Orlando Arguello-Miranda
Also known as: Alejandrolvido (GitHub)
Cell Biology; Machine Learning; Spectral microscopy
Orlando Argüello-Miranda grew up in Costa Rica. After receiving a B.Sc. from the National University, he moved to Germany to pursue a PhD in cell biology at the Max Planck Institute for Cell Biology and Genetics in Dresden. He completed his doctoral dissertation working at the Max Planck Institute for Biochemistry in Munich. His thesis described the regulatory protein network that controls major transitions during specialized forms of cell division, such as meiosis. Orlando joined the laboratory of the late Andreas Doncic at UT Southwestern in Texas for his postdoctoral work, where he earned a K99 Pathway to Independence Award from the National Institutes of Health (NIH-GMS) under the supervision of Gaudenz Danuser. In Augusts 2022, Orlando officially started his own laboratory at the department of Plant and Microbial Biology at North Carolina State University. His group's research aims to understand how cells control cell division during dormant or quiescent states. Orlando likes to spend time writing fiction and studying languages.
Works (15)
Updated: November 5th, 2024 19:25
2024 article
Deep learning-driven imaging of cell division and cell growth across an entire eukaryotic life cycle
Ramakanth, S., Kennedy, T., Yalcinkaya, B., Neupane, S., Tadic, N., Buchler, N. E., & Argüello-Miranda, O. (2024, April 27).
open access via doi.org (repository)
Source: ORCID
Added: May 1, 2024
2024 article
Multi-signal regulation of the GSK-3β homolog Rim11 controls meiosis entry in budding yeast
Kociemba, J., Jorgensen, A. C. S., Tadic, N., Harris, A., Sideri, T., Chan, W. Y., … Werven, F. J. (2024, June 17). EMBO JOURNAL, Vol. 6.
author keywords: Rim11; GSK-3 beta; Meiosis; Yeast; Ume6
Sources: Web Of Science, NC State University Libraries
Added: July 1, 2024
2023 article
Multi-signal regulation of the GSK-3β homolog Rim11 governs meiosis entry in yeast
Kociemba, J., Jørgensen, A. C. S., Tadic, N., Harris, A., Sideri, T., Chan, W. Y., … Werven, F. (2023, September 22).
TL;DR:
It is proposed that the signalling-regulatory network described here generates robustness in cell-fate control in meiosis by integrating multiple input signals to control Ume6 phosphorylation and EMG transcription.
(via Semantic Scholar)
Source: ORCID
Added: April 1, 2024
2022 article
Cdc14 plans autophagy for meiotic cell divisions
Feng, W., Arguello-Miranda, O., Qian, S., & Wang, F. (2022, May 28). AUTOPHAGY, Vol. 18.
author keywords: Atg1; Atg13; autophagy; Cdc14; meiosis; phosphatase; sporulation
MeSH headings : Adaptor Proteins, Signal Transducing / metabolism; Autophagy; Autophagy-Related Proteins / metabolism; Meiosis; Saccharomyces cerevisiae Proteins / metabolism
TL;DR:
In vitro and in vivo assays revealed that the conserved phosphatase Cdc14 stimulates autophagy initiation during meiotic divisions, specifically in anaphase I and II, when a subpopulation of active CDC14 relocates to the cytosol and interacts with phagophore assembly sites (PAS) triggering the dephosphorylation of Atg13 to stimulate Atg1 kinase activity and autophagic activity.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, ORCID, NC State University Libraries
Added: June 6, 2022
2022 journal article
Cdc14 spatiotemporally dephosphorylates Atg13 to activate autophagy during meiotic divisions
JOURNAL OF CELL BIOLOGY, 221(5).
MeSH headings : Adaptor Proteins, Signal Transducing / genetics; Adaptor Proteins, Signal Transducing / metabolism; Anaphase; Autophagy; Autophagy-Related Proteins / genetics; Autophagy-Related Proteins / metabolism; Cell Cycle Proteins / genetics; Cell Cycle Proteins / metabolism; Meiosis; Protein Tyrosine Phosphatases / genetics; Protein Tyrosine Phosphatases / metabolism; Saccharomyces cerevisiae Proteins / genetics; Saccharomyces cerevisiae Proteins / metabolism
TL;DR:
A meiosis-tailored mechanism of Cdc14 that governs autophagy is reported that stimulates Atg1 kinase activity and thus Autophagy at anaphases I and anaphase II to facilitate meiosis progression, meiosis exit, and sporulation.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: ORCID, Web Of Science, NC State University Libraries
Added: July 11, 2022
2022 journal article
Live-cell fluorescence spectral imaging as a data science challenge
Biophysical Reviews.
