Works (60)

Updated: April 3rd, 2024 20:44

2023 journal article

Cytoplasmic male sterility and abortive seed traits generated through mitochondrial genome editing coupled with allotopic expression of atp1 in tobacco

FRONTIERS IN PLANT SCIENCE, 14.

By: R. Dewey n, D. Selote n, H. Griffin n, A. Dickey n, D. Jantz*, J. Smith*, A. Matthiadis*, J. Strable n, C. Kestell n, W. Smith n

author keywords: hybrid seed systems; allotopic expression; stenospermocarpy; atp1; transgene containment; substoichiometric shifting; mitoarcus; custom-designed meganucleases
TL;DR: Should the results reported here in tobacco prove to be translatable to other crop species, then multiple applications of allotopic expression of an essential mitochondrial gene followed by its elimination through genome editing can be envisaged. (via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being (OpenAlex)
13. Climate Action (Web of Science)
Source: Web Of Science
Added: October 16, 2023

2022 journal article

CLCNt2 Mediates Nitrate Content in Tobacco Leaf, Impacting the Production of Tobacco-Specific Nitrosamines in Cured Leaves

FRONTIERS IN PLANT SCIENCE, 13.

By: L. Bovet*, P. Campanoni*, J. Lu n, A. Hilfiker*, S. Kleinhans*, H. Laparra*, J. Schwaar*, R. Lewis n ...

author keywords: CLCNt2; chloride channel; tobacco; NNN; NNK; nitrate transport; chloride; proton antiporters; tobacco-specific nitrosamines
TL;DR: This study provides a straight path toward the development of new commercial tobacco varieties with reduced TSNA levels by breeding of variants deficient in active CLCNt2 copies byressing expression or knocking-out CLCnt2 has a direct impact on leaf nitrate and TSNA reduction in cured leaves without altering biomass. (via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger (Web of Science)
3. Good Health and Well-being (OpenAlex)
Source: Web Of Science
Added: March 14, 2022

2022 journal article

Knockout of a key gene of the nicotine biosynthetic pathway severely affects tobacco growth under field, but not greenhouse conditions

BMC RESEARCH NOTES, 15(1).

By: W. Smith n, Y. Matsuba n & R. Dewey n

MeSH headings : Alkaloids; Biosynthetic Pathways / genetics; Gene Expression Regulation, Plant; Nicotine; Plant Proteins / genetics; Plant Proteins / metabolism; Plants, Genetically Modified / genetics; Tobacco / genetics; Tobacco / metabolism
TL;DR: Two conclusions can be inferred from this work: (1) QPT1 gene function alone appears to be inadequate for meeting the QPT demands of the plant for primary metabolism when grown in a field environment; and (2) the complete knockout of QPT2 function is not a viable strategy for producing agronomically useful, low nicotine tobaccos. (via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger (Web of Science)
3. Good Health and Well-being (OpenAlex)
13. Climate Action (Web of Science)
Source: Web Of Science
Added: September 19, 2022

2021 journal article

Constitutive activation of nitrate reductase in tobacco alters flowering time and plant biomass

SCIENTIFIC REPORTS, 11(1).

By: J. Lu n, N. Chandrakanth n, R. Lewis n, K. Andres n, L. Bovet*, S. Goepfert*, R. Dewey n

MeSH headings : Biomass; Environment; Flowers / genetics; Gene Expression Regulation, Plant; Gene-Environment Interaction; Genetic Association Studies; Nitrate Reductase / genetics; Nitrate Reductase / metabolism; Organ Specificity / genetics; Plant Development / genetics; Tobacco / physiology; Transcriptional Activation
TL;DR: It is shown that plants expressing a constitutively active NR construct, designated 35S:S523D-NR, display an early-flowering phenotype that is also associated with a substantial reduction in plant biomass, and the mechanism by which yield was restored differed markedly between the two crosses. (via Semantic Scholar)
Source: Web Of Science
Added: March 22, 2021

2021 journal article

Discovery of a naturally-occurring allele of eIF4E1.S in Nicotiana tabacum and development of a co-dominant marker

EUPHYTICA, 217(7).

By: S. Lin*, R. Dewey n, R. Wang*, J. Yu*, M. Long*, J. Zhang*, S. Yu*, J. Zhang*, Z. Wang*, X. Ren*

author keywords: Nicotiana tabacum; Potato virus Y; Naturally-occurring allele; S; Co-dominant marker
TL;DR: A novel source of resistance and an ideal co-dominant marker that can be applied in breeding for resistance to PVY and other potyviruses are described. (via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger (Web of Science)
3. Good Health and Well-being (OpenAlex)
13. Climate Action (Web of Science)
Source: Web Of Science
Added: September 7, 2021

2021 article

Discovery of a novel eIF4E1.S allele conferring PVY resistance in Chinese tobacco (Nicotiana tabacum) landraces

Lin, S., Dewey, R. E., Wang, R., Ren, X., Wang, Z., Zhang, J., & Long, M. (2021, June 22). PLANT BREEDING.

By: S. Lin*, R. Dewey n, R. Wang*, X. Ren*, Z. Wang*, J. Zhang*, M. Long*

author keywords: co-dominant marker; S; naturally-occurring allele; Nicotiana tabacum; potato virus Y; susceptibility gene
TL;DR: The results described here provide valuable genetic insights into Chinese tobacco landraces as a source of naturally occurring PVY resistance, as a powerful tool for the molecular marker- assisted breeding of tobacco varieties containing the resistance source eIF4E1. (via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger (Web of Science)
3. Good Health and Well-being (OpenAlex)
13. Climate Action (Web of Science)
Source: Web Of Science
Added: July 6, 2021

2020 journal article

An Efficient Stevia rebaudiana Transformation System and In vitro Enzyme Assays Reveal Novel Insights into UGT76G1 Function

SCIENTIFIC REPORTS, 10(1).

By: Q. Wu n, C. La Hovary n, H. Chen n, X. Li n, H. Eng n, V. Vallejo*, R. Qu n, R. Dewey n

MeSH headings : Alternative Splicing; Glycosyltransferases / biosynthesis; Glycosyltransferases / genetics; Isoenzymes / biosynthesis; Isoenzymes / genetics; Plant Proteins / biosynthesis; Plant Proteins / genetics; Plants, Genetically Modified / enzymology; Plants, Genetically Modified / genetics; Stevia / enzymology; Stevia / genetics; Transformation, Genetic
TL;DR: A highly efficient Agrobacterium-mediated stable transformation system using axillary shoots as the initial explant is reported and evidence suggesting that alternative and/or aberrant splicing may serve to influence the ability of the plant to produce functional UGT76G1 transcripts, and possibly produce enzyme variants within the plant is found. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: September 14, 2020

2020 journal article

Genetic and Agronomic Analysis of Tobacco Genotypes Exhibiting Reduced Nicotine Accumulation Due to Induced Mutations in Berberine Bridge Like (BBL) Genes

FRONTIERS IN PLANT SCIENCE, 11.

