@article{mcculloch_mente_o’nan_ashwell_2018, title={Articular cartilage gene expression patterns in the tissue surrounding the impact site following applications of shear and axial loads}, volume={19}, ISSN={1471-2474}, url={http://dx.doi.org/10.1186/s12891-018-2374-2}, DOI={10.1186/s12891-018-2374-2}, abstractNote={Osteoarthritis is a degradative joint disease found in humans and commercial swine which can develop from a number of factors, including prior joint trauma. An impact injury model was developed to deliver in vitro loads to disease-free porcine patellae in a model of OA.Axial impactions (2000 N normal) and shear impactions (500 N normal with induced shear forces) were delivered to 48 randomly assigned patellae. The patellae were then cultured for 0, 3, 7, or 14 days following the impact. Specimens in the tissue surrounding the loading site were harvested and expression of 18 OA related genes was studied via quantitative PCR. The selected genes were previously identified from published work and fell into four categories: cartilage matrix, degradative enzymes, inflammatory response, and apoptosis.Type II collagen (Col2a1) showed significantly lower expression in shear vs. axial adjacent tissue at day 0 and 7 (fold changes of 0.40 & 0.19, respectively). In addition, higher expression of degradative enzymes and Fas, an apoptosis gene, was observed in the shear specimens.The results suggest that a more physiologically valid shear load may induce more damage to surrounding articular cartilage than a normal load alone.}, number={1}, journal={BMC Musculoskeletal Disorders}, publisher={Springer Nature}, author={McCulloch, R. S. and Mente, P. L. and O’Nan, A. T. and Ashwell, M. S.}, year={2018}, month={Dec} }
 @article{early_mente_dillard_roe_2015, title={In vitro biomechanical evaluation of internal fixation techniques on the canine lumbosacral junction}, volume={3}, journal={PeerJ}, author={Early, P. and Mente, P. and Dillard, S. and Roe, S.}, year={2015} }
 @article{hong_evans_mente_2015, title={Study on the damage mechanism of articular cartilage based on the fluid-solid coupled particle model}, volume={7}, number={4}, journal={Advances in Mechanical Engineering}, author={Hong, J. and Evans, T. M. and Mente, P. L.}, year={2015} }
 @article{ashwell_gonda_gray_maltecca_audrey t. o'nan_cassady_mente_2013, title={Changes in chondrocyte gene expression following in vitro impaction of porcine articular cartilage in an impact injury model}, volume={31}, ISSN={["1554-527X"]}, url={http://www.scopus.com/inward/record.url?eid=2-s2.0-84872761673&partnerID=MN8TOARS}, DOI={10.1002/jor.22239}, abstractNote={Abstract}, number={3}, journal={JOURNAL OF ORTHOPAEDIC RESEARCH}, author={Ashwell, Melissa S. and Gonda, Michael G. and Gray, Kent and Maltecca, Christian and Audrey T. O'Nan and Cassady, Joseph P. and Mente, Peter L.}, year={2013}, month={Mar}, pages={385–391} }
 @article{arnold_mathews_roe_mente_seaboch_2009, title={Biomechanical Comparison of Four Soft Tissue Replacement Materials: An In Vitro Evaluation of Single and Multilaminate Porcine Small Intestinal Submucosa, Canine Fascia Lata, and Polypropylene Mesh}, volume={38}, ISSN={["1532-950X"]}, DOI={10.1111/j.1532-950X.2009.00577.x}, abstractNote={Objective— To compare mechanical performance of 4 soft tissue replacement materials.}, number={7}, journal={VETERINARY SURGERY}, author={Arnold, Gregory A. and Mathews, Kyle G. and Roe, Simon and Mente, Peter and Seaboch, Tim}, year={2009}, month={Oct}, pages={834–844} }
 @article{ferket_oviedo-rondon_mente_bohorquez_santos_grimes_richards_dibner_felts_2009, title={Organic trace minerals and 25-hydroxycholecalciferol affect performance characteristics, leg abnormalities, and biomechanical properties of leg bones of turkeys}, volume={88}, ISSN={["1525-3171"]}, DOI={10.3382/ps.2008-00200}, abstractNote={Leg problems and resulting mortality can exceed 1% per week in turkey toms starting at approximately 15 wk of age. Dietary supplementation of organic trace minerals (MIN) and 25-hydroxycholecalciferol (HyD) may improve performance, decrease incidence of leg abnormalities, and increase bone strength. Nicholas 85X700 toms were assigned to 4 treatments consisting of a factorial arrangement of 2 concentrations of MIN (0 and 0.1% of Mintrex P(Se), which adds 40, 40, 20, and 0.3 mg/kg of