@article{gaire_devries_mick_santangelo_bottillo_camera_schal_2021, title={Human skin triglycerides prevent bed bug (Cimex lectularius L.) arrestment}, volume={11}, ISSN={["2045-2322"]}, DOI={10.1038/s41598-021-01981-1}, abstractNote={AbstractBed bugs (Cimex lectularius) have proliferated globally and have become one of the most challenging pests to control indoors. They are nocturnal and use multiple sensory cues to detect and orient towards their human hosts. After feeding, usually on a sleeping human, they return to a shelter on or around the sleeping surface, but not directly on the host. We hypothesized that although human skin odors attract hungry bed bugs, human skin compounds may also prevent arrestment on hosts. We used arrestment assays to test human skin swabs, extracts from human skin swabs, and pure compounds identified from human skin swabs. When given a choice, bed bugs preferred to arrest on substrates not previously conditioned by humans. These responses were consistent among laboratory-reared and apartment-collected bed bugs. The compounds responsible for this behavior were found to be extractable in hexane, and bed bugs responded to such extracts in a dose-dependent manner. Bioassay-guided fractionation paired with thin-layer chromatography, GC–MS, and LC–MS analyses suggested that triglycerides (TAGs), common compounds found on human skin, were preventing arrestment on shelters. Bed bugs universally avoided sheltering in TAG-treated shelters, which was independent of the number of carbons or the number of double bonds in the TAG. These results provide strong evidence that the complex of human skin compounds serve as multifunctional semiochemicals for bed bugs, with some odorants attracting host-seeking stages, and others (TAGs and possibly other compounds) preventing bed bug arrestment. Host chemistry, environmental conditions and the physiological state of bed bugs likely influence the dual nature behavioral responses of bed bugs to human skin compounds.}, number={1}, journal={SCIENTIFIC REPORTS}, author={Gaire, Sudip and DeVries, Zachary C. and Mick, Russell and Santangelo, Richard G. and Bottillo, Grazia and Camera, Emanuela and Schal, Coby}, year={2021}, month={Dec} }
 @article{gaire_schal_mick_devries_2020, title={The Role of Antennae in Heat Detection and Feeding Behavior in the Bed Bug (Hemiptera: Cimicidae)}, volume={113}, ISSN={["1938-291X"]}, url={https://doi.org/10.1093/jee/toaa250}, DOI={10.1093/jee/toaa250}, abstractNote={AbstractThe common bed bug (Cimex lectularius L.) is an obligate hematophagous ectoparasite that has significant impacts on human health and well-being. All life stages of bed bugs (except eggs) feed solely on blood, which is required to molt and reproduce. Bed bugs use multiple cues to locate their hosts, including heat, CO2, and body odors. Of these cues, detection of heat appears limited to a short distance of <3 cm. However, it remains unclear if bed bugs can detect radiant heat, what structure(s) are responsible for heat detection, and if heat detection via the antennae is required for feeding. In this study, bed bug response to radiant heat was evaluated using the two-choice T-maze assay with the heat source either in contact with the surface (i.e., conduction) or not in contact (i.e., radiation) in nonantennectomized bed bugs. Further, we systematically ablated the bed bug’s antennal segments (distal tip, first segment, and all four segments) and assessed their responses to heat and feeding in a unique two-choice T-maze assay and individual feeding assays, respectively. Our two-choice assays with contact to or no contact with the surface indicated that bed bugs cannot detect radiant heat. Later, we found that the distal tip of the terminal antennal segment is responsible for orientation toward a heat source. However, >50% of the bed bugs fed even when the entire antenna was removed, suggesting redundancy in sensory cues that drive feeding. These results will be used to better understand the role heat plays in bed bug host attraction and design of traps.}, number={6}, journal={JOURNAL OF ECONOMIC ENTOMOLOGY}, publisher={Oxford University Press (OUP)}, author={Gaire, Sudip and Schal, Coby and Mick, Russell and DeVries, Zachary}, editor={Appel, ArthurEditor}, year={2020}, month={Dec}, pages={2858–2863} }
 @article{devries_santangelo_crissman_mick_schal_2019, title={Exposure risks and ineffectiveness of total release foggers (TRFs) used for cockroach control in residential settings}, volume={19}, ISSN={1471-2458}, url={http://dx.doi.org/10.1186/s12889-018-6371-z}, DOI={10.1186/s12889-018-6371-z}, abstractNote={The German cockroach, Blattella germanica, is one of the most challenging pests to eradicate from indoor environments. Professional pest control is often prohibitively expensive, prompting low-income residents to turn to over-the-counter consumer products, including total release foggers (TRFs, "bug bombs"). Despite their widespread use, little is known regarding either the associated pesticide exposure risks or the efficacy of TRFs. Cockroach-infested homes were recruited into the study. Wipe samples were collected from various surfaces before TRFs were discharged, immediately after, and