By: R. Lewis n, K. Drake-Stowe n, C. Heim n, T. Steede n, W. Smith n & R. Dewey n

author keywords: tobacco; Nicotiana tabacum; nicotine; alkaloids; gene editing; regulation
TL;DR: Genetic methodologies for reducing nicotine accumulation in the tobacco plant (Nicotiana tabacum L.) are of interest because of potential future regulations that could mandate lowering of this alkaloid in conventional cigarettes. (via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger (Web of Science)
3. Good Health and Well-being (OpenAlex)
13. Climate Action (Web of Science)
Source: Web Of Science
Added: May 18, 2020

2018 journal article

Characterization of Nicotiana tabacum genotypes possessing deletion mutations that affect potyvirus resistance and the production of trichome exudates

BMC GENOMICS, 19.

By: K. Dluge n, Z. Song*, B. Wang*, W. Steede n, B. Xiao*, Y. Liu*, R. Dewey n

author keywords: Nicotiana tabacum; VAM; Va; Trichome exudate
MeSH headings : Chromosomes, Human, Pair 21 / genetics; Exudates and Transudates; Gene Expression Regulation, Plant / genetics; Genotype; Humans; Plant Proteins / genetics; Plants, Genetically Modified / genetics; Potyvirus / genetics; Tobacco / genetics; Trichomes / genetics
TL;DR: The feasibility of using RNA-seq and emerging whole genome sequence resources in tobacco to characterize the VAM and va deletion mutants is demonstrated and the discovery of genes underlying some of the phenotypic traits associated with these historically important loci are led to. (via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger (Web of Science)
3. Good Health and Well-being (OpenAlex)
13. Climate Action (Web of Science)
Source: Web Of Science
Added: August 6, 2018

2016 journal article

Expression of a constitutively active nitrate reductase variant in tobacco reduces tobacco-specific nitrosamine accumulation in cured leaves and cigarette smoke

PLANT BIOTECHNOLOGY JOURNAL, 14(7), 1500–1510.

By: J. Lu n, L. Zhang n, R. Lewis n, L. Bovet*, S. Goepfert*, A. Jack*, J. Crutchfield*, H. Ji*, R. Dewey n

author keywords: nitrate reductase; tobacco; tobacco-specific nitrosamines; burley; nitrogen-assimilation pathway; NNN; NNK
MeSH headings : Carcinogens / metabolism; Metabolic Networks and Pathways; Nitrate Reductase / genetics; Nitrates / metabolism; Nitrogen / metabolism; Nitrosamines / metabolism; Plant Leaves / genetics; Plant Leaves / metabolism; Plants, Genetically Modified / metabolism; Tobacco / genetics; Tobacco / metabolism; Tobacco Products
TL;DR: Investigation of the potential of molecular genetic strategies to lower nitrate levels in burley tobaccos by overexpressing genes encoding key enzymes of the nitrogen‐assimilation pathway found only the expression of a constitutively active nitrate reductase (NR) dramatically decreased free nitrates levels in the leaves. (via Semantic Scholar)
Source: Web Of Science
Added: August 6, 2018

2015 journal article

Overexpression of ubiquitin-like LpHUB1 gene confers drought tolerance in perennial ryegrass

PLANT BIOTECHNOLOGY JOURNAL, 13(5), 689–699.

By: M. Patel n, S. Milla-Lewis n, W. Zhang n, K. Templeton*, W. Reynolds n, K. Richardson*, M. Biswas*, M. Zuleta n ...

author keywords: drought tolerance; HUB1; Lolium perenne; small ORF; transgenic
MeSH headings : Base Sequence; Chlorophyll / metabolism; Droughts; Gene Expression; Gene Expression Regulation, Plant; Lolium / genetics; Lolium / physiology; Molecular Sequence Data; Plant Leaves / genetics; Plant Leaves / physiology; Plant Proteins / genetics; Plant Proteins / metabolism; Plants, Genetically Modified; Sequence Analysis, DNA; Stress, Physiological; Ubiquitin / genetics; Ubiquitin / metabolism; Water / physiology
TL;DR: Improved drought tolerance in pasture-type and turf-type transgenic perennial ryegrass plants overexpressing LpHUB1 showed improved drought tolerance, as evidenced by improved turf quality, maintenance of turgor and increased growth. (via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger (Web of Science)
13. Climate Action (Web of Science)
Sources: Web Of Science, ORCID, NC State University Libraries
Added: August 6, 2018

2015 journal article

Transgenic and Mutation-Based Suppression of a Berberine Bridge Enzyme-Like (BBL) Gene Family Reduces Alkaloid Content in Field-Grown Tobacco

PLOS ONE, 10(2).

By: R. Lewis n, H. Lopez n, S. Bowen n, K. Andres n, W. Steede n & R. Dewey n

MeSH headings : Alkaloids / biosynthesis; Alkaloids / chemistry; Gene Expression Regulation, Plant; Mutation; Plant Proteins / antagonists & inhibitors; Plant Proteins / genetics; Plant Proteins / metabolism; Plants, Genetically Modified / genetics; Plants, Genetically Modified / metabolism; RNA Interference; Tobacco / genetics; Tobacco / growth & development; Tobacco / metabolism
TL;DR: Transgenic doubled haploid lines of tobacco cultivar K326 carrying an RNAi construct designed to reduce expression of the BBL gene family were produced, indicating that a range of alkaloid phenotypes could be produced, with the triple homozygous knockout genotype exhibiting greater than a 13-fold reduction in percent total alkaloids. (via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger (Web of Science)
3. Good Health and Well-being (OpenAlex)
13. Climate Action (Web of Science)
Source: Web Of Science
Added: August 6, 2018

2014 journal article

Mapping the low palmitate fap1 mutation and validation of its effects in soybean oil and agronomic traits in three soybean populations

THEORETICAL AND APPLIED GENETICS, 127(1), 97–111.

By: A. Cardinal n, R. Whetten n, S. Wang n, J. Auclair, D. Hyten*, P. Cregan*, E. Bachlava*, J. Gillman* ...

MeSH headings : Blotting, Northern; Chromosome Mapping; Chromosomes, Plant; Genetic Association Studies; Palmitates / metabolism; Plant Proteins / genetics; Quantitative Trait Loci; Soybean Oil / chemistry; Soybeans / genetics; Soybeans / metabolism
TL;DR: The fap1 phenotype is caused by a single unique SNP in the GmKASIIIA gene, and two major QTL for 16:0 content located in chromosome 5 (GmFATB1a, fapnc) and chromosome 9 near BARCSOYSSR_09_1707 that explained, with their interaction, 66–94 % of the variation in 16: 0 content in the three populations. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Source: Web Of Science
Added: August 6, 2018

2013 journal article

(R)-nicotine biosynthesis, metabolism and translocation in tobacco as determined by nicotine demethylase mutants

PHYTOCHEMISTRY, 95, 188–196.

