Works (34)

Updated: February 19th, 2026 21:46

2025 article

Guard Cell-Enriched Phosphoproteome Reveals Phosphorylation of Endomembrane Proteins in Closed Stomata

Pullen, A.-M., Lyons, S., Mordant, A., Herring, L., Akpa, B. S., & Rojas-Pierce, M. (2025, October 15).

topics (OpenAlex): Caveolin-1 and cellular processes; Parathyroid Disorders and Treatments
Source: ORCID
Added: November 1, 2025

2025 article

Manipulation of targeted protein degradation in plant biology

Rojas-Pierce, M., & Bednarek, S. Y. (2025, April 1). Biochemical Society Transactions, Vol. 4.

By: M. Rojas-Pierce n & S. Bednarek*

MeSH headings : Proteolysis; Plant Proteins / metabolism; Plants / metabolism; Ubiquitination; Ubiquitin-Protein Ligases / metabolism; Proteasome Endopeptidase Complex / metabolism
topics (OpenAlex): Protein Degradation and Inhibitors; Ubiquitin and proteasome pathways; Autophagy in Disease and Therapy
Sources: ORCID, Web Of Science, NC State University Libraries
Added: April 10, 2025

2025 article

Non-Thermal Plasma Activated Water is an Effective Nitrogen Fertilizer Alternative for Arabidopsis thaliana

Kizer, J., Robinson, C., Lucas, T. K., Shannon, S., Hernández, R., Stapelmann, K., & Rojas-Pierce, M. (2025, June 17). BioRxiv (Cold Spring Harbor Laboratory).

By: J. Kizer, C. Robinson, T. Lucas, S. Shannon*, R. Hernández, K. Stapelmann*, M. Rojas-Pierce*

topics (OpenAlex): Advanced Data Storage Technologies; Plasma Applications and Diagnostics
Source: ORCID
Added: June 17, 2025

2025 article

Non-thermal plasma activated water is an effective nitrogen fertilizer alternative for Arabidopsis thaliana

Kizer, J., Robinson, C., Lucas, T. K., Shannon, S., Hernández, R., Stapelmann, K., & Rojas-Pierce, M. (2025, September 8). (B. H. Samani, Ed.). PLoS ONE, Vol. 20.

By: J. Kizer n, C. Robinson n, T. Lucas n, S. Shannon n, R. Hernández n, K. Stapelmann n, M. Rojas-Pierce n

Ed(s): B. Samani

MeSH headings : Fertilizers; Arabidopsis / growth & development; Arabidopsis / metabolism; Arabidopsis / drug effects; Arabidopsis / genetics; Nitrogen / metabolism; Hydrogen Peroxide / pharmacology; Hydrogen Peroxide / metabolism; Water / chemistry; Water / pharmacology; Plasma Gases / chemistry; Plasma Gases / pharmacology; Reactive Oxygen Species / metabolism; Gene Expression Regulation, Plant / drug effects; Nitrates; Seedlings / growth & development; Seedlings / drug effects; Seedlings / metabolism; Plant Roots / metabolism; Plant Roots / drug effects; Plant Roots / growth & development
topics (OpenAlex): Plasma Applications and Diagnostics; Advanced Data Storage Technologies; Bluetooth and Wireless Communication Technologies
Sources: ORCID, Web Of Science, NC State University Libraries
Added: September 15, 2025

2025 article

Regulation of Vacuole Fusion in Stomata by Dephosphorylation of the HOPS subunit VPS39

Pullen, A.-M., Billings, G., Hodgens, C., White, G., Akpa, B. S., & Rojas-Pierce, M. (2025, October 2).

By: A. Pullen n, G. Billings n, C. Hodgens*, G. White n, B. Akpa* & M. Rojas-Pierce n

topics (OpenAlex): Bacteriophages and microbial interactions
Source: ORCID
Added: October 15, 2025

2025 article

Regulation of vacuole fusion in stomata by dephosphorylation of the HOPS subunit VPS39

Pullen, A. M., Billings, G., Hodgens, C., White, G., Akpa, B. S., & Rojas‐Pierce, M. (2025, December 1). The Plant Journal.

By: A. Pullen n, G. Billings n, C. Hodgens*, G. White n, B. Akpa* & M. Rojas‐Pierce n

MeSH headings : Vacuoles / metabolism; Plant Stomata / metabolism; Plant Stomata / physiology; Plant Stomata / genetics; Arabidopsis Proteins / metabolism; Arabidopsis Proteins / genetics; Arabidopsis / genetics; Arabidopsis / metabolism; Arabidopsis / physiology; Phosphorylation; Vesicular Transport Proteins / metabolism; Vesicular Transport Proteins / genetics; SNARE Proteins / metabolism; Membrane Fusion
topics (OpenAlex): Cellular transport and secretion; Plant nutrient uptake and metabolism; Plant Stress Responses and Tolerance
Sources: ORCID, NC State University Libraries
Added: December 21, 2025

2025 article

The Effect of Plasma Activated Water on the Rhizosphere Composition of Arabidopsis and Solanum lycopersicum

Kizer, J., Allen, X., Robinson, C., Grunden, A., Stapelmann, K., & Rojas-Pierce, M. (2025, October 15).

