Paul T Hamilton

Also known as: Paul T. Hamilton

Works (37)

Updated: April 4th, 2024 16:00

2021 article

Remodeling hydrogen bond interactions results in relaxed specificity of Caspase-3

Yao, L., Swartz, P., Hamilton, P. T., & Clark, A. C. (2021, January 1). Bioscience Reports, Vol. 41.

By: L. Yao *, P. Swartz*, P. Hamilton n  & A. Clark *

MeSH headings : Amino Acid Sequence; Animals; Aspartic Acid / metabolism; Caspase 3 / chemistry; Caspase 3 / metabolism; Caspase 6 / metabolism; Humans; Hydrogen Bonding; Sequence Homology, Amino Acid; Substrate Specificity; Valine / metabolism; Zebrafish; Zebrafish Proteins / chemistry; Zebrafish Proteins / metabolism
topics (OpenAlex): ATP Synthase and ATPases Research; Cell death mechanisms and regulation; Protein Structure and Dynamics
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Sources: Web Of Science, NC State University Libraries
Added: April 12, 2021

2020 article

Caspases from scleractinian coral show unique regulatory features

Shrestha, S., Tung, J., Grinshpon, R. D., Swartz, P., Hamilton, P. T., Dimos, B., … Clark, A. C. (2020, August 12). Journal of Biological Chemistry, Vol. 295, pp. 14578–14591.

By: S. Shrestha *, J. Tung *, R. Grinshpon n, P. Swartz n, P. Hamilton n , B. Dimos *, L. Mydlarz *, A. Clark*

author keywords: caspase; cysteine protease; allosteric regulation; substrate specificity; coral apoptosis; coral immunity; functional divergence; substrate selection; CARD-caspase; apoptosis
MeSH headings : Amino Acid Sequence; Animals; Anthozoa / chemistry; Anthozoa / cytology; Anthozoa / enzymology; Anthozoa / metabolism; Apoptosis; Caspases / chemistry; Caspases / metabolism; Coral Reefs; Crystallography, X-Ray; Enzyme Activation; Humans; Models, Molecular; Protein Conformation; Protein Domains; Sequence Alignment; Substrate Specificity
topics (OpenAlex): Coral and Marine Ecosystems Studies; Aquaculture disease management and microbiota; Vibrio bacteria research studies
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14. Life Below Water (OpenAlex)
Sources: ORCID, Web Of Science, NC State University Libraries
Added: January 14, 2021

2019 article

Resurrection of ancestral effector caspases identifies novel networks for evolution of substrate specificity

Grinshpon, R. D., Shrestha, S., Titus-McQuillan, J., Hamilton, P. T., Swartz, P. D., & Clark, A. C. (2019, November 1). Biochemical Journal.

By: R. Grinshpon n, S. Shrestha *, J. Titus-McQuillan *, P. Hamilton n , P. Swartz  n & A. Clark *

MeSH headings : Animals; Caspases, Effector / chemistry; Caspases, Effector / genetics; Caspases, Effector / metabolism; Evolution, Molecular; Humans; Mammals / classification; Mammals / genetics; Phylogeny; Protein Conformation; Substrate Specificity
topics (OpenAlex): CRISPR and Genetic Engineering; Cell death mechanisms and regulation; Autophagy in Disease and Therapy
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Source: ORCID
Added: November 6, 2019

2017 article

An Internship May Not Be Enough: Enhancing Bioscience Industry Job Readiness through Practicum Experiences

Cramer, J. M., & Hamilton, P. T. (2017, April 1). Journal of Microbiology and Biology Education, Vol. 18.

By: J. Cramer n & P. Hamilton n 

topics (OpenAlex): Biomedical and Engineering Education; Genetics, Bioinformatics, and Biomedical Research
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4. Quality Education (Web of Science)
Source: ORCID
Added: January 31, 2019

2016 journal article

Phage display and structural studies reveal plasticity in substrate specificity of caspase-3a from zebrafish

Protein Science, 25(11), 2076–2088.

By: M. Tucker  n, S. MacKenzie n, J. Maciag  n, H. Dirscherl Ackerman  n, P. Swartz n, J. Yoder n , P. Hamilton n , A. Clay Clark *

Contributors: M. Tucker  n, S. MacKenzie n, J. Maciag  n, H. Ackerman  n, P. Swartz n, J. Yoder n , P. Hamilton n , A. Clark *

author keywords: phage display substrate library; substrate recognition; Danio rerio; apoptosis
MeSH headings : Allosteric Regulation; Animals; Caspase 3 / chemistry; Crystallography, X-Ray; Molecular Dynamics Simulation; Peptide Library; Substrate Specificity; Zebrafish
topics (OpenAlex): Cell death mechanisms and regulation; Autophagy in Disease and Therapy; CRISPR and Genetic Engineering
TL;DR: The structure of zebrafish Casp3a is determined to 2.28Å resolution by X‐ray crystallography, and the results suggest that a limited number of amino acid substitutions near the active site result in plasticity of the S4 sub‐site by increasing flexibility of one active site loop and by affecting hydrogen‐bonding with substrate. (via Semantic Scholar)
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Sources: ORCID, Crossref, NC State University Libraries
Added: January 31, 2019

2015 article

Exploring Protein–Protein Interactions Using Peptide Libraries Displayed on Phage

Carlson, R., Hyde-DeRuyscher, R., & Hamilton, P. (2015, February 26). Phage Display In Biotechnology and Drug Discovery, Second Edition, pp. 249–274.