Zn, Mn, Cu, and Se, respectively) and 2 concentrations of HyD (0 and 92 microg/kg of HyD). Diets were formulated to be equal in nutrient content and fed ad libitum as 8 feed phases. Feed intake and BW were measured at 6, 12, 15, 17, and 20 wk of age. Valgus, varus, and shaky leg defects were determined at 12, 15, 17, and 20 wk of age. Tibia and femur biomechanical properties were evaluated by torsion and bending tests at 17 wk of age. There were no treatment effects on BW. Only MIN significantly improved feed conversion ratio through to 20 wk of age. Cumulative mortality at 3 wk of age was greater among the MIN birds, but it was lower by 20 wk (P = 0.085). The MIN decreased the incidence of varus defects at 17 wk of age; shaky leg at 12, 15, and 17 wk of age; and valgus defects at 15, 17, and 20 wk of age. There were no MIN x HyD interaction effects on individual gait problems. Maximum load and the bending stress required for tibias to break in a 4-point assay were increased with MIN supplementation, especially when HyD was also added. Maximum shear stress at failure of femoral bones in a torsion assay was increased by supplementation with both MIN and HyD together. Dietary supplementation of MIN and HyD may improve biomechanical properties of bones. Dietary MIN supplementation may improve feed conversion of turkeys, likely by decreasing leg problems.}, number={1}, journal={POULTRY SCIENCE}, author={Ferket, P. R. and Oviedo-Rondon, E. O. and Mente, P. L. and Bohorquez, D. V. and Santos, A. A., Jr. and Grimes, J. L. and Richards, J. D. and Dibner, J. J. and Felts, V.}, year={2009}, month={Jan}, pages={118–131} }
 @article{ashwell_o'nan_gonda_mente_2008, title={Gene expression profiling of chondrocytes from a porcine impact injury model}, volume={16}, ISSN={["1522-9653"]}, DOI={10.1016/j.joca.2007.12.012}, abstractNote={ObjectiveTo identify differentially expressed genes between axially impacted and control articular cartilage taken from porcine patellae maintained in organ culture for 14 days.MethodsPorcine patellae were impacted perpendicular to the articular surface to create an impact injury. Intact patellae (control and impacted) were maintained in culture for 14 days. Total RNA was then extracted from the articular cartilage beneath the impaction and used to prepare two Serial Analysis of Gene Expression (SAGE) libraries. Approximately 42,500 SAGE long tags were sequenced from the libraries. The expression of select genes was confirmed by quantitative real-time PCR analysis.ResultsThirty-nine SAGE tags were significantly differentially expressed in the impacted and control libraries, representing 30 different annotated pig genes. These genes represented gene products associated with matrix molecules, iron and phosphate transport, protein biosynthesis, skeletal development, cell proliferation, lipid metabolism and the inflammatory response. Twenty-three of the 30 genes were down-regulated in the impacted library and five were up-regulated in the impacted library. Quantitative real-time PCR follow-up of four genes supported the results found with SAGE.ConclusionWe have identified 30 putative genes differentially expressed in a porcine impact injury model and validated these findings for four of these genes using real-time PCR. Results using this impact injury model have contributed further evidence that damaged chondrocytes may de-differentiate into fibroblast-like cells and proliferate in an attempt to repair themselves. Additional work is underway to study these genes in further detail at earlier time points to provide a more complete story about the fate of chondrocytes in articular cartilage following an injury.}, number={8}, journal={OSTEOARTHRITIS AND CARTILAGE}, author={Ashwell, M. S. and O'Nan, A. T. and Gonda, M. G. and Mente, P. L.}, year={2008}, month={Aug}, pages={936–946} }
