@article{jia_read_2023, title={Bacteria Disinfection of Rice Seeds by Ultraviolet Light Irradiation in a Biosafe Flow Cabinet}, ISSN={["1535-1025"]}, DOI={10.1094/PHP-02-23-0017-RS}, abstractNote={ Easy-to-use methods to disinfect microbes on seeds are not available. In the present study, rice seeds were irradiated by 274-nm germicidal ultraviolet (UV) protons in a biosafe flow cabinet. Seeds without any barrier, in a sterilized mesh bag, or in a sterilized coin envelope were removed each day with sterilized forceps for 7 days after UV irradiation and placed in nutrient agar media in a dark incubator at 29°C for 3 days, after which the number of seeds contaminated with bacteria and/or fungi were counted. At the same time, 10 seeds each time were removed from each UV treatment and kept in a dark incubator at 40°C for 5 days to determine the germination rate. Both bacterial and fungal infection rates declined significantly over time. The germination rate, with an average of 90 ± 7.1%, did not change significantly over time. There was no significant difference among treatments for germination rate or fungal infection rate. The UV irradiation of seeds in mesh bags had the strongest effect on reducing bacterial infection rates over time, whereas the direct UV irradiation had a weaker effect on bacterial infection rates than the UV irradiation of seeds in mesh bags. We suggest that UV irradiation of seeds in mesh bags be used to reduce seed bacterial contamination. }, journal={PLANT HEALTH PROGRESS}, author={Jia, Yulin and Read, Quentin D.}, year={2023}, month={May} } @article{mengistu_kelly_read_ray_bellaloui_schumacher_2023, title={Charcoal Rot Severity and Soybean Yield Responses to Planting Date, Irrigation, and Genotypes}, ISSN={["1943-7692"]}, DOI={10.1094/PDIS-06-22-1329-RE}, abstractNote={ Soybean (Glycine max [L.] Merr.) production is influenced by planting date, but its impact on yield in fields infested with Macrophomina phaseolina (Tassi) Goid. is unknown. A 3-year study was conducted in M. phaseolina-infested fields to assess the effects of planting date (PD) on disease severity and yield using eight genotypes, four of which are reported to be susceptible to charcoal rot (S), and four reported with moderate resistance (MR) to charcoal rot (CR). The genotypes were planted in early April, early May, and early June under irrigated and nonirrigated conditions. There was planting date by irrigation interaction for area under the disease progress curve (AUDPC) where May PD was significantly lower compared to April and June PDs in irrigated environments but not in nonirrigated environments. Correspondingly, yield in April PD was significantly lower than that of May and June. Interestingly, yield of S genotypes increased significantly with each subsequent PD, while yield of MR genotypes remained high across all three PDs. The interaction of genotypes by PD on yield revealed that the MR genotypes DT97-4290 and DS-880 had the greatest yields in May compared to April. While May PD had a decreased AUDPC and an increased yield across genotypes, the result of this research suggests that in fields infested with M. phaseolina, early May to early June planting coupled with appropriate cultivar selection provides maximum yield potential for western Tennessee and mid-southern soybean growers. }, journal={PLANT DISEASE}, author={Mengistu, Alemu and Kelly, Heather M. M. and Read, Quentin D. D. and Ray, Jeffrey D. D. and Bellaloui, Nacer and Schumacher, Lesley A. A.}, year={2023}, month={Feb} } @article{ewert_simone-finstrom_read_husseneder_ricigliano_2023, title={Effects of ingested essential oils and propolis extracts on honey bee (Hymenoptera: Apidae) health and gut microbiota}, volume={23}, ISSN={["1536-2442"]}, DOI={10.1093/jisesa/iead087}, abstractNote={Abstract}, number={6}, journal={JOURNAL OF INSECT SCIENCE}, author={Ewert, Allyson Martin and Simone-Finstrom, Michael and Read, Quentin and Husseneder, Claudia and Ricigliano, Vincent}, year={2023}, month={Nov} } @article{gurung_rosa_yelvington_terry_read_piccolo_moody_tripp_pittman_fay_et al._2023, title={Evaluation of a Plant-Based Infant Formula Containing Almonds and Buckwheat on Gut Microbiota Composition, Intestine Morphology, Metabolic and Immune Markers in a Neonatal Piglet Model}, volume={15}, ISSN={["2072-6643"]}, DOI={10.3390/nu15020383}, abstractNote={A controlled-neonatal piglet trial was conducted to evaluate the impact of a plant-based infant formula containing buckwheat and almonds as the main source of protein compared to a