Qingbo Shu

Works (18)

Updated: December 9th, 2024 08:35

2024 journal article

The biochemical effects of carotenoids in orange carrots on the colonic proteome in a mouse model of diet-induced obesity

FRONTIERS IN NUTRITION, 11.

By: E. Balbuena n, F. Milhem n, B. Kiremitci n, T. Williams n , L. Collins n , Q. Shu n , A. Eroglu n 

Contributors: E. Balbuena n, F. Milhem n, B. Kiremitci n, T. Williams n , L. Collins n , Q. Shu n , A. Eroglu n 

author keywords: carotenoids; quantitative proteomics; colon; intestinal barrier; diet-induced obesity
topics (OpenAlex): Antioxidant Activity and Oxidative Stress; Cholesterol and Lipid Metabolism; Adipokines, Inflammation, and Metabolic Diseases
Sources: Web Of Science, NC State University Libraries, ORCID
Added: December 2, 2024

2023 journal article

Cerebrospinal Fluid Protein Markers Indicate Neuro-Damage in SARS-CoV-2-Infected Nonhuman Primates

Molecular & Cellular Proteomics.

By: S. Maity *, M. Mayer, Q. Shu* , H. Linh, D. Bao *, R. Blair, Y. He *, C. Lyon* ...

MeSH headings : Animals; Humans; Chlorocebus aethiops; SARS-CoV-2; COVID-19; Cerebrospinal Fluid Proteins; Proteome; Macaca mulatta
topics (OpenAlex): Long-Term Effects of COVID-19; Neuroinflammation and Neurodegeneration Mechanisms; COVID-19 Clinical Research Studies
TL;DR: The results indicate that CSF proteome changes after infection resolution correspond with bronchial virus abundance during early infection and revealed substantial differences between the infected NHPs and their age-matched uninfected controls, suggesting these differences could reflect altered secretion of CNS factors in response to SARS-CoV-2-induced neuropathology. (via Semantic Scholar)
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3. Good Health and Well-being (OpenAlex)
Source: ORCID
Added: May 2, 2023

2022 journal article

Assay design for unambiguous identification and quantification of circulating pathogen-derived peptide biomarkers

Theranostics.

Contributors: Q. Shu* , S. Liu *, T. Alonzi *, S. LaCourse *, D. Singh *, D. Bao *, D. Wamalwa *, L. Jiang * ...

MeSH headings : Animals; Biomarkers; Mycobacterium tuberculosis; Peptides; Sensitivity and Specificity; Tuberculosis / diagnosis; Tuberculosis / microbiology
topics (OpenAlex): Tuberculosis Research and Epidemiology; Mycobacterium research and diagnosis; Antibiotic Resistance in Bacteria
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3. Good Health and Well-being (OpenAlex)
Source: ORCID
Added: May 2, 2023

2022 journal article

MS-based Detection of Pathogen Antigen Through Immunoprecipitation of Its Proteolytic Peptides

Molecular & Cellular Proteomics.

Contributors: Q. Shu , J. Fan, C. Lyon & T. Hu

topics (OpenAlex): Enterobacteriaceae and Cronobacter Research; Biosensors and Analytical Detection
Source: ORCID
Added: May 2, 2023

2022 journal article

Nanopore-based disease diagnosis using pathogen-derived tryptic peptides from serum

Nano Today.

Contributors: W. Zheng *, J. Saliba*, X. Wei *, Q. Shu* , L. Pierson *, L. Mao*, C. Liu *, C. Lyon * ...

topics (OpenAlex): Nanopore and Nanochannel Transport Studies; Microfluidic and Bio-sensing Technologies; Caveolin-1 and cellular processes
TL;DR: It is demonstrated that a peptide identified by this approach produced high-affinity antibodies and yielded a characteristic peptide signature that was detectable over a broad linear range, to detect and quantify a pathogen-derived peptide from digested human serum samples with high sensitivity and specificity. (via Semantic Scholar)
Source: ORCID
Added: May 2, 2023

2022 journal article

Peptidomic analysis of mycobacterial secreted proteins enables species identification

View.

Contributors: Q. Shu* , M. Rajagopal*, J. Fan*, L. Zhan *, X. Kong*, Y. He *, S. Rotcheewaphan *, C. Lyon * ...

topics (OpenAlex): Mycobacterium research and diagnosis; Tuberculosis Research and Epidemiology; Antibiotic Resistance in Bacteria
TL;DR: A novel immunoprecipitation‐coupled mass spectrometry assay that can distinguish Esx proteins that are expressed by slow‐growing mycobacterial species commonly detected in clinical isolates is described. (via Semantic Scholar)
Source: ORCID
Added: May 2, 2023

2021 journal article

Species-specific quantification of circulating ebolavirus burden using VP40-derived peptide variants

PLOS Pathogens, 17(11), e1010039.

