Works (33)

Updated: April 4th, 2024 23:13

2017 journal article

A VIGS screen identifies immunity in the Arabidopsis Pla-1 accession to viruses in two different genera of the Geminiviridae

PLANT JOURNAL, 92(5), 796–807.

By: M. Reyes n, M. Flores-Vergara, O. Guerra-Peraza n, C. Rajabu*, J. Desai n, Y. Hiromoto-Ruiz n, J. Ndunguru*, L. Hanley-Bowdoin n ...

author keywords: Arabidopsis; BCTV; CaLCuV; geminivirus; gip-1; immunity; Pla-1; VIGS
MeSH headings : Arabidopsis / virology; Geminiviridae / immunology; Gene Silencing; Plant Diseases / immunology; Plant Diseases / virology; Plant Immunity; Quantitative Trait Loci / genetics
TL;DR: The recessive nature of resistance to CaLCuV and the lack of obvious candidate genes near the gip‐1 locus suggest that a novel resistance gene(s) confers immunity. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2015 article

Persistent Virus-Induced Gene Silencing in Asymptomatic Accessions of Arabidopsis

PLANT FUNCTIONAL GENOMICS: METHODS AND PROTOCOLS, 2ND EDITION, Vol. 1284, pp. 305–322.

By: M. Flores n, M. Reyes n, D. Robertson n & S. Kjemtrup*

MeSH headings : Arabidopsis / genetics; Arabidopsis / growth & development; Arabidopsis / virology; Begomovirus / physiology; Cloning, Molecular / methods; Gene Silencing; Gene Targeting / methods; Genetic Vectors / genetics; Host-Pathogen Interactions / genetics
TL;DR: It is shown that genetic variation in the vast collection of Arabidopsis accessions can be leveraged to ameliorate viral symptomology that accompanies the VIGS procedure. (via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger (Web of Science)
13. Climate Action (Web of Science)
Source: Web Of Science
Added: August 6, 2018

2015 chapter

Virus-Induced Gene Silencing of Fiber-Related Genes in Cotton

In Methods in Molecular Biology (Vol. 1287, pp. 219–234).

By: J. Tuttle n, C. Haigler n & D. Robertson n

author keywords: Virus-induced gene silencing; VIGS; Cotton fiber; GFP; Silencing; Semiquantitative RT-PCR
MeSH headings : Agrobacterium / genetics; Agrobacterium / physiology; Agrobacterium / virology; Cotton Fiber; Cotyledon / genetics; Cotyledon / growth & development; Cotyledon / microbiology; Geminiviridae / genetics; Gene Silencing; Gene Transfer Techniques; Genes, Plant; Genetic Vectors / genetics; Gossypium / genetics; Gossypium / growth & development; Gossypium / microbiology; Green Fluorescent Proteins / genetics; Green Fluorescent Proteins / metabolism; Plants, Genetically Modified / growth & development; Plants, Genetically Modified / metabolism; Plants, Genetically Modified / microbiology; Recombinant Proteins / genetics; Recombinant Proteins / metabolism
TL;DR: The use of Green Fluorescent Protein (GFP) is described as a marker for identifying silenced tissues in reproductive tissues, a procedure that requires the use of transgenic plants. (via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger (Web of Science)
13. Climate Action (Web of Science)
Sources: Web Of Science, NC State University Libraries, Crossref
Added: August 6, 2018

2012 journal article

Method: low-cost delivery of the cotton leaf crumple virus-induced gene silencing system

Plant Methods, 8(1), 27.

