Works (91)

Updated: November 18th, 2024 11:06

2024 article

Adeno-associated virus genome quantification with amplification-free CRISPR-Cas12a

Hetzler, Z., Marinakos, S. M., Lott, N., Mohammad, N., Lass-Napiorkowska, A., Kolbe, J., … Wei, Q. (2024, March 25). GENE THERAPY, Vol. 3.

By: Z. Hetzler n, S. Marinakos, N. Lott n, N. Mohammad n, A. Lass-Napiorkowska*, J. Kolbe n, L. Turrentine n, D. Fields n ...

UN Sustainable Development Goal Categories
Sources: Web Of Science, NC State University Libraries
Added: April 15, 2024

2024 article

Liquid Metal-Based Biosensors: Fundamentals and Applications

Jamalzadegan, S., Kim, S., Mohammad, N., Koduri, H., Hetzler, Z., Lee, G., … Wei, Q. (2024, January 23). ADVANCED FUNCTIONAL MATERIALS, Vol. 1.

By: S. Jamalzadegan n, S. Kim n, N. Mohammad n, H. Koduri n, Z. Hetzler n, G. Lee n, M. Dickey n, Q. Wei n

author keywords: biosensors; liquid metals; wearable sensors; soft materials; precision health; point-of-care
TL;DR: The recent progress of LM‐based biosensors for personalized healthcare and disease monitoring via both physical and biochemical signaling is survey and the challenges and future directions of LM‐integrated biosensor platforms is discussed. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries, ORCID
Added: February 12, 2024

2024 article

Rapid Detection of Viral, Bacterial, Fungal, and Oomycete Pathogens on Tomatoes with Microneedles, LAMP on a Microfluidic Chip, and Smartphone Device

Shymanovich, T., Saville, A. C., Paul, R., Wei, Q., & Ristaino, J. B. (2024, August 5). PHYTOPATHOLOGY, Vol. 8.

By: T. Shymanovich n, A. Saville n, R. Paul n, Q. Wei n & J. Ristaino n

author keywords: bacterial pathogens; fungal pathogens; oomycetes; pathogen detection
Sources: Web Of Science, NC State University Libraries, ORCID
Added: August 19, 2024

2024 journal article

Ratiometric nonfluorescent CRISPR assay utilizing Cas12a-induced plasmid supercoil relaxation

COMMUNICATIONS CHEMISTRY, 7(1).

By: N. Mohammad n, L. Talton n, S. Dalgan n, Z. Hetzler n, A. Steksova & Q. Wei n

Sources: Web Of Science, NC State University Libraries, ORCID
Added: June 24, 2024

2023 article

A Ratiometric Nonfluorescent CRISPR Assay Utilizing Cas12a-Induced Plasmid Supercoil Relaxation

Mohammad, N., Talton, L., Dalgan, S., & Wei, Q. (2023, December 5).

By: N. Mohammad n, L. Talton n, S. Dalgan n & Q. Wei n

TL;DR: A simple and robust ratiometric signaling strategy using CRISPR-Cas12a-induced supercoil relaxation of dsDNA plasmid reporters is developed, which solves the potential application limitations of previously demonstrated DNA sizing-based CRisPR-Dx without compromising the sensitivity. (via Semantic Scholar)
Source: ORCID
Added: February 22, 2024

2023 journal article

Abaxial leaf surface-mounted multimodal wearable sensor for continuous plant physiology monitoring

SCIENCE ADVANCES, 9(15).

By: G. Lee n, O. Hossain n, S. Jamalzadegan n, Y. Liu n, H. Wang n, A. Saville n, T. Shymanovich n, R. Paul n ...

MeSH headings : Wearable Electronic Devices; Plant Leaves; Temperature; Plant Physiological Phenomena; Volatile Organic Compounds; Plants
Sources: Web Of Science, NC State University Libraries
Added: June 5, 2023

2023 article

CRISPR-Cas Biochemistry and CRISPR-Based Molecular Diagnostics

Weng, Z., You, Z., Yang, J., Mohammad, N., Lin, M., Wei, Q., … Zhang, Y. (2023, February 14). ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, Vol. 2.

By: Z. Weng*, Z. You*, J. Yang*, N. Mohammad n, M. Lin*, Q. Wei n, X. Gao*, Y. Zhang*

author keywords: Amplification-Free Detection; Biosensors; CRISPR-Cas Biochemistry; Molecular Diagnostics; SARS-Cov-2
MeSH headings : Humans; COVID-19; CRISPR-Cas Systems; Pathology, Molecular; SARS-CoV-2; MicroRNAs; Biosensing Techniques; COVID-19 Testing
TL;DR: Four ongoing research hotspots in molecular diagnostics-target preamplification-free detection, microRNA (miRNA) detection, non-nucleic-acid detection, and SARS-CoV-2 detection-are also covered. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Sources: Web Of Science, NC State University Libraries
Added: March 13, 2023

2023 journal article

Low-rate smartphone videoscopy for microsecond luminescence lifetime imaging with machine learning

PNAS NEXUS, 2(10).

Ed(s): R. Reis

author keywords: smartphone videoscopy; V-chopper; time-resolved; lifetime imaging; machine learning
TL;DR: A cost-effective and miniaturized smartphone lifetime imaging system integrated with a pulsed ultraviolet (UV) light-emitting diode (LED) for 2D luminescence lifetime imaging using a videoscopy-based virtual chopper (V-chopper) mechanism combined with machine learning. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries, ORCID
Added: November 6, 2023

2023 journal article

Precise in-field molecular diagnostics of crop diseases by smartphone-based mutation-resolved pathogenic RNA analysis

NATURE COMMUNICATIONS, 14(1).

By: T. Zhang*, Q. Zeng*, F. Ji*, H. Wu*, R. Ledesma-Amaro*, Q. Wei n, H. Yang*, X. Xia* ...

TL;DR: By detecting fungal RNA and mutations, the approach allows to distinguish viable and dead pathogens and to sensitively identify mutation-carrying fungicide-resistant isolates, providing fundamental information for precision crop disease management. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries, ORCID
Added: August 21, 2023

2023 journal article

Single-use biosensors for biomanufacturing: Perspective on the state-of-the-art

CURRENT OPINION IN BIOMEDICAL ENGINEERING, 28.

By: Z. Hetzler n, N. Lott n, A. Poonam n, S. Dalgan n & Q. Wei n

author keywords: Biomanufacturing; Gene therapy; Process monitoring; Process analytical technology (PAT); Single-use sensors
Sources: Web Of Science, NC State University Libraries, ORCID
Added: January 2, 2024

2023 article

Unidirectional trans-cleaving behavior of CRISPR-Cas12a unlocks for an ultrasensitive assay using hybrid DNA reporters containing a 3' toehold

Mohammad, N., Talton, L., Hetzler, Z., Gongireddy, M., & Wei, Q. (2023, August 31). NUCLEIC ACIDS RESEARCH, Vol. 8.

By: N. Mohammad n, L. Talton n, Z. Hetzler n, M. Gongireddy n & Q. Wei n

TL;DR: This study not only unfolded new insight into the trans-cleavage behavior of Cas12a but also demonstrated a sensitive CRISPR-Cas12a assay by using a hybrid dsDNA reporter molecule. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries, ORCID
Added: September 18, 2023

2022 journal article

<p>Sub-picomolar lateral flow antigen detection with two-wavelength imaging of composite nanoparticles</p>

BIOSENSORS & BIOELECTRONICS, 207.

By: B. Miller*, M. Thomas*, M. Banner*, J. Kim*, Y. Chen*, Q. Wei n, D. Tseng*, Z. Goeroecs ...

author keywords: Lateral flow; Imaging; Nanoparticles; Biosensors
MeSH headings : Biosensing Techniques / methods; Biotin; Gold; Limit of Detection; Metal Nanoparticles
UN Sustainable Development Goal Categories
Sources: Web Of Science, NC State University Libraries
Added: May 16, 2022

2022 article

A Sensitive and Nonoptical CRISPR Detection Mechanism by Sizing Double-Stranded lambda DNA Reporter

Mohammad, N., Katkam, S. S., & Wei, Q. (2022, November 10). ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, Vol. 11.

By: N. Mohammad n, S. Katkam n & Q. Wei n

author keywords: Biosensors; CRISPR-Cas12a; DNA Sizing; DNA-Cleavage; lambda DNA
MeSH headings : CRISPR-Cas Systems; DNA; DNA Cleavage; DNA, Single-Stranded; Biosensing Techniques
TL;DR: A simple, inexpensive, nonoptical, and sensitive CRISPR‐Cas12a‐based sensing platform to detect ssDNA targets by sizing double‐stranded λ DNA as novel report molecules, unlocking the possibility of using a variety of dsDNA asCRISPR reporters. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Sources: Web Of Science, NC State University Libraries, ORCID
Added: November 21, 2022

2022 journal article

A Sensitive and Nonoptical CRISPR Detection Mechanism by Sizing Double‐Stranded λ DNA Reporter

Angewandte Chemie.