By: J. Kizer n, X. Allen n, C. Robinson n, A. Grunden n, K. Stapelmann n & M. Rojas-Pierce n

topics (OpenAlex): Magnetic and Electromagnetic Effects; Plant Genetic and Mutation Studies
Source: ORCID
Added: October 15, 2025

2024 article

Model-based inference of a dual role for HOPS in regulating guard cell vacuole fusion

Hodgens, C., Flaherty, D. T., Pullen, A.-M., Khan, I., English, N. J., Gillan, L., … Akpa, B. S. (2024, January 1). (X.-G. Zhu, Ed.). In Silico Plants, Vol. 6.

By: C. Hodgens*, D. Flaherty n, A. Pullen n, I. Khan n, N. English*, L. Gillan n, M. Rojas-Pierce n, B. Akpa n

Ed(s): X. Zhu

author keywords: Approximate Bayesian computation; parameter estimation; stomata; systems biology; vacuole fusion
topics (OpenAlex): Bacterial Genetics and Biotechnology; Vibrio bacteria research studies; CRISPR and Genetic Engineering
Sources: ORCID, Web Of Science, NC State University Libraries
Added: September 1, 2024

2024 article

Rapid depletion of target proteins in plants by an inducible protein degradation system

Huang, L., & Rojas-Pierce, M. (2024, March 6). The Plant Cell, Vol. 3.

By: L. Huang n & M. Rojas-Pierce n

MeSH headings : Nicotiana / metabolism; Nicotiana / genetics; Arabidopsis / metabolism; Arabidopsis / genetics; Proteolysis; Ubiquitin-Protein Ligases / metabolism; Ubiquitin-Protein Ligases / genetics; Arabidopsis Proteins / metabolism; Arabidopsis Proteins / genetics; Plants, Genetically Modified; Bacterial Proteins / metabolism; Bacterial Proteins / genetics; Gene Expression Regulation, Plant; Plant Proteins / metabolism; Plant Proteins / genetics; Protein Kinase C
topics (OpenAlex): Ubiquitin and proteasome pathways; Protein Degradation and Inhibitors; Plant Virus Research Studies
TL;DR: An inducible protein degradation system in plants named E3-DART for E3-targeted Degradation of Plant Proteins is described and may become an important tool in plant cell biology. (via Semantic Scholar)
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Sources: ORCID, Web Of Science, NC State University Libraries
Added: March 8, 2024

2024 article

White LED intensities during co-cultivation affect the Agrobacterium-mediated soybean (Glycine max) transformation using mature half seeds as explants

Shi, X., Li, B., Rojas-Pierce, M., & Hernández, R. (2024, November 26). (M. Pileggi, Ed.). PLoS ONE, Vol. 19.

By: X. Shi n, B. Li, M. Rojas-Pierce n & R. Hernández n

Ed(s): M. Pileggi

MeSH headings : Glycine max / genetics; Glycine max / growth & development; Transformation, Genetic; Seeds / genetics; Seeds / growth & development; Seeds / radiation effects; Light; Agrobacterium / genetics; Plants, Genetically Modified
topics (OpenAlex): Plant tissue culture and regeneration; Plant Genetic and Mutation Studies; Light effects on plants
Sources: Web Of Science, NC State University Libraries, ORCID
Added: November 26, 2024

2022 article

Microgravity enhances the phenotype of Arabidopsis zigzag-1 and reduces the Wortmannin-induced vacuole fusion in root cells

Wang, M., Danz, K., Ly, V., & Rojas-Pierce, M. (2022, September 5). Npj Microgravity, Vol. 9.

By: M. Wang n, K. Danz n, V. Ly n & M. Rojas-Pierce n

topics (OpenAlex): Polar Research and Ecology; Spaceflight effects on biology; Photosynthetic Processes and Mechanisms
TL;DR: Results indicate that microgravity enhances the zig-1 phenotype by reducing hypocotyl growth and vacuole fusion in some cells, and demonstrates the feasibility of chemical inhibitor treatments for plant cell biology experiments in space. (via Semantic Scholar)
Sources: ORCID, Web Of Science, NC State University Libraries
Added: September 6, 2022

2021 article

Molecular mechanisms of endomembrane trafficking in plants

Aniento, F., Medina Hernández, V. S., Dagdas, Y., Rojas-Pierce, M., & Russinova, E. (2021, September 17). The Plant Cell, Vol. 9.

MeSH headings : Autophagy; Biological Transport; Endocytosis; Endoplasmic Reticulum / metabolism; Golgi Apparatus / metabolism; Plant Physiological Phenomena; Vacuoles / metabolism
topics (OpenAlex): Plant nutrient uptake and metabolism; Polysaccharides and Plant Cell Walls; Legume Nitrogen Fixing Symbiosis
TL;DR: The current statet of knowledge on plant endomembrane trafficking is summarized, with a focus on four distinct trafficking pathways: ER-to-Golgi transport, endocytosis, trans-golgi network- to-vacuole transport, and autophagy. (via Semantic Scholar)
Sources: ORCID, Web Of Science, NC State University Libraries
Added: October 21, 2021

2020 article

Plasma agriculture: Review from the perspective of the plant and its ecosystem

Ranieri, P., Sponsel, N., Kizer, J., Rojas‐Pierce, M., Hernández, R., Gatiboni, L., … Stapelmann, K. (2020, September 30). Plasma Processes and Polymers, Vol. 18.

