Works (338)

Updated: April 3rd, 2024 19:55

2024 article

Complete genome sequence for the extremely thermophilic bacterium Anaerocellum danielii (DSM:8977)

Manesh, M. J. H., Bing, R. G., Willard, D. J., Adams, M. W. W., & Kelly, R. M. (2024, January 24). (K. M. Stedman, Ed.). MICROBIOLOGY RESOURCE ANNOUNCEMENTS, Vol. 1.

By: M. Manesh n, R. Bing n, D. Willard n, M. Adams* & R. Kelly n

Ed(s): K. Stedman

author keywords: Anaerocellum danielii; extreme thermophile; cellulose
TL;DR: The complete genome sequence of the extremely thermophilic bacterium Anaerocellum Anaerocellum danielii (DSM:8977) is reported here and is capable of lignocellulose degradation with potential applications in biomass degradation and production of chemicals and fuels from renewable feedstocks. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries, ORCID
Added: February 12, 2024

2024 article

Complete genome sequence for the thermoacidophilic archaeon <i>Metallosphaera sedula</i> (DSM:5348)

Manesh, M. J. H., Bing, R. G., Willard, D. J., & Kelly, R. M. (2024, February 8). MICROBIOLOGY RESOURCE ANNOUNCEMENTS, Vol. 2.

By: M. Manesh n, R. Bing n, D. Willard n & R. Kelly n

author keywords: Metallosphaera sedula; thermoacidophile; archeaon
TL;DR: The complete genome sequence of the thermoacidophilic archaeon Metallosphaera sedula, originally isolated from a volcanic field in Italy, is reported here, and has applications in bioleaching of sulfide minerals. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: February 19, 2024

2024 review

Extremely thermoacidophilic archaea for metal bioleaching:<i> What</i><i> do</i><i> their</i> genomes tell Us?

[Review of ]. BIORESOURCE TECHNOLOGY, 391.

By: M. Manesh n, D. Willard n, A. Lewis n & R. Kelly n

author keywords: Archaea; Sulfolobales; Metal biooxidation; Sulfur biooxidation; Chalcopyrite; High temperatures
TL;DR: The evolving landscape for bioleaching technologies at high temperatures merits a closer look through a genomic lens at what is currently possible and what lies ahead in terms of new developments and emerging opportunities. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: December 18, 2023

2023 article

Complete Genome Sequences of Caldicellulosiruptor acetigenus DSM 7040, Caldicellulosiruptor morganii DSM 8990 (RT8.B8), and Caldicellulosiruptor naganoensis DSM 8991 (NA10)

Bing, R. G. G., Willard, D. J. J., Manesh, M. J. H., Laemthong, T., Crosby, J. R. R., Adams, M. W. W., & Kelly, R. M. M. (2023, February 1). MICROBIOLOGY RESOURCE ANNOUNCEMENTS, Vol. 2.

TL;DR: The genome sequences of three extremely thermophilic, lignocellulolytic Caldicellulosiruptor species were closed, improving previously reported multiple-contig assemblies and enhancing bioinformatic analysis of the species. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: February 20, 2023

2023 journal article

Complete Genome Sequences of Two Thermophilic Indigenous Bacteria Isolated from Wheat Straw, Thermoclostridium stercorarium subsp. Strain RKWS1 and Thermoanaerobacter sp. Strain RKWS2

MICROBIOLOGY RESOURCE ANNOUNCEMENTS, 12(3).

TL;DR: Discovery of indigenous thermophiles in plant biomass suggests that high-temperature microorganisms are more ubiquitous than previously thought. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: April 17, 2023

2023 article

Complete genome sequence for the thermoacidophilic archaeon <i>Sulfuracidifex</i> (f<i>. Sulfolobus</i>) <i>metallicus</i> DSM 6482

Willard, D. J., Manesh, M. J. H., Bing, R. G., & Kelly, R. M. (2023, December 6). MICROBIOLOGY RESOURCE ANNOUNCEMENTS, Vol. 12.

By: D. Willard n, M. Manesh n, R. Bing n & R. Kelly n

author keywords: thermoacidophile; archaea; crenarchaea; genome
TL;DR: The complete genome sequence for the thermoacidophilic archaeon Sulfuracidifex (f. Sulfolobus) metallicus DSM 6482 is reported, which is a prolific metal and sulfur oxidizer and has application in metal bioleaching operations. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: December 18, 2023

2023 journal article

Fermentative conversion of unpretreated plant biomass: A thermophilic threshold for indigenous microbial growth

BIORESOURCE TECHNOLOGY, 367.

By: R. Bing n, M. Carey n, T. Laemthong n, D. Willard n, J. Crosby n, D. Sulis n, J. Wang n, M. Adams*, R. Kelly n

author keywords: Thermophiles; Lignocellulose; Consolidated bioprocessing; Caldicellulosiruptor bescii; Acetivibrio thermocellus
MeSH headings : Biomass; Fermentation; Lignin / chemistry; Clostridium thermocellum / metabolism; Plants / metabolism
TL;DR: Thermophiles that naturally grow at and above 75°C offer specific advantages as platform microorganisms for biomass conversion into fuels and chemicals that prevents native microbes from competing with engineered microbes and forming undesirable by-products. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries, ORCID
Added: January 17, 2023

2023 article

Innovating Life Sciences Laboratory Training: Molecular Biology Laboratory Education Modules (MBLEMs) as a Model for Advanced Training at Diverse Institutions

JOURNAL OF BIOLOGICAL CHEMISTRY, Vol. 299, pp. S260–S260.

By: M. Srougi*, A. Corbett, C. Garcia, M. Sabaoun, M. Santisteban, V. Sivaraman, S. Chen*, C. Goller*, R. Kelly*

Sources: Web Of Science, NC State University Libraries
Added: September 11, 2023

2023 article

Interplay between transcriptional regulators and VapBC toxin-antitoxin loci during thermal stress response in extremely thermoacidophilic archaea

Cooper, C. R., Lewis, A. M., Notey, J. S., Mukherjee, A., Willard, D. J., Blum, P. H., & Kelly, R. M. (2023, February 24). ENVIRONMENTAL MICROBIOLOGY, Vol. 2.

By: C. Cooper n, A. Lewis n, J. Notey n, A. Mukherjee n, D. Willard n, P. Blum*, R. Kelly n

MeSH headings : Antitoxins / genetics; Toxins, Biological / genetics; Toxins, Biological / metabolism; Heat-Shock Response / genetics; Sulfolobus solfataricus / genetics; Sulfolobus solfataricus / metabolism; Escherichia coli / genetics
TL;DR: The interplay between VapBC TA loci and heat shock regulators in Sa. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Sources: Web Of Science, NC State University Libraries
Added: March 13, 2023

2023 article

Manipulating Fermentation Pathways in the Hyperthermophilic Archaeon Pyrococcus furiosus for Ethanol Production up to 95 degrees C Driven by Carbon Monoxide Oxidation

Lipscomb, G. L., Crowley, A. T., Nguyen, D. M. N., Keller, M. W., Hailey C. O'Quinn, Tanwee, T. N. N., … Adams, M. W. W. (2023, May 10). APPLIED AND ENVIRONMENTAL MICROBIOLOGY, Vol. 5.

By: G. Lipscomb*, A. Crowley*, D. Nguyen*, M. Keller*, . Hailey C. O'Quinn*, T. Tanwee*, J. Vailionis*, K. Zhang* ...

author keywords: Pyrococcus furiosus; archaea; thermophile; ethanol; genetic engineering; CO dehydrogenase; bioethanol; carbon monoxide; hyperthermophiles; metabolic engineering; metabolic modeling
TL;DR: An engineered strain of P. furiosus is described that synthesizes ethanol at 95°C, utilizing a homologously-expressed native alcohol dehydrogenase, termed AdhF, and opens the door to the potential for ‘bioreactive distillation’ since fermentation can be performed well above the normal boiling point of ethanol. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Sources: Web Of Science, NC State University Libraries
Added: May 22, 2023

2023 article

Metabolic engineering of <i>Caldicellulosiruptor bescii</i> for 2,3-butanediol production from unpretreated lignocellulosic biomass and metabolic strategies for improving yields and titers

Tanwee, T. N. N., Lipscomb, G. L., Vailionis, J. L., Zhang, K., Bing, R. G., Hailey C. O'Quinn, … Adams, M. W. W. (2023, December 22). APPLIED AND ENVIRONMENTAL MICROBIOLOGY, Vol. 12.

By: T. Tanwee*, G. Lipscomb*, J. Vailionis*, K. Zhang*, R. Bing n, . Hailey C. O'Quinn*, F. Poole*, Y. Zhang*, R. Kelly n, M. Adams*

author keywords: Caldicellulosiruptor bescii; thermophile; 2,3-butanediol; acetoin; lignocellulose; metabolic flux; redox balance
Sources: Web Of Science, NC State University Libraries
Added: January 8, 2024

2023 journal article

Multiplex CRISPR editing of wood for sustainable fiber production

SCIENCE, 381(6654), 216-+.

By: D. Sulis n, X. Jiang n, C. Yang n, B. Marques n, M. Matthews n, Z. Miller n, K. Lan n, C. Cofre-Vega n ...

TL;DR: This work demonstrates that genome editing can be harnessed for breeding more efficient trees, which will provide timely opportunities for sustainable forestry and a more efficient bioeconomy. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Sources: Web Of Science, NC State University Libraries, ORCID
Added: August 9, 2023

2023 article

Optimizing Strategies for Bio-Based Ethanol Production Using Genome-Scale Metabolic Modeling of the Hyperthermophilic Archaeon, Pyrococcus furiosus

Vailionis, J. L., Zhao, W., Zhang, K., Rodionov, D. A., Lipscomb, G. L., Tanwee, T. N. N., … Zhang, Y. (2023, June 8). APPLIED AND ENVIRONMENTAL MICROBIOLOGY, Vol. 6.

By: J. Vailionis*, W. Zhao*, K. Zhang*, D. Rodionov*, G. Lipscomb*, T. Tanwee*, . Hailey C. O'Quinn*, R. Bing n ...

author keywords: Pyrococcus furiosus; thermophiles; ethanol; genome-scale modeling; CO dehydrogenase; Archaea
TL;DR: The P. furiosus model is a powerful tool for understanding the relationship between generation of end products and redox/energy balance at a systems-level that will aid in the design of optimal engineering strategies for production of bio-based chemicals and fuels. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Sources: Web Of Science, NC State University Libraries
Added: June 19, 2023

2023 journal article

Role of cell-substrate association during plant biomass solubilization by the extreme thermophile Caldicellulosiruptor bescii

EXTREMOPHILES, 27(1).

By: T. Laemthong n, R. Bing n, J. Crosby n, M. Manesh n, M. Adams* & R. Kelly n

author keywords: Caldicellulosiruptor; Extreme thermophile; Lignocellulose; Binding proteins; Tapirins
MeSH headings : Cellulose / metabolism; Biomass; Saccharum / metabolism; Caldicellulosiruptor / metabolism; Clostridiales / metabolism; Plants; Archaea / metabolism
TL;DR: Results show that enhanced binding to the substrate, beyond the native capability, did not improve C. bescii solubilization of plant biomass, but in some cases may improve conversion of released lignocellulose carbohydrates to fermentation products. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: March 13, 2023

2023 article

Stay or Go: Sulfolobales Biofilm Dispersal Is Dependent on a Bifunctional VapB Antitoxin

Lewis, A. M., Willard, D. J., Manesh, M. J. H. J., Sivabalasarma, S., Albers, S.-V., & Kelly, R. M. (2023, April 10). MBIO, Vol. 4.

author keywords: toxin-antitoxin loci; biofilms; Sulfolobales; thermoacidophiles; Sulfolobus acidocaldarius
MeSH headings : Antitoxins / metabolism; Bacterial Proteins / metabolism; Bacterial Toxins / metabolism; Sulfolobales; Biofilms
TL;DR: The findings here suggest that a stand-alone VapB-type antitoxin was the product of selective evolutionary pressure to influence biofilm formation in these archaea, a vital microbial community behavior. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: May 9, 2023

2023 journal article

Whither the genus Caldicellulosiruptor and the order Thermoanaerobacterales: phylogeny, taxonomy, ecology, and phenotype

FRONTIERS IN MICROBIOLOGY, 14.

By: R. Bing n, D. Willard n, J. Crosby n, M. Adams* & R. Kelly n

author keywords: Caldicellulosiruptor; Thermoanaerobacterales; bacteria; thermophiles; phylogeny; ecology; fermentative anaerobes
TL;DR: A main outcome of ANI/AAI analysis indicates the need to reclassify several previously designated species in the Thermoanaerobacterales and Caldicellulosiruptorales by condensing them into strains of single species. (via Semantic Scholar)
UN Sustainable Development Goal Categories
14. Life Below Water (OpenAlex)
Sources: Web Of Science, NC State University Libraries
Added: August 28, 2023

2022 journal article

<p>Plant biomass fermentation by the extreme thermophile Caldicellulosiruptor bescii for co-production of green hydrogen and acetone: Technoeconomic analysis</p>

BIORESOURCE TECHNOLOGY, 348.

By: R. Bing n, C. Straub n, D. Sulis n, J. Wang n, M. Adams* & R. Kelly n

author keywords: Acetone; Green hydrogen; Caldicellulosiruptor bescii; Extreme thermophiles; Technoeconomic analysis
MeSH headings : Acetone; Biomass; Caldicellulosiruptor; Fermentation; Hydrogen; Lignin / chemistry
Sources: Web Of Science, NC State University Libraries, ORCID
Added: April 25, 2022

2022 article

Biochemical and Regulatory Analyses of Xylanolytic Regulons in Caldicellulosiruptor bescii Reveal Genus-Wide Features of Hemicellulose Utilization

Crosby, J. R., Laemthong, T., Bing, R. G., Zhang, K., Tanwee, T. N. N., Lipscomb, G. L., … Kelly, R. M. (2022, October 11). APPLIED AND ENVIRONMENTAL MICROBIOLOGY, Vol. 10.

author keywords: Caldicellulosiruptor; extreme thermophile; hemicellulose; xylanases; hemicellulase
MeSH headings : Regulon; Xylans; Cellulose / metabolism; Clostridiales / metabolism; Sugars
TL;DR: Overall, this work demonstrates the essential requirements for Caldicellulosiruptor to degrade various forms of xylan and the differences in species genomic inventories that are tuned for survival in unique biotopes with variable lignocellulosic substrates. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Sources: Web Of Science, NC State University Libraries
Added: October 24, 2022

2022 article

Engineering Caldicellulosiruptor bescii with Surface Layer Homology Domain-Linked Glycoside Hydrolases Improves Plant Biomass Solubilization

Laemthong, T., Bing, R. G., Crosby, J. R., Adams, M. W. W., & Kelly, R. M. (2022, September 28). APPLIED AND ENVIRONMENTAL MICROBIOLOGY, Vol. 9.

By: T. Laemthong n, R. Bing n, J. Crosby n, M. Adams* & R. Kelly n

author keywords: Caldicellulosiruptor; lignocellulose; glycoside hydrolase; surface layer homology (SLH) domain; plant biomass degradation; surface layer homology domain
MeSH headings : Glycoside Hydrolases / metabolism; Biomass; Xylans / metabolism; Xylose; Clostridiales / metabolism; Cellulose / metabolism; Plants / microbiology; Populus
UN Sustainable Development Goal Categories
Sources: Web Of Science, NC State University Libraries
Added: October 11, 2022

2021 journal article

MeSH headings : Air Microbiology; Animals; Archaea / classification; Archaea / genetics; Archaea / isolation & purification; Bacteria / classification; Bacteria / genetics; Bacteria / isolation & purification; Catalogs as Topic; Ecosystem; Humans; Metabolomics / methods; Metagenome; Metagenomics / methods; Phylogeny; Soil Microbiology; Viruses / genetics; Viruses / isolation & purification; Water Microbiology
TL;DR: The utility of this collection of >10,000 metagenomes collected from diverse habitats covering all of Earth’s continents and oceans is demonstrated for understanding secondary-metabolite biosynthetic potential and for resolving thousands of new host linkages to uncultivated viruses. (via Semantic Scholar)
UN Sustainable Development Goal Categories
14. Life Below Water (OpenAlex)
Sources: Web Of Science, NC State University Libraries
Added: July 27, 2021

2021 chapter

Enzymes from extremely thermophilic bacteria and archaea: Current status and future prospects

In N. A. Arora, S. Agnihotri, & J. Mishra (Eds.), Extremozymes and their Industrial Applications. St. Louis, MO: Elsevier Academic Press.

