@article{silva_almeida_michael_rahe_siepker_magstadt_piñeyro_arruda_macedo_sahin_et al._2023, title={Detection and disease diagnosis trends (2017–2022) for Streptococcus suis, Glaesserella parasuis, Mycoplasma hyorhinis, Actinobacillus suis and Mycoplasma hyosynoviae at Iowa State University Veterinary Diagnostic Laboratory}, url={http://dx.doi.org/10.1186/s12917-023-03807-w}, DOI={10.1186/s12917-023-03807-w}, abstractNote={Abstract}, journal={BMC Veterinary Research}, author={Silva, Ana Paula Serafini Poeta and Almeida, Marcelo and Michael, Alyona and Rahe, Michael C. and Siepker, Christopher and Magstadt, Drew R. and Piñeyro, Pablo and Arruda, Bailey L. and Macedo, Nubia R. and Sahin, Orhan and et al.}, year={2023}, month={Dec} }
 @article{chen_mou_lu_schumacher_resende-de-macedo_sitthicharoenchai_derscheid_burrough_li_2023, title={Genomic characterization of <i>Streptococcus equi</i> subspecies <i>zooepidemicus</i> from a 2021 outbreak in Indiana with increased sow mortality}, volume={10}, ISSN={["2379-5042"]}, DOI={10.1128/msphere.00404-23}, abstractNote={ABSTRACT}, journal={MSPHERE}, author={Chen, Xuhua and Mou, Kathy and Lu, Weili and Schumacher, Loni and Resende-De-Macedo, Nubia and Sitthicharoenchai, Panchan and Derscheid, Rachel and Burrough, Eric and Li, Ganwu}, year={2023}, month={Oct} }
 @article{hau_lantz_stuart_sitthicharoenchai_macedo_derscheid_burrough_robbe-austerman_brockmeier_2022, title={Replication of Streptococcus equi subspecies zooepidemicus infection in swine}, volume={264}, url={https://doi.org/10.1016/j.vetmic.2021.109271}, DOI={10.1016/j.vetmic.2021.109271}, abstractNote={Streptococcus equi subspecies zooepidemicus (SEZ) is a commensal bacterium of horses and causes infections in mammalian species, including humans. Historically, virulent strains of SEZ caused high mortality in pigs in China and Indonesia, while disease in the U.S. was infrequent. More recently, high mortality events in sows were attributed to SEZ in North America. The SEZ isolates from these mortality events have high genetic similarity to an isolate from an outbreak in China. Taken together, this may indicate SEZ is an emerging threat to swine health. To generate a disease model and evaluate the susceptibility of healthy, conventionally raised pigs to SEZ, we challenged sows and five-month-old pigs with an isolate from a 2019 mortality event. Pigs were challenged with a genetically similar guinea pig isolate or genetically distinct horse isolate to evaluate comparative virulence. The swine isolate caused severe systemic disease in challenged pigs with 100 % mortality. Disease manifestation in sows was similar to field reports: lethargy/depression, fever, reluctance to rise, and high mortality. The guinea pig isolate also caused severe systemic disease; however, most five-month-old pigs recovered. In contrast, the horse isolate did not cause disease and was readily cleared from the respiratory tract. In conclusion, we were able to replicate disease reported in the field. The results indicate differences in virulence between isolates, with the highest virulence associated with the swine isolate. Additionally, we generated a challenge model that can be used in future research to evaluate virulence factors and disease prevention strategies.}, journal={Veterinary Microbiology}, publisher={Elsevier BV}, author={Hau, Samantha J. and Lantz, Kristina and Stuart, Keira L. and Sitthicharoenchai, Panchan and Macedo, Nubia and Derscheid, Rachel J. and Burrough, Eric R. and Robbe-Austerman, Suelee and Brockmeier, Susan L.}, year={2022}, month={Jan}, pages={109271} }
 @article{sitthicharoenchai_burrough_arruda_sahin_santos_magstadt_piñeyro_schwartz_rahe_2022, title={Streptococcus gallolyticus and Bacterial Endocarditis in Swine, United States, 2015-2020.}, volume={28}, url={https://europepmc.org/articles/PMC8714216}, DOI={10.3201/eid2801.210998}, abstractNote={To evaluate trends in bacterial causes of valvular endocarditis in swine, we retrospectively analyzed 321 cases diagnosed at Iowa State University Veterinary Diagnostic Laboratory (Ames, IA, USA) during May 2015–April 2020. Streptococcus gallolyticus was the causative agent for 7.59% of cases. This emerging infection in swine could aid study of endocarditis in humans.}, number={1}, journal={Emerging infectious diseases}, publisher={Centers for Disease Control and Prevention (CDC)}, author={Sitthicharoenchai, Panchan and Burrough, Eric R. and Arruda, Bailey L. and Sahin, Orhan and Santos, Jessica G. and Magstadt, Drew R. and Piñeyro, Pablo E. and Schwartz, Kent J. and Rahe, Michael C.}, year={2022}, month={Jan}, pages={192–195} }