By: P. Ranieri n, N. Sponsel n, J. Kizer n, M. Rojas‐Pierce n, R. Hernández n, L. Gatiboni n, A. Grunden n, K. Stapelmann n

author keywords: plant fertilizer; nonthermal plasma; plant development; seed germination; soil microbiome
topics (OpenAlex): Plasma Applications and Diagnostics
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2. Zero Hunger (OpenAlex)
Source: ORCID
Added: October 1, 2020

2018 article

A whole-cell electron tomography model of vacuole biogenesis in Arabidopsis root cells

Cui, Y., Cao, W., He, Y., Zhao, Q., Wakazaki, M., Zhuang, X., … Jiang, L. (2018, December 11). Nature Plants, Vol. 5, pp. 95–105.

By: Y. Cui*, W. Cao*, Y. He*, Q. Zhao*, M. Wakazaki*, X. Zhuang*, J. Gao*, Y. Zeng* ...

Contributors: Y. Cui*, W. Cao*, Y. He*, Q. Zhao*, M. Wakazaki*, X. Zhuang*, J. Gao*, Y. Zeng* ...

MeSH headings : Arabidopsis / cytology; Arabidopsis Proteins / genetics; Arabidopsis Proteins / metabolism; Cell Fusion; Electron Microscope Tomography / methods; Guanine Nucleotide Exchange Factors / genetics; Imaging, Three-Dimensional; Multivesicular Bodies / metabolism; Mutation; Plant Cells; Plant Roots / cytology; Plants, Genetically Modified; SNARE Proteins / metabolism; Vacuoles / physiology; Vesicular Transport Proteins / genetics
topics (OpenAlex): Cellular transport and secretion; Photosynthetic Processes and Mechanisms; Calcium signaling and nucleotide metabolism
TL;DR: The vacuole in Arabidopsis root cells is presented at nanometre resolution by 3D whole-cell tomography, suggesting a cellular mechanism for plant vacuoles formation. (via Semantic Scholar)
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Source: ORCID
Added: January 30, 2019

2018 article

Phosphoinositides control the localization of HOPS subunit VPS41, which together with VPS33 mediates vacuole fusion in plants

Brillada, C., Zheng, J., Krüger, F., Rovira-Diaz, E., Askani, J. C., Schumacher, K., & Rojas-Pierce, M. (2018, August 13). Proceedings of the National Academy of Sciences, Vol. 115, pp. E8305–E8314.

By: C. Brillada n, J. Zheng n, F. Krüger*, E. Rovira-Diaz n, J. Askani*, K. Schumacher*, M. Rojas-Pierce n

Contributors: C. Brillada n, J. Zheng n, F. Krüger*, E. Rovira-Diaz n, J. Askani*, K. Schumacher*, M. Rojas-Pierce n

author keywords: Arabidopsis; vacuole fusion; HOPS; SNARE; phosphoinositides
MeSH headings : Arabidopsis / genetics; Arabidopsis / metabolism; Arabidopsis Proteins / genetics; Arabidopsis Proteins / metabolism; Membrane Fusion / physiology; Membrane Proteins / genetics; Membrane Proteins / metabolism; Phosphatidylinositol Phosphates / genetics; Phosphatidylinositol Phosphates / metabolism; Vacuoles / genetics; Vacuoles / metabolism; Vesicular Transport Proteins / genetics; Vesicular Transport Proteins / metabolism
topics (OpenAlex): Plant Molecular Biology Research; Legume Nitrogen Fixing Symbiosis; Photosynthetic Processes and Mechanisms
TL;DR: It is shown that Arabidopsis HOPS subunits VPS33 and VPS41 accumulate in late endosomes and that VPS 41, but not VPS 33, accumulates in the tonoplast via a wortmannin-sensitive process, and that HOPS–SNARE interactions at the initial stage are similar to other eukaryotes. (via Semantic Scholar)
Sources: Web Of Science, ORCID, NC State University Libraries
Added: October 19, 2018

2017 article

Vacuolar trafficking and biogenesis: a maturation in the field

Brillada, C., & Rojas-Pierce, M. (2017, September 1). Current Opinion in Plant Biology, Vol. 40, pp. 77–81.

By: C. Brillada n & M. Rojas-Pierce n

Contributors: C. Brillada n & M. Rojas-Pierce n

MeSH headings : Organelle Biogenesis; Plant Physiological Phenomena; Plant Proteins / metabolism; Protein Transport; Vacuoles / metabolism
topics (OpenAlex): Cellular transport and secretion; Photosynthetic Processes and Mechanisms; Protist diversity and phylogeny
TL;DR: Recent evidence is summarized that indicates conserved and plant-specific mechanisms involved in sorting and trafficking of proteins to this major organelle are indicated. (via Semantic Scholar)
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Sources: Web Of Science, ORCID, NC State University Libraries
Added: August 6, 2018

2016 article

Modifications to a LATE MERISTEM IDENTITY-1 gene are responsible for the major leaf shapes of cotton

Andres, R., Coneva, V., Frank, M. H., Tuttle, J. R., Han, S.-W., Samayoa, L. F., … Kuraparthy, V. (2016, July 7). Proceedings of the National Academy of Sciences of the United States of America, Vol. 114, pp. E57–66.