By: T. Laemthong, A. Lewis, J. Crosby, R. Bing, W. Schneider, D. Willard, J. Counts, R. Kelly

Ed(s): N. Arora, S. Agnihotri & J. Mishra

Source: NC State University Libraries
Added: July 27, 2021

2021 article

Fox Cluster determinants for iron biooxidation in the extremely thermoacidophilic Sulfolobaceae

Counts, J. A., Vitko, N. P., & Kelly, R. M. (2021, August 30). ENVIRONMENTAL MICROBIOLOGY, Vol. 8.

By: J. Counts n, N. Vitko n & R. Kelly n

MeSH headings : Animals; Archaeal Proteins / genetics; Archaeal Proteins / metabolism; Iron / metabolism; Phylogeny; Proteomics; Sulfolobaceae / genetics
TL;DR: Proteomic analysis here of iron-oxidizing membranes from M. sedula indicates that FoxA2 and FoxB and FoxC are essential and few homologs exist for FoxC or for most other Fox Cluster proteins. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: September 7, 2021

2021 journal article

Genome-Scale Metabolic Model of Caldicellulosiruptor bescii Reveals Optimal Metabolic Engineering Strategies for Bio-based Chemical Production

MSystems, 6(3).

By: D. Rodionov*, I. Rodionova, V. Rodionov, A. Arzamasov, K. Zhang*, G. Rubinstein*, T. Tanwee*, R. Bing n ...

Ed(s): Z. Summers

author keywords: Caldicellulosiruptor bescii; lignocellulose degradation; carbohydrate; Caldicellulosiruptor; carbohydrate utilization; comparative genomics; metabolic reconstruction; plant biomass degradation; regulon
TL;DR: The C. bescii model provides a useful platform for investigating the potential redox controls that mediate the carbon and energy flows in metabolism and sets the stage for future design of engineering strategies aiming at optimizing the production of ethanol and other bio-based chemicals. (via Semantic Scholar)
Sources: Crossref, NC State University Libraries, Web Of Science
Added: July 14, 2021

2021 journal article

Integrating Bioinformatics Tools into Inquiry-based Molecular Biology Laboratory Education Modules

Frontiers in Education, 6.

By: C. Goller n, M. Srougi n, S. Chen n, L. Schenkman n & R. Kelly n

author keywords: bioinformatics; science education; molecular biotechnology; software tools; case studies
TL;DR: This new paradigm for biology laboratory education that embraces bioinformatics as a critical component of inquiry-based learning for undergraduate and graduate students representing the life sciences, the physical sciences, and engineering is described. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: August 23, 2021

2021 journal article

Intersection of Biotic and Abiotic Sulfur Chemistry Supporting Extreme Microbial Life in Hot Acid

JOURNAL OF PHYSICAL CHEMISTRY B, 125(20), 5243–5257.

By: D. Willard n & R. Kelly n

MeSH headings : Metals; Oxidation-Reduction; Oxidoreductases / metabolism; Sulfolobus acidocaldarius; Sulfur
TL;DR: Extreme thermoacidophily, a characteristic of species in the archaeal order Sulfolobales, is considered from the perspective of sulfur chemistry, both biotic and abiotic, as it relates to microbial bioenergetics. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: June 14, 2021

2021 journal article

Intersection of Biotic and Abiotic Sulfur Chemistry Supporting Extreme Microbial Life in Hot Acid

Journal of Physical Chemistry B, 125(20), 5243–5257.

By: D. Willard & R. Kelly

Sources: Crossref, NC State University Libraries
Added: July 27, 2021

2021 journal article

The biology of thermoacidophilic archaea from the order Sulfolobales

FEMS Microbiology Reviews, 45(4).

By: A. Lewis n, A. Recalde*, C. Bräsen*, J. Counts n, P. Nussbaum*, J. Bost*, L. Schocke*, L. Shen* ...

author keywords: Archaea; Thermoacidophiles; Sulfolobales
MeSH headings : Archaea / genetics; Biology; Iron; Sulfolobales
TL;DR: The nuances of the thermoacidophilic lifestyle of the Sulfolobales are discussed, including their evolutionary placement, cell biology, survival strategies, genetic tools, metabolic processes, and physiological attributes together with how these characteristics make thermo Acidophiles ideal platforms for specialized industrial processes. (via Semantic Scholar)
Sources: Crossref, NC State University Libraries, Web Of Science
Added: July 14, 2021

2021 journal article

Thermophilic microbial deconstruction and conversion of natural and transgenic lignocellulose

Environmental Microbiology Reports, 13(3), 272–293.

By: R. Bing n, D. Sulis n, J. Wang n, M. Adams* & R. Kelly n

MeSH headings : Biomass; Lignin / chemistry; Plants / metabolism
TL;DR: Bioengineering plant properties to better fit the deconstruction capabilities of candidate consolidated bioprocessing microorganisms has potential to achieve the efficient lignocellulose deconstruction needed for industrial relevance. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Sources: Web Of Science, Crossref, NC State University Libraries, ORCID
Added: April 12, 2021

2021 journal article

Transcriptional Regulation of Plant Biomass Degradation and Carbohydrate Utilization Genes in the Extreme Thermophile Caldicellulosiruptor bescii

MSystems, 6(3).

By: D. Rodionov*, I. Rodionova*, V. Rodionov*, A. Arzamasov*, K. Zhang*, G. Rubinstein*, T. Tanwee*, R. Bing n ...

Ed(s): Z. Summers

TL;DR: The mechanisms of transcriptional regulation of carbohydrate utilization genes using a comparative genomics approach applied to 14 Caldicellulosiruptor species were investigated and it was confirmed that the shared ATPase component MsmK is essential for growth on oligo- and polysaccharides but not for the utilization of monosaccharides. (via Semantic Scholar)
Sources: Crossref, NC State University Libraries
Added: July 14, 2021

2020 journal article

Engineering the cellulolytic extreme thermophile Caldicellulosiruptor bescii to reduce carboxylic acids to alcohols using plant biomass as the energy source

Journal of Industrial Microbiology and Biotechnology, 47(8), 585–597.

By: G. Rubinstein*, G. Lipscomb*, A. Williams-Rhaesa*, G. Schut*, R. Kelly n & M. Adams*

author keywords: Thermophile; Biofuels; Lignocellulose; Metabolic engineering; Caldicellulosiruptor
MeSH headings : Alcohol Dehydrogenase / genetics; Alcohol Dehydrogenase / metabolism; Aldehyde Oxidoreductases / genetics; Aldehyde Oxidoreductases / metabolism; Biofuels / analysis; Biomass; Caldicellulosiruptor / genetics; Carboxylic Acids / metabolism; Ethanol / metabolism; Fermentation; Lignin / metabolism; Microorganisms, Genetically-Modified; Oxidation-Reduction; Panicum / metabolism; Panicum / microbiology; Pyrococcus furiosus / enzymology; Thermoanaerobacter / enzymology
TL;DR: This work investigates the ability of engineered C.bescii to generate alcohols from carboxylic acids and converts them to alcohol using both crystalline cellulose and switchgrass as sources of reductant for alcohol production, the first instance of an acid to alcohol conversion pathway in a cellulolytic microbe. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Sources: Web Of Science, NC State University Libraries, Crossref
Added: August 31, 2020

2020 journal article

Genome Sequences of Five Type Strain Members of the Archaeal Family Sulfolobaceae, Acidianus ambivalens, Acidianus infernus, Stygiolobus azoricus, Sulfuracidifex metallicus, and Sulfurisphaera ohwakuensis

MICROBIOLOGY RESOURCE ANNOUNCEMENTS, 9(11).

By: J. Counts n, N. Vitko n & R. Kelly n

TL;DR: Five genomes from the polyextremophilic (optimal temperature of >65°C and optimal pH of <3.5) archaeal family Sulfolobaceae are presented, greatly expanding order-wide genomic diversity. (via Semantic Scholar)
UN Sustainable Development Goal Categories
15. Life on Land (Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: April 6, 2020

2020 journal article

Life in hot acid: a genome‐based reassessment of the archaeal order Sulfolobales

Environmental Microbiology, 23(7), 3568–3584.

By: J. Counts n, D. Willard n & R. Kelly n

MeSH headings : Acidianus; Archaea / genetics; Hot Springs; Oxidation-Reduction; Phylogeny; Sulfolobaceae / genetics; Sulfolobales
TL;DR: Despite the presence of facultative anaerobes and one confirmed obligate anaerobe, oxidase complexes are prevalent in many species, suggesting a key role for oxygen among the Sulfolobales. (via Semantic Scholar)
UN Sustainable Development Goal Categories
15. Life on Land (Web of Science)
Sources: Web Of Science, Crossref, NC State University Libraries
Added: September 21, 2020

2020 journal article

Metabolically engineeredCaldicellulosiruptor besciias a platform for producing acetone and hydrogen from lignocellulose

BIOTECHNOLOGY AND BIOENGINEERING, 117(12), 3799–3808.

By: C. Straub n, R. Bing n, J. Otten n, L. Keller n, B. Zeldes n, M. Adams*, R. Kelly n

author keywords: acetone; Caldicellulosiruptor bescii; extreme thermophiles; metabolic engineering
MeSH headings : Acetone / metabolism; Biomass; Caldicellulosiruptor / genetics; Caldicellulosiruptor / metabolism; Hydrogen / metabolism; Lignin / metabolism; Metabolic Engineering
TL;DR: C. bescii can be engineered with balanced pathways in which renewable carbohydrate sources are converted to useful metabolites, primarily acetone and H2, without net production of its native fermentation products, acetate and lactate. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Sources: Web Of Science, NC State University Libraries
Added: September 7, 2020

2020 journal article

Modification of the glycolytic pathway in Pyrococcus furiosus and the implications for metabolic engineering

EXTREMOPHILES, 24(4), 511–518.

By: C. Straub n, G. Schut*, J. Otten n, L. Keller n, M. Adams* & R. Kelly n

author keywords: Pyrococcus; Thermophile; Central metabolism; Glycolysis
MeSH headings : Fermentation; Glyceraldehyde 3-Phosphate; Glycolysis; Metabolic Engineering; Pyrococcus furiosus
TL;DR: Insertion of a single gene encoding the thermostable NADPH-dependent primary alcohol dehydrogenase (adhA) from Caldanaerobacter subterraneus resulted in a strain producing ethanol via the previously established aldehyde oxidoreductase (AOR) pathway, and demonstrated a high ratio of ethanol over acetate at 80 °C and enabled ethanol production up to 85 °C, the highest temperature for bio-ethanol production reported to date. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Sources: Web Of Science, NC State University Libraries
Added: June 1, 2020

2020 article

The biology and biotechnology of the genus Caldicellulosiruptor: recent developments in 'Caldi World'

Lee, L. L., Crosby, J. R., Rubinstein, G. M., Laemthong, T., Bing, R. G., Straub, C. T., … Kelly, R. M. (2020, January). EXTREMOPHILES, Vol. 24, pp. 1–15.

By: L. Lee n, J. Crosby n, G. Rubinstein*, T. Laemthong n, R. Bing n, C. Straub n, M. Adams*, R. Kelly n

author keywords: Caldicellulosiruptor; Extreme thermophiles; Lignocellulose
MeSH headings : Biomass; Biotechnology; Clostridiales; Glycoside Hydrolases; Hot Springs
TL;DR: Improvements in the amount of carbohydrate released and in the production of bio-based chemicals are required to successfully realize the biotechnological potential of Caldicellulosiruptor organisms. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries, ORCID
Added: February 27, 2020

2020 journal article

Use of the lignocellulose-degrading bacterium Caldicellulosiruptor bescii to assess recalcitrance and conversion of wild-type and transgenic poplar

BIOTECHNOLOGY FOR BIOFUELS, 13(1).

By: C. Straub n, R. Bing n, J. Wang n, V. Chiang n, M. Adams* & R. Kelly n

author keywords: Caldicellulosiruptor; Extreme thermophiles; Lignocellulose; Biofuel; Poplar
TL;DR: Utilizing C. bescii as a screening assay for recalcitrance, poplar lines with down-regulation of coumarate 3-hydroxylase 3 (C3H3) resulted in the highest degrees of solubilization and conversion by C. Bescii, suggesting that excellent digestibility can be achieved without compromising fitness of the tree. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries, ORCID
Added: April 6, 2020

2019 journal article

Comparative Biochemical and Structural Analysis of Novel Cellulose Binding Proteins (Tapirins) from Extremely Thermophilic Caldicellulosiruptor Species

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 85(3).

By: L. Lee n, W. Hart n, V. Lunin, M. Alahuhta*, Y. Bomble*, M. Himmel*, S. Blumer-Schuette n, M. Adams*, R. Kelly n

author keywords: Caldicellulosiruptor; cellulose binding protein; lignocellulose; tapirin
MeSH headings : Amino Acid Sequence; Bacterial Proteins / chemistry; Bacterial Proteins / genetics; Bacterial Proteins / metabolism; Carrier Proteins / chemistry; Carrier Proteins / genetics; Carrier Proteins / metabolism; Cellulose / chemistry; Cellulose / metabolism; Firmicutes / chemistry; Firmicutes / genetics; Firmicutes / metabolism; Genome, Bacterial; Hot Springs / microbiology; Hot Temperature; Protein Domains
TL;DR: Densitometry analysis of bound protein fractions visualized by SDS-PAGE revealed that Calhy_0908 and Calkr_0826 (from weakly cellulolytic species) associated with the cellulose substrates to a greater extent than Athe_1870, Calkro_0844, and NA10_0869 (from strongly cellulolyic species). (via Semantic Scholar)
UN Sustainable Development Goal Categories
Sources: Web Of Science, NC State University Libraries
Added: February 11, 2019

2019 journal article

Determinants of sulphur chemolithoautotrophy in the extremely thermoacidophilic Sulfolobales

ENVIRONMENTAL MICROBIOLOGY, 21(10), 3696–3710.

By: B. Zeldes n, A. Loder n, J. Counts n, M. Haque n, K. Widney n, L. Keller n, S. Albers*, R. Kelly n

MeSH headings : Autotrophic Processes; Chemoautotrophic Growth; Oxidation-Reduction; Oxidoreductases / metabolism; Sulfolobales / metabolism; Sulfur / metabolism; Thiosulfates / metabolism
TL;DR: Comparative transcriptomic analyses of Acidianus brierleyi, a chemolithoautotrophic sulfur oxidizer, and S. acidocaldarius DSM639 showed that while both share a strong transcriptional response to elemental sulfur, both failed to up-regulate key 3-HP/4-HB cycle genes used by A. briersleyi to drive chemolithOautotrophy. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: August 12, 2019

2019 journal article

Extreme thermophiles as emerging metabolic engineering platforms

Current Opinion in Biotechnology, 59, 55–64.

By: J. Crosby n, T. Laemthong n, A. Lewis n, C. Straub n, M. Adams* & R. Kelly n

MeSH headings : Archaea; Biotechnology; Genetic Engineering; Metabolic Engineering; Waste Products
TL;DR: If non-model platforms are successfully deployed, new avenues for production of fuels and chemicals from renewable feedstocks or waste materials will emerge. (via Semantic Scholar)
Sources: Web Of Science, Crossref, NC State University Libraries
Added: November 25, 2019

2019 journal article

Extremely Thermoacidophilic Metallosphaera Species Mediate Mobilization and Oxidation of Vanadium and Molybdenum Oxides

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 85(5).

