@article{pierce_graham_stiff_osborne_haigler_2019, title={Cultures of Gossypium barbadense cotton ovules offer insights into the microtubule-mediated control of fiber cell expansion}, volume={249}, ISSN={0032-0935 1432-2048}, url={http://dx.doi.org/10.1007/s00425-019-03106-5}, DOI={10.1007/s00425-019-03106-5}, abstractNote={A novel method for culturing ovules of Gossypium barbadense allowed in vitro comparisons with Gossypium hirsutum and revealed variable roles of microtubules in controlling cotton fiber cell expansion. Cotton fibers undergo extensive elongation and secondary wall thickening as they develop into our most important renewable textile material. These single cells elongate at the apex as well as elongating and expanding in diameter behind the apex. These multiple growth modes represent an interesting difference compared to classical tip-growing cells that needs to be explored further. In vitro ovule culture enables experimental analysis of the controls of cotton fiber development in commonly grown Gossypium hirsutum cotton, but, previously, there was no equivalent system for G. barbadense, which produces higher quality cotton fiber. Here, we describe: (a) how to culture the ovules of G. barbadense successfully, and (b) the results of an in vitro experiment comparing the role of microtubules in controlling cell expansion in different zones near the apex of three types of cotton fiber tips. Adding the common herbicide fluridone, 1-Methyl-3-phenyl-5-[3-(trifluoromethyl)phenyl]-4(1H)-pyridinone, to the medium supported G. barbadense ovule culture, with positive impacts on the number of useful ovules and fiber length. The effect is potentially mediated through inhibited synthesis of abscisic acid, which antagonized the positive effects of fluridone. Fiber development was perturbed by adding colchicine, a microtubule antagonist, to ovules of G. barbadense and G. hirsutum cultured 2 days after flowering. The results supported the zonal control of cell expansion in one type of G. hirsutum fiber tip and highlighted differences in the role of microtubules in modulating cell expansion between three types of cotton fiber tips.}, number={5}, journal={Planta}, publisher={Society for Mining, Metallurgy and Exploration Inc.}, author={Pierce, Ethan T. and Graham, Benjamin P. and Stiff, Michael R. and Osborne, Jason A. and Haigler, Candace H.}, year={2019}, month={Feb}, pages={1551–1563} }
 @article{stiff_haigler_2016, title={Cotton fiber tips have diverse morphologies and show evidence of apical cell wall synthesis}, volume={6}, ISSN={["2045-2322"]}, DOI={10.1038/srep27883}, abstractNote={Abstract}, journal={SCIENTIFIC REPORTS}, author={Stiff, Michael R. and Haigler, Candace H.}, year={2016}, month={Jun} }
 @inbook{stiff_tuttle_graham_haigler_2016, place={Cham, Switzerland}, series={Sustainable Development and Biodiversity}, title={Cotton Fiber Biotechnology: Potential Controls and Transgenic Improvement of Elongation and Cell Wall Thickening}, volume={13}, ISBN={9783319445694 9783319445700}, ISSN={2352-474X 2352-4758}, url={http://dx.doi.org/10.1007/978-3-319-44570-0_8}, DOI={10.1007/978-3-319-44570-0_8}, abstractNote={Cotton is grown on five continents as an economically important crop. Its long, fine, seed fibers are one of the most highly used natural fibers, providing a high-quality spinnable fiber to the textile industry. The cotton fiberCotton fiber undergoes a complex, staged developmental program, resulting in a single cell that is 1.8–5 cm long with a thick wall composed of about 95 % cellulose. Biotechnological improvements have either directly or indirectly enhanced the fiber properties that are important for spinning, including length, bundle strength, and maturity. These experiments have generally targeted carbohydrate metabolism, cell wall structure, and hormone signaling. In this chapter, we present a brief review of cotton fiber developmentCotton fiber development with a focus on processes affecting elongation and cell wall thickening. We discuss rigorous criteria for evaluating studies on transgenic cotton fiber and mention the challenges of performing such research in the public sector. We highlight selected genetic engineering experiments that have resulted in improved cotton fiber quality Cotton fiber quality and discuss future prospects for use of biotechnology to improve cotton fiberCotton fiber and its competitiveness with synthetic fibers.}, booktitle={Fiber Plants}, publisher={Springer International Publishing}, author={Stiff, Michael R. and Tuttle, J. Rich and Graham, Benjamin P. and Haigler, Candace H.}, editor={Ramawat, K. and Ahuja, M.Editors}, year={2016}, pages={127–153}, collection={Sustainable Development and Biodiversity} }
 @article{stiff_weissinger_danehower_2011, title={Analysis of CoQ(10) in Cultivated Tobacco by a High-Performance Liquid Chromatography-Ultraviolet Method}, volume={59}, ISSN={["0021-8561"]}, DOI={10.1021/jf201130z}, abstractNote={Coenzyme Q (CoQ) is a naturally occurring lipid-soluble quinone that performs multiple functions in all living cells and has become a popular antioxidant supplement, a coadjuvant in the treatment of heart disease, and the object of study for treating neurodegenerative disorders. Although there are many tools for CoQ analysis of microbial and animal samples, there have been relatively few reports of methods for CoQ analysis of green plants. This work describes a method for the routine analysis of coenzyme Q(10) in green leaf tissue of cultivated Nicotiana tabacum (tobacco) using high-performance liquid chromatography (HPLC) with UV detection. The method was applied to the analysis of CoQ(10) in N. tabacum 'KY14' leaves at different stalk positions representing young lanceolate to senescing leaves, and it was found that CoQ(10) increased as leaf position changed down the stalk from 18.69 to 82.68 μg/g fw. The method was also used to observe CoQ(10) in N. tabacum 'NC55' and N. tabacum 'TN90LC' leaves over time, finding that CoQ(10) leaf content remained relatively stable from 3 to 6 weeks but increased in both cultivars at 8 weeks. This method will likely be useful in the analysis of CoQ(10) in the green leaves of other plant species.}, number={17}, journal={JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY}, author={Stiff, Michael R. and Weissinger, Arthur K. and Danehower, David A.}, year={2011}, month={Sep}, pages={9054–9058} }