commercially available dairy-based formula on the gut health parameters. Two day old piglets were fed either a plant-based or a dairy-based formula until day 21. Gut microbiome, cytokines, growth and metabolism related outcomes, and intestinal morphology were evaluated to determine the safety of the plant-based infant formula. This study reported that the plant-based formula-fed piglets had a similar intestinal microbiota composition relative to the dairy-based formula-fed group. However, differential abundance of specific microbiota species was detected within each diet group in the small and large intestinal regions and fecal samples. Lactobacillus delbrueckii, Lactobacillus crispatus, and Fusobacterium sp. had higher abundance in the small intestine of plant-based formula-fed piglets compared to the dairy-based group. Bacteroides nordii, Enterococcus sp., Lactobacillus crispatus, Prevotella sp., Ruminococcus lactaris, Bacteroides nordii, Eisenbergiella sp., Lactobacillus crispatus, Prevotella sp., and Akkermansia muciniphila had greater abundance in the large intestine of the plant based diet fed piglets relative to the dairy-based diet group. In the feces, Clostridiales, Bacteroides uniformis, Butyricimonasvirosa, Cloacibacillus porcorum, Clostridium clostridioforme, and Fusobacterium sp. were abundant in dairy-based group relative to the plant-based group. Lachnospiraceae, Clostridium scindens, Lactobacillus coleohominis, and Prevetolla sp. had greater abundance in the feces of the plant-based group in comparison to the dairy-based group. Gut morphology was similar between the plant and the dairy-based formula-fed piglets. Circulatory cytokines, magnesium, triiodothyronine (T3), thyroxine (T4), thyroid stimulating hormone (TSH), vitamin D, vitamin K, and IgE levels were similar among all piglets independent of dietary group. Overall, the present study demonstrated that a plant-based formula with buckwheat and almonds as the primary source of protein can support similar gut microbiota growth and health outcomes compared to a dairy-based infant formula.}, number={2}, journal={NUTRIENTS}, author={Gurung, Manoj and Rosa, Fernanda and Yelvington, Brooke and Terry, Nathan and Read, Quentin D. and Piccolo, Brian D. and Moody, Becky and Tripp, Patricia and Pittman, Hoy E. and Fay, Bobby L. and et al.}, year={2023}, month={Jan} } @article{allan_read_johanningsmeier_2023, title={Impact of sweetpotato starch structures, thermal properties, and granules sizes on sweetpotato fry textures}, volume={137}, ISSN={["1873-7137"]}, DOI={10.1016/j.foodhyd.2022.108377}, abstractNote={Sweetpotato French fry (SPFF) textures have been previously correlated with starch contents, but little is known about the relationship of starch properties with SPFF textures. Associations of SPFF textures with starch properties were explored by comparing starch thermal properties (gelatinization temperatures and starch type percentages), amylopectin chain length distributions, and granules sizes among genotypes that produced a wide range of SPFF textures. SPFF textures were modeled with these starch data and raw sweetpotato compositions to investigate the impact of starch attributes on SPFF textures. Starch structures, thermal properties, and granule sizes varied among genotypes. In general, starches from orange-fleshed sweetpotatoes had lower degrees of polymerization (DP) (more DP 6–9 chains and fewer DP 18–25 chains), more B-type starch, and lower gelatinization temperatures than yellow or cream-fleshed genotypes, but no differences in granule size distributions. Predictions of fry textures using raw composition were significantly improved when starch properties were included. Starch thermal properties, particularly the percentage of A-type starch, most influenced the prediction of SPFF textures. Structural equation modeling identified causal relationships between SPFF textures and starch properties. Starch thermal properties and the interaction with α-amylase levels had the strongest correlations with SPFF textures. Alongside raw compositions, starch thermal properties provide valuable information for predicting SPFF textures that could be used to make varietal selections by processors and breeders.}, journal={FOOD HYDROCOLLOIDS}, author={Allan, Matthew C. and Read, Quentin D. and Johanningsmeier, Suzanne D.}, year={2023}, month={Apr} } @article{allan_read_johanningsmeier_2023, title={Impact of sweetpotato starch structures, thermal properties, and granules sizes on sweetpotato fry textures (vol 137, 108377, 2023)}, volume={139}, ISSN={["1873-7137"]}, DOI={10.1016/j.foodhyd.2023.108559}, journal={FOOD HYDROCOLLOIDS}, author={Allan, Matthew C. and Read, Quentin D. and Johanningsmeier, Suzanne D.