By: G. Wheaton n, N. Vitko n, J. Counts n, J. Dulkis n, I. Podolsky n, A. Mukherjee n, R. Kelly n

author keywords: Metallosphaera; biomining; extreme thermoacidophily; metal biooxidation; molybdenum; vanadium
MeSH headings : Archaeal Proteins / genetics; Copper / metabolism; Ferric Compounds / metabolism; Gene Expression Profiling; Genome, Archaeal; Hot Temperature; Hydrogen-Ion Concentration; Molybdenum / metabolism; Oxidation-Reduction; Oxides / metabolism; Oxygen; Sulfolobaceae / genetics; Sulfolobaceae / metabolism; Sulfur Compounds / metabolism; Transcriptome; Uranium / metabolism; Vanadium / metabolism
TL;DR: Results suggest that direct oxidation of V and Mo occurs, in addition to Fe-mediated oxidation, such that both direct and indirect mechanisms are involved in the mobilization of redox-active metals by Metallosphaera species. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: March 11, 2019

2019 journal article

Lignocellulose solubilization and conversion by extremely thermophilic Caldicellulosiruptor bescii improves by maintaining metabolic activity

BIOTECHNOLOGY AND BIOENGINEERING, 116(8), 1901–1908.

By: C. Straub n, P. Khatibi n, J. Otten n, M. Adams* & R. Kelly n

author keywords: biofuels; bioreactor; Caldicellulosiruptor bescii; lignocellulose; switchgrass
MeSH headings : Biofuels / microbiology; Bioreactors; Caldicellulosiruptor; Fermentation; Firmicutes / growth & development; Firmicutes / metabolism; Lignin / metabolism; Panicum / metabolism; Solubility
TL;DR: The extreme thermophile Caldicellulosiruptor bescii solubilized carbohydrate conversion to fermentation products increased from 40% in batch to over 80% in the purged system, an improvement attributed to maintaining the bioreactor culture in a metabolically active state. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Sources: Web Of Science, NC State University Libraries
Added: August 19, 2019

2019 journal article

Quantitative fermentation of unpretreated transgenic poplar by Caldicellulosiruptor bescii

NATURE COMMUNICATIONS, 10(1).

By: C. Straub n, P. Khatibi n, J. Wang n, J. Conway n, A. Williams-Rhaesa*, I. Peszlen n, V. Chiang n, M. Adams*, R. Kelly n

Contributors: C. Straub n, P. Khatibi n, J. Wang n, J. Conway n, A. Williams-Rhaesa*, I. Peszlen n, V. Chiang n, M. Adams*, R. Kelly n

MeSH headings : Biomass; Cellulose / metabolism; Clostridiales / genetics; Clostridiales / metabolism; Ethanol / metabolism; Fermentation; Industrial Microbiology; Lignin / metabolism; Metabolic Engineering; Plants, Genetically Modified / chemistry; Plants, Genetically Modified / genetics; Plants, Genetically Modified / metabolism; Polysaccharides / metabolism; Populus / chemistry; Populus / genetics; Populus / metabolism
TL;DR: The authors pair transgenic lignin modified poplar lines with engineered Caldicellusiruptor bescii to achieve biomass solubilization and ethanol conversion without pretreatment and the nearly quantitative microbial conversion of the carbohydrate content of unpretreated transgen lignocellulosic biomass bodes well for full utilization of renewable biomass feedstocks. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries, ORCID
Added: August 26, 2019

2019 journal article

The thermophilic biomass-degrading bacterium Caldicellulosiruptor bescii utilizes two enzymes to oxidize glyceraldehyde 3-phosphate during glycolysis

JOURNAL OF BIOLOGICAL CHEMISTRY, 294(25), 9995–10005.

By: I. Scott*, G. Rubinstein*, F. Poole*, G. Lipscomb*, G. Schut*, A. Williams-Rhaesa*, D. Stevenson*, D. Amador-Noguez*, R. Kelly n, M. Adams*

author keywords: glycolysis; cellulose; metabolism; metabolomics; metalloenzyme; oxidation-reduction (redox); anaerobic glycolysis; iron-sulfur protein; pterin; tungsten
MeSH headings : Biomass; Caldicellulosiruptor; Firmicutes / growth & development; Firmicutes / metabolism; Glyceraldehyde 3-Phosphate / chemistry; Glyceraldehyde 3-Phosphate / metabolism; Glyceraldehyde 3-Phosphate Dehydrogenase (NADP+) / metabolism; Glyceraldehyde-3-Phosphate Dehydrogenases / metabolism; Glycolysis; Metabolome; Oxidation-Reduction; Phylogeny
TL;DR: Using metabolomics and kinetic analyses, it is shown that GOR functions in parallel with the conventional GAP dehydrogenase, providing an alternative ferredoxin-dependent glycolytic pathway and likely facilitates the recycling of reduced redox carriers (NADH and ferredox) in response to environmental H2 concentrations. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Sources: Web Of Science, NC State University Libraries
Added: July 22, 2019

2018 journal article

A synthetic enzymatic pathway for extremely thermophilic acetone production based on the unexpectedly thermostable acetoacetate decarboxylase from Clostridium acetobutylicum

BIOTECHNOLOGY AND BIOENGINEERING, 115(12), 2951–2961.

By: B. Zeldes n, C. Straub n, J. Otten n, M. Adams* & R. Kelly n

author keywords: acetone; biotransformations; extreme thermophiles
MeSH headings : Acetone / metabolism; Bacterial Proteins / genetics; Bacterial Proteins / metabolism; Carboxy-Lyases / genetics; Carboxy-Lyases / metabolism; Clostridium acetobutylicum / enzymology; Clostridium acetobutylicum / genetics; Clostridium acetobutylicum / metabolism; Enzyme Stability; Hot Temperature; Metabolic Engineering; Metabolic Networks and Pathways / genetics; Synthetic Biology / methods
TL;DR: A synthetic enzymatic pathway for acetone production that functions up to at least 70°C in vitro, made possible by the unusual thermostability of Adc from the mesophile C. acetobutylicum, paving the way for bio‐reactive distillation of acetone using a metabolically engineered extreme thermophile as a production host. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Sources: Web Of Science, NC State University Libraries
Added: December 3, 2018

2018 review

Biotechnology of extremely thermophilic archaea

[Review of ]. FEMS MICROBIOLOGY REVIEWS, 42(5), 543–578.

By: C. Straub n, J. Counts n, D. Nguyen*, C. Wu*, B. Zeldes n, J. Crosby n, J. Conway n, J. Otten n ...

author keywords: extremely thermophilic archaea; biotechnology; biocatalysis; biooxidation
MeSH headings : Archaea / genetics; Archaea / physiology; Biotechnology / trends; Hot Temperature; Industrial Microbiology / trends; Metabolic Engineering / trends
TL;DR: There are barriers and challenges to these organisms reaching their full potential as industrial microorganisms but, if these can be overcome, a new dimension for biotechnology will be forthcoming that strategically exploits biology at high temperatures. (via Semantic Scholar)
UN Sustainable Development Goal Categories
15. Life on Land (Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: November 19, 2018

2018 journal article

Complete Genome Sequences of Extremely Thermoacidophilic Metal-Mobilizing Type Strain Members of the Archaeal Family Sulfolobaceae, Acidianus brierleyi DSM-1651, Acidianus sulfidivorans DSM-18786, and Metallosphaera hakonensis DSM-7519

MICROBIOLOGY RESOURCE ANNOUNCEMENTS, 7(2).

By: J. Counts n, N. Vitko n & R. Kelly n

TL;DR: Three closed genomes from two currently defined genera within the family Sulfolobaceae are reported, namely, Acidianus brierleyi DSM-1651T,Acidianus sulfidivorans DSM-18786T, and Metallosphaera hakonensis DSM-7519T. (via Semantic Scholar)
UN Sustainable Development Goal Categories
15. Life on Land (Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: December 31, 2018

2018 journal article

Engineering redox-balanced ethanol production in the cellulolytic and extremely thermophilic bacterium, Caldicellulosiruptor bescii

Metabolic Engineering Communications, 7, e00073.

By: A. Williams-Rhaesa*, G. Rubinstein*, I. Scott*, G. Lipscomb*, F. Poole, II, R. Kelly n, M. Adams*

TL;DR: A strain of C. bescii was engineered using a more stable genetic background than previously reported and heterologously-expressed AdhE resulted in increased ethanol production compared to previous reports, which underlines the importance of redox balancing and paves the way for achieving even higher ethanol titers in C. Bescii. (via Semantic Scholar)
UN Sustainable Development Goal Categories
7. Affordable and Clean Energy (OpenAlex)
Sources: Crossref, NC State University Libraries
Added: February 24, 2020

2018 journal article

Genus-Wide Assessment of Lignocellulose Utilization in the Extremely Thermophilic Genus Caldicellulosiruptor by Genomic, Pangenomic, and Metagenomic Analyses

Applied and Environmental Microbiology, 84(9), e02694–17.

By: L. Lee n, S. Blumer-Schuette n, J. Izquierdo n, J. Zurawski n, A. Loder n, J. Conway n, J. Elkins*, M. Podar* ...

Ed(s): I. Cann

author keywords: Caldicellulosiruptor; extreme thermophile; pangenome
MeSH headings : Cellulose / metabolism; Firmicutes / classification; Firmicutes / genetics; Firmicutes / metabolism; Genome, Bacterial; Genomics; Lignin / metabolism; Metagenome; Metagenomics
TL;DR: It is indicated that microcrystalline cellulose degradation at temperatures above 70°C, based on current information, is limited to certain members of this genus that produce GH48 domain-containing enzymes. (via Semantic Scholar)
UN Sustainable Development Goal Categories
15. Life on Land (OpenAlex)
Sources: Crossref, NC State University Libraries
Added: July 27, 2021

2018 journal article

Native xylose-inducible promoter expands the genetic tools for the biomass-degrading, extremely thermophilic bacterium Caldicellulosiruptor bescii

EXTREMOPHILES, 22(4), 629–638.

By: A. Williams-Rhaesa*, N. Awuku*, G. Lipscomb*, F. Poole*, G. Rubinstein*, J. Conway n, R. Kelly n, M. Adams*

author keywords: Anaerobes; Biodegradation of cellulosic; Biotechnology of thermophiles; Genetics; Molecular biology; Genetics of extremophiles
MeSH headings : Biotransformation; Cellulose / metabolism; Firmicutes / drug effects; Firmicutes / genetics; Firmicutes / growth & development; Firmicutes / metabolism; Industrial Microbiology / methods; L-Lactate Dehydrogenase / genetics; L-Lactate Dehydrogenase / metabolism; Promoter Regions, Genetic; Thermotolerance; Xylose / metabolism; Xylose / pharmacology
TL;DR: This present study expands the tools available for genetically engineering Caldicellulosiruptor bescii, the most thermophilic cellulose-degrader known growing up to 90 °C on unpretreated plant biomass, by utilizing a native xylose-inducible promoter to control the expression of the reporter gene encoding lactate dehydrogenase. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2018 journal article

Novel multidomain, multifunctional glycoside hydrolases from highly lignocellulolytic Caldicellulosiruptor species

AICHE JOURNAL, 64(12), 4218–4228.

By: J. Conway n, J. Crosby n, A. Hren n, R. Southerland n, L. Lee n, V. Lunin, P. Alahuhta*, M. Himmel* ...

author keywords: Caldicellulosiruptor; lignocellulose; glycoside hydrolase; cellulase
UN Sustainable Development Goal Categories
Sources: Web Of Science, NC State University Libraries
Added: November 26, 2018

2018 journal article

Parsing in vivo and in vitro contributions to microcrystalline cellulose hydrolysis by multidomain glycoside hydrolases in the Caldicellulosiruptor bescii secretome

BIOTECHNOLOGY AND BIOENGINEERING, 115(10), 2426–2440.

By: J. Conway n, J. Crosby n, B. McKinley n, N. Seals n, M. Adams* & R. Kelly n

author keywords: Caldicellulosiruptor berci; cellulase; extreme thermophile; glycoside hydrolase; lignocellulose; secretome
MeSH headings : Cellulose / chemistry; Cellulose / metabolism; Firmicutes / enzymology; Firmicutes / genetics; Glycoside Hydrolases / chemistry; Glycoside Hydrolases / genetics; Glycoside Hydrolases / metabolism; Hydrolysis
TL;DR: Results suggest that the C. bescii secretome is naturally balanced to achieve optimal synergy for cellulose degradation and reinforce the importance of microbial contributions to microcrystalline cellulose hydrolysis and suggest that mass action effects from glucan fermentation shift equilibria to drive degradation. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Sources: Web Of Science, NC State University Libraries
Added: October 29, 2018

2018 journal article

Secretion and fusion of biogeochemically active archaeal membrane vesicles

GEOBIOLOGY, 16(6), 659–673.

By: T. Johnson*, C. Mach*, R. Grove*, R. Kelly n, K. Van Cott* & P. Blum*

author keywords: archaea; membrane fusion; regulated secretion; thermoacidophiles; vesicles
MeSH headings : Archaea / metabolism; Archaea / physiology; Archaeal Proteins / metabolism
TL;DR: Membrane vesicles may play a role in modulating energy‐related traits in geochemical environments by fusion‐mediated protein delivery as well as in response to unknown signals and for undefined purposes. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Sources: Web Of Science, NC State University Libraries
Added: October 29, 2018

2018 journal article

Sequential processing with fermentative Caldicellulosiruptor kronotskyensis and chemolithoautotrophic Cupriavidus necator for converting rice straw and CO2 to polyhydroxybutyrate

BIOTECHNOLOGY AND BIOENGINEERING, 115(6), 1624–1629.

By: X. Peng*, R. Kelly n & Y. Han*

author keywords: biorefinery; Caldicellulosiruptor; CO2 fixation; Cupriavidus necator; fermentation; lignocellulose
MeSH headings : Animals; Carbon Dioxide / metabolism; Cupriavidus necator / growth & development; Cupriavidus necator / metabolism; Firmicutes / growth & development; Firmicutes / metabolism; Hydroxybutyrates / metabolism; Oryza / metabolism; Plant Stems / metabolism; Polyesters / metabolism
TL;DR: The sequential coupling of C. kronotskyensis and C. necator provides not only a new biorefinery paradigm characterized by reduced pretreatment and saccharification requirements but also an efficient way for enhancing CO2 fixation. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2018 journal article

Simultaneous biosynthesis of (R)-acetoin and ethylene glycol from D-xylose through in vitro metabolic engineering

Metabolic Engineering Communications, 7, e00074.

By: X. Jia*, R. Kelly n & Y. Han*

TL;DR: It is shown here that a cell-free bioreaction scheme can generate enantiomerically pure acetoin and EG as co-products from biomass-derived D-xylose, illustrating the potential for production of multiple value-added biomolecules from biomass -based sugars with no ATP requirement. (via Semantic Scholar)
Sources: Crossref, NC State University Libraries
Added: February 24, 2020

2018 journal article

The diversity and specificity of the extracellular proteome in the cellulolytic bacterium Caldicellulosiruptor bescii is driven by the nature of the cellulosic growth substrate

BIOTECHNOLOGY FOR BIOFUELS, 11.

By: S. Poudel*, R. Giannone*, M. Basen*, I. Nookaew*, F. Poole*, R. Kelly*, M. Adams*, R. Hettich*

author keywords: Caldicellulosiruptor bescii; Carbohydrate-active enzymes (CAZymes); Extracellular solute binding proteins (ESBPs); Protein of unknown function (PUF); Glycosyl hydrolases (GH); Mass spectrometry; Switchgrass; Xylan; Avicel; C5 substrates; C6 substrates; Lignocellulosic; Extracellular
TL;DR: This study reveals the protein membership of the C. bescii secretome and demonstrates its plasticity based on the complexity (mono-/disaccharides vs. polysaccharides) and type of carbon (C5 vs. C6) available to the microorganism. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2018 journal article

Uncoupling Fermentative Synthesis of Molecular Hydrogen from Biomass Formation in Thermotoga maritima

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 84(17).

