@misc{woodrow_sheats_cooper_bayless_2023, title={Asthma: The Use of Animal Models and Their Translational Utility}, volume={12}, ISSN={["2073-4409"]}, url={https://doi.org/10.3390/cells12071091}, DOI={10.3390/cells12071091}, abstractNote={Asthma is characterized by chronic lower airway inflammation that results in airway remodeling, which can lead to a permanent decrease in lung function. The pathophysiology driving the development of asthma is complex and heterogenous. Animal models have been and continue to be essential for the discovery of molecular pathways driving the pathophysiology of asthma and novel therapeutic approaches. Animal models of asthma may be induced or naturally occurring. Species used to study asthma include mouse, rat, guinea pig, cat, dog, sheep, horse, and nonhuman primate. Some of the aspects to consider when evaluating any of these asthma models are cost, labor, reagent availability, regulatory burden, relevance to natural disease in humans, type of lower airway inflammation, biological samples available for testing, and ultimately whether the model can answer the research question(s). This review aims to discuss the animal models most available for asthma investigation, with an emphasis on describing the inciting antigen/allergen, inflammatory response induced, and its translation to human asthma.}, number={7}, journal={CELLS}, author={Woodrow, Jane Seymour and Sheats, M. Katie and Cooper, Bethanie and Bayless, Rosemary}, year={2023}, month={Apr} }
 @article{bayless_cooper_sheats_2022, title={Investigation of plasma cell-free DNA as a potential biomarker in horses}, volume={34}, ISSN={["1943-4936"]}, url={https://doi.org/10.1177/10406387221078047}, DOI={10.1177/10406387221078047}, abstractNote={ Plasma cell-free DNA (cfDNA) is a biomarker of ischemia, systemic inflammation, and mortality in humans with gastrointestinal disease. Cell-free DNA has not been investigated as a biomarker for equine colic, to our knowledge. We hypothesized that cfDNA could be measured accurately in neat equine plasma using a benchtop fluorometer and that plasma cfDNA would be elevated in emergency patients compared to healthy horses. Plasma was obtained from blood collected in Roche DNA stabilizing tubes. We used the Qubit 4 fluorometer and 1× dsDNA HS assay kit to measure cfDNA concentration in neat patient plasma and following DNA extraction of plasma with a commercial kit. Assay precision and linearity of dilution were satisfactory for neat plasma cfDNA, but DNA spike and recovery results were variable. Further, cfDNA concentrations in paired neat plasma and extracted-plasma samples ( n = 66) were not correlated. Median extracted-plasma cfDNA was higher in emergency patients ( n = 50) and a subgroup of colic patients ( n = 36), compared to healthy horses ( n = 19). Our results with extracted-plasma samples provide proof of concept for further investigation of plasma cfDNA as a biomarker in horses. }, number={3}, journal={JOURNAL OF VETERINARY DIAGNOSTIC INVESTIGATION}, publisher={SAGE Publications}, author={Bayless, Rosemary L. and Cooper, Bethanie L. and Sheats, M. Katie}, year={2022}, month={Feb} }
 @article{bayless_bayless_sheats_jones_2022, title={Withaferin A Inhibits Neutrophil Adhesion, Migration, and Respiratory Burst and Promotes Timely Neutrophil Apoptosis}, volume={9}, ISSN={["2297-1769"]}, url={https://europepmc.org/articles/PMC9247543}, DOI={10.3389/fvets.2022.900453}, abstractNote={Neutrophils play a major role in many equine conditions, including equine asthma, laminitis, and intestinal ischemia and reperfusion injury, and therefore represent an attractive target for innovative therapeutic approaches. Novel strategies for reducing neutrophilic inflammation include modulation of neutrophil functions and lifespan. Withaferin A (WFA) is a phytochemical with well-establishedin