By: R. Carlson, R. Hyde-DeRuyscher & P. Hamilton 

topics (OpenAlex): Monoclonal and Polyclonal Antibodies Research; Advanced Biosensing Techniques and Applications; Advanced Proteomics Techniques and Applications
Source: ORCID
Added: January 31, 2019

2015 article

Phage Display In Biotechnology and Drug Discovery

Carlson, R. O., Hyde-DeRuyscher, R., & Hamilton, P. T. (2015, February 26). Phage Display in Biotechnology and Drug Discovery, Second Edition, pp. 249–274.

By: R. Carlson, R. Hyde-DeRuyscher & P. Hamilton 

Contributors: R. Carlson, R. Hyde-DeRuyscher & P. Hamilton 

topics (OpenAlex): Monoclonal and Polyclonal Antibodies Research; Genetics, Bioinformatics, and Biomedical Research; Bacteriophages and microbial interactions
Source: ORCID
Added: February 1, 2019

2014 article

Characterization of a thermophilic bacteriophage of Geobacillus kaustophilus

Marks, T. J., & Hamilton, P. T. (2014, May 5). Archives of Virology, Vol. 159, pp. 2771–2775.

By: T. Marks  n & P. Hamilton n 

Contributors: T. Marks  n & P. Hamilton n 

MeSH headings : Bacillus subtilis / virology; Bacteriophages / genetics; Bacteriophages / isolation & purification; Base Composition; Base Sequence; DNA, Viral / genetics; Genome, Viral / genetics; Geobacillus / virology; Hot Temperature; Molecular Sequence Data; Open Reading Frames / genetics; Sequence Analysis, DNA; Soil Microbiology; Viral Proteins / genetics
topics (OpenAlex): Bacteriophages and microbial interactions; Genomics and Phylogenetic Studies; Cancer Research and Treatments
TL;DR: The overall genomic arrangement of GBK2 is similar to that of SPP1, with the majority ofGBK2SPP1-like genes coding for proteins involved in DNA replication and metabolism. (via Semantic Scholar)
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15. Life on Land (Web of Science)
Sources: Web Of Science, ORCID, NC State University Libraries
Added: August 6, 2018

2013 article

Cooperative Learning through Team-Based Projects in the Biotechnology Industry

Luginbuhl, S. C., & Hamilton, P. T. (2013, January 1). Journal of Microbiology and Biology Education, Vol. 14, pp. 221–229.

By: S. Luginbuhl n & P. Hamilton n 

topics (OpenAlex): Biomedical and Engineering Education; Problem and Project Based Learning; Engineering Education and Curriculum Development
TL;DR: Using peer-evaluations, this model of team-based, industry-sponsored projects could be implemented in other college and university courses/programs to promote professional skills and expose students to an industry setting. (via Semantic Scholar)
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4. Quality Education (Web of Science)
Source: ORCID
Added: January 31, 2019

2013 article

Improved Bone Morphogenetic Protein-2 Retention in an Injectable Collagen Matrix Using Bifunctional Peptides

Hamilton, P. T., Jansen, M. S., Ganesan, S., Benson, R. E., Hyde-DeRuyscher, R., Beyer, W. F., … al. (2013, August 8). (F. Gelain, Ed.). PLoS ONE, Vol. 8, p. e70715.

By: P. Hamilton n , M. Jansen*, S. Ganesan*, R. Benson*, R. Hyde-DeRuyscher*, W. Beyer, J. Gile, S. Nair* ...

Contributors: P. Hamilton n , M. Jansen*, S. Ganesan*, R. Benson*, R. Hyde-DeRuyscher*, W. Beyer, J. Gile, S. Nair*, J. Hodges*, H. Grøn*

Ed(s): F. Gelain

MeSH headings : Amino Acid Sequence; Animals; Biocompatible Materials / administration & dosage; Biocompatible Materials / chemistry; Biocompatible Materials / metabolism; Bone Morphogenetic Protein 2 / administration & dosage; Bone Morphogenetic Protein 2 / metabolism; Bone Morphogenetic Protein 2 / pharmacology; Collagen / administration & dosage; Collagen / chemistry; Collagen / metabolism; Injections; Male; Molecular Sequence Data; Osteogenesis / drug effects; Peptide Library; Peptides / administration & dosage; Peptides / chemistry; Peptides / metabolism; Protein Binding; Rats; Rats, Sprague-Dawley
topics (OpenAlex): Bone Tissue Engineering Materials; Silk-based biomaterials and applications; Bone and Dental Protein Studies
TL;DR: It is suggested that bifunctional peptides that can simultaneously bind to a growth factor and an implantable biomaterial can be used to control the delivery and release of growth factors at the site of implantation. (via Semantic Scholar)
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Sources: Web Of Science, ORCID, NC State University Libraries
Added: August 6, 2018

2012 journal article

Preparing Science-Trained Professionals for the Biotechnology Industry: A Ten-Year Perspective on a Professional Science Master’s Program

Journal of Microbiology & Biology Education : JMBE, 13(1), 39–44.

By: P. Hamilton n , S. Luginbuhl n & M. Hyman n 

topics (OpenAlex): Biomedical and Engineering Education
TL;DR: An overview of the PSM program in Microbial Biotechnology is provided, and some of the lessons that have been learned are shared. (via Semantic Scholar)
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UN Sustainable Development Goal Categories
4. Quality Education (Web of Science)
Sources: ORCID, Web Of Science, NC State University Libraries
Added: January 31, 2019

2009 article

Directed Assembly of PEGylated-Peptide Coatings for Infection-Resistant Titanium Metal

Khoo, X., Hamilton, P., O’Toole, G. A., Snyder, B. D., Kenan, D. J., & Grinstaff, M. W. (2009, July 21). Journal of the American Chemical Society, Vol. 131, pp. 10992–10997.

