Works (5)

Updated: November 7th, 2023 05:00

2023 article

Identification and characterization of CHO host-cell proteins in monoclonal antibody bioprocessing

Oh, Y. H., Mendola, K. M., Choe, L. H., Min, L., Lavoie, A. R., Sripada, S. A., … Lenhoff, A. M. (2023, October 25). BIOTECHNOLOGY AND BIOENGINEERING, Vol. 10.

By: Y. Oh*, K. Mendola*, L. Choe*, L. Min*, A. Lavoie n, S. Sripada n, T. Williams n, K. Lee* ...

author keywords: bioprocessing; Chinese hamster ovary cells; host-cell proteins; monoclonal antibodies; purification
UN Sustainable Development Goal Categories
3. Good Health and Well-being (OpenAlex)
Sources: Web Of Science, NC State University Libraries
Added: November 6, 2023

2020 review

Past, Present, and Future of Affinity-based Cell Separation Technologies

[Review of ]. ACTA BIOMATERIALIA, 112, 29–51.

By: K. Bacon n, A. Lavoie n, B. Rao n, M. Daniele n & S. Menegatti n

author keywords: Cell purification; Immunoaffinity; MACS; FACS; Microfluidics
MeSH headings : Cell Separation; Chromatography, Affinity; Flow Cytometry; Ligands; Regenerative Medicine
TL;DR: This review discusses past and current methods for developing cell-targeting affinity ligands and their application in cell purification, along with the benefits and challenges associated with different purification formats, and presents new technologies towards improving the viability and throughput of cell products for tissue engineering and regenerative medicine. (via Semantic Scholar)
UN Sustainable Development Goal Categories
9. Industry, Innovation and Infrastructure (OpenAlex)
Sources: Web Of Science, NC State University Libraries
Added: August 3, 2020

2019 journal article

Affordable Microfluidic Bead-Sorting Platform for Automated Selection of Porous Particles Functionalized with Bioactive Compounds


By: S. Saberi-Bosari n, M. Omary n, A. Lavoie n, R. Prodromou n, K. Day n, S. Menegatti n, A. San-Miguel n

MeSH headings : Combinatorial Chemistry Techniques / instrumentation; Humans; Image Processing, Computer-Assisted; Immunoglobulin G / chemistry; Lab-On-A-Chip Devices; Microfluidic Analytical Techniques / instrumentation; Peptide Library; Porosity; Proof of Concept Study; Unsupervised Machine Learning
TL;DR: A low-cost microfluidics-based platform enabling automated screening of small porous beads from solid-phase peptide libraries with high sensitivity and specificity, to identify leads with high binding affinity for a biological target is presented. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries, ORCID
Added: May 28, 2019

2019 journal article

Discovery and Evaluation of Peptide Ligands for Selective Adsorption and Release of Cas9 Nuclease on Solid Substrates

BIOCONJUGATE CHEMISTRY, 30(12), 3057–3068.

By: K. Day n, R. Prodromou n, S. Bosari n, A. Lavoie n, M. Omary n, C. Market n, A. San Miguel n, S. Menegatti n

MeSH headings : Adsorption; Amino Acid Sequence; Binding Sites; CRISPR-Associated Protein 9 / isolation & purification; CRISPR-Associated Protein 9 / metabolism; Drug Discovery; Ligands; Molecular Docking Simulation; Peptide Library; Peptides / metabolism; Resins, Synthetic / chemistry; Substrate Specificity
TL;DR: This work represents the first effort at developing short synthetic Cas9-binding peptides and demonstrates their applicability as affinity ligands for the purification of a Cas nuclease. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Sources: Web Of Science, NC State University Libraries, ORCID
Added: January 13, 2020

2019 journal article

Targeted Capture of Chinese Hamster Ovary Host Cell Proteins: Peptide Ligand Discovery


By: R. Lavoie n, A. Fazio n, R. Blackburn n, M. Goshe n, R. Carbonell n & S. Menegatti n

author keywords: therapeutic antibodies; host cell proteins; protein purification; peptide ligands
MeSH headings : Animals; CHO Cells; Chromatography, Affinity; Chromatography, Liquid; Cricetinae; Cricetulus; Humans; Peptides / chemistry; Peptides / isolation & purification; Proteomics / methods
TL;DR: Development of synthetic peptide ligands capable of capturing a broad spectrum of Chinese hamster ovary (CHO) HCPs with a combination of peptide species that allow for advanced mixed-mode binding is described. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: May 6, 2019

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