By: N. Mohammad n, S. Katkam n & Q. Wei n

Source: ORCID
Added: December 2, 2022

2022 journal article

Creating hierarchically porous banana paper-metal organic framework (MOF) composites with multifunctionality

APPLIED MATERIALS TODAY, 28.

By: S. Wang n, T. Pirzada n, W. Xie n, E. Barbieri n, O. Hossain n, C. Opperman n, L. Pal n, Q. Wei n, G. Parsons n, S. Khan n

author keywords: lignocellulosic biomass; Banana fiber; Gas sorption; Antibacterial matrix; MOF-fiber composites; VOC sensor
Sources: Web Of Science, NC State University Libraries, ORCID
Added: May 22, 2022

2022 journal article

Flexible sensor patch for continuous carbon dioxide monitoring

FRONTIERS IN CHEMISTRY, 10.

author keywords: CO2; in-line sensor; flexible sensor; process analytical technology; cell culture; biomanufacturing
Sources: Web Of Science, NC State University Libraries
Added: March 6, 2023

2022 journal article

Integrating charge mobility, stability and stretchability within conjugated polymer films for stretchable multifunctional sensors

NATURE COMMUNICATIONS, 13(1).

By: S. Son*, G. Lee n, H. Wang n, S. Samson*, Q. Wei n, Y. Zhu n, W. You*

Sources: Web Of Science, NC State University Libraries, ORCID
Added: June 13, 2022

2022 article

Recent Advances in Clustered Regularly Interspaced Short Palindromic Repeats-Based Biosensors for Point-of-Care Pathogen Detection

Mohammad, N., Katkam, S. S., & Wei, Q. (2022, July 18). CRISPR JOURNAL, Vol. 7.

By: N. Mohammad n, S. Katkam n & Q. Wei n

MeSH headings : Biosensing Techniques; CRISPR-Cas Systems / genetics; Gene Editing / methods; Plants; Point-of-Care Systems
TL;DR: This review highlights recent efforts in configuring CRISPR technology as an efficient diagnostic tool for pathogen detection, including the development of new signaling mechanisms, elimination of preamplification and sample pretreatment steps, and miniaturization ofCRISPR reactions on digital assay chips and lateral flow devices. (via Semantic Scholar)
UN Sustainable Development Goal Categories
9. Industry, Innovation and Infrastructure (OpenAlex)
Sources: Web Of Science, NC State University Libraries, ORCID
Added: August 1, 2022

2022 article

Surface Wrinkling for Flexible and Stretchable Sensors

Lee, G., Zarei, M., Wei, Q., Zhu, Y., & Lee, S. G. (2022, September 1). SMALL, Vol. 9.

By: G. Lee n, M. Zarei*, Q. Wei n, Y. Zhu n & S. Lee*

author keywords: flexibility; sensors; stretchability; surface wrinkling; wearable devices
MeSH headings : Wearable Electronic Devices; Electronics; Temperature
UN Sustainable Development Goal Categories
Sources: Web Of Science, NC State University Libraries, ORCID
Added: September 12, 2022

2021 journal article

A smartphone-read ultrasensitive and quantitative saliva test for COVID-19

SCIENCE ADVANCES, 7(2).

By: B. Ning*, T. Yu n, S. Zhang n, Z. Huang*, D. Tian*, Z. Lin*, A. Niu*, N. Golden* ...

MeSH headings : Animals; COVID-19 / diagnosis; COVID-19 Nucleic Acid Testing; CRISPR-Cas Systems; Chlorocebus aethiops; Computer Simulation; Female; Humans; Limit of Detection; Macaca mulatta; Male; Molecular Diagnostic Techniques / instrumentation; Point-of-Care Testing; Reverse Transcriptase Polymerase Chain Reaction; Saliva / virology; Sensitivity and Specificity; Smartphone; Vero Cells; Viral Load
TL;DR: An isolation-free smartphone-based saliva assay that can diagnose COVID-19 cases and quantify viral load within 15 min and exhibited a limit of detection below that of the RT-PCR reference assay is described. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: February 8, 2021

2021 journal article

Addressable nanoantennas with cleared hotspots for single-molecule detection on a portable smartphone microscope

NATURE COMMUNICATIONS, 12(1).

By: K. Trofymchuk*, V. Glembockyte*, L. Grabenhorst*, F. Steiner*, C. Vietz*, C. Close*, M. Pfeiffer*, L. Richter* ...

MeSH headings : DNA / chemistry; Drug Resistance, Bacterial; Fluorescence; Humans; Klebsiella pneumoniae / drug effects; Male; Microscopy; Nanostructures; Nanotechnology; Point-of-Care Testing; Serum / chemistry; Smartphone
Sources: Web Of Science, NC State University Libraries
Added: March 15, 2021

2021 journal article

Cellphone enabled point-of-care assessment of breast tumor cytology and molecular HER2 expression from fine-needle aspirates

NPJ BREAST CANCER, 7(1).

By: D. Joh*, J. Heggestad*, S. Zhang n, G. Anderson*, J. Bhattacharyya*, S. Wardell*, S. Wall*, A. Cheng* ...

TL;DR: It was found that FNA cytology specimens imaged by the EpiView-D4 were adequate for assessment of lesional cellularity and tumor content, and the device could reliably distinguish between HER2 expression levels across multiple different cell lines and animal xenografts. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: July 19, 2021

2021 article

Coupling smartphone and CRISPR-Cas12a for digital and multiplexed nucleic acid detection

Yu, T., Zhang, S., Matei, R., Marx, W., Beisel, C. L., & Wei, Q. (2021, July 23). AICHE JOURNAL, Vol. 7.

By: T. Yu n, S. Zhang n, R. Matei*, W. Marx n, C. Beisel n & Q. Wei n

author keywords: Cas12a; CRISPR diagnostics; digital assay; point-of-care diagnostics; smartphone device
UN Sustainable Development Goal Categories
Sources: Web Of Science, NC State University Libraries, ORCID
Added: August 2, 2021

2021 article

Editorial: Array-Based Sensing Techniques for Clinical, Agricultural Biotechnology, and Environmental Analysis

Li, Z., Wei, Q., & Han, J. (2021, March 12). FRONTIERS IN CHEMISTRY, Vol. 9.

By: Z. Li*, Q. Wei n & J. Han*

author keywords: array-based sensing; pattern-recognition; optoelectronic nose; optical sensing; biosensing
TL;DR: A Research Topic in Frontiers in Chemistry entitled “Array-Based Sensing Techniques for Clinical, Agricultural Biotechnology, and Environmental Analysis” was launched, which covered the use of colorimetric, fluorometric, and electrochemical sensor arrays for bioengineering monitoring, bioanalysis, and many other clinical applications. (via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger (OpenAlex)
Sources: Web Of Science, NC State University Libraries
Added: April 5, 2021

2021 article

Emerging Wearable Sensors for Plant Health Monitoring

Lee, G., Wei, Q., & Zhu, Y. (2021, October 7). ADVANCED FUNCTIONAL MATERIALS, Vol. 10.

By: G. Lee n, Q. Wei n & Y. Zhu n

author keywords: plant health monitoring; plant sensors; plant stress; volatile organic compounds; wearable sensors
UN Sustainable Development Goal Categories
Sources: Web Of Science, NC State University Libraries
Added: October 18, 2021

2021 journal article

Integrated microneedle-smartphone nucleic acid amplification platform for in-field diagnosis of plant diseases

BIOSENSORS & BIOELECTRONICS, 187.

author keywords: Plant disease diagnostics; Nucleic acid extraction; Microneedle; Isothermal amplification; Smartphone
MeSH headings : Biosensing Techniques; Molecular Diagnostic Techniques; Nucleic Acid Amplification Techniques; Plant Diseases; Smartphone
TL;DR: It is found that the MN patch containing as few as a single needle tip successfully extracted enough RNA for RT-PCR or RT-LAMP analysis, and MN-extracted RNA samples remained stable on MN surfaces for up to three days. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Sources: Web Of Science, NC State University Libraries, ORCID
Added: June 14, 2021

2021 review

Plant pest surveillance: from satellites to molecules

[Review of ]. EMERGING TOPICS IN LIFE SCIENCES, 5(2), 275–287.

By: G. Silva*, J. Tomlinson*, N. Onkokesung*, S. Sommer*, L. Mrisho*, J. Legg*, I. Adams*, Y. Gutierrez-Vazquez* ...