By: R. Singh*, D. White*, Y. Demirel*, R. Kelly n, K. Noll* & P. Blum*

author keywords: Hyperthermophile; MalK; hydrogen; transient gene inactivation; adaptive laboratory evolution; bacterial genetics; fermentation; hydrogen; Thermotoga maritima
MeSH headings : ATP-Binding Cassette Transporters / genetics; Bacterial Proteins / genetics; Biological Transport / genetics; Biomass; Bioreactors / microbiology; Energy Metabolism / genetics; Energy Metabolism / physiology; Hydrogen / metabolism; L-Lactate Dehydrogenase / genetics; Lactic Acid / biosynthesis; Maltose / metabolism; Thermotoga maritima / genetics; Thermotoga maritima / metabolism
TL;DR: Cell lines of the extremophile Thermotoga maritima are described that exceed the physiologic limits for H2 formation arising from genetic changes in fermentative metabolism, indicating that a reduced rate of maltose uptake was accompanied by an increase in H2 production, changing fermentation efficiency and shifting energy management. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Sources: Web Of Science, NC State University Libraries
Added: October 19, 2018

2017 journal article

Bioavailability of Carbohydrate Content in Natural and Transgenic Switchgrasses for the Extreme Thermophile Caldicellulosiruptor bescii

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 83(17).

By: J. Zurawski n, P. Khatibi n, H. Akinosho*, C. Straub n, S. Compton n, J. Conway n, L. Lee n, A. Ragauskas* ...

author keywords: Caldicellulosiruptor; switchgrass; lignocellulose deconstruction and conversion; extreme thermophiles; lignocellulose
MeSH headings : Biomass; Fermentation; Gram-Positive Bacteria / genetics; Gram-Positive Bacteria / metabolism; Hot Temperature; Lignin / chemistry; Lignin / metabolism; Panicum / chemistry; Panicum / genetics; Panicum / metabolism; Panicum / microbiology; Plants, Genetically Modified / chemistry; Plants, Genetically Modified / genetics; Plants, Genetically Modified / metabolism; Plants, Genetically Modified / microbiology; Polysaccharides / chemistry; Polysaccharides / metabolism
TL;DR: While C. bescii could significantly solubilize the transgenic switchgrass lines and natural variant tested here, additional or alternative strategies (physical, chemical, enzymatic, and/or genetic) are needed to eliminate recalcitrance. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2017 journal article

C aldicellulosiruptor saccharolyticus transcriptomes reveal consequences of chemical pretreatment and genetic modification of lignocellulose

Microbial Biotechnology, 10(6), 1546–1557.

By: S. Blumer‐Schuette n, J. Zurawski n, J. Conway n, P. Khatibi n, D. Lewis n, Q. Li n, V. Chiang n, R. Kelly n

MeSH headings : Acids / chemistry; Bacterial Proteins / genetics; Bacterial Proteins / metabolism; Biotransformation; Firmicutes / genetics; Firmicutes / metabolism; Lignin / metabolism; Populus / chemistry; Populus / genetics; Populus / metabolism; Populus / microbiology; Transcriptome
TL;DR: The results here present a new paradigm for assessing plant–microbe interactions that can be deployed as a biological assay to report on the complexity and recalcitrance of plant biomass. (via Semantic Scholar)
Sources: Crossref, NC State University Libraries
Added: July 21, 2021

2017 journal article

Diversity of bacteria and archaea from two shallow marine hydrothermal vents from Vulcano Island

Extremophiles, 21(4), 733–742.

By: G. Antranikian*, M. Suleiman*, C. Schäfers*, M. Adams, S. Bartolucci, J. Blamey, N. Birkeland, E. Bonch-Osmolovskaya ...

author keywords: Hyperthermophiles; Archaea; Diversity; Hydrothermal marine shallow vents
MeSH headings : Archaea / classification; Archaea / genetics; Bacteria / classification; Bacteria / genetics; Hydrothermal Vents / microbiology; Italy; Marine Biology; RNA, Ribosomal, 16S / genetics
TL;DR: Sediment and water samples taken from two shallow marine hydrothermal vents with temperatures of 100 °C at Vulcano Island, Italy showed a high abundance of hyperthermophilic archaea and a comparable diverse archaeal community composition in both samples, and the microbial diversity of the enrichment cultures changed significantly. (via Semantic Scholar)
UN Sustainable Development Goal Categories
14. Life Below Water (OpenAlex)
Sources: Crossref, NC State University Libraries
Added: August 3, 2021

2017 journal article

Ethanol production by the hyperthermophilic archaeon Pyrococcus furiosus by expression of bacterial bifunctional alcohol dehydrogenases

Microbial Biotechnology, 10(6), 1535–1545.

By: M. Keller*, G. Lipscomb*, D. Nguyen*, A. Crowley*, G. Schut*, I. Scott*, R. Kelly n, M. Adams*

TL;DR: The AOR gene was deleted from P. furiosus to evaluate ethanol production directly from acetyl‐CoA by heterologous expression of the adhE gene from eight thermophilic bacteria, and the highest amount of ethanol was produced whenadhE and adhA from Thermoanaerobacter were co‐expressed. (via Semantic Scholar)
Sources: Crossref, NC State University Libraries
Added: July 21, 2021

2017 review

Extremely thermophilic energy metabolisms: biotechnological prospects

[Review of ]. CURRENT OPINION IN BIOTECHNOLOGY, 45, 104–112.

By: C. Straub n, B. Zeldes n, G. Schut*, M. Adams* & R. Kelly n

MeSH headings : Archaea / classification; Archaea / genetics; Archaea / metabolism; Biotechnology / methods; Carbon Dioxide / metabolism; Chemoautotrophic Growth; Energy Metabolism; Hot Temperature; Lignin / metabolism; Metabolic Engineering
TL;DR: This review considers recent developments in extreme thermophile biology as they relate to new horizons for energy biotechnology. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2017 journal article

Functional Analysis of the Glucan Degradation Locus in Caldicellulosiruptor bescii Reveals Essential Roles of Component Glycoside Hydrolases in Plant Biomass Deconstruction

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 83(24).

By: J. Conway n, B. McKinley n, N. Seals n, D. Hernandez n, P. Khatibi n, S. Poudel*, R. Giannone*, R. Hettich* ...

author keywords: Caldicellulosiruptor; extreme thermophile; cellulose degradation; lignocellulose; glycoside hydrolase; cellulase
MeSH headings : Bacterial Proteins / metabolism; Cellulose / chemistry; Firmicutes / genetics; Firmicutes / metabolism; Glucans / metabolism; Glycoside Hydrolases / metabolism; Panicum / chemistry; Populus / chemistry
TL;DR: It is demonstrated that certain GDL GHs are primarily responsible for the degradation of microcrystalline cellulose-containing substrates by C. bescii and provide new insights into the workings of a novel microbial mechanism for lignocellulose utilization. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2017 journal article

Genome Stability in Engineered Strains of the Extremely Thermophilic Lignocellulose-Degrading Bacterium Caldicellulosiruptor bescii

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 83(14).

By: A. Williams-Rhaesa*, F. Poole*, J. Dinsmore*, G. Lipscomb*, G. Rubinstein*, I. Scott*, J. Conway n, L. Lee n ...

author keywords: Southern blotting; genome sequencing; insertion elements; kanamycin resistance
MeSH headings : Bacterial Proteins / genetics; Bacterial Proteins / metabolism; Genetic Engineering; Genome, Bacterial; Genomic Instability; Gram-Positive Bacteria / genetics; Gram-Positive Bacteria / metabolism; Hot Temperature; Lignin / metabolism
TL;DR: It is shown that strains derived from the genetic background containing a random deletion in uracil biosynthesis genes (pyrFA) have a dramatic increase in the number of single nucleotide polymorphisms, insertions/deletions, and ISCbe4 insertion elements in their genomes compared to the wild type. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2017 journal article

Impact of growth mode, phase, and rate on the metabolic state of the extremely thermophilic archaeon Pyrococcus furiosus

BIOTECHNOLOGY AND BIOENGINEERING, 114(12), 2947–2954.

By: P. Khatibi n, C. Chou n, A. Loder n, J. Zurawski n, M. Adams* & R. Kelly n

author keywords: continuous culture; growth phase; growth rate; hyperthermophiles; Pyrococcus furiosus; transcriptome
MeSH headings : Archaeal Proteins / metabolism; Batch Cell Culture Techniques / methods; Cell Proliferation / physiology; Energy Metabolism / physiology; Pyrococcus furiosus / growth & development; Pyrococcus furiosus / metabolism; Transcriptome / physiology
TL;DR: The results here suggest that cellular processes involved in carbon and electron flux in P. furiosus were significantly impacted by growth mode, phase and rate, factors that need to be taken into account when developing successful metabolic engineering strategies. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2017 review

Physiological, metabolic and biotechnological features of extremely thermophilic microorganisms

[Review of ]. WILEY INTERDISCIPLINARY REVIEWS-SYSTEMS BIOLOGY AND MEDICINE, 9(3).

By: J. Counts n, B. Zeldes n, L. Lee n, C. Straub n, M. Adams* & R. Kelly n

MeSH headings : Biocatalysis; Carbohydrate Metabolism; Carbon Dioxide / metabolism; Glycolysis; Metabolic Engineering; Metals / chemistry; Metals / metabolism; Sulfolobales / metabolism; Sulfur / metabolism; Thermoanaerobacter / metabolism; Thermococcales / metabolism; Thermus / metabolism
TL;DR: The bacterial genera Caldicellulosiruptor, Thermotoga and Thermus, and the archaea belonging to the orders Thermococcales and Sulfolobales, are among the most studied extreme thermophiles to date. (via Semantic Scholar)
UN Sustainable Development Goal Categories
14. Life Below Water (OpenAlex)
15. Life on Land (Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2017 report

Sequestration of CO2 with H2 into useful products

(US Patent No. 9,587,256).

By: M. Adams, R. Kelly, A. Hawkins, A. Menon, G. Lipscomb & G. Schut

Source: NC State University Libraries
Added: August 27, 2021

2017 article

The renaissance of life near the boiling point - at last, genetics and metabolic engineering

Adams, M. W. W., & Kelly, R. M. (2017, January). MICROBIAL BIOTECHNOLOGY, Vol. 10, pp. 37–39.

By: M. Adams* & R. Kelly n

MeSH headings : Biotechnology / methods; Hot Temperature; Industrial Microbiology / methods; Metabolic Engineering / methods
TL;DR: The prospects for biotechnology of extreme thermophilic microorganisms are discussed here and the implications are discussed for science, medicine, and society. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2017 journal article

Two Distinct alpha-L-Arabinofuranosidases in Caldicellulosiruptor Species Drive Degradation of Arabinose-Based Polysaccharides

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 83(13).

By: M. Abu Saleh, W. Han*, M. Lu*, B. Wang*, H. Li*, R. Kelly n, F. Li*

author keywords: Arabinofuranosidase; bioenergy; glycoside hydrolase; hyperthermophiles; synergism
MeSH headings : Arabinose / metabolism; Bacterial Proteins / chemistry; Bacterial Proteins / genetics; Bacterial Proteins / metabolism; Firmicutes / chemistry; Firmicutes / enzymology; Firmicutes / genetics; Firmicutes / metabolism; Glycoside Hydrolases / chemistry; Glycoside Hydrolases / genetics; Glycoside Hydrolases / metabolism; Hydrogen-Ion Concentration; Molecular Weight; Polysaccharides / metabolism; Substrate Specificity
TL;DR: The data suggest that plant biomass degradation in Caldicellulosiruptor species involves extracellular XynF that acts synergistically with other hemicellULases to digest arabino-polysaccharides that are subsequently transported and degraded further by intracellular AbF51 to produce short-chain arabinos sugars. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2017 journal article

VapC toxins drive cellular dormancy under uranium stress for the extreme thermoacidophile Metallosphaera prunae

ENVIRONMENTAL MICROBIOLOGY, 19(7), 2831–2842.

By: A. Mukherjee n, G. Wheaton n, J. Counts n, B. Ijeomah n, J. Desai n & R. Kelly n

MeSH headings : Archaeal Proteins / genetics; Archaeal Proteins / metabolism; Bacterial Toxins / genetics; Bacterial Toxins / metabolism; Gene Expression Regulation, Bacterial / physiology; RNA Stability / physiology; Sulfolobaceae / genetics; Sulfolobaceae / growth & development; Sulfolobaceae / metabolism; Transcriptome; Uranium / metabolism
TL;DR: M. prunae utilizes VapC toxins for post-transcriptional regulation under uranium stress to enter a cellular dormant state, thereby providing an adaptive response to what would otherwise be a deleterious environmental perturbation. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2016 journal article

A Highly Thermostable Kanamycin Resistance Marker Expands the Tool Kit for Genetic Manipulation of Caldicellulosiruptor bescii

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 82(14), 4421–4428.

By: G. Lipscomb*, J. Conway n, S. Blumer-Schuette n, R. Kelly n & M. Adams*

MeSH headings : Firmicutes / genetics; Firmicutes / radiation effects; Genetics, Microbial / methods; Genomic Instability / radiation effects; Hot Temperature; Kanamycin Resistance; Molecular Biology / methods; Selection, Genetic
TL;DR: A selection system for C. bescii using the antibiotic kanamycin is developed and it is shown that, in combination with the biosynthetic gene marker, it can be used to efficiently delete genes in this organism. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2016 journal article

Ancillary contributions of heterologous biotin protein ligase and carbonic anhydrase for CO2 incorporation into 3-hydroxypropionate by metabolically engineered Pyrococcus furiosus

Biotechnology and Bioengineering, 113(12), 2652–2660.

By: H. Lian n, B. Zeldes n, G. Lipscomb*, A. Hawkins n, Y. Han n, A. Loder n, D. Nishiyama n, M. Adams*, R. Kelly n

author keywords: biotin protein ligase; carboxylase; carbon dioxide; Pyrococcus furiosus; 3-hydroxypropionate; Metallosphaera sedula
MeSH headings : Carbon Dioxide / chemistry; Carbon Dioxide / metabolism; Carbon-Nitrogen Ligases / metabolism; Carbonic Anhydrases / genetics; Escherichia coli Proteins / metabolism; Lactic Acid / analogs & derivatives; Lactic Acid / biosynthesis; Lactic Acid / chemistry; Metabolic Engineering / methods; Protein Engineering / methods; Pyrococcus furiosus / physiology; Recombinant Proteins / genetics; Recombinant Proteins / metabolism; Repressor Proteins / metabolism; Sulfolobaceae / genetics; Sulfolobaceae / metabolism
TL;DR: The results illustrate the ancillary benefits of accessory enzymes for incorporating CO2 into 3HP production in metabolically engineered P. furiosus, and hint at the important role that CA and BPL likely play in the native 3HP/4HB pathway in M. sedula. (via Semantic Scholar)
UN Sustainable Development Goal Categories
6. Clean Water and Sanitation (OpenAlex)
Sources: Crossref, NC State University Libraries
Added: December 31, 2020

2016 chapter

Extreme Thermophiles as Metabolic Engineering Platforms: Strategies and Current Perspective

In C. Wittmann & J. C. Liao (Eds.), Industrial Biotechnology: Microorganisma (Vol. 1, pp. 505–580).

By: A. Loder n, B. Zeldes n, J. Conway n, J. Counts n, C. Straub n, P. Khatibi n, L. Lee n, N. Vitko n ...

Ed(s): C. Wittmann & J. Liao

Sources: Crossref, NC State University Libraries
Added: July 30, 2021

2016 journal article

Heterologous Production of an Energy-Conserving Carbon Monoxide Dehydrogenase Complex in the Hyperthermophile Pyrococcus furiosus

FRONTIERS IN MICROBIOLOGY, 7.

By: G. Schut*, G. Lipscomb*, D. Nguyen*, R. Kelly n & M. Adams*

author keywords: hyperthermophile; archaea; hydrogen; carbon monoxide; energy; anaerobic respiration; Thermococcales
TL;DR: The functional transfer of CO utilization between Thermococcus and Pyrococcus species demonstrated herein is representative of the horizontal gene transfer of an environmentally relevant metabolic capability. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2016 journal article

Multidomain, Surface Layer-associated Glycoside Hydrolases Contribute to Plant Polysaccharide Degradation by Caldicellulosiruptor Species

JOURNAL OF BIOLOGICAL CHEMISTRY, 291(13), 6732–6747.