By: X. Khoo*, P. Hamilton* , G. O’Toole *, B. Snyder *, D. Kenan * & M. Grinstaff *

Contributors: X. Khoo*, P. Hamilton* , . G.A. O'Toole *, B. Snyder *, D. Kenan * & M. Grinstaff *

MeSH headings : Amino Acid Substitution; Coated Materials, Biocompatible / chemistry; Combinatorial Chemistry Techniques; Fibronectins / drug effects; Infection Control / methods; Peptide Library; Peptides / chemistry; Polyethylene Glycols / chemistry; Prostheses and Implants / microbiology; Prostheses and Implants / standards; Staphylococcus aureus / drug effects; Titanium / chemistry
topics (OpenAlex): Corrosion Behavior and Inhibition; Metal and Thin Film Mechanics; Polymer Surface Interaction Studies
TL;DR: These PEGylated-peptide coatings show promise in terms of resolving two major hurdles common to implanted metals: (i) nonspecific protein adsorption and (ii) bacterial colonization. (via Semantic Scholar)
Source: ORCID
Added: January 31, 2019

2008 conference paper

A peptide coating for the endothelialization of intravascular devices

8th World Biomaterials Congress 2008, 1, 200. http://www.scopus.com/inward/record.url?eid=2-s2.0-84869012621&partnerID=MN8TOARS

By: P. Hamilton  & A. Solan

Contributors: P. Hamilton  & A. Solan

www.scopus.com
Source: ORCID
Added: February 1, 2019

2008 conference paper

Growth factor coated sutures for improved tendon repair

8th World Biomaterials Congress 2008, 1, 179. http://www.scopus.com/inward/record.url?eid=2-s2.0-84869010182&partnerID=MN8TOARS

By: P. Hamilton , B. Buehrer & D. Juzumiene

Contributors: P. Hamilton , B. Buehrer & D. Juzumiene

www.scopus.com
Source: ORCID
Added: February 1, 2019

2007 article

Endothelialization of Titanium Surfaces

Meyers, S. R., Hamilton, P. T., Walsh, E. B., Kenan, D. J., & Grinstaff, M. W. (2007, August 2). Advanced Materials, Vol. 19, pp. 2492–2498.

By: S. Meyers *, P. Hamilton* , E. Walsh*, D. Kenan * & M. Grinstaff *

Contributors: S. Meyers *, P. Hamilton* , E. Walsh*, D. Kenan * & M. Grinstaff *

topics (OpenAlex): Diatoms and Algae Research; Polymer Surface Interaction Studies; Chemical Synthesis and Analysis
TL;DR: There is new concern regarding the use of drug-eluting stents owing to an increased thrombolytic potential of two to three fold compared to bare metal stents. (via Semantic Scholar)
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UN Sustainable Development Goal Categories
3. Good Health and Well-being (OpenAlex)
Source: ORCID
Added: January 31, 2019

2005 article

High Throughput and High Content Screening Using Peptides

Hamilton, P., Carlson, R., & Hyde-Deruyscher, R. (2005, July 27). Drug Discovery Series, pp. 347–384.

By: P. Hamilton* , R. Carlson & R. Hyde-Deruyscher

topics (OpenAlex): Cell Image Analysis Techniques; Advanced Proteomics Techniques and Applications; Advanced Biosensing Techniques and Applications
Source: ORCID
Added: January 31, 2019

2003 article

Identification and characterization of peptide probes directed against PKCα conformations

Ashraf, S. S., Anderson, E., Duke, K., Hamilton, P. T., & Fredericks, Z. (2003, May 1). Journal of Peptide Research, Vol. 61, pp. 263–273.

By: S. Ashraf, E. Anderson, K. Duke, P. Hamilton*  & Z. Fredericks*

Contributors: S. Ashraf, E. Anderson, Z. Fredericks*, K. Duke & P. Hamilton* 

author keywords: conformational probes; peptide phage display; protein kinase C; surrogate ligands
MeSH headings : Amino Acid Sequence; Binding Sites; Isoenzymes / chemistry; Isoenzymes / metabolism; Molecular Probes / chemistry; Molecular Probes / metabolism; Peptide Library; Peptides / chemistry; Peptides / metabolism; Protein Binding; Protein Kinase C / chemistry; Protein Kinase C / metabolism; Protein Kinase C-alpha
topics (OpenAlex): Monoclonal and Polyclonal Antibodies Research; Advanced Biosensing Techniques and Applications; Chemical Synthesis and Analysis
TL;DR: This work has identified separate classes of peptides that bind to protein kinase C alpha (PKCalpha) only under activation conditions, and peptide binding was specific to PKCalpha and not to any of the other closely related PKC isoforms. (via Semantic Scholar)
Source: ORCID
Added: January 31, 2019

2003 article

Intracellular Expression of Peptide Fusions for Demonstration of Protein Essentiality in Bacteria

Benson, R. E., Gottlin, E. B., Christensen, D. J., & Hamilton, P. T. (2003, August 22). Antimicrobial Agents and Chemotherapy, Vol. 47, pp. 2875–2881.

