@article{vanblunk_srikanth_pandit_kuznetsov_brudno_2023, title={Absorption rate governs cell transduction in dry macroporous scaffolds}, volume={1}, ISSN={["2047-4849"]}, DOI={10.1039/d2bm01753a}, abstractNote={Dry, macroporous scaffolds efficiently transduce T cells, but the mechanism for this transduction has not been studied previously. We report that liquid volume and resultant differences in liquid absorption rates governs cell transduction efficiency.}, journal={BIOMATERIALS SCIENCE}, author={VanBlunk, Madelyn and Srikanth, Vishal and Pandit, Sharda S. and Kuznetsov, Andrey V. and Brudno, Yevgeny}, year={2023}, month={Jan} }
 @article{vanblunk_agarwalla_pandit_brudno_2022, title={Fabrication and Use of Dry Macroporous Alginate Scaffolds for Viral Transduction of T Cells}, volume={9}, ISSN={["1940-087X"]}, DOI={10.3791/64036}, abstractNote={Genetic engineering of T cells for CAR-T cell therapy has come to the forefront of cancer treatment over the last few years. CAR-T cells are produced by viral gene transfer into T cells. The current gold standard of viral gene transfer involves spinoculation of retronectin-coated plates, which is expensive and time-consuming. There is a significant need for efficient and cost-effective methods to generate CAR-T cells. Described here is a method for fabricating inexpensive, dry macroporous alginate scaffolds, known as Drydux scaffolds, that efficiently promote viral transduction of activated T cells. The scaffolds are designed to be used in place of gold standard spinoculation of retronectin-coated plates seeded with virus and simplify the process for transducing cells. Alginate is cross-linked with calcium-D-gluconate and frozen overnight to create the scaffolds. The frozen scaffolds are freeze-dried in a lyophilizer for 72 h to complete the formation of the dry macroporous scaffolds. The scaffolds mediate viral gene transfer when virus and activated T cells are seeded together on top of the scaffold to produce genetically modified cells. The scaffolds produce >85% primary T cell transduction, which is comparable to the transduction efficiency of spinoculation on retronectin-coated plates. These results demonstrate that dry macroporous alginate scaffolds serve as a cheaper and more convenient alternative to the conventional transduction method.}, number={187}, journal={JOVE-JOURNAL OF VISUALIZED EXPERIMENTS}, author={VanBlunk, Madelyn and Agarwalla, Pritha and Pandit, Sharda and Brudno, Yevgeny}, year={2022}, month={Sep} }