Pei-Shan Wu

Works (3)

Updated: September 12th, 2023 05:02

2023 journal article

An Optimized Small-Scale Rearing System to Support Embryonic Microinjection Protocols for Western Corn Rootworm, Diabrotica virgifera virgifera

Insects, 14(8), 683.

By: F. Chu n, P. Wu n, S. Pinzi n, N. Grubbs n, A. Cohen n  & M. Lorenzen n 

author keywords: insect rearing; rootworm; molecular biology; function genomics; CRISPR
TL;DR: An optimized rearing system for use in western corn rootworm functional genomics research is reported, specifically the development of a system that facilitates the collection of preblastoderm embryos for microinjection as well as gathering large larvae and pupae for downstream phenotypic screening. (via Semantic Scholar)
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2. Zero Hunger (Web of Science)
Sources: Web Of Science, NC State University Libraries, ORCID, Crossref
Added: September 11, 2023

2018 journal article

Microinjection of Western Corn Rootworm, <em>Diabrotica virgifera virgifera</em>, Embryos for Germline Transformation, or CRISPR/Cas9 Genome Editing

Journal of Visualized Experiments, (134).

By: F. Chu n, P. Wu n, S. Pinzi n, N. Grubbs n & M. Lorenzen n 

author keywords: Bioengineering; Issue 134; embryonic microinjection; functional genomics; germline transformation; transgenesis; CRISPR/Cas9 genome editing; western corn rootworm
MeSH headings : Animals; CRISPR-Cas Systems / genetics; Coleoptera; Gene Editing / methods; Germ-Line Mutation / genetics; Microinjections / methods; RNA Interference
TL;DR: The methods used to collect and microinject precellular WCR embryos with nucleic acids are detailed, aimed at the creation of transgenic WCR, and CRISPR/Cas9-genome editing could also be performed using the same protocols, with the only difference being the composition of the solution injected into the embryos. (via Semantic Scholar)
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Sources: Web Of Science, NC State University Libraries, Crossref
Added: October 16, 2018

2017 journal article

Germline transformation of the western corn rootworm, Diabrotica virgifera virgifera

Insect Molecular Biology, 26(4), 440–452.

By: F. Chu n, W. Klobasa n, P. Wu n, S. Pinzi n, N. Grubbs n, S. Gorski  n, Y. Cardoza n, M. Lorenzen n 

author keywords: Minos; piggyBac; small-scale rearing; embryonic microinjection
MeSH headings : Animals; Coleoptera / genetics; Female; Gene Transfer Techniques; Luminescent Proteins; Male; Transposases
TL;DR: Establishing transgenic technologies for this beetle is the first step towards bringing a wide range of transformation‐based tools to bear on understanding WCR biology. (via Semantic Scholar)
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Sources: Web Of Science, NC State University Libraries, Crossref
Added: August 6, 2018

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