By: B. Cai*, A. Jack*, R. Lewis n, R. Dewey n & L. Bush*

author keywords: Nicotiana tabacum L.; Tobacco; Solanaceae; (R)-nicotine; Nornicotine; Enantioselectivity; Nicotine demethylase
MeSH headings : Biological Transport; Cytochrome P-450 Enzyme System / metabolism; Methylation; Mutation; Nicotine / analogs & derivatives; Nicotine / biosynthesis; Nicotine / metabolism; Plant Leaves / metabolism; Plant Proteins / metabolism; Plant Roots / metabolism; Tobacco / enzymology; Tobacco / metabolism
TL;DR: The results confirmed the enantioselectivity of three nicotine demethylases in planta, could be used to predict the changes of nicotine and nornicotine composition, and may facilitate demethylase discovery in the future. (via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being (Web of Science; OpenAlex)
Source: Web Of Science
Added: August 6, 2018

2013 journal article

Enhanced Agrobacterium-mediated transformation efficiencies in monocot cells is associated with attenuated defense responses

PLANT MOLECULAR BIOLOGY, 81(3), 273–286.

By: W. Zhang n, R. Dewey n, W. Boss n, B. Phillippy n & R. Qu n

author keywords: Cold shock; Glutamine; myo-inositol; Plant defense response; Transformation
MeSH headings : Agrobacterium tumefaciens / genetics; Agrobacterium tumefaciens / physiology; Arabidopsis / drug effects; Arabidopsis / genetics; Arabidopsis / immunology; Arabidopsis / physiology; Cell Wall / metabolism; Cold Temperature; Culture Media; Gene Transfer Techniques; Genetic Vectors; Glutamine / pharmacology; Green Fluorescent Proteins; Hydrogen Peroxide / metabolism; Inositol / pharmacology; Lignin / metabolism; Lolium / drug effects; Lolium / genetics; Lolium / immunology; Lolium / physiology; Oryza / drug effects; Oryza / genetics; Oryza / immunology; Oryza / physiology; Phytic Acid / metabolism; Plant Immunity; Plant Roots / drug effects; Plant Roots / genetics; Plant Roots / immunology; Plant Roots / physiology; Plants, Genetically Modified; Regeneration; Seeds / drug effects; Seeds / genetics; Seeds / immunology; Seeds / physiology; Starch / metabolism; Tissue Culture Techniques; Transformation, Genetic
TL;DR: The utilization of culture conditions associated with an attenuation of defense responses in monocot plant cells led to highly improved Agrobacterium-mediated transformation efficiencies in perennial ryegrass (Lolium perenne L.). (via Semantic Scholar)
UN Sustainable Development Goal Categories
15. Life on Land (Web of Science)
Source: Web Of Science
Added: August 6, 2018

2013 journal article

Enhancing Agrobacterium tumefaciens-mediated transformation efficiency of perennial ryegrass and rice using heat and high maltose treatments during bacterial infection

PLANT CELL TISSUE AND ORGAN CULTURE, 114(1), 19–29.

By: M. Patel n, R. Dewey n & R. Qu n

author keywords: Heat shock; Maltose; Rice; Perennial ryegrass; Transformation
UN Sustainable Development Goal Categories
15. Life on Land (Web of Science)
Source: Web Of Science
Added: August 6, 2018

2013 review

Molecular genetics of alkaloid biosynthesis in Nicotiana tabacum

[Review of ]. PHYTOCHEMISTRY, 94, 10–27.

By: R. Dewey n & J. Xie*

author keywords: Nicotiana tabacum; Solanaceae; Nicotine; Nornicotine; Tobacco-specific nitrosamines; Alkaloid biosynthetic genes
MeSH headings : Alkaloids / biosynthesis; Alkaloids / chemistry; Biosynthetic Pathways / genetics; Gene Expression Regulation, Plant; Methyltransferases / genetics; Methyltransferases / metabolism; Molecular Structure; Nicotine / biosynthesis; Nicotine / chemistry; Nitrosamines / metabolism; Plant Proteins / genetics; Plant Proteins / metabolism; Pyridines / chemistry; Tobacco / enzymology; Tobacco / genetics; Tobacco / metabolism
TL;DR: Recent advances in understanding of the molecular genetics of alkaloid biosynthesis in tobacco are summarized, and the potential for applying information accrued from these studies toward efforts designed to help mitigate some of the negative health consequences associated with the use of tobacco products is discussed. (via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger (Web of Science)
3. Good Health and Well-being (OpenAlex)
13. Climate Action (Web of Science)
Source: Web Of Science
Added: August 6, 2018

2012 journal article

Development of CAPS and dCAPS markers for CYP82E4, CYP82E5v2 and CYP82E10 gene mutants reducing nicotine to nornicotine conversion in tobacco

MOLECULAR BREEDING, 29(3), 589–599.

By: D. Li*, R. Lewis n, A. Jack*, R. Dewey n, S. Bowen n & R. Miller*

author keywords: CAPS markers; dCAPS markers; Nicotiana tabacum; Nicotine conversion; Tobacco harm reduction; Mutation breeding
TL;DR: Efficient, user-friendly DNA markers to identify mutations based on single nucleotide polymorphisms (SNPs) can be used in marker-assisted selection programs to quickly introgress the desired mutations into commercial varieties in order to reduce nornicotine levels in tobacco leaves. (via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being (Web of Science; OpenAlex)
Source: Web Of Science
Added: August 6, 2018

2012 journal article

Enantioselective Demethylation of Nicotine as a Mechanism for Variable Nornicotine Composition in Tobacco Leaf

JOURNAL OF BIOLOGICAL CHEMISTRY, 287(51), 42804–42811.

By: B. Cai*, B. Siminszky*, J. Chappell*, R. Dewey n & L. Bush*

MeSH headings : Alkaloids / biosynthesis; Alkaloids / chemistry; Biosynthetic Pathways; Cytochrome P-450 Enzyme System / metabolism; Kinetics; Methylation; Models, Biological; Nicotine / analogs & derivatives; Nicotine / chemistry; Nicotine / metabolism; Plant Leaves / enzymology; Plant Leaves / metabolism; Plant Proteins / metabolism; Stereoisomerism; Substrate Specificity; Tobacco / enzymology; Tobacco / metabolism
TL;DR: The studies suggest that an enantioselective mechanism facilitates the maintenance of a reduced (R)-nicotine pool and, depending on the relative abundances of the three nicotine demethylase enzymes, can confer a high (R-enantiomer percentage within the nornicotine fraction of the leaf. (via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being (Web of Science; OpenAlex)
Source: Web Of Science
Added: August 6, 2018

2011 journal article

Molecular Analysis of GmFAD3A in Two Soybean Populations Segregating for the fan, fap1, and fap(nc) Loci

CROP SCIENCE, 51(5), 2104–2112.

By: A. Cardinal n, J. Burton*, A. Camacho-Roger n, R. Whetten n, A. Chappell*, K. Bilyeu*, J. Auclair n, R. Dewey n

TL;DR: A reduction in the steady-state mRNA levels of the GmFAD3A leads to a reduction in 18:3 synthesis within the developing seed in plants containing the fan(PI 123440) allele. (via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger (OpenAlex)
Source: Web Of Science
Added: August 6, 2018

2011 journal article

Molecular Analysis of Mutant Alleles for Elevated Palmitate Concentration in Soybean

CROP SCIENCE, 51(6), 2554–2560.