MeSH headings : Climate Change; Ecosystem; Plant Weeds
TL;DR: This review will explore the different technologies that are enabling this advancing capability and discuss the drivers that will shape its future deployment. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: June 21, 2021

2021 journal article

Real-time monitoring of plant stresses via chemiresistive profiling of leaf volatiles by a wearable sensor

MATTER, 4(7), 2553–2570.

By: Z. Li n, Y. Liu n, O. Hossain n, R. Paul n, S. Yao n, S. Wu n, J. Ristaino n, Y. Zhu n, Q. Wei n

TL;DR: A leaf-attachable chemiresistive sensor array for real-time fingerprinting of volatile organic compounds (VOCs) that permits noninvasive and early diagnosis of plant diseases, such as late blight caused by Phytophthora infestans is reported. (via Semantic Scholar)
UN Sustainable Development Goal Categories
16. Peace, Justice and Strong Institutions (OpenAlex)
Sources: Web Of Science, NC State University Libraries
Added: July 26, 2021

2021 article

Recent Advances in Aptamer-Based Biosensors for Global Health Applications

ANNUAL REVIEW OF BIOMEDICAL ENGINEERING, VOL 23, 2021, Vol. 23, pp. 433–459.

By: L. Stanciu*, Q. Wei n, A. Barui* & N. Mohammad n

author keywords: aptamer recognition; biosensors; point-of-care diagnostics; global health; optical sensors; electrochemical sensors
MeSH headings : Aptamers, Nucleotide; Biosensing Techniques; Global Health; Proteins
TL;DR: A description of general aptasensing principles is provided and examples of recent reports of diagnostics-related applications, including detection of proteins and small molecules, circulating cancer cells, whole-cell pathogens, extracellular vesicles, and tissue diagnostics are discussed. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: August 2, 2021

2021 journal article

Single-molecule and particle detection on true portable microscopy platforms

SENSORS AND ACTUATORS REPORTS, 4.

By: L. Skolrood n, Y. Wang n, S. Zhang n & Q. Wei n

author keywords: Single-molecule imaging; Point-of-care diagnostics; Smartphone; Fluorescence microscopy; On-chip microscopy; SERS
Sources: Web Of Science, NC State University Libraries
Added: January 24, 2022

2021 review

Smartphone videoscopy: Recent progress and opportunities for biosensing

[Review of ]. ADVANCED OPTICAL TECHNOLOGIES, 10(2), 123–138.

By: Y. Wang n, S. Zhang n & Q. Wei n

author keywords: biosensing; fluorescence microscopy; point-of-care diagnostics; smartphone videoscopy; time-resolved
TL;DR: The recent development of smartphone videoscopy is summarized based on different POC applications and how these time-dependent measurements could open up new opportunities for POC diagnostics are discussed in detail. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries, ORCID
Added: July 26, 2021

2021 journal article

The persistent threat of emerging plant disease pandemics to global food security

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 118(23).

author keywords: emerging plant disease; plant pathology; food security
MeSH headings : Climate Change; Ecosystem; Food Security; Humans; Plant Diseases
TL;DR: An integrated research agenda that could help mitigate future plant disease pandemics is described that includes disease surveillance and improved detection technologies including pathogen sensors and predictive modeling and data analytics. (via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger (Web of Science)
13. Climate Action (Web of Science)
Sources: Web Of Science, NC State University Libraries, ORCID
Added: August 23, 2021

2021 review

Trends and applications in plant volatile sampling and analysis

[Review of ]. PLANT JOURNAL, 106(2), 314–325.

By: D. Tholl*, O. Hossain n, A. Weinhold*, U. Rose* & Q. Wei n

author keywords: volatile organic compound; plant volatile analysis; SPME; SBSE; GC&#8208; MS; PTR&#8208; MS; volatilomics; phenotyping; e&#8208; nose; VOC sensor
MeSH headings : Plant Components, Aerial / chemistry; Plant Diseases; Plant Roots / chemistry; Plants / chemistry; Plants / metabolism; Soil; Volatile Organic Compounds / analysis
TL;DR: Advances in the use of small, inexpensive sampling devices and methods to monitor plant VOC emissions in the belowground environment are shown and latest trends in real-time measurements of volatilomes in plant phenotyping are addressed. (via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger (Web of Science; OpenAlex)
13. Climate Action (Web of Science)
15. Life on Land (Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: June 10, 2021

2020 journal article

Advances in point-of-care nucleic acid extraction technologies for rapid diagnosis of human and plant diseases

BIOSENSORS & BIOELECTRONICS, 169.

By: R. Paul n, E. Ostermann n & Q. Wei n

author keywords: Point-of-care diagnostics; DNA/RNA extraction; Nucleic acid amplification; Raw samples; Infectious diseases; Plant diseases
MeSH headings : Betacoronavirus / isolation & purification; COVID-19; Chemical Fractionation / instrumentation; Chemical Fractionation / methods; Communicable Diseases / diagnosis; Communicable Diseases / microbiology; Communicable Diseases / parasitology; Communicable Diseases / virology; Coronavirus Infections / diagnosis; Coronavirus Infections / virology; Equipment Design; Humans; Lab-On-A-Chip Devices; Nucleic Acid Amplification Techniques / instrumentation; Nucleic Acid Amplification Techniques / methods; Nucleic Acids / blood; Nucleic Acids / isolation & purification; Nucleic Acids / urine; Pandemics; Plant Diseases / microbiology; Plant Diseases / parasitology; Plant Diseases / virology; Pneumonia, Viral / diagnosis; Pneumonia, Viral / virology; Point-of-Care Systems; SARS-CoV-2
TL;DR: This review focuses on novel devices or methods that have demonstrated applicability and robustness for the isolation of high-quality nucleic acid from complex raw samples, such as human blood, saliva, sputum, nasal swabs, urine, and plant tissues that would pave the road for the “sample-in-result-out” diagnosis of human and plant diseases, especially in remote or resource-limited settings. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: November 16, 2020

2020 review

Agricultural nanodiagnostics for plant diseases: recent advances and challenges

[Review of ]. NANOSCALE ADVANCES, 2(8), 3083–3094.

By: Z. Li n, T. Yu n, R. Paul n, J. Fan*, Y. Yang* & Q. Wei n

UN Sustainable Development Goal Categories
2. Zero Hunger (OpenAlex)
Sources: Web Of Science, NC State University Libraries, ORCID
Added: August 31, 2020

2020 review

Portable Analytical Techniques for Monitoring Volatile Organic Chemicals in Biomanufacturing Processes: Recent Advances and Limitations

[Review of ]. FRONTIERS IN CHEMISTRY, 8.

By: X. Chen*, R. Hu*, L. Hu*, Y. Huang*, W. Shi*, Q. Wei n, Z. Li*

author keywords: biomanufacturing; volatile organic chemicals; bioprocess assessments; analytical methods; electronic noses; sensors
TL;DR: A comprehensive summary toward the use of mainstream techniques for bioprocess monitoring is critically reviewed here, which illustrates the instrumental and procedural advances and limitations of several major analytical tools in biomanufacturing applications. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: October 26, 2020

2019 journal article

Aptamer-Based Fluorescent Sensor Array for Multiplexed Detection of Cyanotoxins on a Smartphone

ANALYTICAL CHEMISTRY, 91(16), 10448–10457.

By: Z. Li n, S. Zhang n, T. Yu n, Z. Dai n & Q. Wei n

MeSH headings : Alkaloids; Aptamers, Nucleotide / chemistry; Bacterial Toxins / analysis; Biosensing Techniques / instrumentation; Biosensing Techniques / methods; Cyanobacteria Toxins; DNA, Single-Stranded / chemistry; Fluorescence; Fresh Water / chemistry; Humans; Lab-On-A-Chip Devices; Lakes / chemistry; Limit of Detection; Marine Toxins; Microarray Analysis; Microcystins / analysis; Peptides, Cyclic / analysis; Smartphone; Tropanes / analysis; Uracil / analogs & derivatives; Uracil / analysis; Water Pollutants, Chemical / analysis
TL;DR: A differential fluorescent sensor array made of aptamers and single-stranded DNA dyes for multiplexed detection and discrimination of four common cyanotoxins with an ordinary smartphone within 5 minutes of reaction. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Sources: Web Of Science, NC State University Libraries, ORCID
Added: September 16, 2019

2019 journal article

Benchmarking Smartphone Fluorescence-Based Microscopy with DNA Origami Nanobeads: Reducing the Gap toward Single-Molecule Sensitivity

ACS OMEGA, 4(1), 637–642.