By: J. Tuttle n, C. Haigler n & D. Robertson n

author keywords: Cotton; VIGS; Virus-induced gene silencing; Cotton leaf crumple virus; Agroinoculation; Particle inflow gun; Bombardment; Gossypium
TL;DR: The adaptation of a virus-induced gene silencing vector for cotton from the bipartite geminivirusCotton leaf crumple virus for delivery by Agrobacterium is described and the construction of two low-cost particle inflow guns are described to extend the versatility of CLCrV-based VIGS for analyzing gene function in cotton. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries, Crossref
Added: August 6, 2018

2011 journal article

An ER-targeted calcium-binding peptide confers salt and drought tolerance mediated by CIPK6 in Arabidopsis

PLANTA, 235(3), 539–552.

By: P. Tsou*, S. Lee n, N. Allen n, H. Winter-Sederoff & D. Robertson n

Contributors: P. Tsou*, S. Lee n, N. Allen n, H. Winter-Sederoff & D. Robertson n

author keywords: ER calcium; Calcium-binding peptide (CBP); CBL-interacting protein kinase (CIPK6); Drought; Salt
MeSH headings : Arabidopsis / drug effects; Arabidopsis / genetics; Arabidopsis / metabolism; Calcium / metabolism; Chlorophyll / metabolism; Droughts; Endoplasmic Reticulum / metabolism; Gene Expression Regulation, Plant; Immunoblotting; Peptides / genetics; Peptides / metabolism; Plant Proteins / genetics; Plant Proteins / metabolism; Plant Roots / drug effects; Plant Roots / genetics; Plant Roots / metabolism; Plants, Genetically Modified / drug effects; Plants, Genetically Modified / genetics; Plants, Genetically Modified / metabolism; Potassium / metabolism; Reverse Transcriptase Polymerase Chain Reaction; Salts / pharmacology; Sodium / metabolism
TL;DR: This work demonstrates that ER (or nuclear) Ca2+ can directly participate in signal transduction to alter gene expression and the discovery of a method for increasing Ca 2+ levels without deleterious effects on plant growth may have practical applications. (via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger (Web of Science)
13. Climate Action (Web of Science)
Sources: Web Of Science, ORCID
Added: August 6, 2018

2008 journal article

Geminivirus-Mediated Gene Silencing from Cotton Leaf Crumple Virus Is Enhanced by Low Temperature in Cotton

Plant Physiology, 148(1), 41–50.

By: J. Tuttle n, A. Idris n, J. Brown n, C. Haigler n & D. Robertson n

MeSH headings : Animals; Capsid Proteins / physiology; Cold Temperature; DNA, Viral / analysis; Flowers / chemistry; Geminiviridae / physiology; Gene Silencing; Genetic Markers; Genetic Vectors / analysis; Genetic Vectors / physiology; Gossypium / chemistry; Gossypium / virology; Host-Pathogen Interactions; Insect Vectors / physiology; Plant Diseases / virology; Plant Leaves / chemistry; Plant Roots / chemistry
TL;DR: The experiments establish the use of the CLCrV silencing vector to study gene function in cotton and show that temperature can have a major impact on the extent of geminivirus-induced gene silencing. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries, Crossref
Added: August 6, 2018

2007 journal article

Geminivirus-induced gene silencing of the tobacco retinoblastoma-related gene results in cell death and altered development

PLANT MOLECULAR BIOLOGY, 65(1-2), 163–175.

By: C. Jordan n, W. Shen n, L. Hanley-Bowdoin n & D. Robertson n

author keywords: DNA VIGS; retinoblastoma-related protein; cell death; geminivirus; hyperplasia; cell differentiation
MeSH headings : Cell Death; DNA, Viral / genetics; Geminiviridae / genetics; Gene Expression Regulation, Plant / genetics; Gene Silencing; Genetic Vectors / genetics; Plant Leaves / genetics; Plant Leaves / growth & development; Plant Leaves / metabolism; Retinoblastoma / genetics; Retinoblastoma / metabolism; Tobacco / cytology; Tobacco / genetics; Tobacco / growth & development
TL;DR: The results suggest that either inappropriate activation of the cell cycle is lethal in plants or that RBR has other functions, unrelated to the cell Cycle, and demonstrate that continual transcription of RBR is necessary for cell survival. (via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger (Web of Science)
3. Good Health and Well-being (OpenAlex)
13. Climate Action (Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2007 journal article

Silencing of the aflatoxin gene cluster in a diploid strain of Aspergillus flavus is suppressed by ectopic aflR expression

GENETICS, 176(4), 2077–2086.