By: R. Benson*, E. Gottlin, D. Christensen & P. Hamilton* 

Contributors: R. Benson*, E. Gottlin, D. Christensen & P. Hamilton* 

MeSH headings : Bacterial Proteins / genetics; Bacterial Proteins / physiology; Blotting, Western; Culture Media; DNA Replication; DNA, Bacterial / genetics; DNA, Bacterial / metabolism; DNA, Superhelical / biosynthesis; DNA, Superhelical / genetics; Escherichia coli / growth & development; Escherichia coli / metabolism; Genetic Vectors; Peptide Library; Peptides / metabolism; Plasmids / genetics; Protein Biosynthesis; Reverse Transcriptase Polymerase Chain Reaction; Transcription, Genetic / genetics
topics (OpenAlex): RNA and protein synthesis mechanisms; Bacteriophages and microbial interactions; Bacterial Genetics and Biotechnology
TL;DR: In growth-inhibited cells, inhibition could be relieved by concurrent overexpression of the relevant target protein but not by coexpression of an irrelevant protein, indicating that growth inhibition was due to a specific interaction of the expressed peptide with its target. (via Semantic Scholar)
Source: ORCID
Added: January 31, 2019

2003 article

Surrogate Ligand-Based Assay Systems for Discovery of Antibacterial Agents for Genomic Targets

Hamilton, P., & Christensen, D. (2003, May 14). Microbial Genomics and Drug Discovery, pp. 173–185.

By: P. Hamilton*  & D. Christensen

topics (OpenAlex): Monoclonal and Polyclonal Antibodies Research; RNA and protein synthesis mechanisms; Advanced biosensing and bioanalysis techniques
Source: ORCID
Added: January 31, 2019

2001 article

Identification of Enzyme Inhibitors from Phage-Displayed Combinatorial Peptide Libraries

Kay, B. K., & Hamilton, P. T. (2001, November 1). Combinatorial Chemistry & High Throughput Screening, Vol. 4, pp. 535–543.

By: B. Kay * & P. Hamilton* 

Contributors: B. Kay * & P. Hamilton* 

MeSH headings : Amino Acid Sequence; Bacteriophages / drug effects; Bacteriophages / enzymology; Combinatorial Chemistry Techniques; Enzyme Inhibitors / pharmacology; Escherichia coli / enzymology; Ligands; Molecular Sequence Data; Peptide Library; Tetrahydrofolate Dehydrogenase / metabolism
topics (OpenAlex): Monoclonal and Polyclonal Antibodies Research; Chemical Synthesis and Analysis; Glycosylation and Glycoproteins Research
TL;DR: Data is presented on peptide ligands the authors have identified for E. coli dihydrofolate reductase (DHFR) which bind at, or near, the same site as the known inhibitors methotrexate or trimethoprim. (via Semantic Scholar)
Source: ORCID
Added: January 31, 2019

2001 article

Phage display for target-based antibacterial drug discovery

Christensen, D. J., Gottlin, E. B., Benson, R. E., & Hamilton, P. T. (2001, July 1). Drug Discovery Today, Vol. 6, pp. 721–727.

By: D. Christensen, E. Gottlin, R. Benson & P. Hamilton* 

Contributors: D. Christensen, E. Gottlin, R. Benson & P. Hamilton* 

topics (OpenAlex): Monoclonal and Polyclonal Antibodies Research; Bacteriophages and microbial interactions; RNA and protein synthesis mechanisms
TL;DR: Peptides isolated by phage display can be used as 'surrogate ligands' in competition assays for screening of targets of unknown function with small-molecule libraries, including those based on radioactive, luminescence or fluorescence detection. (via Semantic Scholar)
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3. Good Health and Well-being (OpenAlex)
Source: ORCID
Added: January 31, 2019

2000 article

Detection of small-molecule enzyme inhibitors with peptides isolated from phage-displayed combinatorial peptide libraries

Hyde-DeRuyscher, R., Paige, L. A., Christensen, D. J., Hyde-DeRuyscher, N., Lim, A., Fredericks, Z. L., … Hamilton, P. T. (2000, January 1). Chemistry & Biology, Vol. 7, pp. 17–25.

By: R. Hyde-DeRuyscher*, L. Paige, D. Christensen, N. Hyde-DeRuyscher, A. Lim*, Z. Fredericks*, J. Kranz *, P. Gallant* ...

Contributors: R. Hyde-Deruyscher*, L. Paige, D. Christensen, N. Hyde-Deruyscher, A. Lim*, Z. Fredericks*, J. Kranz *, P. Gallant* ...

author keywords: enzyme inhibitors; fluorescence polarization; high-throughput screening; peptide ligand; phage display; time-resolved fluorescence
MeSH headings : Alcohol Dehydrogenase / antagonists & inhibitors; Amino Acid Sequence; Amino Acyl-tRNA Synthetases / antagonists & inhibitors; Bacteriophages / metabolism; Biotin / chemistry; Carboxypeptidase B; Carboxypeptidases / antagonists & inhibitors; Chromatography, Affinity; Enzyme Inhibitors / analysis; Enzyme Inhibitors / isolation & purification; Enzyme Inhibitors / pharmacology; Enzyme-Linked Immunosorbent Assay; Haemophilus influenzae / enzymology; Hexokinase / antagonists & inhibitors; Kinetics; Ligands; Molecular Sequence Data; Peptide Library; Phosphorylases / antagonists & inhibitors; Tyrosine-tRNA Ligase / antagonists & inhibitors; beta-Glucosidase / antagonists & inhibitors
topics (OpenAlex): Monoclonal and Polyclonal Antibodies Research; Chemical Synthesis and Analysis; RNA and protein synthesis mechanisms
TL;DR: Peptidic surrogate ligands identified using phage display are preferentially targeted to a limited number of sites that inhibit enzyme function and can be utilized in a binding assay as a rapid and sensitive method to detect small-molecule inhibitors of target protein function. (via Semantic Scholar)
Source: ORCID
Added: January 31, 2019