TL;DR: The use of DNA origami nanobeads with predefined numbers of fluorophores to quantify the sensitivity of a smartphone-based fluorescence microscope in terms of the minimum number of detectable molecules per diffraction-limited spot is reported. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries, ORCID
Added: March 25, 2019

2019 journal article

Detection of Phytophthora infestans by Loop-Mediated Isothermal Amplification, Real-Time LAMP, and Droplet Digital PCR

PLANT DISEASE, 104(3), 708–716.

By: J. Ristaino n, A. Saville n, R. Paul n, D. Cooper* & Q. Wei n

author keywords: oomycetes; pathogen detection; Phytophthora infestans; vegetables
MeSH headings : Solanum lycopersicum; Nucleic Acid Amplification Techniques; Phytophthora infestans; Polymerase Chain Reaction; Solanum tuberosum
TL;DR: The loop-mediated isothermal amplification (LAMP) assay was specific for P. infestans on potato and tomato and did not amplify other potato- or tomato-infecting Phytophthora species or other fungal and bacterial pathogens that infect potato and Tomato. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: April 6, 2020

2019 journal article

Extraction of Plant DNA by Microneedle Patch for Rapid Detection of Plant Diseases

ACS Nano, 13(6), 6540–6549.

By: R. Paul n, A. Saville n, J. Hansel n, Y. Ye n, C. Ball n, A. Williams n, X. Chang*, G. Chen n ...

author keywords: plant disease; DNA extraction; microneedle patch; Phytophthora infestans; nucleic acid amplification; point-of-care diagnostics
MeSH headings : DNA Barcoding, Taxonomic / instrumentation; DNA Barcoding, Taxonomic / methods; DNA, Fungal / chemistry; DNA, Fungal / genetics; Solanum lycopersicum / microbiology; Metagenomics / instrumentation; Metagenomics / methods; Needles; Phytophthora / genetics; Phytophthora / pathogenicity; Plant Diseases / microbiology; Plant Leaves / chemistry; Plant Leaves / microbiology
TL;DR: A simple, cell-lysis-free, and purification-free DNA extraction method that could be a transformative approach to facilitate rapid sample preparation for molecular diagnosis of various plant diseases directly in the field is developed. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Sources: Web Of Science, NC State University Libraries, Crossref
Added: July 15, 2019

2019 journal article

Non-invasive plant disease diagnostics enabled by smartphone-based fingerprinting of leaf volatiles

Nature Plants.

By: Z. Li n, R. Paul n, T. Tis n, A. Saville n, J. Hansel n, T. Yu n, J. Ristaino n, Q. Wei n

MeSH headings : Mobile Applications; Phytophthora infestans / physiology; Plant Diseases / microbiology; Plant Leaves / chemistry; Smartphone; Solanum tuberosum / microbiology; Volatile Organic Compounds / analysis
TL;DR: A cost-effective smartphone-based volatile organic compound (VOC) fingerprinting platform that allows non-invasive diagnosis of late blight caused by Phytophthora infestans by monitoring characteristic leaf volatile emissions in the field. (via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger (OpenAlex)
Source: ORCID
Added: April 10, 2020

2018 journal article

Deep Learning Enables High-Throughput Analysis of Particle-Aggregation-Based Biosensors Imaged Using Holography

ACS PHOTONICS, 6(2), 294–301.

author keywords: particle clustering assay; biosensing; virus sensing; deep learning; digital holography; computational microscopy
TL;DR: This work presents a sensitive and automated readout method for aggregation-based assays using a wide-field lens-free on-chip microscope, with the ability to rapidly analyze and quantify microscopic particle aggregation events in 3D, using deep learning-based holographic image reconstruction. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries, ORCID
Added: March 11, 2019

2018 journal article

Ferrodrop Dose-Optimized Digital Quantification of Biomolecules in Low-Volume Samples

Analytical Chemistry, 90(15), 8881–8888.

MeSH headings : DNA / analysis; Emulsions / chemistry; Equipment Design; Magnetite Nanoparticles / chemistry; Microfluidic Analytical Techniques / economics; Microfluidic Analytical Techniques / instrumentation; Nucleic Acid Amplification Techniques / economics; Nucleic Acid Amplification Techniques / instrumentation; Sample Size
TL;DR: An approach to estimate the concentration of a biomolecule in a solution by sampling several nanoliter-scale volumes and determining if the volumes contain any biomolecules is presented, which demonstrates detection of lambda DNA molecules down to 25 copies per microliter. (via Semantic Scholar)
Sources: Crossref, NC State University Libraries, ORCID
Added: February 5, 2020

2018 review

Plasmonic molecular assays: Recent advances and applications for mobile health

[Review of ]. NANO RESEARCH, 11(10), 5439–5473.

By: T. Yu n & Q. Wei n

Contributors: T. Yu n & Q. Wei n

author keywords: plasmonics; molecular assay; point-of-care diagnostics; mobile health; imaging; sensing
TL;DR: This review highlights the recent advances and applications of plasmonic technologies for highly sensitive protein and nucleic acid biomarker detection and focuses on the implementation and penetration of various plasMonic technologies in conventional molecular diagnostic assays, and how such modification has resulted in simpler, faster, and more sensitive alternatives that are suited for point-of-use. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Sources: Web Of Science, ORCID, NC State University Libraries
Added: October 19, 2018

2018 conference paper

Plasmonics Improves the Sensitivity of Smartphone Fluorescence Microscopy

Conference on Lasers and Electro-Optics.

UN Sustainable Development Goal Categories
7. Affordable and Clean Energy (OpenAlex)
Source: ORCID
Added: March 21, 2019

2018 review

Smartphone-based clinical diagnostics: towards democratization of evidence-based health care

[Review of ]. JOURNAL OF INTERNAL MEDICINE, 285(1), 19–39.

By: I. Hernandez-Neuta*, F. Neumann*, J. Brightmeyer n, T. Tis n, N. Madaboosi*, Q. Wei n, A. Ozcan*, M. Nilsson*

Contributors: I. Hernández-Neuta*, F. Neumann*, J. Brightmeyer n, T. Ba Tis n, N. Madaboosi*, Q. Wei n, A. Ozcan*, M. Nilsson*

author keywords: Biosensors; Diagnostics; Digital pathology; Lab-on-a-chip; Smartphones
MeSH headings : Biosensing Techniques; Cytological Techniques; Evidence-Based Practice; Humans; Lab-On-A-Chip Devices; Microfluidics; Mobile Applications; Pathology, Molecular; Point-of-Care Systems; Printing, Three-Dimensional; Smartphone
TL;DR: An overview of the emerging field of clinical smartphone diagnostics and its contributing technologies, as well as their wide range of areas of application, which span from haematology to digital pathology and rapid infectious disease diagnostics are provided. (via Semantic Scholar)
Sources: ORCID, Web Of Science
Added: January 7, 2019

2017 journal article

Enzyme-Free Nucleic Acid Amplification Assay Using a Cellphone-Based Well Plate Fluorescence Reader

ANALYTICAL CHEMISTRY, 90(1), 690–695.

By: D. Kim, Q. Wei n, D. Kim, D. Tseng, J. Zhang, E. Pan, O. Garner, A. Ozcan, D. Di Carlo

Contributors: D. Kim, Q. Wei n, D. Kim, D. Tseng, J. Zhang, E. Pan, O. Garner, A. Ozcan, D. Di Carlo

MeSH headings : Base Sequence; Biological Assay / methods; Cell Line, Tumor; Cell Phone; Fluorescent Dyes / chemistry; Humans; Limit of Detection; MicroRNAs / blood; MicroRNAs / genetics; Nucleic Acid Amplification Techniques / methods; Nucleic Acid Hybridization; Oligodeoxyribonucleotides / genetics; Orthomyxoviridae / genetics; Point-of-Care Systems
TL;DR: This work presents a system that utilizes a series of toehold-triggered hybridization/displacement reactions that are designed to convert a given amount of RNA molecules into an amplified amount of signaling molecules without any washing steps or thermocycling, and demonstrates the success of this approach with two analytes of diagnostic importance. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Sources: Web Of Science, ORCID, NC State University Libraries
Added: August 6, 2018

2017 journal article

Highly Stable and Sensitive Nucleic Acid Amplification and Cell-Phone-Based Readout

ACS Nano, 11(3), 2934–2943.

author keywords: point-of-care assay; loop-mediated isothermal amplification; mobile phone fluorescence microscopy; nucleic acid detection; intercalating dyes
MeSH headings : Bacteriophage lambda / chemistry; Cell Phone / economics; DNA / analysis; Fluorescence; Intercalating Agents / chemistry; Molecular Structure; Naphthalenesulfonates / chemistry; Nucleic Acid Amplification Techniques / economics; Nucleic Acid Amplification Techniques / instrumentation; Point-of-Care Systems / economics; Spectrometry, Fluorescence / economics; Spectrometry, Fluorescence / instrumentation
TL;DR: This work identified a chemical additive, hydroxynaphthol blue, that both stabilizes and significantly enhances intercalator-based fluorescence readout of nucleic acid concentration, and developed a cost-effective fiber-optic bundle- based fluorescence microplate reader integrated onto a mobile phone. (via Semantic Scholar)
Source: ORCID
Added: March 21, 2019

2017 journal article

Inkjet-printed point-of-care immunoassay on a nanoscale polymer brush enables subpicomolar detection of analytes in blood

Proceedings of the National Academy of Sciences of the United States of America, 114(34), E7054–E7062.