By: B. De Vries*, W. Fehr*, G. Welke* & R. Dewey n

TL;DR: The objective of this study was to determine whether the elevated palmitate phenotypes in any of these lines were associated with mutations in either of the two known 3-ketoacyl-ACP synthase II (KAS II) genes of soybean, GmKas IIA and GmkAS IIB. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Source: Web Of Science
Added: August 6, 2018

2011 journal article

Molecular Characterization of the Mutant fap3(A22) Allele for Reduced Palmitate Concentration in Soybean

CROP SCIENCE, 51(4), 1611–1616.

By: B. De Vries*, W. Fehr*, G. Welke* & R. Dewey n

TL;DR: The polymorphism in the GmFATB1a gene was used to develop a functional, codominant marker that could be used to distinguish the Fap3 and fap3(A22) alleles in segregating populations and will be useful for breeders who are developing low-saturate cultivars with the fap 3(A 22) allele. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Source: Web Of Science
Added: August 6, 2018

2010 journal article

Three nicotine demethylase genes mediate nornicotine biosynthesis in Nicotiana tabacum L Functional characterization of the CYP82E10 gene

PHYTOCHEMISTRY, 71(17-18), 1988–1998.

By: R. Lewis n, S. Bowen n, M. Keogh n & R. Dewey n

author keywords: Nicotiana tabacum; Solanaceae; Gene function; Alkaloids; Nornicotine; Tobacco specific nitrosamine (TSNA); Cytochrome P450; CYP82E10
MeSH headings : Carcinogens / chemistry; Carcinogens / pharmacology; Cytochrome P-450 Enzyme System / genetics; Cytochrome P-450 Enzyme System / metabolism; Molecular Structure; Nicotine / analogs & derivatives; Nicotine / biosynthesis; Nicotine / chemistry; Nicotine / genetics; Nicotine / metabolism; Nitrosamines / chemistry; Nitrosamines / pharmacology; Plant Leaves / chemistry; Plant Leaves / genetics; Plant Leaves / metabolism; Plant Proteins / genetics; Plant Proteins / metabolism; Tobacco / chemistry; Tobacco / enzymology; Tobacco / genetics
TL;DR: The introduction of knockout mutations in all three tobacco NND genes into commercial breeding lines promises to be a viable strategy for reducing the levels of one of the best characterized animal carcinogens found in tobacco products. (via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being (Web of Science; OpenAlex)
Source: Web Of Science
Added: August 6, 2018

2009 journal article

Functional Characterization of Phospholipid N-Methyltransferases from Arabidopsis and Soybean

JOURNAL OF BIOLOGICAL CHEMISTRY, 284(23), 15439–15447.

By: M. Keogh n, P. Courtney n, A. Kinney* & R. Dewey n

MeSH headings : Arabidopsis / enzymology; Arabidopsis / genetics; Arabidopsis Proteins / metabolism; Cloning, Molecular; DNA Primers; DNA, Complementary / genetics; DNA, Plant / genetics; Kinetics; Mutagenesis, Insertional; Phosphatidylcholines / biosynthesis; Phosphatidylethanolamine N-Methyltransferase / genetics; Phosphatidylethanolamine N-Methyltransferase / metabolism; Plant Proteins / metabolism; Polymerase Chain Reaction; Recombinant Proteins / metabolism; Saccharomyces cerevisiae / enzymology; Soybeans / enzymology; Soybeans / genetics
TL;DR: Using a heterologous yeast expression system, it was shown that plant PLMTs methylate phosphatidylmonomethylethanolamine and phosphatidyldimethyleanolamine but cannot utilize phosphatido-phosphate as a substrate, suggesting the degree with which PtdCho synthesis is dependent upon PLMT activities will also vary widely throughout the plant kingdom. (via Semantic Scholar)
Source: Web Of Science
Added: August 6, 2018

2009 journal article

Mapping and Comparison of Quantitative Trait Loci for Oleic Acid Seed Content in Two Segregating Soybean Populations

CROP SCIENCE, 49(2), 433–442.

By: E. Bachlava n, R. Dewey n, J. Burton n & A. Cardinal n

TL;DR: A whole-genome scan approach was used to identify oleate quantitative trait loci (QTLs) in a soybean population segregating for oleic acid content and a cognate segregating population to con- fi rm oleated QTLs, finding an oleates QTL with moderate effects with mod- erate effects on linkage group F. (via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger (Web of Science; OpenAlex)
13. Climate Action (Web of Science)
15. Life on Land (Web of Science)
Source: Web Of Science
Added: August 6, 2018

2009 journal article

Mapping candidate genes for oleate biosynthesis and their association with unsaturated fatty acid seed content in soybean

MOLECULAR BREEDING, 23(2), 337–347.

By: E. Bachlava n, R. Dewey n, J. Burton n & A. Cardinal n

author keywords: Soybean; Oleate biosynthesis; QTL mapping; Fatty acid content
TL;DR: Several candidate genes implicated in Oleate biosynthesis were mapped and their cosegregation with oleate, linoleate and linolenate quantitative trait loci (QTLs) was investigated, suggesting only one functional copy of the FAD6 gene, encoding a plastid localized ω-6 desaturase, exists in the soybean genome. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Source: Web Of Science
Added: August 6, 2018

2008 journal article

CYP82E4-mediated nicotine to nornicotine conversion in tobacco is regulated by a senescence-specific signaling pathway

PLANT MOLECULAR BIOLOGY, 66(4), 415–427.

By: M. Chakrabarti*, S. Bowen n, N. Coleman*, K. Meekins*, R. Dewey n & B. Siminszky*

author keywords: alkaloid; cytochrome P450; SAG; senescence-associated gene; stress; transcriptional regulation
MeSH headings : Aging / genetics; Cytochrome P-450 Enzyme System / genetics; Cytochrome P-450 Enzyme System / metabolism; Ethylenes / pharmacology; Gene Expression Regulation, Enzymologic; Gene Expression Regulation, Plant; Glucuronidase / genetics; Glucuronidase / metabolism; Plant Leaves / enzymology; Plant Leaves / growth & development; Plant Proteins / genetics; Plant Proteins / metabolism; Plants, Genetically Modified / enzymology; Promoter Regions, Genetic; Signal Transduction; Tobacco / drug effects; Tobacco / enzymology; Tobacco / genetics; Tobacco / growth & development
TL;DR: The results suggest that the expression of CYP 82E4 is senescence-specific in the leaves and the use of the CYP82E4 promoter could provide a valuable tool for regulating gene expression in the senescing leaves. (via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger (Web of Science)
3. Good Health and Well-being (OpenAlex)
13. Climate Action (Web of Science)
Source: Web Of Science
Added: August 6, 2018

2008 journal article

Estimating the individual effects of the reduced palmitic acid fap(nc) and fap1 alleles on agronomic traits in two soybean populations

CROP SCIENCE, 48(2), 633–639.

