@article{spriggs_gedon_linder_bizikova_2024, title={Comparison of selected cytomorphological features of canine pemphigus foliaceus and superficial pyoderma}, volume={6}, ISSN={["1365-3164"]}, url={https://doi.org/10.1111/vde.13267}, DOI={10.1111/vde.13267}, abstractNote={Abstract Background Cytological detection of acantholytic keratinocytes (acantholytic cells [AC]) helps to identify canine pemphigus foliaceus (cPF) yet AC also occurs in superficial pyoderma (SP), the main differential diagnosis. Hypothesis/Objectives To compare selected cytomorphological features of cPF and SP and to establish cytological diagnostic criteria that could differentiate cPF from SP. Animals 40 and 51 client‐owned dogs with PF and SP, respectively. Materials and Methods Impression smears from cPF (64), impetigo (40) and exfoliative superficial pyoderma (ESP) (17) samples were stained with Romanowsky stain, randomised, blinded and evaluated by two investigators independently. The entire sample was screened (×500 or ×1000 magnification) for round (AC1), boat (AC2) and raft AC, eosinophils and bacteria. Interobserver agreements were calculated. Results The average number of the 10 highest ×500 fields for AC1 and AC2 was significantly higher in PF than SP ( p < 0.0001; Kruskal–Wallis test). Rafts and eosinophils were more common in PF than SP ( p < 0.0001; chi‐square test), while bacteria were rare in PF (5%; p < 0.0001; chi‐square test). Observations between the experienced and novice investigators were highly correlated. An ROC analysis identified five AC1/×500‐magnification field as a suitable cut‐off value for predicting PF diagnosis. This cut‐off value was tested by two additional investigators, who identified sensitivity of 84%–100%, specificity of 95%–97% and accuracy of 95%–96% for the diagnosis of cPF. Conclusions and Clinical Relevance Criterion‐based impression smear cytological evaluation can provide strong evidence to support the clinical diagnosis. Acantholytic cell morphology varies in cPF and SP, and experience can improve accuracy in cytological differentiation.}, journal={VETERINARY DERMATOLOGY}, author={Spriggs, Tyler S. and Gedon, Natalie K. Y. and Linder, Keith E. and Bizikova, Petra}, year={2024}, month={Jun} }
 @article{gedon_bizikova_olivry_mendoza-kuznetsova_oberkirchner_robertson_linder_2023, title={Histopathological characterisation of trunk-dominant canine pemphigus foliaceus, and comparison with classic facial and insecticide-triggered forms}, volume={6}, ISSN={["1365-3164"]}, url={https://doi.org/10.1111/vde.13174}, DOI={10.1111/vde.13174}, abstractNote={Abstract Background While the clinical features were described recently, the histopathological characterisation of trunk‐dominant canine pemphigus foliaceus (PF) is lacking, and whether it differs from classic facial or insecticide‐triggered PF is unknown. Hypothesis/Objectives This study describes the histopathological findings of trunk‐dominant PF, and compares the results to classic facial and insecticide‐triggered PF. Animals Skin biopsies from 103 dogs with clinically characterised trunk‐dominant (n = 33), classic facial (n = 26) and insecticide‐triggered PF (n = 44) were included. Materials and Methods Histological sections, randomised and blinded, were scored for over 50 morphological parameters of pustules, epidermis, dermis, adnexa and crusts. Intact pustule area and width were measured by digital microscopy. Results In trunk‐dominant PF, 77 intact pustules were predominantly subcorneal (0.0019–1.940 mm 2 area, 0.0470–4.2532 mm wide), and contained from one to over 100 acantholytic keratinocytes. Pustules had boat acantholytic cells, corneocytes, perinuclear eosinophilic rings, neutrophil rosettes, acantholytic cell necrosis, rafts, cling‐ons and/or eosinophils. Peripustular epidermal spongiosis, necrosis and lymphocyte exocytosis occurred, as did follicular pustules. Mixed dermal inflammation often contained eosinophils. Trunk‐dominant PF did not differ from the other PF groups except for few parameters, such as having fewer rafts ( p = 0.003). Additional autoimmune inflammatory patterns occurred in all PF groups. Conclusions and Clinical Relevance Trunk‐dominant PF and other canine PF variants are histologically similar, which indicates shared pathomechanisms. The identification of common boat acantholytic cells and corneocyte separation has implications for the mechanisms of acantholysis. The diversity of histopathological and polyautoimmunity features support complicated immune mechanisms. Finally, results indicate that diagnostic biopsies cannot differentiate between these PF variants in dogs.}, journal={VETERINARY DERMATOLOGY}, author={Gedon, Natalie Katharina Yvonne and Bizikova, Petra and Olivry, Thierry and Mendoza-Kuznetsova, Ekaterina and Oberkirchner, Ursula and Robertson, James Benjamin and Linder, Keith Emerson}, year={2023}, month={Jun} }
 @article{mueller_zablotski_baumann_boehm_kasper_klinger_monke_udraite-vovk_weitzer_gedon_2022, title={A randomised, double-blinded comparison between subcutaneous rush and intralympathic allergen immunotherapy induction in atopic dogs}, ISSN={["1365-3164"]}, DOI={10.1111/vde.13138}, abstractNote={Abstract Background Atopic dermatitis (AD) is one of the most common skin diseases in small animal practice. Allergen immunotherapy (AIT) is the only curative treatment for the disease, and oral, subcutaneous and intralymphatic administration of allergens are commonly employed. Objectives To compare the efficacy of AIT following an induction phase with intralymphatic injections (ILIT) or rush immunotherapy (RIT). Animals Fifty privately owned dogs with AD. Materials and Methods In a double‐blinded study, dogs were randomly assigned to either four monthly ILIT of allergen extract or RIT with five injections administered subcutaneously at hourly intervals on the first day. They were assessed by validated scores; Canine Atopic Dermatitis Lesion Index (CADLI) and pruritus Visual Analog Scale (PVAS) at the beginning of the study and after 1, 3, 6 and 12 months. The latter were performed daily for 7 days before each revisit. Medication scores and a total clinical score were calculated and compared between each group and time point. Results There was no significant difference in CADLI and PVAS scores, or CADLI and medication scores between groups at any of the time points. A significant improvement with both ILIT and RIT was seen in total and pruritus scores, respectively. An owner global assessment of good‐to‐excellent treatment efficacy was seen in 40% of the dogs; total scores improved by 27% and 35% in the RIT and ILIT group, respectively. Adverse effects were not seen. Conclusions and Clinical Relevance Induction of AIT can be conducted either as RIT or ILIT with no loss in efficacy.}, journal={VETERINARY DERMATOLOGY}, author={Mueller, Ralf S. and Zablotski, Yuri and Baumann, Katja and Boehm, Teresa and Kasper, Bettina and Klinger, Christoph and Monke, Maarten and Udraite-Vovk, Laura and Weitzer, Tamara and Gedon, Natalie K. Y.}, year={2022}, month={Nov} }