@article{rosas-anderson_sinclair_rufty_2021, title={Leaf expansion and recovery from soil drying in soybean genotypes}, volume={35}, ISSN={["1542-7536"]}, DOI={10.1080/15427528.2020.1802801}, abstractNote={ABSTRACT Maintenance and recovery of the canopy area when drought stress is relieved may be critical for maintaining high productivity. In this study, experiments were conducted in controlled environments to evaluate leaf expansion and leaf necrosis of five soybean (Glycine max Merr. L.) genotypes undergoing soil-drying, followed by re-watering. Water-deficit stress was imposed by limiting daily watering. When soil water decreased to a severe defined level, full watering resumed for five days. Measurements of leaf expansion and necrosis were taken during water-deficit and recovery periods. Genotypic differences for critical soil-water thresholds at which leaf expansion rates decline were detected. Genotype “Benning” showed the sensitivity of expansion rates to soil-drying while “Geden Shirazu” showed leaf expansion tolerance to soil drying. All genotypes recovered expansion rates within one or two days. During recovery, the recently released cultivar ‘USDA-N8002ʹ had the highest leaf expansion rate among genotypes, compared to its well-watered plants. The high recovery potential of USDA-N8002 was largely attributable to high nighttime expansion recovery. This elite drought-tolerant cultivar, along with the commercial cultivar Benning, experienced the lowest levels of leaf necrosis. While all genotypes exhibited rapid recovery in leaf expansion following drought, variation in the extent of recovery and level of leaf necrosis indicates that these characteristics can be exploited to enhance drought resilience.}, number={1}, journal={JOURNAL OF CROP IMPROVEMENT}, author={Rosas-Anderson, Pablo and Sinclair, Thomas R. and Rufty, Thomas W.}, year={2021}, month={Jan}, pages={96–110} }
 @article{rosas-anderson_sinclair_locke_carter_rufty_2020, title={Leaf gas exchange recovery of soybean from water-deficit stress}, volume={34}, ISSN={1542-7528 1542-7536}, url={http://dx.doi.org/10.1080/15427528.2020.1764429}, DOI={10.1080/15427528.2020.1764429}, abstractNote={ABSTRACT As the risk of drought attributable to climate change increases, the development of high-yielding, drought-adapted cultivars will be critical for minimizing yield losses in crops like soybean (Glycine max (L.) Merr.). In this study, the ability of soybean genotypes to recover transpiration and leaf gas exchange capacity following re-watering from soil drying was investigated. The plants were subjected to controlled water-deficit stress and recovery in growth-chamber experiments. Transpiration was measured on five soybean genotypes and photosynthesis rates on two select genotypes. After water re-supply, transpiration was initially low but increased until a stable rate was reached on day 3, to about 50% to 100% of the rates of reference plants that had not been stressed. The largest difference in maximum transpiration recovery was between the varieties USDA-N8002 and Benning compared to the landrace Geden Shirazu, with Geden Shirazu having the lowest recovery. Photosynthesis and vapor-pressure-deficit response measurements did not show that restricted plant stomatal conductance was responsible for the limitation observed in Geden Shirazu recovery. Since all genotypes showed rapid recovery from water-deficit stress in 3 d, more rapid recovery was not indicated as a major candidate for improving soybean drought tolerance. However, the extent of recovery varied among genotypes and those genotypes that fully recovered to rates of well-watered plants such as Benning and USDA-N8002 would seemingly be advantageous for drought conditions.}, number={6}, journal={Journal of Crop Improvement}, publisher={Informa UK Limited}, author={Rosas-Anderson, Pablo and Sinclair, Thomas R. and Locke, Anna and Carter, Thomas E. and Rufty, Thomas W.}, year={2020}, month={May}, pages={785–799} }
 @article{rosas-anderson_taggart_heitman_miller_sinclair_rufty_2018, title={Partitioning between evaporation and transpiration from Agrostis stolonifera L. during light and dark periods}, volume={260}, ISSN={["1873-2240"]}, DOI={10.1016/j.agrformet.2018.05.018}, abstractNote={Pressures on water availability for irrigation of turfgrasses continue in many parts of the United States as climate and weather patterns shift and populations increase. It is essential to understand underlying factors controlling water loss to more precisely predict irrigation requirements and develop new strategies for improving effective use of water. In this study, we investigate two key components of potential water loss from a bentgrass (Agrostis stolonifera L.) system that have not previously been examined in detail: 1) water loss in darkness, and 2) water