 @article{sitthicharoenchai_alnajjar_ackermann_2020, title={A model of respiratory syncytial virus (RSV) infection of infants in newborn lambs}, volume={380}, url={http://www.scopus.com/inward/record.url?eid=2-s2.0-85083966556&partnerID=MN8TOARS}, DOI={10.1007/s00441-020-03213-w}, abstractNote={Many animal models have been established for respiratory syncytial virus (RSV) infection of infants with the purpose of studying the pathogenesis, immunological response, and pharmaceutical testing and the objective of finding novel therapies and preventive measures. This review centers on a neonatal lamb model of RSV infection that has similarities to RSV infection of infants. It includes a comprehensive description of anatomical and immunological similarities between ovine and human lungs along with comparison of pulmonary changes and immune responses with RSV infection. These features make the newborn lamb an effective model for investigating key aspects of RSV infection in infants. The importance of RSV lamb model application in preclinical therapeutic trials and current updates on new studies with the RSV-infected neonatal lamb are also highlighted.}, number={2}, journal={Cell and Tissue Research}, author={Sitthicharoenchai, P. and Alnajjar, S. and Ackermann, M.R.}, year={2020}, pages={313–324} }
 @article{sitthicharoenchai_derscheid_schwartz_macedo_sahin_chen_li_main_burrough_2020, title={Cases of high mortality in cull sows and feeder pigs associated with Streptococcus equi subsp. zooepidemicus septicemia}, url={https://doi.org/10.1177/1040638720927669}, DOI={10.1177/1040638720927669}, abstractNote={ Investigations of 2 cases of high mortality in cull sows and feeder pigs from a buying station in Ohio and cull sows at an abattoir in Tennessee were conducted at the Iowa State University Veterinary Diagnostic Laboratory. The animals were presented as weak, lethargic, and some with high fever. Rapidly escalating mortality was reported to be as high as 30–50% within groups at the buying station over 8–10 d, and 30–40% over 5–7 d at the abattoir. Splenomegaly and red lymph nodes were the most consistent macroscopic findings, with scant fibrinous polyserositis observed in one sow. The microscopic lesions of vasculitis, fibrin thrombi, fibrinosuppurative polyserositis, and intralesional bacteria were consistent with acute bacterial septicemia. Bacterial culture isolated Streptococcus equi subsp. zooepidemicus ( S. zooepidemicus) from multiple organs, including spleen, lung, and kidney. PCR tests were negative for African swine fever virus, classical swine fever virus, Erysipelothrix rhusiopathiae, porcine reproductive and respiratory syndrome virus, porcine circovirus 2, and Salmonella spp. Porcine circovirus 3 was inconsistently detected at low levels by PCR, with a lack of associated lesions. Next-generation sequencing identified S. zooepidemicus and porcine partetravirus in the serum sample of the feeder pig from the buying station. Phylogenetic analysis of the szP gene indicated that the S. zooepidemicus isolates from Ohio and Tennessee are in genotype VI. We conclude that the cause of these high mortality events in swine was S. zooepidemicus septicemia. }, journal={Journal of Veterinary Diagnostic Investigation}, author={Sitthicharoenchai, Panchan and Derscheid, Rachel and Schwartz, Kent and Macedo, Nubia and Sahin, Orhan and Chen, Xuhua and Li, Ganwu and Main, Rodger and Burrough, Eric}, year={2020}, month={Jul} }
