@article{andersen_clark_hopper_hodson_schilling_daniels_woods_kovach_berlinsky_kenter_et al._2021, title={Methods of domestic striped bass (Morone saxatilis) spawning that do not require the use of any hormone induction}, volume={533}, ISSN={["1873-5622"]}, DOI={10.1016/j.aquaculture.2020.736025}, abstractNote={Nineteen batch spawning trials were conducted using 5th and 6th generation domestic striped bass (Morone saxatilis) to demonstrate the ability of these fish to volitionally spawn in large tanks to produce larvae using only photothermal and salinity conditioning. The findings described are the first report of multiple striped bass successfully batch spawning in captivity without exogenous hormone administration. The results of these trials indicate that an approximately 1:1 ratio of female to male striped bass in a single batch spawning unit is more favorable for production, that a minimum of at least 10 fish of each sex is required to elicit this particular spawning behavior, and that using 25 fish of each sex will yield commercially scalable larval production. This batch spawning method has been employed to effectively and consistently spawn over half of the female striped bass in the National Program for Genetic Improvement and Selective Breeding for the Hybrid Striped Bass Industry (N = 202 of 334 female fish over five years) to produce 44,608,181 swim-up larvae (26.6% hatching rate). Microsatellite genotyping and parentage assignment demonstrates that females will reproduce with between 2 and 18 males and that males will reproduce with between 1 and 6 females. Moreover, the effective broodstock size (Nb) of these batch spawning units is 33 and when accounting for multiple partners and unequal family sizes (Nbv) is 28. Lastly, the reported results include the successful spawning of female striped bass staged at and beyond 15 Bayless hours, or those that would have previously been considered ineligible for spawning even with the use of exogenous hormone treatment.}, journal={AQUACULTURE}, author={Andersen, L. K. and Clark, R. W. and Hopper, M. S. and Hodson, R. G. and Schilling, J. and Daniels, H. V. and Woods, L. C., III and Kovach, A. I. and Berlinsky, D. L. and Kenter, L. W. and et al.}, year={2021}, month={Feb} }
 @article{andersen_abernathy_berlinsky_bolton_booker_borski_brown_cerino_ciaramella_clark_et al._2021, title={The status of striped bass, <scp><i>Morone saxatilis</i>,</scp> as a commercially ready species for U.S. marine aquaculture}, volume={52}, ISSN={0893-8849 1749-7345}, url={http://dx.doi.org/10.1111/jwas.12812}, DOI={10.1111/jwas.12812}, abstractNote={Striped bass, Morone saxatilis , is an anadromous fish native to the North American Atlantic Coast and is well recognized as one of the most important and highly regarded recreational fisheries in the United States. Decades of research have been conducted on striped bass and its hybrid (striped bass (cid:1) white bass Morone chrysops ) and culture methods have been established, particularly for the hybrid striped bass, the fourth largest finfish aquaculture industry in the United States (US $50 million). Domesticated striped bass have been developed since the 1990s and broodstock are available from the government for commercial fry production using novel hormone-free methods along with traditional hormone-induced tank and strip spawning. No commercial-scale intensive larval rearing technologies have been developed at present and current fingerling production is conducted in fertilized freshwater ponds. Larval valued at about US $8.45 to US $9.25 per kg whole; the farm gate value for cultured striped bass may be as much as US $10.00 or more per kg depending on demand and market. The ideal market size is between 1.36 and 2.72 kg/fish, which is considerably larger than the traditional 0.68 to 0.90 kg/fish for the hybrid striped bass market. 0.57 0.91 US or US for 2.5 lb or fish). Recent for striped bass in in ranged from US $26.45 to US $41.89 per kg (US $12.00 – US $19.00 per lb) for boneless, skin-on fillets of wild caught striped bass. Market surveys conducted with Locals Seafood in North Carolina found that marketing value-added, boneless, skin-on fillets of aquacultured striped bass in the mid-Atlantic region is feasible even with a final product price of US $39.68 per kg (US $18.00 per lb). Based on these survey data, we estimate the U.S. farm gate value for striped bass can be as low as US $10.14 per kg (US $4.60 per lb) and as high as US $13.23 per kg (US $6.00 per lb) based on a 50.0% to 70.0% mark-up margin. Furthermore, assessments have shown consumer willingness to pay premium prices for striped bass (Quagrainie, 2019). These data show a clear economic and market potential for aquaculture production of striped bass, which already has a wide consumer acceptance and appeal.