@article{hoque_mahmood_ali_sefat_huang_petersen_harrington_fang_gluck_2023, title={Development of a Pneumatic-Driven Fiber-Shaped Robot Scaffold for Use as a Complex 3D Dynamic Culture System}, volume={8}, ISSN={["2313-7673"]}, url={https://doi.org/10.3390/biomimetics8020170}, DOI={10.3390/biomimetics8020170}, abstractNote={Cells can sense and respond to different kinds of continuous mechanical strain in the human body. Mechanical stimulation needs to be included within the in vitro culture system to better mimic the existing complexity of in vivo biological systems. Existing commercial dynamic culture systems are generally two-dimensional (2D) which fail to mimic the three-dimensional (3D) native microenvironment. In this study, a pneumatically driven fiber robot has been developed as a platform for 3D dynamic cell culture. The fiber robot can generate tunable contractions upon stimulation. The surface of the fiber robot is formed by a braiding structure, which provides promising surface contact and adequate space for cell culture. An in-house dynamic stimulation using the fiber robot was set up to maintain NIH3T3 cells in a controlled environment. The biocompatibility of the developed dynamic culture systems was analyzed using LIVE/DEAD™ and alamarBlue™ assays. The results showed that the dynamic culture system was able to support cell proliferation with minimal cytotoxicity similar to static cultures. However, we observed a decrease in cell viability in the case of a high strain rate in dynamic cultures. Differences in cell arrangement and proliferation were observed between braided sleeves made of different materials (nylon and ultra-high molecular weight polyethylene). In summary, a simple and cost-effective 3D dynamic culture system has been proposed, which can be easily implemented to study complex biological phenomena in vitro.}, number={2}, journal={BIOMIMETICS}, author={Hoque, Muh Amdadul and Mahmood, Nasif and Ali, Kiran M. and Sefat, Eelya and Huang, Yihan and Petersen, Emily and Harrington, Shane and Fang, Xiaomeng and Gluck, Jessica M.}, year={2023}, month={Jun} }
 @article{suh_twiddy_mahmood_ali_lubna_bradford_daniele_gluck_2022, title={Electrospun Carbon Nanotube-Based Scaffolds Exhibit High Conductivity and Cytocompatibility for Tissue Engineering Applications}, volume={7}, ISSN={["2470-1343"]}, url={https://doi.org/10.1021/acsomega.2c01807}, DOI={10.1021/acsomega.2c01807}, abstractNote={Carbon nanotubes (CNTs) are known for their excellent conductive properties. Here, we present two novel methods, “sandwich” (sCNT) and dual deposition (DD CNT), for incorporating CNTs into electrospun polycaprolactone (PCL) and gelatin scaffolds to increase their conductance. Based on CNT percentage, the DD CNT scaffolds contain significantly higher quantities of CNTs than the sCNT scaffolds. The inclusion of CNTs increased the electrical conductance of scaffolds from 0.0 ± 0.00 kS (non-CNT) to 0.54 ± 0.10 kS (sCNT) and 5.22 ± 0.49 kS (DD CNT) when measured parallel to CNT arrays and to 0.25 ± 0.003 kS (sCNT) and 2.85 ± 1.12 (DD CNT) when measured orthogonally to CNT arrays. The inclusion of CNTs increased fiber diameter and pore size, promoting cellular migration into the scaffolds. CNT inclusion also decreased the degradation rate and increased hydrophobicity of scaffolds. Additionally, CNT inclusion increased Young’s modulus and failure load of scaffolds, increasing their mechanical robustness. Murine fibroblasts were maintained on the scaffolds for 30 days, demonstrating high cytocompatibility. The increased conductivity and high cytocompatibility of the CNT-incorporated scaffolds make them appropriate candidates for future use in cardiac and neural tissue engineering.}, number={23}, journal={ACS OMEGA}, publisher={American Chemical Society (ACS)}, author={Suh, Taylor C. and Twiddy, Jack and Mahmood, Nasif and Ali, Kiran M. and Lubna, Mostakima M. and Bradford, Philip D. and Daniele, Michael A. and Gluck, Jessica M.}, year={2022}, month={Jun}, pages={20006–20019} }
