@article{hetzler_marinakos_lott_mohammad_lass-napiorkowska_kolbe_turrentine_fields_overton_marie_et al._2024, title={Adeno-associated virus genome quantification with amplification-free CRISPR-Cas12a}, volume={3}, ISSN={["1476-5462"]}, DOI={10.1038/s41434-024-00449-x}, journal={GENE THERAPY}, author={Hetzler, Zach and Marinakos, Stella M. and Lott, Noah and Mohammad, Noor and Lass-Napiorkowska, Agnieszka and Kolbe, Jenna and Turrentine, Lauren and Fields, Delaney and Overton, Laurie and Marie, Helena and et al.}, year={2024}, month={Mar} }
 @article{jamalzadegan_kim_mohammad_koduri_hetzler_lee_dickey_wei_2024, title={Liquid Metal-Based Biosensors: Fundamentals and Applications}, volume={1}, ISSN={["1616-3028"]}, url={https://doi.org/10.1002/adfm.202308173}, DOI={10.1002/adfm.202308173}, abstractNote={Abstract}, journal={ADVANCED FUNCTIONAL MATERIALS}, author={Jamalzadegan, Sina and Kim, Sooyoung and Mohammad, Noor and Koduri, Harshita and Hetzler, Zach and Lee, Giwon and Dickey, Michael D. and Wei, Qingshan}, year={2024}, month={Jan} }
 @article{mohammad_talton_dalgan_hetzler_steksova_wei_2024, title={Ratiometric nonfluorescent CRISPR assay utilizing Cas12a-induced plasmid supercoil relaxation}, volume={7}, ISSN={["2399-3669"]}, DOI={10.1038/s42004-024-01214-2}, abstractNote={Most CRISPR-based biosensors rely on labeled reporter molecules and expensive equipment for signal readout. A recent approach quantifies analyte concentration by sizing λ DNA reporters via gel electrophoresis, providing a simple solution for label-free detection. Here, we report an alternative strategy for label-free CRISPR-Cas12a, which relies on Cas12a trans-nicking induced supercoil relaxation of dsDNA plasmid reporters to generate a robust and ratiometric readout. The ratiometric CRISPR (rCRISPR) measures the relative percentage of supercoiled plasmid DNA to the relaxed circular DNA by gel electrophoresis for more accurate target concentration quantification. This simple method is two orders of magnitude more sensitive than the typical fluorescent reporter. This self-referenced strategy solves the potential application limitations of previously demonstrated DNA sizing-based CRISPR-Dx without compromising the sensitivity. Finally, we demonstrated the applicability of rCRISPR for detecting various model DNA targets such as HPV 16 and real AAV samples, highlighting its feasibility for point-of-care CRISPR-Dx applications.}, number={1}, journal={COMMUNICATIONS CHEMISTRY}, author={Mohammad, Noor and Talton, Logan and Dalgan, Selen and Hetzler, Zach and Steksova, Anastasiia and Wei, Qingshan}, year={2024}, month={Jun} }
 @article{weng_you_yang_mohammad_lin_wei_gao_zhang_2023, title={CRISPR-Cas Biochemistry and CRISPR-Based Molecular Diagnostics}, volume={2}, ISSN={["1521-3773"]}, DOI={10.1002/anie.202214987}, abstractNote={Abstract}, journal={ANGEWANDTE CHEMIE-INTERNATIONAL EDITION}, author={Weng, Zhengyan and You, Zheng and Yang, Jie and Mohammad, Noor and Lin, Mengshi and Wei, Qingshan and Gao, Xue and Zhang, Yi}, year={2023}, month={Feb} }
 @article{mohammad_talton_hetzler_gongireddy_wei_2023, title={Unidirectional trans-cleaving behavior of CRISPR-Cas12a unlocks for an ultrasensitive assay using hybrid DNA reporters containing a 3' toehold}, volume={8}, ISSN={["1362-4962"]}, url={https://doi.org/10.1093/nar/gkad715}, DOI={10.1093/nar/gkad715}, abstractNote={Abstract}, journal={NUCLEIC ACIDS RESEARCH}, author={Mohammad, Noor and Talton, Logan and Hetzler, Zach and Gongireddy, Megha and Wei, Qingshan}, year={2023}, month={Aug} }
