Mark Thomas Bokhart

Works (9)

Updated: July 5th, 2023 21:17

2017 journal article

IR-MALDESI Mass Spectrometry Imaging at 50 Micron Spatial Resolution

Journal of The American Society for Mass Spectrometry, 28(10), 2099–2107.

By: M. Bokhart n, J. Manni, K. Garrard n, M. Ekelöf n, M. Nazari n & D. Muddiman n 

author keywords: Mass spectrometry imaging; IR-MALDESI; Tissue imaging; Spatial resolution; Laser focusing
TL;DR: This work presents MSI using infrared matrix-assisted laser desorption electrospray ionization (IR-MALDESI) at 50 μm resolution, using an adjustable iris, beam expander, and an aspherical focusing lens to reduce tissue ablation diameters for MSI at high resolution. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Sources: Web Of Science, Crossref, NC State University Libraries
Added: August 6, 2018

2016 journal article

Analysis of Antiretrovirals in Single Hair Strands for Evaluation of Drug Adherence with Infrared-Matrix-Assisted Laser Desorption Electrospray Ionization Mass Spectrometry Imaging

ANALYTICAL CHEMISTRY, 88(2), 1336–1344.

By: E. Rosen *, C. Thompson*, M. Bokhart n, H. Prince*, C. Sykes *, D. Muddiman n , A. Kashuba *

MeSH headings : Anti-Retroviral Agents / analysis; Hair / chemistry; Infrared Rays; Molecular Structure; Optical Imaging; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
TL;DR: Evaluated the detectability of HIV antiretrovirals in hair from a range of drug classes using infrared matrix-assisted laser desorption electrospray ionization (IR-MALDESI) mass spectrometry imaging (MSI) with 100 μm resolution and an MSI method to quantify the melanin biomarker pyrrole-2,3,5-tricarboxylic acid (PTCA) was evaluated. (via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being (Web of Science; OpenAlex)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2016 review

Infrared matrix-assisted laser desorption electrospray ionization mass spectrometry imaging analysis of biospecimens

[Review of ]. ANALYST, 141(18), 5236–5245.

By: M. Bokhart n & D. Muddiman n 

MeSH headings : Histocytological Preparation Techniques; Spectrometry, Mass, Electrospray Ionization; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
TL;DR: Challenges and limitations of MSI using IR-MALDESI are addressed in the context of the bioimaging field and valuable information is provided for targeted, label-free drug distribution studies and untargeted metabolomic datasets. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2016 journal article

Whole-body mass spectrometry imaging by infrared matrix-assisted laser desorption electrospray ionization (IR-MALDESI)

Jove-Journal of Visualized Experiments, (109).

By: M. Nazari, M. Bokhart & D. Muddiman 

Source: NC State University Libraries
Added: August 6, 2018

2015 journal article

Influence of C-Trap Ion Accumulation Time on the Detectability of Analytes in IR-MALDESI MSI

ANALYTICAL CHEMISTRY, 87(20), 10483–10490.

By: E. Rosen n, M. Bokhart n, M. Nazari n & D. Muddiman n 

MeSH headings : Ions / chemistry; Lasers; Spectrometry, Mass, Electrospray Ionization; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; Spectrophotometry, Infrared; Time Factors; Xenobiotics / analysis
TL;DR: Reducing the C-trap ion accumulation time resulted in increased ion abundance by up to 3 orders of magnitude for analytes ranging from xenobiotics to endogenous lipids, and facilitated the reduction of voxel-to-voxel variability by more than 3-fold. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2015 journal article

Influence of Desorption Conditions on Analyte Sensitivity and Internal Energy in Discrete Tissue or Whole Body Imaging by IR-MALDESI

Journal of The American Society for Mass Spectrometry, 26(6), 899–910.

