Ming-Zhu Shi

author keywords: Anthocyanin biosynthesis; Arabidopsis thaliana; Auxins; Metabolic engineering; pap1-D cells; WBM complex; Regulation route

MeSH headings : 2,4-Dichlorophenoxyacetic Acid / pharmacology; Anthocyanins / metabolism; Arabidopsis / drug effects; Arabidopsis / genetics; Arabidopsis / metabolism; Arabidopsis Proteins / genetics; Arabidopsis Proteins / metabolism; Biosynthetic Pathways; Gene Expression Regulation, Plant; Indoleacetic Acids / pharmacology; Light; Metabolic Engineering; Pancreatitis-Associated Proteins; Pigmentation; Plant Growth Regulators / pharmacology; Tissue Culture Techniques; Transcription Factors / genetics; Transcription Factors / metabolism

TL;DR: The data demonstrate that the three auxins regulate anthocyanin biosynthesis in metabolically programmed red cells via altering the expression of transcription factor genes and pathway genes, such as DFR and ANS. (via Semantic Scholar)

author keywords: Arabidopsis thaliana; Anthocyanin biosynthesis; Production of anthocyanin pigmentation 1; Nitrogen

MeSH headings : Anthocyanins / biosynthesis; Arabidopsis / cytology; Arabidopsis / genetics; Arabidopsis / metabolism; Arabidopsis Proteins / metabolism; Basic Helix-Loop-Helix Transcription Factors / metabolism; Biosynthetic Pathways; Gene Expression Regulation, Plant; Genes, Plant; Genetic Variation; Genotype; Nitrogen / metabolism; Pancreatitis-Associated Proteins; Transcription Factors / metabolism

TL;DR: Nitrogen regulation of anthocyanin biosynthesis in red cells undergoes a mechanism by which nitrogen controls the expression of genes encoding both main components of the TTG1–GL3/TT8–PAP1 complex and negative regulators. (via Semantic Scholar)

author keywords: Arabidopsis thaliana; Anthocyanin; Biosynthesis; Cyanin; Metabolic engineering; Microarray analysis

MeSH headings : Anthocyanins / chemistry; Anthocyanins / metabolism; Arabidopsis / cytology; Arabidopsis / genetics; Arabidopsis / metabolism; Arabidopsis Proteins; Biosynthetic Pathways; Chromatography, High Pressure Liquid; Gene Expression Profiling; Gene Expression Regulation, Plant; Genes, Plant / genetics; Genetic Engineering / methods; Genome, Plant / genetics; Pancreatitis-Associated Proteins; Principal Component Analysis; Spectrometry, Mass, Electrospray Ionization; Transcription Factors / genetics; Transcription Factors / metabolism

TL;DR: It is suggested that the difference in gene expression profiles between the two cell lines likely results from cell types, the overexpression of PAP1, and the high metabolic flux toward anthocyanins. (via Semantic Scholar)

author keywords: Anthocyanins; Anthocyanidins; Arabidopsis thaliana; Biosynthesis; Cyanins; Cyanidins; HPLC-ESI-MS; HPLC-TOF-MS; pap1-D-mutant; PAP1; Metabolic profiling; RT-PCR

MeSH headings : Anthocyanins / biosynthesis; Anthocyanins / chemistry; Anthocyanins / metabolism; Arabidopsis / cytology; Arabidopsis / drug effects; Arabidopsis / growth & development; Arabidopsis / radiation effects; Arabidopsis Proteins; Biological Transport / drug effects; Biological Transport / radiation effects; Chromatography, High Pressure Liquid; Culture Media / pharmacology; Gene Expression Regulation, Plant / drug effects; Gene Expression Regulation, Plant / radiation effects; Genes, Dominant / genetics; Genes, Plant / genetics; Glycosylation / drug effects; Glycosylation / radiation effects; Light; Mass Spectrometry; Metabolic Networks and Pathways / drug effects; Metabolic Networks and Pathways / radiation effects; Pancreatitis-Associated Proteins; Pigmentation / drug effects; Pigmentation / genetics; Pigmentation / radiation effects; Plant Leaves / drug effects; Plant Leaves / metabolism; Plant Leaves / radiation effects; RNA, Messenger / genetics; RNA, Messenger / metabolism; Transcription Factors / genetics; Transcription Factors / metabolism

TL;DR: The A. thaliana anthocyanin molecule A11 was most likely the first cyanin derived from the multiple modification steps of cyanidin, and rosette leaves of pap1-D plants produced much higher levels and more diverse molecular profiling of cyanins than those of WT plants. (via Semantic Scholar)

author keywords: Anthocyanin; Callus; HPLC-mass spectrum; Metabolic profiling; Nicotiana tabacum; PAP1; Tobacco; Transgene

MeSH headings : 2,4-Dichlorophenoxyacetic Acid / pharmacology; Anthocyanins / biosynthesis; Anthocyanins / chemistry; Arabidopsis / drug effects; Arabidopsis / metabolism; Arabidopsis / radiation effects; Arabidopsis Proteins; Chromatography, High Pressure Liquid; Chromatography, Liquid; Indoleacetic Acids / pharmacology; Light; Mass Spectrometry; Nitrates / pharmacology; Pancreatitis-Associated Proteins; Plants, Genetically Modified; Potassium Compounds / pharmacology; Tissue Culture Techniques; Tobacco / cytology; Tobacco / drug effects; Tobacco / genetics; Tobacco / growth & development; Transcription Factors / metabolism

TL;DR: It is demonstrated that dark, nitrogen nutrients, and auxin apparently affect the anthocyanin profiles in PAP1 transgenic callus cultures; and it is suggested that these cell cultures are an appropriate system to study the regulatory function of P AP1 on the anthOCyanin biosynthesis at post-transcriptional level in vivo. (via Semantic Scholar)