By: R. Andres n, V. Coneva*, M. Frank*, J. Tuttle n, S. Han n, L. Samayoa n, B. Kaur n, L. Zhu n ...

topics (OpenAlex): Research in Cotton Cultivation; Plant Virus Research Studies; Plant Molecular Biology Research; Plant Gene Expression Analysis
TL;DR: It is shown that the major leaf shapes of cotton at the L-D1 locus are controlled by a HD-Zip transcription factor most similar to Late Meristem Identity1 (LMI1) gene, and that sub-okra is the ancestral leaf shape of tetraploid cotton and normal is a derived mutant allele that came to predominate and define the leafshape of cultivated cotton. (via Semantic Scholar)
Sources: NC State University Libraries, NC State University Libraries
Added: August 6, 2018

2016 journal article

Modifications to a LATE MERISTEM IDENTITY1 gene are responsible for the major leaf shapes of Upland cotton (Gossypium hirsutum L.)

Proceedings of the National Academy of Sciences, 114(1), E57–E66.

By: R. Andres n, V. Coneva*, M. Frank*, J. Tuttle n, L. Samayoa n, S. Han n, B. Kaur n, L. Zhu n ...

Contributors: R. Andres n, V. Coneva*, M. Frank*, J. Tuttle n, L. Samayoa n, S. Han n, B. Kaur n, L. Zhu n ...

author keywords: cotton; leaf shape; okra; gene cloning
MeSH headings : Amino Acid Sequence / genetics; Frameshift Mutation / genetics; Gene Expression Regulation, Plant; Genes, Plant / genetics; Gossypium / genetics; Gossypium / physiology; Plant Leaves / genetics; Plant Leaves / physiology; Promoter Regions, Genetic / genetics; Transcription Factors / genetics
topics (OpenAlex): Research in Cotton Cultivation; Plant Molecular Biology Research; Plant Virus Research Studies
TL;DR: The results indicate that subokra is the ancestral leaf shape of tetraploid cotton that gave rise to the okra allele and that normal is a derived mutant allele that came to predominate and define the leafshape of cultivated cotton. (via Semantic Scholar)
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UN Sustainable Development Goal Categories
2. Zero Hunger (Web of Science)
13. Climate Action (Web of Science; OpenAlex)
Sources: ORCID, Crossref, NC State University Libraries
Added: January 30, 2019

2016 article

Wortmannin-induced vacuole fusion enhances amyloplast dynamics in Arabidopsiszigzag1hypocotyls

Alvarez, A. A., Han, S. W., Toyota, M., Brillada, C., Zheng, J., Gilroy, S., & Rojas-Pierce, M. (2016, October 20). Journal of Experimental Botany, Vol. 67, pp. 6459–6472.

By: A. Alvarez n, S. Han n, M. Toyota*, C. Brillada n, J. Zheng n, S. Gilroy*, M. Rojas-Pierce n

Contributors: A. Alvarez n, S. Han n, M. Toyota*, C. Brillada n, J. Zheng n, S. Gilroy*, M. Rojas-Pierce n

author keywords: Amyloplasts; cytoskeleton; gravitropism; SNARE; vacuole; wortmannin
MeSH headings : Androstadienes / pharmacology; Arabidopsis / drug effects; Arabidopsis / growth & development; Arabidopsis / ultrastructure; Arabidopsis Proteins / genetics; Arabidopsis Proteins / physiology; Gravitropism / drug effects; Gravitropism / physiology; Hypocotyl / drug effects; Hypocotyl / growth & development; Microscopy; Qb-SNARE Proteins / genetics; Qb-SNARE Proteins / physiology; Vacuoles / drug effects; Vacuoles / physiology; Vacuoles / ultrastructure; Wortmannin
topics (OpenAlex): Plant Molecular Biology Research; Photosynthetic Processes and Mechanisms; Light effects on plants
TL;DR: The highly restricted movement of amyloplasts in graviperceptive cells of zig-1 hypocotyls is overcome by wortmannin treatment, suggesting a physical association between amylplasts and the vacuole. (via Semantic Scholar)
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UN Sustainable Development Goal Categories
2. Zero Hunger (Web of Science)
13. Climate Action (Web of Science)
Sources: Web Of Science, ORCID, NC State University Libraries
Added: August 6, 2018

2015 article

REGULATOR OF BULB BIOGENESIS1 (RBB1) Is Involved in Vacuole Bulb Formation in Arabidopsis

Han, S. W., Alonso, J. M., & Rojas-Pierce, M. (2015, April 27). (D. Bassham, Ed.). PLoS ONE, Vol. 10, p. e0125621.

By: S. Han n, J. Alonso n & M. Rojas-Pierce n

Contributors: S. Han n, J. Alonso n & M. Rojas-Pierce n

Ed(s): D. Bassham

MeSH headings : Arabidopsis / physiology; Arabidopsis / ultrastructure; Arabidopsis Proteins / physiology; Blotting, Western; Cloning, Molecular; Cytosol / physiology; Microscopy, Confocal; Polymerase Chain Reaction; Vacuoles / physiology; Vacuoles / ultrastructure
topics (OpenAlex): Photosynthetic Processes and Mechanisms; Plant nutrient uptake and metabolism; Plant Molecular Biology Research
TL;DR: It is proposed that the bulbs may function transiently to accommodate membranes and proteins when transvacuolar strands fail to elongate, and it is shown that RBB1 corresponds to a very large protein of unknown function that is specific to plants, is present in the cytosol, and may associate with cellular membranes. (via Semantic Scholar)
Sources: NC State University Libraries, ORCID, NC State University Libraries
Added: August 6, 2018

2014 article

Homotypic Vacuole Fusion Requires VTI11 and Is Regulated by Phosphoinositides

Zheng, J., Han, S. W., Rodriguez-Welsh, M. F., & Rojas-Pierce, M. (2014, February 25). Molecular Plant, Vol. 7, pp. 1026–1040.