}, number={3}, journal={Journal of the World Aquaculture Society}, publisher={Wiley}, author={Andersen, Linnea K. and Abernathy, Jason and Berlinsky, David L. and Bolton, Greg and Booker, Matthew M. and Borski, Russell J. and Brown, Travis and Cerino, David and Ciaramella, Michael and Clark, Robert W. and et al.}, year={2021}, month={May}, pages={710–730} }
 @article{andersen_clark_mcginty_hopper_kenter_salger_schilling_hodson_kovach_berlinsky_et al._2021, title={Volitional tank spawning of domestic striped bass (Morone saxatilis) using human chorionic gonadotropin (hCG) and gonadotropin releasing hormone analogue (GnRHa)- induced 'pace-setting' females}, volume={532}, ISSN={["1873-5622"]}, DOI={10.1016/j.aquaculture.2020.735967}, abstractNote={Seventy-one tank spawning trials were conducted to evaluate the efficacy of exogenous hormone compounds and a novel “pace-set” strategy for inducing volitional tank spawning behavior in 5th generation domestic striped bass. Female fish (4.74 ± 0.73 kg; mean ± standard deviation) were treated with human chorionic gonadotropin (hCG; 29 trials), gonadotropin releasing hormone analog (GnRHa; 39 trials) or received no hormone treatment (control; 3 trials). Spawning trials were conducted using single females placed in spawning tanks with two (12 trials) or three (38 trials) males or with paired females placed in spawning tanks with three (4 trials) males. Significant differences in egg production, fry production, hatching rate, and fry/kg female body weight were generally not observed between exogenous hormone treatment groups (alpha = 0.05), with the exception of egg production differing between paired females spawning with three males (Student's t-test, p = 0.0255). However, a trend suggesting that increasing the number of males or females within the tanks improves yield of larvae (fry/kg female body weight) was observed. The untreated control females failed to spawn within 7 days. The pace-set spawning was conducted whereby one female treated with either hCG (7 trials) or GnRHa (7 trials) was placed in a spawning tank with one untreated female and multiple males. The results of these trials show for the first time that a hormone-induced female striped bass can be used to stimulate successful volitional spawning of an untreated female in the same tank with fry/kg female body weight production similar to that of hormone-treated fish. Microsatellite-based parentage of select tank spawns and four additional trials conducted with an increased number of males (19 trials total) showed that female striped bass typically spawn with at least two males; a single pair mating was only observed for one spawning trial. These data allowed for the determination of effective broodstock size (Nb) of each tank spawning trial at between 2.00 and 5.60 when considering all male contributions. The Nb generally increased as the number of males and female fish in the tank increased (from 2.53 for one female and two males to 5.52 for two females and six males). These results indicate that domestic striped bass are promiscuous and will generally reproduce in captivity using tank spawning procedures that allow for a high level of genetic diversity to be retained among the offspring. The pace-set method reduces hormone use and may be applied to commercial striped bass production as well as captive spawning of other fish species.}, journal={AQUACULTURE}, author={Andersen, L. K. and Clark, R. W. and McGinty, A. S. and Hopper, M. S. and Kenter, L. W. and Salger, S. A. and Schilling, J. and Hodson, R. G. and Kovach, A. I. and Berlinsky, D. L. and et al.}, year={2021}, month={Feb} }