By: C. Smith*, C. Woloshuk*, D. Robertson & G. Payne n

MeSH headings : Aflatoxins / genetics; Aspergillus flavus / genetics; Base Sequence; DNA Primers / genetics; DNA, Fungal / genetics; DNA-Binding Proteins / genetics; Diploidy; Fungal Proteins / genetics; Gene Dosage; Gene Expression; Gene Silencing; Genes, Fungal; Multigene Family; Mutation; Sequence Deletion; Transcription Factors / genetics; Transformation, Genetic
TL;DR: Gene expression analysis revealed a form of reversible silencing based on the position of aflR is likely preventing the aflatoxin genes from being expressed in 649 × wild-type diploids, specific to the a flatoxin gene cluster. (via Semantic Scholar)
Source: Web Of Science
Added: August 6, 2018

2006 journal article

Four plant Dicers mediate viral small RNA biogenesis and DNA virus induced silencing

NUCLEIC ACIDS RESEARCH, 34(21), 6233–6246.

By: T. Blevins*, R. Rajeswaran*, P. Shivaprasad*, D. Beknazariants*, A. Si-Ammour*, H. Park*, F. Vazquez*, D. Robertson n ...

MeSH headings : Arabidopsis / enzymology; Arabidopsis / genetics; Arabidopsis / virology; Arabidopsis Proteins / genetics; Arabidopsis Proteins / metabolism; Caulimovirus / genetics; Geminiviridae / genetics; Gene Silencing; MicroRNAs / metabolism; Mutation; Plant Diseases / virology; Plant Viruses / genetics; RNA, Double-Stranded / metabolism; RNA, Small Interfering / classification; RNA, Small Interfering / metabolism; RNA, Viral / classification; RNA, Viral / metabolism; Ribonuclease III / genetics; Ribonuclease III / metabolism; Tobamovirus / genetics
TL;DR: This work highlights the complexity of virus interaction with host silencing pathways and suggests that DCL multiplicity helps mediate plant responses to diverse viral infections. (via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger (Web of Science)
13. Climate Action (Web of Science)
15. Life on Land (OpenAlex)
Source: Web Of Science
Added: August 6, 2018

2004 journal article

Geminivirus VIGS of endogenous genes requires SGS2/SDE1 and SGS3 and defines a new branch in the genetic pathway for silencing in plants

PLANT JOURNAL, 38(6), 1004–1014.

By: N. Muangsan n, C. Beclin*, H. Vaucheret* & D. Robertson n

author keywords: geminivirus; gene silencing; Arabidopsis; DNA VIGS; RNA-dependent RNA polymerase
MeSH headings : Arabidopsis / genetics; Arabidopsis / virology; Arabidopsis Proteins / genetics; Arabidopsis Proteins / physiology; DNA, Viral / metabolism; Geminiviridae / physiology; Gene Expression Regulation, Plant / physiology; Gene Silencing / physiology; Mutation; Plants, Genetically Modified
TL;DR: It is demonstrated that SGS2/SDE1 and SGS3 are needed for endogenous gene silencing from DNA viruses, and suggest that S GS2/ SDE1 may reduce geminivirus symptoms by targeting viral mRNAs. (via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger (Web of Science)
13. Climate Action (Web of Science)
15. Life on Land (OpenAlex)
Source: Web Of Science
Added: August 6, 2018

2004 patent

Method of suppressing gene expression in plants

Washington, DC: U.S. Patent and Trademark Office.