loss through evaporation directly from the soil. The experiments were conducted in controlled environment chambers with intact cores from the field. An automated gravimetric system and soil moisture probes allowed precise measurements of water loss over ranges of vapor pressure deficits (VPD). The gravimetric and soil probe results indicated that substantial evapotranspiration occurred in darkness, at rates 40 to 60% of that in the light across VPDs. Simulations using field weather data from dry and humid environments indicated nighttime water loss rates would be expected to be 30 to 40% of that in the light. Using cores treated with a fast-acting, desiccating herbicide that eliminated transpiration but kept core resistances intact, evaporation directly from the soil surface was estimated to account for 40% of total water loss in the light and 60 to 70% in the dark. The results, collectively, indicated that water loss in darkness must be separately accounted for to accurately estimate daily evapotranspiration totals and irrigation requirements. Furthermore, because of the very high potential for evaporative water loss in the light and dark, efforts to improve water use efficiencies in the turfgrass system should include strategies that regulate both transpiration by the plant and evaporation from the soil surface.}, journal={AGRICULTURAL AND FOREST METEOROLOGY}, author={Rosas-Anderson, Pablo and Taggart, Matthew J. and Heitman, Joshua L. and Miller, Grady L. and Sinclair, Thomas R. and Rufty, Thomas W.}, year={2018}, month={Oct}, pages={73–79} }
 @article{sinclair_manandhar_shekoofa_rosas-anderson_bagherzadi_schoppach_sadok_rufty_2017, title={Pot binding as a variable confounding plant phenotype: theoretical derivation and experimental observations}, volume={245}, ISSN={["1432-2048"]}, DOI={10.1007/s00425-016-2641-0}, abstractNote={Theoretical derivation predicted growth retardation due to pot water limitations, i.e., pot binding. Experimental observations were consistent with these limitations. Combined, these results indicate a need for caution in high-throughput screening and phenotyping. Pot experiments are a mainstay in many plant studies, including the current emphasis on developing high-throughput, phenotyping systems. Pot studies can be vulnerable to decreased physiological activity of the plants particularly when pot volume is small, i.e., "pot binding". It is necessary to understand the conditions under which pot binding may exist to avoid the confounding influence of pot binding in interpreting experimental results. In this paper, a derivation is offered that gives well-defined conditions for the occurrence of pot binding based on restricted water availability. These results showed that not only are pot volume and plant size important variables, but the potting media is critical. Artificial potting mixtures used in many studies, including many high-throughput phenotyping systems, are particularly susceptible to the confounding influences of pot binding. Experimental studies for several crop species are presented that clearly show the existence of thresholds of plant leaf area at which various pot sizes and potting media result in the induction of pot binding even though there may be no immediate, visual plant symptoms. The derivation and experimental results showed that pot binding can readily occur in plant experiments if care is not given to have sufficiently large pots, suitable potting media, and maintenance of pot water status. Clear guidelines are provided for avoiding the confounding effects of water-limited pot binding in studying plant phenotype.}, number={4}, journal={PLANTA}, author={Sinclair, Thomas R. and Manandhar, Anju and Shekoofa, Avat and Rosas-Anderson, Pablo and Bagherzadi, Laleh and Schoppach, Remy and Sadok, Walid and Rufty, Thomas W.}, year={2017}, month={Apr}, pages={729–735} }
 @article{shekoofa_rosas-anderson_carley_sinclair_rufty_2016, title={Limited transpiration under high vapor pressure deficits of creeping bentgrass by application of Daconil-Action (R)}, volume={243}, ISSN={["1432-2048"]}, DOI={10.1007/s00425-015-2417-y}, abstractNote={First observation that chemical spray can induce limited-transpiration rate under high vapor pressure deficit. It appears that acibenzolar may be key in inducing this water conservation trait. Irrigation and water use have become major issues in management of turfgrasses. Plant health products that have been introduced into the turfgrass market have been observed to improve plant performance in water stress conditions. In this study, we evaluated whether a selection of common plant health products alter the ability of creeping bentgrass (Agrostis stolonifera L.) to control transpiration under high vapor pressure deficit (VPD). The plant health treatments--Daconil Action, Insignia, and