Works (65)
Updated: December 20th, 2024 05:10
2022 review
Mechanisms of coordinating hyaluronan and glycosaminoglycan production by nucleotide sugars
[Review of ]. AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY, 322(6), C1201–C1213.
Contributors: B. Zimmer n, J. Barycki n & n
author keywords: hexosamine biosynthesis pathway; hyaluronan; nucleotide sugars; UDP-glucuronate; UDP-N-acetylglucosamine
MeSH headings : Glycosaminoglycans; Hyaluronan Synthases / genetics; Hyaluronic Acid / metabolism; Nucleotides; Sugars; Uridine Diphosphate N-Acetylglucosamine / metabolism
TL;DR:
Recent structures of several key rate-limiting enzymes in the UDP-sugar synthesis pathways have offered new insights to the overall regulation of hyaluronan production by precursor fate decisions.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
7. Affordable and Clean Energy
(OpenAlex)
Sources: Web Of Science, ORCID, NC State University Libraries
Added: June 27, 2022
2021 journal article
Altered glucuronidation deregulates androgen dependent response profiles and signifies castration resistance in prostate cancer
Oncotarget, 12(19), 1886–1902.
Contributors: B. Zimmer n, M. Howell *, L. Ma n, J. Enders n , D. Lehman n, E. Corey *, J. Barycki n, n
TL;DR:
A role for UGDH in androgen responsiveness and a target for therapeutic strategies in advanced prostate cancer is supported by a comparison of protein and metabolite levels relevant to the glucuronidation pathway in patient-derived xenograft models paired with isogenic counterparts selected in vivo for castration resistant prostate cancer recurrence.
(via Semantic Scholar)
open access via www.oncotarget.com (publisher PDF)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(OpenAlex)
Source: ORCID
Added: July 16, 2022
2020 review
Integration of Sugar Metabolism and Proteoglycan Synthesis by UDP-glucose Dehydrogenase
[Review of ]. JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY, 69(1), 13–23.
Contributors: B. Zimmer n, J. Barycki n & n
author keywords: developmental disorders; extracellular matrix; glucuronidation; glycosaminoglycan; hyaluronan; nucleotide sugars; post-translational modifications; proteoglycan; UDP-glucuronate
MeSH headings : Allosteric Regulation; Animals; Biosynthetic Pathways; Humans; Models, Molecular; Neoplasms / metabolism; Protein Processing, Post-Translational; Proteoglycans / metabolism; Sugars / metabolism; Uridine Diphosphate Glucose Dehydrogenase / chemistry; Uridine Diphosphate Glucose Dehydrogenase / metabolism
TL;DR:
The cellular mechanisms that regulate UG DH expression are reviewed, the structural features of the enzyme are discussed, and the structures are used to provide a context for recent studies that link post-translational modifications and allosteric modulators of UGDH to its function in downstream pathways.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, NC State University Libraries, ORCID
Added: August 24, 2020
2020 journal article
Loss-of-function mutations in UDP-Glucose 6-Dehydrogenase cause recessive developmental epileptic encephalopathy
NATURE COMMUNICATIONS, 11(1).
Contributors: H. Hengel *, C. Bosso-Lefèvre *, G. Grady n, E. Szenker-Ravi *, H. Li *, S. Pierce *, É. Lebigot *, T. Tan*
MeSH headings : Adolescent; Alleles; Animals; Child; Child, Preschool; Epilepsy / genetics; Female; Genes, Recessive; Humans; Infant; Kinetics; Loss of Function Mutation / genetics; Male; Organoids / pathology; Oxidoreductases / chemistry; Oxidoreductases / genetics; Pedigree; Protein Domains; Syndrome; Uridine Diphosphate Glucose Dehydrogenase / genetics; Zebrafish
TL;DR:
Biallelic variants in the gene encoding UDP-Glucose 6-Dehydrogenase (UGDH) in individuals affected by developmental epileptic encephalopathies that impair UGDH stability, oligomerization, or enzymatic activity in vitro are reported.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, NC State University Libraries, ORCID
Added: July 20, 2020
2019 journal article
Catalytically inactive Dnmt3b rescues mouse embryonic development by accessory and repressive functions
NATURE COMMUNICATIONS, 10(1).
Contributors: P. Nowialis *, K. Lopusna *, J. Opavska*, S. Haney*, A. Abraham *, P. Sheng *, A. Riva *, A. Natarajan *
MeSH headings : Animals; Biocatalysis; DNA (Cytosine-5-)-Methyltransferases / genetics; DNA (Cytosine-5-)-Methyltransferases / metabolism; DNA Methylation; Embryonic Development / genetics; Female; Gene Expression Regulation, Developmental; HEK293 Cells; Hedgehog Proteins / genetics; Humans; Mice, Inbred C57BL; Mice, Knockout; Mice, Transgenic; Signal Transduction / genetics; Wnt Proteins / genetics
TL;DR:
It is shown that catalytically inactive Dnmt3b rescues a majority of methylation and expression changes in the absence of DnMT3b during mouse embryonic development and is linked to a control of major developmental pathways, including Wnt and hedgehog signaling.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, NC State University Libraries, ORCID
Added: October 21, 2019
2019 chapter
Hyaluronan pericellular matrix: Particle exclusion assay
In Methods in Molecular Biology (Vol. 1952, pp. 103–110).
UN Sustainable Development Goal Categories
10. Reduced Inequalities
(OpenAlex)
Source: ORCID
Added: December 19, 2024
2018 journal article
Prostate tumor cell exosomes containing hyaluronidase Hyal1 stimulate prostate stromal cell motility by engagement of FAK-mediated integrin signaling
MATRIX BIOLOGY, 78-79, 165–179.