By: D. Robertson

Source: NC State University Libraries
Added: August 6, 2018

2004 review

Reprogramming plant gene expression: a prerequisite to geminivirus DNA replication

[Review of ]. MOLECULAR PLANT PATHOLOGY, 5(2), 149–156.

By: L. Hanley-Bowdoin*, S. Settlage* & D. Robertson n

TL;DR: Recent experiments showing that geminiviruses also modulate components of the retinoblastoma/E2F transcription regulatory network to induce quiescent plant cells to re-enter the cell cycle and regain the capacity to support high levels of DNA replication are discussed. (via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger (Web of Science)
13. Climate Action (Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2004 patent

Transgenic plants with increased calcium stores

Washington, DC: U.S. Patent and Trademark Office.

By: S. Wyatt, P. Tsou, D. Robertson & W. Boss

Source: NC State University Libraries
Added: August 6, 2018

2004 review

VIGS vectors for gene silencing: Many targets, many tools

[Review of ]. ANNUAL REVIEW OF PLANT BIOLOGY, 55, 495–519.

By: D. Robertson n

author keywords: RNAi; hpRNA; functional genomics; geminiviruses
MeSH headings : Gene Silencing; Genes, Plant / genetics; Genetic Vectors; Genomics; Plants / genetics; Viruses / genetics
TL;DR: This review examines how DNA and RNA viruses have been modified to silence plant gene expression and discusses advantages and disadvantages of VIGS in determining gene function and guidelines for the safe use of viral vectors. (via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger (Web of Science)
13. Climate Action (Web of Science)
Source: Web Of Science
Added: August 6, 2018

2002 journal article

Expression of the high capacity calcium-binding domain of calreticulin increases bioavailable calcium stores in plants

TRANSGENIC RESEARCH, 11(1), 1–10.

By: S. Wyatt n, P. Tsou n & D. Robertson n

author keywords: Arabidopsis; calreticulin; calcium-binding; calcium storage; endoplasmic reticulum; environmental stress
MeSH headings : Agrobacterium tumefaciens / genetics; Arabidopsis / genetics; Arabidopsis / growth & development; Biological Availability; Calcium / metabolism; Calcium-Binding Proteins / chemistry; Calcium-Binding Proteins / metabolism; Calreticulin; Genetic Vectors; Green Fluorescent Proteins; Luminescent Proteins / genetics; Phenotype; Plants, Genetically Modified; Ribonucleoproteins / chemistry; Ribonucleoproteins / metabolism; Zea mays / genetics; Zea mays / metabolism
TL;DR: The data suggest that ectopic expression of the calreticulin C-domain increases Ca2+ stores, and that thisCa2+ reserve can be used by the plant in times of stress. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Source: Web Of Science
Added: August 6, 2018

2002 journal article

Geminivirus-based vectors for gene silencing in Arabidopsis

PLANT JOURNAL, 30(1), 107–114.

By: M. Turnage n, N. Muangsan n, C. Peele n & D. Robertson n

author keywords: geminivirus; gene silencing; RNAi; Arabidopsis; cabbage leaf curl virus
MeSH headings : Animals; Arabidopsis / genetics; Caenorhabditis elegans / genetics; Drosophila melanogaster / genetics; Geminiviridae / genetics; Geminiviridae / growth & development; Gene Expression Regulation, Plant; Gene Silencing; Genetic Vectors / genetics; Transfection / methods
TL;DR: A system based on the bipartite geminivirus cabbage leaf curl virus that allows silencing of endogenous genes singly or in combinations in Arabidopsis, and introduction of these vectors into intact plants bypasses transformation and extends the kinds of silencing studies that can be carried out inArabidopsis. (via Semantic Scholar)
Source: Web Of Science
Added: August 6, 2018

2002 journal article

Host DNA replication is induced by geminivirus infection of differentiated plant cells

PLANT CELL, 14(12), 2995–3007.

