Works (338)
Updated: April 3rd, 2024 19:55
2024 article
Complete genome sequence for the extremely thermophilic bacterium Anaerocellum danielii (DSM:8977)
Manesh, M. J. H., Bing, R. G., Willard, D. J., Adams, M. W. W., & Kelly, R. M. (2024, January 24). (K. M. Stedman, Ed.). MICROBIOLOGY RESOURCE ANNOUNCEMENTS, Vol. 1.
Ed(s): K. Stedman
author keywords: Anaerocellum danielii; extreme thermophile; cellulose
TL;DR:
The complete genome sequence of the extremely thermophilic bacterium Anaerocellum Anaerocellum danielii (DSM:8977) is reported here and is capable of lignocellulose degradation with potential applications in biomass degradation and production of chemicals and fuels from renewable feedstocks.
(via Semantic Scholar)
doi.org (publisher)
Sources: Web Of Science, NC State University Libraries, ORCID
Added: February 12, 2024
2024 article
Complete genome sequence for the thermoacidophilic archaeon <i>Metallosphaera sedula</i> (DSM:5348)
Manesh, M. J. H., Bing, R. G., Willard, D. J., & Kelly, R. M. (2024, February 8). MICROBIOLOGY RESOURCE ANNOUNCEMENTS, Vol. 2.
author keywords: Metallosphaera sedula; thermoacidophile; archeaon
TL;DR:
The complete genome sequence of the thermoacidophilic archaeon Metallosphaera sedula, originally isolated from a volcanic field in Italy, is reported here, and has applications in bioleaching of sulfide minerals.
(via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: February 19, 2024
2024 review
Extremely thermoacidophilic archaea for metal bioleaching:<i> What</i><i> do</i><i> their</i> genomes tell Us?
[Review of ]. BIORESOURCE TECHNOLOGY, 391.
author keywords: Archaea; Sulfolobales; Metal biooxidation; Sulfur biooxidation; Chalcopyrite; High temperatures
TL;DR:
The evolving landscape for bioleaching technologies at high temperatures merits a closer look through a genomic lens at what is currently possible and what lies ahead in terms of new developments and emerging opportunities.
(via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: December 18, 2023
2023 article
Complete Genome Sequences of Caldicellulosiruptor acetigenus DSM 7040, Caldicellulosiruptor morganii DSM 8990 (RT8.B8), and Caldicellulosiruptor naganoensis DSM 8991 (NA10)
Bing, R. G. G., Willard, D. J. J., Manesh, M. J. H., Laemthong, T., Crosby, J. R. R., Adams, M. W. W., & Kelly, R. M. M. (2023, February 1). MICROBIOLOGY RESOURCE ANNOUNCEMENTS, Vol. 2.
TL;DR:
The genome sequences of three extremely thermophilic, lignocellulolytic Caldicellulosiruptor species were closed, improving previously reported multiple-contig assemblies and enhancing bioinformatic analysis of the species.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: February 20, 2023
2023 journal article
Complete Genome Sequences of Two Thermophilic Indigenous Bacteria Isolated from Wheat Straw, Thermoclostridium stercorarium subsp. Strain RKWS1 and Thermoanaerobacter sp. Strain RKWS2
MICROBIOLOGY RESOURCE ANNOUNCEMENTS, 12(3).
TL;DR:
Discovery of indigenous thermophiles in plant biomass suggests that high-temperature microorganisms are more ubiquitous than previously thought.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: April 17, 2023
2023 article
Complete genome sequence for the thermoacidophilic archaeon <i>Sulfuracidifex</i> (f<i>. Sulfolobus</i>) <i>metallicus</i> DSM 6482
Willard, D. J., Manesh, M. J. H., Bing, R. G., & Kelly, R. M. (2023, December 6). MICROBIOLOGY RESOURCE ANNOUNCEMENTS, Vol. 12.
author keywords: thermoacidophile; archaea; crenarchaea; genome
TL;DR:
The complete genome sequence for the thermoacidophilic archaeon Sulfuracidifex (f. Sulfolobus) metallicus DSM 6482 is reported, which is a prolific metal and sulfur oxidizer and has application in metal bioleaching operations.
(via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: December 18, 2023
2023 journal article
Fermentative conversion of unpretreated plant biomass: A thermophilic threshold for indigenous microbial growth
BIORESOURCE TECHNOLOGY, 367.
author keywords: Thermophiles; Lignocellulose; Consolidated bioprocessing; Caldicellulosiruptor bescii; Acetivibrio thermocellus
MeSH headings : Biomass; Fermentation; Lignin / chemistry; Clostridium thermocellum / metabolism; Plants / metabolism
TL;DR:
Thermophiles that naturally grow at and above 75°C offer specific advantages as platform microorganisms for biomass conversion into fuels and chemicals that prevents native microbes from competing with engineered microbes and forming undesirable by-products.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
7. Affordable and Clean Energy
(Web of Science)
12. Responsible Consumption and Production
(Web of Science)
Sources: Web Of Science, NC State University Libraries, ORCID
Added: January 17, 2023
2023 article
Innovating Life Sciences Laboratory Training: Molecular Biology Laboratory Education Modules (MBLEMs) as a Model for Advanced Training at Diverse Institutions
JOURNAL OF BIOLOGICAL CHEMISTRY, Vol. 299, pp. S260–S260.
Sources: Web Of Science, NC State University Libraries
Added: September 11, 2023
2023 article
Interplay between transcriptional regulators and VapBC toxin-antitoxin loci during thermal stress response in extremely thermoacidophilic archaea
Cooper, C. R., Lewis, A. M., Notey, J. S., Mukherjee, A., Willard, D. J., Blum, P. H., & Kelly, R. M. (2023, February 24). ENVIRONMENTAL MICROBIOLOGY, Vol. 2.
MeSH headings : Antitoxins / genetics; Toxins, Biological / genetics; Toxins, Biological / metabolism; Heat-Shock Response / genetics; Sulfolobus solfataricus / genetics; Sulfolobus solfataricus / metabolism; Escherichia coli / genetics
TL;DR:
The interplay between VapBC TA loci and heat shock regulators in Sa.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: March 13, 2023
2023 article
Manipulating Fermentation Pathways in the Hyperthermophilic Archaeon Pyrococcus furiosus for Ethanol Production up to 95 degrees C Driven by Carbon Monoxide Oxidation
Lipscomb, G. L., Crowley, A. T., Nguyen, D. M. N., Keller, M. W., Hailey C. O'Quinn, Tanwee, T. N. N., … Adams, M. W. W. (2023, May 10). APPLIED AND ENVIRONMENTAL MICROBIOLOGY, Vol. 5.
author keywords: Pyrococcus furiosus; archaea; thermophile; ethanol; genetic engineering; CO dehydrogenase; bioethanol; carbon monoxide; hyperthermophiles; metabolic engineering; metabolic modeling
TL;DR:
An engineered strain of P. furiosus is described that synthesizes ethanol at 95°C, utilizing a homologously-expressed native alcohol dehydrogenase, termed AdhF, and opens the door to the potential for ‘bioreactive distillation’ since fermentation can be performed well above the normal boiling point of ethanol.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
7. Affordable and Clean Energy
(Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: May 22, 2023
2023 article
Metabolic engineering of <i>Caldicellulosiruptor bescii</i> for 2,3-butanediol production from unpretreated lignocellulosic biomass and metabolic strategies for improving yields and titers
Tanwee, T. N. N., Lipscomb, G. L., Vailionis, J. L., Zhang, K., Bing, R. G., Hailey C. O'Quinn, … Adams, M. W. W. (2023, December 22). APPLIED AND ENVIRONMENTAL MICROBIOLOGY, Vol. 12.
author keywords: Caldicellulosiruptor bescii; thermophile; 2,3-butanediol; acetoin; lignocellulose; metabolic flux; redox balance
Sources: Web Of Science, NC State University Libraries
Added: January 8, 2024
2023 journal article
Multiplex CRISPR editing of wood for sustainable fiber production
SCIENCE, 381(6654), 216-+.
TL;DR:
This work demonstrates that genome editing can be harnessed for breeding more efficient trees, which will provide timely opportunities for sustainable forestry and a more efficient bioeconomy.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, NC State University Libraries, ORCID
Added: August 9, 2023
2023 article
Optimizing Strategies for Bio-Based Ethanol Production Using Genome-Scale Metabolic Modeling of the Hyperthermophilic Archaeon, Pyrococcus furiosus
Vailionis, J. L., Zhao, W., Zhang, K., Rodionov, D. A., Lipscomb, G. L., Tanwee, T. N. N., … Zhang, Y. (2023, June 8). APPLIED AND ENVIRONMENTAL MICROBIOLOGY, Vol. 6.
author keywords: Pyrococcus furiosus; thermophiles; ethanol; genome-scale modeling; CO dehydrogenase; Archaea
TL;DR:
The P. furiosus model is a powerful tool for understanding the relationship between generation of end products and redox/energy balance at a systems-level that will aid in the design of optimal engineering strategies for production of bio-based chemicals and fuels.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
7. Affordable and Clean Energy
(Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: June 19, 2023
2023 journal article
Role of cell-substrate association during plant biomass solubilization by the extreme thermophile Caldicellulosiruptor bescii
EXTREMOPHILES, 27(1).
author keywords: Caldicellulosiruptor; Extreme thermophile; Lignocellulose; Binding proteins; Tapirins
MeSH headings : Cellulose / metabolism; Biomass; Saccharum / metabolism; Caldicellulosiruptor / metabolism; Clostridiales / metabolism; Plants; Archaea / metabolism
TL;DR:
Results show that enhanced binding to the substrate, beyond the native capability, did not improve C. bescii solubilization of plant biomass, but in some cases may improve conversion of released lignocellulose carbohydrates to fermentation products.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
7. Affordable and Clean Energy
(Web of Science)
12. Responsible Consumption and Production
(Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: March 13, 2023
2023 article
Stay or Go: Sulfolobales Biofilm Dispersal Is Dependent on a Bifunctional VapB Antitoxin
Lewis, A. M., Willard, D. J., Manesh, M. J. H. J., Sivabalasarma, S., Albers, S.-V., & Kelly, R. M. (2023, April 10). MBIO, Vol. 4.
author keywords: toxin-antitoxin loci; biofilms; Sulfolobales; thermoacidophiles; Sulfolobus acidocaldarius
MeSH headings : Antitoxins / metabolism; Bacterial Proteins / metabolism; Bacterial Toxins / metabolism; Sulfolobales; Biofilms
TL;DR:
The findings here suggest that a stand-alone VapB-type antitoxin was the product of selective evolutionary pressure to influence biofilm formation in these archaea, a vital microbial community behavior.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: May 9, 2023
2023 journal article
Whither the genus Caldicellulosiruptor and the order Thermoanaerobacterales: phylogeny, taxonomy, ecology, and phenotype
FRONTIERS IN MICROBIOLOGY, 14.
author keywords: Caldicellulosiruptor; Thermoanaerobacterales; bacteria; thermophiles; phylogeny; ecology; fermentative anaerobes
TL;DR:
A main outcome of ANI/AAI analysis indicates the need to reclassify several previously designated species in the Thermoanaerobacterales and Caldicellulosiruptorales by condensing them into strains of single species.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
7. Affordable and Clean Energy
(Web of Science)
12. Responsible Consumption and Production
(Web of Science)
14. Life Below Water
(OpenAlex)
Sources: Web Of Science, NC State University Libraries
Added: August 28, 2023
2022 journal article
<p>Plant biomass fermentation by the extreme thermophile Caldicellulosiruptor bescii for co-production of green hydrogen and acetone: Technoeconomic analysis</p>
BIORESOURCE TECHNOLOGY, 348.
author keywords: Acetone; Green hydrogen; Caldicellulosiruptor bescii; Extreme thermophiles; Technoeconomic analysis
MeSH headings : Acetone; Biomass; Caldicellulosiruptor; Fermentation; Hydrogen; Lignin / chemistry
UN Sustainable Development Goal Categories
7. Affordable and Clean Energy
(Web of Science)
12. Responsible Consumption and Production
(Web of Science)
Sources: Web Of Science, NC State University Libraries, ORCID
Added: April 25, 2022
2022 article
Biochemical and Regulatory Analyses of Xylanolytic Regulons in Caldicellulosiruptor bescii Reveal Genus-Wide Features of Hemicellulose Utilization
Crosby, J. R., Laemthong, T., Bing, R. G., Zhang, K., Tanwee, T. N. N., Lipscomb, G. L., … Kelly, R. M. (2022, October 11). APPLIED AND ENVIRONMENTAL MICROBIOLOGY, Vol. 10.
author keywords: Caldicellulosiruptor; extreme thermophile; hemicellulose; xylanases; hemicellulase
MeSH headings : Regulon; Xylans; Cellulose / metabolism; Clostridiales / metabolism; Sugars
TL;DR:
Overall, this work demonstrates the essential requirements for Caldicellulosiruptor to degrade various forms of xylan and the differences in species genomic inventories that are tuned for survival in unique biotopes with variable lignocellulosic substrates.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
7. Affordable and Clean Energy
(Web of Science)
12. Responsible Consumption and Production
(Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: October 24, 2022
2022 article
Engineering Caldicellulosiruptor bescii with Surface Layer Homology Domain-Linked Glycoside Hydrolases Improves Plant Biomass Solubilization
Laemthong, T., Bing, R. G., Crosby, J. R., Adams, M. W. W., & Kelly, R. M. (2022, September 28). APPLIED AND ENVIRONMENTAL MICROBIOLOGY, Vol. 9.
author keywords: Caldicellulosiruptor; lignocellulose; glycoside hydrolase; surface layer homology (SLH) domain; plant biomass degradation; surface layer homology domain
MeSH headings : Glycoside Hydrolases / metabolism; Biomass; Xylans / metabolism; Xylose; Clostridiales / metabolism; Cellulose / metabolism; Plants / microbiology; Populus
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: October 11, 2022
2021 journal article
A genomic catalog of Earth’s microbiomes
Nature Biotechnology, 39, 499–509.
MeSH headings : Air Microbiology; Animals; Archaea / classification; Archaea / genetics; Archaea / isolation & purification; Bacteria / classification; Bacteria / genetics; Bacteria / isolation & purification; Catalogs as Topic; Ecosystem; Humans; Metabolomics / methods; Metagenome; Metagenomics / methods; Phylogeny; Soil Microbiology; Viruses / genetics; Viruses / isolation & purification; Water Microbiology
TL;DR:
The utility of this collection of >10,000 metagenomes collected from diverse habitats covering all of Earth’s continents and oceans is demonstrated for understanding secondary-metabolite biosynthetic potential and for resolving thousands of new host linkages to uncultivated viruses.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
14. Life Below Water
(OpenAlex)
Sources: Web Of Science, NC State University Libraries
Added: July 27, 2021
2021 chapter
Enzymes from extremely thermophilic bacteria and archaea: Current status and future prospects
In N. A. Arora, S. Agnihotri, & J. Mishra (Eds.), Extremozymes and their Industrial Applications. St. Louis, MO: Elsevier Academic Press.
Ed(s): N. Arora, S. Agnihotri & J. Mishra
Source: NC State University Libraries
Added: July 27, 2021
2021 article
Fox Cluster determinants for iron biooxidation in the extremely thermoacidophilic Sulfolobaceae
Counts, J. A., Vitko, N. P., & Kelly, R. M. (2021, August 30). ENVIRONMENTAL MICROBIOLOGY, Vol. 8.
MeSH headings : Animals; Archaeal Proteins / genetics; Archaeal Proteins / metabolism; Iron / metabolism; Phylogeny; Proteomics; Sulfolobaceae / genetics
TL;DR:
Proteomic analysis here of iron-oxidizing membranes from M. sedula indicates that FoxA2 and FoxB and FoxC are essential and few homologs exist for FoxC or for most other Fox Cluster proteins.
(via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: September 7, 2021
2021 journal article
Genome-Scale Metabolic Model of Caldicellulosiruptor bescii Reveals Optimal Metabolic Engineering Strategies for Bio-based Chemical Production
MSystems, 6(3).
Ed(s): Z. Summers
author keywords: Caldicellulosiruptor bescii; lignocellulose degradation; carbohydrate; Caldicellulosiruptor; carbohydrate utilization; comparative genomics; metabolic reconstruction; plant biomass degradation; regulon
TL;DR:
The C. bescii model provides a useful platform for investigating the potential redox controls that mediate the carbon and energy flows in metabolism and sets the stage for future design of engineering strategies aiming at optimizing the production of ethanol and other bio-based chemicals.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
7. Affordable and Clean Energy
(Web of Science)
12. Responsible Consumption and Production
(Web of Science)
Sources: Crossref, NC State University Libraries, Web Of Science
Added: July 14, 2021
2021 journal article
Integrating Bioinformatics Tools into Inquiry-based Molecular Biology Laboratory Education Modules
Frontiers in Education, 6.
author keywords: bioinformatics; science education; molecular biotechnology; software tools; case studies
TL;DR:
This new paradigm for biology laboratory education that embraces bioinformatics as a critical component of inquiry-based learning for undergraduate and graduate students representing the life sciences, the physical sciences, and engineering is described.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: August 23, 2021
2021 journal article
Intersection of Biotic and Abiotic Sulfur Chemistry Supporting Extreme Microbial Life in Hot Acid
JOURNAL OF PHYSICAL CHEMISTRY B, 125(20), 5243–5257.