 @article{chen_resende‐de‐macedo_sitthicharoenchai_sahin_burrough_clavijo_derscheid_schwartz_lantz_robbe‐austerman_et al._2020, title={Genetic characterization of Streptococcus equi subspecies zooepidemicus associated with high swine mortality in the United States}, volume={6}, url={https://doi.org/10.1111/tbed.13645}, DOI={10.1111/tbed.13645}, abstractNote={High mortality events due to Streptococcus equi subspecies zooepidemicus (Streptococcus zooepidemicus) in swine have not previously been reported in the United States. In September and October 2019, outbreaks with swine mortality up to 50% due to S. zooepidemicus septicaemia were reported in Ohio and Tennessee. Genomic epidemiological analysis revealed that the eight outbreak isolates were clustered together with ATCC 35246, a Chinese strain caused outbreaks with high mortality, also closely related to three isolates from human cases from Virginia, but significantly different from an outbreak-unrelated swine isolate from Arizona and most isolates from other animal species. Comparative genomic analysis on two outbreak isolates and another outbreak-unrelated isolate identified several genomic islands and virulence genes specifically in the outbreak isolates only, which are likely associated with the high mortality observed in the swine population. These findings have implications for understanding, tracking and possibly preventing diseases caused by S. zooepidemicus in swine.}, journal={Transboundary and Emerging Diseases}, publisher={Wiley}, author={Chen, Xuhua and Resende‐De‐Macedo, Nubia and Sitthicharoenchai, Panchan and Sahin, Orhan and Burrough, Eric and Clavijo, Maria and Derscheid, Rachel and Schwartz, Kent and Lantz, Kristina and Robbe‐Austerman, Suelee and et al.}, year={2020}, month={Nov} }
 @article{sitthicharoenchai_woonwong_poonsuk_arunorat_muangpaisarn_samatiwat_konthong_sattathara_thanawongnuwech_2018, title={Evaluation of a commercial ELISA test kit on classical swine fever antibody detection using oral fluid samples}, volume={48}, url={http://www.scopus.com/inward/record.url?eid=2-s2.0-85055167509&partnerID=MN8TOARS}, number={3}, journal={Thai Journal of Veterinary Medicine}, author={Sitthicharoenchai, P. and Woonwong, Y. and Poonsuk, K. and Arunorat, J. and Muangpaisarn, C. and Samatiwat, K. and Konthong, W. and Sattathara, W. and Thanawongnuwech, R.}, year={2018}, pages={463–469} }
 @article{detection of actinobacillus pleuropneumoniae apxiv toxin antibody in serum and oral fluid specimens from pigs inoculated under experimental conditions._2017, url={http://europepmc.org/articles/PMC5894388}, DOI={10.1515/jvetres-2017-0021}, abstractNote={Abstract}, journal={Journal of veterinary research}, year={2017}, month={Jun} }
 @article{protection of human influenza vaccines against a reassortant swine influenza virus of pandemic h1n1 origin using a pig model._2017, url={https://doi.org/10.1016/j.rvsc.2017.02.022}, DOI={10.1016/j.rvsc.2017.02.022}, abstractNote={Since the pandemic H1N1 emergence in 2009 (pdmH1N1), many reassortant pdmH1N1 viruses emerged and found circulating in the pig population worldwide. Currently, commercial human subunit vaccines are used commonly to prevent the influenza symptom based on the WHO recommendation. In case of current reassortant swine influenza viruses transmitting from pigs to humans, the efficacy of current human influenza vaccines is of interest. In this study, influenza A negative pigs were vaccinated with selected commercial human subunit vaccines and challenged with rH3N2. All sera were tested with both HI and SN assays using four representative viruses from the surveillance data in 2012 (enH1N1, pdmH1N1, rH1N2 and rH3N2). The results showed no significant differences in clinical signs and macroscopic and microscopic findings among groups. However, all pig sera from vaccinated groups had protective HI titers to the enH1N1, pdmH1N1 and rH1N2 at 21 DPV onward and had protective SN titers only to pdmH1N1and rH1N2 at 21 DPV onward. SN test results appeared more specific than those of HI tests. All tested sera had no cross-reactivity against the rH3N2. Both studied human subunit vaccines failed to protect and to stop viral shedding with no evidence of serological reaction against rH3N2. SIV surveillance is essential for monitoring a novel SIV emergence potentially for zoonosis.}, journal={Research in veterinary science}, year={2017}, month={Feb} }