 @article{borski_hodson_2003, title={Fish research and the institutional animal care and use committee}, volume={44}, ISSN={["1930-6180"]}, DOI={10.1093/ilar.44.4.286}, abstractNote={Fish represent the most diverse group of animals in the vertebrate phylum. The more than 25,000 species are characterized by an array of anatomical, biochemical, physiological, and behavioral repertoires. For this reason, it is difficult to develop a comprehensive guideline on the care and use of fishes. Institutional animal care and use committees (IACUCs) meet the challenge of ensuring adequate fish welfare using guidelines (Animal Welfare Act [AWA] and Public Health Service [PHS] Policy and their guides) derived mainly from the care and use of mammalian species, which may not be optimal for regulating fish research, teaching, or extension activities. Discussion focuses on various issues that often confront IACUCs in meeting regulatory requirements while assuring proper fish welfare. Issues include questions concerning animal tracking and inventory, utilization of fisheries bycatch, facility inspections in remote locations, and euthanasia. Common sense solutions appropriate for field and laboratory fish activities are suggested, which should help investigators, IACUCs, and regulatory agencies meet PHS and AWA objectives.}, number={4}, journal={ILAR JOURNAL}, author={Borski, RJ and Hodson, RG}, year={2003}, pages={286–294} }
 @article{weber_king_clark_hodson_sullivan_2000, title={Morpho-physiological predictors of ovulatory success in captive striped bass (Morone saxatilis)}, volume={188}, ISSN={["1873-5622"]}, DOI={10.1016/S0044-8486(00)00328-8}, abstractNote={This study evaluates morpho-physiological characters as predictors of ovulatory success in cultured striped bass, Morone saxatilis, that could be used by farmers to select females for induced spawning. Diameter, size homogeneity and growth of ovarian follicles; blood plasma levels of testosterone (T), oestradiol-17β (E2) and vitellogenin (VTG); and in vitro maturation of oocytes, in response to a combination of insulin-like growth factor-I (IGF-I, 100 nM) and 17,20β,21-trihydroxy-4-pregnen-3-one (20β-S, 290 nM) were examined for females prior to spawning induction and compared with their subsequent ovulatory response. Fish spawning within 8 days of implantation with pelleted analogue of mammalian gonadotropin-releasing hormone analogue (GnRHa; [d-Ala6-des-Gly10-NEt]-LHRH) were considered to have given a satisfactory maturational response. The in vitro assay was the most reliable predictor for ovulatory success. All fish whose oocytes completed final oocyte maturation (FOM) in vitro in response to the combination of IGF-I and 20β-S spawned, whereas, 12 out of 13 fish, whose oocytes did not complete FOM in vitro, failed to spawn within 8 days of GnRHa treatment. The in vitro assay was field-tested on commercial farms, and correctly identified all four females that spawned out of the eight females that were given hormone treatment. Among the other measurements, follicle diameter best differentiated between fish that later spawned and those that did not spawn. Plasma T concentrations were greater on average in fish that spawned, but the technical complexity of the assay and overlap in T concentrations between fish that spawned and those that did not limits the value of this measurement to farmers. There was no significant difference in follicle size homogeneity, follicle growth over the 2-week period prior to hormone treatment, or plasma levels of E2 and VTG between fish that spawned and those that did not.}, number={1-2}, journal={AQUACULTURE}, author={Weber, GM and King, W and Clark, RW and Hodson, RG and Sullivan, CV}, year={2000}, month={Aug}, pages={133–146} }
 @article{daniels_hodson_1999, title={Weaning success of southern flounder juveniles: Effect of changeover period and diet type on growth and survival}, volume={61}, ISSN={["1522-2055"]}, DOI={10.1577/1548-8454(1999)061<0047:WSOSFJ>2.0.CO;2}, abstractNote={Abstract Two studies were conducted to determine the effects of changeover period duration and diet type (dry versus semimoist) on growth and survival of postmetamorphic southern flounder Paralichthys lethostigma during weaning from live feed onto formulated feeds. In the first study, feeding with live brine shrimp Artemia sp. was abruptly stopped after changeover periods of 5, 10, 15, and 20 d. A changeover period of 20 d resulted in significantly higher (P 0.05) from the other treatments or the control (live brine shrimp). In the second study, feeding with live brine shrimp was gradually reduced from 100% to 0% over a 12-d changeover period. Brine shrimp were replaced with either a dry feed or a semimoist feed. Survival of fish fed either the dry feed (71.1 ± 30.1%) or the semimoist feed (71.1 ± 15.4%) was not si...}, number={1}, journal={NORTH AMERICAN JOURNAL OF AQUACULTURE}, author={Daniels, HV and Hodson, RG}, year={1999}, month={Jan}, pages={47–50} }