By: J. Zheng n, S. Han n, M. Rodriguez-Welsh n & M. Rojas-Pierce*

Contributors: J. Zheng n, S. Han n, M. Rodriguez-Welsh n & M. Rojas-Pierce*

author keywords: vacuole; SNARE; phosphoinositides; membrane fusion; Wortmannin
MeSH headings : Androstadienes / pharmacology; Arabidopsis / drug effects; Arabidopsis / metabolism; Arabidopsis Proteins / metabolism; Chromones / pharmacology; Morpholines / pharmacology; Phosphatidylinositol Phosphates / metabolism; Phosphoinositide-3 Kinase Inhibitors; Qb-SNARE Proteins / metabolism; Vacuoles / metabolism; Wortmannin
topics (OpenAlex): Cellular transport and secretion; Legume Nitrogen Fixing Symbiosis; Studies on Chitinases and Chitosanases
TL;DR: A ubiquitous role of phosphoinositides in vacuole fusion is suggested, both during the development of the large central vacuoles and during the dynamic vacUole remodeling that occurs as part of stomata movements. (via Semantic Scholar)
Sources: Web Of Science, ORCID, NC State University Libraries
Added: August 6, 2018

2014 article

Multiple vacuoles inimpaired tonoplast trafficking3mutants are independent organelles

Zheng, J., Han, S. W., Munnik, T., & Rojas-Pierce, M. (2014, August 13). Plant Signaling & Behavior, Vol. 9, p. e972113.

By: J. Zheng n, S. Han n, T. Munnik* & M. Rojas-Pierce n

Contributors: J. Zheng n, S. Han n, T. Munnik* & M. Rojas-Pierce n

author keywords: vacuoles; phosphoinositides; SNARE; Wortmannin; membrane fusion
MeSH headings : Androstadienes / pharmacology; Arabidopsis / drug effects; Arabidopsis / metabolism; Arabidopsis Proteins / genetics; Arabidopsis Proteins / metabolism; Mutation / genetics; Plant Epidermis / cytology; Plant Epidermis / drug effects; Plant Roots / drug effects; Plant Roots / metabolism; Recombinant Fusion Proteins / metabolism; Time-Lapse Imaging; Vacuoles / metabolism; Wortmannin
topics (OpenAlex): Cellular transport and secretion; Photosynthetic Processes and Mechanisms; Calcium signaling and nucleotide metabolism
TL;DR: Fluorescence Recovery After Photobleaching (FRAP) is used to demonstrate that the vacuoles in itt3/vti11 are independent organelles and provides supporting evidence for a critical role of PtdIns(3)P in vacuole fusion in roots and guard cells. (via Semantic Scholar)
Source: ORCID
Added: January 29, 2019

2014 article

PLASTID MOVEMENT IMPAIRED1 mediates ABA sensitivity during germination and implicates ABA in light-mediated Chloroplast movements

Rojas-Pierce, M., Whippo, C. W., Davis, P. A., Hangarter, R. P., & Springer, P. S. (2014, August 1). Plant Physiology and Biochemistry, Vol. 83, pp. 185–193.

Contributors: M. Rojas-Pierce n, C. Whippo*, P. Davis*, R. Hangarter* & P. Springer*

author keywords: ABA; Arabidopsis; Chloroplast movement; Seed germination; Water deficit
MeSH headings : Abscisic Acid / genetics; Abscisic Acid / metabolism; Arabidopsis / genetics; Arabidopsis / metabolism; Arabidopsis Proteins / genetics; Arabidopsis Proteins / metabolism; Chloroplasts / genetics; Chloroplasts / metabolism; Intracellular Signaling Peptides and Proteins / genetics; Intracellular Signaling Peptides and Proteins / metabolism; Light; Mutation; Signal Transduction / genetics; Signal Transduction / radiation effects
topics (OpenAlex): Light effects on plants; Plant Molecular Biology Research; Plant Stress Responses and Tolerance
TL;DR: Evidence is provided that PMI1 is involved in the regulation of seed germination by ABA, acting upstream of the intersection between ABA and low-glucose signaling pathways, and the combined phenotypes of pmi1 mutants in chloroplast movement and ABA responses indicate that ABA signaling may modulatechloroplast motility. (via Semantic Scholar)
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UN Sustainable Development Goal Categories
2. Zero Hunger (Web of Science)
6. Clean Water and Sanitation (OpenAlex)
13. Climate Action (Web of Science)
Sources: Web Of Science, ORCID, NC State University Libraries
Added: August 6, 2018

2013 article

Targeting of tonoplast proteins to the vacuole

Rojas-Pierce, M. (2013, July 18). Plant Science, Vol. 211, pp. 132–136.