By: J. Conway n, W. Pierce n, J. Le n, G. Harper n, J. Wright n, A. Tucker n, J. Zurawski n, L. Lee n, S. Blumer-Schuette n, R. Kelly n

author keywords: biofuel; cell surface; cell surface enzyme; enzyme; glycoside hydrolase; plant cell wall; Caldicellulosiruptor; S-layer; lignocellulose
MeSH headings : Bacterial Proteins / chemistry; Bacterial Proteins / genetics; Bacterial Proteins / metabolism; Cloning, Molecular; Clostridiales / chemistry; Clostridiales / classification; Clostridiales / enzymology; Escherichia coli / genetics; Escherichia coli / metabolism; Gene Expression; Genome, Bacterial; Glucans / metabolism; Glycoside Hydrolases / chemistry; Glycoside Hydrolases / genetics; Glycoside Hydrolases / metabolism; Kinetics; Mutation; Phylogeny; Polysaccharides / metabolism; Protein Binding; Protein Engineering; Protein Structure, Tertiary; Recombinant Proteins / chemistry; Recombinant Proteins / genetics; Recombinant Proteins / metabolism; Substrate Specificity; Wood / metabolism; Xylans / metabolism
TL;DR: The results here provide new insights into the architecture and role of SLHdomain GHs and demonstrate that hemicellulose degradation can be enhanced through non-native SLH domain GHs engineered into the genomes of Caldicelluosiruptor species. (via Semantic Scholar)
UN Sustainable Development Goal Categories
15. Life on Land (OpenAlex)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2016 journal article

Reaction kinetic analysis of the 3-hydroxypropionate/4-hydroxybutyrate CO2 fixation cycle in extremely thermoacidophilic archaea

METABOLIC ENGINEERING, 38, 446–463.

By: A. Loder n, Y. Han n, A. Hawkins n, H. Lian n, G. Lipscomb*, G. Schut*, M. Keller*, M. Adams*, R. Kelly n

author keywords: CO2 fixation; 3-hydroxypropionate; 4-hydroxybutyrate; Metallosphaera sedula
MeSH headings : Archaea / metabolism; Carbon Dioxide / metabolism; Extremophiles / metabolism; Hydroxybutyrates / metabolism; Kinetics; Lactic Acid / analogs & derivatives; Lactic Acid / metabolism; Metabolic Clearance Rate; Metabolic Flux Analysis / methods; Metabolic Networks and Pathways / physiology; Models, Biological; Signal Transduction / physiology; Sulfolobaceae / metabolism
TL;DR: A reaction kinetics model was developed to examine the biological and biotechnological attributes of the 3HP/4HB cycle as it operates in Metallosphaera sedula and assess metabolic engineering strategies for incorporating CO2 into chemical intermediates and products of bioteschnological importance: acetyl-CoA, succinate, and 3-hydroxypropionate. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2016 journal article

Temperature-dependent acetoin production by Pyrococcus furiosus is catalyzed by a biosynthetic acetolactate synthase and its deletion improves ethanol production

METABOLIC ENGINEERING, 34, 71–79.

By: D. Nguyen*, G. Lipscomb*, G. Schut*, B. Vaccaro*, M. Basen*, R. Kelly n, M. Adams*

author keywords: Archaea; Hyperthermophile; Genetic engineering; Acetolactate synthase; Acetoin; Ethanol
MeSH headings : Acetoin / metabolism; Acetolactate Synthase / biosynthesis; Acetolactate Synthase / genetics; Catalysis; Ethanol / isolation & purification; Ethanol / metabolism; Gene Deletion; Genetic Enhancement / methods; Metabolic Engineering / methods; Metabolic Networks and Pathways / physiology; Pyrococcus furiosus / genetics; Pyrococcus furiosus / metabolism; Temperature
TL;DR: It is shown that P. furiosus produces acetoin (3-hydroxybutanone) as a major product at temperatures below 80°C and deletion of the als gene abolishes acetoin production, and that a strain of P.furiosus containing a novel pathway for ethanol production significantly improved the yield of ethanol. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2016 journal article

Transcriptomes of the Extremely Thermoacidophilic Archaeon Metallosphaera sedula Exposed to Metal "Shock" Reveal Generic and Specific Metal Responses

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 82(15), 4613–4627.

By: G. Wheaton n, A. Mukherjee n & R. Kelly n

Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2015 journal article

A New Class of Tungsten-Containing Oxidoreductase in Caldicellulosiruptor, a Genus of Plant Biomass-Degrading Thermophilic Bacteria

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 81(20), 7339–7347.

By: I. Scott n, G. Rubinstein n, G. Lipscomb n, M. Basen n, G. Schut n, A. Rhaesa n, W. Lancaster n, F. Poole n, R. Kelly n, M. Adams n

MeSH headings : Aldehydes / metabolism; Bacterial Proteins / metabolism; Gram-Positive Bacteria / enzymology; Gram-Positive Bacteria / metabolism; Oxidation-Reduction; Oxidoreductases / chemistry; Oxidoreductases / metabolism; Pyrococcus furiosus / enzymology; Pyrococcus furiosus / metabolism; Tungsten / metabolism
TL;DR: Evidence was uncovered to suggest that the primary redox metabolism of C. bescii has a completely uncharacterized aspect involving tungsten, a rarely used element in biology. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2015 journal article

A hybrid synthetic pathway for butanol production by a hyperthermophilic microbe

METABOLIC ENGINEERING, 27, 101–106.

By: M. Keller*, G. Lipscomb*, A. Loder n, G. Schut*, R. Kelly n & M. Adams*

author keywords: Butanol; Biofuels; Hyperthermophile; Module; Hybrid; Pathway
MeSH headings : 1-Butanol / metabolism; Acetyl Coenzyme A / genetics; Acetyl Coenzyme A / metabolism; Metabolic Engineering / methods; Thermoanaerobacterium / genetics; Thermoanaerobacterium / metabolism
TL;DR: A synthetic metabolic pathway assembled from genes obtained from three different sources for conversion of acetyl-CoA to 1-butanol was demonstrated near 70°C in a microorganism that grows optimally near 100°C, and the module could also be used in thermophiles capable of degrading plant biomass. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2015 journal article

A mutant ('lab strain') of the hyperthermophilic archaeon Pyrococcus furiosus, lacking flagella, has unusual growth physiology

EXTREMOPHILES, 19(2), 269–281.

By: D. Lewis n, J. Notey n, S. Chandrayan*, A. Loder n, G. Lipscomb*, M. Adams*, R. Kelly n

author keywords: Pyrococcus furiosus; Hyperthermophile; Archaeon; Growth physiology; Flagella
TL;DR: It is indicated that flagella play a key role, beyond motility, in the growth physiology of P. furiosus and, unlike the WT, showed minimal evidence of exopolysaccharide-based cell aggregation in post-exponential phase. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2015 journal article

Alcohol Selectivity in a Synthetic Thermophilic n-Butanol Pathway Is Driven by Biocatalytic and Thermostability Characteristics of Constituent Enzymes

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 81(20), 7187–7200.

By: A. Loder n, B. Zeldes n, G. Garrison n, G. Lipscomb*, M. Adams* & R. Kelly n

MeSH headings : 1-Butanol / metabolism; Acetyl-CoA C-Acyltransferase / metabolism; Acyl Coenzyme A / metabolism; Alcohol Dehydrogenase / metabolism; Alcohol Oxidoreductases / metabolism; Aldehyde Oxidoreductases / metabolism; Biocatalysis; Chromatography, Gas; Clostridium acetobutylicum / metabolism; Clostridium thermocellum / metabolism; Thermoanaerobacter / metabolism
TL;DR: More-selective thermophilic versions of Bad, Bdh, and AdhE are needed to fully exploit biocatalytic n-butanol production at elevated temperatures. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2015 journal article

Bioprocessing analysis of Pyrococcus furiosus strains engineered for CO2-based 3-hydroxypropionate production

BIOTECHNOLOGY AND BIOENGINEERING, 112(8), 1533–1543.

By: A. Hawkins n, H. Lian n, B. Zeldes n, A. Loder n, G. Lipscomb*, G. Schut*, M. Keller*, M. Adams*, R. Kelly n

author keywords: 3-hydroxypropionate; CO2 fixation; Metallosphaera sedula; Pyrococcus furiosus
MeSH headings : Bioreactors / microbiology; Carbon Dioxide / metabolism; Gene Expression Profiling; Hot Temperature; Lactic Acid / analogs & derivatives; Lactic Acid / metabolism; Maltose / metabolism; Metabolic Engineering / methods; Metabolic Networks and Pathways / genetics; Pyrococcus furiosus / genetics; Pyrococcus furiosus / metabolism; Pyrococcus furiosus / radiation effects; Sulfolobaceae / genetics
TL;DR: The results here support the prospects of using thermally diverse sources of pathways and enzymes in metabolically engineered strains designed for product formation at sub‐optimal growth temperatures. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2015 journal article

Comparative Analysis of Extremely Thermophilic Caldicellulosiruptor Species Reveals Common and Unique Cellular Strategies for Plant Biomass Utilization

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 81(20), 7159–7170.

By: J. Zurawski n, J. Conway n, L. Lee n, H. Simpson n, J. Izquierdo n, S. Blumer-Schuette n, I. Nookaew*, M. Adams*, R. Kelly n

MeSH headings : Bacteria / metabolism; Biomass; Cellulase / metabolism; Panicum / enzymology; Panicum / metabolism; Plants / enzymology
TL;DR: Results here show that capacity for plant biomass deconstruction varies across Caldicellulosiruptor species and depends in a complex way on GH genome inventory, substrate composition, and gene regulation. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2015 journal article

Complete Genome Sequences of Caldicellulosiruptor sp. Strain Rt8.B8, Caldicellulosiruptor sp. Strain Wai35.B1, and “ Thermoanaerobacter cellulolyticus ”

Complete Genome Sequences of Caldicellulosiruptor sp. Strain Rt8.B8, Caldicellulosiruptor sp. Strain Wai35.B1, and “ Thermoanaerobacter cellulolyticus .” Genome Announcements, 3(3).

By: L. Lee n, J. Izquierdo n, S. Blumer-Schuette n, J. Zurawski n, J. Conway n, R. Cottingham*, M. Huntemann*, A. Copeland* ...

TL;DR: Gains in genome sequences for three additional Caldicellulosiruptor species are reported, including Rt8.B8 DSM 8990 (New Zealand), Wai35.B1 DSM 8977 ( new Zealand), and “Thermoanaerobacter cellulolyticus” strain NA10 DSM 8991 (Japan). (via Semantic Scholar)
Sources: Crossref, NC State University Libraries
Added: July 21, 2021

2015 journal article

Discrete and Structurally Unique Proteins (Tapirins) Mediate Attachment of Extremely Thermophilic Caldicellulosiruptor Species to Cellulose

JOURNAL OF BIOLOGICAL CHEMISTRY, 290(17), 10645–10656.

By: S. Blumer-Schuette n, M. Alahuhta*, J. Conway n, L. Lee n, J. Zurawski n, R. Giannone*, R. Hettich*, V. Lunin, M. Himmel*, R. Kelly n

MeSH headings : Adsorption; Bacteria / genetics; Bacteria / metabolism; Bacteria / ultrastructure; Bacterial Adhesion / physiology; Bacterial Proteins / chemistry; Bacterial Proteins / genetics; Bacterial Proteins / metabolism; Binding Sites; Cellulose / metabolism; Fimbriae, Bacterial / metabolism; Genes, Bacterial; Models, Molecular; Phylogeny; Plants / microbiology; Protein Structure, Tertiary; Recombinant Proteins / chemistry; Recombinant Proteins / genetics; Recombinant Proteins / metabolism
TL;DR: Structurally unique, discrete proteins (tāpirins), not directly associated with cellulases, that mediate attachment to cellulose by species in the noncellulosomal, extremely thermophilic bacterial genus Caldicellulosiruptor are identified and define a new class of polysaccharide adhesins. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2015 review

Extremely thermophilic microorganisms as metabolic engineering platforms for production of fuels and industrial chemicals

[Review of ]. FRONTIERS IN MICROBIOLOGY, 6.

By: B. Zeldes n, M. Keller*, A. Loder n, C. Straub n, M. Adams* & R. Kelly n

author keywords: extreme thermophiles; metabolic engineering; bio-based chemicals; genetics; biotechnology
TL;DR: The issues and opportunities for extremely thermophilic metabolic engineering platforms are considered with an eye toward potential technological advantages for high temperature industrial biotechnology. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2015 report

Genetically engineered microbes and methods for converting organic acid to alcohols

(US Patent Application No. 14/670,601).

By: R. Kelly, M. Adams, G. Schut & M. Basen

Source: NC State University Libraries
Added: August 27, 2021

2015 journal article

Machine learning reveals sex-specific 17β-estradiol-responsive expression patterns in white perch (Morone americana) plasma proteins

PROTEOMICS, 15(15), 2678–2690.

By: J. Schilling n, A. Nepomuceno n, A. Planchart n, J. Yoder n, R. Kelly n, D. Muddiman n, H. Daniels n, N. Hiramatsu*, B. Reading n

Contributors: J. Schilling n, A. Nepomuceno n, A. Planchart n, J. Yoder n, R. Kelly n, D. Muddiman n, H. Daniels n, N. Hiramatsu*, B. Reading n

author keywords: Animal proteomics; Endocrine disrupting compounds; Machine learning; Plasma; Semiquantitative proteomics; Support vector machines
MeSH headings : Animals; Bass / metabolism; Blood Proteins / metabolism; Blotting, Western; Chromatography, Liquid; Estradiol / pharmacology; Estrogens / pharmacology; Female; Fish Proteins / metabolism; Kininogens / metabolism; Male; Nanotechnology / methods; Proteome / drug effects; Proteome / metabolism; Proteomics / methods; Sex Factors; Support Vector Machine; Tandem Mass Spectrometry; Vitellogenins / metabolism
TL;DR: This is the first report that Eltd1 and Kng1 may be E2‐responsive proteins in fishes and therefore may be useful indicators of estrogen induction in fishes. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries, ORCID, Crossref
Added: August 6, 2018

2015 review

The Confluence of Heavy Metal Biooxidation and Heavy Metal Resistance: Implications for Bioleaching by Extreme Thermoacidophiles

[Review of ]. MINERALS, 5(3), 397–451.

By: G. Wheaton n, J. Counts n, A. Mukherjee n, J. Kruh n & R. Kelly n

author keywords: extreme thermoacidophiles; bioleaching; heavy metal resistance; heavy metal biooxidation; archaea
TL;DR: Here, extreme thermoacidophiles are examined from the perspectives of biodiversity, heavy metal biooxidation, metal resistance mechanisms, microbe-solid interactions, and application of these archaea in biomining operations. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2014 journal article

Conversion of 4-Hydroxybutyrate to Acetyl Coenzyme A and Its Anapleurosis in the Metallosphaera sedula 3-Hydroxypropionate/4-Hydroxybutyrate Carbon Fixation Pathway

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 80(8), 2536–2545.

By: A. Hawkins n, M. Adams* & R. Kelly n

MeSH headings : Acetyl Coenzyme A / metabolism; Carbon Cycle; Escherichia coli / genetics; Gene Expression Profiling; Metabolic Networks and Pathways / genetics; Recombinant Proteins / genetics; Recombinant Proteins / isolation & purification; Recombinant Proteins / metabolism; Sodium Oxybate / metabolism; Sulfolobaceae / metabolism; Transcriptome
TL;DR: Transcriptomic analysis revealed the importance of the 3HP/4HB pathway in supplying acetyl-CoA to anabolic pathways generating intermediates in M. sedula metabolism, and provided the framework for examining connections between CO2 fixation and central metabolism in the sedula. (via Semantic Scholar)
UN Sustainable Development Goal Categories
6. Clean Water and Sanitation (OpenAlex)
15. Life on Land (Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2014 journal article

Cross-linked Polymer Nanofibers for Hyperthermophilic Enzyme Immobilization: Approaches to Improve Enzyme Performance

ACS APPLIED MATERIALS & INTERFACES, 6(15), 11899–11906.