MeSH headings : Metals; Oxidation-Reduction; Oxidoreductases / metabolism; Sulfolobus acidocaldarius; Sulfur
TL;DR:
Extreme thermoacidophily, a characteristic of species in the archaeal order Sulfolobales, is considered from the perspective of sulfur chemistry, both biotic and abiotic, as it relates to microbial bioenergetics.
(via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: June 14, 2021
2021 journal article
Intersection of Biotic and Abiotic Sulfur Chemistry Supporting Extreme Microbial Life in Hot Acid
Journal of Physical Chemistry B, 125(20), 5243–5257.
Sources: Crossref, NC State University Libraries
Added: July 27, 2021
2021 journal article
The biology of thermoacidophilic archaea from the order Sulfolobales
FEMS Microbiology Reviews, 45(4).
author keywords: Archaea; Thermoacidophiles; Sulfolobales
MeSH headings : Archaea / genetics; Biology; Iron; Sulfolobales
TL;DR:
The nuances of the thermoacidophilic lifestyle of the Sulfolobales are discussed, including their evolutionary placement, cell biology, survival strategies, genetic tools, metabolic processes, and physiological attributes together with how these characteristics make thermo Acidophiles ideal platforms for specialized industrial processes.
(via Semantic Scholar)
Sources: Crossref, NC State University Libraries, Web Of Science
Added: July 14, 2021
2021 journal article
Thermophilic microbial deconstruction and conversion of natural and transgenic lignocellulose
Environmental Microbiology Reports, 13(3), 272–293.
MeSH headings : Biomass; Lignin / chemistry; Plants / metabolism
TL;DR:
Bioengineering plant properties to better fit the deconstruction capabilities of candidate consolidated bioprocessing microorganisms has potential to achieve the efficient lignocellulose deconstruction needed for industrial relevance.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
7. Affordable and Clean Energy
(Web of Science)
12. Responsible Consumption and Production
(Web of Science)
Sources: Web Of Science, Crossref, NC State University Libraries, ORCID
Added: April 12, 2021
2021 journal article
Transcriptional Regulation of Plant Biomass Degradation and Carbohydrate Utilization Genes in the Extreme Thermophile Caldicellulosiruptor bescii
MSystems, 6(3).
Ed(s): Z. Summers
TL;DR:
The mechanisms of transcriptional regulation of carbohydrate utilization genes using a comparative genomics approach applied to 14 Caldicellulosiruptor species were investigated and it was confirmed that the shared ATPase component MsmK is essential for growth on oligo- and polysaccharides but not for the utilization of monosaccharides.
(via Semantic Scholar)
Sources: Crossref, NC State University Libraries
Added: July 14, 2021
2020 journal article
Engineering the cellulolytic extreme thermophile Caldicellulosiruptor bescii to reduce carboxylic acids to alcohols using plant biomass as the energy source
Journal of Industrial Microbiology and Biotechnology, 47(8), 585–597.
author keywords: Thermophile; Biofuels; Lignocellulose; Metabolic engineering; Caldicellulosiruptor
MeSH headings : Alcohol Dehydrogenase / genetics; Alcohol Dehydrogenase / metabolism; Aldehyde Oxidoreductases / genetics; Aldehyde Oxidoreductases / metabolism; Biofuels / analysis; Biomass; Caldicellulosiruptor / genetics; Carboxylic Acids / metabolism; Ethanol / metabolism; Fermentation; Lignin / metabolism; Microorganisms, Genetically-Modified; Oxidation-Reduction; Panicum / metabolism; Panicum / microbiology; Pyrococcus furiosus / enzymology; Thermoanaerobacter / enzymology
TL;DR:
This work investigates the ability of engineered C.bescii to generate alcohols from carboxylic acids and converts them to alcohol using both crystalline cellulose and switchgrass as sources of reductant for alcohol production, the first instance of an acid to alcohol conversion pathway in a cellulolytic microbe.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
7. Affordable and Clean Energy
(Web of Science; OpenAlex)
12. Responsible Consumption and Production
(Web of Science)
Sources: Web Of Science, NC State University Libraries, Crossref
Added: August 31, 2020
2020 journal article
Genome Sequences of Five Type Strain Members of the Archaeal Family Sulfolobaceae, Acidianus ambivalens, Acidianus infernus, Stygiolobus azoricus, Sulfuracidifex metallicus, and Sulfurisphaera ohwakuensis
MICROBIOLOGY RESOURCE ANNOUNCEMENTS, 9(11).
TL;DR:
Five genomes from the polyextremophilic (optimal temperature of >65°C and optimal pH of <3.5) archaeal family Sulfolobaceae are presented, greatly expanding order-wide genomic diversity.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
15. Life on Land
(Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: April 6, 2020
2020 journal article
Life in hot acid: a genome‐based reassessment of the archaeal order Sulfolobales
Environmental Microbiology, 23(7), 3568–3584.
MeSH headings : Acidianus; Archaea / genetics; Hot Springs; Oxidation-Reduction; Phylogeny; Sulfolobaceae / genetics; Sulfolobales
TL;DR:
Despite the presence of facultative anaerobes and one confirmed obligate anaerobe, oxidase complexes are prevalent in many species, suggesting a key role for oxygen among the Sulfolobales.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
15. Life on Land
(Web of Science)
Sources: Web Of Science, Crossref, NC State University Libraries
Added: September 21, 2020
2020 journal article
Metabolically engineeredCaldicellulosiruptor besciias a platform for producing acetone and hydrogen from lignocellulose
BIOTECHNOLOGY AND BIOENGINEERING, 117(12), 3799–3808.
author keywords: acetone; Caldicellulosiruptor bescii; extreme thermophiles; metabolic engineering
MeSH headings : Acetone / metabolism; Biomass; Caldicellulosiruptor / genetics; Caldicellulosiruptor / metabolism; Hydrogen / metabolism; Lignin / metabolism; Metabolic Engineering
TL;DR:
C. bescii can be engineered with balanced pathways in which renewable carbohydrate sources are converted to useful metabolites, primarily acetone and H2, without net production of its native fermentation products, acetate and lactate.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
12. Responsible Consumption and Production
(Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: September 7, 2020
2020 journal article
Modification of the glycolytic pathway in Pyrococcus furiosus and the implications for metabolic engineering
EXTREMOPHILES, 24(4), 511–518.
author keywords: Pyrococcus; Thermophile; Central metabolism; Glycolysis
MeSH headings : Fermentation; Glyceraldehyde 3-Phosphate; Glycolysis; Metabolic Engineering; Pyrococcus furiosus
TL;DR:
Insertion of a single gene encoding the thermostable NADPH-dependent primary alcohol dehydrogenase (adhA) from Caldanaerobacter subterraneus resulted in a strain producing ethanol via the previously established aldehyde oxidoreductase (AOR) pathway, and demonstrated a high ratio of ethanol over acetate at 80 °C and enabled ethanol production up to 85 °C, the highest temperature for bio-ethanol production reported to date.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
7. Affordable and Clean Energy
(Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: June 1, 2020
2020 article
The biology and biotechnology of the genus Caldicellulosiruptor: recent developments in 'Caldi World'
Lee, L. L., Crosby, J. R., Rubinstein, G. M., Laemthong, T., Bing, R. G., Straub, C. T., … Kelly, R. M. (2020, January). EXTREMOPHILES, Vol. 24, pp. 1–15.
author keywords: Caldicellulosiruptor; Extreme thermophiles; Lignocellulose
MeSH headings : Biomass; Biotechnology; Clostridiales; Glycoside Hydrolases; Hot Springs
TL;DR:
Improvements in the amount of carbohydrate released and in the production of bio-based chemicals are required to successfully realize the biotechnological potential of Caldicellulosiruptor organisms.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
7. Affordable and Clean Energy
(Web of Science)
12. Responsible Consumption and Production
(Web of Science)
Sources: Web Of Science, NC State University Libraries, ORCID
Added: February 27, 2020
2020 journal article
Use of the lignocellulose-degrading bacterium Caldicellulosiruptor bescii to assess recalcitrance and conversion of wild-type and transgenic poplar
BIOTECHNOLOGY FOR BIOFUELS, 13(1).
author keywords: Caldicellulosiruptor; Extreme thermophiles; Lignocellulose; Biofuel; Poplar
TL;DR:
Utilizing C. bescii as a screening assay for recalcitrance, poplar lines with down-regulation of coumarate 3-hydroxylase 3 (C3H3) resulted in the highest degrees of solubilization and conversion by C. Bescii, suggesting that excellent digestibility can be achieved without compromising fitness of the tree.
(via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries, ORCID
Added: April 6, 2020
2019 journal article
Comparative Biochemical and Structural Analysis of Novel Cellulose Binding Proteins (Tapirins) from Extremely Thermophilic Caldicellulosiruptor Species
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 85(3).
author keywords: Caldicellulosiruptor; cellulose binding protein; lignocellulose; tapirin
MeSH headings : Amino Acid Sequence; Bacterial Proteins / chemistry; Bacterial Proteins / genetics; Bacterial Proteins / metabolism; Carrier Proteins / chemistry; Carrier Proteins / genetics; Carrier Proteins / metabolism; Cellulose / chemistry; Cellulose / metabolism; Firmicutes / chemistry; Firmicutes / genetics; Firmicutes / metabolism; Genome, Bacterial; Hot Springs / microbiology; Hot Temperature; Protein Domains
TL;DR:
Densitometry analysis of bound protein fractions visualized by SDS-PAGE revealed that Calhy_0908 and Calkr_0826 (from weakly cellulolytic species) associated with the cellulose substrates to a greater extent than Athe_1870, Calkro_0844, and NA10_0869 (from strongly cellulolyic species).
(via Semantic Scholar)
UN Sustainable Development Goal Categories
7. Affordable and Clean Energy
(Web of Science)
12. Responsible Consumption and Production
(Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: February 11, 2019
2019 journal article
Determinants of sulphur chemolithoautotrophy in the extremely thermoacidophilic Sulfolobales
ENVIRONMENTAL MICROBIOLOGY, 21(10), 3696–3710.
MeSH headings : Autotrophic Processes; Chemoautotrophic Growth; Oxidation-Reduction; Oxidoreductases / metabolism; Sulfolobales / metabolism; Sulfur / metabolism; Thiosulfates / metabolism
TL;DR:
Comparative transcriptomic analyses of Acidianus brierleyi, a chemolithoautotrophic sulfur oxidizer, and S. acidocaldarius DSM639 showed that while both share a strong transcriptional response to elemental sulfur, both failed to up-regulate key 3-HP/4-HB cycle genes used by A. briersleyi to drive chemolithOautotrophy.
(via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: August 12, 2019
2019 journal article
Extreme thermophiles as emerging metabolic engineering platforms
Current Opinion in Biotechnology, 59, 55–64.
MeSH headings : Archaea; Biotechnology; Genetic Engineering; Metabolic Engineering; Waste Products
TL;DR:
If non-model platforms are successfully deployed, new avenues for production of fuels and chemicals from renewable feedstocks or waste materials will emerge.
(via Semantic Scholar)
Sources: Web Of Science, Crossref, NC State University Libraries
Added: November 25, 2019
2019 journal article
Extremely Thermoacidophilic Metallosphaera Species Mediate Mobilization and Oxidation of Vanadium and Molybdenum Oxides
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 85(5).
author keywords: Metallosphaera; biomining; extreme thermoacidophily; metal biooxidation; molybdenum; vanadium
MeSH headings : Archaeal Proteins / genetics; Copper / metabolism; Ferric Compounds / metabolism; Gene Expression Profiling; Genome, Archaeal; Hot Temperature; Hydrogen-Ion Concentration; Molybdenum / metabolism; Oxidation-Reduction; Oxides / metabolism; Oxygen; Sulfolobaceae / genetics; Sulfolobaceae / metabolism; Sulfur Compounds / metabolism; Transcriptome; Uranium / metabolism; Vanadium / metabolism
TL;DR:
Results suggest that direct oxidation of V and Mo occurs, in addition to Fe-mediated oxidation, such that both direct and indirect mechanisms are involved in the mobilization of redox-active metals by Metallosphaera species.
(via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: March 11, 2019
2019 journal article
Lignocellulose solubilization and conversion by extremely thermophilic Caldicellulosiruptor bescii improves by maintaining metabolic activity
BIOTECHNOLOGY AND BIOENGINEERING, 116(8), 1901–1908.
author keywords: biofuels; bioreactor; Caldicellulosiruptor bescii; lignocellulose; switchgrass
MeSH headings : Biofuels / microbiology; Bioreactors; Caldicellulosiruptor; Fermentation; Firmicutes / growth & development; Firmicutes / metabolism; Lignin / metabolism; Panicum / metabolism; Solubility
TL;DR:
The extreme thermophile Caldicellulosiruptor bescii solubilized carbohydrate conversion to fermentation products increased from 40% in batch to over 80% in the purged system, an improvement attributed to maintaining the bioreactor culture in a metabolically active state.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
7. Affordable and Clean Energy
(Web of Science)
12. Responsible Consumption and Production
(Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: August 19, 2019
2019 journal article
Quantitative fermentation of unpretreated transgenic poplar by Caldicellulosiruptor bescii
NATURE COMMUNICATIONS, 10(1).
Contributors: C. Straub n, P. Khatibi n, J. Wang n , J. Conway n, A. Williams-Rhaesa*, I. Peszlen n , V. Chiang n, M. Adams *, n
MeSH headings : Biomass; Cellulose / metabolism; Clostridiales / genetics; Clostridiales / metabolism; Ethanol / metabolism; Fermentation; Industrial Microbiology; Lignin / metabolism; Metabolic Engineering; Plants, Genetically Modified / chemistry; Plants, Genetically Modified / genetics; Plants, Genetically Modified / metabolism; Polysaccharides / metabolism; Populus / chemistry; Populus / genetics; Populus / metabolism
TL;DR:
The authors pair transgenic lignin modified poplar lines with engineered Caldicellusiruptor bescii to achieve biomass solubilization and ethanol conversion without pretreatment and the nearly quantitative microbial conversion of the carbohydrate content of unpretreated transgen lignocellulosic biomass bodes well for full utilization of renewable biomass feedstocks.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
7. Affordable and Clean Energy
(Web of Science)
12. Responsible Consumption and Production
(Web of Science)
Sources: Web Of Science, NC State University Libraries, ORCID
Added: August 26, 2019
2019 journal article
The thermophilic biomass-degrading bacterium Caldicellulosiruptor bescii utilizes two enzymes to oxidize glyceraldehyde 3-phosphate during glycolysis
JOURNAL OF BIOLOGICAL CHEMISTRY, 294(25), 9995–10005.
author keywords: glycolysis; cellulose; metabolism; metabolomics; metalloenzyme; oxidation-reduction (redox); anaerobic glycolysis; iron-sulfur protein; pterin; tungsten
MeSH headings : Biomass; Caldicellulosiruptor; Firmicutes / growth & development; Firmicutes / metabolism; Glyceraldehyde 3-Phosphate / chemistry; Glyceraldehyde 3-Phosphate / metabolism; Glyceraldehyde 3-Phosphate Dehydrogenase (NADP+) / metabolism; Glyceraldehyde-3-Phosphate Dehydrogenases / metabolism; Glycolysis; Metabolome; Oxidation-Reduction; Phylogeny
TL;DR:
Using metabolomics and kinetic analyses, it is shown that GOR functions in parallel with the conventional GAP dehydrogenase, providing an alternative ferredoxin-dependent glycolytic pathway and likely facilitates the recycling of reduced redox carriers (NADH and ferredox) in response to environmental H2 concentrations.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
7. Affordable and Clean Energy
(Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: July 22, 2019
2018 journal article
A synthetic enzymatic pathway for extremely thermophilic acetone production based on the unexpectedly thermostable acetoacetate decarboxylase from Clostridium acetobutylicum
BIOTECHNOLOGY AND BIOENGINEERING, 115(12), 2951–2961.
author keywords: acetone; biotransformations; extreme thermophiles
MeSH headings : Acetone / metabolism; Bacterial Proteins / genetics; Bacterial Proteins / metabolism; Carboxy-Lyases / genetics; Carboxy-Lyases / metabolism; Clostridium acetobutylicum / enzymology; Clostridium acetobutylicum / genetics; Clostridium acetobutylicum / metabolism; Enzyme Stability; Hot Temperature; Metabolic Engineering; Metabolic Networks and Pathways / genetics; Synthetic Biology / methods
TL;DR:
A synthetic enzymatic pathway for acetone production that functions up to at least 70°C in vitro, made possible by the unusual thermostability of Adc from the mesophile C. acetobutylicum, paving the way for bio‐reactive distillation of acetone using a metabolically engineered extreme thermophile as a production host.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
12. Responsible Consumption and Production
(Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: December 3, 2018
2018 review
Biotechnology of extremely thermophilic archaea
[Review of ]. FEMS MICROBIOLOGY REVIEWS, 42(5), 543–578.
author keywords: extremely thermophilic archaea; biotechnology; biocatalysis; biooxidation
MeSH headings : Archaea / genetics; Archaea / physiology; Biotechnology / trends; Hot Temperature; Industrial Microbiology / trends; Metabolic Engineering / trends
TL;DR:
There are barriers and challenges to these organisms reaching their full potential as industrial microorganisms but, if these can be overcome, a new dimension for biotechnology will be forthcoming that strategically exploits biology at high temperatures.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
15. Life on Land
(Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: November 19, 2018
2018 journal article
Complete Genome Sequences of Extremely Thermoacidophilic Metal-Mobilizing Type Strain Members of the Archaeal Family Sulfolobaceae, Acidianus brierleyi DSM-1651, Acidianus sulfidivorans DSM-18786, and Metallosphaera hakonensis DSM-7519
MICROBIOLOGY RESOURCE ANNOUNCEMENTS, 7(2).