By: S. Nagara, L. Hanley-Bowdoin n & D. Robertson n

MeSH headings : Aphidicolin / pharmacology; Bromodeoxyuridine / metabolism; Cell Differentiation / genetics; Cell Differentiation / physiology; DNA Replication / drug effects; DNA Replication / genetics; DNA Replication / physiology; DNA, Viral / drug effects; DNA, Viral / metabolism; Geminiviridae / genetics; Geminiviridae / physiology; Hydroxyurea / pharmacology; Immunohistochemistry; In Situ Hybridization; Microscopy, Fluorescence; Tobacco / chemistry; Tobacco / genetics; Tobacco / virology
TL;DR: 5-bromo-2-deoxyuridine incorporation to examine DNA synthesis directly in infected Nicotiana benthamiana plants to determine if viral reprogramming of host replication controls had an impact on host DNA replication and demonstrate that chromosomal DNA is replicated in TGMV-infected cells. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2002 journal article

Mutations in the gravity persistence signal loci in arabidopsis disrupt the perception and/or signal transduction of gravitropic stimuli

PLANT PHYSIOLOGY, 130(3), 1426–1435.

By: S. Wyatt n, A. Rashotte*, M. Shipp*, D. Robertson n & G. Muday*

MeSH headings : Arabidopsis / genetics; Arabidopsis / growth & development; Arabidopsis / physiology; Biological Transport / drug effects; Cold Temperature; Flowering Tops / genetics; Flowering Tops / growth & development; Flowering Tops / physiology; Gravitropism / genetics; Gravitropism / physiology; Gravity Sensing / drug effects; Gravity Sensing / physiology; Indoleacetic Acids / pharmacology; Mutation; Phenotype; Signal Transduction / genetics; Signal Transduction / physiology; Starch / metabolism
TL;DR: Results are consistent with GPS loci affecting an aspect of the gravity signal perception/transduction pathway that occurs after statolith sedimentation, but before auxin transport. (via Semantic Scholar)
Source: Web Of Science
Added: August 6, 2018

2002 journal article

Two E2F elements regulate the proliferating cell nuclear antigen promoter differently during leaf development

PLANT CELL, 14(12), 3225–3236.

MeSH headings : Base Sequence; Binding Sites / genetics; Cell Cycle Proteins; DNA-Binding Proteins; E2F Transcription Factors; E2F1 Transcription Factor; Geminiviridae / genetics; Geminiviridae / physiology; Gene Expression Regulation, Developmental; Gene Expression Regulation, Plant; Molecular Sequence Data; Mutation; Plant Leaves / genetics; Plant Leaves / growth & development; Plant Leaves / virology; Plants, Genetically Modified; Proliferating Cell Nuclear Antigen / genetics; Proliferating Cell Nuclear Antigen / metabolism; Promoter Regions, Genetic / genetics; Protein Binding; Response Elements / genetics; Tobacco / genetics; Tobacco / growth & development; Tobacco / virology; Transcription Factors / genetics; Transcription Factors / metabolism
TL;DR: Examination of the distal element (E2F1) and how it interacts with the E2F2 site to regulate the PCNA promoter demonstrates that both E2f elements contribute to the repression of thePCNA promoter in mature leaves, whereas the E 2F1 site counters the repression activity of the E1F2 element in young leaves. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2002 journal article

When a day makes a difference. Interpreting data from endoplasmic reticulum-targeted green fluorescent protein fusions in cells grown in suspension culture

Plant Physiology, 128(2), 341–344.