Signature--were applied to plots on golf course putting greens located in Raleigh NC and in Scottsdale, AZ. Using intact cores removed from the putting greens, transpiration rates were measured over a range of VPDs in controlled conditions. In all cases stretching over a 3-year period, bentgrass cores from field plots treated with Daconil-Action limited transpiration under high VPD conditions, while check treatments with water, and others treated with Insignia or Signature did not. Transpiration control became engaged when VPDs reached values ranging from 1.39 to 2.50 kPa, and was not strongly influenced by the field temperature at which the bentgrass was growing. Because all plots in NC had been treated with chlorothalonil-the key ingredient in Daconil Action to control diseases-it was concluded that the likely chemical ingredient in Daconil Action triggering the transpiration control response was acibenzolar. This is the first evidence that the limited-transpiration trait can be induced by a chemical application, and it implies significant potential for ameliorating drought vulnerability in cool-season turfgrasses, and likely other plant species.}, number={2}, journal={PLANTA}, author={Shekoofa, Avat and Rosas-Anderson, Pablo and Carley, Danesha S. and Sinclair, Thomas R. and Rufty, Thomas W.}, year={2016}, month={Feb}, pages={421–427} }
 @article{shekoofa_rosas-anderson_sinclair_balota_isleib_2015, title={Measurement of Limited-Transpiration Trait under High Vapor Pressure Deficit for Peanut in Chambers and in Field}, volume={107}, ISSN={["1435-0645"]}, DOI={10.2134/agronj14.0570}, abstractNote={Drought is one of the most important environmental factors that limit crop production. Based on controlled‐environment studies, it has been hypothesized that a limited‐transpiration (TRlim) trait under high vapor pressure deficit (VPD) is a mechanism for water conservation leading to yield increase under water‐deficit conditions. The current research objective was to compare expression of TRlim in peanut (Arachis hypogaea L.) observed by whole‐plant measurements in controlled environments and by leaf gas exchange measurements on plants grown in the field. Six peanut genotypes with different breeding backgrounds, that is, wild‐type, commercial cultivars, and advanced breeding lines were studied. Differences were observed among genotypes in their expression of TRlim with increasing VPD in the controlled environment at 31/26°C. Within each breeding background, one genotype showed a linear increase in transpiration with increasing VPD while the other expressed the TRlim trait. In a second set of controlled environment experiments at 36/26°C, none of the six genotypes expressed the TRlim trait. In the field, again none of the genotypes expressed the TRlim trait. The temperature to which the plants were exposed between the two controlled environments and field trial appeared critical in the expression of the TRlim trait of three of the genotypes.}, number={3}, journal={AGRONOMY JOURNAL}, author={Shekoofa, Avat and Rosas-Anderson, Pablo and Sinclair, Thomas R. and Balota, Maria and Isleib, Thomas G.}, year={2015}, pages={1019–1024} }
 @article{rosas-anderson_sinclair_balota_tallury_isleib_rufty_2014, title={Genetic Variation for Epidermal Conductance in Peanut}, volume={54}, ISSN={["1435-0653"]}, DOI={10.2135/cropsci2013.07.0461}, abstractNote={ABSTRACTPeanut (Arachis hypogaea L.) is an important legume that is often grown in drought‐prone areas. Low epidermal conductance (EC) may delay the dehydration of leaves and improve plant survival of severe water‐deficit stress. No reports of genetic variation for EC in peanut have been found in the literature. Therefore, the main objective of this study was to document genetic variation for EC among 21 peanut (Arachis spp.) genotypes in two greenhouse experiments and a field experiment. The effects of water‐deficit stress on EC and the association between EC and stomata density (SD) were also investigated. Tests for an influence of water‐deficit stress on EC were inconclusive. Comparison of EC and SD among genotypes failed to show any significant correlations in the various test environments. However, genetic variation for EC in peanut was found in both greenhouse experiments, with genotypes TMV 2, PI 298639, and VA 98R having very low EC. In the field, genotype SPT 06‐07 was found to have low EC. These results indicate that selection of genotypes with low EC is a viable approach in identifying peanut genotypes with improved capacity to survive severe soil water deficits.}, number={2}, journal={CROP SCIENCE}, author={Rosas-Anderson, Pablo and Sinclair, Thomas R. and Balota, Maria and Tallury, Shyam and Isleib, Thomas G. and Rufty, Thomas}, year={2014}, pages={730–737} }