By: A. Cardinal n, R. Dewey n & J. Burton*

TL;DR: After removing the effect of fap nc from the model, the genetic correlation between palmitate and yield was significant in one population but not significant between palMITate and height, indicating that fap1 has a small but significant effect on seed yield but no effect on plant height. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Source: Web Of Science
Added: August 6, 2018

2008 journal article

Mapping genes encoding microsomal omega-6 desaturase enzymes and their cosegregation with QTL affecting oleate content in soybean

CROP SCIENCE, 48(2), 640–650.

By: E. Bachlava n, R. Dewey n, J. Auclair n, S. Wang n, J. Burton* & A. Cardinal n

TL;DR: The genotyping assays developed for the FAD2-1A, FAD 2-1B, and Fad2-2B isoforms, as well as their linked simple sequence repeat markers, can be used in soybean breeding programs for the elevation of oleic acid seed content through marker-assisted selection. (via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger (OpenAlex)
Source: Web Of Science
Added: August 6, 2018

2008 journal article

Mutations in a Delta(9)-Stearoyl-ACP-Desaturase Gene Are Associated with Enhanced Stearic Acid Levels in Soybean Seeds

CROP SCIENCE, 48(6), 2305–2313.

TL;DR: A newly identifi ed soybean gene is reported, designated SACPD-C, that encodes a unique isoform of Δ 9 –stearoyl-ACP-desaturase, the enzyme responsible for converting stearic acid to oleic acid (18:1), suggesting that the encoded desaturase functions to enhance oleIC acid biosynthetic capacity as the immature seed is actively engaged in triacylglycerol production and storage. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Source: Web Of Science
Added: August 6, 2018

2008 journal article

RNA interference (RNAi)-induced suppression of nicotine demethylase activity reduces levels of a key carcinogen in cured tobacco leaves

PLANT BIOTECHNOLOGY JOURNAL, 6(4), 346–354.

By: R. Lewis n, A. Jack*, J. Morris*, V. Robert*, L. Gavilano*, B. Siminszky*, L. Bush*, A. Hayes*, R. Dewey n

author keywords: nicotine demethylase; N'-nitrosonornicotine; RNA interference; tobacco carcinogens; tobacco-specific nitrosamines (TSNAs)
MeSH headings : Carcinogens / analysis; Carcinogens / metabolism; Cytochrome P-450 Enzyme System / genetics; Cytochrome P-450 Enzyme System / metabolism; Gene Expression Regulation, Plant; Nitrosamines / analysis; Nitrosamines / metabolism; Plant Leaves / chemistry; Plant Leaves / metabolism; Plant Proteins / genetics; Plant Proteins / metabolism; RNA Interference; RNA, Plant / genetics; Tobacco / enzymology; Tobacco / genetics; Tobacco / metabolism
TL;DR: The inhibition of nicotine demethylase activity is an effective means of decreasing significantly the level of a key defined animal carcinogen present in tobacco products. (via Semantic Scholar)
Source: Web Of Science
Added: August 6, 2018

2007 journal article

Expression profiling on soybean leaves reveals integration of ER- and osmotic-stress pathways

BMC GENOMICS, 8.

By: A. Irsigler*, M. Costa*, P. Zhang n, P. Reis*, R. Dewey n, R. Boston n, E. Fontes*

MeSH headings : DNA, Complementary / genetics; Endoplasmic Reticulum / metabolism; Gene Expression Profiling / methods; Oligonucleotide Array Sequence Analysis; Osmosis; Plant Leaves / genetics; Plant Leaves / metabolism; Reverse Transcriptase Polymerase Chain Reaction; Signal Transduction / genetics; Signal Transduction / physiology; Soybeans / genetics; Soybeans / metabolism
TL;DR: This scenario indicates that polyethylene glycol (PEG)-induced cellular dehydration and ER stress elicited very different up-regulated responses within a 10-h stress treatment regime, and provides a list of candidate regulatory components, which may integrate the osmotic-stress and ER-stress signaling pathways in plants. (via Semantic Scholar)
Source: Web Of Science
Added: August 6, 2018

2007 journal article

Molecular analysis of soybean lines with low palmitic acid content in the seed oil

CROP SCIENCE, 47(1), 304–310.

By: A. Cardinal n, J. Burton*, A. Camacho-Roger n, J. Yang n, R. Wilson* & R. Dewey n

TL;DR: Three of the four unique FATB genes expressed in soybean are isolated and it is shown that the isoform designated GmFATB1a represents the specific gene deleted in lines possessing the fap nc locus. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Source: Web Of Science
Added: August 6, 2018

2006 journal article

A mutation in a 3-keto-acyl-ACP synthase II gene is associated with elevated palmitic acid levels in soybean seeds

CROP SCIENCE, 46(6), 2453–2459.

By: K. Aghoram n, R. Wilson*, J. Burton n & R. Dewey n

TL;DR: The objective of the present research was to define the molecular basis by which the fap 2 locus increases seed palmitate levels, and to isolate two closely related, yet unique KAS II cDNAs from soybean cultivar Century and its derivative highPalmitate germplasm C1727. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Source: Web Of Science
Added: August 6, 2018

2005 journal article

Conversion of nicotine to nornicotine in Nicotiana tabacum is mediated by CYP82E4, a cytochrome P450 monooxygenase

Proceedings of the National Academy of Sciences of the United States of America, 102(41), 14919–14924.

By: B. Siminszky, L. Gavilano, S. Bowen & R. Dewey

Source: NC State University Libraries
Added: August 6, 2018

2005 journal article

Conversion of nicotine to nornicotine in Nicotiana tabacum is mediated by CYP82E4, a cytochrome P450 monooxygenase

Proceedings of the National Academy of Sciences, 102(41), 14919–14924.

By: B. Siminszky n, L. Gavilano n, S. Bowen n & R. Dewey n

author keywords: N '-nitrosonornicotine; N-demethylation; tobacco; alkaloid; tobacco-specific nitrosamines
MeSH headings : Base Sequence; Cloning, Molecular; Cytochrome P-450 Enzyme System / metabolism; DNA Primers; Expressed Sequence Tags; Gene Library; Microarray Analysis; Mixed Function Oxygenases / genetics; Mixed Function Oxygenases / metabolism; Molecular Sequence Data; Nicotine / analogs & derivatives; Nicotine / biosynthesis; Nicotine / chemistry; Nicotine / metabolism; Plants, Genetically Modified; RNA Interference; Sequence Analysis, DNA; Tobacco / metabolism; Yeasts
TL;DR: This work used a microarray-based strategy to identify genes that are differentially regulated between closely related tobacco lines that accumulate either nicotine (nonconverters) or nornicotine (conversters) as the predominant alkaloid in the cured leaf. (via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being (Web of Science; OpenAlex)
Source: Crossref
Added: August 28, 2020

2005 journal article

Free amino acid profiles suggest a possible role for asparagine in the control of storage-product accumulation in developing seeds of low- and high-protein soybean lines

JOURNAL OF EXPERIMENTAL BOTANY, 56(417), 1951–1963.