TL;DR:
This review aims to provide both the experimental scientist and the data analyst with a straightforward description of the evolution of spectral unmixing algorithms for fluorescence live-cell imaging, and shows how the initial systems of linear equations used to determine the concentration of fluorophores in a pixel progressively evolved into matrix factorization, clustering, and deep learning approaches.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: ORCID
Added: July 11, 2022
2021 journal article
Cell cycle-independent integration of stress signals by Xbp1 promotes Non-G1/G0 quiescence entry
JOURNAL OF CELL BIOLOGY, 221(1).
MeSH headings : Cell Cycle; Cell Nucleus / metabolism; Cell Proliferation; Microfluidics; Repressor Proteins / metabolism; Saccharomyces cerevisiae / cytology; Saccharomyces cerevisiae / metabolism; Saccharomyces cerevisiae Proteins / metabolism; Signal Transduction; Stress, Physiological; Transcription Factors / metabolism
TL;DR:
This work uses machine learning and spectral imaging to show how the stress-activated transcriptional repressor Xbp1 can drive cellular quiescence—a reversible arrest of proliferation crucial for stress survival and development—even outside the G1 cell cycle stage.
(via Semantic Scholar)
Sources: ORCID, Web Of Science
Added: November 8, 2021
2021 article
Cell cycle-independent integration of stress signals promotes Non-G1/G0 quiescence entry
Argüello-Miranda, O., Marchand, A., Kennedy, T., Russo, M. A. X., & Noh, J. (2021, March 15).
Source: ORCID
Added: August 8, 2024
2020 journal article
Functional interrelationships between carbohydrate and lipid storage, and mitochondrial activity during sporulation in Saccharomyces cerevisiae
Yeast.
Contributors: Y. Liu *, N. Wood *, A. Marchand *, * & A. Doncic*
author keywords: cyclin 3; glycogen; lipid droplet; meiosis; trehalose; yeast
MeSH headings : Carbohydrates / chemistry; Cyclins / genetics; Cyclins / metabolism; DNA Replication; Gene Expression Regulation, Fungal; Lipids / chemistry; Meiosis; Mitochondria / metabolism; Saccharomyces cerevisiae / metabolism; Saccharomyces cerevisiae Proteins / genetics; Saccharomyces cerevisiae Proteins / metabolism; Spores, Fungal / physiology; Transcriptome
TL;DR:
Results provide new insights into the complex crosstalk between metabolic factors that support gametogenesis and storage carbohydrate‐deficient strains exhibited a greater dependency on mitochondrial activity and lipid stores than wild‐type yeast.
(via Semantic Scholar)
Source: ORCID
Added: July 11, 2022
2019 journal article
Meiosis research in orphan and non-orphan tropical crops
Frontiers in Plant Science, 10, 1–7.
Contributors: P. Bolaños-Villegas * & *
author keywords: meiosis; plant breeding; genetic diversity; tropical agriculture; food security; climate change
TL;DR:
This review covers technical approaches to engineer key meiotic genes in tropical crops as a blueprint for future work and examples of tropical crops in which such strategies could be applied.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger
(OpenAlex)
Source: ORCID
Added: July 11, 2022
2018 journal article
Integration of Multiple Metabolic Signals Determines Cell Fate Prior to Commitment
Molecular Cell, 71(5), 733–744.e11.
MeSH headings : Bayes Theorem; Meiosis / physiology; Metabolic Networks and Pathways / physiology; Saccharomycetales / metabolism; Saccharomycetales / physiology
TL;DR:
The results show that cells can decide their future fates long before commitment mechanisms are activated, when multiple metabolic parameters simultaneously cross cell-fate-specific thresholds.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
16. Peace, Justice and Strong Institutions
(OpenAlex)
Source: ORCID
Added: July 11, 2022
2017 journal article
Towards a Unified Understanding of Lithium Action in Basic Biology and its Significance for Applied Biology
Journal of Membrane Biology, 250(6), 587–604.
Contributors: E. Jakobsson *, * , S. Chiu*, Z. Fazal *, J. Kruczek*, S. Nunez-Corrales *, S. Pandit *, L. Pritchet
author keywords: Ion channels and transporters; Magnesium-dependent enzymes; Physical properties of biological membranes
MeSH headings : Enzymes / metabolism; Ion Channels / metabolism; Lithium / metabolism; Magnesium / metabolism
TL;DR:
This review will consider what is known about the nature of lithium exerts its myriad physiological and biochemical effects by competing for macromolecular sites that are relatively specific for other cations and suggest using and extending this knowledge towards the goal of a unified understanding of lithium in biology and the application of that understanding in medicine and nutrition.