 @article{mohammad_katkam_wei_2022, title={A Sensitive and Nonoptical CRISPR Detection Mechanism by Sizing Double-Stranded lambda DNA Reporter}, volume={11}, ISSN={["1521-3773"]}, url={https://doi.org/10.1002/anie.202213920}, DOI={10.1002/anie.202213920}, abstractNote={Abstract}, journal={ANGEWANDTE CHEMIE-INTERNATIONAL EDITION}, author={Mohammad, Noor and Katkam, Shrinivas S. and Wei, Qingshan}, year={2022}, month={Nov} }
 @article{mohammad_katkam_wei_2022, title={A Sensitive and Nonoptical CRISPR Detection Mechanism by Sizing Double‐Stranded λ DNA Reporter}, url={https://doi.org/10.1002/ange.202213920}, DOI={10.1002/ange.202213920}, abstractNote={Abstract}, journal={Angewandte Chemie}, author={Mohammad, Noor and Katkam, Shrinivas S. and Wei, Qingshan}, year={2022}, month={Dec} }
 @article{mohammad_katkam_wei_2022, title={Recent Advances in Clustered Regularly Interspaced Short Palindromic Repeats-Based Biosensors for Point-of-Care Pathogen Detection}, volume={7}, ISSN={["2573-1602"]}, url={https://doi.org/10.1089/crispr.2021.0146}, DOI={10.1089/crispr.2021.0146}, abstractNote={Infectious pathogens are pressing concerns due to their heavy toll on global health and socioeconomic infrastructure. Rapid, sensitive, and specific pathogen detection methods are needed more than ever to control disease spreading. The fast evolution of clustered regularly interspaced short palindromic repeats (CRISPR)-based diagnostics (CRISPR-Dx) has opened a new horizon in the field of molecular diagnostics. This review highlights recent efforts in configuring CRISPR technology as an efficient diagnostic tool for pathogen detection. It starts with a brief introduction of different CRISPR-Cas effectors and their working principles for disease diagnosis. It then focuses on the evolution of laboratory-based CRISPR technology toward a potential point-of-care test, including the development of new signaling mechanisms, elimination of preamplification and sample pretreatment steps, and miniaturization of CRISPR reactions on digital assay chips and lateral flow devices. In addition, promising examples of CRISPR-Dx for pathogen detection in various real samples, such as blood, saliva, nasal swab, plant, and food samples, are highlighted. Finally, the challenges and perspectives of future development of CRISPR-Dx for infectious disease monitoring are discussed.}, journal={CRISPR JOURNAL}, author={Mohammad, Noor and Katkam, Shrinivas Siddhivinayak and Wei, Qingshan}, year={2022}, month={Jul} }
 @article{stanciu_wei_barui_mohammad_2021, title={Recent Advances in Aptamer-Based Biosensors for Global Health Applications}, volume={23}, ISSN={["1545-4274"]}, DOI={10.1146/annurev-bioeng-082020-035644}, abstractNote={ Since aptamers were first reported in the early 2000s, research on their use for the detection of health-relevant analytical targets has exploded. This review article provides a brief overview of the most recent developments in the field of aptamer-based biosensors for global health applications. The review provides a description of general aptasensing principles and follows up with examples of recent reports of diagnostics-related applications. These applications include detection of proteins and small molecules, circulating cancer cells, whole-cell pathogens, extracellular vesicles, and tissue diagnostics. The review also discusses the main challenges that this growing technology faces in the quest of bringing these new devices from the laboratory to the market. }, journal={ANNUAL REVIEW OF BIOMEDICAL ENGINEERING, VOL 23, 2021}, author={Stanciu, Lia A. and Wei, Qingshan and Barui, Amit K. and Mohammad, Noor}, year={2021}, pages={433–459} }