Contributors: C. McAtee*, C. Booth *, C. Elowsky *, L. Zhao *, J. Payne*, T. Fangman*, S. Caplan *, M. Henry *, n
author keywords: Hyaluronan; Hyaluronidase; Prostate cancer; Cell motility; Exosomes; Stromal-epithelial crosstalk
MeSH headings : Autophagosomes / metabolism; Cell Adhesion; Cell Communication; Cell Culture Techniques; Cell Line, Tumor; Cell Movement; Enzyme Activation; Exosomes / metabolism; Focal Adhesion Kinase 1 / metabolism; Gene Expression Regulation, Neoplastic; Humans; Hyaluronoglucosaminidase / metabolism; Integrins / metabolism; Male; Microtubule-Associated Proteins / metabolism; Prostatic Neoplasms / metabolism; Prostatic Neoplasms / pathology; Signal Transduction; Stromal Cells / cytology; Stromal Cells / metabolism; Stromal Cells / pathology; Up-Regulation
TL;DR:
The presence of Hyal1 in tumor-derived exosome and its ability to impact the behavior of stromal cells suggests cell-cell communication via exosomes is a novel mechanism by which elevated Hyal 1 promotes prostate cancer progression.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science; OpenAlex)
Sources: Web Of Science, NC State University Libraries, ORCID
Added: June 17, 2019
2016 journal article
Inhibiting Hexamer Disassembly of Human UDP-Glucose Dehydrogenase by Photoactivated Amino Acid Cross-Linking
Biochemistry, 55(22), 3157–3164.
Contributors: G. Grady*, A. Thelen *, J. Albers*, T. Ju *, J. Guo *, J. Barycki*, *
MeSH headings : Amino Acids / chemistry; Amino Acids / radiation effects; Catalysis; Cross-Linking Reagents; Humans; Kinetics; Light; Models, Molecular; Oxidation-Reduction; Photochemical Processes; Protein Conformation; Protein Multimerization / radiation effects; Uridine Diphosphate Glucose / metabolism; Uridine Diphosphate Glucose Dehydrogenase / chemistry; Uridine Diphosphate Glucose Dehydrogenase / metabolism
TL;DR:
A model for catalysis in which the ability to dissociate the dimer-dimer interface is as important for maximal enzyme function as has been previously shown for the formation of the hexamer is supported.
(via Semantic Scholar)
Sources: Crossref, NC State University Libraries, ORCID
Added: February 24, 2020
2016 journal article
Loss of exogenous androgen dependence by prostate tumor cells is associated with elevated glucuronidation potential
Hormones and Cancer, 7(4), 260–271.
Contributors: B. Zimmer*, M. Howell *, Q. Wei *, L. Ma *, T. Romsdahl*, E. Loughman*, J. Markham *, J. Seravalli *, J. Barycki*, *
author keywords: Prostate cancer; Castration resistance; Dihydrotestosterone; Detoxification; LNCaP
MeSH headings : Androgens / pharmacology; Cell Line, Tumor; Gene Expression Regulation, Neoplastic / drug effects; Glucuronides / metabolism; Glucuronosyltransferase / metabolism; Humans; Kallikreins / genetics; Kallikreins / metabolism; Male; Minor Histocompatibility Antigens / metabolism; Models, Biological; Promoter Regions, Genetic; Prostate-Specific Antigen / genetics; Prostate-Specific Antigen / metabolism; Prostatic Neoplasms / genetics; Prostatic Neoplasms / metabolism; Prostatic Neoplasms, Castration-Resistant / genetics; Prostatic Neoplasms, Castration-Resistant / metabolism; Receptors, Androgen / metabolism; Uridine Diphosphate Glucose Dehydrogenase / metabolism
TL;DR:
A model in which the aberrant partitioning of UDP-glucuronate and other UDP-sugars into alternative pathways during androgen deprivation contributes to the loss of prostate tumor cell androgen sensitivity by promoting altered cell surface proteoglycan expression is supported.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(OpenAlex)
Sources: Crossref, NC State University Libraries, ORCID
Added: February 24, 2020
2015 journal article
Advances in Hyaluronan Biology: Signaling, Regulation, and Disease Mechanisms
International Journal of Cell Biology, 2015.
Contributors: * , C. De La Motte *, L. Sherman * & P. Weigel*
Source: ORCID
Added: December 19, 2024
2015 journal article
Hyaluronidase Hyal1 Increases Tumor Cell Proliferation and Motility through Accelerated Vesicle Trafficking
Journal of Biological Chemistry, 290(21), 13144–13156.
Contributors: C. McAtee*, A. Berkebile*, C. Elowsky *, T. Fangman*, J. Barycki*, J. Wahl*, O. Khalimonchuk *, N. Naslavsky*, S. Caplan *, *
MeSH headings : Adenocarcinoma / metabolism; Adenocarcinoma / pathology; Antigens, Neoplasm / metabolism; Apoptosis; Blotting, Western; Cell Movement; Cell Proliferation; Endocytosis / physiology; Endosomes / metabolism; Histone Acetyltransferases / metabolism; Humans; Hyaluronic Acid / metabolism; Hyaluronoglucosaminidase / metabolism; Male; Prostatic Neoplasms / metabolism; Prostatic Neoplasms / pathology; Protein Transport; Subcellular Fractions; Transferrin / metabolism; Transport Vesicles / metabolism; Tumor Cells, Cultured
TL;DR:
Overall, excess Hyal1 secretion accelerates endocytic vesicle trafficking in a substrate-dependent manner, promoting aggressive tumor cell behavior.
(via Semantic Scholar)
Sources: Crossref, NC State University Libraries, ORCID
Added: February 24, 2020
2015 journal article
Methylation-independent repression of Dnmt3b contributes to oncogenic activity of Dnmt3a in mouse MYC-induced T-cell lymphomagenesis
Oncogene, 34(43), 5436–5446.