By: D. Joh*, A. Hucknall*, Q. Wei*, K. Mason*, M. Lund*, C. Fontes*, R. Hill*, R. Blair* ...

Contributors: D. Joh*, A. Hucknall*, Q. Wei*, K. Mason*, M. Lund*, C. Fontes*, R. Hill*, R. Blair* ...

author keywords: nanoscale; nonfouling; polymer brush; inkjet printing; point of care
MeSH headings : Biomarkers / blood; Blood Chemical Analysis / instrumentation; Blood Chemical Analysis / methods; Equipment Design; Humans; Immunoassay / instrumentation; Immunoassay / methods; Immunoglobulin G / blood; Immunoglobulin M / blood; Leptin / blood; Point-of-Care Systems; Polymers / chemistry; Printing
TL;DR: An inkjet-printed assay platform built on a nonfouling, nanoscale polymer brush, which eliminates nonspecific binding and has the potential to democratize access to high-performance immunoassays in resource-limited settings without sacrificing their performance is introduced. (via Semantic Scholar)
UN Sustainable Development Goal Categories
16. Peace, Justice and Strong Institutions (OpenAlex)
Source: ORCID
Added: March 21, 2019

2017 journal article

Plasmonics Enhanced Smartphone Fluorescence Microscopy

SCIENTIFIC REPORTS, 7(1).

By: Q. Wei*, G. Acuna*, S. Kim*, C. Vietz*, D. Tseng*, J. Chae*, D. Shir*, W. Luo* ...

Contributors: Q. Wei*, G. Acuna*, S. Kim*, C. Vietz*, D. Tseng*, J. Chae*, D. Shir*, W. Luo*, P. Tinnefeld*, A. Ozcan*

MeSH headings : Glass / chemistry; Image Enhancement / instrumentation; Image Enhancement / methods; Microscopy, Fluorescence / instrumentation; Microscopy, Fluorescence / methods; Point-of-Care Testing; Quantum Dots / chemistry; Silver / chemistry; Smartphone
Sources: Web Of Science, ORCID, NC State University Libraries
Added: August 6, 2018

2017 journal article

Targeted DNA sequencing and in situ mutation analysis using mobile phone microscopy

Nature Communications, 8.

By: M. Kühnemund*, Q. Wei*, E. Darai*, Y. Wang*, H. Iván*, Z. Yang*, D. Tseng*, A. Ahlford* ...

Contributors: M. Kühnemund*, Q. Wei*, E. Darai*, Y. Wang*, H. Iván*, Z. Yang*, D. Tseng*, A. Ahlford* ...

MeSH headings : Cell Phone / statistics & numerical data; DNA / genetics; Humans; Microscopy; Mutation; Neoplasms / diagnosis; Neoplasms / genetics; Pathology, Molecular / instrumentation; Pathology, Molecular / methods; Proto-Oncogene Proteins p21(ras) / genetics; Sequence Analysis, DNA / instrumentation; Sequence Analysis, DNA / methods
TL;DR: It is demonstrated that targeted next-generation DNA sequencing reactions and in situ point mutation detection assays in preserved tumour samples can be imaged and analysed using mobile phone microscopy, achieving a new milestone for tele-medicine technologies. (via Semantic Scholar)
Source: ORCID
Added: March 21, 2019

2016 conference paper

A smartphone-based microplate reader for point-of-care ELISA quantification

2016 Conference on Lasers and Electro-Optics, CLEO 2016. http://www.scopus.com/inward/record.url?eid=2-s2.0-85010702301&partnerID=MN8TOARS

By: B. Berg, B. Cortazar, D. Tseng, H. Ozkan, S. Feng, Q. Wei, R. Chan, J. Burbano ...

Contributors: B. Berg, B. Cortazar, D. Tseng, H. Ozkan, S. Feng, Q. Wei, R. Chan, J. Burbano ...

Source: ORCID
Added: April 10, 2020

2016 conference paper

Single DNA imaging and length quantification through a mobile phone microscope

Progress in Biomedical Optics and Imaging - Proceedings of SPIE, 9699.

By: Q. Wei*, W. Luo*, S. Chiang*, T. Kappel*, C. Mejia*, D. Tseng*, R. Chan*, E. Yan* ...

Contributors: Q. Wei*, W. Luo*, S. Chiang*, T. Kappel*, C. Mejia*, D. Tseng*, R. Chan*, E. Yan* ...

author keywords: mobile phone microscopy; fluorescence; DNA imaging; point of care; mobile health; single-molecule imaging; telemedicine; mobile health
TL;DR: A mobile-phone integrated fluorescence microscopy platform that allows imaging and sizing of single DNA molecules that are stretched on a chip and can be very useful for various diagnostic applications including the detection of disease-specific genes and quantification of copy-number-variations at POC settings. (via Semantic Scholar)
Source: ORCID
Added: March 21, 2019

2015 journal article

Cellphone-Based Hand-Held Microplate Reader for Point-of-Care Testing of Enzyme-Linked Immunosorbent Assays

ACS Nano, 9(8), 7857–7866.

By: B. Berg*, B. Cortazar*, D. Tseng*, H. Ozkan*, S. Feng*, Q. Wei*, R. Chan*, J. Burbano* ...

Contributors: B. Berg*, B. Cortazar*, D. Tseng*, H. Ozkan*, S. Feng*, Q. Wei*, R. Chan*, J. Burbano* ...

author keywords: ELISA; immunoassay point-of-care; microplate reader; smartphone; vaccination telemedicine
MeSH headings : Antibodies, Viral / blood; Cell Phone / instrumentation; Colorimetry / economics; Colorimetry / instrumentation; Colorimetry / methods; Computers, Handheld / economics; Enzyme-Linked Immunosorbent Assay / economics; Enzyme-Linked Immunosorbent Assay / instrumentation; Enzyme-Linked Immunosorbent Assay / methods; Herpes Genitalis / blood; Herpes Genitalis / diagnosis; Herpes Genitalis / immunology; Herpes Simplex / blood; Herpes Simplex / diagnosis; Herpes Simplex / immunology; Humans; Immunoglobulin G / blood; Machine Learning; Measles / blood; Measles / diagnosis; Measles / immunology; Mobile Applications; Mumps / blood; Mumps / diagnosis; Mumps / immunology; Optical Fibers; Point-of-Care Systems / economics; Point-of-Care Testing; Sensitivity and Specificity
TL;DR: This cost-effective and hand-held platform could assist health-care professionals to perform high-throughput disease screening or tracking of vaccination campaigns at the point-of-care, even in resource-poor and field-settings. (via Semantic Scholar)
UN Sustainable Development Goal Categories
1. No Poverty (OpenAlex)
Source: ORCID
Added: March 21, 2019

2015 journal article

Democratization of Nanoscale Imaging and Sensing Tools Using Photonics

Analytical Chemistry, 87(13), 6434–6445.

By: E. McLeod*, Q. Wei* & A. Ozcan*

Contributors: E. McLeod*, Q. Wei* & A. Ozcan*

MeSH headings : Microscopy, Fluorescence; Nanotechnology; Optics and Photonics
TL;DR: Some of the recent progress toward making optical nanoscale measurement tools more cost-effective, field-portable, and accessible to a significantly larger group of researchers and educators is reviewed. (via Semantic Scholar)
Source: ORCID
Added: March 21, 2019

2015 conference paper

Field-portable smartphone microscopy platform for widefield imaging and sizing of single DNA molecules

CLEO: Applications and Technology, CLEO-AT 2015, 2015-August.

By: Q. Wei*, W. Luo*, S. Chiang*, T. Kappe*, C. Mejia*, D. Tseng*, Yan, L. Chan ...

Contributors: Q. Wei*, W. Luo*, S. Chiang*, T. Kappe*, C. Mejia*, D. Tseng*, Yan, L. Chan ...

Source: ORCID
Added: March 21, 2019

2015 journal article

Mobile Phone-Based Microscopy, Sensing, and Diagnostics

IEEE Journal on Selected Topics in Quantum Electronics, 22(3).