TL;DR:
Three closed genomes from two currently defined genera within the family Sulfolobaceae are reported, namely, Acidianus brierleyi DSM-1651T,Acidianus sulfidivorans DSM-18786T, and Metallosphaera hakonensis DSM-7519T.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
15. Life on Land
(Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: December 31, 2018
2018 journal article
Engineering redox-balanced ethanol production in the cellulolytic and extremely thermophilic bacterium, Caldicellulosiruptor bescii
Metabolic Engineering Communications, 7, e00073.
TL;DR:
A strain of C. bescii was engineered using a more stable genetic background than previously reported and heterologously-expressed AdhE resulted in increased ethanol production compared to previous reports, which underlines the importance of redox balancing and paves the way for achieving even higher ethanol titers in C. Bescii.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
7. Affordable and Clean Energy
(OpenAlex)
Sources: Crossref, NC State University Libraries
Added: February 24, 2020
2018 journal article
Genus-Wide Assessment of Lignocellulose Utilization in the Extremely Thermophilic Genus Caldicellulosiruptor by Genomic, Pangenomic, and Metagenomic Analyses
Applied and Environmental Microbiology, 84(9), e02694–17.
Ed(s): I. Cann
author keywords: Caldicellulosiruptor; extreme thermophile; pangenome
MeSH headings : Cellulose / metabolism; Firmicutes / classification; Firmicutes / genetics; Firmicutes / metabolism; Genome, Bacterial; Genomics; Lignin / metabolism; Metagenome; Metagenomics
TL;DR:
It is indicated that microcrystalline cellulose degradation at temperatures above 70°C, based on current information, is limited to certain members of this genus that produce GH48 domain-containing enzymes.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
7. Affordable and Clean Energy
(Web of Science)
12. Responsible Consumption and Production
(Web of Science)
15. Life on Land
(OpenAlex)
Sources: Crossref, NC State University Libraries
Added: July 27, 2021
2018 journal article
Native xylose-inducible promoter expands the genetic tools for the biomass-degrading, extremely thermophilic bacterium Caldicellulosiruptor bescii
EXTREMOPHILES, 22(4), 629–638.
author keywords: Anaerobes; Biodegradation of cellulosic; Biotechnology of thermophiles; Genetics; Molecular biology; Genetics of extremophiles
MeSH headings : Biotransformation; Cellulose / metabolism; Firmicutes / drug effects; Firmicutes / genetics; Firmicutes / growth & development; Firmicutes / metabolism; Industrial Microbiology / methods; L-Lactate Dehydrogenase / genetics; L-Lactate Dehydrogenase / metabolism; Promoter Regions, Genetic; Thermotolerance; Xylose / metabolism; Xylose / pharmacology
TL;DR:
This present study expands the tools available for genetically engineering Caldicellulosiruptor bescii, the most thermophilic cellulose-degrader known growing up to 90 °C on unpretreated plant biomass, by utilizing a native xylose-inducible promoter to control the expression of the reporter gene encoding lactate dehydrogenase.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
7. Affordable and Clean Energy
(Web of Science)
12. Responsible Consumption and Production
(Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018
2018 journal article
Novel multidomain, multifunctional glycoside hydrolases from highly lignocellulolytic Caldicellulosiruptor species
AICHE JOURNAL, 64(12), 4218–4228.
author keywords: Caldicellulosiruptor; lignocellulose; glycoside hydrolase; cellulase
UN Sustainable Development Goal Categories
7. Affordable and Clean Energy
(Web of Science)
12. Responsible Consumption and Production
(Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: November 26, 2018
2018 journal article
Parsing in vivo and in vitro contributions to microcrystalline cellulose hydrolysis by multidomain glycoside hydrolases in the Caldicellulosiruptor bescii secretome
BIOTECHNOLOGY AND BIOENGINEERING, 115(10), 2426–2440.
author keywords: Caldicellulosiruptor berci; cellulase; extreme thermophile; glycoside hydrolase; lignocellulose; secretome
MeSH headings : Cellulose / chemistry; Cellulose / metabolism; Firmicutes / enzymology; Firmicutes / genetics; Glycoside Hydrolases / chemistry; Glycoside Hydrolases / genetics; Glycoside Hydrolases / metabolism; Hydrolysis
TL;DR:
Results suggest that the C. bescii secretome is naturally balanced to achieve optimal synergy for cellulose degradation and reinforce the importance of microbial contributions to microcrystalline cellulose hydrolysis and suggest that mass action effects from glucan fermentation shift equilibria to drive degradation.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
7. Affordable and Clean Energy
(Web of Science)
12. Responsible Consumption and Production
(Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: October 29, 2018
2018 journal article
Secretion and fusion of biogeochemically active archaeal membrane vesicles
GEOBIOLOGY, 16(6), 659–673.
author keywords: archaea; membrane fusion; regulated secretion; thermoacidophiles; vesicles
MeSH headings : Archaea / metabolism; Archaea / physiology; Archaeal Proteins / metabolism
TL;DR:
Membrane vesicles may play a role in modulating energy‐related traits in geochemical environments by fusion‐mediated protein delivery as well as in response to unknown signals and for undefined purposes.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: October 29, 2018
2018 journal article
Sequential processing with fermentative Caldicellulosiruptor kronotskyensis and chemolithoautotrophic Cupriavidus necator for converting rice straw and CO2 to polyhydroxybutyrate
BIOTECHNOLOGY AND BIOENGINEERING, 115(6), 1624–1629.
author keywords: biorefinery; Caldicellulosiruptor; CO2 fixation; Cupriavidus necator; fermentation; lignocellulose
MeSH headings : Animals; Carbon Dioxide / metabolism; Cupriavidus necator / growth & development; Cupriavidus necator / metabolism; Firmicutes / growth & development; Firmicutes / metabolism; Hydroxybutyrates / metabolism; Oryza / metabolism; Plant Stems / metabolism; Polyesters / metabolism
TL;DR:
The sequential coupling of C. kronotskyensis and C. necator provides not only a new biorefinery paradigm characterized by reduced pretreatment and saccharification requirements but also an efficient way for enhancing CO2 fixation.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
7. Affordable and Clean Energy
(Web of Science)
12. Responsible Consumption and Production
(Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018
2018 journal article
Simultaneous biosynthesis of (R)-acetoin and ethylene glycol from D-xylose through in vitro metabolic engineering
Metabolic Engineering Communications, 7, e00074.
TL;DR:
It is shown here that a cell-free bioreaction scheme can generate enantiomerically pure acetoin and EG as co-products from biomass-derived D-xylose, illustrating the potential for production of multiple value-added biomolecules from biomass -based sugars with no ATP requirement.
(via Semantic Scholar)
Sources: Crossref, NC State University Libraries
Added: February 24, 2020
2018 journal article
The diversity and specificity of the extracellular proteome in the cellulolytic bacterium Caldicellulosiruptor bescii is driven by the nature of the cellulosic growth substrate
BIOTECHNOLOGY FOR BIOFUELS, 11.
author keywords: Caldicellulosiruptor bescii; Carbohydrate-active enzymes (CAZymes); Extracellular solute binding proteins (ESBPs); Protein of unknown function (PUF); Glycosyl hydrolases (GH); Mass spectrometry; Switchgrass; Xylan; Avicel; C5 substrates; C6 substrates; Lignocellulosic; Extracellular
TL;DR:
This study reveals the protein membership of the C. bescii secretome and demonstrates its plasticity based on the complexity (mono-/disaccharides vs. polysaccharides) and type of carbon (C5 vs. C6) available to the microorganism.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
7. Affordable and Clean Energy
(Web of Science)
12. Responsible Consumption and Production
(Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018
2018 journal article
Uncoupling Fermentative Synthesis of Molecular Hydrogen from Biomass Formation in Thermotoga maritima
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 84(17).
author keywords: Hyperthermophile; MalK; hydrogen; transient gene inactivation; adaptive laboratory evolution; bacterial genetics; fermentation; hydrogen; Thermotoga maritima
MeSH headings : ATP-Binding Cassette Transporters / genetics; Bacterial Proteins / genetics; Biological Transport / genetics; Biomass; Bioreactors / microbiology; Energy Metabolism / genetics; Energy Metabolism / physiology; Hydrogen / metabolism; L-Lactate Dehydrogenase / genetics; Lactic Acid / biosynthesis; Maltose / metabolism; Thermotoga maritima / genetics; Thermotoga maritima / metabolism
TL;DR:
Cell lines of the extremophile Thermotoga maritima are described that exceed the physiologic limits for H2 formation arising from genetic changes in fermentative metabolism, indicating that a reduced rate of maltose uptake was accompanied by an increase in H2 production, changing fermentation efficiency and shifting energy management.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
12. Responsible Consumption and Production
(Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: October 19, 2018
2017 journal article
Bioavailability of Carbohydrate Content in Natural and Transgenic Switchgrasses for the Extreme Thermophile Caldicellulosiruptor bescii
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 83(17).
author keywords: Caldicellulosiruptor; switchgrass; lignocellulose deconstruction and conversion; extreme thermophiles; lignocellulose
MeSH headings : Biomass; Fermentation; Gram-Positive Bacteria / genetics; Gram-Positive Bacteria / metabolism; Hot Temperature; Lignin / chemistry; Lignin / metabolism; Panicum / chemistry; Panicum / genetics; Panicum / metabolism; Panicum / microbiology; Plants, Genetically Modified / chemistry; Plants, Genetically Modified / genetics; Plants, Genetically Modified / metabolism; Plants, Genetically Modified / microbiology; Polysaccharides / chemistry; Polysaccharides / metabolism
TL;DR:
While C. bescii could significantly solubilize the transgenic switchgrass lines and natural variant tested here, additional or alternative strategies (physical, chemical, enzymatic, and/or genetic) are needed to eliminate recalcitrance.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
7. Affordable and Clean Energy
(Web of Science)
12. Responsible Consumption and Production
(Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018
2017 journal article
C aldicellulosiruptor saccharolyticus transcriptomes reveal consequences of chemical pretreatment and genetic modification of lignocellulose
Microbial Biotechnology, 10(6), 1546–1557.
MeSH headings : Acids / chemistry; Bacterial Proteins / genetics; Bacterial Proteins / metabolism; Biotransformation; Firmicutes / genetics; Firmicutes / metabolism; Lignin / metabolism; Populus / chemistry; Populus / genetics; Populus / metabolism; Populus / microbiology; Transcriptome
TL;DR:
The results here present a new paradigm for assessing plant–microbe interactions that can be deployed as a biological assay to report on the complexity and recalcitrance of plant biomass.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
7. Affordable and Clean Energy
(Web of Science)
12. Responsible Consumption and Production
(Web of Science)
Sources: Crossref, NC State University Libraries
Added: July 21, 2021
2017 journal article
Diversity of bacteria and archaea from two shallow marine hydrothermal vents from Vulcano Island
Extremophiles, 21(4), 733–742.
author keywords: Hyperthermophiles; Archaea; Diversity; Hydrothermal marine shallow vents
MeSH headings : Archaea / classification; Archaea / genetics; Bacteria / classification; Bacteria / genetics; Hydrothermal Vents / microbiology; Italy; Marine Biology; RNA, Ribosomal, 16S / genetics
TL;DR:
Sediment and water samples taken from two shallow marine hydrothermal vents with temperatures of 100 °C at Vulcano Island, Italy showed a high abundance of hyperthermophilic archaea and a comparable diverse archaeal community composition in both samples, and the microbial diversity of the enrichment cultures changed significantly.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
14. Life Below Water
(OpenAlex)
Sources: Crossref, NC State University Libraries
Added: August 3, 2021
2017 journal article
Ethanol production by the hyperthermophilic archaeon Pyrococcus furiosus by expression of bacterial bifunctional alcohol dehydrogenases
Microbial Biotechnology, 10(6), 1535–1545.
TL;DR:
The AOR gene was deleted from P. furiosus to evaluate ethanol production directly from acetyl‐CoA by heterologous expression of the adhE gene from eight thermophilic bacteria, and the highest amount of ethanol was produced whenadhE and adhA from Thermoanaerobacter were co‐expressed.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
7. Affordable and Clean Energy
(Web of Science)
12. Responsible Consumption and Production
(Web of Science)
Sources: Crossref, NC State University Libraries
Added: July 21, 2021
2017 review
Extremely thermophilic energy metabolisms: biotechnological prospects
[Review of ]. CURRENT OPINION IN BIOTECHNOLOGY, 45, 104–112.
MeSH headings : Archaea / classification; Archaea / genetics; Archaea / metabolism; Biotechnology / methods; Carbon Dioxide / metabolism; Chemoautotrophic Growth; Energy Metabolism; Hot Temperature; Lignin / metabolism; Metabolic Engineering
TL;DR:
This review considers recent developments in extreme thermophile biology as they relate to new horizons for energy biotechnology.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
7. Affordable and Clean Energy
(Web of Science)
12. Responsible Consumption and Production
(Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018
2017 journal article
Functional Analysis of the Glucan Degradation Locus in Caldicellulosiruptor bescii Reveals Essential Roles of Component Glycoside Hydrolases in Plant Biomass Deconstruction
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 83(24).
author keywords: Caldicellulosiruptor; extreme thermophile; cellulose degradation; lignocellulose; glycoside hydrolase; cellulase
MeSH headings : Bacterial Proteins / metabolism; Cellulose / chemistry; Firmicutes / genetics; Firmicutes / metabolism; Glucans / metabolism; Glycoside Hydrolases / metabolism; Panicum / chemistry; Populus / chemistry
TL;DR:
It is demonstrated that certain GDL GHs are primarily responsible for the degradation of microcrystalline cellulose-containing substrates by C. bescii and provide new insights into the workings of a novel microbial mechanism for lignocellulose utilization.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
7. Affordable and Clean Energy
(Web of Science)
12. Responsible Consumption and Production
(Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018
2017 journal article
Genome Stability in Engineered Strains of the Extremely Thermophilic Lignocellulose-Degrading Bacterium Caldicellulosiruptor bescii
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 83(14).
author keywords: Southern blotting; genome sequencing; insertion elements; kanamycin resistance
MeSH headings : Bacterial Proteins / genetics; Bacterial Proteins / metabolism; Genetic Engineering; Genome, Bacterial; Genomic Instability; Gram-Positive Bacteria / genetics; Gram-Positive Bacteria / metabolism; Hot Temperature; Lignin / metabolism
TL;DR:
It is shown that strains derived from the genetic background containing a random deletion in uracil biosynthesis genes (pyrFA) have a dramatic increase in the number of single nucleotide polymorphisms, insertions/deletions, and ISCbe4 insertion elements in their genomes compared to the wild type.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
7. Affordable and Clean Energy
(Web of Science)
12. Responsible Consumption and Production
(Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018
2017 journal article
Impact of growth mode, phase, and rate on the metabolic state of the extremely thermophilic archaeon Pyrococcus furiosus
BIOTECHNOLOGY AND BIOENGINEERING, 114(12), 2947–2954.
author keywords: continuous culture; growth phase; growth rate; hyperthermophiles; Pyrococcus furiosus; transcriptome
MeSH headings : Archaeal Proteins / metabolism; Batch Cell Culture Techniques / methods; Cell Proliferation / physiology; Energy Metabolism / physiology; Pyrococcus furiosus / growth & development; Pyrococcus furiosus / metabolism; Transcriptome / physiology
TL;DR:
The results here suggest that cellular processes involved in carbon and electron flux in P. furiosus were significantly impacted by growth mode, phase and rate, factors that need to be taken into account when developing successful metabolic engineering strategies.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
7. Affordable and Clean Energy
(Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018
2017 review
Physiological, metabolic and biotechnological features of extremely thermophilic microorganisms
[Review of ]. WILEY INTERDISCIPLINARY REVIEWS-SYSTEMS BIOLOGY AND MEDICINE, 9(3).
