@article{wang_yuan_2016, title={Modeling bubble dynamics and radical kinetics in ultrasound induced microalgal cell disruption}, volume={28}, ISSN={["1873-2828"]}, DOI={10.1016/j.ultsonch.2015.06.025}, abstractNote={Microalgal cell disruption induced by acoustic cavitation was simulated through solving the bubble dynamics in an acoustical field and their radial kinetics (chemical kinetics of radical species) occurring in the bubble during its oscillation, as well as calculating the bubble wall pressure at the collapse point. Modeling results indicated that increasing ultrasonic intensity led to a substantial increase in the number of bubbles formed during acoustic cavitation, however, the pressure generated when the bubbles collapsed decreased. Therefore, cumulative collapse pressure (CCP) of bubbles was used to quantify acoustic disruption of a freshwater alga, Scenedesmus dimorphus, and a marine alga, Nannochloropsis oculata and compare with experimental results. The strong correlations between CCP and the intracellular lipid fluorescence density, chlorophyll-a fluorescence density, and cell particle/debris concentration were found, which suggests that the developed models could accurately predict acoustic cell disruption, and can be utilized in the scale up and optimization of the process.}, journal={ULTRASONICS SONOCHEMISTRY}, author={Wang, Meng and Yuan, Wenqiao}, year={2016}, month={Jan}, pages={7–14} } @article{jia_wang_yuan_shah_shi_meng_ju_yang_2016, title={N2O EMISSION AND NITROGEN TRANSFORMATION IN CHICKEN MANURE AND BIOCHAR CO-COMPOSTING}, volume={59}, ISSN={["2151-0040"]}, DOI={10.13031/trans.59.11685}, abstractNote={This study examined the effect of biochar addition on nitrous oxide (N2O) emission and nitrogen (N) transformation in co-composting of biochar and chicken manure. Compared with the control (no biochar), addition of 20% biochar resulted in a 59.8% decrease in the major peak of N2O emission. Ammonium (NH4+-N) and nitrate (NO3--N) contents in the final product with 20% biochar addition increased by 67.3% and 66.7%, respectively, compared to the control. Turning frequency (TF), the primary parameter of aeration and temperature control in the biochar-manure co-composting process, was also investigated. Results indicated that less frequent turning (e.g., turning every seven days) promoted NH4+-N and NO3-N retention but increased peak N2O emission by 58.1% compared with daily turning. Overall, biochar can be an ideal bulking agent for stabilizing N-rich materials to minimize N2O emission and, with proper aeration, can enhance nitrogen retention based on this laboratory study.}, number={5}, journal={TRANSACTIONS OF THE ASABE}, author={Jia, X. and Wang, M. and Yuan, W. and Shah, S. and Shi, W. and Meng, X. and Ju, X. and Yang, B.}, year={2016}, pages={1277–1283} } @article{jia_wang_yuan_ju_yang_2016, title={The influence of biochar addition on chicken manure composting and associated methane and carbon dioxide emissions}, volume={11}, DOI={10.15376/biores.11.2.5255-5264}, abstractNote={The effect of biochar addition and turning frequency was examined relative to biochar-chicken manure co-composting and its associated methane (CH4) and carbon dioxide (CO2) emissions. The results demonstrated that biochar addition was more effective in accelerating the composting process, which was indicated by a 5.2% increase in peak pile temperature and a 148% increase in peak CO2 emission with 20% biochar amended-compost, compared with the control that had no biochar. The compost pH increased and moisture content decreased significantly over the whole course of composting with the biochar amendment. The addition of 20% biochar also resulted in a 54.9% decrease in peak CH4 emission compared with the control. More frequent turning (daily vs. every 3 or 7 days) accelerated the composting process and reduced the CH4 emission.}, number={2}, journal={BioResources}, author={Jia, X. Y. and Wang, M. and Yuan, Wenqiao and Ju, X. T. and Yang, B. Z.}, year={2016}, pages={5255–5264} } @article{wang_yuan_hale_2016, title={Three-Dimensional Simulation of Ultrasound-Induced Microalgal Cell Disruption}, volume={178}, ISSN={["1559-0291"]}, DOI={10.1007/s12010-015-1937-z}, abstractNote={The three-dimensional distribution (x, y, and z) of ultrasound-induced microalgal cell disruption in a sonochemical reactor was predicted by solving the Helmholtz equation using a three-dimensional acoustic module in the COMSOL Multiphysics software. The simulated local ultrasound pressure at any given location (x, y, and z) was found to correlate with cell disruption of a freshwater alga, Scenedesmus dimorphus, represented by the change of algal cell particle/debris concentration, chlorophyll-a fluorescence density (CAFD), and Nile red stained lipid fluorescence density (LFD), which was also validated by the model reaction of potassium iodide oxidation (the Weissler reaction). Furthermore, the effect of ultrasound power intensity and processing duration on algal cell disruption was examined to address the limitation of the model.