Nathaniel Paul Grubbs

Works (14)

Updated: April 24th, 2025 09:10

2023 journal article

A draft Diabrotica virgifera virgifera genome: insights into control and host plant adaption by a major maize pest insect

BMC Genomics, 24(1).

By: B. Coates *, K. Walden *, D. Lata *, N. Vellichirammal *, R. Mitchell *, M. Andersson *, R. McKay*, M. Lorenzen n  ...

author keywords: Genome assembly; Host plant specialization; Differential expression
MeSH headings : Animals; Zea mays / physiology; Coleoptera / genetics; Larva / metabolism; Poaceae / genetics; Insecticides / metabolism; Pest Control, Biological; Plants, Genetically Modified / genetics; Endotoxins
topics (OpenAlex): Insect Resistance and Genetics; CRISPR and Genetic Engineering; Genetics, Aging, and Longevity in Model Organisms
TL;DR: Insight is provided into transcriptomic responses of larval feeding on different host plants and resources for genomic research on this economically significant pest of maize and suggests that adaptations of D. virgifera larvae to feeding on an alternate host plant invoke fewer transcriptional changes compared to marginal or poor hosts. (via Semantic Scholar)
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2. Zero Hunger (Web of Science)
Sources: Web Of Science, NC State University Libraries, Crossref
Added: February 13, 2023

2023 journal article

An Optimized Small-Scale Rearing System to Support Embryonic Microinjection Protocols for Western Corn Rootworm, Diabrotica virgifera virgifera

Insects, 14(8), 683.

By: F. Chu n, P. Wu n, S. Pinzi n, N. Grubbs n, A. Cohen n  & M. Lorenzen n 

author keywords: insect rearing; rootworm; molecular biology; function genomics; CRISPR
topics (OpenAlex): Insect Resistance and Genetics; CRISPR and Genetic Engineering; Viral Infectious Diseases and Gene Expression in Insects
TL;DR: An optimized rearing system for use in western corn rootworm functional genomics research is reported, specifically the development of a system that facilitates the collection of preblastoderm embryos for microinjection as well as gathering large larvae and pupae for downstream phenotypic screening. (via Semantic Scholar)
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2. Zero Hunger (Web of Science)
Sources: Web Of Science, NC State University Libraries, ORCID, Crossref
Added: September 11, 2023

2021 journal article

Microinjection of Corn Planthopper, Peregrinus maidis, Embryos for CRISPR/Cas9 Genome Editing

Journal of Visualized Experiments, 3(169).

By: W. Klobasa n, F. Chu n, O. Huot n, N. Grubbs n, D. Rotenberg n , A. Whitfield n , M. Lorenzen n 

MeSH headings : Animals; CRISPR-Cas Systems / genetics; Endonucleases / genetics; Female; Gene Editing / methods; Zea mays / chemistry
topics (OpenAlex): CRISPR and Genetic Engineering; Plant Virus Research Studies; Insect symbiosis and bacterial influences
TL;DR: Detailed protocols for collecting and microinjecting precellular P. Maidis embryos with CRISPR components (Cas9 nuclease that has been complexed with guide RNAs), and results of Cas9-based gene knockout of a P. maidis eye-color gene, white, are presented. (via Semantic Scholar)
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Sources: Web Of Science, NC State University Libraries, Crossref
Added: May 17, 2021

2020 journal article

New Technologies for Studying Negative-Strand RNA Viruses in Plant and Arthropod Hosts

Molecular Plant-Microbe Interactions®, 33(3), 382–393.

By: T. German*, M. Lorenzen n , N. Grubbs n & A. Whitfield n 

author keywords: virus-plant interactions
MeSH headings : Animals; Insect Vectors / virology; Insecta / virology; Plant Diseases / virology; Plant Viruses; Plants / virology; RNA Viruses
topics (OpenAlex): Plant Virus Research Studies; Insect-Plant Interactions and Control; Insect symbiosis and bacterial influences
TL;DR: Functional analysis of genes using genome editing will provide future pathways for further study of the transmission cycle and new control strategies for these viruses and their vectors. (via Semantic Scholar)
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2. Zero Hunger (Web of Science)
13. Climate Action (Web of Science)
Sources: Web Of Science, NC State University Libraries, Crossref, ORCID
Added: August 3, 2020

2019 journal article

Structural and functional insights into the Diabrotica virgifera virgifera ATP-binding cassette transporter gene family

BMC Genomics, 20(1).