MeSH headings : Biocatalysis; Carbohydrate Metabolism; Carbon Dioxide / metabolism; Glycolysis; Metabolic Engineering; Metals / chemistry; Metals / metabolism; Sulfolobales / metabolism; Sulfur / metabolism; Thermoanaerobacter / metabolism; Thermococcales / metabolism; Thermus / metabolism
TL;DR:
The bacterial genera Caldicellulosiruptor, Thermotoga and Thermus, and the archaea belonging to the orders Thermococcales and Sulfolobales, are among the most studied extreme thermophiles to date.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
14. Life Below Water
(OpenAlex)
15. Life on Land
(Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018
2017 report
Sequestration of CO2 with H2 into useful products
(US Patent No. 9,587,256).
Source: NC State University Libraries
Added: August 27, 2021
2017 article
The renaissance of life near the boiling point - at last, genetics and metabolic engineering
Adams, M. W. W., & Kelly, R. M. (2017, January). MICROBIAL BIOTECHNOLOGY, Vol. 10, pp. 37–39.
MeSH headings : Biotechnology / methods; Hot Temperature; Industrial Microbiology / methods; Metabolic Engineering / methods
TL;DR:
The prospects for biotechnology of extreme thermophilic microorganisms are discussed here and the implications are discussed for science, medicine, and society.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
7. Affordable and Clean Energy
(Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018
2017 journal article
Two Distinct alpha-L-Arabinofuranosidases in Caldicellulosiruptor Species Drive Degradation of Arabinose-Based Polysaccharides
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 83(13).
author keywords: Arabinofuranosidase; bioenergy; glycoside hydrolase; hyperthermophiles; synergism
MeSH headings : Arabinose / metabolism; Bacterial Proteins / chemistry; Bacterial Proteins / genetics; Bacterial Proteins / metabolism; Firmicutes / chemistry; Firmicutes / enzymology; Firmicutes / genetics; Firmicutes / metabolism; Glycoside Hydrolases / chemistry; Glycoside Hydrolases / genetics; Glycoside Hydrolases / metabolism; Hydrogen-Ion Concentration; Molecular Weight; Polysaccharides / metabolism; Substrate Specificity
TL;DR:
The data suggest that plant biomass degradation in Caldicellulosiruptor species involves extracellular XynF that acts synergistically with other hemicellULases to digest arabino-polysaccharides that are subsequently transported and degraded further by intracellular AbF51 to produce short-chain arabinos sugars.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
7. Affordable and Clean Energy
(Web of Science)
12. Responsible Consumption and Production
(Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018
2017 journal article
VapC toxins drive cellular dormancy under uranium stress for the extreme thermoacidophile Metallosphaera prunae
ENVIRONMENTAL MICROBIOLOGY, 19(7), 2831–2842.
MeSH headings : Archaeal Proteins / genetics; Archaeal Proteins / metabolism; Bacterial Toxins / genetics; Bacterial Toxins / metabolism; Gene Expression Regulation, Bacterial / physiology; RNA Stability / physiology; Sulfolobaceae / genetics; Sulfolobaceae / growth & development; Sulfolobaceae / metabolism; Transcriptome; Uranium / metabolism
TL;DR:
M. prunae utilizes VapC toxins for post-transcriptional regulation under uranium stress to enter a cellular dormant state, thereby providing an adaptive response to what would otherwise be a deleterious environmental perturbation.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018
2016 journal article
A Highly Thermostable Kanamycin Resistance Marker Expands the Tool Kit for Genetic Manipulation of Caldicellulosiruptor bescii
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 82(14), 4421–4428.
MeSH headings : Firmicutes / genetics; Firmicutes / radiation effects; Genetics, Microbial / methods; Genomic Instability / radiation effects; Hot Temperature; Kanamycin Resistance; Molecular Biology / methods; Selection, Genetic
TL;DR:
A selection system for C. bescii using the antibiotic kanamycin is developed and it is shown that, in combination with the biosynthetic gene marker, it can be used to efficiently delete genes in this organism.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
7. Affordable and Clean Energy
(Web of Science)
12. Responsible Consumption and Production
(Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018
2016 journal article
Ancillary contributions of heterologous biotin protein ligase and carbonic anhydrase for CO2 incorporation into 3-hydroxypropionate by metabolically engineered Pyrococcus furiosus
Biotechnology and Bioengineering, 113(12), 2652–2660.
author keywords: biotin protein ligase; carboxylase; carbon dioxide; Pyrococcus furiosus; 3-hydroxypropionate; Metallosphaera sedula
MeSH headings : Carbon Dioxide / chemistry; Carbon Dioxide / metabolism; Carbon-Nitrogen Ligases / metabolism; Carbonic Anhydrases / genetics; Escherichia coli Proteins / metabolism; Lactic Acid / analogs & derivatives; Lactic Acid / biosynthesis; Lactic Acid / chemistry; Metabolic Engineering / methods; Protein Engineering / methods; Pyrococcus furiosus / physiology; Recombinant Proteins / genetics; Recombinant Proteins / metabolism; Repressor Proteins / metabolism; Sulfolobaceae / genetics; Sulfolobaceae / metabolism
TL;DR:
The results illustrate the ancillary benefits of accessory enzymes for incorporating CO2 into 3HP production in metabolically engineered P. furiosus, and hint at the important role that CA and BPL likely play in the native 3HP/4HB pathway in M. sedula.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
6. Clean Water and Sanitation
(OpenAlex)
Sources: Crossref, NC State University Libraries
Added: December 31, 2020
2016 chapter
Extreme Thermophiles as Metabolic Engineering Platforms: Strategies and Current Perspective
In C. Wittmann & J. C. Liao (Eds.), Industrial Biotechnology: Microorganisma (Vol. 1, pp. 505–580).
Ed(s): C. Wittmann & J. Liao
Sources: Crossref, NC State University Libraries
Added: July 30, 2021
2016 journal article
Heterologous Production of an Energy-Conserving Carbon Monoxide Dehydrogenase Complex in the Hyperthermophile Pyrococcus furiosus
FRONTIERS IN MICROBIOLOGY, 7.
author keywords: hyperthermophile; archaea; hydrogen; carbon monoxide; energy; anaerobic respiration; Thermococcales
TL;DR:
The functional transfer of CO utilization between Thermococcus and Pyrococcus species demonstrated herein is representative of the horizontal gene transfer of an environmentally relevant metabolic capability.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
7. Affordable and Clean Energy
(Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018
2016 journal article
Multidomain, Surface Layer-associated Glycoside Hydrolases Contribute to Plant Polysaccharide Degradation by Caldicellulosiruptor Species
JOURNAL OF BIOLOGICAL CHEMISTRY, 291(13), 6732–6747.
author keywords: biofuel; cell surface; cell surface enzyme; enzyme; glycoside hydrolase; plant cell wall; Caldicellulosiruptor; S-layer; lignocellulose
MeSH headings : Bacterial Proteins / chemistry; Bacterial Proteins / genetics; Bacterial Proteins / metabolism; Cloning, Molecular; Clostridiales / chemistry; Clostridiales / classification; Clostridiales / enzymology; Escherichia coli / genetics; Escherichia coli / metabolism; Gene Expression; Genome, Bacterial; Glucans / metabolism; Glycoside Hydrolases / chemistry; Glycoside Hydrolases / genetics; Glycoside Hydrolases / metabolism; Kinetics; Mutation; Phylogeny; Polysaccharides / metabolism; Protein Binding; Protein Engineering; Protein Structure, Tertiary; Recombinant Proteins / chemistry; Recombinant Proteins / genetics; Recombinant Proteins / metabolism; Substrate Specificity; Wood / metabolism; Xylans / metabolism
TL;DR:
The results here provide new insights into the architecture and role of SLHdomain GHs and demonstrate that hemicellulose degradation can be enhanced through non-native SLH domain GHs engineered into the genomes of Caldicelluosiruptor species.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
7. Affordable and Clean Energy
(Web of Science)
12. Responsible Consumption and Production
(Web of Science)
15. Life on Land
(OpenAlex)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018
2016 journal article
Reaction kinetic analysis of the 3-hydroxypropionate/4-hydroxybutyrate CO2 fixation cycle in extremely thermoacidophilic archaea
METABOLIC ENGINEERING, 38, 446–463.
author keywords: CO2 fixation; 3-hydroxypropionate; 4-hydroxybutyrate; Metallosphaera sedula
MeSH headings : Archaea / metabolism; Carbon Dioxide / metabolism; Extremophiles / metabolism; Hydroxybutyrates / metabolism; Kinetics; Lactic Acid / analogs & derivatives; Lactic Acid / metabolism; Metabolic Clearance Rate; Metabolic Flux Analysis / methods; Metabolic Networks and Pathways / physiology; Models, Biological; Signal Transduction / physiology; Sulfolobaceae / metabolism
TL;DR:
A reaction kinetics model was developed to examine the biological and biotechnological attributes of the 3HP/4HB cycle as it operates in Metallosphaera sedula and assess metabolic engineering strategies for incorporating CO2 into chemical intermediates and products of bioteschnological importance: acetyl-CoA, succinate, and 3-hydroxypropionate.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
15. Life on Land
(Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018
2016 journal article
Temperature-dependent acetoin production by Pyrococcus furiosus is catalyzed by a biosynthetic acetolactate synthase and its deletion improves ethanol production
METABOLIC ENGINEERING, 34, 71–79.
author keywords: Archaea; Hyperthermophile; Genetic engineering; Acetolactate synthase; Acetoin; Ethanol
MeSH headings : Acetoin / metabolism; Acetolactate Synthase / biosynthesis; Acetolactate Synthase / genetics; Catalysis; Ethanol / isolation & purification; Ethanol / metabolism; Gene Deletion; Genetic Enhancement / methods; Metabolic Engineering / methods; Metabolic Networks and Pathways / physiology; Pyrococcus furiosus / genetics; Pyrococcus furiosus / metabolism; Temperature
TL;DR:
It is shown that P. furiosus produces acetoin (3-hydroxybutanone) as a major product at temperatures below 80°C and deletion of the als gene abolishes acetoin production, and that a strain of P.furiosus containing a novel pathway for ethanol production significantly improved the yield of ethanol.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
7. Affordable and Clean Energy
(Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018
2016 journal article
Transcriptomes of the Extremely Thermoacidophilic Archaeon Metallosphaera sedula Exposed to Metal "Shock" Reveal Generic and Specific Metal Responses
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 82(15), 4613–4627.
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018
2015 journal article
A New Class of Tungsten-Containing Oxidoreductase in Caldicellulosiruptor, a Genus of Plant Biomass-Degrading Thermophilic Bacteria
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 81(20), 7339–7347.
MeSH headings : Aldehydes / metabolism; Bacterial Proteins / metabolism; Gram-Positive Bacteria / enzymology; Gram-Positive Bacteria / metabolism; Oxidation-Reduction; Oxidoreductases / chemistry; Oxidoreductases / metabolism; Pyrococcus furiosus / enzymology; Pyrococcus furiosus / metabolism; Tungsten / metabolism
TL;DR:
Evidence was uncovered to suggest that the primary redox metabolism of C. bescii has a completely uncharacterized aspect involving tungsten, a rarely used element in biology.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
7. Affordable and Clean Energy
(Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018
2015 journal article
A hybrid synthetic pathway for butanol production by a hyperthermophilic microbe
METABOLIC ENGINEERING, 27, 101–106.
author keywords: Butanol; Biofuels; Hyperthermophile; Module; Hybrid; Pathway
MeSH headings : 1-Butanol / metabolism; Acetyl Coenzyme A / genetics; Acetyl Coenzyme A / metabolism; Metabolic Engineering / methods; Thermoanaerobacterium / genetics; Thermoanaerobacterium / metabolism
TL;DR:
A synthetic metabolic pathway assembled from genes obtained from three different sources for conversion of acetyl-CoA to 1-butanol was demonstrated near 70°C in a microorganism that grows optimally near 100°C, and the module could also be used in thermophiles capable of degrading plant biomass.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
12. Responsible Consumption and Production
(Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018
2015 journal article
A mutant ('lab strain') of the hyperthermophilic archaeon Pyrococcus furiosus, lacking flagella, has unusual growth physiology
EXTREMOPHILES, 19(2), 269–281.
author keywords: Pyrococcus furiosus; Hyperthermophile; Archaeon; Growth physiology; Flagella
TL;DR:
It is indicated that flagella play a key role, beyond motility, in the growth physiology of P. furiosus and, unlike the WT, showed minimal evidence of exopolysaccharide-based cell aggregation in post-exponential phase.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018
2015 journal article
Alcohol Selectivity in a Synthetic Thermophilic n-Butanol Pathway Is Driven by Biocatalytic and Thermostability Characteristics of Constituent Enzymes
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 81(20), 7187–7200.
MeSH headings : 1-Butanol / metabolism; Acetyl-CoA C-Acyltransferase / metabolism; Acyl Coenzyme A / metabolism; Alcohol Dehydrogenase / metabolism; Alcohol Oxidoreductases / metabolism; Aldehyde Oxidoreductases / metabolism; Biocatalysis; Chromatography, Gas; Clostridium acetobutylicum / metabolism; Clostridium thermocellum / metabolism; Thermoanaerobacter / metabolism
TL;DR:
More-selective thermophilic versions of Bad, Bdh, and AdhE are needed to fully exploit biocatalytic n-butanol production at elevated temperatures.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
12. Responsible Consumption and Production
(Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018
2015 journal article
Bioprocessing analysis of Pyrococcus furiosus strains engineered for CO2-based 3-hydroxypropionate production
BIOTECHNOLOGY AND BIOENGINEERING, 112(8), 1533–1543.
author keywords: 3-hydroxypropionate; CO2 fixation; Metallosphaera sedula; Pyrococcus furiosus
MeSH headings : Bioreactors / microbiology; Carbon Dioxide / metabolism; Gene Expression Profiling; Hot Temperature; Lactic Acid / analogs & derivatives; Lactic Acid / metabolism; Maltose / metabolism; Metabolic Engineering / methods; Metabolic Networks and Pathways / genetics; Pyrococcus furiosus / genetics; Pyrococcus furiosus / metabolism; Pyrococcus furiosus / radiation effects; Sulfolobaceae / genetics
TL;DR:
The results here support the prospects of using thermally diverse sources of pathways and enzymes in metabolically engineered strains designed for product formation at sub‐optimal growth temperatures.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
7. Affordable and Clean Energy
(Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018
2015 journal article
Comparative Analysis of Extremely Thermophilic Caldicellulosiruptor Species Reveals Common and Unique Cellular Strategies for Plant Biomass Utilization
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 81(20), 7159–7170.
MeSH headings : Bacteria / metabolism; Biomass; Cellulase / metabolism; Panicum / enzymology; Panicum / metabolism; Plants / enzymology
TL;DR:
Results here show that capacity for plant biomass deconstruction varies across Caldicellulosiruptor species and depends in a complex way on GH genome inventory, substrate composition, and gene regulation.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
7. Affordable and Clean Energy
(Web of Science)
12. Responsible Consumption and Production
(Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018
2015 journal article
Complete Genome Sequences of Caldicellulosiruptor sp. Strain Rt8.B8, Caldicellulosiruptor sp. Strain Wai35.B1, and “ Thermoanaerobacter cellulolyticus ”
Complete Genome Sequences of Caldicellulosiruptor sp. Strain Rt8.B8, Caldicellulosiruptor sp. Strain Wai35.B1, and “ Thermoanaerobacter cellulolyticus .” Genome Announcements, 3(3).
TL;DR:
Gains in genome sequences for three additional Caldicellulosiruptor species are reported, including Rt8.B8 DSM 8990 (New Zealand), Wai35.B1 DSM 8977 ( new Zealand), and “Thermoanaerobacter cellulolyticus” strain NA10 DSM 8991 (Japan).
(via Semantic Scholar)
UN Sustainable Development Goal Categories
7. Affordable and Clean Energy
(Web of Science)
12. Responsible Consumption and Production
(Web of Science)
Sources: Crossref, NC State University Libraries
Added: July 21, 2021
2015 journal article
Discrete and Structurally Unique Proteins (Tapirins) Mediate Attachment of Extremely Thermophilic Caldicellulosiruptor Species to Cellulose
JOURNAL OF BIOLOGICAL CHEMISTRY, 290(17), 10645–10656.