 @article{therapeutic efficacy of a respiratory syncytial virus fusion inhibitor._2017, url={http://europepmc.org/articles/PMC5537225}, DOI={10.1038/s41467-017-00170-x}, abstractNote={Abstract}, journal={Nature communications}, year={2017}, month={Aug} }
 @article{charoenchanikran_kedkovid_sirisereewan_woonwong_arunorat_sitthichareonchai_sopipan_jittimanee_kesdangsakonwut_thanawongnuwech_2016, title={Efficacy of Fostera® PRRS modified live virus (MLV) vaccination strategy against a Thai highly pathogenic porcine reproductive and respiratory syndrome virus (HP-PRRSV) infection}, volume={48}, url={http://dx.doi.org/10.1007/s11250-016-1099-1}, DOI={10.1007/s11250-016-1099-1}, abstractNote={Recently, the Chinese highly pathogenic porcine reproductive and respiratory syndrome virus (PRRSV) (HP-PRRSV) belonging to lineage 8 causes severe symptom with high morbidity and high mortality rates to the Asian pig industry. A recent study showed that pigs immunized with Fostera® PRRS modified live virus (MLV) of lineage 8 could provide a degree of protection against a Vietnamese HP-PRRSV infection. It should be noted that PRRSV commonly found after weaning causes porcine respiratory disease complex (PRDC). Vaccination strategy should be evaluated in each farm scenario. Eighty-one PRRSV-free piglets obtained from a PRRS-free herd were divided into two experiments with the major difference of infection timing after vaccination, 42 days in experiment 1 (n = 42) and 28 days in experiment 2 (n = 39). Each experiment had similar protocol containing three groups including a negative control, unvaccinated challenged, and vaccinated challenged groups. Pigs in vaccination groups were immunized with Fostera® PRRS MLV vaccine at 3 weeks of age. Then, unvaccinated challenged and vaccinated challenged groups were intranasally inoculated with a Thai HP-PRRSV (10PL01). Vaccinated challenged pigs showed significantly lower levels of mean rectal temperatures, clinical severity, lung lesion scores, and viral titers in serum and lung tissue compared to the unvaccinated challenged pigs (p < 0.05). Vaccinated challenged pigs had higher survival rate than those of unvaccinated challenged pigs in both experiments. It should be noted that pigs challenged 42 days after vaccination showed a better performance than pigs challenged 28 days after vaccination. In conclusion, Fostera® PRRS MLV vaccine was able to improve the survival rate against the Thai HP-PRRSV infection in both 42- and 28-day vaccination-to-infection protocols.}, number={7}, journal={Tropical Animal Health and Production}, publisher={Springer Science and Business Media LLC}, author={Charoenchanikran, Ponlakrit and Kedkovid, Roongtham and Sirisereewan, Chaitawat and Woonwong, Yonlayong and Arunorat, Jirapat and Sitthichareonchai, Panchan and Sopipan, Natthawan and Jittimanee, Suphattra and Kesdangsakonwut, Sawang and Thanawongnuwech, Roongroje}, year={2016}, month={Oct}, pages={1351–1359} }
 @article{rungsipipat_sitthicharoenchai_marlow_prutthithaworn_tangkawattana_2016, title={Expression of metallothionein protein relating to proliferative cell index in malignant feline mammary tumors using high throughput tissue microarray technique}, volume={25}, url={http://www.scopus.com/inward/record.url?eid=2-s2.0-84959529094&partnerID=MN8TOARS}, DOI={10.1007/s00580-015-2208-7}, number={2}, journal={Comparative Clinical Pathology}, author={Rungsipipat, A. and Sitthicharoenchai, P. and Marlow, P. and Prutthithaworn, P. and Tangkawattana, S.}, year={2016}, pages={449–457} }
 @article{poonsuk_arunorat_woonwong_sitthicharoenchai_jittimanee_choojai_thanawongnuwech_2014, title={Antiviral activity of four commercial tilmicosin preparations against porcine reproductive and respiratory syndrome virus (PRRSV): An in vitro study}, volume={44}, url={http://www.scopus.com/inward/record.url?eid=2-s2.0-84911933196&partnerID=MN8TOARS}, number={2}, journal={Thai Journal of Veterinary Medicine}, author={Poonsuk, K. and Arunorat, J. and Woonwong, Y. and Sitthicharoenchai, P. and Jittimanee, S. and Choojai, P. and Thanawongnuwech, R.}, year={2014}, pages={217–222} }