Contributors: S. Haney*, R. Hlady *, J. Opavska*, D. Klinkebiel *, S. Pirruccello*, S. Dutta *, K. Datta *, * , L. Wu *, R. Opavsky *
MeSH headings : Animals; Carcinogenesis / genetics; Cell Proliferation / genetics; DNA / genetics; DNA (Cytosine-5-)-Methyltransferases / genetics; DNA Methylation / genetics; DNA Methyltransferase 3A; Disease Models, Animal; Disease Progression; Lymphoma, T-Cell / genetics; Lymphoma, T-Cell / pathology; Mice; Promoter Regions, Genetic / genetics; Proto-Oncogene Proteins c-myc / genetics; Transcriptome / genetics; Up-Regulation / genetics
TL;DR:
The functional consequences of Dnmt3a inactivation in a mouse model of MYC-induced T-cell lymphomagenesis (MTCL) are analyzed to demonstrate an unexpected oncogenic role for Dn MT3b in MTCL through methylation-independent repression of DNmt3b and possibly other tumor suppressor genes.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(OpenAlex)
Sources: Crossref, NC State University Libraries, ORCID
Added: December 21, 2020
2014 chapter
Emerging regulatory paradigms in glutathione metabolism
In Advances in Cancer Research (Vol. 122, pp. 69–101).
Contributors: Y. Liu *, A. Hyde*, * & J. Barycki*
Source: ORCID
Added: December 19, 2024
2014 chapter
Emerging roles for hyaluronidase in cancer metastasis and therapy
In Advances in Cancer Research (Vol. 123, pp. 1–34).
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(OpenAlex)
Source: ORCID
Added: December 19, 2024
2014 book
Source: ORCID
Added: December 19, 2024
2013 journal article
Characterization of IRDye 800CW chlorotoxin as a targeting agent for brain tumors
Analytical Biochemistry, 440(2), 212–219.
Contributors: J. Kovar *, E. Curtis*, S. Othman, * & D. Olive*
author keywords: Medulloblastoma; IRDye 800CW; Chlorotoxin; In vivo imaging; Blood-brain barrier; ND2:SmoA1 homozygous transgenic mouse
MeSH headings : Animals; Benzenesulfonates / chemistry; Blood-Brain Barrier / metabolism; Cell Line, Tumor; Cerebellar Neoplasms / diagnosis; Cerebellar Neoplasms / metabolism; Cerebellar Neoplasms / pathology; Humans; Indoles / chemistry; Matrix Metalloproteinase 2 / metabolism; Medulloblastoma / diagnosis; Medulloblastoma / metabolism; Medulloblastoma / pathology; Mice; Scorpion Venoms / chemistry; Scorpion Venoms / metabolism
TL;DR:
Because increased vascular permeability was observed early in the disease model, larger dye-labeled imaging agents that exceed current BBB size restrictions may warrant renewed consideration as candidates for tumor detection and surgical resection.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(OpenAlex)
Sources: Crossref, NC State University Libraries, ORCID
Added: December 21, 2020
2013 journal article
Essential role for dnmt1 in the prevention and maintenance of MYC-Induced T-Cell lmphomas
Molecular and Cellular Biology, 33(21), 4321–4333.
Contributors: S. Peters*, R. Hlady *, J. Opavska*, D. Klinkebiel *, S. Novakova*, L. Smith *, R. Lewis *, A. Karpf *
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(OpenAlex)
Source: ORCID
Added: December 19, 2024
2013 journal article
Tumor suppressor functions of Dnmt3a and Dnmt3b in the prevention of malignant mouse lymphopoiesis
Leukemia, 28(5), 1138–1142.
Contributors: S. Peters*, R. Hlady *, J. Opavska*, D. Klinkebiel *, S. Pirruccello*, G. Talmon *, J. Sharp*, L. Wu *
MeSH headings : Animals; DNA (Cytosine-5-)-Methyltransferases / metabolism; DNA Methyltransferase 3A; Genes, Tumor Suppressor; Lymphoma / enzymology; Lymphoma / pathology; Lymphopoiesis; Mice
TL;DR:
Tumor suppressor functions of DnMT3a and Dnmt3b in the prevention of malignant mouse lymphopoiesis are inhibited and cell death is prevented in mice treated with F508(B) deletion.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(OpenAlex)
Sources: Crossref, NC State University Libraries, ORCID
Added: December 21, 2020
2013 journal article
UDP-glucose Dehydrogenase Activity and Optimal Downstream Cellular Function Require Dynamic Reorganization at the Dimer-Dimer Subunit Interfaces
Journal of Biological Chemistry, 288(49), 35049–35057.
Contributors: A. Hyde*, A. Thelen *, J. Barycki* & *
author keywords: Dehydrogenase; Enzyme Catalysis; Glycobiology; Hyaluronate; Prostate Cancer; Protein Conformation; Protein Dynamics; Cardiac Defects; Human UGDH; Hyaluronan
MeSH headings : Amino Acid Substitution; Enzyme Stability; HEK293 Cells; Humans; Kinetics; Models, Molecular; Mutagenesis, Site-Directed; Protein Interaction Domains and Motifs; Protein Multimerization; Protein Structure, Quaternary; Protein Subunits; Proteolysis; Thermodynamics; Uridine Diphosphate Glucose Dehydrogenase / chemistry; Uridine Diphosphate Glucose Dehydrogenase / genetics; Uridine Diphosphate Glucose Dehydrogenase / metabolism
TL;DR:
Comparison of kinetic and thermal stability parameters revealed structurally dependent properties consistent with a role for controlled assembly and disassembly of the hexamer in the regulation of UGDH, which support a model that requires an operational dimer-hexamer equilibrium to function efficiently and preserve regulated activity in the cell.
(via Semantic Scholar)
Sources: Crossref, NC State University Libraries, ORCID
Added: August 28, 2020
2012 journal article
Hyaluronan turnover and hypoxic brown adipocytic differentiation are co-localized with ossification in calcified human aortic valves
Pathology - Research and Practice, 208(11), 642–650.