By: F. Adedipe n, N. Grubbs n, B. Coates *, B. Wiegmman n  & M. Lorenzen n 

author keywords: ATP-binding cassette (ABC) transporter; Phylogenetic; Transcriptome; RNA interference (RNAi); Corn rootworm
MeSH headings : ATP-Binding Cassette Transporters / genetics; Animals; Coleoptera / classification; Coleoptera / genetics; Coleoptera / growth & development; Computational Biology / methods; Gene Expression Profiling; Gene Ontology; Life Cycle Stages / genetics; Molecular Sequence Annotation; Multigene Family; Phenotype; RNA Interference; Transcriptome
topics (OpenAlex): Insect Resistance and Genetics; Advanced biosensing and bioanalysis techniques; Entomopathogenic Microorganisms in Pest Control
TL;DR: This study has produced a relatively comprehensive de novo transcriptome assembly for D. v. virgifera and identified 65 members of the ABC transporter family and provided the first insights into the developmental and physiological roles of ABC transporters in this pest species. (via Semantic Scholar)
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2. Zero Hunger (Web of Science)
Sources: Web Of Science, NC State University Libraries, Crossref
Added: December 16, 2019

2018 journal article

Genome of the small hive beetle (Aethina tumida, Coleoptera: Nitidulidae), a worldwide parasite of social bee colonies, provides insights into detoxification and herbivory

GigaScience, 7(12).

By: J. Evans *, D. McKenna *, E. Scully *, S. Cook *, B. Dainat *, N. Egekwu*, N. Grubbs n, D. Lopez* ...

author keywords: Coleoptera; pollination; Apis mellifera; invasive pest; phytophagy; glycoside hydrolase; honey bee
MeSH headings : ATP-Binding Cassette Transporters / classification; ATP-Binding Cassette Transporters / genetics; ATP-Binding Cassette Transporters / metabolism; Acetylcholinesterase / classification; Acetylcholinesterase / genetics; Acetylcholinesterase / metabolism; Animals; Bees / parasitology; Coleoptera / classification; Coleoptera / genetics; Genetic Variation; Genome; Glycoside Hydrolases / classification; Glycoside Hydrolases / genetics; Glycoside Hydrolases / metabolism; Herbivory; Insect Proteins / classification; Insect Proteins / genetics; Insect Proteins / metabolism; Insecticides / metabolism; Phylogeny; Receptors, Cell Surface / classification; Receptors, Cell Surface / genetics; Receptors, Cell Surface / metabolism; Voltage-Gated Sodium Channels / classification; Voltage-Gated Sodium Channels / genetics
topics (OpenAlex): Insect and Pesticide Research; Insect and Arachnid Ecology and Behavior; Insect-Plant Interactions and Control
TL;DR: The ATUMI genome is sequenced and annotated, providing the first genomic resources for this species and for the Nitidulidae, a beetle family that is closely related to the extraordinarily species-rich clade of beetles known as the Phytophaga. (via Semantic Scholar)
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15. Life on Land (Web of Science)
Sources: Web Of Science, NC State University Libraries, Crossref
Added: February 11, 2019

2018 journal article

Microinjection of Western Corn Rootworm, Diabrotica virgifera virgifera, Embryos for Germline Transformation, or CRISPR/Cas9 Genome Editing

Journal of Visualized Experiments, (134).

By: F. Chu n, P. Wu n, S. Pinzi n, N. Grubbs n & M. Lorenzen n 

author keywords: Bioengineering; Issue 134; embryonic microinjection; functional genomics; germline transformation; transgenesis; CRISPR/Cas9 genome editing; western corn rootworm
MeSH headings : Animals; CRISPR-Cas Systems / genetics; Coleoptera; Gene Editing / methods; Germ-Line Mutation / genetics; Microinjections / methods; RNA Interference
topics (OpenAlex): Insect Resistance and Genetics; CRISPR and Genetic Engineering; Viral Infectious Diseases and Gene Expression in Insects
TL;DR: The methods used to collect and microinject precellular WCR embryos with nucleic acids are detailed, aimed at the creation of transgenic WCR, and CRISPR/Cas9-genome editing could also be performed using the same protocols, with the only difference being the composition of the solution injected into the embryos. (via Semantic Scholar)
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Sources: Web Of Science, NC State University Libraries, Crossref
Added: October 16, 2018

2017 journal article

Agricultural production: assessment of the potential use of Cas9-mediated gene drive systems for agricultural pest control

Journal of Responsible Innovation, 5(sup1), S98–S120.