MeSH headings : Adsorption; Bacteria / genetics; Bacteria / metabolism; Bacteria / ultrastructure; Bacterial Adhesion / physiology; Bacterial Proteins / chemistry; Bacterial Proteins / genetics; Bacterial Proteins / metabolism; Binding Sites; Cellulose / metabolism; Fimbriae, Bacterial / metabolism; Genes, Bacterial; Models, Molecular; Phylogeny; Plants / microbiology; Protein Structure, Tertiary; Recombinant Proteins / chemistry; Recombinant Proteins / genetics; Recombinant Proteins / metabolism
TL;DR:
Structurally unique, discrete proteins (tāpirins), not directly associated with cellulases, that mediate attachment to cellulose by species in the noncellulosomal, extremely thermophilic bacterial genus Caldicellulosiruptor are identified and define a new class of polysaccharide adhesins.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
7. Affordable and Clean Energy
(Web of Science)
12. Responsible Consumption and Production
(Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018
2015 review
Extremely thermophilic microorganisms as metabolic engineering platforms for production of fuels and industrial chemicals
[Review of ]. FRONTIERS IN MICROBIOLOGY, 6.
author keywords: extreme thermophiles; metabolic engineering; bio-based chemicals; genetics; biotechnology
TL;DR:
The issues and opportunities for extremely thermophilic metabolic engineering platforms are considered with an eye toward potential technological advantages for high temperature industrial biotechnology.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
7. Affordable and Clean Energy
(Web of Science)
12. Responsible Consumption and Production
(Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018
2015 report
Genetically engineered microbes and methods for converting organic acid to alcohols
(US Patent Application No. 14/670,601).
Source: NC State University Libraries
Added: August 27, 2021
2015 journal article
Machine learning reveals sex-specific 17β-estradiol-responsive expression patterns in white perch (Morone americana) plasma proteins
PROTEOMICS, 15(15), 2678–2690.
Contributors: J. Schilling n, A. Nepomuceno n, A. Planchart n , J. Yoder n , n , D. Muddiman n , H. Daniels n, N. Hiramatsu*, B. Reading n
author keywords: Animal proteomics; Endocrine disrupting compounds; Machine learning; Plasma; Semiquantitative proteomics; Support vector machines
MeSH headings : Animals; Bass / metabolism; Blood Proteins / metabolism; Blotting, Western; Chromatography, Liquid; Estradiol / pharmacology; Estrogens / pharmacology; Female; Fish Proteins / metabolism; Kininogens / metabolism; Male; Nanotechnology / methods; Proteome / drug effects; Proteome / metabolism; Proteomics / methods; Sex Factors; Support Vector Machine; Tandem Mass Spectrometry; Vitellogenins / metabolism
TL;DR:
This is the first report that Eltd1 and Kng1 may be E2‐responsive proteins in fishes and therefore may be useful indicators of estrogen induction in fishes.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
14. Life Below Water
(Web of Science)
Sources: Web Of Science, NC State University Libraries, ORCID, Crossref
Added: August 6, 2018
2015 review
The Confluence of Heavy Metal Biooxidation and Heavy Metal Resistance: Implications for Bioleaching by Extreme Thermoacidophiles
[Review of ]. MINERALS, 5(3), 397–451.
author keywords: extreme thermoacidophiles; bioleaching; heavy metal resistance; heavy metal biooxidation; archaea
TL;DR:
Here, extreme thermoacidophiles are examined from the perspectives of biodiversity, heavy metal biooxidation, metal resistance mechanisms, microbe-solid interactions, and application of these archaea in biomining operations.
(via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018
2014 journal article
Conversion of 4-Hydroxybutyrate to Acetyl Coenzyme A and Its Anapleurosis in the Metallosphaera sedula 3-Hydroxypropionate/4-Hydroxybutyrate Carbon Fixation Pathway
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 80(8), 2536–2545.
MeSH headings : Acetyl Coenzyme A / metabolism; Carbon Cycle; Escherichia coli / genetics; Gene Expression Profiling; Metabolic Networks and Pathways / genetics; Recombinant Proteins / genetics; Recombinant Proteins / isolation & purification; Recombinant Proteins / metabolism; Sodium Oxybate / metabolism; Sulfolobaceae / metabolism; Transcriptome
TL;DR:
Transcriptomic analysis revealed the importance of the 3HP/4HB pathway in supplying acetyl-CoA to anabolic pathways generating intermediates in M. sedula metabolism, and provided the framework for examining connections between CO2 fixation and central metabolism in the sedula.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
6. Clean Water and Sanitation
(OpenAlex)
15. Life on Land
(Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018
2014 journal article
Cross-linked Polymer Nanofibers for Hyperthermophilic Enzyme Immobilization: Approaches to Improve Enzyme Performance
ACS APPLIED MATERIALS & INTERFACES, 6(15), 11899–11906.
author keywords: enzyme immobilization; nanofibers; electrospinning; biocatalysts; mass transfer
MeSH headings : Catalysis; Cross-Linking Reagents / chemistry; Electrochemistry; Enzymes, Immobilized / chemistry; Escherichia coli / metabolism; Hydrogen-Ion Concentration; Kinetics; Microscopy, Electron, Scanning; Nanofibers / chemistry; Nanotechnology / methods; Polymers / chemistry; Polyvinyl Alcohol / chemistry; Solvents / chemistry; Temperature; Thermotoga maritima / metabolism
TL;DR:
An enzyme immobilization method effective at elevated temperatures (up to 105 °C) and sufficiently robust for hyperthermophilic enzymes is reported and Improvements in the apparent activity can be achieved by incorporating a cryoprotectant during immobilization to prevent enzyme deactivation.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
6. Clean Water and Sanitation
(OpenAlex)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018
2014 journal article
Deletion of acetyl-CoA synthetases I and II increases production of 3-hydroxypropionate by the metabolically-engineered hyperthermophile Pyrococcus furiosus
METABOLIC ENGINEERING, 22, 83–88.
author keywords: Electrofuels; Metallosphaera sedula; Acetyl-CoA synthetase; 3-Hydroxypropionate
MeSH headings : Archaeal Proteins; Coenzyme A Ligases; Gene Deletion; Lactic Acid / analogs & derivatives; Lactic Acid / metabolism; Metabolic Engineering / methods; Pyrococcus furiosus / genetics; Pyrococcus furiosus / metabolism
TL;DR:
It is shown that strains of MW56 lacking the α-subunit of either of the two ACSs previously characterized from P. furiosus exhibit a three-fold increase in specific 3-HP production.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
7. Affordable and Clean Energy
(Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018
2014 journal article
Engineering Hydrogen Gas Production from Formate in a Hyperthermophile by Heterologous Production of an 18-Subunit Membrane-bound Complex
JOURNAL OF BIOLOGICAL CHEMISTRY, 289(5), 2873–2879.
author keywords: Archaea; Enzymes; Genetics; Metabolic Engineering; Microbiology; Hydrogen; Hyperthermophiles
MeSH headings : Biofuels / microbiology; Formates / metabolism; Gases / metabolism; Genes, Bacterial / genetics; Hydrogen / metabolism; Membrane Proteins / genetics; Membrane Proteins / metabolism; Metabolic Engineering / methods; Multienzyme Complexes / genetics; Multienzyme Complexes / metabolism; Operon / genetics; Pyrococcus furiosus / enzymology; Pyrococcus furiosus / genetics; Thermococcus / enzymology; Thermococcus / genetics
TL;DR:
The model hyperthermophile Pyrococcus furiosus, which grows optimally near 100 °C by fermenting sugars to produce H2, has been engineered to also efficiently convert formate to H1, demonstrating the versatility of this model organism for metabolic engineering purposes.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
7. Affordable and Clean Energy
(Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018
2014 journal article
Nanofibrous membranes for single-step immobilization of hyperthermophilic enzymes
JOURNAL OF MEMBRANE SCIENCE, 472, 251–260.
author keywords: Enzyme immobilization; Nanofibers; Electrospinning; Chemical crosslinking; Hyperthermophilic enzymes
TL;DR:
Analysis of this immobilization method indicates that the apparent decrease in specific activity could be attributed to enzyme deactivation arising from the crosslinking reaction, whereas mass transfer limits the apparent activity using alternative two-step immobilization methods.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018
2014 journal article
Production of lignofuels and electrofuels by extremely thermophilic microbes
Biofuels, 5(5), 499–515.
author keywords: extreme thermophiles; Pyrococcus furiosus; Metallosphaera sedula; Caldicellulosiruptor; biofuels; electrofuels; CO2 fixation; lignocellulose
TL;DR:
Recent progress towards the synthesis of lignofuels and electrofuels by extremely thermophilic microorganisms is reviewed, including temperature-regulated biosynthesis of industrial organic chemicals and liquid fuel molecules.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
7. Affordable and Clean Energy
(Web of Science)
12. Responsible Consumption and Production
(Web of Science)
Sources: Crossref, NC State University Libraries
Added: February 24, 2020
2014 journal article
Role of an Archaeal PitA Transporter in the Copper and Arsenic Resistance of Metallosphaera sedula, an Extreme Thermoacidophile
JOURNAL OF BACTERIOLOGY, 196(20), 3562–3570.
MeSH headings : Archaeal Proteins / genetics; Archaeal Proteins / metabolism; Arsenic / metabolism; Arsenic / toxicity; Biological Transport; Copper / metabolism; Copper / toxicity; Gene Expression Regulation, Archaeal / physiology; Genome, Archaeal; Molecular Sequence Data; Sulfolobaceae / drug effects; Sulfolobaceae / metabolism
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018
2014 journal article
Single gene insertion drives bioalcohol production by a thermophilic archaeon
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 111(49), 17618–17623.
author keywords: Archaea; metabolic engineering; hyperthermophile; carbon monoxide; aldehydes
MeSH headings : Acetates / chemistry; Aldehyde Oxidoreductases / genetics; Aldehyde Oxidoreductases / metabolism; Aldehydes / chemistry; Biofuels; Carbon Monoxide / chemistry; Escherichia coli / metabolism; Ethanol / chemistry; Fermentation; Maltose / chemistry; Mutagenesis, Insertional; Protein Engineering / methods; Pyrococcus furiosus / genetics; Temperature
TL;DR:
This study engineered the model hyperthermophile Pyrococcus furiosus to produce various alcohols from their corresponding organic acids by constructing a synthetic route termed the AOR/AdhA pathway, a potentially game-changing strategy for syngas fermentation, especially in combination with carbon chain elongation pathways.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
7. Affordable and Clean Energy
(Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018
2014 chapter
The Extremely Thermophilic Genus Caldicellulosiruptor: Physiological and Genomic Characteristics for Complex Carbohydrate Conversion to Molecular Hydrogen
In Microbial BioEnergy: Hydrogen Production (pp. 177–195).
TL;DR:
Extremely thermophilic, carbohydrate-utilizing bacteria from the genus Caldicellulosiruptor should be considered for biohydrogen production to take advantage of their broad growth substrate range and high substrate conversion efficiency.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
7. Affordable and Clean Energy
(Web of Science)
12. Responsible Consumption and Production
(Web of Science)
Sources: Crossref, NC State University Libraries
Added: July 21, 2021
2014 chapter
The Order Thermococcales and the Family Thermococcaceae
In The Prokaryotes (pp. 363–383).
Sources: Crossref, NC State University Libraries
Added: July 21, 2021
2014 review
Thermophilic lignocellulose deconstruction
[Review of ]. FEMS MICROBIOLOGY REVIEWS, 38(3), 393–448.
author keywords: thermophilic microorganisms; cellulosome; lignocellulose deconstruction; carbohydrate-active enzymes; systems biology; bioenergy
MeSH headings : Biofuels; Cellulosomes / genetics; Cellulosomes / metabolism; Gram-Positive Endospore-Forming Bacteria / classification; Gram-Positive Endospore-Forming Bacteria / enzymology; Gram-Positive Endospore-Forming Bacteria / genetics; Hot Temperature; Lignin / metabolism; Molecular Sequence Data; Plants / metabolism; Soil Microbiology
TL;DR:
Plant-biomass-deconstructing thermophilic bacteria from the genera Clostridium and Caldicellulosiruptor, which have potential as metabolic engineering platforms for producing biofuels, are compared and contrasted from a systems biology perspective.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
7. Affordable and Clean Energy
(Web of Science)
12. Responsible Consumption and Production
(Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018
2013 journal article
A thermophile under pressure: Transcriptional analysis of the response of Caldicellulosiruptor saccharolyticus to different H-2 partial pressures
INTERNATIONAL JOURNAL OF HYDROGEN ENERGY, 38(4), 1837–1849.
author keywords: Caldicellulosiruptor saccharolyticus; Hydrogen partial pressure; Chemostat; Metabolic shift; Gene expression; Rex transcriptional regulator
TL;DR:
Transmission data provide evidence for the involvement of the redox sensing Rex protein in gene regulation under high P H 2 cultivation conditions and differential transcription was observed for genes involved in carbon metabolism, fatty acid biosynthesis and several transport systems.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
12. Responsible Consumption and Production
(Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018
2013 review
Biological conversion of carbon dioxide and hydrogen into liquid fuels and industrial chemicals
[Review of ]. CURRENT OPINION IN BIOTECHNOLOGY, 24(3), 376–384.
MeSH headings : Bioelectric Energy Sources; Biotechnology / methods; Carbon Cycle / genetics; Carbon Dioxide / metabolism; Chemical Industry; Hydrogen / metabolism; Metabolic Engineering / methods
TL;DR:
This review examines the current landscape of electrofuel projects with a focus on hydrogen-utilizing organisms covering the biochemistry of hydrogenases and carbonic anhydrases, kinetic and energetic analyses of the known carbon fixation pathways, and the state of genetic systems for current and prospective electrofuel-producing microorganisms.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
7. Affordable and Clean Energy
(Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018
2013 journal article
Carbohydrate and lignin are simultaneously solubilized from unpretreated switchgrass by microbial action at high temperature
Energy & Environmental Science, 6(7), 2186.
TL;DR:
This unexpected finding of simultaneous lignin and carbohydrate solubilization bodes well for industrial conversion by extremely thermophilic microbes of biomass that requires limited or, more importantly, no chemical pretreatment.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
7. Affordable and Clean Energy
(Web of Science)
12. Responsible Consumption and Production
(Web of Science)
Source: Crossref
Added: February 24, 2020
2013 journal article
Exploiting microbial hyperthermophilicity to produce an industrial chemical, using hydrogen and carbon dioxide
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 110(15), 5840–5845.
author keywords: anaerobe; archaea; biotechnology; metabolic engineering; thermophile
MeSH headings : Carbohydrates / chemistry; Carbon Dioxide / chemistry; Gases; Genetic Engineering; Hydrogen / chemistry; Industrial Microbiology / methods; Lactic Acid / analogs & derivatives; Lactic Acid / biosynthesis; Lactic Acid / chemistry; Operon; Polymerase Chain Reaction; Pyrococcus furiosus / genetics; Pyrococcus furiosus / growth & development; Pyrococcus furiosus / metabolism; Temperature
TL;DR:
A unique temperature-dependent approach is described that confers on a microorganism (the archaeon Pyrococcus furiosus, which grows optimally on carbohydrates at 100°C) the capacity to use carbon dioxide, a reaction that it does not accomplish naturally.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
7. Affordable and Clean Energy
(Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018
2013 journal article
Role of 4-Hydroxybutyrate-CoA Synthetase in the CO2 Fixation Cycle in Thermoacidophilic Archaea
JOURNAL OF BIOLOGICAL CHEMISTRY, 288(6), 4012–4022.
MeSH headings : Archaeal Proteins / chemistry; Archaeal Proteins / genetics; Archaeal Proteins / metabolism; Carbon Dioxide / metabolism; Catalysis; Catalytic Domain; Coenzyme A-Transferases / chemistry; Coenzyme A-Transferases / genetics; Coenzyme A-Transferases / metabolism; Genome, Archaeal / physiology; Hydroxybutyrates / chemistry; Hydroxybutyrates / metabolism; Sulfolobaceae / enzymology; Sulfolobaceae / genetics
TL;DR:
Transcriptomic analysis of cells grown under strict H2-CO2 autotrophy was consistent with the involvement of Msed_0406 and Msed-0394, and the identification of the 4-HB CoA synthetase now completes the set of enzymes comprising the 3HP/4HB cycle.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
15. Life on Land
(Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018
2013 journal article
Stationary Phase and Nutrient Levels Trigger Transcription of a Genomic Locus Containing a Novel Peptide (TM1316) in the Hyperthermophilic Bacterium Thermotoga maritima
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 79(21), 6637–6646.
MeSH headings : Carbon / metabolism; Gene Expression Regulation, Bacterial / genetics; Gene Expression Regulation, Bacterial / physiology; Genetic Loci / genetics; Microscopy, Fluorescence; Molecular Sequence Annotation / methods; Nitrogen / metabolism; Operon / genetics; Peptides / genetics; Thermotoga maritima / genetics; Thermotoga maritima / growth & development; Thermotoga maritima / metabolism
TL;DR:
The onset of stationary phase in T. maritima was accompanied by significant morphological changes and upregulation of several ORFs located in the TM1298-TM1336 genome locus, which includes a putative 31-amino-acid peptide (TM1316) that was the most highly transcribed gene in the transcriptome during stationary phase.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
14. Life Below Water
(OpenAlex)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018
2012 journal article
Caldicellulosiruptor Core and Pangenomes Reveal Determinants for Noncellulosomal Thermophilic Deconstruction of Plant Biomass
JOURNAL OF BACTERIOLOGY, 194(15), 4015–4028.