Contributors: E. Stephens *, J. Saltarrelli Jr., L. Balaoing*, L. Baggett *, I. Nandi*, K. Anderson*, J. Morrisett*, M. Reardon *
author keywords: Brown adipocytes; Hyaluronan; Hypoxia; Calcification; Aortic valve
MeSH headings : Adipocytes, Brown / metabolism; Adipocytes, Brown / pathology; Aged; Aortic Valve / metabolism; Aortic Valve / pathology; Aortic Valve / surgery; Aortic Valve Stenosis / metabolism; Aortic Valve Stenosis / pathology; Biomarkers / metabolism; Calcinosis / etiology; Calcinosis / metabolism; Calcinosis / pathology; Cell Adhesion Molecules / metabolism; Cell Differentiation; Female; Glucuronosyltransferase / metabolism; Homeostasis / physiology; Humans; Hyaluronan Synthases; Hyaluronic Acid / metabolism; Hyaluronoglucosaminidase / metabolism; Hypoxia / metabolism; Male; Ossification, Heterotopic / metabolism; Ossification, Heterotopic / pathology
TL;DR:
Roles for hyaluronan homeostasis and the promotion of hypoxia by cells demonstrating brown fat markers in calcific aortic valve disease are suggested.
(via Semantic Scholar)
Sources: Crossref, NC State University Libraries, ORCID
Added: January 30, 2021
2012 journal article
Proline dehydrogenase is essential for proline protection against hydrogen peroxide-induced cell death
Free Radical Biology and Medicine, 53(5), 1181–1191.
Contributors: S. Natarajan *, W. Zhu *, X. Liang *, L. Zhang *, A. Demers *, M. Zimmerman *, * , D. Becker *
Source: ORCID
Added: December 19, 2024
2012 journal article
Systemic blockade of the hyaluronan receptor for endocytosis prevents lymph node metastasis of prostate cancer
International Journal of Cancer, 131(5), E836–E840.
author keywords: prostate cancer; hyaluronan; metastasis; HARE; Stab2; Stabilin 2; mouse orthotopic model
MeSH headings : Adenocarcinoma / immunology; Adenocarcinoma / prevention & control; Adenocarcinoma / secondary; Animals; Antibodies, Monoclonal / therapeutic use; Cell Adhesion Molecules, Neuronal / antagonists & inhibitors; Cell Adhesion Molecules, Neuronal / immunology; Endocytosis; Humans; Hyaluronic Acid / metabolism; Lymph Nodes / drug effects; Lymph Nodes / metabolism; Lymph Nodes / pathology; Lymphatic Metastasis; Male; Mice; Mice, Inbred NOD; Mice, SCID; Prostatic Neoplasms / immunology; Prostatic Neoplasms / pathology; Prostatic Neoplasms / prevention & control; Tumor Cells, Cultured
TL;DR:
The results implicate HARE for the first time in potentiation of tumor metastasis and suggest a novel mechanism by which tumor cell‐associated HA could promote tissue‐specific dissemination.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(OpenAlex)
Sources: Crossref, NC State University Libraries, ORCID
Added: January 30, 2021
2012 journal article
UDP-glucose Dehydrogenase Polymorphisms from Patients with Congenital Heart Valve Defects Disrupt Enzyme Stability and Quaternary Assembly
Journal of Biological Chemistry, 287(39), 32708–32716.
Contributors: A. Hyde*, E. Farmer*, K. Easley*, K. Van Lammerens, V. Christoffels *, J. Barycki*, J. Bakkerss, *
MeSH headings : Amino Acid Substitution; Animals; Animals, Genetically Modified / embryology; Animals, Genetically Modified / genetics; Enzyme Stability / genetics; Escherichia coli; Heart Defects, Congenital / embryology; Heart Defects, Congenital / enzymology; Heart Defects, Congenital / genetics; Heart Valve Diseases / embryology; Heart Valve Diseases / enzymology; Heart Valve Diseases / genetics; Heart Valves / embryology; Heart Valves / enzymology; Muscle Proteins / genetics; Muscle Proteins / metabolism; Mutation, Missense; Polymorphism, Genetic; Protein Structure, Quaternary; Recombinant Proteins / genetics; Recombinant Proteins / metabolism; Uridine Diphosphate Glucose Dehydrogenase; Zebrafish / embryology; Zebrafish / genetics; Zebrafish Proteins / genetics; Zebrafish Proteins / metabolism
TL;DR:
UGDH loss of function mutations result in a subset of human congenital cardiac valve defects caused by reduced enzyme activity during morphogenesis, consistent with the reduced function of both missense mutations significantly reducing the ability of UGDH to provide precursors for cardiac cushion formation, which is essential to subsequent valve formation.
(via Semantic Scholar)
Sources: Crossref, NC State University Libraries, ORCID
Added: August 28, 2020
2011 journal article
Enzymatic Defects Underlying Hereditary Glutamate Cysteine Ligase Deficiency Are Mitigated by Association of the Catalytic and Regulatory Subunits
Biochemistry, 50(29), 6508–6517.
Contributors: M. Willis*, Y. Liu *, E. Biterova *, * , H. Kim *, J. Lee *, J. Barycki*
MeSH headings : Animals; Catalytic Domain; Enzyme Stability; Glutamate-Cysteine Ligase / chemistry; Glutamate-Cysteine Ligase / deficiency; Glutamate-Cysteine Ligase / metabolism; Glutathione / biosynthesis; Homozygote; Humans; Kinetics; Mice; Models, Biological; Mutant Proteins / metabolism; Mutation / genetics; Protein Binding; Protein Structure, Secondary; Recombinant Proteins / metabolism; Saccharomyces cerevisiae / metabolism; Structural Homology, Protein; Temperature
TL;DR:
Kinetic characterizations of the recombinant GCLC mutants indicated that the Arg127Cys, His370Leu, and Pro414Leu mutants have compromised enzymatic activity that can largely be rescued by the addition of GCLM, suggesting that heterodimer formation is needed for stability in vivo.
(via Semantic Scholar)
Sources: Crossref, NC State University Libraries, ORCID
Added: August 28, 2020
2011 journal article
Hyaluronan suppresses prostate tumor cell proliferation through diminished expression of N-cadherin and aberrant growth factor receptor signaling
Experimental Cell Research, 317(8), 1214–1225.