 @article{mcmurtry_sanders_cure_hodson_haning_st amand_1997, title={Efficiency of water use of an integrated fish/vegetable co-culture system}, volume={28}, ISSN={["0893-8849"]}, DOI={10.1111/j.1749-7345.1997.tb00290.x}, abstractNote={Abstract.– Fish and vegetable production were linked in a recirculating water system designed to achieve a high degree of efficiency of water use for food production in addition to functional and technological simplicity. Hybrid tilapia Oreochromis mossambicus×O. niloticus L. were grown in tanks associated with biofilters (sand beds) in which tomatoes Lycopersicon esculentum were grown. The effect of four biofilter volume (BFV)/fish rearing tank volume ratios (0.67/1, 1.00/1, 1.5011, 2.25/1) on water use efficiency was evaluated.‘Laura’(first experiment) or‘Kewalo’tomatoes were grown 4/m2 in biofilters of four different sizes and surface‐irrigated 8 times daily with water from the associated fish tanks. Daily water consumption increased with BFV/tank ratios and with time. Fish production rates increased with biofilter volume in the first experiment, but were not significantly different in the second experiment. Total tomato fruit yield per plot increased from 13.7 to 31.7 kg (Experiment 1) and from 19.9 to 33.1 kg (Experiment 2) with increasing BFV/tank ratio. For fish plus fruit, total energy production increased from 4,950 to 8,963 kcal/ plot and from 4,804 to 7,424 kcal/plot in Experiments 1 and 2, respectively, and protein production increased from 536 to 794 and from 352 to 483 g/plot in Experiments 1 and 2, respectively, with increasing BFV/ tank ratio. Trends in water use efficiency for production of food energy (kcal/L.) and of protein (g/L) in tomatoes and fish were complex. Water use efficiency}, number={4}, journal={JOURNAL OF THE WORLD AQUACULTURE SOCIETY}, author={McMurtry, MR and Sanders, DC and Cure, JD and Hodson, RG and Haning, BC and St Amand, PC}, year={1997}, month={Dec}, pages={420–428} }
 @article{berlinsky_william_hodson_sullivan_1997, title={Hormone induced spawning of summer flounder Paralichthys dentatus}, volume={28}, ISSN={["0893-8849"]}, DOI={10.1111/j.1749-7345.1997.tb00964.x}, abstractNote={Abstract}, number={1}, journal={JOURNAL OF THE WORLD AQUACULTURE SOCIETY}, author={Berlinsky, DL and William, K and Hodson, RG and Sullivan, CV}, year={1997}, month={Mar}, pages={79–86} }
 @inbook{sullivan_berlinsky_hodson_1997, title={Reproduction}, DOI={10.1016/s0167-9309(97)80004-x}, booktitle={Striped bass and other morone culture  (Developments in aquaculture and fisheries science;  30)}, publisher={New York: Elsevier}, author={Sullivan, C. V. and Berlinsky, D. L. and Hodson, R. G.}, year={1997}, pages={11–73} }
 @article{hodson_sullivan_1993, title={Induced maturation and spawning of domestic and wild striped bass, Morone saxatilis (Walbaum), broodstock with implanted GnRH analogue and injected hCG}, volume={24}, DOI={10.1111/j.1365-2109.1993.tb00562.x}, abstractNote={Abstract. A domestic striped bass. Morone saxatilis (Walbaum), broodstock was established by rearing fish to sexual maturity in ponds. A method was developed to reproduce the domestic females, and also wild females too immature to be successfully induced to spawn with injected human chorionic gonadotropin (hCG). The fish were implanted with pellets containing 100–150μg of a synthetic analogue of mammalian gonadotropin reieasing-hormone, [D-Ala6- Pro9-NEt]-LHRH (mGnRHa), in a matrix of cholesterol (CH) and cellulose. They were implanted with one fast hormone-release (80% CH) pellet and one slow hormone-release (95% CH) pellet and allowed to mature for 1–3 days, until they entered the process of final oocyte maturation and were induced to ovulate or spawn with an hCG injection. The secondary hCG injection was found to be necessary to speed the maturation of the wild fish; they otherwise would succumb to the stresses of capture, handling and confinement before they could be spawned. The total mGnRHa dosages used ranged from 33 to 111μg mGnRHa/kg body weight, and the hCG doses were either 165 or 330 IU hCG/kg body weight. Using the combined mGnRHa implant-hCG injection technique, fry production rates were comparable to those obtained using fully mature wild females taken directly from their spawning grounds.}, number={3}, journal={Aquaculture and Fisheries Management}, author={Hodson, R. G. and Sullivan, C. V.}, year={1993}, pages={389} }