Pei-Shan Wu

Works (3)

Updated: September 12th, 2023 05:02

2023 journal article

An Optimized Small-Scale Rearing System to Support Embryonic Microinjection Protocols for Western Corn Rootworm, Diabrotica virgifera virgifera

Insects, 14(8), 683.

By: F. Chu n, P. Wu n, S. Pinzi n, N. Grubbs n, A. Cohen n  & M. Lorenzen n 

author keywords: insect rearing; rootworm; molecular biology; function genomics; CRISPR
topics (OpenAlex): Insect Resistance and Genetics; CRISPR and Genetic Engineering; Viral Infectious Diseases and Gene Expression in Insects
TL;DR: An optimized rearing system for use in western corn rootworm functional genomics research is reported, specifically the development of a system that facilitates the collection of preblastoderm embryos for microinjection as well as gathering large larvae and pupae for downstream phenotypic screening. (via Semantic Scholar)
UN Sustainable Development Goals Color Wheel
UN Sustainable Development Goal Categories
2. Zero Hunger (Web of Science)
Sources: Web Of Science, NC State University Libraries, ORCID, Crossref
Added: September 11, 2023

2018 journal article

Microinjection of Western Corn Rootworm, <em>Diabrotica virgifera virgifera</em>, Embryos for Germline Transformation, or CRISPR/Cas9 Genome Editing

Journal of Visualized Experiments, (134).

By: F. Chu n, P. Wu n, S. Pinzi n, N. Grubbs n & M. Lorenzen n 

author keywords: Bioengineering; Issue 134; embryonic microinjection; functional genomics; germline transformation; transgenesis; CRISPR/Cas9 genome editing; western corn rootworm
MeSH headings : Animals; CRISPR-Cas Systems / genetics; Coleoptera; Gene Editing / methods; Germ-Line Mutation / genetics; Microinjections / methods; RNA Interference
topics (OpenAlex): Insect Resistance and Genetics; CRISPR and Genetic Engineering; Viral Infectious Diseases and Gene Expression in Insects
TL;DR: The methods used to collect and microinject precellular WCR embryos with nucleic acids are detailed, aimed at the creation of transgenic WCR, and CRISPR/Cas9-genome editing could also be performed using the same protocols, with the only difference being the composition of the solution injected into the embryos. (via Semantic Scholar)
UN Sustainable Development Goals Color Wheel
UN Sustainable Development Goal Categories
Sources: Web Of Science, NC State University Libraries, Crossref
Added: October 16, 2018

2017 journal article

Germline transformation of the western corn rootworm, Diabrotica virgifera virgifera

Insect Molecular Biology, 26(4), 440–452.

By: F. Chu n, W. Klobasa n, P. Wu n, S. Pinzi n, N. Grubbs n, S. Gorski  n, Y. Cardoza n, M. Lorenzen n 

author keywords: Minos; piggyBac; small-scale rearing; embryonic microinjection
MeSH headings : Animals; Coleoptera / genetics; Female; Gene Transfer Techniques; Luminescent Proteins; Male; Transposases
topics (OpenAlex): Insect Resistance and Genetics; CRISPR and Genetic Engineering; Animal Genetics and Reproduction
TL;DR: Establishing transgenic technologies for this beetle is the first step towards bringing a wide range of transformation‐based tools to bear on understanding WCR biology. (via Semantic Scholar)
UN Sustainable Development Goals Color Wheel
UN Sustainable Development Goal Categories
Sources: Web Of Science, NC State University Libraries, Crossref
Added: August 6, 2018

Citation Index includes data from a number of different sources. If you have questions about the sources of data in the Citation Index or need a set of data which is free to re-distribute, please contact us.

Certain data included herein are derived from the Web of Science© and InCites© (2025) of Clarivate Analytics. All rights reserved. You may not copy or re-distribute this material in whole or in part without the prior written consent of Clarivate Analytics.