By: S. Persson n, J. Love n, P. Tsou n, D. Robertson n, W. Thompson n & W. Boss n

MeSH headings : Calcium / metabolism; Calcium-Binding Proteins / genetics; Calcium-Binding Proteins / metabolism; Calreticulin; Cells, Cultured; Endoplasmic Reticulum / metabolism; Green Fluorescent Proteins; Luminescent Proteins / genetics; Luminescent Proteins / metabolism; Plasmids / genetics; Recombinant Fusion Proteins / genetics; Recombinant Fusion Proteins / metabolism; Ribonucleoproteins / genetics; Ribonucleoproteins / metabolism; Time Factors; Tobacco / cytology; Tobacco / genetics; Tobacco / metabolism
TL;DR: The stability of the self-contained structure of green fluorescent protein (GFP) has made it the most widely utilized fluorescent marker for gene expression and subcellular localization studies. (via Semantic Scholar)
Sources: NC State University Libraries, NC State University Libraries, Crossref
Added: August 6, 2018

2001 journal article

Can simultaneous inhibition of seedling growth and stimulation of rhizosphere bacterial populations provide evidence for phytotoxin transfer from plant residues in the bulk soil to the rhizosphere of sensitive species?

JOURNAL OF CHEMICAL ECOLOGY, 27(4), 807–829.

By: K. Staman, U. Blum*, F. Louws n & D. Robertson

author keywords: allelopathy; bulk-soil and rhizosphere bacteria; Cucumis sativus; Helianthus annuus; Triticum aestivum; Amaranthus retroflexus; phenolic acid mixtures; chlorogenic acid; phytotoxicity
MeSH headings : Amaranthus / chemistry; Amaranthus / physiology; Bacteria; Cucumis sativus / chemistry; Helianthus / chemistry; Phenols / pharmacology; Plant Development; Plant Extracts / pharmacology; Plant Roots / microbiology; Population Dynamics; Soil Microbiology; Triticum / chemistry; Triticum / physiology
TL;DR: It was possible to observe simultaneous inhibition of cucumber seedlings and stimulation of phenolic acid utilizing rhizosphere bacteria, and therefore provide indirect evidence of Phenolic acid transfer from plant residues in the soil to the root surface, which may be useful for phytotoxins that are more unique than phenolic acids. (via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger (Web of Science)
15. Life on Land (Web of Science; OpenAlex)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2001 journal article

Proliferating cell nuclear antigen transcription is repressed through an E2F consensus element and activated by geminivirus infection in mature leaves

PLANT CELL, 13(6), 1437–1452.

By: E. Egelkrout n, D. Robertson & L. Hanley-Bowdoin

MeSH headings : Base Sequence; Binding Sites; Cell Cycle Proteins; Consensus Sequence; DNA, Complementary; DNA, Plant; DNA-Binding Proteins; E2F Transcription Factors; Geminiviridae / genetics; Geminiviridae / physiology; Gene Expression Regulation, Plant; Gene Expression Regulation, Viral; Molecular Sequence Data; Mosaic Viruses / genetics; Mosaic Viruses / physiology; Mutation; Plant Leaves / metabolism; Plant Leaves / virology; Plants, Genetically Modified; Plants, Toxic; Proliferating Cell Nuclear Antigen / genetics; Promoter Regions, Genetic; Tobacco / genetics; Tobacco / virology; Transcription Factors / metabolism; Transcription, Genetic
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2001 journal article

Silencing of a meristematic gene using geminivirus-derived vectors

PLANT JOURNAL, 27(4), 357–366.

By: C. Peele, C. Jordan, N. Muangsan, M. Turnage, E. Egelkrout n, P. Eagle*, L. Hanley-Bowdoin n, D. Robertson

author keywords: Geminiviruses; homology-dependent gene silencing; meristems; functional genomics; plant DNA viruses; magnesium chelatase; proliferating cell nuclear antigen
MeSH headings : Base Sequence; DNA Primers; DNA, Viral / metabolism; Geminiviridae / genetics; Gene Silencing; Genes, Essential; Genetic Vectors; In Situ Hybridization; Meristem / genetics; Tobacco / genetics; Tobacco / virology
TL;DR: The results demonstrate that geminivirus-derived vectors can be used to study genes involved in meristem function in intact plants and show that endogenous gene silencing can be separated from viral infection. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2001 journal article

The Ca2+ status of the endoplasmic reticulum is altered by induction of calreticulin expression in transgenic plants

PLANT PHYSIOLOGY, 126(3), 1092–1104.