By: M. Rojas-Pierce n

Contributors: M. Rojas-Pierce n

author keywords: Vacuole; Protein trafficking; Tonoplast protein
MeSH headings : Cell Membrane / metabolism; Endoplasmic Reticulum / metabolism; Golgi Apparatus / metabolism; Intracellular Membranes / metabolism; Membrane Proteins / metabolism; Plant Proteins / metabolism; Plants / metabolism; Protein Transport; Vacuoles / metabolism
topics (OpenAlex): Cellular transport and secretion; Photosynthetic Processes and Mechanisms; Calcium signaling and nucleotide metabolism
TL;DR: A clearer picture for targeting mechanisms for tonoplast proteins is starting to emerge, and recent reports indicate that the Golgi-dependent and independent pathways may merge at the level of the pre-vacuolar compartment. (via Semantic Scholar)
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Sources: Web Of Science, ORCID, NC State University Libraries
Added: August 6, 2018

2012 article

A Small Molecule Inhibitor Partitions Two Distinct Pathways for Trafficking of Tonoplast Intrinsic Proteins in Arabidopsis

Rivera-Serrano, E. E., Rodriguez-Welsh, M. F., Hicks, G. R., & Rojas-Pierce, M. (2012, September 5). (E. J. M. Damme, Ed.). PLoS ONE, Vol. 7, p. e44735.

By: E. Rivera-Serrano n, M. Rodriguez-Welsh n, G. Hicks* & M. Rojas-Pierce n

Contributors: E. Rivera-Serrano n, M. Rodriguez-Welsh n, G. Hicks* & M. Rojas-Pierce n

Ed(s): E. Damme n

MeSH headings : Acyltransferases / genetics; Acyltransferases / physiology; Aquaporins / genetics; Aquaporins / physiology; Arabidopsis / drug effects; Arabidopsis / metabolism; Arabidopsis Proteins / genetics; Arabidopsis Proteins / metabolism; Arabidopsis Proteins / physiology; Brefeldin A / pharmacology; Cell Membrane / metabolism; Golgi Apparatus / metabolism; Green Fluorescent Proteins / metabolism; Hypocotyl / metabolism; Microscopy, Confocal / methods; Models, Biological; Promoter Regions, Genetic; Protein Transport; Seedlings; Vacuoles / metabolism
topics (OpenAlex): Plant Molecular Biology Research; Photosynthetic Processes and Mechanisms; Plant nutrient uptake and metabolism
TL;DR: It is shown that members of the TIP family are targeted to the vacuole via at least two distinct pathways, and the bioactivity of a novel inhibitor that can differentiate between them is characterized and mediated in part by common mechanisms. (via Semantic Scholar)
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6. Clean Water and Sanitation (OpenAlex)
Sources: Web Of Science, ORCID, NC State University Libraries
Added: August 6, 2018

2012 article

Increasing phosphatidylinositol (4,5) bisphosphate biosynthesis affects plant nuclear lipids and nuclear functions

Dieck, C. B., Wood, A., Brglez, I., Rojas-Pierce, M., & Boss, W. F. (2012, May 17). Plant Physiology and Biochemistry, Vol. 57, pp. 32–44.

By: C. Dieck n, A. Wood n, I. Brglez n, M. Rojas-Pierce n & W. Boss n

Contributors: C. Dieck n, A. Wood n, I. Brglez n, M. Rojas-Pierce n & W. Boss n

author keywords: Plant nuclei; Phospholipids; Histone; Phosphatidylinositol (4,5) bisphosphate; Plasma membrane
MeSH headings : Cell Membrane / metabolism; Cell Nucleus / metabolism; Phosphatidylcholines / metabolism; Phosphatidylinositol 4,5-Diphosphate / metabolism; Phosphatidylserines / metabolism; Phospholipids / metabolism; Plants / metabolism; Tobacco / metabolism
topics (OpenAlex): Lipid metabolism and biosynthesis; Cellular transport and secretion; Nuclear Structure and Function
TL;DR: The lipid profiles revealed similarities and differences in the plasma membrane and nuclei from the NT and transgenic HK cell lines and a notable characteristic of nuclear lipids from both cell types is that PtdIns accounts for a higher mol% of total lipids compared to that of the protoplast PM lipids. (via Semantic Scholar)
Sources: Web Of Science, ORCID, NC State University Libraries
Added: August 6, 2018

2008 journal article

Arabidopsis P-Glycoprotein19 Participates in the Inhibition of Gravitropism by Gravacin

Chemistry & Biology, 15(1), 87.

By: M. Rojas-Pierce*, B. Titapiwatanakun, E. Sohn, F. Fang, C. Larive, J. Blakeslee, Y. Cheng, S. Cuttler ...

topics (OpenAlex): Plant Reproductive Biology; Photosynthetic Processes and Mechanisms; Plant Stress Responses and Tolerance
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UN Sustainable Development Goal Categories
2. Zero Hunger (OpenAlex)
Sources: Crossref, NC State University Libraries
Added: March 5, 2020

2008 article

Preparation of Methyl Ester Precursors of Biologically Active Agents

Zou, Y., Rojas-Pierce, M., Raikhel, N. V., & Pirrung, M. C. (2008, March 1). BioTechniques, Vol. 44, pp. 377–384.