By: M. Scott n , F. Gould n , M. Lorenzen n , N. Grubbs n, O. Edwards * & D. O’Brochta*

author keywords: Gene drive; Cas9; sterile insect technique; agricultural pest insect
topics (OpenAlex): CRISPR and Genetic Engineering; Insect Resistance and Genetics; Insect behavior and control techniques
TL;DR: To highlight how gene drives could be useful for control of agricultural insect pests, species that are pests of animals, plants, and animals are selected. (via Semantic Scholar)
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2. Zero Hunger (OpenAlex)
Sources: Web Of Science, NC State University Libraries, Crossref, ORCID
Added: August 6, 2018

2017 journal article

Development and use of a piggyBac -based jumpstarter system in Drosophila suzukii

Archives of Insect Biochemistry and Physiology, 97(3), e21439.

By: F. Chu n, W. Klobasa n, N. Grubbs n & M. Lorenzen n 

author keywords: germline transformation; Minos; mutagenesis; piggyBac; transgenesis
MeSH headings : Animals; Animals, Genetically Modified; DNA Transposable Elements; Drosophila / genetics; Genetic Engineering / methods; Mutagenesis; Transposases
topics (OpenAlex): Insect behavior and control techniques; Insect Resistance and Genetics; CRISPR and Genetic Engineering
TL;DR: It is demonstrated that there is a dramatic increase in transformation rates when germline transformation is performed in a transposase-expressing background, and it is shown that these jumpstarter strains could be used in conjunction with a piggyBac-based donor strain for genome-wide mutagenesis of D. suzukii. (via Semantic Scholar)
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Sources: Web Of Science, NC State University Libraries, Crossref
Added: August 6, 2018

2017 journal article

Germline transformation of the western corn rootworm, Diabrotica virgifera virgifera

Insect Molecular Biology, 26(4), 440–452.

By: F. Chu n, W. Klobasa n, P. Wu n, S. Pinzi n, N. Grubbs n, S. Gorski  n, Y. Cardoza n, M. Lorenzen n 

author keywords: Minos; piggyBac; small-scale rearing; embryonic microinjection
MeSH headings : Animals; Coleoptera / genetics; Female; Gene Transfer Techniques; Luminescent Proteins; Male; Transposases
topics (OpenAlex): Insect Resistance and Genetics; CRISPR and Genetic Engineering; Animal Genetics and Reproduction
TL;DR: Establishing transgenic technologies for this beetle is the first step towards bringing a wide range of transformation‐based tools to bear on understanding WCR biology. (via Semantic Scholar)
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Sources: Web Of Science, NC State University Libraries, Crossref
Added: August 6, 2018

2015 article

The ABCs of Eye Color inTribolium castaneum: Orthologs of theDrosophila white,scarlet, andbrownGenes

Grubbs, N., Haas, S., Beeman, R. W., & Lorenzen, M. D. (2015, January 2). Genetics, Vol. 199, p. 749-.

By: N. Grubbs n, S. Haas*, R. Beeman* & M. Lorenzen n 

author keywords: ABC transporters; eye pigmentation; evolution of development
MeSH headings : ATP-Binding Cassette Transporters / genetics; Animals; Base Sequence; Drosophila Proteins / genetics; Evolution, Molecular; Eye Color / genetics; Eye Proteins / genetics; Genes, Insect; Molecular Sequence Data; Mutation; Tribolium / genetics
topics (OpenAlex): melanin and skin pigmentation; Invertebrate Immune Response Mechanisms; Insect Resistance and Genetics
TL;DR: RNA inhibition of Tcw and Tcst recapitulates the mutant phenotypes, confirming the roles of these genes in normal eye pigmentation, while RNA interference of Tcbw provides further evidence that it has no role in eye pigmented in Tribolium. (via Semantic Scholar)
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Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2013 article

New Components of Drosophila Leg Development Identified through Genome Wide Association Studies

Grubbs, N., Leach, M., Su, X., Petrisko, T., Rosario, J. B., & Mahaffey, J. W. (2013, April 1). PLoS ONE.