one month later to determine pesticide exposure risks in 20 homes (divided equally among four different TRF products). Simultaneously, cockroach populations were monitored in all homes to assess the efficacy of TRFs. In parallel, 10 homes were treated with gel baits (divided equally between two bait products), to compare TRFs to a more targeted, low-risk, do-it-yourself intervention strategy. TRFs failed to reduce cockroach populations, whereas similarly priced gel baits caused significant declines in the cockroach populations. Use of TRFs resulted in significant pesticide deposits throughout the kitchen. Across all products, pesticides, and horizontal kitchen surfaces, pesticide residues following TRF discharge were 603-times (SEM ±184) higher than baseline, with a median increase of 85 times. The high risks of pesticide exposure associated with TRFs combined with their ineffectiveness in controlling German cockroach infestations call into question their utility in the marketplace, especially because similarly priced and much safer bait products are highly effective in the indoor environment.}, number={1}, journal={BMC Public Health}, publisher={Springer Science and Business Media LLC}, author={DeVries, Zachary C. and Santangelo, Richard G. and Crissman, Jonathan and Mick, Russell and Schal, Coby}, year={2019}, month={Jan} }
 @article{devries_saveer_mick_schal_2018, title={Bed Bug (Hemiptera: Cimicidae) Attraction to Human Odors: Validation of a Two-Choice Olfactometer}, volume={56}, ISSN={0022-2585 1938-2928}, url={http://dx.doi.org/10.1093/jme/tjy202}, DOI={10.1093/jme/tjy202}, abstractNote={AbstractBed bugs (Cimex lectularius L.) (Hemiptera: Cimicidae) are obligate hematophagous ectoparasites, and, therefore, must locate suitable hosts to ensure survival and reproduction. Their largely nocturnal activity suggests that chemosensory and thermosensory cues would play critical roles in host location. Yet, the importance of olfaction in host attraction of bed bugs remains unclear. We developed and validated a Y-tube, two-choice olfactometer and tested its suitability for investigating attraction to human odors (from skin swabs). Olfactometer orientation significantly affected the percentage of bed bugs that were activated by human odors, with significantly more bed bugs responding when the olfactometer was oriented vertically (bug introduced at bottom of the olfactometer) compared with all other orientations. Starved (7–10 d) adult males, mated females, and nymphs responded (47–77% moved up the olfactometer and made a choice) when human odors were present in the olfactometer, while starved, unmated females did not respond. Skin swabs from all five human participants elicited high response rates (65–82%), and bed bugs from four different populations responded to skin swabs (40–82% response rate). However, in all assays including those resulting in relatively low response rates, bed bugs exhibited >90% preference for human odors over blank controls. These results provide strong evidence that bed bugs can respond and orient towards human odors, independently of all other host cues. Furthermore, the validated olfactometer should enable rapid and efficient evaluations of bed bug behavioral responses to semiochemicals.}, number={2}, journal={Journal of Medical Entomology}, publisher={Oxford University Press (OUP)}, author={DeVries, Zachary C and Saveer, Ahmed M and Mick, Russell and Schal, Coby}, year={2018}, month={Nov}, pages={362–367} }
 @article{devries_mick_balvín_schal_2017, title={Aggregation behavior and reproductive compatibility in the family Cimicidae}, volume={7}, ISSN={2045-2322}, url={http://dx.doi.org/10.1038/s41598-017-12735-3}, DOI={10.1038/s41598-017-12735-3}, abstractNote={AbstractBed bugs (Cimex lectularius) provide a unique opportunity to understand speciation and host-associated divergence in parasites. Recently, two sympatric but genetically distinct lineages ofC. lectulariuswere identified: one associated with humans and one associated with bats. We investigated two mechanisms that could maintain genetic differentiation in the field: reproductive compatibility (via mating crosses) and aggregation fidelity (via two-choice sheltering assays). Effects were assessed at the intra-lineage level (within human-associated bed bugs), inter-lineage level (between human- and bat-associated bed bugs), and inter-species level (betweenC. lectulariusandCimex pipistrelli[bat bug]). Contrary to previous reports, bed bugs were found to be reproductively compatible at both the intra- and inter-lineage levels, but not at the inter-species level (although three hybrids were produced, one of which developed into an adult). Lineage- and species-specific aggregation fidelity was only detected in 8% (4 out of 48) of the aggregation fidelity assays run. These results indicate that under laboratory conditions, host-associated lineages of bed bugs are reproductively compatible, and aggregation pheromones are not capable of preventing gene flow between lineages.}, number={1}, journal={Scientific Reports}, publisher={Springer Science and Business Media LLC}, author={DeVries, Zachary and Mick, Russell and Balvín, Ondřej and Schal, Coby}, year={2017}, month={Oct} }