By: Y. Zou*, M. Rojas-Pierce*, N. Raikhel* & M. Pirrung*

Contributors: Y. Zou*, M. Rojas-Pierce*, N. Raikhel* & M. Pirrung*

MeSH headings : Carboxylic Acids / chemistry; Esters / chemistry; Ion Exchange Resins / chemistry; Methylation; Prodrugs / chemistry
topics (OpenAlex): Chemical Synthesis and Analysis; Analytical Chemistry and Chromatography; Innovative Microfluidic and Catalytic Techniques Innovation
TL;DR: This method enables scientists to easily convert biologically active carboxylic acids into their methyl esters ("pro-drugs" generally having improved ability to penetrate cell membranes) using only equipment commonly found in a biology laboratory. (via Semantic Scholar)
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6. Clean Water and Sanitation (OpenAlex)
Source: ORCID
Added: January 29, 2019

2007 article

Arabidopsis P-Glycoprotein19 Participates in the Inhibition of Gravitropism by Gravacin

Rojas-Pierce, M., Titapiwatanakun, B., Sohn, E. J., Fang, F., Larive, C. K., Blakeslee, J., … Raikhel, N. V. (2007, December 1). Chemistry & Biology, Vol. 14, pp. 1366–1376.

By: M. Rojas-Pierce*, B. Titapiwatanakun*, E. Sohn*, F. Fang*, C. Larive*, J. Blakeslee*, Y. Cheng*, S. Cuttler* ...

Contributors: M. Rojas-Pierce*, B. Titapiwatanakun*, E. Sohn*, F. Fang*, C. Larive*, J. Blakeslee*, Y. Cheng*, S. Cuttler* ...

MeSH headings : ATP-Binding Cassette Transporters / antagonists & inhibitors; ATP-Binding Cassette Transporters / genetics; ATP-Binding Cassette Transporters / metabolism; Aquaporins / metabolism; Arabidopsis / drug effects; Arabidopsis / genetics; Arabidopsis / metabolism; Arabidopsis Proteins / antagonists & inhibitors; Arabidopsis Proteins / genetics; Arabidopsis Proteins / metabolism; Binding, Competitive / physiology; Biological Transport / drug effects; Cell Membrane / metabolism; Furans / metabolism; Furans / pharmacology; Gravitropism / drug effects; HeLa Cells; Humans; Hypocotyl / drug effects; Hypocotyl / genetics; Hypocotyl / metabolism; Indoleacetic Acids / metabolism; Membrane Transport Proteins / genetics; Membrane Transport Proteins / metabolism; Microsomes / metabolism; Molecular Structure; Mutation; Phthalimides / metabolism; Plant Shoots / drug effects; Plant Shoots / genetics; Plant Shoots / metabolism; Protein Binding / drug effects; Protein Transport / drug effects; Recombinant Proteins / metabolism; Transfection
topics (OpenAlex): Plant Molecular Biology Research; Plant Reproductive Biology; Lipid metabolism and biosynthesis
TL;DR: It is demonstrated that P-glycoprotein19 (PGP19) is a target for Gravacin and participates in its inhibition of gravitropism and E1174 as an important residue for PGP19 activity and its ability to form active transport complexes with PIN1. (via Semantic Scholar)
Source: ORCID
Added: January 29, 2019

2007 article

The shoot meristem identity gene TFL1 is involved in flower development and trafficking to the protein storage vacuole

Sohn, E. J., Rojas-Pierce, M., Pan, S., Carter, C., Serrano-Mislata, A., Madueño, F., … Raikhel, N. V. (2007, November 15). Proceedings of the National Academy of Sciences, Vol. 104, pp. 18801–18806.

By: E. Sohn*, M. Rojas-Pierce*, S. Pan*, C. Carter*, A. Serrano-Mislata*, F. Madueño*, E. Rojo*, M. Surpin*, N. Raikhel*

Contributors: J. Eun*, M. Rojas-Pierce*, S. Pan*, C. Carter*, A. Serrano-Mislata*, F. Madueño*, E. Rojo*, M. Surpin*, N. Raikhel*

author keywords: protein trafficking; vegetative tissue; adaptin
MeSH headings : Arabidopsis / genetics; Arabidopsis / growth & development; Arabidopsis / metabolism; Arabidopsis / ultrastructure; Arabidopsis Proteins / genetics; Arabidopsis Proteins / metabolism; Flowers / genetics; Flowers / growth & development; Flowers / metabolism; Gene Expression Regulation, Plant; Meristem / genetics; Meristem / metabolism; Microscopy, Electron, Transmission; Microscopy, Immunoelectron; Protein Transport; Vacuoles / genetics; Vacuoles / metabolism; Vacuoles / ultrastructure
topics (OpenAlex): Plant Molecular Biology Research; Plant Reproductive Biology; Cellular transport and secretion
TL;DR: The results suggest a developmental role for the PSV in vegetative tissues and show that an Arabidopsis thaliana mutant that disrupts PSV trafficking identified TERMINAL FLOWER 1 (TFL1), a shoot meristem identity gene. (via Semantic Scholar)
Source: ORCID
Added: January 30, 2019

2006 article

Quantitative Trait Loci for Root Architecture Traits Correlated with Phosphorus Acquisition in Common Bean

Beebe, S. E., Rojas‐Pierce, M., Yan, X., Blair, M. W., Pedraza, F., Muñoz, F., … Lynch, J. P. (2006, January 1). Crop Science, Vol. 46, p. 413.

topics (OpenAlex): Plant nutrient uptake and metabolism; Legume Nitrogen Fixing Symbiosis; Plant pathogens and resistance mechanisms
TL;DR: The results confirm the importance of root structure for LP adaptation and highlight the need for a more detailed understanding of root architectural traits for phenotypic as well as marker-aided selection of more P-efficient crops. (via Semantic Scholar)
UN Sustainable Development Goals Color Wheel
UN Sustainable Development Goal Categories
15. Life on Land (OpenAlex)
Source: ORCID
Added: January 29, 2019

2005 article

The power of chemical genomics to study the link between endomembrane system components and the gravitropic response

Surpin, M., Rojas-Pierce, M., Carter, C., Hicks, G. R., Vasquez, J., & Raikhel, N. V. (2005, March 16). Proceedings of the National Academy of Sciences, Vol. 102, pp. 4902–4907.