By: S. Persson n, S. Wyatt n, J. Love n, W. Thompson n, D. Robertson n & W. Boss n

MeSH headings : Arabidopsis / genetics; Arabidopsis / metabolism; Calcium / metabolism; Calcium-Binding Proteins / biosynthesis; Calreticulin; Cell Line; Endoplasmic Reticulum / metabolism; Heat-Shock Response; Phenotype; Plant Proteins / metabolism; Plants, Genetically Modified; Plants, Toxic; Ribonucleoproteins / biosynthesis; Tobacco / genetics; Tobacco / metabolism; Zea mays / genetics; Zea mays / metabolism
TL;DR: The level of CRT in heat shock-induced CRT transgenic plants correlated positively with the retention of chlorophyll when the plants were transferred from Ca( 2+)-containing medium to Ca(2+)-depleted medium, consistent with the hypothesis that increasingCRT in the ER increases the ERCa(2+) stores and thereby enhances the survival of plants grown in low Ca(1+) medium. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2000 journal article

A geminivirus replication protein interacts with the retinoblastoma protein through a novel domain to determine symptoms and tissue specificity of infection in plants

EMBO JOURNAL, 19(13), 3485–3495.

By: L. Kong n, B. Orozco, J. Roe, S. Nagar, S. Ou, H. Feiler, T. Durfee, A. Miller ...

author keywords: AL1; cell cycle; differentiation; plant DNA virus; pRb
MeSH headings : Antigens, Polyomavirus Transforming / metabolism; Base Sequence; DNA Primers; Geminiviridae / isolation & purification; Geminiviridae / metabolism; Geminiviridae / physiology; Plants / virology; Protein Binding; Retinoblastoma Protein / metabolism; Viral Proteins / metabolism; Virus Replication
TL;DR: The pRBR binding domain of AL1 is mapped between amino acids 101 and 180 and two mutants are identified that are differentially impacted for AL1–pRBR interactions during geminivirus infection of plants. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2000 journal article

Chromosome condensation induced by geminivirus infection of mature plant cells

Journal of Cell Science, 113(7), 1149–1160.

By: H. Bass, S. Nagar, L. Hanley-Bowdoin & D. Robertson

Source: NC State University Libraries
Added: August 6, 2018

2000 journal article

Geminiviruses - Models for plant DNA replication, transcription and cell cycle regulation ([correction to] vol 35, pg 105, 2000)

Critical Reviews in Biochemistry and Molecular Biology, 35(4), U4.

By: L. Hanley-Bowdoin, S. Settlage, B. Orozco, S. Nagar & D. Robertson

Source: NC State University Libraries
Added: August 6, 2018

2000 journal article

Geminiviruses: Models for plant DNA replication, transcription, and cell cycle regulation

Critical Reviews in Biochemistry and Molecular Biology, 35(2), 105–140.

By: L. Hanley-Bowdoin, S. Settlage, B. Orozco, S. Nagar & D. Robertson

Source: NC State University Libraries
Added: August 6, 2018

1999 journal article

Geminiviruses: Models for plant DNA replication, transcription, and cell cycle regulation {review}

Critical Reviews in Plant Sciences, 18(1), 71–106.

By: L. Hanley-Bowdoin n, S. Settlage n, B. Orozco n, S. Nagar n & D. Robertson n

UN Sustainable Development Goal Categories
2. Zero Hunger (Web of Science)
13. Climate Action (Web of Science)
Source: NC State University Libraries
Added: August 6, 2018

1999 journal article

Mapping and expression of a bifunctional thymidylate synthase, dihydrofolate reductase gene from maize

PLANT MOLECULAR BIOLOGY, 41(6), 733–739.