(via Semantic Scholar)
Source: ORCID
Added: July 11, 2022
2016 journal article
Casein Kinase 1 Coordinates Cohesin Cleavage, Gametogenesis, and Exit from M Phase in Meiosis II
Developmental Cell, 40(1), 37–52.
Contributors: * , I. Zagoriy *, V. Mengoli*, J. Rojas *, K. Jonak *, T. Oz *, P. Graf*, W. Zachariae *
MeSH headings : Anaphase; Casein Kinase I / metabolism; Cell Cycle Proteins / metabolism; Cell Nucleus / metabolism; Centromere / metabolism; Chromosomal Proteins, Non-Histone / metabolism; Gametogenesis; Meiosis; Phosphorylation; Protein Phosphatase 2 / metabolism; Proteolysis; Saccharomyces cerevisiae / cytology; Saccharomyces cerevisiae / metabolism; Saccharomyces cerevisiae Proteins / metabolism; Separase / metabolism; Spindle Apparatus / metabolism
TL;DR:
Hrr25 synchronizes formation of the single-copy genome with gamete differentiation and termination of meiosis, and mediates exit from meiosis II by activating pathways that trigger the destruction of M-phase-promoting kinases.
(via Semantic Scholar)
Source: ORCID
Added: July 11, 2022
2012 journal article
Meiotic prophase requires proteolysis of m phase regulators mediated by the meiosis-specific APC/CAma1
Cell, 151(3), 603–618.
Contributors: E. Okaz*, * , A. Bogdanova*, P. Vinod *, J. Lipp*, Z. Markova*, I. Zagoriy *, B. Novak *, W. Zachariae *
MeSH headings : Anaphase-Promoting Complex-Cyclosome; Cdc20 Proteins; Cell Cycle Proteins / metabolism; Chromosome Segregation; Chromosomes, Fungal / metabolism; DNA-Binding Proteins / metabolism; Meiosis; Metaphase; Prophase; Protein Serine-Threonine Kinases / metabolism; Proteolysis; Saccharomyces cerevisiae / cytology; Saccharomyces cerevisiae / metabolism; Saccharomyces cerevisiae Proteins / metabolism; Spindle Apparatus; Transcription Factors / metabolism; Ubiquitin-Protein Ligase Complexes / metabolism
TL;DR:
It is shown that an extended prophase I additionally requires the suppression of latent, mitotic cell-cycle controls by the anaphase-promoting complex (APC/C) and its meiosis-specific activator Ama1, which trigger the degradation of M phase regulators and Ndd1, a subunit of a mitotic transcription factor.
(via Semantic Scholar)
Source: ORCID
Added: July 11, 2022
2008 journal article
Interchromatidal central ridge and transversal symmetry in early metaphasic human chromosome one
Journal of Molecular Recognition, 21(3), 184–189.
Contributors: * & G. Sáenz-Arce *
MeSH headings : Adult; Chromatids / metabolism; Chromatids / ultrastructure; Chromosome Banding; Chromosomes, Human, Pair 1 / metabolism; Chromosomes, Human, Pair 1 / ultrastructure; Euchromatin / ultrastructure; Female; Heterochromatin / ultrastructure; Humans; Male; Metaphase
TL;DR:
It is suggested that this central ridge and symmetry patterns point out a transitional arrangement of the early metaphase chromosome and support evidence for interchromatidal interactions prior to disjunction.
(via Semantic Scholar)
Source: ORCID
Added: July 11, 2022
Employment
Updated: March 15th, 2023 14:48
2022 - present
North Carolina State University Raleigh, North Carolina, US
Assistant Professor
Plant and Microbial Biology
Education
Updated: February 26th, 2020 10:58
2012 - 2015
Max-Planck-Institut für Biochemie Martinsried, Bayern, DE
PHD
Chromosome Biology
Funding History
Funding history based on the linked ORCID record. Updated: October 4th, 2023 16:46
grant
March 1, 2020 - July 31, 2025
Systems biology of quiescence entry
National Institute of General Medical Sciences
grant
March 1, 2020 - February 28, 2022
Systems biology of quiescence entry
National Institute of General Medical Sciences
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