 days. Only 1.69% of implanted Pt remained in the beads at d28.ConclusionsCI‐CSH beads incited microscopic mild inflammation but wound healing was not impaired. Pt was absorbed systemically and the release from the beads was near complete at d28.Clinical significancePiled CI‐CSH bead implantation is well tolerated in rats with similar elution profile as previously described. Beads were radiographically visible at d28. Minimal Pt was detected systemically suggesting Pt release does not match bead dissolution.}, journal={VETERINARY SURGERY}, author={Belda, Beatriz and Ramos-Vara, Jose and Messenger, Kristen M. and Risselada, Marije}, year={2021}, month={Aug} }
 @article{risselada_tuohy_law_james_lascelles_2020, title={Local Administration of Carboplatin in Poloxamer 407 After an Ulnar Osteosarcoma Removal in a Dog}, volume={56}, ISSN={["1547-3317"]}, DOI={10.5326/JAAHA-MS-6926}, abstractNote={ABSTRACT
               An 8 yr old male castrated hound presented for a left distal ulnar osteosarcoma. Staging (computed tomography and nuclear scintigraphy) did not reveal any metastases. A limb-sparing ulnectomy with local adjunctive carboplatin in a poloxamer copolymer gel (poloxamer 407) was performed. The patient recovered without complications after surgery. No wound healing complications or adverse effects occurred after local use of carboplatin in poloxamer 407. The local recurrence-free interval was 296 days from surgery, and the survival time was 445 days from initial diagnosis. This is the first report in the veterinary literature of using poloxamer 407 as a carrier for local delivery of chemotherapeutic drugs in a clinical patient.}, number={6}, journal={JOURNAL OF THE AMERICAN ANIMAL HOSPITAL ASSOCIATION}, author={Risselada, Marije and Tuohy, Joanne L. and Law, Mac and James, Mindi L. and Lascelles, B. Duncan X.}, year={2020}, pages={325–330} }
 @article{dunlap_risselada_2019, title={Caudal Mediastinal Fish Hook Foreign Body with Pulmonary Artery Penetration in Two Dogs}, volume={55}, ISSN={["1547-3317"]}, DOI={10.5326/JAAHA-MS-6787}, abstractNote={ABSTRACT
               A 1.5 yr old spayed female mixed-breed dog and a 3 yr old spayed female Labrador retriever were evaluated for suspected esophageal fishhook foreign bodies. Radiographs yielded fishhook foreign bodies present caudal to the cardiac silhouette. Endoscopic retrieval for suspected caudal esophageal foreign body was attempted and aborted due to inadequate visualization of the entire fishhooks within the lumen of the esophagus. At surgery, the fishhooks were seen within the caudal mediastinum, and were engaging the left caudal pulmonary artery. Manual fishhook extraction was performed successfully with minimal hemorrhage. Fishhook foreign bodies caudal to the cardiac silhouette may have vascular involvement. Clinicians should exercise caution when attempting endoscopic retrieval of fishhooks in this location.}, number={1}, journal={JOURNAL OF THE AMERICAN ANIMAL HOSPITAL ASSOCIATION}, author={Dunlap, Anna E. and Risselada, Marije}, year={2019} }
 @article{mohiti-asli_risselada_jacob_pourdeyhimi_loboa_2017, title={Creation and Evaluation of New Porcine Model for Investigation of Treatments of Surgical Site Infection}, volume={23}, ISSN={["1937-3392"]}, DOI={10.1089/ten.tec.2017.0024}, abstractNote={Surgical site infection (SSI) is the most common cause of surgical failure, increasing the risks of postoperative mortality and morbidity. Recently, it has been reported that the use of antimicrobial dressings at the incision site help with prevention of SSI. Despite the increased body of research on the development of different types of antimicrobial dressings for this application, to our knowledge, nobody has reported a reliable large animal model to evaluate the efficacy of developed materials in a preclinical SSI model. In this study, we developed a porcine full-thickness incision model to investigate SSI caused by methicillin-resistant Staphylococcus aureus (MRSA), the leading cause of SSI in the United States. Using this model, we then evaluated the efficacy of our newly developed silver releasing nanofibrous dressings for preventing and inhibiting MRSA infection. Our results confirmed the ease and practicality of a new porcine model as an in vivo platform for evaluation of biomaterials for SSI. Using this model, we found that our silver releasing scaffolds significantly reduced bacterial growth in wounds inoculated with MRSA relative to nontreated controls and to wounds treated with the gold standard, silver sulfadiazine, without causing inflammation at the wound site. Findings from this study confirm the potential of our silver-releasing nanofibrous scaffolds for treatment/prevention of SSI, and introduce a new porcine model for in vivo evaluation of additional SSI treatment approaches.