1999 article

Dissection of the LXXLL Nuclear Receptor-Coactivator Interaction Motif Using Combinatorial Peptide Libraries: Discovery of Peptide Antagonists of Estrogen Receptors α and β

Chang, C.-yi, Norris, J. D., Grøn, H., Paige, L. A., Hamilton, P. T., Kenan, D. J., … McDonnell, D. P. (1999, December 1). Molecular and Cellular Biology, Vol. 19, pp. 8226–8239.

By: C. Chang *, J. Norris, H. Grøn, L. Paige, P. Hamilton* , D. Kenan *, D. Fowlkes, D. McDonnell

MeSH headings : Amino Acid Sequence; Binding Sites; Estrogen Receptor alpha; Estrogen Receptor beta; HeLa Cells; Humans; Ligands; Molecular Sequence Data; Peptide Library; Peptides / metabolism; Receptors, Cytoplasmic and Nuclear / metabolism; Receptors, Estrogen / genetics; Receptors, Estrogen / metabolism; Recombinant Fusion Proteins / genetics; Recombinant Fusion Proteins / metabolism; Transcription, Genetic; Tumor Cells, Cultured
topics (OpenAlex): Estrogen and related hormone effects; Receptor Mechanisms and Signaling; Monoclonal and Polyclonal Antibodies Research
TL;DR: It is shown that not all LXXLL motifs are functionally equivalent, a finding which suggests that it may be possible to target receptor-LXXLL interactions to develop receptor-specific antagonists. (via Semantic Scholar)
Source: ORCID
Added: January 31, 2019

1999 journal article

Dissection of the LXXLL nuclear receptor-coactivator interaction motif using combinatorial peptide libraries: Discovery of peptide antagonists of estrogen receptors α and β

Molecular and Cellular Biology, 19(12), 8226–8239. http://www.scopus.com/inward/record.url?eid=2-s2.0-0033513582&partnerID=MN8TOARS

By: C. Chang, J. Norris, H. Gr?n, L. Paige, P. Hamilton , D. Kenan, D. Fowlkes, D. McDonnell

Contributors: C. Chang, J. Norris, H. Grøn, L. Paige, P. Hamilton , D. Kenan, D. Fowlkes, D. McDonnell

www.scopus.com
Source: ORCID
Added: February 1, 2019

1999 article

Estrogen receptor (ER) modulators each induce distinct conformational changes in ER α and ER β

Paige, L. A., Christensen, D. J., Grøn, H., Norris, J. D., Gottlin, E. B., Padilla, K. M., … Fowlkes, D. M. (1999, March 30). Proceedings of the National Academy of Sciences, Vol. 96, pp. 3999–4004.

By: L. Paige*, D. Christensen*, H. Grøn*, J. Norris *, E. Gottlin *, K. Padilla*, C. Chang *, L. Ballas* ...

Contributors: L. Paige*, D. Christensen*, H. Grøn*, J. Norris *, E. Gottlin *, K. Padilla*, C. Chang *, L. Ballas* ...

MeSH headings : Amino Acid Sequence; Binding Sites; Estradiol / pharmacology; Estradiol Congeners / pharmacology; Estriol / pharmacology; Estrogen Antagonists / pharmacology; Estrogen Receptor alpha; Estrogen Receptor beta; Estrogens / pharmacology; Humans; Molecular Sequence Data; Protein Conformation / drug effects; Receptors, Estrogen / chemistry; Receptors, Estrogen / drug effects; Sequence Alignment; Sequence Homology, Amino Acid; Tamoxifen / analogs & derivatives; Tamoxifen / pharmacology
topics (OpenAlex): Estrogen and related hormone effects; Computational Drug Discovery Methods; Bioactive Compounds and Antitumor Agents
TL;DR: It is shown that ER ligands, known to produce distinct biological effects, induce distinct conformational changes in the receptors, providing a strong correlation between ER conformation and biological activity. (via Semantic Scholar)
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10. Reduced Inequalities (OpenAlex)
Source: ORCID
Added: January 31, 2019

1999 article

Peptide Antagonists of the Human Estrogen Receptor

Norris, J. D., Paige, L. A., Christensen, D. J., Chang, C.-Y., Huacani, M. R., Fan, D., … McDonnell, D. P. (1999, July 30). Science, Vol. 285, pp. 744–746.