 @article{ali_huang_amanah_mahmood_suh_gluck_2022, title={In Vitro Biocompatibility and Degradation Analysis of Mass-Produced Collagen Fibers}, volume={14}, ISSN={["2073-4360"]}, url={https://doi.org/10.3390/polym14102100}, DOI={10.3390/polym14102100}, abstractNote={Automation and mass-production are two of the many limitations in the tissue engineering industry. Textile fabrication methods such as electrospinning are used extensively in this field because of the resemblance of the extracellular matrix to the fiber structure. However, electrospinning has many limitations, including the ability to mass-produce, automate, and reproduce products. For this reason, this study evaluates the potential use of a traditional textile method such as spinning. Apart from mass production, these methods are also easy, efficient, and cost-effective. This study uses bovine-derived collagen fibers to create yarns using the traditional ring spinning method. The collagen yarns are proven to be biocompatible. Enzymatic biodegradability was also confirmed for its potential use in vivo. The results of this study prove the safety and efficacy of the material and the fabrication method. The material encourages higher cell proliferation and migration compared to tissue culture-treated plastic plates. The process is not only simple but is also streamlined and replicable, resulting in standardized products that can be reproduced.}, number={10}, journal={POLYMERS}, author={Ali, Kiran M. and Huang, Yihan and Amanah, Alaowei Y. and Mahmood, Nasif and Suh, Taylor C. and Gluck, Jessica M.}, year={2022}, month={May} }
 @article{mahmood_suh_ali_sefat_jahan_huang_gilger_gluck_2022, title={Induced Pluripotent Stem Cell-Derived Corneal Cells: Current Status and Application}, volume={8}, ISSN={["2629-3277"]}, url={https://doi.org/10.1007/s12015-022-10435-8}, DOI={10.1007/s12015-022-10435-8}, abstractNote={Deficiency and dysfunction of corneal cells leads to the blindness observed in corneal diseases such as limbal stem cell deficiency (LSCD) and bullous keratopathy. Regenerative cell therapies and engineered corneal tissue are promising treatments for these diseases [1]. However, these treatments are not yet clinically feasible due to inadequate cell sources. The discovery of induced pluripotent stem cells (iPSCs) by Shinya Yamanaka has provided a multitude of opportunities in research because iPSCs can be generated from somatic cells, thus providing an autologous and unlimited source for corneal cells. Compared to other stem cell sources such as mesenchymal and embryonic, iPSCs have advantages in differentiation potential and ethical concerns, respectively. Efforts have been made to use iPSCs to model corneal disorders and diseases, drug testing [2], and regenerative medicine [1]. Autologous treatments based on iPSCs can be exorbitantly expensive and time-consuming, but development of stem cell banks with human leukocyte antigen (HLA)- homozygous cell lines can provide cost- and time-efficient allogeneic alternatives. In this review, we discuss the early development of the cornea because protocols differentiating iPSCs toward corneal lineages rely heavily upon recapitulating this development. Differentiation of iPSCs toward corneal cell phenotypes have been analyzed with an emphasis on feeder-free, xeno-free, and well-defined protocols, which have clinical relevance. The application, challenges, and potential of iPSCs in corneal research are also discussed with a focus on hurdles that prevent clinical translation.}, journal={STEM CELL REVIEWS AND REPORTS}, author={Mahmood, Nasif and Suh, Taylor Cook and Ali, Kiran M. and Sefat, Eelya and Jahan, Ummay Mowshome and Huang, Yihan and Gilger, Brian C. and Gluck, Jessica M.}, year={2022}, month={Aug} }
 @article{mahmood_lee_2021, title={Factors Influencing Older Adults' Acceptance of Health Monitoring Smart Clothing}, ISSN={["1552-3934"]}, DOI={10.1111/fcsr.12404}, abstractNote={We aimed to examine the key factors that influence older adults’ perception and intention to wear health monitoring smart clothing. A convenience sample of 376 U.S. older adults was used for data analyses. Influence of functional and aesthetic attributes on older adults’ perception and intention of its use was not supported. The findings imply that older adults who are satisfied with expressive and tracking attributes of health monitoring smart clothing find it useful, easier to use, and socially acceptable, which leads them to be more likely to use it.}, journal={FAMILY & CONSUMER SCIENCES RESEARCH JOURNAL}, author={Mahmood, Nasif and Lee, Young-A}, year={2021}, month={Jun} }