}, number={11}, journal={TISSUE ENGINEERING PART C-METHODS}, author={Mohiti-Asli, Mahsa and Risselada, Marije and Jacob, Megan and Pourdeyhimi, Behnam and Loboa, Elizabeth G.}, year={2017}, month={Nov}, pages={795–803} }
 @article{risselada_linder_griffith_roberts_davidson_zamboni_messenger_2017, title={Pharmacokinetics and toxicity of subcutaneous administration of carboplatin in poloxamer 407 in a rodent model pilot study}, volume={12}, number={10}, journal={PLoS One}, author={Risselada, M. and Linder, K. E. and Griffith, E. and Roberts, B. V. and Davidson, G. and Zamboni, W. C. and Messenger, K. M.}, year={2017} }
 @article{risselada_marcellin-little_messenger_griffith_davidson_papich_2016, title={Assessment of in vitro release of carboplatin from six carrier media}, volume={77}, ISSN={["1943-5681"]}, DOI={10.2460/ajvr.77.12.1381}, abstractNote={Abstract
OBJECTIVE To investigate in vitro carboplatin release from 6 carrier media.
SAMPLE 6 carboplatin-containing carrier media.
PROCEDURES An in vitro release study was performed with 6 commercially available carrier media: a hemostatic gelatin sponge, a poloxamer copolymer gel, and 2 sizes (3 and 4.8 mm in diameter) of beads molded from each of 2 commercial calcium sulfate products. All carrier media contained 10 mg of carboplatin. Carrier media specimens were placed in 37°C PBS solution for 96 hours. Carboplatin concentrations in PBS solution were measured by use of high-performance liquid chromatography at 15 time points to calculate the amount and proportion of carboplatin released from each specimen.
RESULTS Peak release of carboplatin from the poloxamer copolymer gel and hemostatic gelatin sponge were achieved after 4 and 20 hours, respectively. Maximum release did not differ significantly between the poloxamer copolymer gel and hemostatic gelatin sponge, but both released significantly more carboplatin within 96 hours than did both of the commercial calcium sulfate products. The poloxamer copolymer gel released 99% of the carboplatin, and the hemostatic gelatin sponge released 68.5% of the carboplatin. Peak release of carboplatin from the calcium sulfate beads was not reached within 96 hours.
CONCLUSIONS AND CLINICAL RELEVANCE In this study, carboplatin release from the hemostatic gelatin sponge was incomplete. The poloxamer copolymer gel and hemostatic gelatin sponge released carboplatin rapidly in vitro, whereas calcium sulfate beads did not.}, number={12}, journal={AMERICAN JOURNAL OF VETERINARY RESEARCH}, author={Risselada, Marije and Marcellin-Little, Denis J. and Messenger, Kristen M. and Griffith, Emily and Davidson, Gigi S. and Papich, Mark G.}, year={2016}, month={Dec}, pages={1381–1386} }
 @article{risselada_2016, title={Diagnosis and management of cholesteatomas in dogs}, volume={46}, number={4}, journal={Veterinary Clinics of North America. Small Animal Practice}, author={Risselada, M.}, year={2016}, pages={623-} }
 @article{risselada_mathews_griffith_2016, title={Effect of feline skin specimen preparation on postexcision and postfixation tissue shrinkage}, volume={18}, ISSN={["1532-2750"]}, DOI={10.1177/1098612x15602951}, abstractNote={Objectives This study was performed to assess skin–muscle–fascia specimen shrinkage and donor site changes, and to compare three techniques of specimen preparation for their effect on specimen shrinkage postexcision and after formalin fixation in feline cadaveric specimens. Methods Fifteen fresh feline cadavers were used for this study. Gelatin spheres were implanted in paired thoracic subcutaneous pockets and subsequently excised with 30 mm lateral margins and a fascial plane as the deep margin. Skin and fascia were either left unsutured, sutured together using four simple interrupted quadrant sutures (‘four-quadrant-sutured’) or sutured together in a continuous pattern (‘circumferentially sutured’). Specimens were measured for