vitroandin vivoanti-inflammatory properties, but its direct effects on neutrophils are largely unknown. We hypothesized that WFA would inhibit adhesion, migration, and respiratory burst by equine neutrophils and promote timely apoptosis of primed equine neutrophils. Consistent with this hypothesis, our data show that WFA causes a significant, concentration-dependent inhibition of equine neutrophil adhesion, migration, and respiratory burst in response to diverse stimuli. Further, WFA treatment increased apoptosis of equine neutrophils exposed to GM-CSF for 24 h. This pro-apoptotic effect of WFA was not observed in unprimed neutrophils, nor at the 2-h time point relevant to our functional neutrophil experiments. Our data demonstrate that WFA may reduce neutrophil-mediated inflammation through multiple mechanisms, including suppression of inflammatory responses and promotion of apoptosis. Additional research is needed to elucidate the molecular mechanisms for these effects and evaluate the potential clinical use of WFA in veterinary and human patients.}, journal={FRONTIERS IN VETERINARY SCIENCE}, author={Bayless, Rosemary and Bayless, RL and Sheats, M. Katie and Jones, Sam}, editor={Bayless, RosemaryEditor}, year={2022}, month={Jun} }
 @article{pezzanite_easley_bayless_aldrich_nelson_seim_nout-lomas_2021, title={Outcomes after cervical vertebral interbody fusion using an interbody fusion device and polyaxial pedicle screw and rod construct in 10 horses (2015-2019)}, volume={5}, ISSN={["2042-3306"]}, url={https://doi.org/10.1111/evj.13449}, DOI={10.1111/evj.13449}, abstractNote={Abstract}, journal={EQUINE VETERINARY JOURNAL}, author={Pezzanite, Lynn M. and Easley, Jeremiah T. and Bayless, Rosemary and Aldrich, Ellison and Nelson, Brad B. and Seim, Howard B., III and Nout-Lomas, Yvette S.}, year={2021}, month={May} }
 @article{bayless_moore_hassel_byer_landolt_nout-lomas_2019, title={Equine urinary N-acetyl-beta-D-glucosaminidase assay validation and correlation with other markers of kidney injury}, volume={31}, ISSN={["1943-4936"]}, url={https://doi.org/10.1177/1040638719867124}, DOI={10.1177/1040638719867124}, abstractNote={Detection of equine acute kidney injury (AKI) is hindered by limited markers of early renal damage in horses. N-acetyl-β-D-glucosaminidase (NAG), a lysosomal enzyme in renal tubular epithelium released into urine during tubular insult, has shown promise for early identification of AKI in humans and other species. We validated an assay for NAG in equine urine and measured urinary NAG in 7 azotemic and 7 non-azotemic client-owned adult horses. The enzymatic NAG assay was validated using within- and between-run coefficients of variation (CVs), recovery following standard addition, and linearity of dilution. Intra- and inter-run CVs (21% and 3.2%, respectively), average recovery following standard addition (99–109%), and linearity under serial dilution ( R2= 0.997) were satisfactory. Urine NAG index was significantly correlated with urinary fractional excretion of sodium (FENa; ρ = 0.76, p < 0.001) and plasma creatinine (ρ = 0.74, p = 0.001). Median urine NAG indices were higher in azotemic horses ( p = 0.006), in horses with increased urinary FENa( p = 0.006), and in horses with increased urine gamma-glutamyl transferase index ( p = 0.032). Urine NAG can be measured in horses and shows positive correlation with 2 current renal biomarkers. Additional work is needed to establish normal equine reference intervals and characterize the increase of urine NAG index in horses in relation to tubular injury.}, number={5}, journal={JOURNAL OF VETERINARY DIAGNOSTIC INVESTIGATION}, publisher={SAGE Publications}, author={Bayless, Rosemary L. and Moore, A. Russell and Hassel, Diana M. and Byer, Brittney J. and Landolt, Gabriele A. and Nout-Lomas, Yvette S.}, year={2019}, month={Sep}, pages={688–695} }