By: J. Norris *, L. Paige, D. Christensen, C. Chang *, M. Huacani*, D. Fan*, P. Hamilton* , D. Fowlkes, D. McDonnell *

Contributors: J. Norris *, L. Paige, D. Christensen, C. Chang *, M. Huacani*, D. Fan*, P. Hamilton* , D. Fowlkes, D. McDonnell *

MeSH headings : Amino Acid Sequence; Binding Sites; Estradiol / metabolism; Estradiol / pharmacology; Estrogen Antagonists / pharmacology; Estrogen Receptor alpha; Humans; Ligands; Mifepristone / pharmacology; Molecular Sequence Data; Peptide Library; Peptides / metabolism; Peptides / pharmacology; Receptors, Cytoplasmic and Nuclear / metabolism; Receptors, Estrogen / agonists; Receptors, Estrogen / antagonists & inhibitors; Receptors, Estrogen / chemistry; Receptors, Estrogen / metabolism; Recombinant Fusion Proteins / pharmacology; Tamoxifen / metabolism; Tamoxifen / pharmacology; Transcription Factor AP-1 / genetics; Transcription Factor AP-1 / metabolism; Transcription, Genetic / drug effects
topics (OpenAlex): Estrogen and related hormone effects; Retinoids in leukemia and cellular processes; Growth Hormone and Insulin-like Growth Factors
TL;DR: The ability to regulate estrogen receptoralpha transcriptional activity by targeting sites outside of the ligand-binding pocket has implications for the development of estrogen receptor alpha antagonists for the treatment of tamoxifen-refractory breast cancers. (via Semantic Scholar)
UN Sustainable Development Goals Color Wheel
UN Sustainable Development Goal Categories
3. Good Health and Well-being (OpenAlex)
Source: ORCID
Added: February 1, 2019

1995 article

Applying Genetic Engineering to the Structural Analysis of Proteins

Hamilton, P. T. (1995, January 1). Pharmaceutical Biotechnology, pp. 329–350.

By: P. Hamilton* 

MeSH headings : Amino Acid Sequence; Base Sequence; Biotechnology; Cloning, Molecular; Coliphages / genetics; DNA, Recombinant / genetics; Escherichia coli / genetics; Gene Expression; Genetic Vectors; Molecular Sequence Data; Mutagenesis; Polymerase Chain Reaction; Protein Engineering / methods; Proteins / chemistry; Proteins / genetics; Recombinant Fusion Proteins / chemistry; Recombinant Fusion Proteins / genetics; Recombinant Fusion Proteins / isolation & purification; Technology, Pharmaceutical
topics (OpenAlex): Monoclonal and Polyclonal Antibodies Research; RNA and protein synthesis mechanisms; Glycosylation and Glycoproteins Research
TL;DR: Genetic engineering offers many techniques that can be applied to the structural analysis of proteins, which aid in the characterization of the protein but also can be applications to the generation of completely new diagnostic and therapeutic agents. (via Semantic Scholar)
Source: ORCID
Added: January 31, 2019

1995 journal article

Applying genetic engineering to the structural analysis of proteins.

Pharmaceutical Biotechnology, 7, 329–350. http://www.scopus.com/inward/record.url?eid=2-s2.0-0029449448&partnerID=MN8TOARS

By: P. Hamilton 

Contributors: P. Hamilton 

www.scopus.com
Source: ORCID
Added: February 1, 2019

1993 article

Expression of a single-chain antibody as a maltose binding protein fusion

Hamilton, P., Martinson, M., Malinowski, D., & Pearson, R. (1993, January 1). Protein Engineering Design and Selection, Vol. 6, p. 88.

By: P. Hamilton , M. Martinson, D. Malinowski & R. Pearson

Contributors: P. Hamilton , M. Martinson, D. Malinowski & R. Pearson

topics (OpenAlex): Protein purification and stability; Monoclonal and Polyclonal Antibodies Research; Viral Infectious Diseases and Gene Expression in Insects
Source: ORCID
Added: February 1, 2019

1989 article

A hydrogenase-linked gene in Methanobacterium thermoautotrophicum strain delta H encodes a polyferredoxin.

Reeve, J. N., Beckler, G. S., Cram, D. S., Hamilton, P. T., Brown, J. W., Krzycki, J. A., … Walsh, C. T. (1989, May 1). Proceedings of the National Academy of Sciences, Vol. 86, pp. 3031–3035.

By: J. Reeve *, G. Beckler*, D. Cram *, P. Hamilton* , J. Brown *, J. Krzycki*, A. Kolodziej*, L. Alex*, W. Orme-Johnson*, C. Walsh*

Contributors: J. Reeve *, G. Beckler*, D. Cram *, P. Hamilton* , J. Brown *, J. Krzycki*, A. Kolodziej*, L. Alex*, W. Orme-Johnson*, C. Walsh*

MeSH headings : Amino Acid Sequence; Base Sequence; Biological Evolution; Cloning, Molecular; Codon; Euryarchaeota / enzymology; Euryarchaeota / genetics; Ferredoxins / genetics; Genes, Bacterial; Hydrogenase / genetics; Molecular Sequence Data; Operon; Polymers; Transcription, Genetic
topics (OpenAlex): Anaerobic Digestion and Biogas Production; Microbial metabolism and enzyme function; Enzyme Structure and Function
TL;DR: The genes mvhDGA, which encode the subunit polypeptides of the methyl viologen-reducing hydrogenase in Methanobacterium thermoautotrophicum strain delta H, have been cloned and sequenced. (via Semantic Scholar)
Source: ORCID
Added: January 31, 2019

1989 article

Nucleotide sequence of the major outer membrane protein gene fromChlamydia trachomatisserovar H

Hamilton, P. T., & Malinowski, D. P. (1989, January 1). Nucleic Acids Research, Vol. 17, pp. 8366–8366.