tumor-free margins on the excised and fixed specimens. The donor site defect was assessed for enlargement after specimen excision. Statistical analyses were performed to assess the donor site enlargement, and the influence of preparation technique on margin size, with significance set at P <0.05. Results The closest skin margins on the excised and fixed specimens were significantly smaller than the planned 30 mm margins; however, no significant difference was found between postexcision and postfixation specimens. No significant differences were found between the three techniques ( P = 0.74) with regard to margins either after excision or fixation. The fascial and skin defects of the donor site were significantly larger than the planned excision. Conclusions and relevance Surgically obtained feline skin–muscle–fascia specimens will significantly decrease in size prior to formalin fixation, resulting in falsely decreased measurements from tumor to tissue margins. Affixing the skin to the fascia does not significantly influence this decrease in margin size in feline tissue specimens at this location. }, number={12}, journal={JOURNAL OF FELINE MEDICINE AND SURGERY}, author={Risselada, Marije and Mathews, Kyle G. and Griffith, Emily}, year={2016}, month={Dec}, pages={970–975} }
 @article{risselada_mathews_griffith_2016, title={The effect of specimen preparation on post-excision and post-fixation dimensions, translation, and distortion of canine cadaver skin-muscle-fascia specimens}, volume={45}, DOI={10.1111/vsu.12481}, abstractNote={ObjectiveTo assess the effect of various tissue apposition techniques on specimens after formalin fixation, and to assess canine cadaveric composite skin‐muscle‐fascia specimen changes after excision without and with addition of a tumor model.Study DesignCanine cadaveric study; unbalanced paired design.Animals: Fresh canine cadavers (n = 30)MethodsExperiment 1: Paired 7 cm diameter, circular skin‐muscle‐fascia specimens were harvested bilaterally from three sites (thorax, abdomen, and thigh) on 10 cadavers. For each pair, the skin‐to‐fascia was sutured with a circumferential continuous pattern in one specimen and left unsutured in the other during excision. Specimen length and width were measured post‐excision and post‐fixation. Specimen rotation, translation, and distortion was measured post‐fixation. Experiment 2: A subcutaneous tumor model was implanted at 2 sites (neck, thorax) on 20 cadavers and specimens were then excised with 2 cm lateral margins and a deep fascial plane. Three suturing techniques of the skin‐to‐fascia at both sites were performed during excision: unsutured versus circumferential continuous suture (10 pairs) or circumferential versus 4 interrupted quadrant sutures (10 pairs). Lateral margins were compared post‐excision and post‐fixation.ResultsExperiment 1: There was significant reduction in the length and width of the fascia and skin post‐excision, but no further reduction after fixation. The circumferential suture decreased rotation and translation of the tissue layers but did not significantly affect specimen shrinkage or distortion compared with the unsutured specimen. Experiment 2: All 4 lateral margins decreased significantly post‐excision and post‐fixation compared with the planned pre‐excision margins. No difference was found between any of the 4 lateral margins for any skin‐to‐fascia suture technique.ConclusionCanine skin‐muscle‐fascia specimens significantly decrease in size from gross pre‐excision dimensions after removal. Suturing the specimen during surgery before excision will significantly improve alignment of tissue planes without creating distortion of the sample, but may alter the length, width, and lateral margins of the specimen.}, number={5}, journal={Veterinary Surgery}, author={Risselada, M. and Mathews, K. G. and Griffith, E.}, year={2016}, pages={563–570} }
 @article{risselada_ellison_winter_giglio_shih_hernandez_griffith_2015, title={In vitro evaluation of bursting pressure and intestinal luminal area of three jejunostomy tube placement techniques in dogs}, volume={76}, ISSN={["1943-5681"]}, DOI={10.2460/ajvr.76.5.467}, abstractNote={Abstract
OBJECTIVE To compare pursestring, Witzel (seromuscular inversion), and seromuscular incision jejunostomy tube placement techniques in vitro.
SAMPLE Jejunal specimens from 10 dogs.