 @article{tallury_isleib_copeland_rosas-anderson_balota_singh_stalker_2014, title={Registration of Two Multiple Disease-Resistant Peanut Germplasm Lines Derived from Arachis cardenasii Krapov. & WC Gregory, GKP 10017}, volume={8}, ISSN={["1940-3496"]}, DOI={10.3198/jpr2013.04.0017crg}, abstractNote={Journal of Plant RegistrationsVolume 8, Issue 1 p. 86-89 Germplasm Registration of Two Multiple Disease-Resistant Peanut Germplasm Lines Derived from Arachis cardenasii Krapov. & W.C. Gregory, GKP 10017 S. P. Tallury, Corresponding Author S. P. Tallury stallur@clemson.edu Dep. of Crop Science, Box 7629, North Carolina State Univ., Raleigh, NC, 27695-7629Corresponding author (stallur@clemson.edu)Search for more papers by this authorT. G. Isleib, T. G. Isleib Dep. of Crop Science, Box 7629, North Carolina State Univ., Raleigh, NC, 27695-7629Search for more papers by this authorS. C. Copeland, S. C. Copeland Dep. of Crop Science, Box 7629, North Carolina State Univ., Raleigh, NC, 27695-7629Search for more papers by this authorP. Rosas-Anderson, P. Rosas-Anderson Dep. of Crop Science, Box 7629, North Carolina State Univ., Raleigh, NC, 27695-7629Search for more papers by this authorM. Balota, M. Balota Virginia Tech, Blacksburg, VASearch for more papers by this authorD. Singh, D. Singh Virginia Tech, Blacksburg, VASearch for more papers by this authorH. T. Stalker, H. T. Stalker Dep. of Crop Science, Box 7629, North Carolina State Univ., Raleigh, NC, 27695-7629Search for more papers by this author S. P. Tallury, Corresponding Author S. P. Tallury stallur@clemson.edu Dep. of Crop Science, Box 7629, North Carolina State Univ., Raleigh, NC, 27695-7629Corresponding author (stallur@clemson.edu)Search for more papers by this authorT. G. Isleib, T. G. Isleib Dep. of Crop Science, Box 7629, North Carolina State Univ., Raleigh, NC, 27695-7629Search for more papers by this authorS. C. Copeland, S. C. Copeland Dep. of Crop Science, Box 7629, North Carolina State Univ., Raleigh, NC, 27695-7629Search for more papers by this authorP. Rosas-Anderson, P. Rosas-Anderson Dep. of Crop Science, Box 7629, North Carolina State Univ., Raleigh, NC, 27695-7629Search for more papers by this authorM. Balota, M. Balota Virginia Tech, Blacksburg, VASearch for more papers by this authorD. Singh, D. Singh Virginia Tech, Blacksburg, VASearch for more papers by this authorH. T. Stalker, H. T. Stalker Dep. of Crop Science, Box 7629, North Carolina State Univ., Raleigh, NC, 27695-7629Search for more papers by this author First published: 19 December 2013 https://doi.org/10.3198/jpr2013.04.0017crgCitations: 31 All rights reserved. No part of this periodical may be reproduced or transmitted in any form or by any means, electronic or mechanical, including photocopying, recording, or any information storage and retrieval system, without permission in writing from the publisher. Permission for printing and for reprinting the material contained herein has been obtained by the publisher. Read the full textAboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onFacebookTwitterLinkedInRedditWechat Abstract Two tetraploid (2n = 4x = 40) peanut (Arachis hypogaea L. subsp. hypogaea var. hypogaea) germplasm lines, GP-NC WS 16 (SPT 06-06) (Reg. No. GP-235, PI 669445) and GP-NC WS 17 (SPT 06-07) (Reg. No. GP-236, PI 669446), derived from interspecific hybridization, were developed in the peanut genetics program at North Carolina State University (NCSU), Raleigh, NC. These two lines were tested extensively by the North Carolina Agricultural Research Service from 2006 through 2012 in disease evaluation tests. They have unique alleles introgressed from the diploid (2n = 2x = 20) wild species, A. cardenasii Krapov. & W.C. Gregory. The germplasm lines are also unique in that they exhibited multiple disease resistances superior to the germplasm lines derived from A. cardenasii that were released previously by NCSU. Resistance to multiple diseases included early leaf spot (ELS), Cylindrocladium black rot (CBR), Sclerotinia blight (SB), and tomato spotted wilt (TSW). One of the lines, GP-NC WS 17, also exhibited drought tolerance in field and greenhouse studies. Thus, it can be concluded that these two peanut germplasm lines derived from diploid wild species have multiple biotic stress resistances, specifically for ELS, CBR, SB, and TSWV, as well as abiotic stress resistance in the case of GP-NC WS 17. These two lines should provide unique, improved germplasm for breeders interested in multiple disease resistance and in expanding the germplasm pool of A. hypogaea. Citing Literature Volume8, Issue1January 2014Pages 86-89 RelatedInformation}, number={1}, journal={JOURNAL OF PLANT REGISTRATIONS}, author={Tallury, S. P. and Isleib, T. G. and Copeland, S. C. and Rosas-Anderson, P. and Balota, M. and Singh, D. and Stalker, H. T.}, year={2014}, month={Jan}, pages={86–89} }