By: C. Hernandez-Sebastia*, F. Marsolais*, C. Saravitz n, D. Israel n, R. Dewey n & S. Huber*

author keywords: amino acid profile; carbon partitioning; seed nitrogen supply; seed protein content; soybean
MeSH headings : Amino Acids / metabolism; Asparagine / physiology; Cotyledon / physiology; Gene Expression Regulation, Developmental; Gene Expression Regulation, Plant; Light; Plant Oils / metabolism; Plant Proteins / biosynthesis; Seeds / physiology; Seeds / radiation effects; Soybeans / embryology; Soybeans / metabolism; Soybeans / radiation effects; Transcription, Genetic
TL;DR: The hypothesis that high seed-protein content in soybean is determined by the capacity of the embryo to take up nitrogen sources and to synthesize storage proteins is supported. (via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger (Web of Science)
Source: Web Of Science
Added: August 6, 2018

2005 journal article

Oleate desaturase enzymes of soybean: evidence of regulation through differential stability and phosphorylation

PLANT JOURNAL, 44(3), 433–446.

By: G. Tang n, W. Novitzky n, H. Griffin n, S. Huber* & R. Dewey n

author keywords: oleate desaturase; FAD2; protein phosphorylation; 26S proteasome; polyunsaturated fatty acids; soybean (Glycine max)
MeSH headings : Amino Acid Sequence; Enzyme Stability; Fatty Acid Desaturases / chemistry; Fatty Acid Desaturases / genetics; Fatty Acid Desaturases / metabolism; Gene Expression Regulation, Enzymologic; Gene Expression Regulation, Plant; Hot Temperature; Isoenzymes / genetics; Isoenzymes / metabolism; Molecular Sequence Data; Oxidoreductases Acting on CH-CH Group Donors / chemistry; Oxidoreductases Acting on CH-CH Group Donors / genetics; Oxidoreductases Acting on CH-CH Group Donors / metabolism; Phosphorylation; Saccharomyces cerevisiae / genetics; Sequence Homology, Amino Acid; Soybeans / enzymology; Soybeans / genetics
TL;DR: Results show that post-translational regulatory mechanisms are likely to play an important role in modulating FAD2-1 enzyme activities. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Source: Web Of Science
Added: August 6, 2018

2003 journal article

A novel C-terminal proteolytic processing of cytosolic pyruvate kinase, its phosphorylation and degradation by the proteasome in developing soybean seeds

PLANT JOURNAL, 34(1), 77–93.

By: G. Tang n, S. Hardin n, R. Dewey n & S. Huber n

author keywords: pyruvate kinase; phosphorylation site; C-terminal truncation; degradation; proteasome; soybean (Glycine max (L) cv Ransom)
MeSH headings : Amino Acid Sequence; Cytosol / enzymology; Molecular Weight; Peptide Hydrolases / metabolism; Phosphorylation; Phylogeny; Proteasome Endopeptidase Complex; Protein Processing, Post-Translational; Protein Subunits / chemistry; Protein Subunits / metabolism; Pyruvate Kinase / chemistry; Pyruvate Kinase / metabolism; Seeds / enzymology; Seeds / metabolism; Soybeans / enzymology; Soybeans / metabolism
TL;DR: The results suggest that there are two pathways for PyrKinc modification at the post-translational level: one involves partial C-terminal truncation to generate a 51 kDa pyruvate kinase subunit which might have altered regulatory properties and the other involves phosphorylation and ubiquitin conjugation that targets the protein to the 26S proteasome for complete degradation. (via Semantic Scholar)
Source: Web Of Science
Added: August 6, 2018

2003 journal article

Co-expression of a NADPH : P450 reductase enhances CYP71A10-dependent phenylurea metabolism in tobacco

PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY, 77(2), 35–43.

By: B. Siminszky*, A. Freytag*, B. Sheldon n & R. Dewey n

UN Sustainable Development Goal Categories
2. Zero Hunger (Web of Science)
3. Good Health and Well-being (OpenAlex)
13. Climate Action (Web of Science)
Source: Web Of Science
Added: August 6, 2018

2001 journal article

Effect of alleles governing 16 : 0 concentration on glycerolipid composition in developing soybeans

JOURNAL OF THE AMERICAN OIL CHEMISTS SOCIETY, 78(4), 329–334.

author keywords: developing-seed; fap-alleles; genetics; glycerolipid composition; Glycine max; logistic function; metabolism; oil; palmitic acid; saturated fat
TL;DR: The fap alleles apparently do not encode or regulate the activities of glycerolipid acyltransferase enzymes, and major genetic effects on triacylglycerol composition accrue through regulation of palmitic acid production in the plastids of developing soybean cotyledons. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Source: Web Of Science
Added: August 6, 2018

2001 journal article

Hyperosmotic stress induces the rapid phosphorylation of a soybean phosphatidylinositol transfer protein homolog through activation of the protein kinases SPK1 and SPK2

PLANT CELL, 13(5), 1205–1219.

By: D. Monks n, K. Aghoram n, P. Courtney n, D. DeWald* & R. Dewey n

MeSH headings : Carrier Proteins / metabolism; Cell Cycle Proteins; Checkpoint Kinase 2; Enzyme Activation; Membrane Proteins; Models, Biological; Osmotic Pressure; Phosphatidylinositol Phosphates / metabolism; Phosphatidylinositols / metabolism; Phospholipid Transfer Proteins; Phosphorylation; Plant Proteins / metabolism; Plants, Genetically Modified; Plants, Toxic; Protein Serine-Threonine Kinases / metabolism; Saccharomyces cerevisiae Proteins; Signal Transduction; Soybeans; Tobacco
TL;DR: The activation of SPK1 and/or SPK2 and the subsequent phosphorylation of Ssh1p are revealed as two early successive events in a hyperosmotic stress–induced signaling cascade in plants, which suggests that the ultimate function of SSh1p in cellular signaling is to alter the plant’s capacity to synthesize phosphoinositides during periods of hyperosMotic stress. (via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger (Web of Science)
13. Climate Action (Web of Science)
Source: Web Of Science
Added: August 6, 2018

2001 journal article

Induction of lipid metabolic enzymes during the endoplasmic reticulum stress response in plants

PLANT PHYSIOLOGY, 126(1), 267–277.

By: K. Shank n, P. Su n, I. Brglez n, W. Boss n, R. Dewey* & R. Boston n

MeSH headings : 1-Phosphatidylinositol 4-Kinase / metabolism; Choline-Phosphate Cytidylyltransferase / metabolism; Diacylglycerol Kinase / metabolism; Endoplasmic Reticulum / metabolism; Lipid Metabolism; Phosphotransferases (Alcohol Group Acceptor) / metabolism; Seeds / growth & development; Soybeans / embryology; Soybeans / enzymology; Soybeans / genetics; Soybeans / metabolism; Tunicamycin / pharmacology
TL;DR: It is postulate that the plant ER stress response has an important role in general plant metabolism, and more specifically in integrating the synthesis of protein and lipid reserves to allow proper seed formation. (via Semantic Scholar)
Source: Web Of Science
Added: August 6, 2018

2001 journal article

Metabolic mechanisms associated with alleles governing the 16 : 0 concentration of soybean oil

JOURNAL OF THE AMERICAN OIL CHEMISTS SOCIETY, 78(4), 335–340.