Contributors: A. Bharadwaj *, N. Goodrich *, C. McAtee*, K. Haferbier*, G. Oakley, J. Wahl, *
author keywords: Hyaluronan; Prostate cancer; Cadherin; Integrin; Cell adhesion; Cell cycle
MeSH headings : Antigens, CD / genetics; Antigens, CD / metabolism; Cadherins / genetics; Cadherins / metabolism; Cell Cycle / physiology; Cell Cycle Proteins / genetics; Cell Cycle Proteins / metabolism; Cell Line, Tumor; Cell Proliferation; Cyclin-Dependent Kinases / antagonists & inhibitors; Cyclin-Dependent Kinases / metabolism; Enzyme Activation; Extracellular Signal-Regulated MAP Kinases / metabolism; Focal Adhesion Protein-Tyrosine Kinases / antagonists & inhibitors; Focal Adhesion Protein-Tyrosine Kinases / metabolism; Humans; Hyaluronic Acid / metabolism; Male; Mitogen-Activated Protein Kinase Kinases / antagonists & inhibitors; Mitogen-Activated Protein Kinase Kinases / metabolism; Prostatic Neoplasms / metabolism; Prostatic Neoplasms / pathology; Proto-Oncogene Proteins c-akt / antagonists & inhibitors; Proto-Oncogene Proteins c-akt / metabolism; Receptors, Growth Factor / genetics; Receptors, Growth Factor / metabolism; Signal Transduction / physiology; beta Catenin / genetics; beta Catenin / metabolism
TL;DR:
Detailed characterization of cell cycle progression revealed that expression of Hyal1 accelerated cell cycle re-entry following synchronization, whereas HAS3 alone delayed entry, consistent with a model in which excess HA synthesis suppresses cell proliferation by promoting homotypic E-cadherin mediated cell-cell adhesion.
(via Semantic Scholar)
Sources: Crossref, NC State University Libraries, ORCID
Added: January 30, 2021
2011 journal article
Loss of Dnmt3b function upregulates the tumor modifier Ment and accelerates mouse lymphomagenesis
Journal of Clinical Investigation, 122(1), 163–177.
Contributors: R. Hlady *, S. Novakova*, J. Opavska*, D. Klinkebiel, S. Peters*, J. Bies*, J. Hannah*, J. Iqbal
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(OpenAlex)
Source: ORCID
Added: December 19, 2024
2011 journal article
Near-infrared-labeled tetracycline derivative is an effective marker of bone deposition in mice
Analytical Biochemistry, 416(2), 167–173.
Contributors: J. Kovar *, X. Xu*, D. Draney*, A. Cupp *, * & D. Michael Olive*
author keywords: IRDye 800CW; Tetracycline derivative; Near-infrared; Molecular imaging; Whole animal imaging; Fluorescence microscopy; Immunofluorescent assay; Mineralization
MeSH headings : Animals; Biomarkers / analysis; Bone Remodeling; Calcification, Physiologic; Cell Line; Disease Models, Animal; Fluoresceins / chemistry; Indoles / chemistry; Mice; Mice, Nude; Microscopy, Fluorescence / methods; Osteoporosis / metabolism; Spectroscopy, Near-Infrared / methods; Tetracycline / analysis; Tetracycline / chemistry; Whole Body Imaging
TL;DR:
Evidence is provided that the NIR-labeled BoneTag agent is effective as a general marker of skeletal features and an indicator of the bone mineralization and remodeling processes.
(via Semantic Scholar)
Sources: Crossref, NC State University Libraries, ORCID
Added: January 30, 2021
2009 journal article
Androgen-stimulated UDP-glucose dehydrogenase expression limits prostate androgen availability without impacting hyaluronan levels
Cancer Research, 69(6), 2332–2339.
Contributors: Q. Wei *, R. Galbenus*, A. Raza*, R. Cerny * & *
Source: ORCID
Added: December 19, 2024
2009 chapter
Hyaluronan Synthesis and Turnover in Prostate Cancer
In Hyaluronan in Cancer Biology (pp. 309–327).
Source: ORCID
Added: December 19, 2024
2009 journal article
Hyaluronidase Activity of Human Hyal1 Requires Active Site Acidic and Tyrosine Residues
Journal of Biological Chemistry, 284(14), 9433–9442.
Contributors: L. Zhang *, A. Bharadwaj *, A. Casper *, J. Barkley*, J. Barycki* & *
MeSH headings : Acids / chemistry; Biocatalysis; Catalytic Domain; Cell Line; Crystallography, X-Ray; Disulfides / chemistry; Glycosylation; Humans; Hyaluronoglucosaminidase / chemistry; Hyaluronoglucosaminidase / genetics; Hyaluronoglucosaminidase / metabolism; Hydrogen-Ion Concentration; Kinetics; Models, Molecular; Mutation / genetics; Oligosaccharides / chemistry; Oligosaccharides / metabolism; Protein Structure, Tertiary; Tyrosine / genetics; Tyrosine / metabolism
TL;DR:
Structural information, site-directed mutagenesis, and steady state enzyme kinetics are used to probe molecular determinants of human Hyal1 function and define key components of Hyal 1 active site catalysis, and structural factors critical for stability.
(via Semantic Scholar)
Sources: Crossref, NC State University Libraries, ORCID
Added: August 28, 2020
2009 journal article
Spontaneous metastasis of prostate cancer is promoted by excess hyaluronan synthesis and processing
American Journal of Pathology, 174(3), 1027–1036.
Contributors: A. Bharadwaj *, J. Kovar *, E. Loughman*, C. Elowsky *, G. Oakley * & *
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(OpenAlex)
Source: ORCID
Added: December 19, 2024
2009 journal article
Udp-glucose dehydrogenase as a novel field-specific candidate biomarker of prostate cancer
International Journal of Cancer, 126(2), 315–327.