Contributors: J. Contreras-Naranjo*, Q. Wei* & A. Ozcan*

author keywords: Diagnostics; mobile health; telemedicine; microscopy; sensing
TL;DR: Recent advances in the use of mobile phone-based imaging and sensing techniques for point of care applications, including microscopy and diagnostic testing are focused on. (via Semantic Scholar)
Source: ORCID
Added: March 21, 2019

2014 journal article

Detection and spatial mapping of mercury contamination in water samples using a smart-phone

ACS Nano, 8(2), 1121–1129.

By: Q. Wei*, R. Nagi*, K. Sadeghi*, S. Feng*, E. Yan*, S. Ki*, R. Caire*, D. Tseng*, A. Ozcan*

Contributors: Q. Wei*, R. Nagi*, K. Sadeghi*, S. Feng*, E. Yan*, S. Ki*, R. Caire*, D. Tseng*, A. Ozcan*

author keywords: smart-phone sensor; mercury detection; colorimetric sensor; gold nanoparticles; aptamers
MeSH headings : California; Cell Phone; Colorimetry; Gold / chemistry; Mercury / analysis; Metal Nanoparticles; Microcomputers; Water Pollutants, Chemical / analysis
TL;DR: A smart-phone-based hand-held platform that allows the quantification of mercury(II) ions in water samples with parts per billion (ppb) level of sensitivity is introduced and a mercury contamination map is generated by measuring water samples at over 50 locations in California (USA). (via Semantic Scholar)
Source: ORCID
Added: March 21, 2019

2014 conference paper

Fluorescent imaging over an ultra-large field-of-view of 532 cm<sup>2</sup> using a flatbed scanner

Progress in Biomedical Optics and Imaging - Proceedings of SPIE, 8951.

By: Z. Göröcs*, Y. Ling*, M. Yu*, D. Karahalios*, K. Mogharabi*, K. Lu*, Q. Wei*, A. Ozcan*

Contributors: Z. Göröcs*, Y. Ling*, M. Yu*, D. Karahalios*, K. Mogharabi*, K. Lu*, Q. Wei*, A. Ozcan*

Source: ORCID
Added: March 21, 2019

2014 journal article

Imaging and sizing of single DNA molecules on a mobile phone

ACS Nano, 8(12), 12725–12733.

By: Q. Wei*, W. Luo*, S. Chiang*, T. Kappel*, C. Mejia*, D. Tseng*, R. Chan*, E. Yan* ...

Contributors: Q. Wei*, W. Luo*, S. Chiang*, T. Kappel*, C. Mejia*, D. Tseng*, R. Chan*, E. Yan* ...

author keywords: cell phone microscopy; fluorescence imaging; DNA imaging; DNA sizing; point-of-care; mobile health
MeSH headings : Cell Phone; Cost-Benefit Analysis; DNA / chemistry; Microscopy, Fluorescence / economics; Microscopy, Fluorescence / instrumentation
TL;DR: This work has created a computational framework and a mobile phone application connected to a server back-end for measurement of the lengths of individual DNA molecules that are labeled and stretched using disposable chips. (via Semantic Scholar)
Source: ORCID
Added: March 21, 2019

2014 journal article

Research highlights: digital assays on chip

Lab on a Chip, 15(1), 17–22.

Contributors: D. Kim*, Q. Wei*, J. Kong*, A. Ozcan* & D. Di Carlo*

Source: ORCID
Added: March 21, 2019

2014 conference paper

Single Nanoparticle and Virus Detection Using a Smart Phone Based Fluorescence Microscope

Optics InfoBase Conference Papers, 2014-January. http://www.scopus.com/inward/record.url?eid=2-s2.0-84905369899&partnerID=MN8TOARS

By: Q. Wei, H. Qi, W. Luo, D. Tseng, L. Bentolila, T. Wu, R. Sun, A. Ozcan

Contributors: Q. Wei, H. Qi, W. Luo, D. Tseng, L. Bentolila, T. Wu, R. Sun, A. Ozcan

Source: ORCID
Added: March 21, 2019

2013 journal article

Fluorescent Imaging of Single Nanoparticles and Viruses on a Smart Phone

Acs Nano, 7(10), 9147–9155.

Contributors: Q. Wei*, H. Qi*, W. Luo*, D. Tseng*, S. Ki*, Z. Wan*, Z. Gorocs*, L. Bentolila* ...

MeSH headings : Cell Phone; Cytomegalovirus / isolation & purification; Microscopy, Electron, Scanning; Microscopy, Fluorescence / methods; Nanoparticles
TL;DR: A field-portable fluorescence microscopy attachment to the cell phone, weighing only ~186 g, could be used for specific and sensitive imaging of subwavelength objects including various bacteria and viruses and, therefore, could provide a valuable platform for the practice of nanotechnology in field settings and for conducting viral load measurements and other biomedical tests even in remote and resource-limited environments. (via Semantic Scholar)
Source: ORCID
Added: March 21, 2019

2013 journal article

Giga-pixel fluorescent imaging over an ultra-large field-of-view using a flatbed scanner

Lab on a Chip, 13(22), 4460–4466.

By: Z. Gorocs*, Y. Ling*, M. Yu*, D. Karahalios*, K. Mogharabi*, K. Lu*, Q. Wei*, A. Ozcan*

Contributors: Z. Gorocs*, Y. Ling*, M. Yu*, D. Karahalios*, K. Mogharabi*, K. Lu*, Q. Wei*, A. Ozcan*

MeSH headings : Fluorescent Dyes / chemistry; Humans; Image Enhancement / instrumentation; Microfluidic Analytical Techniques / instrumentation; Microscopy, Fluorescence / instrumentation; Microscopy, Fluorescence / methods; Software
TL;DR: A new fluorescent imaging technique that can screen for fluorescent micro-objects over an ultra-wide field-of-view (FOV) of ~532 cm(2), i.e., 19 cm × 28 cm, reaching a space-bandwidth product of more than 2 billion. (via Semantic Scholar)
Source: ORCID
Added: March 21, 2019

2013 conference paper

Lensfree holographic cytometry using plasmonic nanoparticles

2013 IEEE Photonics Conference, IPC 2013, 3–4.

Contributors: Q. Wei*, E. McLeod*, H. Qi*, Z. Wan*, R. Sun* & A. Ozcan*

TL;DR: Cellular contrast was demonstrated for the first time in lensfree on-chip holography by employing antibody-conjugated gold and silver nanoparticles (NPs) as contrast agents. (via Semantic Scholar)
Source: ORCID
Added: March 21, 2019

2013 journal article

On-Chip Cytometry using Plasmonic Nanoparticle Enhanced Lensfree Holography

Scientific Reports, 3.

Contributors: Q. Wei*, E. McLeod*, H. Qi*, Z. Wan*, R. Sun* & A. Ozcan*

MeSH headings : Equipment Design; Equipment Failure Analysis; Flow Cytometry / instrumentation; Holography / instrumentation; Imaging, Three-Dimensional / instrumentation; Lenses; Microscopy / instrumentation; Nanoparticles / chemistry; Surface Plasmon Resonance / instrumentation
TL;DR: The use of plasmon-resonant metallic nanoparticles are demonstrated to automatically recognize different cell types based on their plAsmon-enhanced lensfree holograms, detected and reconstructed over a large field-of-view of e.g., ~24 mm2. (via Semantic Scholar)
Source: ORCID
Added: March 21, 2019

2013 journal article

Silver nanoparticle-specific mitotoxicity in Daphnia magna

Nanotoxicology, 8(8), 833–842.

By: M. Stensberg*, R. Madangopal*, G. Yale*, Q. Wei*, H. Ochoa-Acuna*, A. Wei*, E. McLamore*, J. Rickus*, D. Porterfield*, M. Sepulveda*

Contributors: M. Stensberg*, R. Madangopal*, G. Yale*, Q. Wei*, H. Ochoa-Acuna*, A. Wei*, E. McLamore*, J. Rickus*, D. Porterfield*, M. Sepulveda*

author keywords: nanosilver; ionic silver; mechanisms of toxicity; realtime sensing Daphnia
MeSH headings : Animals; Daphnia / chemistry; Daphnia / drug effects; Daphnia / metabolism; Lethal Dose 50; Metal Nanoparticles / chemistry; Metal Nanoparticles / toxicity; Mitochondrial Membranes / drug effects; Permeability / drug effects; Protons; Silver / chemistry; Silver / pharmacokinetics; Silver / toxicity; Sodium / metabolism; Toxicity Tests
TL;DR: The abnormalities in mitochondrial activity for Ag NP-exposed organisms suggest a nanoparticle-specific MOT, distinct from that induced by Ag ions, which is proposed to produce a greater toxic response overall. (via Semantic Scholar)
Source: ORCID
Added: March 21, 2019

2012 journal article

Differential response of macrophages to core-shell Fe3O4@Au nanoparticles and nanostars

Nanoscale, 4(22), 7143–7148.