PROCEDURES Jejunal segments (50 cm) were harvested immediately prior to euthanasia from 10 mixed-breed dogs Specimens were harvested with the orad and aborad ends clamped and stored in saline (0.9% NaCl) solution–soaked towels during instrumentation. Three jejunostomy tubes were placed via 3 techniques (pursestring, Witzel, and seromuscular incision), and 2 double lumen central venous catheters were placed at each intestinal end for luminal filling and leak testing. Intestinal luminal area was measured ultrasonographically with specimens suspended in a warm undyed saline solution bath with the intestinal lumen filled with dyed saline solution (intraluminal pressure, 6 mm Hg). Leak testing was performed by means of infusion of dyed saline solution (4 mL/min) until each jejunostomy site failed. Intestinal luminal area and leakage pressure were compared between the 3 tube placement techniques.
RESULTS The Witzel and seromuscular incision techniques decreased the intestinal luminal area measured at the tube insertion site, albeit nonsignificantly. For the seromuscular incision technique, a significant decrease in intestinal luminal area at the intraluminal site of measurement was found. For 2/30 specimens (1/10 pursestring and 1/10 seromuscular incision), failure occurred at pressures within the range of previously reported peak peristaltic pressure for dogs. Failure occurred at supraphysiologic peristaltic pressures for the remaining 28 specimens, including all 10 specimens for the Witzel technique.
CONCLUSIONS AND CLINICAL RELEVANCE In this in vitro study, all specimens for the Witzel technique withstood physiologic peristaltic pressures during leak testing. Both tunneling techniques (Witzel and seromuscular incision) created a decrease in intestinal luminal area. Further investigation, including in vivo testing, is indicated to evaluate the clinical relevance of these findings.}, number={5}, journal={AMERICAN JOURNAL OF VETERINARY RESEARCH}, author={Risselada, Marije and Ellison, Gary W. and Winter, Matthew D. and Giglio, Robson F. and Shih, Andre and Hernandez, Jorge A. and Griffith, Emily}, year={2015}, month={May}, pages={467–474} }
 @article{risselada_mathews_griffith_2015, title={Surgically planned versus histologically measured lateral tumor margins for resection of cutaneous and subcutaneous mast cell tumors in dogs: 46 cases (2010-2013)}, volume={247}, ISSN={["1943-569X"]}, DOI={10.2460/javma.247.2.184}, abstractNote={Abstract
Objective—To compare preplanned lateral surgical margins and measured lateral histologic margins for cutaneous and subcutaneous mast cell tumor (MCT) resections in dogs.
Design—Retrospective case series.
Sample—51 biopsy specimens from dogs (n = 46) with MCTs.
Procedures—All canine patients that underwent curative-intent surgical resection of cutaneous or subcutaneous MCTs from January 1, 2010, through June 30, 2013, with complete medical records including signalment, body condition score (BCS), surgery report (with measured surgical margins), and histopathology report were included. The surgically measured tumor margins in each quadrant were grouped and compared with the corresponding histologic margins. Specimens from dogs with truncal MCTs and a BCS of 7 to 9 on a scale from 1 to 9 (ie, high) were compared with those of dogs with a BCS of 4 to 6 to evaluate effect of BCS on tissue margins.
Results—51 specimens were included. Surgically mapped lateral margins differed significantly from histologically reported margins in all 4 quadrants. The mean histologic margins were 35% to 42% smaller than the surgical margins for the combined 51 specimens. A higher BCS did not significantly influence the magnitude of the decrease in lateral margins measured histologically. No significant difference was found for the magnitude of the differences between any of the 4 lateral margins.
Conclusions and Clinical Relevance—Results of this study suggested that surgical and histologic margins may differ significantly for canine cutaneous and subcutaneous MCTs. This may be a result of tissue shrinkage following excision and fixation, extension of the MCT beyond palpable margins, or both. Histologic measurements may significantly underestimate the tumor-free margins in dogs with cutaneous and subcutaneous MCTs. (J Am Vet Med Assoc 2015;247:184–189)}, number={2}, journal={JAVMA-JOURNAL OF THE AMERICAN VETERINARY MEDICAL ASSOCIATION}, author={Risselada, Marije and Mathews, Kyle G. and Griffith, Emily}, year={2015}, month={Jul}, pages={184–189} }