Contributors: D. Huang*, G. Casale *, J. Tian *, S. Lele*, V. Pisarev, * , G. Hemstreet III
author keywords: UGDH; biomarker; prostate cancer; tissue proteomics; field disease/effect
MeSH headings : Aged; Biomarkers, Tumor / metabolism; Biopsy; Blotting, Western; Cell Line, Tumor; Humans; Male; Microscopy, Fluorescence; Middle Aged; Prostate / enzymology; Prostate / pathology; Prostatic Neoplasms / diagnosis; Prostatic Neoplasms / enzymology; Prostatic Neoplasms / pathology; Protein Array Analysis / methods; Sensitivity and Specificity; Uridine Diphosphate Glucose Dehydrogenase / metabolism
TL;DR:
The results support the UGDH content in prostatic acini as a novel candidate biomarker that may complement the development of a multi‐biomarker panel for detecting PC within the tumor adjacent field on a histologically normal biopsy specimen.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(OpenAlex)
Sources: Crossref, NC State University Libraries, ORCID
Added: January 30, 2021
2008 journal article
Characterization and performance of a near-infrared 2-deoxyglucose optical imaging agent for mouse cancer models
Analytical Biochemistry, 384(2), 254–262.
Contributors: J. Kovar *, W. Volcheck*, E. Sevick-Muraca *, * & D. Olive*
author keywords: IRDye 800CW; 2-Deoxyglucose; Near infrared; Molecular imaging; Immunofluorescent assay; Fluorescence microscopy; Whole animal imaging
MeSH headings : Animals; Cell Line, Tumor; Deoxyglucose / analogs & derivatives; Deoxyglucose / chemistry; Deoxyglucose / metabolism; Diagnostic Imaging; Fluorescent Dyes / chemistry; Fluoroimmunoassay; Humans; Indoles / chemistry; Male; Mice; Mice, Nude; Microscopy, Fluorescence; Neoplasms / diagnosis; Neoplasms / metabolism; Spectroscopy, Near-Infrared; Xenograft Model Antitumor Assays
TL;DR:
Data indicate that IRDye 800CW 2-DG is a broadly applicable optical imaging agent for in vivo imaging of neoplasms in mice with good signal-to-noise characteristics.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(OpenAlex)
Sources: Crossref, NC State University Libraries, ORCID
Added: January 30, 2021
2008 conference paper
Development of fluorescent contrast agents for optical imaging of mouse disease models
Progress in Biomedical Optics and Imaging - Proceedings of SPIE, 6867.
Contributors: J. Kovar *, * , A. Schutz-Geschwender*, X. Xu*, W. Volcheck*, E. Sevickmuraca *, D. Olive*
Source: ORCID
Added: December 19, 2024
2008 journal article
Hyaluronan and hyaluronidase in genitourinary tumors
Frontiers in Bioscience, 13(15), 5664–5680.
Contributors: * & V. Lokeshwar *
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(OpenAlex)
Source: ORCID
Added: December 19, 2024
2007 journal article
A systematic approach to the development of fluorescent contrast agents for optical imaging of mouse cancer models
Analytical Biochemistry, 367(1), 1–12.
Contributors: J. Kovar *, * , A. Schutz-Geschwender* & D. Olive*
MeSH headings : Animals; Contrast Media; Fluorescence; Fluorescent Dyes; Ligands; Membrane Proteins / metabolism; Mice; Molecular Probe Techniques; Neoplasm Proteins / metabolism; Neoplasms, Experimental / diagnosis; Neoplasms, Experimental / metabolism
TL;DR:
This review outlines some of the general principles that are important when developing and using fluorochrome-labeled optical contrast agents for oncology investigations in animals.
(via Semantic Scholar)
Sources: Crossref, NC State University Libraries, ORCID
Added: January 30, 2021
2007 journal article
Inducible Hyaluronan Production Reveals Differential Effects on Prostate Tumor Cell Growth and Tumor Angiogenesis
Journal of Biological Chemistry, 282(28), 20561–20572.
Contributors: A. Bharadwaj *, K. Rector * & *
MeSH headings : Cell Line, Tumor; Gene Expression Regulation, Enzymologic / genetics; Gene Expression Regulation, Neoplastic / genetics; Glucuronosyltransferase / biosynthesis; Glucuronosyltransferase / genetics; Humans; Hyaluronan Synthases; Hyaluronic Acid / metabolism; Hyaluronoglucosaminidase / biosynthesis; Hyaluronoglucosaminidase / genetics; Male; Neoplasm Invasiveness / genetics; Neoplasm Invasiveness / pathology; Neoplasm Proteins / biosynthesis; Neoplasm Proteins / genetics; Neovascularization, Pathologic / enzymology; Neovascularization, Pathologic / genetics; Neovascularization, Pathologic / pathology; Prostatic Neoplasms / enzymology; Prostatic Neoplasms / genetics; Prostatic Neoplasms / pathology
TL;DR:
Relief from growth suppression in vitro but not in vivo by enzymatic removal of HA effectively uncoupled the respective roles of hyaluronan in growth and angiogenesis, suggesting that growth mediation is less critical to establishment of the tumor than early vascular development.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(OpenAlex)
Sources: Crossref, NC State University Libraries, ORCID
Added: January 30, 2021
2006 journal article
Characterization of Human UDP-Glucose Dehydrogenase Reveals Critical Catalytic Roles for Lysine 220 and Aspartate 280
Biochemistry, 46(2), 369–378.
Contributors: K. Easley*, B. Sommer*, G. Boanca*, J. Barycki* & *
MeSH headings : Amino Acid Substitution; Aspartic Acid / chemistry; Catalytic Domain / genetics; Dimerization; Humans; In Vitro Techniques; Kinetics; Lysine / chemistry; Models, Molecular; Mutagenesis, Site-Directed; Protein Structure, Quaternary; Recombinant Proteins / chemistry; Recombinant Proteins / genetics; Recombinant Proteins / metabolism; Uridine Diphosphate Glucose Dehydrogenase / chemistry; Uridine Diphosphate Glucose Dehydrogenase / genetics; Uridine Diphosphate Glucose Dehydrogenase / metabolism
TL;DR:
Three putative active site residues were identified and gel filtration of this substrate-binding mutant also determined it was a dimer, indicating that hexameric quaternary structure is not critical for catalysis.