By: C. Tang n, C. Saquing n, S. Morton n, B. Glatz n, R. Kelly n & S. Khan n

author keywords: enzyme immobilization; nanofibers; electrospinning; biocatalysts; mass transfer
MeSH headings : Catalysis; Cross-Linking Reagents / chemistry; Electrochemistry; Enzymes, Immobilized / chemistry; Escherichia coli / metabolism; Hydrogen-Ion Concentration; Kinetics; Microscopy, Electron, Scanning; Nanofibers / chemistry; Nanotechnology / methods; Polymers / chemistry; Polyvinyl Alcohol / chemistry; Solvents / chemistry; Temperature; Thermotoga maritima / metabolism
TL;DR: An enzyme immobilization method effective at elevated temperatures (up to 105 °C) and sufficiently robust for hyperthermophilic enzymes is reported and Improvements in the apparent activity can be achieved by incorporating a cryoprotectant during immobilization to prevent enzyme deactivation. (via Semantic Scholar)
UN Sustainable Development Goal Categories
6. Clean Water and Sanitation (OpenAlex)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2014 journal article

Deletion of acetyl-CoA synthetases I and II increases production of 3-hydroxypropionate by the metabolically-engineered hyperthermophile Pyrococcus furiosus

METABOLIC ENGINEERING, 22, 83–88.

By: M. Thorgersen*, G. Lipscomb*, G. Schut*, R. Kelly n & M. Adams*

author keywords: Electrofuels; Metallosphaera sedula; Acetyl-CoA synthetase; 3-Hydroxypropionate
MeSH headings : Archaeal Proteins; Coenzyme A Ligases; Gene Deletion; Lactic Acid / analogs & derivatives; Lactic Acid / metabolism; Metabolic Engineering / methods; Pyrococcus furiosus / genetics; Pyrococcus furiosus / metabolism
TL;DR: It is shown that strains of MW56 lacking the α-subunit of either of the two ACSs previously characterized from P. furiosus exhibit a three-fold increase in specific 3-HP production. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2014 journal article

Engineering Hydrogen Gas Production from Formate in a Hyperthermophile by Heterologous Production of an 18-Subunit Membrane-bound Complex

JOURNAL OF BIOLOGICAL CHEMISTRY, 289(5), 2873–2879.

By: G. Lipscomb*, G. Schut*, M. Thorgersen*, W. Nixon*, R. Kelly n & M. Adams*

author keywords: Archaea; Enzymes; Genetics; Metabolic Engineering; Microbiology; Hydrogen; Hyperthermophiles
MeSH headings : Biofuels / microbiology; Formates / metabolism; Gases / metabolism; Genes, Bacterial / genetics; Hydrogen / metabolism; Membrane Proteins / genetics; Membrane Proteins / metabolism; Metabolic Engineering / methods; Multienzyme Complexes / genetics; Multienzyme Complexes / metabolism; Operon / genetics; Pyrococcus furiosus / enzymology; Pyrococcus furiosus / genetics; Thermococcus / enzymology; Thermococcus / genetics
TL;DR: The model hyperthermophile Pyrococcus furiosus, which grows optimally near 100 °C by fermenting sugars to produce H2, has been engineered to also efficiently convert formate to H1, demonstrating the versatility of this model organism for metabolic engineering purposes. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2014 journal article

Nanofibrous membranes for single-step immobilization of hyperthermophilic enzymes

JOURNAL OF MEMBRANE SCIENCE, 472, 251–260.

By: C. Tang n, C. Saquing n, P. Sarin n, R. Kelly n & S. Khan n

author keywords: Enzyme immobilization; Nanofibers; Electrospinning; Chemical crosslinking; Hyperthermophilic enzymes
TL;DR: Analysis of this immobilization method indicates that the apparent decrease in specific activity could be attributed to enzyme deactivation arising from the crosslinking reaction, whereas mass transfer limits the apparent activity using alternative two-step immobilization methods. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2014 journal article

Production of lignofuels and electrofuels by extremely thermophilic microbes

Biofuels, 5(5), 499–515.

By: M. Keller*, A. Loder n, M. Basen*, J. Izquierdo n, R. Kelly n & M. Adams*

author keywords: extreme thermophiles; Pyrococcus furiosus; Metallosphaera sedula; Caldicellulosiruptor; biofuels; electrofuels; CO2 fixation; lignocellulose
TL;DR: Recent progress towards the synthesis of lignofuels and electrofuels by extremely thermophilic microorganisms is reviewed, including temperature-regulated biosynthesis of industrial organic chemicals and liquid fuel molecules. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Sources: Crossref, NC State University Libraries
Added: February 24, 2020

2014 journal article

Role of an Archaeal PitA Transporter in the Copper and Arsenic Resistance of Metallosphaera sedula, an Extreme Thermoacidophile

JOURNAL OF BACTERIOLOGY, 196(20), 3562–3570.

By: S. McCarthy*, C. Ai*, G. Wheaton n, R. Tevatia*, V. Eckrich*, R. Kelly n, P. Blum*

MeSH headings : Archaeal Proteins / genetics; Archaeal Proteins / metabolism; Arsenic / metabolism; Arsenic / toxicity; Biological Transport; Copper / metabolism; Copper / toxicity; Gene Expression Regulation, Archaeal / physiology; Genome, Archaeal; Molecular Sequence Data; Sulfolobaceae / drug effects; Sulfolobaceae / metabolism
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2014 journal article

Single gene insertion drives bioalcohol production by a thermophilic archaeon

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 111(49), 17618–17623.

By: M. Basen*, G. Schut*, D. Nguyen*, G. Lipscomb*, R. Benn*, C. Prybol*, B. Vaccaro*, F. Poole*, R. Kelly n, M. Adams*

author keywords: Archaea; metabolic engineering; hyperthermophile; carbon monoxide; aldehydes
MeSH headings : Acetates / chemistry; Aldehyde Oxidoreductases / genetics; Aldehyde Oxidoreductases / metabolism; Aldehydes / chemistry; Biofuels; Carbon Monoxide / chemistry; Escherichia coli / metabolism; Ethanol / chemistry; Fermentation; Maltose / chemistry; Mutagenesis, Insertional; Protein Engineering / methods; Pyrococcus furiosus / genetics; Temperature
TL;DR: This study engineered the model hyperthermophile Pyrococcus furiosus to produce various alcohols from their corresponding organic acids by constructing a synthetic route termed the AOR/AdhA pathway, a potentially game-changing strategy for syngas fermentation, especially in combination with carbon chain elongation pathways. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2014 chapter

The Extremely Thermophilic Genus Caldicellulosiruptor: Physiological and Genomic Characteristics for Complex Carbohydrate Conversion to Molecular Hydrogen

In Microbial BioEnergy: Hydrogen Production (pp. 177–195).

By: J. Zurawski n, S. Blumer-Schuette n, J. Conway n & R. Kelly n

TL;DR: Extremely thermophilic, carbohydrate-utilizing bacteria from the genus Caldicellulosiruptor should be considered for biohydrogen production to take advantage of their broad growth substrate range and high substrate conversion efficiency. (via Semantic Scholar)
Sources: Crossref, NC State University Libraries
Added: July 21, 2021

2014 chapter

The Order Thermococcales and the Family Thermococcaceae

In The Prokaryotes (pp. 363–383).

By: G. Schut*, G. Lipscomb*, Y. Han n, J. Notey n, R. Kelly n & M. Adams*

Sources: Crossref, NC State University Libraries
Added: July 21, 2021

2014 review

Thermophilic lignocellulose deconstruction

[Review of ]. FEMS MICROBIOLOGY REVIEWS, 38(3), 393–448.

author keywords: thermophilic microorganisms; cellulosome; lignocellulose deconstruction; carbohydrate-active enzymes; systems biology; bioenergy
MeSH headings : Biofuels; Cellulosomes / genetics; Cellulosomes / metabolism; Gram-Positive Endospore-Forming Bacteria / classification; Gram-Positive Endospore-Forming Bacteria / enzymology; Gram-Positive Endospore-Forming Bacteria / genetics; Hot Temperature; Lignin / metabolism; Molecular Sequence Data; Plants / metabolism; Soil Microbiology
TL;DR: Plant-biomass-deconstructing thermophilic bacteria from the genera Clostridium and Caldicellulosiruptor, which have potential as metabolic engineering platforms for producing biofuels, are compared and contrasted from a systems biology perspective. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2013 journal article

A thermophile under pressure: Transcriptional analysis of the response of Caldicellulosiruptor saccharolyticus to different H-2 partial pressures

INTERNATIONAL JOURNAL OF HYDROGEN ENERGY, 38(4), 1837–1849.

By: A. Bielen*, M. Verhaart*, A. VanFossen n, S. Blumer-Schuette n, A. Stams*, J. Oost*, R. Kelly n, S. Kengen*

author keywords: Caldicellulosiruptor saccharolyticus; Hydrogen partial pressure; Chemostat; Metabolic shift; Gene expression; Rex transcriptional regulator
TL;DR: Transmission data provide evidence for the involvement of the redox sensing Rex protein in gene regulation under high P H 2 cultivation conditions and differential transcription was observed for genes involved in carbon metabolism, fatty acid biosynthesis and several transport systems. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2013 review

Biological conversion of carbon dioxide and hydrogen into liquid fuels and industrial chemicals

[Review of ]. CURRENT OPINION IN BIOTECHNOLOGY, 24(3), 376–384.

By: A. Hawkins n, P. McTernan*, H. Lian n, R. Kelly n & M. Adams*

MeSH headings : Bioelectric Energy Sources; Biotechnology / methods; Carbon Cycle / genetics; Carbon Dioxide / metabolism; Chemical Industry; Hydrogen / metabolism; Metabolic Engineering / methods
TL;DR: This review examines the current landscape of electrofuel projects with a focus on hydrogen-utilizing organisms covering the biochemistry of hydrogenases and carbonic anhydrases, kinetic and energetic analyses of the known carbon fixation pathways, and the state of genetic systems for current and prospective electrofuel-producing microorganisms. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2013 journal article

Carbohydrate and lignin are simultaneously solubilized from unpretreated switchgrass by microbial action at high temperature

Energy & Environmental Science, 6(7), 2186.

By: I. Kataeva*, M. Foston*, S. Yang*, S. Pattathil*, A. Biswal*, F. Poole II, M. Basen*, A. Rhaesa* ...

TL;DR: This unexpected finding of simultaneous lignin and carbohydrate solubilization bodes well for industrial conversion by extremely thermophilic microbes of biomass that requires limited or, more importantly, no chemical pretreatment. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Source: Crossref
Added: February 24, 2020

2013 journal article

Exploiting microbial hyperthermophilicity to produce an industrial chemical, using hydrogen and carbon dioxide

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 110(15), 5840–5845.

By: M. Keller*, G. Schut*, G. Lipscomb*, A. Menon*, I. Iwuchukwu*, T. Leuko*, M. Thorgersen*, W. Nixon* ...

author keywords: anaerobe; archaea; biotechnology; metabolic engineering; thermophile
MeSH headings : Carbohydrates / chemistry; Carbon Dioxide / chemistry; Gases; Genetic Engineering; Hydrogen / chemistry; Industrial Microbiology / methods; Lactic Acid / analogs & derivatives; Lactic Acid / biosynthesis; Lactic Acid / chemistry; Operon; Polymerase Chain Reaction; Pyrococcus furiosus / genetics; Pyrococcus furiosus / growth & development; Pyrococcus furiosus / metabolism; Temperature
TL;DR: A unique temperature-dependent approach is described that confers on a microorganism (the archaeon Pyrococcus furiosus, which grows optimally on carbohydrates at 100°C) the capacity to use carbon dioxide, a reaction that it does not accomplish naturally. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2013 journal article

Role of 4-Hydroxybutyrate-CoA Synthetase in the CO2 Fixation Cycle in Thermoacidophilic Archaea

JOURNAL OF BIOLOGICAL CHEMISTRY, 288(6), 4012–4022.

By: A. Hawkins n, Y. Han n, R. Bennett n, M. Adams* & R. Kelly n

MeSH headings : Archaeal Proteins / chemistry; Archaeal Proteins / genetics; Archaeal Proteins / metabolism; Carbon Dioxide / metabolism; Catalysis; Catalytic Domain; Coenzyme A-Transferases / chemistry; Coenzyme A-Transferases / genetics; Coenzyme A-Transferases / metabolism; Genome, Archaeal / physiology; Hydroxybutyrates / chemistry; Hydroxybutyrates / metabolism; Sulfolobaceae / enzymology; Sulfolobaceae / genetics
TL;DR: Transcriptomic analysis of cells grown under strict H2-CO2 autotrophy was consistent with the involvement of Msed_0406 and Msed-0394, and the identification of the 4-HB CoA synthetase now completes the set of enzymes comprising the 3HP/4HB cycle. (via Semantic Scholar)
UN Sustainable Development Goal Categories
15. Life on Land (Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2013 journal article

Stationary Phase and Nutrient Levels Trigger Transcription of a Genomic Locus Containing a Novel Peptide (TM1316) in the Hyperthermophilic Bacterium Thermotoga maritima

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 79(21), 6637–6646.

By: A. Frock n, C. Montero n, S. Blumer-Schuette n & R. Kelly n

MeSH headings : Carbon / metabolism; Gene Expression Regulation, Bacterial / genetics; Gene Expression Regulation, Bacterial / physiology; Genetic Loci / genetics; Microscopy, Fluorescence; Molecular Sequence Annotation / methods; Nitrogen / metabolism; Operon / genetics; Peptides / genetics; Thermotoga maritima / genetics; Thermotoga maritima / growth & development; Thermotoga maritima / metabolism
TL;DR: The onset of stationary phase in T. maritima was accompanied by significant morphological changes and upregulation of several ORFs located in the TM1298-TM1336 genome locus, which includes a putative 31-amino-acid peptide (TM1316) that was the most highly transcribed gene in the transcriptome during stationary phase. (via Semantic Scholar)
UN Sustainable Development Goal Categories
14. Life Below Water (OpenAlex)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2012 journal article

Caldicellulosiruptor Core and Pangenomes Reveal Determinants for Noncellulosomal Thermophilic Deconstruction of Plant Biomass

JOURNAL OF BACTERIOLOGY, 194(15), 4015–4028.

By: S. Blumer-Schuette n, R. Giannone*, J. Zurawski n, I. Ozdemir n, Q. Ma*, Y. Yin*, Y. Xu*, I. Kataeva* ...

MeSH headings : Adhesins, Bacterial / analysis; Adhesins, Bacterial / genetics; Biomass; Carbohydrate Metabolism; Cellulases / analysis; Cellulases / genetics; Cellulose / metabolism; Genetic Variation; Genome, Bacterial; Gram-Positive Bacteria / enzymology; Gram-Positive Bacteria / genetics; Metabolic Networks and Pathways / genetics; Plants / chemistry; Proteome / analysis
TL;DR: Taking into account the core genomes, pangenomes, and individual genomes, the ancestral Caldicellulosiruptor was likely cellulolytic and evolved, in some cases, into species that lost the ability to degrade crystalline cellulose while maintaining the capacity to hydrolyze amorphous cellulose and hemICEllulose. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2012 journal article

Epimerase (Msed_0639) and Mutase (Msed_0638 and Msed_2055) Convert (S)-Methylmalonyl-Coenzyme A (CoA) to Succinyl-CoA in the Metallosphaera sedula 3-Hydroxypropionate/4-Hydroxybutyrate Cycle

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 78(17), 6194–6202.