By: Q. Shu* , T. Kenny*, J. Fan *, C. Lyon *, L. Cazares * & T. Hu *

Contributors: Q. Shu* , T. Kenny*, J. Fan *, C. Lyon *, L. Cazares * & T. Hu *

Ed(s): A. Hartman

MeSH headings : Animals; Ebolavirus / immunology; Hemorrhagic Fever, Ebola / blood; Hemorrhagic Fever, Ebola / diagnosis; Hemorrhagic Fever, Ebola / immunology; Hemorrhagic Fever, Ebola / virology; Humans; Macaca mulatta; Peptide Fragments / immunology; Recombinant Proteins / immunology; Species Specificity; Viral Matrix Proteins / immunology
topics (OpenAlex): Viral Infections and Outbreaks Research; Viral Infections and Vectors; Virology and Viral Diseases
TL;DR: An immunoprecipitation-coupled mass spectrometry (IP-MS) assay for VP40 antigen detection and quantification that differentiates four of the six known EBV species in one assay, through a combination of p8 and p12 data. (via Semantic Scholar)
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15. Life on Land (OpenAlex)
Source: ORCID
Added: May 2, 2023

2020 journal article

Destabilization of exotoxin A diminishes serum antibody titer and affinity maturation by reducing peptide-MHCII abundance

The Journal of Immunology. https://publons.com/wos-op/publon/57950976/

Contributors: D. Moss, K. Song, A. Wanek, Q. Shu , T. Hu, J. Kolls, R. Mettu, S. Landry

publons.com
Source: ORCID
Added: May 2, 2023

2020 article

IP-MS Analysis of ESX-5 and ESX-1 Substrates Enables Mycobacterial Species Identification

Shu, Q., Rajagopal, M., Fan, J., Zhan, L., Kong, X., He, Y., … Hu, T. (2020, June 7).

By: Q. Shu* , M. Rajagopal*, J. Fan*, L. Zhan *, X. Kong*, Y. He *, S. Rotcheewaphan *, C. Lyon * ...

topics (OpenAlex): Mycobacterium research and diagnosis; Tuberculosis Research and Epidemiology; Infectious Diseases and Mycology
TL;DR: This IP-MS assay employs antibodies specific for conserved tryptic peptides of M. tuberculosis EsxN (AQAASLEAEHQAIVR) and CFP-10 (TQIDQVESTAGSLQGQWR) to capture and identify specific mycobacterial species and allows species-specific myCobacterium identification at the first sign of MGIT culture positivity. (via Semantic Scholar)
Source: ORCID
Added: May 2, 2023

2020 journal article

Large-scale Identification of N-linked Intact Glycopeptides in Human Serum using HILIC Enrichment and Spectral Library Search

Molecular & Cellular Proteomics, 19(4), 672–689.

By: Q. Shu* , M. Li *, L. Shu *, Z. An *, J. Wang *, H. Lv *, M. Yang *, T. Cai * ...

Contributors: Q. Shu* , M. Li *, L. Shu *, Z. An *, J. Wang *, H. Lv *, M. Yang *, T. Cai * ...

MeSH headings : Amino Acid Sequence; Biomarkers / blood; Blood Coagulation; Blood Proteins / analysis; Blood Proteins / chemistry; Cell Adhesion Molecules / blood; Cell Lineage; Complement System Proteins / metabolism; Databases, Protein; Glycopeptides / blood; Glycopeptides / chemistry; Glycoproteins / blood; Glycoproteins / chemistry; Glycosylation; Humans; Hydrophobic and Hydrophilic Interactions; Molecular Weight; Peptide Library; Polysaccharides / chemistry; Reference Standards; Reproducibility of Results; Software
topics (OpenAlex): Glycosylation and Glycoproteins Research; Machine Learning in Bioinformatics; Genomics and Phylogenetic Studies
TL;DR: Large-scale identification of N-linked intact glycopeptides by liquid chromatography coupled with tandem mass spectrometry in human serum is challenging because of the wide dynamic range of serum protein abundances, the lack of a complete serum N-glycan database and the existence of proteoforms. (via Semantic Scholar)
Source: ORCID
Added: May 2, 2023

2019 journal article

Alphabet Projection of Spectra

Journal of Proteome Research.