(via Semantic Scholar)
Sources: Crossref, NC State University Libraries, ORCID
Added: August 28, 2020
2006 journal article
Concurrent expression of hyaluronan biosynthetic and processing enzymes promotes growth and vascularization of prostate tumors in mice
American Journal of Pathology, 169(1), 247–257.
Source: ORCID
Added: December 19, 2024
2006 journal article
Hyaluronan synthesis and turnover induces metastatic growth
Matrix Biology, 25, S40–S40.
Sources: Crossref, NC State University Libraries
Added: January 30, 2021
2006 journal article
Hyaluronidase expression induces prostate tumor metastasis in an orthotopic mouse model
American Journal of Pathology, 169(4), 1415–1426.
Contributors: J. Kovar *, M. Johnson *, W. Volcheck*, J. Chen * & *
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(OpenAlex)
Source: ORCID
Added: December 19, 2024
2006 journal article
Purification method directly influences effectiveness of an epidermal growth factor-coupled targeting agent for noninvasive tumor detection in mice
Analytical Biochemistry, 361(1), 47–54.
Contributors: J. Kovar *, W. Volcheck*, J. Chen * & *
author keywords: EGF receptor; IRDye 800CW EGF; in-cell western assay; binding affinity; noninvasive; whole animal imaging
MeSH headings : Animals; Carcinoma, Squamous Cell / diagnosis; Carcinoma, Squamous Cell / pathology; Cell Line, Tumor; Chromatography, Gel; Chromatography, High Pressure Liquid; Coloring Agents; Epidermal Growth Factor; ErbB Receptors; Humans; Indicators and Reagents; Mice; Mice, Nude; Mice, SCID; Sensitivity and Specificity; Spectrometry, Fluorescence / methods; Transplantation, Heterologous
TL;DR:
This is the first study to directly correlate targeting agent signal strength in whole cell binding, In-Cell Western, and in vivo near-infrared imaging.
(via Semantic Scholar)
Sources: Crossref, NC State University Libraries, ORCID
Added: January 30, 2021
2004 journal article
Characterization of Human UDP-glucose Dehydrogenase: CYS-276 is required for the second of two successive oxidations
Journal of Biological Chemistry, 279(22), 23590–23596.
Contributors: B. Sommer*, J. Barycki* & *
MeSH headings : Binding Sites; Catalytic Domain / genetics; Cysteine; Humans; Lysine; Oxidation-Reduction; Protein Conformation; Streptococcus pyogenes; Structure-Activity Relationship; Uridine Diphosphate Glucose Dehydrogenase / chemistry; Uridine Diphosphate Glucose Dehydrogenase / genetics; Uridine Diphosphate Glucose Dehydrogenase / metabolism
TL;DR:
Cloned, expressed, and affinity-purified the human UGDH and determined its steady state kinetic parameters, which are consistent with the postulated role of Cys-276 as a catalytic residue and supports its position in the reaction mechanism for the human enzyme.
(via Semantic Scholar)
Sources: Crossref, NC State University Libraries, ORCID
Added: August 28, 2020
2004 journal article
Hyaluronan facilitates invasion of colon carcinoma cells in vitro via interaction with CD44
Cancer Research, 64(13), 4569–4576.
Contributors: H. Kim, M. Wheeler, C. Wilson *, J. Iida*, D. Eng, * , J. McCarthy *, K. Bullard*
Source: ORCID
Added: December 19, 2024
2004 journal article
Melanoma chondroitin sulfate proteoglycan enhances FAK and ERK activation by distinct mechanisms
The Journal of Cell Biology, 165(6), 881–891.
Contributors: J. Yang *, M. Price *, C. Neudauer*, C. Wilson *, S. Ferrone *, H. Xia *, J. Iida*, * , J. McCarthy *
author keywords: melanoma chondroitin sulfate proteoglycan; FAK; integrin; ERK1/2; cell spreading
MeSH headings : Amino Acid Sequence; Cell Line, Tumor; Chondroitin Sulfate Proteoglycans / chemistry; Chondroitin Sulfate Proteoglycans / genetics; Chondroitin Sulfate Proteoglycans / pharmacology; Enzyme Activation / drug effects; Focal Adhesion Kinase 1; Focal Adhesion Protein-Tyrosine Kinases; Humans; Melanoma; Membrane Proteins / chemistry; Membrane Proteins / genetics; Membrane Proteins / pharmacology; Mitogen-Activated Protein Kinase 1 / metabolism; Mitogen-Activated Protein Kinase 3; Mitogen-Activated Protein Kinases / metabolism; Molecular Sequence Data; Protein-Tyrosine Kinases / metabolism; Sequence Alignment; Sequence Homology, Amino Acid
TL;DR:
Results indicate that MCSP may facilitate primary melanoma progression by enhancing the activation of key signaling pathways important for tumor invasion and growth.
(via Semantic Scholar)
Sources: Crossref, NC State University Libraries, ORCID
Added: January 30, 2021
2003 journal article
Hyaluronan synthase-3 is upregulated in metastatic colon carcinoma cells and manipulation of expression alters matrix retention and cellular growth
International Journal of Cancer, 107(5), 739–746.
Contributors: K. Bullard*, H. Kim*, M. Wheeler*, C. Wilson *, C. Neudauer*, * , J. McCarthy *
Source: ORCID
Added: December 19, 2024
2002 journal article
Inhibition of prostate tumor cell hyaluronan synthesis impairs subcutaneous growth and vascularization in immunocompromised mice
American Journal of Pathology, 161(3), 849–857.
Contributors: * , C. Wilson * & J. McCarthy *
Source: ORCID
Added: December 19, 2024
2002 journal article
Manipulation of hyaluronan synthase expression in prostate adenocarcinoma cells alters pericellular matrix retention and adhesion to bone marrow endothelial cells
Journal of Biological Chemistry, 277(12), 10050–10057.
