@article{hanne_easter_stangeland-molo_cole_2020, title={A minimally invasive technique for serial intraosseous perfusion measurements in the murine tibia using laser Doppler flowmetry}, volume={7}, ISBN={2215-0161}, url={https://doi.org/10.1016/j.mex.2020.100814}, DOI={10.1016/j.mex.2020.100814}, abstractNote={In biomedical and preclinical research, the current standard method for measuring blood perfusion inside murine bone, radiolabeled microspheres, is a terminal procedure that cannot be used to monitor longitudinal perfusion changes. Laser Doppler flowmetry (LDF) can assess perfusion within the proximal tibial metaphysis of mice in vivo but requires a surgical procedure to place the measurement probe directly onto the bone surface. Sustained inflammation for over a month following this technique was previously reported, and previous studies have used LDF as an endpoint-only procedure. We developed a modified, minimally invasive LDF procedure to measure intraosseous perfusion in the murine tibia without stimulating local or systemic inflammation or inducing gait abnormalities. This modified technique can be used to measure perfusion weekly for up to at least a month in the murine tibia.•Unlike previous endpoint-only techniques, this modified LDF procedure can be performed weekly to monitor serial changes to intraosseous perfusion in the murine tibia•The modified LDF technique utilizes a smaller, more localized incision to minimize invasiveness and speed recovery.}, journal={METHODSX}, publisher={Elsevier BV}, author={Hanne, Nicholas J. and Easter, Elizabeth D. and Stangeland-Molo, Sandra and Cole, Jacqueline H.}, year={2020}, pages={100814} }
 @article{khodjaniyazova_hanne_cole_muddiman_2019, title={Mass spectrometry imaging (MSI) of fresh bones using infrared matrix-assisted laser desorption electrospray ionization (IR-MALDESI)}, volume={11}, ISSN={["1759-9679"]}, url={https://doi.org/10.1039/C9AY01886G}, DOI={10.1039/c9ay01886g}, abstractNote={We report an effective strategy for direct analysis and two-dimensional (2D) matrix-assisted laser desorption electrospray ionization (IR-MALDESI) mass spectrometry imaging (MSI) of mouse bones that underwent no chemical treatments prior to analysis. To unravel the chemistry in bones under near-physiological conditions, we cut a flash-frozen bone in half longitudinally, placed it in a mold facing flat side down, and poured Plaster of Paris on top of and around the bone. After Plaster of Paris had set, the bone with embedding material was removed from the mold, and placed on the IR-MALDESI imaging stage. Plaster of Paris acted as a fixture to keep every spot on the sample surface the same distance away from the laser focus. To demonstrate the feasibility of IR-MALDESI MSI for analyses of unmodified bones, we imaged bones derived from healthy and stroke-affected mice and generated ion heatmaps showing the spatial distribution of putatively annotated features.}, number={46}, journal={ANALYTICAL METHODS}, publisher={Royal Society of Chemistry (RSC)}, author={Khodjaniyazova, Sitora and Hanne, Nicholas J. and Cole, Jacqueline H. and Muddiman, David C.}, year={2019}, month={Dec}, pages={5929–5938} }
 @article{hanne_steward_sessions_thornburg_sheng_cole_2019, title={Stroke Prevents Exercise-Induced Gains in Bone Microstructure But Not Composition in Mice}, volume={141}, ISSN={["1528-8951"]}, DOI={10.1115/1.4045113}, abstractNote={Ischemic stroke induces rapid loss in bone mineral density that is up to 13 times greater than during normal aging, leading to a markedly increased risk of fracture. Little is known about skeletal changes following stroke beyond density loss. In this study, we use a mild-moderate middle cerebral artery occlusion model to determine the effects of ischemic stroke without bedrest on bone microstructure, dynamic bone formation, and tissue composition. Twenty-seven 12-week-old male C57Bl/6J mice received either a stroke or sham surgery and then either received daily treadmill exercise or remained sedentary for 4 weeks. All mice were ambulatory immediately following stroke, and limb coordination during treadmill exercise was unaffected by stroke, indicating similar mechanical loading across limbs for both stroke and sham groups. Stroke did not directly detriment microstructure, but exercise only stimulated adaptation in the sham group, not the stroke group, with increased bone volume fraction and trabecular thickness in the sham distal femoral metaphysis. Stroke differentially decreased cortical area in the distal femoral metaphysis for the affected limb relative to the unaffected limb, as well as endosteal bone formation rate in the affected tibial diaphysis. Although exercise failed to improve bone microstructure following stroke, exercise increased mineral-to-matrix content in stroke but not sham. Together, these results show that stroke inhibits exercise-induced changes to femoral microstructure but not tibial composition, even without changes to gait. Similarly, affected-unaffected limb differences in cortical bone structure and bone formation rate in ambulatory mice show that stroke affects bone health even without bedrest.}, number={12}, journal={JOURNAL OF BIOMECHANICAL ENGINEERING-TRANSACTIONS OF THE ASME}, author={Hanne, Nicholas J. and Steward, Andrew J. and Sessions, Marci R. and Thornburg, Hannah L. and Sheng, Huaxin and Cole, Jacqueline H.}, year={2019}, month={Dec} }
 @inproceedings{hanne_steward_easter_cole_2016, title={Bone blood perfusion increases with diet-induced obesity, associated with trabecular deterioration in mice}, booktitle={Biomedical Engineering Society Annual Meeting}, author={Hanne, N. J. and Steward, A. J. and Easter, E.D. and Cole, J.H.}, year={2016} }
 @inproceedings{hanne_steward_easter_cole_2016, title={Monitoring exercise- and diet-induced changes in tibial blood perfusion with laser doppler flowmetry in mice}, booktitle={International Bone and Mineral Society Annual Herbert Fleisch Workshop}, author={Hanne, N. and Steward, A. and Easter, E. and Cole, J.}, year={2016} }