Contributors: P. Kreitzberg *, M. Bern *, Q. Shu* , F. Yang * & O. Serang *

MeSH headings : Amino Acid Sequence / genetics; Databases, Protein / statistics & numerical data; Glycomics / methods; Mass Spectrometry / methods; Metabolomics / methods; Proteomics / methods; Sequence Analysis, Protein / methods
topics (OpenAlex): Analytical Chemistry and Chromatography; Advanced Proteomics Techniques and Applications; Metabolomics and Mass Spectrometry Studies
TL;DR: A method is proposed that identifies an alphabet of m/z differences that can build large connected graphs from many intense peaks in each spectrum from a collection, and a novel approach is introduced to efficiently find recurring substructures in the de novo graph results. (via Semantic Scholar)
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4. Quality Education (OpenAlex)
Source: ORCID
Added: May 2, 2023

2018 journal article

Antigen 85B peptidomic analysis allows species-specific mycobacterial identification

Clinical Proteomics.

Contributors: W. Zhang *, Q. Shu* , Z. Zhao *, J. Fan *, C. Lyon *, A. Zelazny *, Y. Hu *

topics (OpenAlex): Mycobacterium research and diagnosis; Tuberculosis Research and Epidemiology; Bacteriophages and microbial interactions
TL;DR: Analyzing predicted tryptic peptides of the major mycobacterial antigen Ag85B for their capacity to distinguish Mycobacterium tuberculosis and three NTM species responsible for the majority of pulmonary infections caused by slow-growing myc Cobacteria found one of these species-specific signature peptides in each sample. (via Semantic Scholar)
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15. Life on Land (OpenAlex)
Source: ORCID
Added: May 2, 2023

2018 journal article

N-Linked Glycopeptide Identification Based on Open Mass Spectral Library Search

BioMed Research International.

Contributors: Z. An *, Q. Shu* , H. Lv *, L. Shu *, J. Wang *, F. Yang *, Y. Fu *

MeSH headings : Glycopeptides / analysis; Glycopeptides / chemistry; Glycosylation; Humans; Proteomics; Reproducibility of Results; Search Engine; Tandem Mass Spectrometry
topics (OpenAlex): Glycosylation and Glycoproteins Research; Mass Spectrometry Techniques and Applications; Advanced Proteomics Techniques and Applications
Source: ORCID
Added: May 2, 2023

2016 journal article

Characterization and relative quantification of phospholipids based on methylation and stable isotopic labeling

Journal of Lipid Research.

Contributors: T. Cai *, Q. Shu* , P. Liu*, L. Niu *, X. Guo *, X. Ding *, P. Xue *, Z. Xie * ...

MeSH headings : Cell Line, Tumor; Diazomethane / metabolism; Humans; Isotope Labeling; Lysophospholipids / metabolism; Metabolomics / methods; Methylation; Phospholipids / chemistry; Phospholipids / metabolism; Time Factors
topics (OpenAlex): Sphingolipid Metabolism and Signaling; Lipid Membrane Structure and Behavior; Metabolomics and Mass Spectrometry Studies
TL;DR: The results showed that phosphate methylation allows fast and sensitive identification of the six major PL classes, including their lysophospholipid counterparts, under positive ionization mode, and the isotopic labeling strategy is capable of providing relative quantitation with adequate accuracy, reproducibility, and a coefficient of variation of 9.1%. (via Semantic Scholar)
Source: ORCID
Added: May 2, 2023

2016 journal article

Comparative proteomics reveals abnormal binding of ATGL and dysferlin on lipid droplets from pressure overload-induced dysfunctional rat hearts

Scientific Reports.

Contributors: L. Li*, H. Zhang *, W. Wang *, Y. Hong*, J. Wang *, S. Zhang *, S. Xu *, Q. Shu*  ...

MeSH headings : Animals; Heart / physiology; Heart / physiopathology; Lipase / metabolism; Lipid Droplets / metabolism; Mass Spectrometry; Membrane Proteins / chemistry; Membrane Proteins / metabolism; Muscle Proteins / chemistry; Muscle Proteins / metabolism; Myocardium / metabolism; Pressure; Protein Binding; Protein Domains; Protein Interaction Maps; Proteome / metabolism; Proteomics / methods; Rats, Sprague-Dawley; Reproducibility of Results; Subcellular Fractions / metabolism; Triglycerides / metabolism
topics (OpenAlex): Lipid metabolism and biosynthesis; Adipose Tissue and Metabolism; Pancreatic function and diabetes
TL;DR: This study for the first time reveals the dataset of the heart LD proteome in healthy tissue and the variation of it under cardiac dysfunction, highlighting an association between the altered LD protein localization of dysferlin and ATGL and myocardial dysfunction. (via Semantic Scholar)
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3. Good Health and Well-being (OpenAlex)
Source: ORCID
Added: May 2, 2023

2016 journal article

Evaluation of Different N-Glycopeptide Enrichment Methods for N-Glycosylation Sites Mapping in Mouse Brain

Journal of Proteome Research.