Contributors: * , C. Wilson *, L. Furcht*, A. Spicer*, T. Oegema Jr. & J. McCarthy *
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(OpenAlex)
Source: ORCID
Added: December 19, 2024
2002 journal article
The insulation of genes from external enhancers and silencing chromatin
Proceedings of the National Academy of Sciences of the United States of America, 99(SUPPL. 4), 16433–16437.
Contributors: B. Burgess-Beusse*, C. Farrell *, M. Gaszner*, M. Litt*, V. Mutskov*, F. Recillas-Targa *, * , A. West *, G. Felsenfeld *
Source: ORCID
Added: December 19, 2024
2001 journal article
Correlation between histone lysine methylation and developmental changes at the chicken β-globin locus
Science, 293(5539), 2453–2455.
Contributors: M. Litt*, * , M. Gaszner*, C. Allis * & G. Felsenfeld *
Source: ORCID
Added: December 19, 2024
2001 journal article
Hyaluronan Synthase Elevation in Metastatic Prostate Carcinoma Cells Correlates with Hyaluronan Surface Retention, a Prerequisite for Rapid Adhesion to Bone Marrow Endothelial Cells
Journal of Biological Chemistry, 276(21), 17949–17957.
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(OpenAlex)
Source: ORCID
Added: December 19, 2024
2001 journal article
Melanoma Chondroitin Sulfate Proteoglycan Regulates Matrix Metalloproteinase-dependent Human Melanoma Invasion into Type I Collagen
Journal of Biological Chemistry, 276(22), 18786–18794.
Contributors: J. Iida, D. Pei*, T. Kang, * , M. Herlyn *, L. Furcht, J. McCarthy
Source: ORCID
Added: December 19, 2024
2001 journal article
Transitions in histone acetylation reveal boundaries of three separately regulated neighboring loci
EMBO Journal, 20(9), 2224–2235.
Source: ORCID
Added: December 19, 2024
2000 journal article
GATA zinc finger interactions modulate DNA binding and transactivation
Journal of Biological Chemistry, 275(36), 28157–28166.
Contributors: C. Trainor*, R. Ghirlando * & *
Source: ORCID
Added: December 19, 2024
2000 journal article
Structural and functional conservation at the boundaries of the chicken β-globin domain
EMBO Journal, 19(10), 2315–2322.
Contributors: N. Saitoh *, A. Bell*, F. Recillas-Targa *, A. West *, * , M. Pikaart *, G. Felsenfeld *
UN Sustainable Development Goal Categories
15. Life on Land
(OpenAlex)
Source: ORCID
Added: December 19, 2024
1999 journal article
An insulator element and condensed chromatin region separate the chicken β-globin locus from an independently regulated erythroid-specific folate receptor gene
EMBO Journal, 18(14), 4035–4048.
Contributors: M. Prioleau *, P. Nony*, * & G. Felsenfeld *
Source: ORCID
Added: December 19, 2024
1999 conference paper
Biochemical and biophysical analysis of the intracellular lipid binding proteins of adipocytes
Molecular and Cellular Biochemistry, 192(1-2), 33–40.
Contributors: * , V. LiCata*, N. Coe* & D. Bernlohr *
Source: ORCID
Added: December 19, 2024
1999 journal article
Melanoma chondroitin sulphate proteoglycan regulates cell spreading through Cdc42, Ack-1 and p130<sup>cas</sup>
Nature Cell Biology, 1(8), 507–513.
Contributors: K. Eisenmann *, J. McCarthy *, * , P. Keely*, J. Guan *, K. Tachibana *, L. Lim, E. Manser*, L. Furcht*, J. Iida*
Source: ORCID
Added: December 19, 2024
1999 journal article
Targeted disruption of the adipocyte lipid-binding protein (aP2 protein) gene impairs fat cell lipolysis and increases cellular fatty acid levels
Journal of Lipid Research, 40(5), 967–972. http://www.scopus.com/inward/record.url?eid=2-s2.0-0032895839&partnerID=MN8TOARS
Source: ORCID
Added: December 19, 2024
1998 journal article
Analysis of a series of phenylalanine 57 mutants of the adipocyte lipid- binding protein
Biochemistry, 37(31), 10980–10986.
Contributors: * & D. Bernlohr *
Source: ORCID
Added: December 19, 2024
1997 journal article
Biochemical and crystallographic analyses of a portal mutant of the adipocyte lipid-binding protein
Journal of Biological Chemistry, 272(15), 9793–9801.
Contributors: J. Ory *, C. Kane *, * , L. Banaszak* & D. Bernlohr *
UN Sustainable Development Goal Categories
6. Clean Water and Sanitation
(OpenAlex)
Source: ORCID
Added: December 19, 2024
1997 book
Intracellular lipid-binding proteins and their genes
In Annual Review of Nutrition (Vol. 17, pp. 277–303).
UN Sustainable Development Goal Categories
6. Clean Water and Sanitation
(OpenAlex)
Source: ORCID
Added: December 19, 2024
1997 book
Regulation of gene expression in adipose cells by polyunsaturated fatty acids
In Advances in Experimental Medicine and Biology (Vol. 422, pp. 145–156).
Contributors: D. Bernlohr *, N. Coe*, * & A. Hertzel *
Source: ORCID
Added: December 19, 2024
1996 chapter
Chapter 10 Adipose tissue and lipid metabolism
In New Comprehensive Biochemistry (Vol. 31, pp. 257–281).
Source: ORCID
Added: December 19, 2024
1996 journal article
Upregulation of keratinocyte lipid binding protein (KLBP) in fat cells of adipocyte lipid binding protein (aP2) knockout mice
FASEB Journal, 10(6). http://www.scopus.com/inward/record.url?eid=2-s2.0-33749131306&partnerID=MN8TOARS
Source: ORCID
Added: December 19, 2024
Employment
Updated: March 26th, 2022 15:01
2017 - present
North Carolina State University Raleigh, North Carolina, US
Professor and Department Head
Molecular and Structural Biochemistry
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