By: Y. Han n, A. Hawkins n, M. Adams* & R. Kelly n

MeSH headings : Acyl Coenzyme A / metabolism; Autotrophic Processes; Biotransformation; Carbon Dioxide / metabolism; Cobamides / metabolism; Escherichia coli / genetics; Gene Expression; Gene Expression Profiling; Heterotrophic Processes; Hydroxybutyrates / metabolism; Methylmalonyl-CoA Mutase / chemistry; Methylmalonyl-CoA Mutase / genetics; Methylmalonyl-CoA Mutase / metabolism; Protein Multimerization; Racemases and Epimerases / chemistry; Racemases and Epimerases / genetics; Racemases and Epimerases / metabolism; Recombinant Proteins / genetics; Recombinant Proteins / metabolism; Sulfolobaceae / enzymology; Sulfolobaceae / genetics; Sulfolobaceae / metabolism
TL;DR: Based on the highly conserved occurrence of single copies of MCE and MCM in Sulfolobaceae genomes, the M. sedula enzymes are likely to be representatives of these enzymes in the 3-HP/4-HB cycle in crenarchaeal thermoacidophiles. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2012 journal article

Extreme thermophiles: moving beyond single-enzyme biocatalysis

Current Opinion in Chemical Engineering, 1(4), 363–372.

By: A. Frock n & R. Kelly n

TL;DR: Although the pace for discovering novel microorganisms has slowed over the past two decades, genome sequence data have provided clues to novel biomolecules and metabolic pathways, which can be mined for a range of new applications. (via Semantic Scholar)
Sources: Crossref, NC State University Libraries
Added: February 24, 2020

2012 journal article

Hyperthermophilic Thermotoga Species Differ with Respect to Specific Carbohydrate Transporters and Glycoside Hydrolases

Applied and Environmental Microbiology, 78(6), 1978–1986.

By: A. Frock n, S. Gray n & R. Kelly n

MeSH headings : ATP-Binding Cassette Transporters / genetics; ATP-Binding Cassette Transporters / metabolism; Bacterial Typing Techniques; Carbohydrate Metabolism; Gene Expression Profiling; Glycoside Hydrolases / genetics; Glycoside Hydrolases / metabolism; Gram-Negative Anaerobic Straight, Curved, and Helical Rods / classification; Gram-Negative Anaerobic Straight, Curved, and Helical Rods / enzymology; Gram-Negative Anaerobic Straight, Curved, and Helical Rods / genetics; Gram-Negative Anaerobic Straight, Curved, and Helical Rods / metabolism; Microarray Analysis
TL;DR: Results here show that subtle differences exist among the hyperthermophilic Thermotogales with respect to carbohydrate utilization, which supports their designation as separate species. (via Semantic Scholar)
UN Sustainable Development Goal Categories
15. Life on Land (Web of Science)
Sources: Crossref, NC State University Libraries
Added: August 3, 2021

2012 journal article

S-Layer Homology Domain Proteins Csac_0678 and Csac_2722 Are Implicated in Plant Polysaccharide Deconstruction by the Extremely Thermophilic Bacterium Caldicellulosiruptor saccharolyticus

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 78(3), 768–777.

By: I. Ozdemir n, S. Blumer-Schuette n & R. Kelly n

MeSH headings : Enzyme Stability; Glycoside Hydrolases / chemistry; Glycoside Hydrolases / genetics; Glycoside Hydrolases / metabolism; Gram-Positive Bacteria / enzymology; Gram-Positive Bacteria / genetics; Gram-Positive Bacteria / metabolism; Hydrogen-Ion Concentration; Membrane Glycoproteins / chemistry; Membrane Glycoproteins / genetics; Membrane Glycoproteins / metabolism; Molecular Weight; Open Reading Frames; Plants / microbiology; Polysaccharides / metabolism; Protein Binding; Sequence Deletion; Temperature
TL;DR: Roles for Csac-0678 and Csac_2722 in recruitment and hydrolysis of complex polysaccharides and the deconstruction of lignocellulosic biomass are indicated and related SLH domain proteins in other Caldicellulosiruptor genomes may also be important contributors to plant biomass utilization. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2012 journal article

Uranium extremophily is an adaptive, rather than intrinsic, feature for extremely thermoacidophilic Metallosphaera species

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 109(41), 16702–16707.

By: A. Mukherjee n, G. Wheaton n, P. Blum* & R. Kelly n

author keywords: uranium oxidation; uranium resistance; chemolithotrophy; terminal oxidases
MeSH headings : Adaptation, Physiological / drug effects; Adaptation, Physiological / genetics; Archaeal Proteins / genetics; Archaeal Proteins / metabolism; Electrophoresis, Gel, Two-Dimensional; Environmental Pollutants / chemistry; Environmental Pollutants / toxicity; Gene Expression Regulation, Archaeal / drug effects; Mutation; RNA Stability / drug effects; RNA, Archaeal / genetics; RNA, Archaeal / metabolism; Species Specificity; Sulfolobaceae / classification; Sulfolobaceae / genetics; Sulfolobaceae / metabolism; Time Factors; Transcriptome / genetics; Uranium / chemistry; Uranium / toxicity
TL;DR: The results point to uranium extremophily as an adaptive, rather than intrinsic, feature for Metallosphaera species, driven by environmental factors. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2011 journal article

A novel alpha-d-galactosynthase from Thermotoga maritima converts beta-d-galactopyranosyl azide to alpha-galacto-oligosaccharides

GLYCOBIOLOGY, 21(4), 448–456.

author keywords: alpha-galacto-oligosaccharides; glycosynthase; alpha-gal epitope; carbohydrate synthesis; protein engineering
MeSH headings : Aspartic Acid / chemistry; Azides / chemistry; Chromatography, Thin Layer; Disaccharides / biosynthesis; Galactose / metabolism; Glycine / chemistry; Kinetics; Mutagenesis, Site-Directed; Mutant Proteins / chemistry; Mutant Proteins / genetics; Mutant Proteins / metabolism; Protein Engineering; Thermotoga maritima / enzymology; alpha-Galactosidase / chemistry; alpha-Galactosidase / genetics; alpha-Galactosidase / metabolism
TL;DR: Results open a new avenue to the practical synthesis of biologically interesting α-galacto-oligosaccharides and demonstrate more widespread use of β-glycosyl-azide as donors, confirming their utility to expand the repertoire of glycosynthases. (via Semantic Scholar)
UN Sustainable Development Goal Categories
14. Life Below Water (OpenAlex)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2011 journal article

Complete Genome Sequences for the Anaerobic, Extremely Thermophilic Plant Biomass-Degrading Bacteria Caldicellulosiruptor hydrothermalis , Caldicellulosiruptor kristjanssonii , Caldicellulosiruptor kronotskyensis , Caldicellulosiruptor owensensis , and Caldicellulosiruptor lactoaceticus

Journal of Bacteriology, 193(6), 1483–1484.

By: S. Blumer-Schuette n, I. Ozdemir n, D. Mistry n, S. Lucas*, A. Lapidus*, J. Cheng*, L. Goodwin*, S. Pitluck* ...

MeSH headings : DNA, Bacterial / chemistry; DNA, Bacterial / genetics; Genetic Variation; Genome, Bacterial; Glycoside Hydrolases / genetics; Gram-Positive Bacteria / genetics; Membrane Transport Proteins / genetics; Molecular Sequence Data; Sequence Analysis, DNA
TL;DR: Seven completed and one draft-phase Caldicellulosiruptor genomes suggest that, while central metabolism is highly conserved, significant differences in glycoside hydrolase inventories and numbers of carbohydrate transporters exist, a finding which likely relates to variability observed in plant biomass degradation capacity. (via Semantic Scholar)
Sources: Crossref, NC State University Libraries
Added: July 21, 2021

2011 journal article

Extremely Thermophilic Routes to Microbial Electrofuels

ACS CATALYSIS, 1(9), 1043–1050.

By: A. Hawkins n, Y. Han n, H. Lian n, A. Loder n, A. Menon*, I. Iwuchukwu*, M. Keller*, T. Leuko*, M. Adams*, R. Kelly n

UN Sustainable Development Goal Categories
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2011 journal article

Glycoside Hydrolase Inventory Drives Plant Polysaccharide Deconstruction by the Extremely Thermophilic Bacterium Caldicellulosiruptor saccharolyticus

BIOTECHNOLOGY AND BIOENGINEERING, 108(7), 1559–1569.

By: A. VanFossen n, I. Ozdemir n, S. Zelin n & R. Kelly n

author keywords: Caldicellulosiruptor saccharolyticus; extreme thermophile; plant biomass; glycoside hydrolases
MeSH headings : Bacterial Proteins / chemistry; Bacterial Proteins / genetics; Bacterial Proteins / metabolism; Carboxymethylcellulose Sodium / metabolism; Culture Media / chemistry; Gene Expression Profiling; Glycoside Hydrolases / chemistry; Glycoside Hydrolases / genetics; Glycoside Hydrolases / metabolism; Gram-Positive Bacteria / enzymology; Gram-Positive Bacteria / genetics; Gram-Positive Bacteria / metabolism; Molecular Weight; Plants / chemistry; Polysaccharides / metabolism; Xylans / metabolism
UN Sustainable Development Goal Categories
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2011 journal article

Insights into plant biomass conversion from the genome of the anaerobic thermophilic bacterium Caldicellulosiruptor bescii DSM 6725

NUCLEIC ACIDS RESEARCH, 39(8), 3240–3254.

By: P. Dam n, I. Kataeva n, S. Yang n, F. Zhou n, Y. Yin n, W. Chou n, F. Poole n, J. Westpheling n ...

MeSH headings : Bacterial Adhesion; Bacterial Proteins / genetics; Biomass; Carbohydrate Metabolism / genetics; Gene Expression Profiling; Genes, Bacterial; Genome, Bacterial; Genomics; Gram-Positive Bacteria / genetics; Gram-Positive Bacteria / metabolism; Gram-Positive Bacteria / ultrastructure; Plants / metabolism; Proteomics
TL;DR: The high duplication of CAZy domains coupled with the ability to acquire foreign genes by LGT may have allowed the bacterium to rapidly adapt to changing plant biomass-rich environments. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2011 journal article

Starch Self-Processing in Transgenic Sweet Potato Roots Expressing a Hyperthermophilic alpha-Amylase

BIOTECHNOLOGY PROGRESS, 27(2), 351–359.

By: M. Santa-Maria n, C. Yencho n, C. Haigler n, W. Thompson n, R. Kelly n & B. Sosinski n

author keywords: hyperthermophilic enzymes; starch conversion; transgenic plants; sweet potato; biofuels
MeSH headings : Crops, Agricultural / genetics; Hot Temperature; Ipomoea batatas / genetics; Ipomoea batatas / metabolism; Plant Roots / metabolism; Plants, Genetically Modified / enzymology; Plants, Genetically Modified / metabolism; Southeastern United States; Starch / metabolism; Thermotoga maritima / enzymology; alpha-Amylases / genetics; alpha-Amylases / physiology
TL;DR: The results presented here demonstrate that engineering plants with hyperthermophilic glycoside hydrolases can facilitate cost effective starch conversion to fermentable sugars and the use of sweet potato as an alternative near‐term energy crop should be considered. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries, ORCID
Added: August 6, 2018

2011 journal article

VapC6, a ribonucleolytic toxin regulates thermophilicity in the crenarchaeote Sulfolobus solfataricus

RNA, 17(7), 1381–1392.

By: Y. Maezato, A. Daugherty, K. Dana, E. Soo, C. Cooper, S. Tachdjian, R. Kelly*, P. Blum

author keywords: antitoxin; RNA degradation; heat shock; archaea; transcription
MeSH headings : Adaptation, Physiological / genetics; Adaptation, Physiological / physiology; Cell Line; Gene Expression Profiling; Gene Expression Regulation, Archaeal; Hydrolysis / drug effects; Models, Biological; RNA / drug effects; RNA / metabolism; Sulfolobus solfataricus / genetics; Sulfolobus solfataricus / metabolism; Sulfolobus solfataricus / physiology; Temperature; Toxins, Biological / genetics; Toxins, Biological / metabolism; Toxins, Biological / pharmacology; Toxins, Biological / physiology
TL;DR: The inactivation of the vapBC6 operon by targeted gene disruption produced two recessive phenotypes related to fitness, HS sensitivity and a heat-dependent reduction in the rate of growth that provide a biochemical mechanism and identify cellular targets underlying VapBC6-mediated control over microbial growth and survival at temperature extremes. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2010 chapter

Enzymes, Extremely Thermophilic

In M. C. Flickinger (Ed.), Encyclopedia of Industrial Biotechnology.

By: J. Harris n, M. Adams* & R. Kelly n

Ed(s): M. Flickinger

TL;DR: The availability of enzymes with optimal functional temperatures above 70°C has had considerable impact on basic and applied elements of biocatalysis and there is still much to be learned to appreciate this complex biomolecular trait. (via Semantic Scholar)
Sources: Crossref, NC State University Libraries
Added: August 3, 2021

2010 chapter

Extremely thermophilic microorganisms

In M. C. Flickinger (Ed.), Encyclopedia of Industrial Biotechnology: Bioprocess, Bioseparation, and Cell Technology (2nd ed., Vol. 5, pp. 3481–3499). NY: John Wiley and Sons.

By: S. Gray, M. Adams & R. Kelly

Ed(s): M. Flickinger

Source: NC State University Libraries
Added: August 27, 2021

2010 entry reference

Extremophiles

(I. T. Horváth, Ed.).

By: I. Ozdemir n & R. Kelly n

Ed(s): I. Horváth

Sources: Crossref, NC State University Libraries, NC State University Libraries
Added: July 14, 2021

2010 journal article

Impact of Molecular Hydrogen on Chalcopyrite Bioleaching by the Extremely Thermoacidophilic Archaeon Metallosphaera sedula

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 76(8), 2668–2672.

By: K. Auernik n & R. Kelly n

MeSH headings : Archaeal Proteins / biosynthesis; Copper / metabolism; Electron Transport Chain Complex Proteins / biosynthesis; Gene Expression Profiling; Hydrogen / metabolism; Sulfolobaceae / metabolism
TL;DR: Hydrogen served as a competitive inorganic energy source, impacting the CuFeS2 bioleaching efficiency of the extremely thermoacidophilic archaeon Metallosphaera sedula, and open reading frames encoding key terminal oxidase and electron transport chain components were triggered by Cu FeS2. (via Semantic Scholar)
UN Sustainable Development Goal Categories
7. Affordable and Clean Energy (OpenAlex)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2010 journal article

N-terminal Fusion of a Hyperthermophilic Chitin-Binding Domain to Xylose Isomerase from Thermotoga neapolitana Enhances Kinetics and Thermostability of Both Free and Immobilized Enzymes

BIOTECHNOLOGY PROGRESS, 26(4), 993–1000.

By: J. Harris n, K. Epting n & R. Kelly n

author keywords: xylose isomerase; hyperthermophile; Thermotoga neapolitana; chitin-binding domain
MeSH headings : Aldose-Ketose Isomerases / genetics; Aldose-Ketose Isomerases / metabolism; Chitin / metabolism; Enzyme Stability; Enzymes, Immobilized / genetics; Enzymes, Immobilized / metabolism; Kinetics; Protein Binding; Protein Structure, Tertiary / genetics; Protein Structure, Tertiary / physiology; Recombinant Proteins / genetics; Recombinant Proteins / metabolism; Thermotoga neapolitana / enzymology
TL;DR: Molecular modeling results suggest that the N‐terminal fusion impacted subunit interactions, thereby contributing to the enhanced thermostability of both the unbound and immobilized CBD‐TNXI, and played a role in modifying active site structure. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2010 journal article

Part I: characterization of the extracellular proteome of the extreme thermophile Caldicellulosiruptor saccharolyticus by GeLC-MS2

ANALYTICAL AND BIOANALYTICAL CHEMISTRY, 398(1), 377–389.

By: D. Muddiman n, G. Andrews n, D. Lewis n, J. Notey n & R. Kelly n

author keywords: Secretome; Caldicellulosiruptor saccharolyticus; GeLC-MS2; Thermophile; Sample preparation
MeSH headings : Bacterial Proteins / analysis; Bacterial Proteins / metabolism; Chromatography, Liquid; Electrophoresis, Polyacrylamide Gel; Extracellular Space / metabolism; Gram-Positive Bacteria / metabolism; Proteome / analysis; Spectrometry, Mass, Electrospray Ionization; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; Temperature
TL;DR: In an effort to define the extracellular proteome of the extreme thermophilic bacterium Caldicellulosiruptor saccharolyticus, several techniques were considered regarding sample processing to achieve the most in-depth analysis of secreted proteins. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2010 article

Part I: characterization of the extracellular proteome of the extreme thermophile Caldicellulosiruptor saccharolyticus by GeLC-MS2 (vol 398, pg 377, 2010)

Andrews, G., Lewis, D., Notey, J., Kelly, R., & Muddiman, D. (2010, October). ANALYTICAL AND BIOANALYTICAL CHEMISTRY, Vol. 398, pp. 1837–1837.