By: K. Cox n, D. Robertson n & R. Fites n

author keywords: bifunctional enzymes; dihydrofolate reductase; DNA synthesis; endoreduplication; maize; thymidylate synthase
MeSH headings : Amino Acid Sequence; Blotting, Northern; Chromosome Mapping; Cloning, Molecular; DNA, Complementary / chemistry; DNA, Complementary / genetics; Gene Expression Regulation, Developmental; Gene Expression Regulation, Enzymologic; Gene Expression Regulation, Plant; Molecular Sequence Data; Multienzyme Complexes / genetics; RNA, Plant / genetics; RNA, Plant / metabolism; Seeds / enzymology; Seeds / genetics; Seeds / growth & development; Sequence Analysis, DNA; Tetrahydrofolate Dehydrogenase / genetics; Thymidylate Synthase / genetics; Tissue Distribution; Zea mays / enzymology; Zea mays / genetics
TL;DR: A bifunctional gene (ZmDHFR-TS) encoding dihydrofolate reductase (DHFR) and thymidylate synthase (TS) was cloned from a Zea mays cDNA library and represents the first DHFR-TCS gene cloning from a monocotyledonous plant. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Source: Web Of Science
Added: August 6, 2018

1999 journal article

Model system for plant cell biology: GFP imaging in living onion epidermal cells

BIOTECHNIQUES, 26(6), 1125-+.

By: A. Scott n, S. Wyatt n, P. Tsou n, D. Robertson n & N. Allen n

MeSH headings : Cloning, Molecular; Gene Expression Regulation, Plant; Green Fluorescent Proteins; Indicators and Reagents; Luminescent Proteins; Models, Biological; Onions / cytology; Onions / genetics; Organelles / physiology; Plant Proteins / genetics; Staining and Labeling / methods; Transformation, Genetic
TL;DR: This paper presents a rapid transient expression system using onion epidermal peels, which allows for testing of new GFP fusion proteins in a living tissue to determine deleterious effects and the ability to express before stable transformants are attempted. (via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger (Web of Science)
13. Climate Action (Web of Science)
Source: Web Of Science
Added: August 6, 2018

1998 journal article

Gene silencing from plant DNA carried by a Geminivirus

PLANT JOURNAL, 14(1), 91–100.

By: S. Kjemtrup n, K. Sampson n, C. Peele n, L. Nguyen n, M. Conkling n, W. Thompson n, D. Robertson n

TL;DR: The results establish that homologous DNA sequences localized in nuclear episomes can modulate the expression of active chromosomal genes. (via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger (Web of Science)
13. Climate Action (Web of Science)
15. Life on Land (OpenAlex)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

1992 journal article

GENETIC-TRANSFORMATION OF NORWAY SPRUCE (PICEA-ABIES (L) KARST) USING SOMATIC EMBRYO EXPLANTS BY MICROPROJECTILE BOMBARDMENT

PLANT MOLECULAR BIOLOGY, 19(6), 925–935.

By: D. Robertson n, A. Weissinger n, R. Ackley n, S. Glover n & R. Sederoff n

author keywords: BETA-GLUCURONIDASE DNA TRANSFER; KANAMYCIN; MICROPROJECTILES; NORWAY SPRUCE; SOMATIC EMBRYOS
MeSH headings : Base Sequence; DNA, Recombinant / genetics; Genetic Techniques; Molecular Sequence Data; Plasmids / genetics; Polymerase Chain Reaction; Transformation, Genetic; Trees / embryology; Trees / genetics
TL;DR: DNA/DNA hybridization of one transformed line provided conclusive evidence of stable integration and showed copy numbers of over 10 plasmid sequences per genome in Norway spruce tissue. (via Semantic Scholar)
UN Sustainable Development Goal Categories
15. Life on Land (Web of Science; OpenAlex)
Source: Web Of Science
Added: August 6, 2018

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