Works (14)
Updated: September 12th, 2023 05:02
2023 journal article
A draft Diabrotica virgifera virgifera genome: insights into control and host plant adaption by a major maize pest insect
BMC Genomics, 24(1).
author keywords: Genome assembly; Host plant specialization; Differential expression
MeSH headings : Animals; Zea mays / physiology; Coleoptera / genetics; Larva / metabolism; Poaceae / genetics; Insecticides / metabolism; Pest Control, Biological; Plants, Genetically Modified / genetics; Endotoxins
TL;DR:
Insight is provided into transcriptomic responses of larval feeding on different host plants and resources for genomic research on this economically significant pest of maize and suggests that adaptations of D. virgifera larvae to feeding on an alternate host plant invoke fewer transcriptional changes compared to marginal or poor hosts.
(via Semantic Scholar)
open access via bmcgenomics.biomedcentral.com (publisher PDF)
UN Sustainable Development Goal Categories
2. Zero Hunger
(Web of Science)
Sources: Web Of Science, NC State University Libraries, Crossref
Added: February 13, 2023
2023 journal article
An Optimized Small-Scale Rearing System to Support Embryonic Microinjection Protocols for Western Corn Rootworm, Diabrotica virgifera virgifera
Insects, 14(8), 683.
author keywords: insect rearing; rootworm; molecular biology; function genomics; CRISPR
TL;DR:
An optimized rearing system for use in western corn rootworm functional genomics research is reported, specifically the development of a system that facilitates the collection of preblastoderm embryos for microinjection as well as gathering large larvae and pupae for downstream phenotypic screening.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger
(Web of Science)
Sources: Web Of Science, NC State University Libraries, ORCID, Crossref
Added: September 11, 2023
2021 journal article
Microinjection of Corn Planthopper, <em>Peregrinus maidis</em>, Embryos for CRISPR/Cas9 Genome Editing
Journal of Visualized Experiments, 3(169).
MeSH headings : Animals; CRISPR-Cas Systems / genetics; Endonucleases / genetics; Female; Gene Editing / methods; Zea mays / chemistry
TL;DR:
Detailed protocols for collecting and microinjecting precellular P. Maidis embryos with CRISPR components (Cas9 nuclease that has been complexed with guide RNAs), and results of Cas9-based gene knockout of a P. maidis eye-color gene, white, are presented.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, NC State University Libraries, Crossref
Added: May 17, 2021
2020 journal article
New Technologies for Studying Negative-Strand RNA Viruses in Plant and Arthropod Hosts
Molecular Plant-Microbe Interactions®, 33(3), 382–393.
author keywords: virus-plant interactions
MeSH headings : Animals; Insect Vectors / virology; Insecta / virology; Plant Diseases / virology; Plant Viruses; Plants / virology; RNA Viruses
TL;DR:
Functional analysis of genes using genome editing will provide future pathways for further study of the transmission cycle and new control strategies for these viruses and their vectors.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger
(Web of Science)
13. Climate Action
(Web of Science)
Sources: Web Of Science, NC State University Libraries, Crossref
Added: August 3, 2020
2019 journal article
Structural and functional insights into the Diabrotica virgifera virgifera ATP-binding cassette transporter gene family
BMC Genomics, 20(1).
author keywords: ATP-binding cassette (ABC) transporter; Phylogenetic; Transcriptome; RNA interference (RNAi); Corn rootworm
MeSH headings : ATP-Binding Cassette Transporters / genetics; Animals; Coleoptera / classification; Coleoptera / genetics; Coleoptera / growth & development; Computational Biology / methods; Gene Expression Profiling; Gene Ontology; Life Cycle Stages / genetics; Molecular Sequence Annotation; Multigene Family; Phenotype; RNA Interference; Transcriptome
TL;DR:
This study has produced a relatively comprehensive de novo transcriptome assembly for D. v. virgifera and identified 65 members of the ABC transporter family and provided the first insights into the developmental and physiological roles of ABC transporters in this pest species.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger
(Web of Science)
Sources: Web Of Science, NC State University Libraries, Crossref
Added: December 16, 2019
2018 journal article
Genome of the small hive beetle (Aethina tumida, Coleoptera: Nitidulidae), a worldwide parasite of social bee colonies, provides insights into detoxification and herbivory
GigaScience, 7(12).