By: N. Grubbs n, M. Leach n, X. Su *, T. Petrisko *, J. Rosario n & J. Mahaffey n

MeSH headings : Animals; Cell Communication; Drosophila Proteins / antagonists & inhibitors; Drosophila Proteins / genetics; Drosophila Proteins / metabolism; Drosophila melanogaster / embryology; Drosophila melanogaster / genetics; Drosophila melanogaster / metabolism; Embryo, Nonmammalian; Extremities / embryology; Gene Expression Profiling; Gene Expression Regulation, Developmental; Gene Regulatory Networks; Genome-Wide Association Study; Imaginal Discs / embryology; Imaginal Discs / metabolism; Larva / genetics; Larva / growth & development; Larva / metabolism; Morphogenesis / genetics; Mutation; RNA, Small Interfering / genetics; Signal Transduction
topics (OpenAlex): Developmental Biology and Gene Regulation; Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities; Genetic diversity and population structure
TL;DR: The utility of the Genome Wide Association Study utilizing the natural genetic variation in leg proportionality found in the Drosophila Genetic Reference Panel fly lines is demonstrated, integrating the tools of quantitative and molecular genetics to study developmental processes, and new insights are provided into the pathways and networks involved in Drosophile leg development. (via Semantic Scholar)
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Source: Web Of Science
Added: August 6, 2018

2013 article

The evolving role of the orphan nuclear receptor ftz‐f1, a pair‐rule segmentation gene

Heffer, A., Grubbs, N., Mahaffey, J., & Pick, L. (2013, November 1). Evolution & Development.

By: A. Heffer *, N. Grubbs n, J. Mahaffey n & L. Pick *

MeSH headings : Animals; Drosophila melanogaster / metabolism; Evolution, Molecular; Gene Expression Regulation, Developmental; Insect Proteins / chemistry; Insect Proteins / genetics; Insect Proteins / metabolism; Orphan Nuclear Receptors / chemistry; Orphan Nuclear Receptors / genetics; Orphan Nuclear Receptors / metabolism; Tribolium / embryology; Tribolium / genetics; Tribolium / metabolism
topics (OpenAlex): Developmental Biology and Gene Regulation; RNA Research and Splicing; Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
TL;DR: It is proposed that the dependence of Dm‐Ftz‐F1 on interaction with the homeodomain protein Ftz which is expressed in stripes in Drosophila, loosened constraints on DM‐ftz‐f1 expression, allowing for ubiquitous expression of this pair‐rule gene in Dosophila. (via Semantic Scholar)
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Source: Web Of Science
Added: August 6, 2018

2007 article

The appendage role of insect disco genes and possible implications on the evolution of the maggot larval form

Patel, M., Farzana, L., Robertson, L. K., Hutchinson, J., Grubbs, N., Shepherd, M. N., & Mahaffey, J. W. (2007, June 28). Developmental Biology.

By: M. Patel n, L. Farzana n, L. Robertson n, J. Hutchinson n, N. Grubbs n, M. Shepherd n, J. Mahaffey n

author keywords: drosophila; segment identity; zinc finger; appendage; limb; pattern formation
MeSH headings : Animals; Biological Evolution; Body Patterning; DNA-Binding Proteins / metabolism; Drosophila / embryology; Drosophila / growth & development; Drosophila / metabolism; Drosophila Proteins / metabolism; Head / embryology; Head / growth & development; Larva / growth & development; Larva / metabolism; Transcription Factors / metabolism; Tribolium / embryology; Tribolium / growth & development; Tribolium / metabolism
topics (OpenAlex): Developmental Biology and Gene Regulation; Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
TL;DR: It is proposed that the disco genes always function as appendage factors, but their appendage nature is masked during Drosophila embryogenesis due to the reduction of limb fields in the maggot style Drosophile larva. (via Semantic Scholar)
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Source: Web Of Science
Added: August 6, 2018

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