MeSH headings : Adhesins, Bacterial / analysis; Adhesins, Bacterial / genetics; Biomass; Carbohydrate Metabolism; Cellulases / analysis; Cellulases / genetics; Cellulose / metabolism; Genetic Variation; Genome, Bacterial; Gram-Positive Bacteria / enzymology; Gram-Positive Bacteria / genetics; Metabolic Networks and Pathways / genetics; Plants / chemistry; Proteome / analysis
TL;DR:
Taking into account the core genomes, pangenomes, and individual genomes, the ancestral Caldicellulosiruptor was likely cellulolytic and evolved, in some cases, into species that lost the ability to degrade crystalline cellulose while maintaining the capacity to hydrolyze amorphous cellulose and hemICEllulose.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
7. Affordable and Clean Energy
(Web of Science)
12. Responsible Consumption and Production
(Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018
2012 journal article
Epimerase (Msed_0639) and Mutase (Msed_0638 and Msed_2055) Convert (S)-Methylmalonyl-Coenzyme A (CoA) to Succinyl-CoA in the Metallosphaera sedula 3-Hydroxypropionate/4-Hydroxybutyrate Cycle
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 78(17), 6194–6202.
MeSH headings : Acyl Coenzyme A / metabolism; Autotrophic Processes; Biotransformation; Carbon Dioxide / metabolism; Cobamides / metabolism; Escherichia coli / genetics; Gene Expression; Gene Expression Profiling; Heterotrophic Processes; Hydroxybutyrates / metabolism; Methylmalonyl-CoA Mutase / chemistry; Methylmalonyl-CoA Mutase / genetics; Methylmalonyl-CoA Mutase / metabolism; Protein Multimerization; Racemases and Epimerases / chemistry; Racemases and Epimerases / genetics; Racemases and Epimerases / metabolism; Recombinant Proteins / genetics; Recombinant Proteins / metabolism; Sulfolobaceae / enzymology; Sulfolobaceae / genetics; Sulfolobaceae / metabolism
TL;DR:
Based on the highly conserved occurrence of single copies of MCE and MCM in Sulfolobaceae genomes, the M. sedula enzymes are likely to be representatives of these enzymes in the 3-HP/4-HB cycle in crenarchaeal thermoacidophiles.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018
2012 journal article
Extreme thermophiles: moving beyond single-enzyme biocatalysis
Current Opinion in Chemical Engineering, 1(4), 363–372.
TL;DR:
Although the pace for discovering novel microorganisms has slowed over the past two decades, genome sequence data have provided clues to novel biomolecules and metabolic pathways, which can be mined for a range of new applications.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
15. Life on Land
(Web of Science)
Sources: Crossref, NC State University Libraries
Added: February 24, 2020
2012 journal article
Hyperthermophilic Thermotoga Species Differ with Respect to Specific Carbohydrate Transporters and Glycoside Hydrolases
Applied and Environmental Microbiology, 78(6), 1978–1986.
MeSH headings : ATP-Binding Cassette Transporters / genetics; ATP-Binding Cassette Transporters / metabolism; Bacterial Typing Techniques; Carbohydrate Metabolism; Gene Expression Profiling; Glycoside Hydrolases / genetics; Glycoside Hydrolases / metabolism; Gram-Negative Anaerobic Straight, Curved, and Helical Rods / classification; Gram-Negative Anaerobic Straight, Curved, and Helical Rods / enzymology; Gram-Negative Anaerobic Straight, Curved, and Helical Rods / genetics; Gram-Negative Anaerobic Straight, Curved, and Helical Rods / metabolism; Microarray Analysis
TL;DR:
Results here show that subtle differences exist among the hyperthermophilic Thermotogales with respect to carbohydrate utilization, which supports their designation as separate species.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
15. Life on Land
(Web of Science)
Sources: Crossref, NC State University Libraries
Added: August 3, 2021
2012 journal article
S-Layer Homology Domain Proteins Csac_0678 and Csac_2722 Are Implicated in Plant Polysaccharide Deconstruction by the Extremely Thermophilic Bacterium Caldicellulosiruptor saccharolyticus
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 78(3), 768–777.
MeSH headings : Enzyme Stability; Glycoside Hydrolases / chemistry; Glycoside Hydrolases / genetics; Glycoside Hydrolases / metabolism; Gram-Positive Bacteria / enzymology; Gram-Positive Bacteria / genetics; Gram-Positive Bacteria / metabolism; Hydrogen-Ion Concentration; Membrane Glycoproteins / chemistry; Membrane Glycoproteins / genetics; Membrane Glycoproteins / metabolism; Molecular Weight; Open Reading Frames; Plants / microbiology; Polysaccharides / metabolism; Protein Binding; Sequence Deletion; Temperature
TL;DR:
Roles for Csac-0678 and Csac_2722 in recruitment and hydrolysis of complex polysaccharides and the deconstruction of lignocellulosic biomass are indicated and related SLH domain proteins in other Caldicellulosiruptor genomes may also be important contributors to plant biomass utilization.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
7. Affordable and Clean Energy
(Web of Science)
12. Responsible Consumption and Production
(Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018
2012 journal article
Uranium extremophily is an adaptive, rather than intrinsic, feature for extremely thermoacidophilic Metallosphaera species
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 109(41), 16702–16707.
author keywords: uranium oxidation; uranium resistance; chemolithotrophy; terminal oxidases
MeSH headings : Adaptation, Physiological / drug effects; Adaptation, Physiological / genetics; Archaeal Proteins / genetics; Archaeal Proteins / metabolism; Electrophoresis, Gel, Two-Dimensional; Environmental Pollutants / chemistry; Environmental Pollutants / toxicity; Gene Expression Regulation, Archaeal / drug effects; Mutation; RNA Stability / drug effects; RNA, Archaeal / genetics; RNA, Archaeal / metabolism; Species Specificity; Sulfolobaceae / classification; Sulfolobaceae / genetics; Sulfolobaceae / metabolism; Time Factors; Transcriptome / genetics; Uranium / chemistry; Uranium / toxicity
TL;DR:
The results point to uranium extremophily as an adaptive, rather than intrinsic, feature for Metallosphaera species, driven by environmental factors.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
12. Responsible Consumption and Production
(Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018
2011 journal article
A novel alpha-d-galactosynthase from Thermotoga maritima converts beta-d-galactopyranosyl azide to alpha-galacto-oligosaccharides
GLYCOBIOLOGY, 21(4), 448–456.
author keywords: alpha-galacto-oligosaccharides; glycosynthase; alpha-gal epitope; carbohydrate synthesis; protein engineering
MeSH headings : Aspartic Acid / chemistry; Azides / chemistry; Chromatography, Thin Layer; Disaccharides / biosynthesis; Galactose / metabolism; Glycine / chemistry; Kinetics; Mutagenesis, Site-Directed; Mutant Proteins / chemistry; Mutant Proteins / genetics; Mutant Proteins / metabolism; Protein Engineering; Thermotoga maritima / enzymology; alpha-Galactosidase / chemistry; alpha-Galactosidase / genetics; alpha-Galactosidase / metabolism
TL;DR:
Results open a new avenue to the practical synthesis of biologically interesting α-galacto-oligosaccharides and demonstrate more widespread use of β-glycosyl-azide as donors, confirming their utility to expand the repertoire of glycosynthases.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
7. Affordable and Clean Energy
(Web of Science)
12. Responsible Consumption and Production
(Web of Science)
14. Life Below Water
(OpenAlex)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018
2011 journal article
Complete Genome Sequences for the Anaerobic, Extremely Thermophilic Plant Biomass-Degrading Bacteria Caldicellulosiruptor hydrothermalis , Caldicellulosiruptor kristjanssonii , Caldicellulosiruptor kronotskyensis , Caldicellulosiruptor owensensis , and Caldicellulosiruptor lactoaceticus
Journal of Bacteriology, 193(6), 1483–1484.
MeSH headings : DNA, Bacterial / chemistry; DNA, Bacterial / genetics; Genetic Variation; Genome, Bacterial; Glycoside Hydrolases / genetics; Gram-Positive Bacteria / genetics; Membrane Transport Proteins / genetics; Molecular Sequence Data; Sequence Analysis, DNA
TL;DR:
Seven completed and one draft-phase Caldicellulosiruptor genomes suggest that, while central metabolism is highly conserved, significant differences in glycoside hydrolase inventories and numbers of carbohydrate transporters exist, a finding which likely relates to variability observed in plant biomass degradation capacity.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
7. Affordable and Clean Energy
(Web of Science)
12. Responsible Consumption and Production
(Web of Science)
Sources: Crossref, NC State University Libraries
Added: July 21, 2021
2011 journal article
Extremely Thermophilic Routes to Microbial Electrofuels
ACS CATALYSIS, 1(9), 1043–1050.
UN Sustainable Development Goal Categories
12. Responsible Consumption and Production
(Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018
2011 journal article
Glycoside Hydrolase Inventory Drives Plant Polysaccharide Deconstruction by the Extremely Thermophilic Bacterium Caldicellulosiruptor saccharolyticus
BIOTECHNOLOGY AND BIOENGINEERING, 108(7), 1559–1569.
author keywords: Caldicellulosiruptor saccharolyticus; extreme thermophile; plant biomass; glycoside hydrolases
MeSH headings : Bacterial Proteins / chemistry; Bacterial Proteins / genetics; Bacterial Proteins / metabolism; Carboxymethylcellulose Sodium / metabolism; Culture Media / chemistry; Gene Expression Profiling; Glycoside Hydrolases / chemistry; Glycoside Hydrolases / genetics; Glycoside Hydrolases / metabolism; Gram-Positive Bacteria / enzymology; Gram-Positive Bacteria / genetics; Gram-Positive Bacteria / metabolism; Molecular Weight; Plants / chemistry; Polysaccharides / metabolism; Xylans / metabolism
UN Sustainable Development Goal Categories
7. Affordable and Clean Energy
(Web of Science)
12. Responsible Consumption and Production
(Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018
2011 journal article
Insights into plant biomass conversion from the genome of the anaerobic thermophilic bacterium Caldicellulosiruptor bescii DSM 6725
NUCLEIC ACIDS RESEARCH, 39(8), 3240–3254.
MeSH headings : Bacterial Adhesion; Bacterial Proteins / genetics; Biomass; Carbohydrate Metabolism / genetics; Gene Expression Profiling; Genes, Bacterial; Genome, Bacterial; Genomics; Gram-Positive Bacteria / genetics; Gram-Positive Bacteria / metabolism; Gram-Positive Bacteria / ultrastructure; Plants / metabolism; Proteomics
TL;DR:
The high duplication of CAZy domains coupled with the ability to acquire foreign genes by LGT may have allowed the bacterium to rapidly adapt to changing plant biomass-rich environments.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
7. Affordable and Clean Energy
(Web of Science)
12. Responsible Consumption and Production
(Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018
2011 journal article
Starch Self-Processing in Transgenic Sweet Potato Roots Expressing a Hyperthermophilic alpha-Amylase
BIOTECHNOLOGY PROGRESS, 27(2), 351–359.
author keywords: hyperthermophilic enzymes; starch conversion; transgenic plants; sweet potato; biofuels
MeSH headings : Crops, Agricultural / genetics; Hot Temperature; Ipomoea batatas / genetics; Ipomoea batatas / metabolism; Plant Roots / metabolism; Plants, Genetically Modified / enzymology; Plants, Genetically Modified / metabolism; Southeastern United States; Starch / metabolism; Thermotoga maritima / enzymology; alpha-Amylases / genetics; alpha-Amylases / physiology
TL;DR:
The results presented here demonstrate that engineering plants with hyperthermophilic glycoside hydrolases can facilitate cost effective starch conversion to fermentable sugars and the use of sweet potato as an alternative near‐term energy crop should be considered.
(via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries, ORCID
Added: August 6, 2018
2011 journal article
VapC6, a ribonucleolytic toxin regulates thermophilicity in the crenarchaeote Sulfolobus solfataricus
RNA, 17(7), 1381–1392.
author keywords: antitoxin; RNA degradation; heat shock; archaea; transcription
MeSH headings : Adaptation, Physiological / genetics; Adaptation, Physiological / physiology; Cell Line; Gene Expression Profiling; Gene Expression Regulation, Archaeal; Hydrolysis / drug effects; Models, Biological; RNA / drug effects; RNA / metabolism; Sulfolobus solfataricus / genetics; Sulfolobus solfataricus / metabolism; Sulfolobus solfataricus / physiology; Temperature; Toxins, Biological / genetics; Toxins, Biological / metabolism; Toxins, Biological / pharmacology; Toxins, Biological / physiology
TL;DR:
The inactivation of the vapBC6 operon by targeted gene disruption produced two recessive phenotypes related to fitness, HS sensitivity and a heat-dependent reduction in the rate of growth that provide a biochemical mechanism and identify cellular targets underlying VapBC6-mediated control over microbial growth and survival at temperature extremes.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018
2010 chapter
Enzymes, Extremely Thermophilic
In M. C. Flickinger (Ed.), Encyclopedia of Industrial Biotechnology.
Ed(s): M. Flickinger
TL;DR:
The availability of enzymes with optimal functional temperatures above 70°C has had considerable impact on basic and applied elements of biocatalysis and there is still much to be learned to appreciate this complex biomolecular trait.
(via Semantic Scholar)
Sources: Crossref, NC State University Libraries
Added: August 3, 2021
2010 chapter
Extremely thermophilic microorganisms
In M. C. Flickinger (Ed.), Encyclopedia of Industrial Biotechnology: Bioprocess, Bioseparation, and Cell Technology (2nd ed., Vol. 5, pp. 3481–3499). NY: John Wiley and Sons.
Ed(s): M. Flickinger
Source: NC State University Libraries
Added: August 27, 2021
2010 entry reference
Extremophiles
(I. T. Horváth, Ed.).
Ed(s): I. Horváth
Sources: Crossref, NC State University Libraries, NC State University Libraries
Added: July 14, 2021
2010 journal article
Impact of Molecular Hydrogen on Chalcopyrite Bioleaching by the Extremely Thermoacidophilic Archaeon Metallosphaera sedula
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 76(8), 2668–2672.
MeSH headings : Archaeal Proteins / biosynthesis; Copper / metabolism; Electron Transport Chain Complex Proteins / biosynthesis; Gene Expression Profiling; Hydrogen / metabolism; Sulfolobaceae / metabolism
TL;DR:
Hydrogen served as a competitive inorganic energy source, impacting the CuFeS2 bioleaching efficiency of the extremely thermoacidophilic archaeon Metallosphaera sedula, and open reading frames encoding key terminal oxidase and electron transport chain components were triggered by Cu FeS2.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
7. Affordable and Clean Energy
(OpenAlex)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018
2010 journal article
N-terminal Fusion of a Hyperthermophilic Chitin-Binding Domain to Xylose Isomerase from Thermotoga neapolitana Enhances Kinetics and Thermostability of Both Free and Immobilized Enzymes
BIOTECHNOLOGY PROGRESS, 26(4), 993–1000.
author keywords: xylose isomerase; hyperthermophile; Thermotoga neapolitana; chitin-binding domain
MeSH headings : Aldose-Ketose Isomerases / genetics; Aldose-Ketose Isomerases / metabolism; Chitin / metabolism; Enzyme Stability; Enzymes, Immobilized / genetics; Enzymes, Immobilized / metabolism; Kinetics; Protein Binding; Protein Structure, Tertiary / genetics; Protein Structure, Tertiary / physiology; Recombinant Proteins / genetics; Recombinant Proteins / metabolism; Thermotoga neapolitana / enzymology
TL;DR:
Molecular modeling results suggest that the N‐terminal fusion impacted subunit interactions, thereby contributing to the enhanced thermostability of both the unbound and immobilized CBD‐TNXI, and played a role in modifying active site structure.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
12. Responsible Consumption and Production
(Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018
2010 journal article
Part I: characterization of the extracellular proteome of the extreme thermophile Caldicellulosiruptor saccharolyticus by GeLC-MS2
ANALYTICAL AND BIOANALYTICAL CHEMISTRY, 398(1), 377–389.
author keywords: Secretome; Caldicellulosiruptor saccharolyticus; GeLC-MS2; Thermophile; Sample preparation
MeSH headings : Bacterial Proteins / analysis; Bacterial Proteins / metabolism; Chromatography, Liquid; Electrophoresis, Polyacrylamide Gel; Extracellular Space / metabolism; Gram-Positive Bacteria / metabolism; Proteome / analysis; Spectrometry, Mass, Electrospray Ionization; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; Temperature
TL;DR:
In an effort to define the extracellular proteome of the extreme thermophilic bacterium Caldicellulosiruptor saccharolyticus, several techniques were considered regarding sample processing to achieve the most in-depth analysis of secreted proteins.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018
2010 article
Part I: characterization of the extracellular proteome of the extreme thermophile Caldicellulosiruptor saccharolyticus by GeLC-MS2 (vol 398, pg 377, 2010)
Andrews, G., Lewis, D., Notey, J., Kelly, R., & Muddiman, D. (2010, October). ANALYTICAL AND BIOANALYTICAL CHEMISTRY, Vol. 398, pp. 1837–1837.