 @article{ovsianikov_chichkov_mente_monteiro-riviere_doraiswamy_narayan_2007, title={Two photon polymerization of polymer-ceramic hybrid materials for transdermal drug delivery}, volume={4}, ISSN={["1744-7402"]}, url={http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=ORCID&SrcApp=OrcidOrg&DestLinkType=FullRecord&DestApp=WOS_CPL&KeyUT=WOS:000243791400003&KeyUID=WOS:000243791400003}, DOI={10.1111/j.1744-7402.2007.02115.x}, abstractNote={Three‐dimensional microneedle devices were created by femtosecond laser two photon polymerization (2PP) of organically modified ceramic (Ormocer®) hybrid materials. Arrays of in‐plane and out‐of‐plane hollow microneedles (microneedle length=800 μm, microneedle base diameter=150–300 μm) with various aspect ratios were fabricated. The fracture and penetration properties of the microneedle arrays were examined using compression load testing. In these studies, the microneedle arrays penetrated cadaveric porcine adipose tissue without fracture. Human epidermal keratinocyte viability on the Ormocer® surfaces polymerized using 2PP was similar to that on control surfaces. These results suggest that 2PP is able to create microneedle structures for transdermal drug delivery with a larger range of geometries than conventional microfabrication techniques.}, number={1}, journal={INTERNATIONAL JOURNAL OF APPLIED CERAMIC TECHNOLOGY}, author={Ovsianikov, A. and Chichkov, B. and Mente, P. and Monteiro-Riviere, N. A. and Doraiswamy, A. and Narayan, R. J.}, year={2007}, pages={22–29} }
 @article{davis_roe_mathews_mente_2006, title={Median sternotomy closure in dogs: A mechanical comparison of technique stability}, volume={35}, ISSN={["0161-3499"]}, DOI={10.1111/j.1532-950X.2006.00143.x}, abstractNote={Objective— To evaluate the mechanical properties of canine median sternotomy closure using double‐loop cerclage compared with other commonly used cerclage sternotomy closure patterns.}, number={3}, journal={VETERINARY SURGERY}, author={Davis, KM and Roe, SC and Mathews, KG and Mente, PL}, year={2006}, month={Apr}, pages={271–277} }
 @article{doraiswamy_jin_narayan_mageswaran_mente_modi_auyeung_chrisey_ovsianikov_chichkov_2006, title={Two photon induced polymerization of organic-inorganic hybrid biomaterials for microstructured medical devices}, volume={2}, ISSN={["1878-7568"]}, DOI={10.1016/j.actbio.2006.01.004}, abstractNote={Three-dimensional microstructured medical devices, including microneedles and tissue engineering scaffolds, were fabricated by two photon induced polymerization of Ormocer® organic–inorganic hybrid materials. Femtosecond laser pulses from a titanium:sapphire laser were used to break chemical bonds on Irgacure® 369 photoinitiator within a small focal volume. The radicalized starter molecules reacted with Ormocer® US-S4 monomers to create radicalized polymolecules. The desired structures are fabricated by moving the laser focus in three dimensions using a galvano-scanner and a micropositioning system. Ormocer® surfaces fabricated using two photon induced polymerization demonstrated acceptable cell viability and cell growth profiles against B35 neuroblast-like cells and HT1080 epithelial-like cells. Lego®-like interlocking tissue engineering scaffolds and microneedle arrays with unique geometries were created using two photon induced polymerization. These results suggest that two photon induced polymerization is able to create medical microdevices with a larger range of sizes, shapes, and materials than chemical isotropic etching, injection molding, reactive ion etching, surface micromachining, bulk micromachining, polysilicon micromolding, lithography–electroforming–replication, or other conventional microfabrication techniques.}, number={3}, journal={ACTA BIOMATERIALIA}, author={Doraiswamy, A and Jin, C and Narayan, RJ and Mageswaran, P and Mente, P and Modi, R and Auyeung, R and Chrisey, DB and Ovsianikov, A and Chichkov, B}, year={2006}, month={May}, pages={267–275} }
 @article{mathews_roe_stebbins_barnes_mente_2004, title={Biomechanical evaluation of suture pullout from canine arytenoid cartilages: Effects of hole diameter, suture configuration, suture size, and distraction rate}, volume={33}, ISSN={["0161-3499"]}, DOI={10.1111/j.1532-950X.2004.04029.x}, abstractNote={Objective— To evaluate the mechanical properties of canine arytenoid cartilage–suture constructs.}, number={3}, journal={VETERINARY SURGERY}, author={Mathews, KG and Roe, S and Stebbins, M and Barnes, R and Mente, PL}, year={2004}, pages={191–199} }
 @inproceedings{mccord_blanchard_mente_nagle_spurlin_2003, title={Outcomes based curriculum development in a new and emerging biomedical engineering program}, booktitle={American Society for Engineering Education}, author={McCord, M. G. and Blanchard, S. M. and Mente, P. L. and Nagle, H. T. and Spurlin, J. E.}, year={2003} }