}, number={6}, journal={APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY}, author={Wang, M. and Yuan, W. and Hale, Andy}, year={2016}, month={Mar}, pages={1184–1195} } @article{lin_cooper_wang_adams_genzer_dickey_2015, title={Handwritten, Soft Circuit Boards and Antennas Using Liquid Metal Nanoparticles}, volume={11}, ISSN={["1613-6829"]}, DOI={10.1002/smll.201502692}, abstractNote={Soft conductors are created by embedding liquid metal nanoparticles between two elastomeric sheets. Initially, the particles form an electrically insulating composite. Soft circuit boards can be handwritten by a stylus, which sinters the particles into conductive traces by applying localized mechanical pressure to the elastomeric sheets. Antennas with tunable frequencies are formed by sintering nanoparticles in microchannels.}, number={48}, journal={SMALL}, publisher={Wiley}, author={Lin, Yiliang and Cooper, Christopher and Wang, Meng and Adams, Jacob J. and Genzer, Jan and Dickey, Michael D.}, year={2015}, month={Dec}, pages={6397–6403} } @article{wang_yuan_2015, title={Microalgal Cell Disruption via Ultrasonic Nozzle Spraying}, volume={175}, ISSN={["1559-0291"]}, DOI={10.1007/s12010-014-1350-z}, abstractNote={The objective of this study was to understand the effect of operating parameters, including ultrasound amplitude, spraying pressure, nozzle orifice diameter, and initial cell concentration on microalgal cell disruption and lipid extraction in an ultrasonic nozzle spraying system (UNSS). Two algal species including Scenedesmus dimorphus and Nannochloropsis oculata were evaluated. Experimental results demonstrated that the UNSS was effective in the disruption of microalgal cells indicated by significant changes in cell concentration and Nile red-stained lipid fluorescence density between all treatments and the control. It was found that increasing ultrasound amplitude generally enhanced cell disruption and lipid recovery although excessive input energy was not necessary for best results. The effect of spraying pressure and nozzle orifice diameter on cell disruption and lipid recovery was believed to be dependent on the competition between ultrasound-induced cavitation and spraying-generated shear forces. Optimal cell disruption was not always achieved at the highest spraying pressure or biggest nozzle orifice diameter; instead, they appeared at moderate levels depending on the algal strain and specific settings. Increasing initial algal cell concentration significantly reduced cell disruption efficiency. In all UNSS treatments, the effectiveness of cell disruption and lipid recovery was found to be dependent on the algal species treated.}, number={2}, journal={APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY}, author={Wang, M. and Yuan, W.}, year={2015}, month={Jan}, pages={1111–1122} } @article{wang_yuan_2015, title={Microalgal cell disruption in a high-power ultrasonic flow system}, volume={193}, ISSN={["1873-2976"]}, DOI={10.1016/j.biortech.2015.06.040}, abstractNote={A 2-kW continuous ultrasonic flow system (UFS) was found effective in the disruption of two microalgal strains: Scenedesmus dimorphus and Nannochloropsis oculata. Compared to the control, cell debris concentration of UFS treatments increased up to 202% for S. dimorphus and 112% for N. oculata. Similarly, Nile red stained lipid fluorescence density (NRSLD) increased up to 59.5% and 56.3% for S. dimorphus and N. oculata, respectively. It was also found that increasing ultrasound intensity improved cell disruption efficiency indicated by up to 54% increase in NRSLFD of the two strains. Increasing sonication-processing time to 3-min resulted in 33.0% increase for S. dimorphus and 45.7% increase for N. oculata in NRSLFD compared to the control. Cell recirculation was found beneficial to cell disruption, however, higher initial cell concentration significantly reduced cell disruption efficiency, indicated by 98.2% decrease in NRSLFD per cell when initial cell concentration increased from 4.25 × 106 to 1.7 × 107 cells ml−1.}, journal={BIORESOURCE TECHNOLOGY}, author={Wang, Meng and Yuan, Wenqiao}, year={2015}, month={Oct}, pages={171–177} } @article{wang_yuan_jiang_jing_wang_2014, title={Disruption of microalgal cells using high-frequency focused ultrasound}, volume={153}, ISSN={["1873-2976"]}, DOI={10.1016/j.biortech.2013.11.054}, abstractNote={The objective of this study was to evaluate the effectiveness of high-frequency focused ultrasound (HFFU) in microalgal cell disruption. Two microalgal species including Scenedesmus dimorphus and Nannochloropsis oculata were treated by a 3.2-MHz, 40-W focused ultrasound and a 100-W, low-frequency (20kHz) non-focused ultrasound (LFNFU). The results demonstrated that HFFU was effective in the disruption of microalgal cells, indicated by significantly increased lipid fluorescence density, the decrease of cell sizes, and the increase of chlorophyll a fluorescence density after treatments. Compared with LFNFU, HFFU treatment was more energy efficient. The combination of high and low frequency treatments was found to be even more effective than single frequency treatment at the same processing time, indicating that frequency played a critical role in cell disruption. In both HFFU and LFNFU treatments, the effectiveness of cell disruption was found to be dependent on the cell treated.}, journal={BIORESOURCE TECHNOLOGY}, author={Wang, Meng and Yuan, Wenqiao and Jiang, Xiaoning and Jing, Yun and Wang, Zhuochen}, year={2014}, month={Feb}, pages={315–321} }