By: M. Surpin*, M. Rojas-Pierce*, C. Carter*, G. Hicks*, J. Vasquez* & N. Raikhel*

Contributors: M. Surpin*, M. Rojas-Pierce*, C. Carter*, G. Hicks*, J. Vasquez* & N. Raikhel*

author keywords: chemicals; GFP; gravitropism; trafficking; vacuoles
MeSH headings : Arabidopsis / drug effects; Arabidopsis / genetics; Arabidopsis / growth & development; Biological Transport / drug effects; Biological Transport / genetics; Biological Transport / physiology; Genomic Library; Genomics / methods; Gravitropism / drug effects; Gravitropism / genetics; Gravitropism / physiology; Indoleacetic Acids / pharmacology; Intracellular Membranes / physiology; Intracellular Membranes / ultrastructure; Microscopy, Confocal; Plant Proteins / isolation & purification; Plant Proteins / pharmacology
topics (OpenAlex): Pancreatic function and diabetes; RNA and protein synthesis mechanisms; RNA Research and Splicing
TL;DR: A library of 10,000 diverse chemicals for compounds that affected the gravitropism of Arabidopsis seedlings positively or negatively and four of the 34 compounds were found to cause aberrant endomembrane morphologies. (via Semantic Scholar)
Source: ORCID
Added: January 30, 2019

2003 article

Gene and Enhancer Traps for Gene Discovery

Rojas-Pierce, M., & Springer, P. S. (2003, November 15). Humana Press EBooks, pp. 221–240.

By: M. Rojas-Pierce* & P. Springer*

MeSH headings : Base Sequence; DNA Primers; DNA Transposable Elements; DNA, Plant / chemistry; DNA, Plant / genetics; Enhancer Elements, Genetic / genetics; Gene Expression Profiling / methods; Genes, Plant / genetics; Genes, Reporter; Genetic Techniques; Glucuronidase / genetics; Indicators and Reagents; Plants, Genetically Modified / genetics; Polymerase Chain Reaction / methods
topics (OpenAlex): CRISPR and Genetic Engineering; Animal Genetics and Reproduction; Genomics and Chromatin Dynamics
TL;DR: In this chapter, protocols for examining and documenting GUS reporter gene activity in individual lines are described and methods for the amplification of sequences flanking transposant insertions and subsequent molecular and genetic characterization of individual insertions are provided. (via Semantic Scholar)
Source: ORCID
Added: January 29, 2019

2003 journal article

Gene and enhancer traps for gene discovery.

Methods in Molecular Biology (Clifton, N.J.), 236, 221–240. http://www.scopus.com/inward/record.url?eid=2-s2.0-1542548180&partnerID=MN8TOARS

By: M. Rojas-Pierce & P. Springer

Contributors: M. Rojas-Pierce & P. Springer

Source: ORCID
Added: January 30, 2019

Employment

Updated: February 5th, 2025 11:56

2020 - present

North Carolina State University Raleigh, US
Professor Plant and Microbial Biology

2015 - 2020

NC State University Raleigh, NC, US
Associate Professor Department of Plant and Microbial Biology

2008 - 2015

North Carolina State University Raleigh, NC, US
Assistant Professor Department of Plant and Microbial Biology

2003 - 2008

University of California Riverside, CA, US
Postdoctoral Scholar Department of Botany and Plant Science

Education

Updated: December 19th, 2019 12:18

University of California Riverside, CA, US
Ph.D. Plant Biology Botany and Plant Science

Universidad de los Andes Bogota, CO
B.Sc. Biology Departamento de Ciencias Biologicas

Funding History

Funding history based on the linked ORCID record. Updated: December 19th, 2019 12:12

grant August 1, 2019 - July 31, 2022
Vacuole remodeling in guard cells during stomata movements
Directorate for Biological Sciences
grant April 1, 2019 - March 31, 2021
EAGER: An inducible degron system for control of protein degradation in plants.
Directorate for Biological Sciences
grant October 29, 2018 - October 28, 2021
Membrane Contacts in Plant Gravity Perception
National Aeronautics and Space Administration
grant September 1, 2016 - August 31, 2020
MRI:Acquisition of a Zeiss LSM 880 confocal microscope with Airyscan for research and training at North Carolina State University.
Directorate for Biological Sciences
grant July 2, 2013 - July 1, 2018
The Role of Vacuole Membrane Fusion in Plant Gravity Perception
National Aeronautics and Space Administration
grant February 1, 2013 - January 31, 2017
Tonoplast Protein Trafficking in Arabidopsis
Directorate for Biological Sciences
grant February 15, 2010 - January 31, 2012
Tonoplast Protein Trafficking in Arabidopsis
Directorate for Biological Sciences
grant August 1, 2008 - January 31, 2011
Identifying Targets/Pathways of Chemical Probes for the Plant Endomembrane System
Directorate for Biological Sciences

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