By: G. Andrews n, D. Lewis n, J. Notey n, R. Kelly n & D. Muddiman*

Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2010 journal article

Part II: defining and quantifying individual and co-cultured intracellular proteomes of two thermophilic microorganisms by GeLC-MS2 and spectral counting

ANALYTICAL AND BIOANALYTICAL CHEMISTRY, 398(1), 391–404.

By: D. Muddiman n, G. Andrews n, D. Lewis n, J. Notey n & R. Kelly n

author keywords: Thermotoga maritima; Caldicellulosiruptor saccharolyticus; GeLC-MS2; Co-cultivation; Intracellular proteome; Spectral counting
MeSH headings : Bacterial Proteins / analysis; Bacterial Proteins / metabolism; Chemotaxis; Chromatography, Liquid; Coculture Techniques; Electrophoresis, Polyacrylamide Gel; Fourier Analysis; Gram-Positive Bacteria / growth & development; Gram-Positive Bacteria / metabolism; Intracellular Space / metabolism; Proteome / analysis; Spectrometry, Mass, Electrospray Ionization; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; Spectrum Analysis / statistics & numerical data; Temperature; Thermotoga maritima / growth & development; Thermotoga maritima / metabolism
TL;DR: Altering in growth conditions of these bacterial thermophilic microorganisms offer a glimpse into the intricacy of microbial behavior and interaction as well as insight into microbial interaction and behavior. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2010 article

Part II: defining and quantifying individual and co-cultured intracellular proteomes of two thermophilic microorganisms by GeLC-MS2 and spectral counting (vol 398, pg 391, 2010)

Andrews, G., Lewis, D., Notey, J., Kelly, R., & Muddiman, D. (2010, October). ANALYTICAL AND BIOANALYTICAL CHEMISTRY, Vol. 398, pp. 1839–1839.

By: G. Andrews n, D. Lewis n, J. Notey n, R. Kelly n & D. Muddiman n

Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2010 journal article

Phylogenetic, Microbiological, and Glycoside Hydrolase Diversities within the Extremely Thermophilic, Plant Biomass-Degrading Genus Caldicellulosiruptor

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 76(24), 8084–8092.

By: S. Blumer-Schuette n, D. Lewis n & R. Kelly n

MeSH headings : Bacterial Proteins / analysis; Biomass; Carbohydrate Metabolism; Cluster Analysis; DNA, Bacterial / chemistry; DNA, Bacterial / genetics; DNA, Ribosomal / chemistry; DNA, Ribosomal / genetics; Electrophoresis, Gel, Two-Dimensional; Genetic Variation; Glycoside Hydrolases / genetics; Gram-Positive Bacteria / classification; Gram-Positive Bacteria / genetics; Gram-Positive Bacteria / growth & development; Gram-Positive Bacteria / metabolism; Hot Temperature; Microarray Analysis; Molecular Sequence Data; Nucleic Acid Hybridization; Phylogeny; Plants / microbiology; Proteome / analysis; RNA, Ribosomal, 16S / genetics; Sequence Analysis, DNA
TL;DR: Overall, the genus Caldicellulosiruptor appears to contain more genomic and physiological diversity than previously reported, and this argues for continued efforts to isolate new members from high-temperature terrestrial biotopes. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2010 journal article

Physiological Versatility of the Extremely Thermoacidophilic Archaeon Metallosphaera sedula Supported by Transcriptomic Analysis of Heterotrophic, Autotrophic, and Mixotrophic Growth

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 76(3), 931–935.

By: K. Auernik n & R. Kelly n

MeSH headings : Aerobiosis / genetics; Archaeal Proteins / genetics; Archaeal Proteins / metabolism; Autotrophic Processes; Carbon Dioxide / metabolism; Carbon Isotopes; Gene Expression Profiling; Gene Expression Regulation, Archaeal; Heterotrophic Processes; Hot Temperature; Hydroxybutyrates / metabolism; Oligonucleotide Array Sequence Analysis; Substrate Specificity / genetics; Sulfolobaceae / genetics; Sulfolobaceae / growth & development; Sulfolobaceae / metabolism; Temperature
TL;DR: Comparative transcriptomic analysis of autotroph, heterotrophic, and mixotrophic growth of the archaeon Metallosphaera sedula revealed candidates for yet-to-be-confirmed components of the 3-hydroxypropionate/4-Hydroxybutyrate pathway and implicated a membrane-bound hydrogenase for growth on H2. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2010 journal article

The genus Thermotoga: recent developments

ENVIRONMENTAL TECHNOLOGY, 31(10), 1169–1181.

By: A. Frock n, J. Notey n & R. Kelly n

author keywords: Thermotoga; extreme thermophiles; genomics; microbial physiology
MeSH headings : Biofilms; Carbohydrate Metabolism / genetics; Genome, Bacterial; Genomics; RNA, Ribosomal, 16S / genetics; Thermotoga maritima / enzymology; Thermotoga maritima / genetics; Thermotoga maritima / metabolism; Thermotoga maritima / physiology
TL;DR: Recent developments in genome sequences have become available for a number of Thermotoga species, leading to functional genomics efforts to understand growth physiology as well as genomics‐based identification and characterization of novel high‐temperature biocatalysts. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2009 journal article

Carbohydrate Utilization Patterns for the Extremely Thermophilic Bacterium Caldicellulosiruptor saccharolyticus Reveal Broad Growth Substrate Preferences

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 75(24), 7718–7724.

By: A. VanFossen n, M. Verhaart*, S. Kengen* & R. Kelly n

MeSH headings : ATP-Binding Cassette Transporters / genetics; ATP-Binding Cassette Transporters / metabolism; Biofuels; Biological Transport; Carbohydrate Metabolism; Gene Expression Profiling; Genes, Bacterial; Gram-Positive Bacteria / genetics; Gram-Positive Bacteria / growth & development; Lignin / metabolism; Monosaccharides / metabolism; Oligonucleotide Array Sequence Analysis
TL;DR: The results presented here affirm the broad growth substrate preferences of C. saccharolyticus on carbohydrates representative of lignocellulosic biomass and suggest that this bacterium holds promise for biofuel applications. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2009 journal article

Integrating modern biology into the ChE DNA through a campus-wide core laboratory education program

Chemical Engineering Education, 43(4), 257–264.

By: S. Carson, J. Chisnell & R. Kelly

Source: NC State University Libraries
Added: September 6, 2021

2009 journal article

Plant cell calcium-rich environment enhances thermostability of recombinantly produced α-amylase from the hyperthermophilic bacterium Thermotoga maritime

Biotechnology and Bioengineering, 104(5), 947–956.

By: M. Santa-Maria n, C. Chou n, G. Yencho n, C. Haigler n, W. Thompson n, R. Kelly n, B. Sosinski n

author keywords: hyperthermophilic enzymes; starch processing; transgenic plants; tobacco NT1 Cell cultures; biofuel
MeSH headings : Calcium / pharmacology; Coenzymes / pharmacology; Enzyme Stability; Escherichia coli / enzymology; Escherichia coli / genetics; Hot Temperature; Plants, Genetically Modified / enzymology; Recombinant Proteins / chemistry; Recombinant Proteins / genetics; Recombinant Proteins / metabolism; Thermotoga maritima / enzymology; Thermotoga maritima / genetics; Tobacco / enzymology; Tobacco / genetics; alpha-Amylases / chemistry; alpha-Amylases / genetics; alpha-Amylases / metabolism
TL;DR: In a proof of concept study, tobacco cell cultures were used as model system to test in planta production of a hyperthermophilic α‐amylase from Thermotoga maritima, demonstrating the importance of a calcium‐rich environment for thermostability, as well as the advantage of producing this enzyme directly in plant cells where calcium is readily available. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries, Crossref
Added: August 6, 2018

2009 journal article

Role of vapBC toxin–antitoxin loci in the thermal stress response of Sulfolobus solfataricus

Biochemical Society Transactions, 37(1), 123–126.

By: C. Cooper n, A. Daugherty*, S. Tachdjian n, P. Blum* & R. Kelly n

author keywords: archaeon; heat shock; hyperthermophile; stress response; Sulfolobus solfataricus; toxin-antioxin locus
MeSH headings : Antitoxins / metabolism; Archaeal Proteins / genetics; Archaeal Proteins / metabolism; Genome, Archaeal / genetics; Heat-Shock Response; Stress, Physiological; Sulfolobus solfataricus / genetics; Sulfolobus solfataricus / metabolism; Temperature; Toxins, Biological / genetics; Toxins, Biological / metabolism
TL;DR: Findings indicate that more work needs to be done to determine the role of VapBCs in S. solfataricus and other thermophilic archaea, especially with respect to post-transcriptional regulation. (via Semantic Scholar)
Sources: Crossref, NC State University Libraries
Added: August 28, 2020

2009 journal article

Temperature, not LuxS, mediates AI-2 formation in hydrothermal habitats

FEMS MICROBIOLOGY ECOLOGY, 68(2), 173–181.

By: J. Nichols n, M. Johnson n, C. Chou n & R. Kelly n

author keywords: autoinducer-2; AI-2; hyperthermophiles; hydrothermal environments; Thermotoga maritima; Pyrococcus furiosus
MeSH headings : Bacterial Proteins / genetics; Bacterial Proteins / metabolism; Carbon-Sulfur Lyases / genetics; Carbon-Sulfur Lyases / metabolism; Gas Chromatography-Mass Spectrometry; Gene Expression Regulation, Bacterial; Homoserine / analogs & derivatives; Homoserine / biosynthesis; Hot Temperature; Lactones; Pyrococcus furiosus / genetics; Pyrococcus furiosus / metabolism; Quorum Sensing; Thermotoga maritima / genetics; Thermotoga maritima / metabolism
TL;DR: Autoinducer-2 (AI-2), a boronated furanone and proposed 'universal' interspecies mesophilic bacterial communication signal, could be formed by Thermotoga maritima and Pyrococcus furiosus through a combination of biotic and abiotic reaction steps, but did not induce any detectable quorum-sensing phenotypes in these organisms. (via Semantic Scholar)
UN Sustainable Development Goal Categories
14. Life Below Water (OpenAlex)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2008 review

Extremely thermophilic microorganisms for biomass conversion: status and prospects

[Review of ]. CURRENT OPINION IN BIOTECHNOLOGY, 19(3), 210–217.

By: S. Blumer-Schuette n, I. Kataeva*, J. Westpheling*, M. Adams* & R. Kelly n

MeSH headings : Archaea / classification; Archaea / genetics; Archaea / metabolism; Bacteria / classification; Bacteria / genetics; Bacteria / metabolism; Bioelectric Energy Sources; Biomass; Biotechnology / trends; Carbohydrate Metabolism; Cellulase / metabolism; Cellulose / metabolism; Energy-Generating Resources; Hot Temperature; Marine Biology; Phylogeny
TL;DR: Characterization of extremely thermophilic enzymes implicated in the deconstruction of lignocellulose can be identified in genome sequences, and many more promising biocatalysts probably remain annotated as 'hypothetical proteins'. (via Semantic Scholar)
UN Sustainable Development Goal Categories
14. Life Below Water (OpenAlex)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2008 chapter

Functional Genomics of Stress Response in Extremophilic Archaea

In P. Blum (Ed.), Archaea: New Models for Prokaryotic Biology. Norfolk, UK: Caister Academic Press.

By: S. Tachdjian, K. Shockley, S. Conners & R. Kelly

Ed(s): P. Blum

Source: NC State University Libraries
Added: September 9, 2021

2008 journal article

Functional-genomics-based identification and characterization of open reading frames encoding alpha-glucoside-processing enzymes in the hyperthermophilic archaeon Pyrococcus furiosus

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 74(4), 1281–1283.

By: D. Comfort n, C. Chou n, S. Conners n, A. VanFossen n & R. Kelly n

MeSH headings : Computational Biology; Gene Expression Regulation, Bacterial / genetics; Genomics / methods; Open Reading Frames / genetics; Pyrococcus furiosus / enzymology; Pyrococcus furiosus / genetics; alpha-Glucosidases / genetics; beta-Amylase / genetics
TL;DR: Bioinformatics analysis and transcriptional response information for Pyrococcus furiosus led to the identification of a novel isomaltase representing a new glycoside hydrolase family, a novel GH57 β-amylase, and an extracellular starch-binding protein. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2008 review

Hydrogenesis in hyperthermophilic microorganisms: Implications for biofuels

[Review of ]. METABOLIC ENGINEERING, 10(6), 394–404.

By: C. Chou n, F. Jenney*, M. Adams* & R. Kelly n

author keywords: Biohydrogen; Pyrococcus furiosus; Thermotoga maritima; Caldicellulosiruptor saccharolyticus; Hyperthermophiles; Hydrogenases
MeSH headings : Archaea / metabolism; Bioelectric Energy Sources; Hot Temperature; Hydrogen / metabolism; Hydrogen Sulfide / metabolism; Methane / metabolism
TL;DR: To fully exploit the use of these novel microorganisms and their constituent hydrogenases for biohydrogen production, development of versatile genetic systems and improvements in current understanding of electron flux from fermentable substrates to H(2) in hyperthermophiles are needed. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2008 journal article

Hydrogenomics of the Extremely Thermophilic Bacterium Caldicellulosiruptor saccharolyticus

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 74(21), 6720–6729.

By: H. Werken*, M. Verhaart*, A. VanFossen n, K. Willquist*, D. Lewis n, J. Nichols n, H. Goorissen*, E. Mongodin* ...

MeSH headings : Bacterial Proteins / genetics; Carbohydrate Metabolism / genetics; DNA, Bacterial / chemistry; DNA, Bacterial / genetics; Enzymes / genetics; Gene Expression Profiling; Genome, Bacterial; Gram-Positive Bacteria / genetics; Metabolic Networks and Pathways / genetics; Molecular Sequence Data; Sequence Analysis, DNA
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2008 journal article

Identification of Components of Electron Transport Chains in the Extremely Thermoacidophilic Crenarchaeon Metallosphaera sedula through Iron and Sulfur Compound Oxidation Transcriptomes

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 74(24), 7723–7732.

By: K. Auernik n & R. Kelly n

MeSH headings : Archaeal Proteins / biosynthesis; Archaeal Proteins / genetics; Electron Transport Chain Complex Proteins / biosynthesis; Electron Transport Chain Complex Proteins / genetics; Ferrous Compounds / metabolism; Gene Expression Profiling; Genes, Archaeal; Iron-Sulfur Proteins / biosynthesis; Iron-Sulfur Proteins / genetics; Multigene Family; Oxidation-Reduction; Oxidoreductases / biosynthesis; Oxidoreductases / genetics; Sulfolobaceae / enzymology; Sulfolobaceae / genetics; Sulfolobaceae / metabolism; Sulfur / metabolism
TL;DR: From this analysis, a comprehensive model for electron transport in M. sedula could be proposed as the basis for examining specific details of iron and sulfur oxidation in this bioleaching archaeon. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2008 review

Life in hot acid: Pathway analyses in extremely thermoacidophilic archaea

[Review of ]. CURRENT OPINION IN BIOTECHNOLOGY, 19(5), 445–453.

By: K. Auernik n, C. Cooper n & R. Kelly n

MeSH headings : Acids / metabolism; Archaea / physiology; Archaeal Proteins / metabolism; Cell Survival; Ecosystem; Hot Temperature; Hydrogen-Ion Concentration; Models, Biological; Signal Transduction / physiology
TL;DR: New insights into the metabolic pathways and physiological features that define extreme thermoacidophily have been obtained, in some cases suggesting prospects for biotechnological opportunities. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2008 journal article

Microwave activation of enzymatic catalysis

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 130(31), 10048-+.

By: D. Young n, J. Nichols n,