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018
2010 journal article
Part II: defining and quantifying individual and co-cultured intracellular proteomes of two thermophilic microorganisms by GeLC-MS2 and spectral counting
ANALYTICAL AND BIOANALYTICAL CHEMISTRY, 398(1), 391–404.
author keywords: Thermotoga maritima; Caldicellulosiruptor saccharolyticus; GeLC-MS2; Co-cultivation; Intracellular proteome; Spectral counting
MeSH headings : Bacterial Proteins / analysis; Bacterial Proteins / metabolism; Chemotaxis; Chromatography, Liquid; Coculture Techniques; Electrophoresis, Polyacrylamide Gel; Fourier Analysis; Gram-Positive Bacteria / growth & development; Gram-Positive Bacteria / metabolism; Intracellular Space / metabolism; Proteome / analysis; Spectrometry, Mass, Electrospray Ionization; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; Spectrum Analysis / statistics & numerical data; Temperature; Thermotoga maritima / growth & development; Thermotoga maritima / metabolism
TL;DR:
Altering in growth conditions of these bacterial thermophilic microorganisms offer a glimpse into the intricacy of microbial behavior and interaction as well as insight into microbial interaction and behavior.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018
2010 article
Part II: defining and quantifying individual and co-cultured intracellular proteomes of two thermophilic microorganisms by GeLC-MS2 and spectral counting (vol 398, pg 391, 2010)
Andrews, G., Lewis, D., Notey, J., Kelly, R., & Muddiman, D. (2010, October). ANALYTICAL AND BIOANALYTICAL CHEMISTRY, Vol. 398, pp. 1839–1839.
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018
2010 journal article
Phylogenetic, Microbiological, and Glycoside Hydrolase Diversities within the Extremely Thermophilic, Plant Biomass-Degrading Genus Caldicellulosiruptor
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 76(24), 8084–8092.
MeSH headings : Bacterial Proteins / analysis; Biomass; Carbohydrate Metabolism; Cluster Analysis; DNA, Bacterial / chemistry; DNA, Bacterial / genetics; DNA, Ribosomal / chemistry; DNA, Ribosomal / genetics; Electrophoresis, Gel, Two-Dimensional; Genetic Variation; Glycoside Hydrolases / genetics; Gram-Positive Bacteria / classification; Gram-Positive Bacteria / genetics; Gram-Positive Bacteria / growth & development; Gram-Positive Bacteria / metabolism; Hot Temperature; Microarray Analysis; Molecular Sequence Data; Nucleic Acid Hybridization; Phylogeny; Plants / microbiology; Proteome / analysis; RNA, Ribosomal, 16S / genetics; Sequence Analysis, DNA
TL;DR:
Overall, the genus Caldicellulosiruptor appears to contain more genomic and physiological diversity than previously reported, and this argues for continued efforts to isolate new members from high-temperature terrestrial biotopes.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
7. Affordable and Clean Energy
(Web of Science)
12. Responsible Consumption and Production
(Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018
2010 journal article
Physiological Versatility of the Extremely Thermoacidophilic Archaeon Metallosphaera sedula Supported by Transcriptomic Analysis of Heterotrophic, Autotrophic, and Mixotrophic Growth
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 76(3), 931–935.
MeSH headings : Aerobiosis / genetics; Archaeal Proteins / genetics; Archaeal Proteins / metabolism; Autotrophic Processes; Carbon Dioxide / metabolism; Carbon Isotopes; Gene Expression Profiling; Gene Expression Regulation, Archaeal; Heterotrophic Processes; Hot Temperature; Hydroxybutyrates / metabolism; Oligonucleotide Array Sequence Analysis; Substrate Specificity / genetics; Sulfolobaceae / genetics; Sulfolobaceae / growth & development; Sulfolobaceae / metabolism; Temperature
TL;DR:
Comparative transcriptomic analysis of autotroph, heterotrophic, and mixotrophic growth of the archaeon Metallosphaera sedula revealed candidates for yet-to-be-confirmed components of the 3-hydroxypropionate/4-Hydroxybutyrate pathway and implicated a membrane-bound hydrogenase for growth on H2.
(via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018
2010 journal article
The genus Thermotoga: recent developments
ENVIRONMENTAL TECHNOLOGY, 31(10), 1169–1181.
author keywords: Thermotoga; extreme thermophiles; genomics; microbial physiology
MeSH headings : Biofilms; Carbohydrate Metabolism / genetics; Genome, Bacterial; Genomics; RNA, Ribosomal, 16S / genetics; Thermotoga maritima / enzymology; Thermotoga maritima / genetics; Thermotoga maritima / metabolism; Thermotoga maritima / physiology
TL;DR:
Recent developments in genome sequences have become available for a number of Thermotoga species, leading to functional genomics efforts to understand growth physiology as well as genomics‐based identification and characterization of novel high‐temperature biocatalysts.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
15. Life on Land
(Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018
2009 journal article
Carbohydrate Utilization Patterns for the Extremely Thermophilic Bacterium Caldicellulosiruptor saccharolyticus Reveal Broad Growth Substrate Preferences
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 75(24), 7718–7724.
MeSH headings : ATP-Binding Cassette Transporters / genetics; ATP-Binding Cassette Transporters / metabolism; Biofuels; Biological Transport; Carbohydrate Metabolism; Gene Expression Profiling; Genes, Bacterial; Gram-Positive Bacteria / genetics; Gram-Positive Bacteria / growth & development; Lignin / metabolism; Monosaccharides / metabolism; Oligonucleotide Array Sequence Analysis
TL;DR:
The results presented here affirm the broad growth substrate preferences of C. saccharolyticus on carbohydrates representative of lignocellulosic biomass and suggest that this bacterium holds promise for biofuel applications.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
7. Affordable and Clean Energy
(Web of Science)
12. Responsible Consumption and Production
(Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018
2009 journal article
Integrating modern biology into the ChE DNA through a campus-wide core laboratory education program
Chemical Engineering Education, 43(4), 257–264.
Source: NC State University Libraries
Added: September 6, 2021
2009 journal article
Plant cell calcium-rich environment enhances thermostability of recombinantly produced α-amylase from the hyperthermophilic bacterium Thermotoga maritime
Biotechnology and Bioengineering, 104(5), 947–956.
author keywords: hyperthermophilic enzymes; starch processing; transgenic plants; tobacco NT1 Cell cultures; biofuel
MeSH headings : Calcium / pharmacology; Coenzymes / pharmacology; Enzyme Stability; Escherichia coli / enzymology; Escherichia coli / genetics; Hot Temperature; Plants, Genetically Modified / enzymology; Recombinant Proteins / chemistry; Recombinant Proteins / genetics; Recombinant Proteins / metabolism; Thermotoga maritima / enzymology; Thermotoga maritima / genetics; Tobacco / enzymology; Tobacco / genetics; alpha-Amylases / chemistry; alpha-Amylases / genetics; alpha-Amylases / metabolism
TL;DR:
In a proof of concept study, tobacco cell cultures were used as model system to test in planta production of a hyperthermophilic α‐amylase from Thermotoga maritima, demonstrating the importance of a calcium‐rich environment for thermostability, as well as the advantage of producing this enzyme directly in plant cells where calcium is readily available.
(via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries, Crossref
Added: August 6, 2018
2009 journal article
Role of vapBC toxin–antitoxin loci in the thermal stress response of Sulfolobus solfataricus
Biochemical Society Transactions, 37(1), 123–126.
author keywords: archaeon; heat shock; hyperthermophile; stress response; Sulfolobus solfataricus; toxin-antioxin locus
MeSH headings : Antitoxins / metabolism; Archaeal Proteins / genetics; Archaeal Proteins / metabolism; Genome, Archaeal / genetics; Heat-Shock Response; Stress, Physiological; Sulfolobus solfataricus / genetics; Sulfolobus solfataricus / metabolism; Temperature; Toxins, Biological / genetics; Toxins, Biological / metabolism
TL;DR:
Findings indicate that more work needs to be done to determine the role of VapBCs in S. solfataricus and other thermophilic archaea, especially with respect to post-transcriptional regulation.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Crossref, NC State University Libraries
Added: August 28, 2020
2009 journal article
Temperature, not LuxS, mediates AI-2 formation in hydrothermal habitats
FEMS MICROBIOLOGY ECOLOGY, 68(2), 173–181.
author keywords: autoinducer-2; AI-2; hyperthermophiles; hydrothermal environments; Thermotoga maritima; Pyrococcus furiosus
MeSH headings : Bacterial Proteins / genetics; Bacterial Proteins / metabolism; Carbon-Sulfur Lyases / genetics; Carbon-Sulfur Lyases / metabolism; Gas Chromatography-Mass Spectrometry; Gene Expression Regulation, Bacterial; Homoserine / analogs & derivatives; Homoserine / biosynthesis; Hot Temperature; Lactones; Pyrococcus furiosus / genetics; Pyrococcus furiosus / metabolism; Quorum Sensing; Thermotoga maritima / genetics; Thermotoga maritima / metabolism
TL;DR:
Autoinducer-2 (AI-2), a boronated furanone and proposed 'universal' interspecies mesophilic bacterial communication signal, could be formed by Thermotoga maritima and Pyrococcus furiosus through a combination of biotic and abiotic reaction steps, but did not induce any detectable quorum-sensing phenotypes in these organisms.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
14. Life Below Water
(OpenAlex)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018
2008 review
Extremely thermophilic microorganisms for biomass conversion: status and prospects
[Review of ]. CURRENT OPINION IN BIOTECHNOLOGY, 19(3), 210–217.
MeSH headings : Archaea / classification; Archaea / genetics; Archaea / metabolism; Bacteria / classification; Bacteria / genetics; Bacteria / metabolism; Bioelectric Energy Sources; Biomass; Biotechnology / trends; Carbohydrate Metabolism; Cellulase / metabolism; Cellulose / metabolism; Energy-Generating Resources; Hot Temperature; Marine Biology; Phylogeny
TL;DR:
Characterization of extremely thermophilic enzymes implicated in the deconstruction of lignocellulose can be identified in genome sequences, and many more promising biocatalysts probably remain annotated as 'hypothetical proteins'.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
7. Affordable and Clean Energy
(Web of Science)
12. Responsible Consumption and Production
(Web of Science)
14. Life Below Water
(OpenAlex)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018
2008 chapter
Functional Genomics of Stress Response in Extremophilic Archaea
In P. Blum (Ed.), Archaea: New Models for Prokaryotic Biology. Norfolk, UK: Caister Academic Press.
Ed(s): P. Blum
Source: NC State University Libraries
Added: September 9, 2021
2008 journal article
Functional-genomics-based identification and characterization of open reading frames encoding alpha-glucoside-processing enzymes in the hyperthermophilic archaeon Pyrococcus furiosus
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 74(4), 1281–1283.
MeSH headings : Computational Biology; Gene Expression Regulation, Bacterial / genetics; Genomics / methods; Open Reading Frames / genetics; Pyrococcus furiosus / enzymology; Pyrococcus furiosus / genetics; alpha-Glucosidases / genetics; beta-Amylase / genetics
TL;DR:
Bioinformatics analysis and transcriptional response information for Pyrococcus furiosus led to the identification of a novel isomaltase representing a new glycoside hydrolase family, a novel GH57 β-amylase, and an extracellular starch-binding protein.
(via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018
2008 review
Hydrogenesis in hyperthermophilic microorganisms: Implications for biofuels
[Review of ]. METABOLIC ENGINEERING, 10(6), 394–404.
author keywords: Biohydrogen; Pyrococcus furiosus; Thermotoga maritima; Caldicellulosiruptor saccharolyticus; Hyperthermophiles; Hydrogenases
MeSH headings : Archaea / metabolism; Bioelectric Energy Sources; Hot Temperature; Hydrogen / metabolism; Hydrogen Sulfide / metabolism; Methane / metabolism
TL;DR:
To fully exploit the use of these novel microorganisms and their constituent hydrogenases for biohydrogen production, development of versatile genetic systems and improvements in current understanding of electron flux from fermentable substrates to H(2) in hyperthermophiles are needed.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
12. Responsible Consumption and Production
(Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018
2008 journal article
Hydrogenomics of the Extremely Thermophilic Bacterium Caldicellulosiruptor saccharolyticus
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 74(21), 6720–6729.
MeSH headings : Bacterial Proteins / genetics; Carbohydrate Metabolism / genetics; DNA, Bacterial / chemistry; DNA, Bacterial / genetics; Enzymes / genetics; Gene Expression Profiling; Genome, Bacterial; Gram-Positive Bacteria / genetics; Metabolic Networks and Pathways / genetics; Molecular Sequence Data; Sequence Analysis, DNA
UN Sustainable Development Goal Categories
7. Affordable and Clean Energy
(Web of Science)
12. Responsible Consumption and Production
(Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018
2008 journal article
Identification of Components of Electron Transport Chains in the Extremely Thermoacidophilic Crenarchaeon Metallosphaera sedula through Iron and Sulfur Compound Oxidation Transcriptomes
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 74(24), 7723–7732.
MeSH headings : Archaeal Proteins / biosynthesis; Archaeal Proteins / genetics; Electron Transport Chain Complex Proteins / biosynthesis; Electron Transport Chain Complex Proteins / genetics; Ferrous Compounds / metabolism; Gene Expression Profiling; Genes, Archaeal; Iron-Sulfur Proteins / biosynthesis; Iron-Sulfur Proteins / genetics; Multigene Family; Oxidation-Reduction; Oxidoreductases / biosynthesis; Oxidoreductases / genetics; Sulfolobaceae / enzymology; Sulfolobaceae / genetics; Sulfolobaceae / metabolism; Sulfur / metabolism
TL;DR:
From this analysis, a comprehensive model for electron transport in M. sedula could be proposed as the basis for examining specific details of iron and sulfur oxidation in this bioleaching archaeon.
(via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018
2008 review
Life in hot acid: Pathway analyses in extremely thermoacidophilic archaea
[Review of ]. CURRENT OPINION IN BIOTECHNOLOGY, 19(5), 445–453.
MeSH headings : Acids / metabolism; Archaea / physiology; Archaeal Proteins / metabolism; Cell Survival; Ecosystem; Hot Temperature; Hydrogen-Ion Concentration; Models, Biological; Signal Transduction / physiology
TL;DR:
New insights into the metabolic pathways and physiological features that define extreme thermoacidophily have been obtained, in some cases suggesting prospects for biotechnological opportunities.
(via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018
2008 journal article
Microwave activation of enzymatic catalysis
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 130(31), 10048-+.
MeSH headings : Carboxylesterase / metabolism; Catalysis; Enzymes / metabolism; Hot Temperature; Kinetics; Microwaves; Pyrococcus furiosus / enzymology; Sulfolobus solfataricus / enzymology; Thermotoga maritima / enzymology; alpha-Galactosidase / metabolism; beta-Glucosidase / metabolism
TL;DR:
This is the first illustration of a specific microwave effect in enzymatic catalysis, used to regulate biocatalysis, in a microwave reactor.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
6. Clean Water and Sanitation
(OpenAlex)
12. Responsible Consumption and Production
(Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018
2008 journal article
Polysaccharide Degradation and Synthesis by Extremely Thermophilic Anaerobes
Annals of the New York Academy of Sciences, 1125(1), 322–337.
author keywords: Pyrococcus furiosus; Thermotoga maritima; Caldicellulosiruptor saccharolyticus; extremely thermophilic organism; thermophile; glycoside hydrolases; exopolysaccharides
MeSH headings : Anaerobiosis; Archaeal Proteins / metabolism; Bacterial Proteins / metabolism; Biological Transport; Carbohydrate Metabolism; Glycoside Hydrolases / metabolism; Hot Temperature; Polysaccharides / biosynthesis; Polysaccharides / metabolism; Pyrococcus furiosus / growth & development; Pyrococcus furiosus / metabolism; Thermotoga maritima / growth & development; Thermotoga maritima / metabolism
TL;DR:
The genomes of three model extreme thermophiles and two bacteria, Thermotoga maritima and Caldicellulosiruptor saccharolyticus,encode numerous carbohydrate‐active enzymes, many of which have been characterized biochemically in their native or recombinant forms.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
7. Affordable and Clean Energy
(Web of Science)
12. Responsible Consumption and Production
(Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018
2008 journal article
Probing the stability of native and activated forms of alpha(2)-macroglobulin
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES, 42(1), 62–67.
author keywords: alpha M-2; alpha(2)-macroglobulin; alpha(2)-macroglobulin and proteinase incorporation; alpha(2)-macroglobulin and norproteolytic incorporation of lysozyme; alpha(2)-macroglobulin stabilization
MeSH headings : Amino Acid Sequence; Animals; Hot Temperature; Humans; Ligands; Molecular Sequence Data; Peptide Hydrolases / chemistry; Protein Conformation; Transition Temperature; alpha-Macroglobulins / chemistry
TL;DR:
Differential scanning calorimetry and urea gel electrophoresis demonstrate that both bait region cleavage and covalent incorporation of protein ligands in the thioester pocket play critical roles in the stability of α 2 M complexes.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018
2008 journal article
The genome sequence of the metal-mobilizing, extremely thermoacidophilic archaeon Metallosphaera sedula provides insights into bioleaching-associated metabolism
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 74(3), 682–692.