author keywords: Coleoptera; pollination; Apis mellifera; invasive pest; phytophagy; glycoside hydrolase; honey bee
MeSH headings : ATP-Binding Cassette Transporters / classification; ATP-Binding Cassette Transporters / genetics; ATP-Binding Cassette Transporters / metabolism; Acetylcholinesterase / classification; Acetylcholinesterase / genetics; Acetylcholinesterase / metabolism; Animals; Bees / parasitology; Coleoptera / classification; Coleoptera / genetics; Genetic Variation; Genome; Glycoside Hydrolases / classification; Glycoside Hydrolases / genetics; Glycoside Hydrolases / metabolism; Herbivory; Insect Proteins / classification; Insect Proteins / genetics; Insect Proteins / metabolism; Insecticides / metabolism; Phylogeny; Receptors, Cell Surface / classification; Receptors, Cell Surface / genetics; Receptors, Cell Surface / metabolism; Voltage-Gated Sodium Channels / classification; Voltage-Gated Sodium Channels / genetics
TL;DR:
The ATUMI genome is sequenced and annotated, providing the first genomic resources for this species and for the Nitidulidae, a beetle family that is closely related to the extraordinarily species-rich clade of beetles known as the Phytophaga.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
15. Life on Land
(Web of Science)
Sources: Web Of Science, NC State University Libraries, Crossref
Added: February 11, 2019
2018 journal article
Microinjection of Western Corn Rootworm, <em>Diabrotica virgifera virgifera</em>, Embryos for Germline Transformation, or CRISPR/Cas9 Genome Editing
Journal of Visualized Experiments, (134).
author keywords: Bioengineering; Issue 134; embryonic microinjection; functional genomics; germline transformation; transgenesis; CRISPR/Cas9 genome editing; western corn rootworm
MeSH headings : Animals; CRISPR-Cas Systems / genetics; Coleoptera; Gene Editing / methods; Germ-Line Mutation / genetics; Microinjections / methods; RNA Interference
TL;DR:
The methods used to collect and microinject precellular WCR embryos with nucleic acids are detailed, aimed at the creation of transgenic WCR, and CRISPR/Cas9-genome editing could also be performed using the same protocols, with the only difference being the composition of the solution injected into the embryos.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, NC State University Libraries, Crossref
Added: October 16, 2018
2017 journal article
Agricultural production: assessment of the potential use of Cas9-mediated gene drive systems for agricultural pest control
Journal of Responsible Innovation, 5(sup1), S98–S120.
author keywords: Gene drive; Cas9; sterile insect technique; agricultural pest insect
TL;DR:
To highlight how gene drives could be useful for control of agricultural insect pests, species that are pests of animals, plants, and animals are selected.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger
(OpenAlex)
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, NC State University Libraries, Crossref, ORCID
Added: August 6, 2018
2017 journal article
Development and use of a piggyBac -based jumpstarter system in Drosophila suzukii
Archives of Insect Biochemistry and Physiology, 97(3), e21439.
author keywords: germline transformation; Minos; mutagenesis; piggyBac; transgenesis
MeSH headings : Animals; Animals, Genetically Modified; DNA Transposable Elements; Drosophila / genetics; Genetic Engineering / methods; Mutagenesis; Transposases
TL;DR:
It is demonstrated that there is a dramatic increase in transformation rates when germline transformation is performed in a transposase-expressing background, and it is shown that these jumpstarter strains could be used in conjunction with a piggyBac-based donor strain for genome-wide mutagenesis of D. suzukii.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, NC State University Libraries, Crossref
Added: August 6, 2018
2017 journal article
Germline transformation of the western corn rootworm, Diabrotica virgifera virgifera
Insect Molecular Biology, 26(4), 440–452.