By: R. Wilson n, T. Marquardt n, W. Novitzky n, J. Burton n, . Wilcox*, A. Kinney*, R. Dewey n

author keywords: developing-seed; fap-alleles; genetics; glycerolipid composition; Glycine max; metabolism; oil; palmitic acid; phospholipid; saturated fat
TL;DR: These metabolic studies narrowed the identification of fap1, fap2, and fapnc alleles to the genes that encode or regulate the 3-keto-acyl-ACP synthetase II (where ACP is acyl carrier protein), 16∶0-ACP thioesterase, 18∷0- ACP desaturase, or 18∶1-ACPThioester enzyme enzymes. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Source: Web Of Science
Added: August 6, 2018

2000 journal article

A cytochrome P450 monooxygenase cDNA (CYP71A10) confers resistance to linuron in transgenic Nicotiana tabacum

WEED SCIENCE, 48(3), 291–295.

By: B. Siminszky n, B. Sheldon n, F. Corbin n & R. Dewey n

author keywords: cytochrome P450; linuron; Glycine max L. Merr 'Dare', soybean; Nicotiana tabacum L. 'SR1', tobacco; herbicide metabolism; phenylurea; genetic engineering; metabolic engineering
TL;DR: Evidence is provided that plant-derived P450 genes can be employed effectively to confer herbicide resistance to transgenic plants and this line was more tolerant to linuron than the wild type for both application methods. (via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger (Web of Science)
13. Climate Action (Web of Science)
Source: Web Of Science
Added: August 6, 2018

2000 journal article

Antisense and sense expression of a sucrose binding protein homologue gene from soybean in transgenic tobacco affects plant growth and carbohydrate partitioning in leaves

PLANT SCIENCE, 152(1), 87–98.

By: J. Pedra, N. Delu, C. Pirovani, L. Contim, R. Dewey*, W. Otoni, E. Fontes

author keywords: Nicotiana; soybean; sucrose binding protein; sucrose transport
TL;DR: Results indicate that S-64 protein is functionally analogous to SBP, representing an important component of the sucrose translocation pathway in plants, and both antisense repression and overexpression of the SBP homologue in transgenic lines altered carbohydrate partitions in mature leaves. (via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being (Web of Science; OpenAlex)
Source: Web Of Science
Added: August 6, 2018

2000 patent

Cytochrome P-450 constructs and method of producing herbicide-resistant transgenic plants

Washington, DC: U.S. Patent and Trademark Office.

By: B. Siminszky, R. Dewey & F. Corbin

Source: NC State University Libraries
Added: August 6, 2018

1999 journal article

Characterization of aminoalcoholphosphotransferases from Arabidopsis thaliana and soybean

PLANT PHYSIOLOGY AND BIOCHEMISTRY, 37(6), 445–457.

By: J. Goode n & R. Dewey n

author keywords: cholinephosphotransferase ethanolaminephosphotransferase; Ca2+ inhibition; CMP inhibition; phospholipid biosynthesis
TL;DR: In overexpression of the soybean AAPT cDNA in transgenic tobacco using a strong constitutive promoter resulted in only modest increases in enzymatic activity, suggesting the possibility of post-transcriptional regulation. (via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being (Web of Science; OpenAlex)
Source: Web Of Science
Added: August 6, 2018

1999 journal article

Expression of a soybean cytochrome P450 monooxygenase cDNA in yeast and tobacco enhances the metabolism of phenylurea herbicides

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 96(4), 1750–1755.

By: B. Siminszky n, F. Corbin n, E. Ward n, T. Fleischmann n & R. Dewey n

MeSH headings : Cloning, Molecular; Cytochrome P-450 Enzyme System / genetics; Cytochrome P-450 Enzyme System / metabolism; Herbicides / pharmacokinetics; Inactivation, Metabolic; Insecticides / pharmacokinetics; Kinetics; Microsomes / enzymology; Mixed Function Oxygenases / genetics; Mixed Function Oxygenases / metabolism; Molecular Sequence Data; Phenylurea Compounds / pharmacokinetics; Plants, Genetically Modified; Plants, Toxic; Saccharomyces cerevisiae / enzymology; Saccharomyces cerevisiae / genetics; Soybean Proteins; Soybeans / enzymology; Soybeans / genetics; Substrate Specificity; Tobacco / enzymology; Tobacco / genetics
TL;DR: In vitro enzyme assays showed that the gene product of one heterologously expressed P450 cDNA (CYP71A10) specifically catalyzed the metabolism of phenylurea herbicides, converting four herbicides of this class into more polar compounds. (via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger (Web of Science)
3. Good Health and Well-being (OpenAlex)
13. Climate Action (Web of Science)
Source: Web Of Science
Added: August 6, 2018

1999 review

Phosphoinositide kinases and the synthesis of polyphosphoinositides in higher plant cells

[Review of ]. INTERNATIONAL REVIEW OF CYTOLOGY - A SURVEY OF CELL BIOLOGY, VOL 189, 189, 95–130.

By: B. Drobak*, R. Dewey n & W. Boss n

author keywords: phosphatidylinositol; kinases; inositol; plants; biosynthesis; cell signaling; phosphatidylinositol transfer proteins; phospholipids
MeSH headings : Animals; Eukaryotic Cells / metabolism; Humans; Phosphatidylinositol 3-Kinases / metabolism; Phosphatidylinositol Phosphates / biosynthesis; Phosphatidylinositols / metabolism; Phosphotransferases / metabolism; Plant Cells; Plants / metabolism
TL;DR: The author focuses on the phosphoinositide kinases responsible for the synthesis of all phosphorylated forms of phosphatidylinositol, which, in his opinion, are most likely to provide a suitable starting point for further research into the role of phosphorusoinositides in plants. (via Semantic Scholar)
Source: Web Of Science
Added: August 6, 2018

1998 patent

Modified urf13-T protein

Washington, DC: U.S. Patent and Trademark Office.

Ralph Dewey; Charles S. Levings, III

Source: NC State University Libraries
Added: August 6, 2018

1998 journal article

Novel developmentally regulated phosphoinositide binding proteins from soybean whose expression bypasses the requirement for an essential phosphatidylinositol transfer protein in yeast

EMBO JOURNAL, 17(14), 4004–4017.