}, year={2023}, month={May} } @article{cowger_read_clark_dong_2023, title={Optimal Timing of Fungicide Application to Manage Fusarium Head Blight in Winter Barley}, volume={4}, ISSN={["1943-7692"]}, url={https://doi.org/10.1094/PDIS-01-23-0021-RE}, DOI={10.1094/PDIS-01-23-0021-RE}, abstractNote={ Fusarium head blight (FHB) is among the chief threats to profitable barley production, and fungicide applications are one of two main strategies for reducing FHB damage to barley crops. However, there is very little published information on optimal timing of such applications. A 4-year field study was conducted with winter barley in Raleigh, North Carolina, to compare three timings for fungicide application: 50% spike emergence (Zadoks growth stage or GS 55), 100% spike emergence (GS 59), and 6 days after GS 59. Three winter barley cultivars with varying levels of FHB resistance were grown for four successive years (2018 to 2021) in a split-plot experiment and inoculated each spring with Fusarium-infected corn spawn. Three fungicides were compared: propiconazole + pydiflumetofen (Miravis Ace), prothioconazole + tebuconazole (Prosaro), and metconazole (Caramba). Correlations among visual symptoms and assays of harvested grain were modest and were weakened by fungicide applications. Across years and cultivars, deoxynivalenol (DON) and percent Fusarium-infected kernels were most reduced relative to the nontreated control by fungicide applications at the latest timing (GS 59 + 6 days). The early (GS 55) timing resulted in DON not significantly different from the nontreated control. Based on these results, it is recommended that to minimize damage from FHB, fungicide should be applied to winter barley several days after GS 59 (100% spike emergence), and not before GS 59. }, journal={PLANT DISEASE}, author={Cowger, Christina and Read, Quentin D. D. and Clark, Logan and Dong, Yanhong}, year={2023}, month={Apr} } @article{taliercio_eickholt_read_carter_waldeck_fallen_2023, title={Parental choice and seed size impact the uprightness of progeny from interspecific Glycine hybridizations}, ISSN={["1435-0653"]}, DOI={10.1002/csc2.21015}, abstractNote={Abstract}, journal={CROP SCIENCE}, author={Taliercio, Earl and Eickholt, David and Read, Quentin D. and Carter, Thomas and Waldeck, Nathan and Fallen, Ben}, year={2023}, month={Jun} } @article{kandel_sandoya_zhou_read_mou_simko_2022, title={Identification of Quantitative Trait Loci Associated with Bacterial Leaf Spot Resistance in Baby Leaf Lettuce}, ISSN={["1943-7692"]}, DOI={10.1094/PDIS-09-21-2087-RE}, abstractNote={ Spring mix is a popular packaged salad that contains lettuce (Lactuca sativa L.) as one of its main ingredients. Plants for baby leaf lettuce (BLL) production are grown at very high densities, which enhances the occurrence of bacterial leaf spot (BLS) caused by Xanthomonas hortorum pv. vitians (Xhv), a disease that can make the crop unmarketable. The market demands disease-free, high-quality BLL all year round. Growing highly BLS-resistant cultivars will reduce loss of yield and quality, thus minimizing economic detriment to lettuce and spring mix growers. The research objectives were to identify lettuce accessions resistant to BLS and associated quantitative trait loci (QTL). A total of 495 lettuce accessions were screened with six isolates (BS0347, BS2861, BS3127, L7, L44, and Sc8B) of Xhv. Accessions showing overall high-level resistance to all tested Xhv isolates were ‘Bunte Forellen’, PI 226514, ‘La Brillante’, ARM09-161-10-1-4, ‘Grenadier’, ‘Bella’, PI 491210, ‘Delight’, and ‘Romana Verde del Mercado’. Genome-wide association studies of BLS resistance by mixed linear model analyses identified significant QTLs on four lettuce chromosomes (2, 4, 6, and 8). The most significant QTL was on Chromosome 8 (P = 1.42 × 10−7), which explained 6.7% of total phenotypic variation for the disease severity. Accessions with a high level of resistance detected in this study are valuable resources for lettuce germplasm improvement. Molecular markers closely linked to QTLs can be considered for marker-assisted selection to develop new BLL lettuce cultivars with resistance to multiple races of Xhv. }, journal={PLANT DISEASE}, author={Kandel, Jinita Sthapit and Sandoya, German V and Zhou, Wei and Read, Quentin D. and Mou, Beiquan and Simko, Ivan}, year={2022}, month={Sep} }