@article{sloniker_raftopoulou_chen_ryser_beaudry_2023, title={Fate of Planktonic and Biofilm-Derived <i>Listeria monocytogenes</i> on Unwaxed Apples during Air and Controlled Atmosphere Storage}, volume={12}, ISSN={["2304-8158"]}, DOI={10.3390/foods12193673}, abstractNote={Multiple recalls and outbreaks involving Listeria monocytogenes-contaminated apples have been linked to the post-harvest packing environment where this pathogen can persist in biofilms. Therefore, this study assessed L. monocytogenes survival on apples as affected by harvest year, apple cultivar, storage atmosphere, and growth conditions. Unwaxed Gala, Granny Smith, and Honeycrisp apples were dip-inoculated in an 8-strain L. monocytogenes cocktail of planktonic- or biofilm-grown cells (~6.5 log CFU/mL), dried, and then examined for numbers of L. monocytogenes during air or controlled atmosphere (CA) (1.5% O2, 1.5% CO2) storage at 2 °C. After 90 days, air or CA storage yielded similar L. monocytogenes survival (p > 0.05), regardless of harvest year. Populations gradually decreased with L. monocytogenes quantifiable in most samples after 7 months. Apple cultivar significantly impacted L. monocytogenes survival (p < 0.05) during both harvest years with greater reductions (p < 0.05) seen on Gala compared to Granny Smith and Honeycrisp. Biofilm-derived cells survived longer (p < 0.05) on L. monocytogenes-inoculated Gala and Honeycrisp apples compared to cells grown planktonically. These findings should aid in the development of improved L. monocytogenes intervention strategies for apple growers and packers.}, number={19}, journal={FOODS}, author={Sloniker, Natasha and Raftopoulou, Ourania and Chen, Yi and Ryser, Elliot T. and Beaudry, Randy}, year={2023}, month={Oct} }
 @article{raftopoulou_barrangou_2023, title={Mining microbial organisms to discover and characterize novel CRISPR-Cas systems}, volume={27}, ISSN={["2468-4511"]}, url={https://doi.org/10.1016/j.cobme.2023.100469}, DOI={10.1016/j.cobme.2023.100469}, abstractNote={The need for new genome manipulation tools is leading the way for the continued discovery of novel clustered regularly interspaced short palindromic repeats— CRISPR associated sequences (CRISPR-Cas) systems. Researchers have been analyzing the genomes of prokaryotes and more recently metagenomic sequencing data to find novel and diverse CRISPR-Cas systems and their associated genome editing effectors. In this review, we provide an overview of in silico, in vitro, and in vivo analyses performed to characterize key elements of CRISPR-Cas systems, encompassing the CRISPR array, Cas proteins, guide ribonucleic acid (RNAs), and protospacer-adjacent motif (PAM) which defines targeting. We also highlight subsequent in vitro and in vivo assays employed to validate CRISPR function and Cas effector activity in the context of genome editing in various cellular contexts.}, journal={CURRENT OPINION IN BIOMEDICAL ENGINEERING}, author={Raftopoulou, Ourania and Barrangou, Rodolphe}, year={2023}, month={Sep} }
 @article{lianou_raftopoulou_spyrelli_nychas_2021, title={Growth of Listeria monocytogenes in Partially Cooked Battered Chicken Nuggets as a Function of Storage Temperature}, volume={10}, ISSN={["2304-8158"]}, DOI={10.3390/foods10030533}, abstractNote={Battered poultry products may be wrongly regarded and treated by consumers as ready-to-eat and, as such, be implicated in foodborne disease outbreaks. This study aimed at the quantitative description of the growth behavior of Listeria monocytogenes in fresh, partially cooked (non-ready-to-eat) battered chicken nuggets as function of temperature. Commercially prepared chicken breast nuggets were inoculated with L. monocytogenes and stored at different isothermal conditions (4, 8, 12, and 16 °C). The pathogen’s growth behavior was characterized via a two-step predictive modelling approach: estimation of growth kinetic parameters using a primary model, and description of the effect of temperature on the estimated maximum specific growth rate (μmax) using a secondary model. Model evaluation was undertaken using independent growth data under both constant and dynamic temperature conditions. According to the findings of this study, L. monocytogenes may proliferate in battered chicken nuggets in the course of their shelf life to levels potentially hazardous for susceptible population groups, even under well-controlled refrigerated storage conditions. Model evaluation demonstrated a satisfactory performance, where the estimated bias factor (Bf) was 0.92 and 1.08 under constant and dynamic temperature conditions, respectively, while the accuracy factor (Af) value was 1.08, in both cases. The collected data should be useful in model development and quantitative microbiological risk assessment in battered poultry products.}, number={3}, journal={FOODS}, author={Lianou, Alexandra and Raftopoulou, Ourania and Spyrelli, Evgenia and Nychas, George-John E.}, year={2021}, month={Mar} }