Contributors: C. Zhang *, Z. Ye *, P. Xue *, Q. Shu* , Y. Zhou *, Y. Ji *, Y. Fu *, J. Wang *, F. Yang *

MeSH headings : Amino Acid Motifs; Animals; Binding Sites; Brain / metabolism; Glycopeptides; Glycosylation; Isotope Labeling; Mass Spectrometry / methods; Mice; Oxygen Isotopes; Sensitivity and Specificity
topics (OpenAlex): Glycosylation and Glycoproteins Research; Machine Learning in Bioinformatics; RNA and protein synthesis mechanisms
TL;DR: This work identified a total of 3446 unique glycosylation sites conforming to the N-X-T/S/C; X ≠ P with (18)O labeling in 1597 N-glycoproteins, and showed the highest sensitivity and specificity of the five enrichment methods evaluated. (via Semantic Scholar)
Source: ORCID
Added: May 2, 2023

2015 journal article

Profiling and Relative Quantitation of Phosphoinositides by Multiple Precursor Ion Scanning Based on Phosphate Methylation and Isotopic Labeling

Analytical Chemistry.

Contributors: T. Cai *, Q. Shu* , J. Hou *, P. Liu*, L. Niu *, X. Guo *, C. Liu, F. Yang *

MeSH headings : Animals; Cattle; Chromatography, Liquid; Diazomethane / chemistry; Humans; Isotope Labeling; Lipids / analysis; Male; Methylation; Phosphates / chemistry; Phosphatidylinositols / analysis; Prostatic Neoplasms / metabolism; Spectrometry, Mass, Secondary Ion / methods
topics (OpenAlex): Hemoglobinopathies and Related Disorders; Mass Spectrometry Techniques and Applications; Blood disorders and treatments
TL;DR: DAG(+)-specific MPIS and isotopic labeling based MS analysis of phosphoinositides offers unique advantages over existing approaches and presents a powerful tool for research ofosphoinositide metabolism. (via Semantic Scholar)
Source: ORCID
Added: May 2, 2023

2015 journal article

Proteomic Comparison and MRM-Based Comparative Analysis of Metabolites Reveal Metabolic Shift in Human Prostate Cancer Cell Lines

Journal of Proteome Research.

Contributors: Q. Shu* , T. Cai *, X. Chen *, H. Zhu *, P. Xue *, N. Zhu *, Z. Xie *, S. Wei * ...

MeSH headings : AMP-Activated Protein Kinases / metabolism; Acetyl Coenzyme A / metabolism; Adenylate Kinase / metabolism; Cell Line, Tumor; Chromatography, Liquid / methods; Cluster Analysis; Fatty Acids / metabolism; Humans; Isotope Labeling / methods; Male; Mitochondria / metabolism; Models, Biological; Nucleotides / metabolism; Peptides / metabolism; Prostatic Neoplasms / metabolism; Prostatic Neoplasms / pathology; Proteome / classification; Proteome / metabolism; Proteomics / methods; Receptors, Androgen / metabolism; Reproducibility of Results; Signal Transduction; Tandem Mass Spectrometry / methods
topics (OpenAlex): Prostate Cancer Treatment and Research; Cancer, Lipids, and Metabolism; Cancer, Hypoxia, and Metabolism
TL;DR: Most of the differentially expressed proteins between each pair function in metabolism, indicating a metabolic shift between AS and AI cells, as validated by multiple reaction monitoring (MRM)-based quantification of nucleotides and relative comparison of fatty acids between these cell lines. (via Semantic Scholar)
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UN Sustainable Development Goal Categories
3. Good Health and Well-being (OpenAlex)
Source: ORCID
Added: May 2, 2023

Employment

Updated: May 1st, 2023 18:14

2023 - present

North Carolina State University Raleigh, North Carolina, US
Research Scholar METRIC

2019 - 2023

Tulane University New Orleans, Louisiana, US
Postdoc Fellow Biochemistry and Molecular Biology

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