By: P. Hamilton*  & D. Malinowski*

Contributors: P. Hamilton*  & D. Malinowski*

MeSH headings : Amino Acid Sequence; Bacterial Outer Membrane Proteins / genetics; Base Sequence; Chlamydia trachomatis / genetics; Genes, Bacterial; Molecular Sequence Data
topics (OpenAlex): Infant Nutrition and Health; Bacteriophages and microbial interactions; Legume Nitrogen Fixing Symbiosis
TL;DR: The gene encoding the major outer membrane protein (MOMP) from Chlamydia trachomatis, serovar H was isolated from a genomic library using the MOMP gene fromserovar L2 as the probe and the nucleotide sequence was determined by the dideoxy chain termination method. (via Semantic Scholar)
Source: ORCID
Added: January 31, 2019

1989 article

Polyferredoxin: A methanogen hydrogenase operon encodes a gene for a 12 × (4Fe4S) protein

Reeve, J., Beckler, G., Cram, D., Hamilton, P., Brown, J., Krzycki, J., … Walsh, C. T. (1989, August 1). Journal of Inorganic Biochemistry, Vol. 36, p. 221.

By: J. Reeve *, G. Beckler*, D. Cram *, P. Hamilton* , J. Brown *, J. Krzycki*, A. Kolodziej*, L. Alex*, W. Orme-Johnson*, C. Walsh*

topics (OpenAlex): Metalloenzymes and iron-sulfur proteins; Polyamine Metabolism and Applications; Cancer-related gene regulation
Source: ORCID
Added: January 31, 2019

1987 article

Divergence of Methanogens, Conservation of the His I Gene Sequence in all Three Biological Kingdoms and the Status of Methanobacterium Thermoautotrophicum

Reeve, J. N., Beckler, G. S., Brown, J. W., Cram, D. S., Haas, E. S., Hamilton, P. T., … Weil, C. F. (1987, January 1). Microbial Growth on C1 Compounds, pp. 255–260.

By: J. Reeve *, G. Beckler*, J. Brown *, D. Cram *, E. Haas*, P. Hamilton* , C. Morris *, B. Sherf*, C. Weil *

topics (OpenAlex): Anaerobic Digestion and Biogas Production; Microbial Metabolic Engineering and Bioproduction; Microbial metabolism and enzyme function
TL;DR: Using gene cloning in Escherichia coli to investigate the basic structure of methanogen genes and to use E. coli to synthesize meethanogen enzymes involved in methanogenesis and nitrogen fixation. (via Semantic Scholar)
Source: ORCID
Added: January 31, 2019

1986 article

Structure of methanogen genes

Reeve, J. N., Hamilton, P. T., Beckler, G. S., Morris, C. J., & Clarke, C. H. (1986, March 1). Systematic and Applied Microbiology, Vol. 7, pp. 5–12.

By: J. Reeve *, P. Hamilton* , G. Beckler*, C. Morris * & C. Clarke*

Contributors: J. Reeve *, P. Hamilton* , G. Beckler*, C. Morris * & C. Clarke*

topics (OpenAlex): Methane Hydrates and Related Phenomena; Anaerobic Digestion and Biogas Production; Microbial Community Ecology and Physiology
TL;DR: An analysis of the structure and presumed regulatory signals in sequenced methanogen genes is presented and it is indicated that a consensus core ribosome binding sequence for methanogens would be 5 'AGGTGA and that many of the ribosom binding sequences are, in fact, longer than the core sequence containing 7 bases with the potential to hybridize to 16SrRNA. (via Semantic Scholar)
Source: ORCID
Added: January 31, 2019

1985 article

Sequence divergence of an archaebacterial gene cloned from a mesophilic and a thermophilic methanogen

Hamilton, P. T., & Reeve, J. N. (1985, December 1). Journal of Molecular Evolution, Vol. 22, pp. 351–360.

By: P. Hamilton*  & J. Reeve *

Contributors: P. Hamilton*  & J. Reeve *

MeSH headings : Amino Acid Sequence; Base Composition; Base Sequence; Biological Evolution; Cloning, Molecular; Escherichia coli / genetics; Euryarchaeota / genetics; Genes; Genes, Bacterial; Genetic Variation; Genotype; Phenotype; Plasmids; Species Specificity
topics (OpenAlex): Photoreceptor and optogenetics research; Photosynthetic Processes and Mechanisms; Enzyme Structure and Function
TL;DR: A 1.6-kb fragment of DNA from the thermophilic, methane-producing, anaerobic archaebacteriumMethanobacterium thermoautotrophicum ΔH has been cloned and sequenced and predicts that complementation in E. coli results from the synthesis of a polypeptide with a molecular weight of 36,249. (via Semantic Scholar)
Source: ORCID
Added: January 31, 2019

1985 article

Structure of genes and an insertion element in the methane producing archaebacterium Methanobrevibacter smithii

Hamilton, P. T., & Reeve, J. N. (1985, June 1). Molecular and General Genetics MGG, Vol. 200, pp. 47–59.

By: P. Hamilton*  & J. Reeve *

Contributors: P. Hamilton*  & J. Reeve *

MeSH headings : Amino Acid Sequence; Archaea / genetics; Bacteria / genetics; Bacterial Proteins / genetics; Base Sequence; Cloning, Molecular; Codon; DNA Restriction Enzymes; DNA Transposable Elements; Escherichia coli / genetics; Genes; Genes, Bacterial; Genotype; Methane / metabolism; Mutation; Nucleic Acid Hybridization; Phenotype
topics (OpenAlex): Biofuel production and bioconversion; Genomics and Phylogenetic Studies; Microbial bioremediation and biosurfactants
TL;DR: Sequence analyses, transposon mutagenesis and expression in E. coli minicells indicate that purE and proC complementations result from the synthesis of M. smithii polypeptides, and comparison of data with sequences from halophilic archaebacteria suggests that 5′GAANTTTCA and 5′TTTTAATATAAA may be consensus promoter sequences for archaEBacteria. (via Semantic Scholar)
Source: ORCID
Added: January 31, 2019

1982 journal article

Beginning genetics with methanogens.