By: W. Xia*, H. Song*, Q. Wei* & A. Wei*

Contributors: W. Xia*, H. Song*, Q. Wei* & A. Wei*

MeSH headings : Animals; Cell Line; Cell Survival / drug effects; Ferrosoferric Oxide / chemistry; Gold / chemistry; Macrophages / drug effects; Metal Nanoparticles / chemistry; Metal Nanoparticles / toxicity; Mice; Particle Size; Phagocytosis / drug effects; Serum Albumin / chemistry; Serum Albumin / metabolism
TL;DR: Observations show that differences in NP size and shape are sufficient to produce diverse responses in macrophages following uptake, and MTT assays indicate a low cytotoxic response to either SCS-NPs or NSTs despite differences in Macrophage behavior. (via Semantic Scholar)
Source: ORCID
Added: March 21, 2019

2011 article

Gold Nanorods as Theranostic Agents

Wei, A., Wei, Q., & Leonov, A. P. (2011, May). Nanoplatform-Based Molecular Imaging, pp. 659–681.

By: A. Wei*, Q. Wei* & A. Leonov*

Contributors: A. Wei*, Q. Wei* & A. Leonov*

Source: ORCID
Added: March 21, 2019

2011 journal article

Metal-Mesh Lithography

Acs Applied Materials &Amp; Interfaces, 3(12), 4812–4818.

By: Z. Tang*, Q. Wei* & A. Wei*

Contributors: Z. Tang*, Q. Wei* & A. Wei*

author keywords: lithography; surface chemistry; microarrays; chemisorption; wetting
MeSH headings : Copper / chemistry; Printing / instrumentation; Printing / methods; Surface Properties
TL;DR: Metal-mesh lithography is a practical hybrid of microcontact printing and capillary force lithography that can be applied over millimeter-sized areas with a high level of uniformity, and can be reproduced with low micrometer resolution. (via Semantic Scholar)
UN Sustainable Development Goal Categories
14. Life Below Water (OpenAlex)
Source: ORCID
Added: March 21, 2019

2011 journal article

Optical Imaging with Dynamic Contrast Agents

Chemistry-a European Journal, 17(4), 1080–1091.

By: Q. Wei* & A. Wei*

Contributors: Q. Wei* & A. Wei*

author keywords: contrast agents; imaging techniques; magnetic properties; nanoparticles; photothermal reactions
MeSH headings : Contrast Media / chemistry; Diagnostic Imaging; Fluorescence; Image Processing, Computer-Assisted; Magnetics; Molecular Dynamics Simulation; Nanoparticles / chemistry
TL;DR: Dynamic contrast is an emerging topic with considerable room for development, both with respect to molecular or nanoscale probes for signal modulation, and also to methods for more efficient image processing and editing. (via Semantic Scholar)
Source: ORCID
Added: March 21, 2019

2011 journal article

Toxicological studies on silver nanoparticles: challenges and opportunities in assessment, monitoring and imaging

Nanomedicine, 6(5), 879–898.

By: M. Stensberg*, Q. Wei*, E. McLamore*, D. Porterfield*, A. Wei* & M. Sepulveda*

Contributors: M. Stensberg*, Q. Wei*, E. McLamore*, D. Porterfield*, A. Wei* & M. Sepulveda*

author keywords: cell uptake; characterization methods; imaging; physiological sensing; silver nanoparticles; toxicology
MeSH headings : Animals; Environmental Monitoring / methods; Humans; Microscopy / methods; Nanoparticles / analysis; Nanoparticles / toxicity; Silver / analysis; Silver / toxicity
TL;DR: Several proposed mechanisms of cytotoxicity based on recent research on the transport, activity and fate of Ag NPs at the cellular and organismic level are discussed, in conjunction with traditional and recently established methods of nanoparticle characterization. (via Semantic Scholar)
Source: ORCID
Added: March 21, 2019

2010 article

Cellular Interactions of Plasmon-Resonant Gold Nanorods

Wei, Q., & Wei, A. (2010, August). Organelle-Specific Pharmaceutical Nanotechnology, pp. 507–533.

By: Q. Wei* & A. Wei*

Source: ORCID
Added: March 21, 2019

2010 journal article

Gold nanorods: multifunctional agents for cancer imaging and therapy.

Methods in Molecular Biology (Clifton, N.J.), 624, 119–130.

By: A. Wei*, A. Leonov* & Q. Wei*

Contributors: A. Wei*, A. Leonov* & Q. Wei*

author keywords: Gold; biomedical imaging; nonlinear optical microscopy; photothermal effects; surface functionalization; targeting
MeSH headings : Animals; Diagnostic Imaging / methods; Folic Acid / metabolism; Gold; Mice; Nanomedicine / methods; Nanotubes / chemistry; Nanotubes / ultrastructure; Neoplasms / diagnosis; Neoplasms / therapy; Polyethylene Glycols / metabolism; Surface Properties
TL;DR: Gold nanorods are strongly absorbing at near-infrared (NIR) frequencies and can be employed as multifunctional agents for biological imaging and theragnostics, but future clinical applications will require the rigorous removal of CTAB and reliable methods of surface functionalization for cell-selective targeting and for minimizing nonspecific uptake into cells. (via Semantic Scholar)
UN Sustainable Development Goal Categories
7. Affordable and Clean Energy (OpenAlex)
Source: ORCID
Added: March 21, 2019

2010 journal article

Plasmon-Resonant Nanoparticles and Nanostars with Magnetic Cores: Synthesis and Magnetomotive Imaging

Acs Nano, 4(9), 5163–5173.

By: H. Song*, Q. Wei*, Q. Ong* & A. Wei*

Contributors: H. Song*, Q. Wei*, Q. Ong* & A. Wei*

author keywords: imaging; magnetic nanoparticles; nanomedicine; nanostars; plasmonics; synthesis
MeSH headings : Animals; Cattle; Cell Line; Gold / chemistry; Macrophages / metabolism; Magnetics; Magnetite Nanoparticles / chemistry; Mice; Microscopy, Electron, Transmission; Molecular Imaging / methods; Optical Phenomena; Protein Transport; Serum Albumin, Bovine / metabolism
TL;DR: The polarization-sensitive MM imaging modality offers the dual advantage of enhanced signal quality and reduced background signal and can be applied toward the detection of magnetomotive NSTs in heterogeneous biological samples, as illustrated by their detection inside of granular cells such as macrophages. (via Semantic Scholar)
Source: ORCID
Added: March 21, 2019

2010 chapter

Plasmon-Resonant gold nanorods as multifunctional agents for diagnostics, imaging and photothermal therapy

In Handbook of Materials for Nanomedicine (pp. 585–631).

By: Q. Wei & A. Wei

Contributors: Q. Wei & A. Wei

Source: ORCID
Added: April 10, 2020

2010 article

Signal Generation with Gold Nanoparticles: Photophysical Properties for Sensor and Imaging Applications

Wei, Q., & Wei, A. (2010, June). The Supramolecular Chemistry of Organic-Inorganic Hybrid Materials, pp. 319–349.

By: Q. Wei* & A. Wei*

Contributors: Q. Wei* & A. Wei*

Source: ORCID
Added: March 21, 2019

2010 journal article

Zwitterionic phosphorylcholine as a better ligand for gold nanorods cell uptake and selective photothermal ablation of cancer cells

Chemical Communications, 46(9), 1479–1481.

Contributors: W. Zhou*, J. Shao*, J. Qiao*, Q. Wei*, J. Tang* & J. Jian*

MeSH headings : Cell Line, Tumor; Gold / chemistry; Humans; Infrared Rays; Ligands; Metal Nanoparticles / chemistry; Microscopy, Electron, Transmission; Phosphorylcholine / chemistry
TL;DR: The conjugation of zwitterionic phosphorylcholine onto gold nanorods leads to enhanced and selective uptake within cancer cells. (via Semantic Scholar)
Source: ORCID
Added: March 21, 2019

2009 journal article

COLL 347-Polarization-sensitive NIR imaging modalities based on gold nanorods and nanostars

Abstracts of Papers of the American Chemical Society, 238. http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=ORCID&SrcApp=OrcidOrg&DestLinkType=FullRecord&DestApp=WOS_CPL&KeyUT=WOS:000207861903202&KeyUID=WOS:000207861903202

By: Q. Wei, H. Song, A. Leonov, K. Ritchie & A. Wei

Contributors: Q. Wei, H. Song, A. Leonov, K. Ritchie & A. Wei

Source: ORCID
Added: March 21, 2019

2009 journal article

Gold Nanorods as Contrast Agents for Biological Imaging: Optical Properties, Surface Conjugation and Photothermal Effects

Photochemistry and Photobiology, 85(1), 21–32.