author keywords: Minos; piggyBac; small-scale rearing; embryonic microinjection
MeSH headings : Animals; Coleoptera / genetics; Female; Gene Transfer Techniques; Luminescent Proteins; Male; Transposases
TL;DR:
Establishing transgenic technologies for this beetle is the first step towards bringing a wide range of transformation‐based tools to bear on understanding WCR biology.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, NC State University Libraries, Crossref
Added: August 6, 2018
2015 journal article
The ABCs of Eye Color in Tribolium castaneum: Orthologs of the Drosophila white, scarlet, and brown Genes
GENETICS, 199(3), 749-+.
author keywords: ABC transporters; eye pigmentation; evolution of development
MeSH headings : ATP-Binding Cassette Transporters / genetics; Animals; Base Sequence; Drosophila Proteins / genetics; Evolution, Molecular; Eye Color / genetics; Eye Proteins / genetics; Genes, Insect; Molecular Sequence Data; Mutation; Tribolium / genetics
TL;DR:
RNA inhibition of Tcw and Tcst recapitulates the mutant phenotypes, confirming the roles of these genes in normal eye pigmentation, while RNA interference of Tcbw provides further evidence that it has no role in eye pigmented in Tribolium.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018
2013 journal article
New Components of Drosophila Leg Development Identified through Genome Wide Association Studies
PLOS ONE, 8(4).
MeSH headings : Animals; Cell Communication; Drosophila Proteins / antagonists & inhibitors; Drosophila Proteins / genetics; Drosophila Proteins / metabolism; Drosophila melanogaster / embryology; Drosophila melanogaster / genetics; Drosophila melanogaster / metabolism; Embryo, Nonmammalian; Extremities / embryology; Gene Expression Profiling; Gene Expression Regulation, Developmental; Gene Regulatory Networks; Genome-Wide Association Study; Imaginal Discs / embryology; Imaginal Discs / metabolism; Larva / genetics; Larva / growth & development; Larva / metabolism; Morphogenesis / genetics; Mutation; RNA, Small Interfering / genetics; Signal Transduction
TL;DR:
The utility of the Genome Wide Association Study utilizing the natural genetic variation in leg proportionality found in the Drosophila Genetic Reference Panel fly lines is demonstrated, integrating the tools of quantitative and molecular genetics to study developmental processes, and new insights are provided into the pathways and networks involved in Drosophile leg development.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: Web Of Science
Added: August 6, 2018
2013 review
The evolving role of the orphan nuclear receptor ftz-f1, a pair-rule segmentation gene
[Review of ]. EVOLUTION & DEVELOPMENT, 15(6), 406–417.
MeSH headings : Animals; Drosophila melanogaster / metabolism; Evolution, Molecular; Gene Expression Regulation, Developmental; Insect Proteins / chemistry; Insect Proteins / genetics; Insect Proteins / metabolism; Orphan Nuclear Receptors / chemistry; Orphan Nuclear Receptors / genetics; Orphan Nuclear Receptors / metabolism; Tribolium / embryology; Tribolium / genetics; Tribolium / metabolism
TL;DR:
It is proposed that the dependence of Dm‐Ftz‐F1 on interaction with the homeodomain protein Ftz which is expressed in stripes in Drosophila, loosened constraints on DM‐ftz‐f1 expression, allowing for ubiquitous expression of this pair‐rule gene in Dosophila.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: Web Of Science
Added: August 6, 2018
2007 journal article
The appendage role of insect disco genes and possible implications on the evolution of the maggot larval form
DEVELOPMENTAL BIOLOGY, 309(1), 56–69.
author keywords: drosophila; segment identity; zinc finger; appendage; limb; pattern formation
MeSH headings : Animals; Biological Evolution; Body Patterning; DNA-Binding Proteins / metabolism; Drosophila / embryology; Drosophila / growth & development; Drosophila / metabolism; Drosophila Proteins / metabolism; Head / embryology; Head / growth & development; Larva / growth & development; Larva / metabolism; Transcription Factors / metabolism; Tribolium / embryology; Tribolium / growth & development; Tribolium / metabolism
TL;DR:
It is proposed that the disco genes always function as appendage factors, but their appendage nature is masked during Drosophila embryogenesis due to the reduction of limb fields in the maggot style Drosophile larva.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: Web Of Science
Added: August 6, 2018
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