By: E. Rosen n, M. Bokhart n, H. Ghashghaei  n & D. Muddiman n 

author keywords: IR-MALDESI; Mass spectrometry imaging; Laser desorption whole body imaging; Lipids; Xenobiotics
MeSH headings : Anesthetics, Local / analysis; Anesthetics, Local / pharmacokinetics; Animals; Cocaine / analysis; Cocaine / pharmacokinetics; Infrared Rays; Lipid Metabolism; Lipids / analysis; Mice; Spectrometry, Mass, Electrospray Ionization / methods; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods; Whole Body Imaging / methods
TL;DR: Optimized desorption conditions have been used to conduct an untargeted lipidomic analysis of whole body sagittal sections of neonate mouse, with enhanced lipid coverage received by varying the laser wavelength used for mass spectrometry imaging (MSI). (via Semantic Scholar)
UN Sustainable Development Goal Categories
7. Affordable and Clean Energy (OpenAlex)
Sources: Web Of Science, Crossref, NC State University Libraries
Added: August 6, 2018

2015 journal article

Mass Spectrometry Imaging Reveals Heterogeneous Efavirenz Distribution within Putative HIV Reservoirs

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 59(5), 2944–2948.

By: C. Thompson*, M. Bokhart n, C. Sykes *, L. Adamson*, Y. Fedoriw*, P. Luciw*, D. Muddiman n , A. Kashuba *, E. Rosen n

MeSH headings : Alkynes; Animals; Anti-Retroviral Agents / pharmacokinetics; Benzoxazines / pharmacokinetics; Cyclopropanes; Gray Matter / metabolism; Lymph Nodes / metabolism; Macaca; Mass Spectrometry / methods
TL;DR: Intratissue efavirenz distribution was heterogeneous, with the drug concentrating in the lamina propria of the colon, the primary follicles of lymph nodes, and the brain gray matter, the first imaging data of an antiretroviral drug in active viral reservoirs. (via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being (Web of Science; OpenAlex)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2015 journal article

Quantitative mass spectrometry imaging of emtricitabine in cervical tissue model using infrared matrix-assisted laser desorption electrospray ionization

ANALYTICAL AND BIOANALYTICAL CHEMISTRY, 407(8), 2073–2084.

By: M. Bokhart n, E. Rosen n, C. Thompson*, C. Sykes *, A. Kashuba * & D. Muddiman n 

author keywords: Mass spectrometry imaging; IR-MALDESI; Absolute quantification; Drug distribution; HIV; Selected reaction monitoring
MeSH headings : Diagnostic Imaging / instrumentation; Diagnostic Imaging / methods; Female; Humans; Papillomavirus Vaccines / chemistry; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / instrumentation; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods; Uterine Cervical Neoplasms / chemistry; Uterine Cervical Neoplasms / diagnosis
TL;DR: A quantitative mass spectrometry imaging technique using infrared matrix-assisted laser desorption electrospray ionization (IR-MALDESI) is demonstrated for the antiretroviral (ARV) drug emtricitabine in incubated human cervical tissue, leading to a gain in sensitivity and removal of interferences for several ARV drugs. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2014 journal article

Mapping Antiretroviral Drugs in Tissue by IR-MALDESI MSI Coupled to the Q Exactive and Comparison with LC-MS/MS SRM Assay

JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY, 25(12), 2038–2047.

By: J. Barry n, G. Robichaud n, M. Bokhart n, C. Thompson*, C. Sykes *, A. Kashuba *, D. Muddiman n 

author keywords: IR-MALDESI; Mass spectrometry imaging; LC-MS/MS; Drug distribution
MeSH headings : Adenine / analogs & derivatives; Adenine / analysis; Adenine / chemistry; Adenine / pharmacokinetics; Anti-Retroviral Agents / analysis; Anti-Retroviral Agents / chemistry; Anti-Retroviral Agents / pharmacokinetics; Cervix Uteri / chemistry; Cervix Uteri / metabolism; Chromatography, Liquid / methods; Deoxycytidine / analogs & derivatives; Deoxycytidine / analysis; Deoxycytidine / chemistry; Deoxycytidine / pharmacokinetics; Emtricitabine; Female; Histocytochemistry; Humans; Molecular Imaging / methods; Organophosphonates / analysis; Organophosphonates / chemistry; Organophosphonates / pharmacokinetics; Pyrrolidinones / analysis; Pyrrolidinones / chemistry; Pyrrolidinones / pharmacokinetics; Raltegravir Potassium; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods; Tandem Mass Spectrometry / methods; Tenofovir; Tissue Distribution
TL;DR: An agreement between the average intensities from the imaging experiment and the absolute quantities for each drug was observed and this correlation between these two techniques serves as a prerequisite to quantitative IR-MALDESI MSI. (via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being (Web of Science; OpenAlex)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

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