By: L. Tong*, Q. Wei*, A. Wei* & J. Cheng*

Contributors: L. Tong*, Q. Wei*, A. Wei* & J. Cheng*

MeSH headings : Animals; Contrast Media / chemistry; Gold / chemistry; Humans; Molecular Probe Techniques; Nanotubes / chemistry; Photochemical Processes; Surface Properties
TL;DR: The combination of plasmon‐resonant optical properties, intense local photothermal effects and robust surface chemistry render gold NRs as promising theragnostic agents. (via Semantic Scholar)
UN Sustainable Development Goal Categories
7. Affordable and Clean Energy (OpenAlex)
Source: ORCID
Added: March 21, 2019

2009 journal article

Gyromagnetic Imaging: Dynamic Optical Contrast Using Gold Nanostars with Magnetic Cores

Journal of the American Chemical Society, 131(28), 9728–9734.

By: Q. Wei*, H. Song*, A. Leonov*, J. Hale*, D. Oh*, Q. Ong*, K. Ritchie*, A. Wei*

Contributors: Q. Wei*, H. Song*, A. Leonov*, J. Hale*, D. Oh*, Q. Ong*, K. Ritchie*, A. Wei*

MeSH headings : Biological Transport; Gold / chemistry; Gold / metabolism; Humans; KB Cells; Luminescence; Magnetics; Metal Nanoparticles / chemistry; Motion; Optical Phenomena; Rotation; Time Factors; Tomography, Optical Coherence
TL;DR: Gyromagnetic imaging of nanostars inside of tumor cells is demonstrated, using broadband excitation: while their time- domain signals are obscured by incoherent scattering, their Fourier-domain signals can be clearly resolved in less than a second. (via Semantic Scholar)
Source: ORCID
Added: March 21, 2019

2008 journal article

COLL 66-Gold nanostars with magnetic cores as dynamic optical imaging agents

Abstracts of Papers of the American Chemical Society, 236. http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=ORCID&SrcApp=OrcidOrg&DestLinkType=FullRecord&DestApp=WOS_CPL&KeyUT=WOS:000270256303742&KeyUID=WOS:000270256303742

By: Q. Wei, H. Song, Q. Ong, K. Ritchie & A. Wei

Contributors: Q. Wei, H. Song, Q. Ong, K. Ritchie & A. Wei

Source: ORCID
Added: March 21, 2019

2008 conference paper

NIR responsive core/shell nanohybrids for smart drug delivery

8th World Biomaterials Congress 2008, 1, 297. http://www.scopus.com/inward/record.url?eid=2-s2.0-84869034553&partnerID=MN8TOARS

By: Q. Wei, J. Ji & J. Shen

Contributors: Q. Wei, J. Ji & J. Shen

Source: ORCID
Added: April 10, 2020

2008 journal article

Synthesis of near-infrared responsive gold nanorod/PNIPAAm core/shell nanohybrids via surface initiated ATRP for smart drug delivery

Macromolecular Rapid Communications, 29(8), 645–650.

By: Q. Wei*, J. Ji* & J. Shen*

Contributors: Q. Wei*, J. Ji* & J. Shen*

author keywords: drug delivery systems; gold nanorods; nanocomposites; near infrared responsive; stimuli-sensitive polymers
TL;DR: Norvancomycin (NVan) loading and release experiments show that the drug release rate can be modulated by NIR irradiation, and such an intelligent drug-delivery system might find applications in non-invasive controlled drug release in future. (via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being (OpenAlex)
Source: ORCID
Added: March 21, 2019

2008 journal article

The Escherichia coli O157 : H7 DNA detection on a gold nanoparticle-enhanced piezoelectric biosensor

Chinese Science Bulletin, 53(8), 1175–1184.

By: L. Wang*, Q. Wei*, C. Wu*, Z. Hu*, J. Ji* & P. Wang*

Contributors: L. Wang*, Q. Wei*, C. Wu*, Z. Hu*, J. Ji* & P. Wang*

author keywords: piezoelectric biosensor; gold nanoparticle; E. coli O157 : H7
Source: ORCID
Added: March 21, 2019

2008 journal article

Thermosensitive Nanocables Prepared by Surface-Initiated Atom Transfer Radical Polymerization

Nanoscale Research Letters, 4(1), 84–89.

By: Q. Wei*, W. Zhou*, J. Ji* & J. Shen*

Contributors: Q. Wei*, W. Zhou*, J. Ji* & J. Shen*

author keywords: Nanocables; Gold nanowires; Poly(N-isopropylacrylamide); Surface-initiated atom transfer radical polymerization Thermoresponsive
TL;DR: Smart nanocables consisting of Au nanowire cores and poly(N-isopropylacrylamide) sheaths (denoted as Au/PNIPAAm) show immense potentials as building blocks for novel thermosensitive nanodevices in future. (via Semantic Scholar)
Source: ORCID
Added: March 21, 2019

2008 journal article

Two-Photon Luminescence Imaging of Bacillus Spores Using Peptide-Functionalized Gold Nanorods

Nano Research, 1(6), 450–456.

By: W. He*, W. Henne*, Q. Wei*, Y. Zhao*, D. Doorneweerd*, J. Cheng*, P. Low*, A. Wei*

Contributors: W. He*, W. Henne*, Q. Wei*, Y. Zhao*, D. Doorneweerd*, J. Cheng*, P. Low*, A. Wei*

author keywords: Bionanotechnology; nanorods; nonlinear optics; pathogen detection; peptides
TL;DR: It was determined that gold nanorods functionalized with a cysteine-terminated homing peptide are capable of significant TPL output even when irradiated at near infrared wavelengths far from their longitudinal plasmon resonance (LPR), permitting considerable flexibility in the choice of GNR aspect ratio or excitation wavelength for TPL imaging. (via Semantic Scholar)
Source: ORCID
Added: March 21, 2019

2008 journal article

pH Controlled Synthesis of High Aspect-Ratio Gold Nanorods

Journal of Nanoscience and Nanotechnology, 8(11), 5708–5714.

By: Q. Wei*, J. Ji & J. Shen

Contributors: Q. Wei*, J. Ji & J. Shen

author keywords: Gold Nanorods; High Aspect-Ratio; pH Control Synthesis; Seed Mediated; Slow Growth
TL;DR: The two-step synthesis of high aspect-ratio gold nanorods via combining low pH growth and higher cetyltrimethylammonium bromide concentration is reported, which could be excellent building blocks for optical or electrical nanodevices in future. (via Semantic Scholar)
Source: ORCID
Added: March 21, 2019

2007 conference paper

A QCM biosensor based on gold nanoparticles amplification for real-time bacteria DNA detection

Proceedings of the 2007 International Conference on Information Acquisition, ICIA, 46–51.

Contributors: L. Wang*, Q. Wei*, C. Wu*, J. Ji* & P. Wang*

TL;DR: A DNA piezoelectric biosensing method for real-time detection of E. coli O157:H7 in a circulating-flow system and it is practical to develop a sensitive and effective QCM biosensor for pathogenic bacteria detection based on specific DNA analysis. (via Semantic Scholar)
Source: ORCID
Added: March 21, 2019

2007 conference paper

Detection of E. coli O157:H7 DNA by a novel QCM biosensor coupled with gold nanoparticles amplification

2007 7th IEEE International Conference on Nanotechnology - IEEE-NANO 2007, Proceedings, 330–333.

TL;DR: A sensitive and effective QCM biosensor for pathogenic bacteria detection based on specific DNA analysis by means of nanogold modification and mass amplification for Escherichia coli O157:H7 DNA detection was explored and developed. (via Semantic Scholar)
Source: ORCID
Added: March 21, 2019

2007 journal article

Norvancomycin-capped silver nanoparticles: Synthesis and antibacterial activities against E-coli

Science in China Series B-Chemistry, 50(3), 418–424.

By: Q. Wei*, J. Ji*, J. Fu* & J. Shen*

Contributors: Q. Wei*, J. Ji*, J. Fu* & J. Shen*

author keywords: silver nanoparticle; norvancomycin; Gram-negative bacterial strain; bioconjugate
TL;DR: It is proposed that silver nanoparticles may help destroy the stability of the outer membrane of E. coli, which makes NVan easier to bind to the nether part of the peptidoglycan structure and enables Ag@NVan to be an effective inhibitor of GNB. (via Semantic Scholar)
Source: ORCID
Added: March 21, 2019

Employment

Updated: May 2nd, 2017 22:51

2017 - present

North Carolina State University Raleigh, NC, US
Chemical and Biomolecular Engineering

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