Rebecca-Ayme Hughes

Works (4)

Updated: August 18th, 2023 21:15

2019 journal article

More Is Not Always Better: Evaluation of 1D and 2D-LC-MS/MS Methods for Metaproteomics

FRONTIERS IN MICROBIOLOGY, 10.

By: T. Hinzke *, A. Kouris *, R. Hughes n, M. Strous * & M. Kleiner n 

author keywords: microbiota; microbiome; mock community; method evaluation; microbial ecology; Q Exactive; liquid chromatography; GeLC
TL;DR: It is shown here that, while 1D-LC approaches are faster and easier to set up and lead to more identifications per minute of runtime, 2D- LC approaches allow for a higher overall number of identifications with up to >10,000 protein groups identified. (via Semantic Scholar)
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15. Life on Land (OpenAlex)
Sources: Web Of Science, NC State University Libraries
Added: March 4, 2019

2018 journal article

Genome sequence, metabolic properties and cyanobacterial attachment of Porphyrobacter sp. HT-58-2 isolated from a filamentous cyanobacterium–microbial consortium

Microbiology, 164(10), 1229–1239.

By: R. Hughes n, X. Jin n, Y. Zhang n, R. Zhang n, S. Tran n, P. Williams *, J. Lindsey n , E. Miller n 

author keywords: alphaproteobacteria; fluorescence in situ hybridization (FISH); tolyporphins; bacteriochlorin; photosynthetic gene cluster
MeSH headings : Alphaproteobacteria / classification; Alphaproteobacteria / genetics; Alphaproteobacteria / metabolism; Alphaproteobacteria / physiology; Bacterial Proteins / genetics; Base Sequence; Chromosome Mapping; Cyanobacteria / metabolism; DNA, Bacterial / genetics; Genome, Bacterial / genetics; In Situ Hybridization, Fluorescence; Microbial Consortia; Phylogeny; RNA, Ribosomal, 16S / genetics; Sequence Analysis, DNA
TL;DR: The studies reported herein expand the description of the community of predominantly alphaproteobacteria associated with the filamentous HT- 58-2 cyanobacterium and isolate a dominant bacterium, Porphyrobacter sp. (via Semantic Scholar)
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14. Life Below Water (OpenAlex)
15. Life on Land (Web of Science)
Sources: Web Of Science, NC State University Libraries, Crossref
Added: January 7, 2019

2017 journal article

Genome Sequence and Composition of a Tolyporphin-Producing Cyanobacterium-Microbial Community

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 83(19).

By: R. Hughes n, Y. Zhang n, R. Zhang n, P. Williams *, J. Lindsey n  & E. Miller n 

author keywords: cyanobacteria; genome analysis; phylogenetic analysis; tetrapyrroles; tolyporphin
MeSH headings : Bacterial Proteins / genetics; Bacterial Proteins / metabolism; Biosynthetic Pathways; Cyanobacteria / chemistry; Cyanobacteria / genetics; Cyanobacteria / growth & development; Cyanobacteria / metabolism; Genome, Bacterial; Metagenomics; Multigene Family; Phylogeny; Porphyrins / biosynthesis; Porphyrins / chemistry
TL;DR: It is found that the HT-58-2 culture is composed of the cyanobacterium and a community of associated bacteria, complicating the question of which organisms make tolyporphins, and an intriguing gene cluster that contains tetrapyrrole biosynthesis genes and a collection of unknown genes suggests a possible role in toly porphin biosynthesis. (via Semantic Scholar)
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Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2017 journal article

Quantitation of Tolyporphins, Diverse Tetrapyrrole Secondary Metabolites with Chlorophyll-Like Absorption, from a Filamentous Cyanobacterium-Microbial Community

Phytochemical Analysis, 29(2), 205–216.

By: Y. Zhang n, R. Zhang n, R. Hughes n, J. Dai *, J. Gurr*, P. Williams *, E. Miller n , J. Lindsey n 

author keywords: HPLC; mass spectrometry; absorption spectroscopy with multicomponent analysis; nitrogen deprivation; growth curve
MeSH headings : Chlorophyll / chemistry; Chlorophyll A; Chromatography, High Pressure Liquid / methods; Cyanobacteria / growth & development; Cyanobacteria / metabolism; Porphyrins / analysis; Porphyrins / chemistry; Reference Standards; Reproducibility of Results; Spectrum Analysis / methods; Tetrapyrroles / analysis
TL;DR: The quantitative methods developed herein should facilitate investigation of the biosynthesis of tolyporphins (and other tetrapyrroles) as well as examination of other strains for production of tolporph Ins. (via Semantic Scholar)
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Sources: Web Of Science, NC State University Libraries, Crossref
Added: August 6, 2018

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