@article{ruddle_whetten_cardinal_upchurch_miranda_2014, title={Effect of Delta 9-stearoyl-ACP-desaturase-C mutants in a high oleic background on soybean seed oil composition}, volume={127}, ISSN={["1432-2242"]}, DOI={10.1007/s00122-013-2223-5}, abstractNote={Two new sources of elevated seed stearic acid were identified and the feasibility of an elevated stearic acid, high oleic acid germplasm was studied. Soybean [Glycine max (L.) Merr.] oil typically contains 2-4% stearic acid. Oil with at least 20% stearic acid is desirable because of its improved baking properties and health profile. This study identifies two new sources of high stearic acid and evaluates the interaction of high stearic and oleic acid alleles. TCHM08-1087 and TCHM08-755, high stearic acid 'Holladay' mutants, were crossed to FAM94-41-3, a line containing a point mutation in a seed-specific isoform of a Δ9-stearoyl-acyl carrier protein-desaturase (SACPD-C). F2-derived lines were evaluated for fatty acid content in four field environments. Sequencing of SACPDs in TCHM08-1087 and TCHM08-755 revealed distinct deletions of at least one megabase encompassing SACPD-C in both lines. After genotyping, the additive effect for stearic acid was estimated at +1.8% for the SACPD-C point mutation and +4.1% for the SACPD-C deletions. Average stearic acid in lines homozygous for the deletions was 12.2%. A FAM94-41-3-derived line and TCHM08-1087-11, a selection from TCHM08-1087, were crossed to S09-2902-145, a line containing missense mutations in two fatty acid desaturases (FAD2-1A and FAD2-1B). F1 plants were grown in a greenhouse and individual F2 seed were genotyped and phenotyped. No interaction was observed between either FAD2-1A or FAD2-1B and any of the SACPD-C mutant alleles. Seed homozygous mutant for SACPD-C/FAD2-1A/FAD2-1B contained 12.7% stearic acid and 65.5% oleic acid while seed homozygous for the SACPD-C deletion and mutant for FAD2-1A and FAD2-1B averaged 10.4% stearic acid and 75.9% oleic acid.}, number={2}, journal={THEORETICAL AND APPLIED GENETICS}, author={Ruddle, Paul, II and Whetten, Rebecca and Cardinal, Andrea and Upchurch, Robert G. and Miranda, Lilian}, year={2014}, month={Feb}, pages={349–358} }
 @article{ruddle_cardinal_upchurch_arellano_miranda_2013, title={Agronomic Effects of Mutations in Two Soybean Delta 9-Stearoyl-Acyl Carrier Protein-Desaturases}, volume={53}, ISSN={["1435-0653"]}, DOI={10.2135/cropsci2013.02.0120}, abstractNote={ABSTRACT}, number={5}, journal={CROP SCIENCE}, author={Ruddle, Paul, II and Cardinal, Andrea and Upchurch, Robert G. and Arellano, Consuelo and Miranda, Lilian}, year={2013}, pages={1887–1893} }
 @article{ruddle_whetten_cardinal_upchurch_miranda_2013, title={Effect of a novel mutation in a Delta 9-stearoyl-ACP-desaturase on soybean seed oil composition}, volume={126}, ISSN={["1432-2242"]}, DOI={10.1007/s00122-012-1977-5}, abstractNote={Soybean [Glycine max (L.) Merr.] oil typically contains 2-4% stearic acid. Seed oil with 20% stearic acid would be useful for solid fat applications, both for its cooking properties and health benefits. Breeding lines with high stearic acid have been developed, but many suffer from agronomic problems. This study identifies a new source of high stearic acid, determines its relationship with another high stearic locus and presents molecular markers for it is use in breeding. TCJWB03-806-7-19, a 'Holladay' mutant with high stearic acid, was crossed to two FAM94-41-derived lines that contained a point mutation in a seed-specific isoform of a Δ9-stearoyl-acyl carrier protein-desaturase (SACPD-C). Fatty acid analysis was performed over two growing seasons with F(2)-derived lines and transgressive segregation for stearic acid content was observed. Sequencing of SACPD isoforms in TCJWB03-806-7-19 revealed the deletion of an 'A' nucleotide in exon 3 of SACPD-B, which results in a protein whose final 28 amino acids are predicted to differ from Williams 82 SACPD-B. Sorting intolerant from tolerant (SIFT) analysis revealed that this frameshift mutation may affect SACPD-B protein function. Allele-specific genotyping for the SACPD-C point mutation and SACPD-B nucleotide deletion was performed in both populations. Additive effects and R(2) for stearic acid were +3.3 and 0.55 for SACPD-C and +1.9 and 0.19 for SACPD-B. Average stearic acid in lines homozygous for both mutations was 14.6%. This SACPD-B mutation represents a novel high stearic allele.}, number={1}, journal={THEORETICAL AND APPLIED GENETICS}, author={Ruddle, Paul, II and Whetten, Rebecca and Cardinal, Andrea and Upchurch, Robert G. and Miranda, Lilian}, year={2013}, month={Jan}, pages={241–249} }