By: M. Kearns*, D. Monks n, M. Fang*, M. Rivas*, P. Courtney n, J. Chen*, G. Prestwich*, A. Theibert*, R. Dewey n, V. Bankaitis*

author keywords: phosphatidylinositol transfer proteins; phosphoinositides; signaling; soybean; stress response
MeSH headings : Amino Acid Sequence; Carrier Proteins / genetics; Carrier Proteins / metabolism; Cell Membrane / metabolism; Cloning, Molecular; Cytosol / metabolism; Gene Expression Regulation, Plant / physiology; Genes, Plant / genetics; Membrane Proteins; Molecular Sequence Data; Osmolar Concentration; Phosphatidylinositols / metabolism; Phospholipid Transfer Proteins; Phosphorylation; Plant Proteins / metabolism; Protein Binding; RNA, Messenger / analysis; RNA, Plant / analysis; Recombinant Fusion Proteins; Saccharomyces cerevisiae / genetics; Saccharomyces cerevisiae / metabolism; Saccharomyces cerevisiae Proteins; Sequence Homology, Amino Acid; Sodium Chloride; Sorbitol; Soybeans / genetics; Soybeans / metabolism
TL;DR: This work reports the identification and characterization of two soybean proteins, Ssh1p and Ssh2p, whose structural genes were recovered on the basis of their abilities to rescue the viability of PITP‐deficient Saccharomyces cerevisiae strains and shows that Ssh 1p is phosphorylated in response to various environmental stress conditions, including hyperosmotic stress. (via Semantic Scholar)
Source: Web Of Science
Added: August 6, 2018

1997 patent

Maize cytoplasmic male sterility type T (cms-T) mitochondria DNA

Washington, DC: U.S. Patent and Trademark Office.

Ralph Dewey; Charles S. Levings, III

Source: NC State University Libraries
Added: August 6, 2018

1997 patent

Method of detecting toxins using host cells expressing an urf13-T gene

Washington, DC: U.S. Patent and Trademark Office.

By: C. Levings, R. Dewey & C. Braun

Source: NC State University Libraries
Added: August 6, 2018

1997 chapter

Phosphatidylcholine biosynthesis in soybeans: The cloning and characterization of genes encoding enzymes of the nucleotide pathway

In M. U. K. J. P. Williams & N. W. Lem (Eds.), Physiology, biochemistry, and molecular biology of plant lipids (pp. 110–112).

By: D. Monks n, J. Goode n, P. Dinsmore n & R. Dewey n

Ed(s): M. J. P. Williams & N. Lem

TL;DR: The isolation and characterization of the genes encoding enzymes of this consolidated pathway will accelerate the study of PC biosynthesis in higher plants and help elucidate the processes by which plants regulate the production of this important phospholipid at both the molecular and cellular levels. (via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger (OpenAlex)
Source: NC State University Libraries
Added: August 6, 2018

1996 patent

Method of detecting toxin-insensitive urf13-T protein

Washington, DC: U.S. Patent and Trademark Office.

By: C. Levings, R. Dewey & C. Braun

Source: NC State University Libraries
Added: August 6, 2018

1995 patent

Modified unF-13 protein and gene

Washington, DC: U.S. Patent and Trademark Office.

By: C. Levings, R. Dewey & C. Braun

Source: NC State University Libraries
Added: August 6, 2018

1994 journal article

THE AAPT1 GENE OF SOYBEAN COMPLEMENTS A CHOLINEPHOSPHOTRANSFERASE-DEFICIENT MUTANT OF YEAST

PLANT CELL, 6(10), 1495–1507.

By: R. Dewey n, R. Wilson n, W. Novitzky n & J. Goode n

MeSH headings : Amino Acid Sequence; Base Sequence; Cloning, Molecular; Cytidine Diphosphate / analogs & derivatives; Cytidine Diphosphate / pharmacology; DNA, Complementary / genetics; Diacylglycerol Cholinephosphotransferase / drug effects; Diacylglycerol Cholinephosphotransferase / genetics; Diacylglycerol Cholinephosphotransferase / metabolism; Escherichia coli / genetics; Ethanolaminephosphotransferase / deficiency; Ethanolaminephosphotransferase / genetics; Ethanolamines / pharmacology; Gene Expression Regulation, Plant; Genes, Plant / genetics; Genetic Complementation Test; Molecular Sequence Data; Plant Proteins / drug effects; Plant Proteins / genetics; Plant Proteins / metabolism; Saccharomyces cerevisiae / enzymology; Saccharomyces cerevisiae / genetics; Seeds / enzymology; Seeds / genetics; Seeds / growth & development; Sequence Homology, Amino Acid; Soybean Proteins; Soybeans / enzymology; Soybeans / genetics; Transcription, Genetic
TL;DR: Assays of soybean AAPT1-encoded enzyme activity in yeast microsomal membranes revealed that the addition of CDP-ethanolamine to the reaction inhibited incorporation of 14C-CDP-choline into phosphatidylcholine in a manner very similar to that observed using unlabeled CDP -choline. (via Semantic Scholar)
Source: Web Of Science
Added: August 6, 2018

1991 journal article

CHIMERIC MITOCHONDRIAL GENES EXPRESSED IN THE C-MALE-STERILE CYTOPLASM OF MAIZE

CURRENT GENETICS, 20(6), 475–482.

By: R. Dewey n, D. Timothy n & C. Levings n

author keywords: CYTOPLASMIC MALE STERILITY; ATP9; ATP6; COXII; RECOMBINATION; MITOCHONDRIAL DNA
MeSH headings : Adenosine Triphosphatases / genetics; Amino Acid Sequence; Base Sequence; Blotting, Northern; Cytoplasm / metabolism; DNA; Electron Transport Complex IV / genetics; Mitochondria / metabolism; Molecular Sequence Data; Recombination, Genetic; Reproduction / genetics; Zea mays / genetics; Zea mays / ultrastructure
TL;DR: Aberrant recombinations involving the mitochondrial atp9, atp6 and coxII genes have created unique chimeric sequences in the C male0sterile cytoplasm (cms-C) of maize, and their novel arrangements and the strong correlation between these genes and the C type of male sterility suggest such a role. (via Semantic Scholar)
UN Sustainable Development Goal Categories
13. Climate Action (Web of Science)
Source: Web Of Science
Added: August 6, 2018

1986 journal article

Novel recombinations in the maize mitochondrial genome produce a unique transcriptional unit in the texas male-sterile cytoplasm

Cell, 44(3), 439–449.

By: R. Dewey n, C. Levings n & D. Timothy n

MeSH headings : Adenosine Triphosphatases / genetics; Base Sequence; DNA, Mitochondrial / genetics; Genes, Regulator; Nucleic Acid Hybridization; RNA / genetics; RNA, Mitochondrial; RNA, Transfer, Amino Acyl / genetics; Recombination, Genetic; Repetitive Sequences, Nucleic Acid; Ribosomes; Sequence Homology, Nucleic Acid; Transcription, Genetic; Zea mays / genetics; Zea mays / physiology; Zea mays / ultrastructure
TL;DR: A 3547 bp DNA fragment from male-sterile maize mitochondria, designated TURF 2H3, selected because of its unique and abundant transcripts appears to be uniquely altered in cms-T plants restored to fertility by the nuclear restorer genes Rf1 and Rf2. (via Semantic Scholar)
UN Sustainable Development Goal Categories
13. Climate Action (Web of Science)
Source: NC State University Libraries
Added: August 6, 2018

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