Basic Life Sciences, 19, 233–244. http://www.scopus.com/inward/record.url?eid=2-s2.0-0020009847&partnerID=MN8TOARS

By: J. Reeve, N. Trun & P. Hamilton 

Contributors: J. Reeve, N. Trun & P. Hamilton 

www.scopus.com
Source: ORCID
Added: February 1, 2019

Employment

Updated: July 5th, 2016 10:20

2009 - present

North Carolina State University Raleigh, NC, US
Associate Professor Plant and Microbial Biology

Education

Updated: July 5th, 2016 10:27

1980 - 1984

Ohio State University Columbus, OH, US
Ph.D in Microbiology Microbiology

1976 - 1980

University of Michigan Ann Arbor, MI, US
Bachelor of Science in Microbiology

Funding History

Funding history based on the linked ORCID record. Updated: December 10th, 2019 19:37

grant September 1, 2019 - August 1, 2022
NSF IGE: Accelerate to Industry (A2i)
National Science Foundation
grant September 15, 2015 - May 31, 2016
I-Corps: Industrial Enzymes from the Microbiomes of Household Insects
National Science Foundation - Directorate for Engineering
grant May 15, 2008 - April 30, 2009
Antibiotic-binding Peptides for Biofilm Prevention on Ventriculoperitoneal Shunts
National Institute of Neurological Disorders and Stroke
grant May 15, 2008 - April 30, 2009
Antibiotic-binding Peptides for Biofilm Prevention on Ventriculoperitoneal Shunts
National Institute of Neurological Disorders and Stroke
grant April 14, 2008 - August 1, 2009
Localized Growth Factor Therapy for Surgical Hernia Repair
National Institute of General Medical Sciences
grant April 14, 2008 - August 1, 2009
Localized Growth Factor Therapy for Surgical Hernia Repair
National Institute of General Medical Sciences
grant September 20, 2007 - August 31, 2010
Interfacial Adapters for Improved Cell Delivery to Tissues
National Heart Lung and Blood Institute
grant July 2, 2007 - June 30, 2008
Point of Care Attachment of Multiple Antibiotics onto Metal Implants
National Institute of Dental and Craniofacial Research
grant July 2, 2007 - July 31, 2011
Point of Care Attachment of Antibiotics onto Metal Implants
National Institute of Allergy and Infectious Diseases
grant July 2, 2007 - June 30, 2008
Point of Care Attachment of Multiple Antibiotics onto Metal Implants
National Institute of Dental and Craniofacial Research
grant September 30, 2006 - September 29, 2008
Discovery of a Platelet Derived Growth Factor Peptide-based Mimetic
National Institute of Diabetes and Digestive and Kidney Diseases
grant September 30, 2006 - September 29, 2008
Discovery of a Platelet Derived Growth Factor Peptide-based Mimetic
National Institute of Diabetes and Digestive and Kidney Diseases
grant September 1, 2006 - August 31, 2007
Injectable BMP-2 Formulation for Fracture Healing
National Institute of Arthritis and Musculoskeletal and Skin Diseases
grant September 1, 2006 - August 31, 2007
Injectable BMP-2 Formulation for Fracture Healing
National Institute of Arthritis and Musculoskeletal and Skin Diseases
grant June 28, 2006 - March 31, 2008
Growth Factor Coated Sutures for Improved Tendons Repair
National Institute of Arthritis and Musculoskeletal and Skin Diseases
grant June 28, 2006 - March 31, 2008
Growth Factor Coated Sutures for Improved Tendons Repair
National Institute of Arthritis and Musculoskeletal and Skin Diseases
grant July 1, 2005 - June 30, 2007
Discovery of Peptide-based Bone Morphogenic Protein Mimetics
National Institute of Arthritis and Musculoskeletal and Skin Diseases
grant July 1, 2005 - June 30, 2007
Discovery of Peptide-based Bone Morphogenic Protein Mimetics
National Institute of Arthritis and Musculoskeletal and Skin Diseases
grant April 1, 2005 - March 31, 2006
Improved Orthopedic Implant Surface Coatings
National Institute of Arthritis and Musculoskeletal and Skin Diseases
grant April 1, 2005 - March 31, 2006
Improved Orthopedic Implant Surface Coatings
National Institute of Arthritis and Musculoskeletal and Skin Diseases
grant April 1, 2004 - August 31, 2009
Improved Orthopedic Implant Surface Coatings
National Institute of Arthritis and Musculoskeletal and Skin Diseases
grant April 1, 2004 - August 31, 2009
Improved Orthopedic Implant Surface Coatings
National Institute of Arthritis and Musculoskeletal and Skin Diseases
grant September 1, 1999 - February 28, 2001
A NOVEL APPROACH TO THE DISCOVERY OF ANTIBACTERIAL DRUGS
National Institute of Allergy and Infectious Diseases
grant September 1, 1999 - February 28, 2001
A NOVEL APPROACH TO THE DISCOVERY OF ANTIBACTERIAL DRUGS
National Institute of Allergy and Infectious Diseases

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