MeSH headings : Amino Acid Sequence; Archaeal Proteins / genetics; Archaeal Proteins / metabolism; Gene Expression Regulation, Archaeal; Genome, Archaeal; Hot Temperature; Iron / metabolism; Molecular Sequence Data; Oligonucleotide Array Sequence Analysis; Oxidation-Reduction; Sequence Analysis, DNA; Sulfates / metabolism; Sulfolobaceae / classification; Sulfolobaceae / genetics; Sulfolobaceae / metabolism
TL;DR:
Comparisons with the mesophilic biomining bacterium Acidithiobacillus ferrooxidans indicate that the M. sedula genome encodes at least one putative rusticyanin, involved in iron oxidation, and a putative tetrathionate hydrolase, implicated in sulfur oxidation, which is a desirable trait of biomining microorganisms.
(via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018
2007 chapter
Archaeal 20S Proteasome: A Simple and Thermostable Model System for the Core Particle
In F. Robb, G. Antranikian, D. Grogan, & A. Driessen (Eds.), Thermophiles (pp. 347–360).
Ed(s): F. Robb, G. Antranikian, D. Grogan & A. Driessen
Sources: Crossref, NC State University Libraries
Added: August 3, 2021
2007 journal article
Biochemical analysis of Thermotoga maritima GH36 alpha-galactosidase (TmGalA) confirms the mechanistic commonality of clan GH-D glycoside hydrolases
BIOCHEMISTRY, 46(11), 3319–3330.
MeSH headings : Amino Acid Sequence; Azides / pharmacology; Catalysis; Cloning, Molecular; Hot Temperature; Hydrogen-Ion Concentration; Kinetics; Models, Molecular; Molecular Sequence Data; Mutagenesis, Site-Directed; Protein Denaturation; Sequence Alignment; Substrate Specificity; Thermotoga maritima / enzymology; Thermotoga maritima / genetics; alpha-Galactosidase / drug effects; alpha-Galactosidase / genetics; alpha-Galactosidase / metabolism
TL;DR:
Results show that the biochemical characteristics of GH36 TmGalA are closely related to GH27 enzymes, confirming the mechanistic commonality of clan GH-D members, and confirm that Asp387 is the acid/base residue of Tm GalA.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger
(Web of Science)
14. Life Below Water
(OpenAlex)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018
2007 chapter
Biochemical/Biomolecular engineering
In McGraw-Hill encyclopedia of science & technology (10th ed.). New York: McGraw-Hill.
Source: NC State University Libraries
Added: September 9, 2021
2007 chapter
Functional Genomics
In R. Cavicchioli (Ed.), Archaea: Molecular and Cellular Biology (pp. 434–462).
Ed(s): R. Cavicchioli
Sources: NC State University Libraries, NC State University Libraries
Added: July 27, 2021
2007 journal article
Impact of substrate glycoside linkage. and elemental sulfur on bioenergetics, of and hydrogen production by the hyperthermophilic Archaeon Pyrococcus furiosus
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 73(21), 6842–6853.
MeSH headings : Archaea; Chemical Phenomena; Chemistry; Gene Expression Regulation, Archaeal; Genome, Archaeal; Glycosides / metabolism; Hot Temperature; Hydrogen / metabolism; Oligonucleotide Array Sequence Analysis; Open Reading Frames; Pyrococcus furiosus / enzymology; Pyrococcus furiosus / genetics; Pyrococcus furiosus / physiology; Sulfur / metabolism
UN Sustainable Development Goal Categories
7. Affordable and Clean Energy
(Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018
2007 journal article
Responses of wild-type and resistant strains of the hyperthermophilic bacterium Thermotoga maritima to chloramphenicol challenge
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 73(15), 5058–5065.
MeSH headings : Anti-Bacterial Agents / pharmacology; Bacterial Proteins / genetics; Bacterial Proteins / metabolism; Base Sequence; Chloramphenicol / pharmacology; Drug Resistance, Bacterial / genetics; Gene Expression Profiling; Gene Expression Regulation, Bacterial; Hot Temperature; Microbial Sensitivity Tests; Molecular Sequence Data; Oligonucleotide Array Sequence Analysis; Point Mutation; Proteome; RNA, Ribosomal, 23S / genetics; Thermotoga maritima / drug effects; Thermotoga maritima / genetics; Thermotoga maritima / growth & development; Transcription, Genetic
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
14. Life Below Water
(OpenAlex)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018
2007 journal article
Rheological properties of guar galactomannan solutions during hydrolysis with galactomannanase and alpha-galactosidase enzyme mixtures
BIOMACROMOLECULES, 8(3), 949–956.
MeSH headings : Carbohydrate Conformation; Galactans / metabolism; Galactose / analogs & derivatives; Hydrogen-Ion Concentration; Hydrolysis; Kinetics; Mannans / chemistry; Mannans / metabolism; Mannose / chemistry; Mannosidases / chemistry; Models, Chemical; Models, Statistical; Plant Gums / metabolism; Polysaccharides / chemistry; Rheology / methods; Time Factors; alpha-Galactosidase / chemistry
TL;DR:
A rheology-based kinetic model is developed to estimate the reaction rate constants and interpret synergistic effects of multiple enzyme contributions and reveals that both the native and the debranched guar have the same activation energy for beta-mannanase action, although debranching considerably increases the frequency of enzyme-guar interactions.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018
2007 journal article
Role of the beta 1 subunit in the function and stability of the 20S proteasome in the hyperthermophilic archaeon Pyrococcus furiosus
JOURNAL OF BACTERIOLOGY, 189(2), 583–590.
MeSH headings : Archaeal Proteins / genetics; Archaeal Proteins / metabolism; Archaeal Proteins / physiology; Calorimetry, Differential Scanning; Genome, Archaeal; Hot Temperature; Molecular Chaperones / genetics; Molecular Chaperones / metabolism; Proteasome Endopeptidase Complex / genetics; Proteasome Endopeptidase Complex / metabolism; Proteasome Endopeptidase Complex / physiology; Protein Subunits / genetics; Protein Subunits / metabolism; Protein Subunits / physiology; Pyrococcus furiosus / genetics; Pyrococcus furiosus / metabolism; Temperature; Transcription, Genetic
TL;DR:
Results indicate that the β1 subunit in the P. furiosus 20S proteasome plays a thermostabilizing role and influences biocatalytic properties, suggesting that β subunit composition is a factor in archaeal proteasom function during thermal stress, when polypeptide turnover is essential to cell survival.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018
2007 chapter
Transcriptomics, Proteomics, and Structural Genomics ofPyrococcus Furiosus
In Archaea (pp. 239–246).
Sources: Crossref, NC State University Libraries
Added: July 21, 2021
2006 journal article
Colocation of genes encoding a tRNA-mRNA hybrid and a putative signaling peptide on complementary strands in the genome of the hyperthermophilic bacterium Thermotoga maritima
JOURNAL OF BACTERIOLOGY, 188(19), 6802–6807.
MeSH headings : Amino Acid Sequence; Anti-Bacterial Agents / pharmacology; Chloramphenicol / pharmacology; Coculture Techniques; DNA, Bacterial / genetics; Gene Expression; Gene Expression Regulation, Bacterial; Genes, Bacterial; Genome, Bacterial; Intracellular Signaling Peptides and Proteins / genetics; Methanococcus; RNA, Bacterial / analysis; RNA, Bacterial / genetics; RNA, Messenger / analysis; RNA, Messenger / genetics; Reverse Transcriptase Polymerase Chain Reaction; Sequence Alignment; Synteny; Thermotoga maritima / genetics; Transcription, Genetic
TL;DR:
Specific DNA probes were designed and used in real-time PCR assays to follow the separate transcriptional responses of the colocated open reading frames (ORFs) during transition from exponential to stationary phase, chloramphenicol challenge, and syntrophic coculture with Methanococcus jannaschii.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
14. Life Below Water
(OpenAlex)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018
2006 journal article
Dynamic metabolic adjustments and genome plasticity are implicated in the heat shock response of the extremely thermoacidophilic Archaeon Sulfolobus solfataricus
JOURNAL OF BACTERIOLOGY, 188(12), 4553–4559.
MeSH headings : Antitoxins / genetics; Antitoxins / metabolism; Archaeal Proteins / genetics; Archaeal Proteins / metabolism; Heat-Shock Response; Hot Temperature; Hydrogen-Ion Concentration; RNA, Archaeal / genetics; RNA, Messenger / genetics; Sulfolobus solfataricus / metabolism; Sulfolobus solfataricus / physiology; Time Factors; Toxins, Biological / genetics; Toxins, Biological / metabolism
TL;DR:
Approximately one-third of the open reading frames encoded in the Sulfolobus solfataricus genome were differentially expressed within 5 min following an 80 to 90 degrees C temperature shift at pH 4.0, implicating a connection between genome plasticity and metabolic regulation in the early stages of stress response.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018
2006 journal article
Global analysis of carbohydrate utilization by Lactobacillus acidophilus using cDNA microarrays
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 103(10), 3816–3821.
author keywords: ATP-binding cassette; carbon catabolite repression; fructooligosaccharide; galactoside-pentose hexuronide
MeSH headings : ATP-Binding Cassette Transporters / genetics; ATP-Binding Cassette Transporters / metabolism; Bacterial Proteins / genetics; Bacterial Proteins / metabolism; Carbohydrate Metabolism / genetics; Gastrointestinal Tract / microbiology; Gene Expression Profiling; Genes, Bacterial; Humans; Lactobacillus acidophilus / genetics; Lactobacillus acidophilus / metabolism; Models, Biological; Oligonucleotide Array Sequence Analysis; Phosphoenolpyruvate Sugar Phosphotransferase System / genetics; Reverse Transcriptase Polymerase Chain Reaction
TL;DR:
The transport and catabolic machinery involved in carbohydrate utilization by Lactobacillus acidophilus was characterized genetically by using whole-genome cDNA microarrays, and genes central to glycolysis were among the most highly expressed in the genome.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, NC State University Libraries, ORCID
Added: August 6, 2018
2006 journal article
Identification of Eggshell Membrane Proteins and Purification of Ovotransferrin and β-NAGase from Hen Egg White
The Protein Journal, 25(1), 71–81.
author keywords: beta-N-acetylglucosaminidase; ovotransferrin; isoelectric focusing; SDS-PAGE; eggshell membrane; hen egg white
MeSH headings : Acetylglucosaminidase / analysis; Acetylglucosaminidase / isolation & purification; Animals; Anti-Bacterial Agents / analysis; Chickens; Chromatography, Ion Exchange; Conalbumin / analysis; Conalbumin / isolation & purification; Egg Proteins / chemistry; Electrophoresis, Polyacrylamide Gel; Isoelectric Focusing; Membrane Proteins / chemistry; Muramidase / analysis; Ovum / chemistry; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
TL;DR:
Attempts to purify active fractions of β-N-acetylglucosaminidase, lysozyme and ovotransferrin from the ESM proved somewhat limited and will be required in order to fully elucidate the mechanism responsible for the antimicrobial properties associated with the E SM.
(via Semantic Scholar)
Sources: Crossref, NC State University Libraries, Web Of Science
Added: August 6, 2018
2006 review
Microbial biochemistry, physiology, and biotechnology of hyperthermophilic Thermotoga species
[Review of ]. FEMS MICROBIOLOGY REVIEWS, 30(6), 872–905.
author keywords: Thermotoga; hyperthermophiles; functional genomics
MeSH headings : Bacterial Proteins / chemistry; Bacterial Proteins / metabolism; Bacterial Proteins / physiology; Biotechnology; Carbohydrate Metabolism; Genetic Variation; Genome, Bacterial; Genomics; Gram-Negative Anaerobic Straight, Curved, and Helical Rods / genetics; Gram-Negative Anaerobic Straight, Curved, and Helical Rods / physiology; Hot Temperature; Iron / metabolism; Monosaccharides / biosynthesis; Phylogeny; Polysaccharides / biosynthesis; Signal Transduction; Sulfur / metabolism; Thermotoga maritima / genetics; Thermotoga maritima / isolation & purification; Thermotoga maritima / physiology
TL;DR:
Structural genomics efforts aimed at the model hyperthermophile Thermotoga maritima proteome have yielded hundreds of high-resolution datasets and predicted functions for uncharacterized proteins, particularly for carbohydrate utilization and iron-sulfur cluster synthesis and repair.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
12. Responsible Consumption and Production
(Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018
2006 journal article
The Thermotoga maritima phenotype is impacted by syntrophic interaction with Methanococcus jannaschii in hyperthermophilic coculture
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 72(1), 811–818.
MeSH headings : Bacterial Proteins / genetics; Bacterial Proteins / metabolism; Coculture Techniques; DNA, Complementary; Gene Expression Profiling; Gene Expression Regulation, Bacterial; Hot Temperature; Methanococcus / growth & development; Oligonucleotide Array Sequence Analysis; Phenotype; Proteome; Thermotoga maritima / classification; Thermotoga maritima / genetics; Thermotoga maritima / growth & development; Thermotoga maritima / metabolism; Transcription, Genetic
TL;DR:
The results demonstrate that syntrophic interactions can impact the transcriptome of heterotrophs in methanogenic coculture, and this factor should be considered in examining the microbial ecology in anaerobic environments.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
14. Life Below Water
(OpenAlex)
15. Life on Land
(Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018
2006 journal article
Transcriptional and biochemical analysis of starch metabolism in the hyperthermophilic archaeon Pyrococcus furiosus
JOURNAL OF BACTERIOLOGY, 188(6), 2115–2125.
MeSH headings : Archaeal Proteins / genetics; Bacterial Proteins / genetics; DNA, Archaeal / chemistry; DNA, Archaeal / genetics; Gene Expression Profiling; Gene Expression Regulation, Archaeal; Glucose / metabolism; Glucosephosphates / metabolism; Glucosyltransferases / genetics; Glycogen Debranching Enzyme System / genetics; Glycoside Hydrolases / genetics; Monosaccharide Transport Proteins / genetics; Oligonucleotide Array Sequence Analysis; Pyrococcus furiosus / genetics; Pyrococcus furiosus / metabolism; Sequence Analysis, DNA; Starch / metabolism; Substrate Specificity
TL;DR:
Starch metabolism in P. furiosus was examined by biochemical analyses in conjunction with global transcriptional response data for cells grown on a variety of glucans, and several hypothetical proteins encoded by three gene clusters are also involved in the transport and processing of alpha-glucan substrates by this organism.
(via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018
2005 journal article
Aflatoxin conducive and non-conducive growth conditions reveal new gene associations with aflatoxin production
FUNGAL GENETICS AND BIOLOGY, 42(6), 506–518.
author keywords: aflatoxin; gene expression; regulation; Aspergillus flavus; Aspergillus parasiticus
MeSH headings : Aflatoxins / biosynthesis; Aspergillus / genetics; Aspergillus / metabolism; Gene Expression; Gene Expression Profiling; Gene Expression Regulation, Fungal; Genes, Fungal; Hydrogen-Ion Concentration; Nitrogen / metabolism; Oligonucleotide Array Sequence Analysis; RNA, Fungal / analysis; RNA, Messenger / analysis; Regulon; Temperature
TL;DR:
To understand the effect of nitrogen source, carbon source, temperature, and pH on the regulation of AF biosynthesis, a targeted cDNA microarray consisting of genes associated with AF production over time was employed.
(via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018
2005 journal article
An expression-driven approach to the prediction of carbohydrate transport and utilization regulons in the hyperthermophilic bacterium Thermotoga maritima
JOURNAL OF BACTERIOLOGY, 187(21), 7267–7282.
MeSH headings : 5' Flanking Region / genetics; ATP-Binding Cassette Transporters / chemistry; ATP-Binding Cassette Transporters / genetics; Biological Evolution; Biological Transport / genetics; Carbohydrate Metabolism; Computational Biology; Gene Duplication; Gene Expression Profiling; Genes, Bacterial; Genes, Regulator; Genomics; Monosaccharide Transport Proteins / genetics; Nucleic Acid Conformation; Oligosaccharides / metabolism; Pentose Phosphate Pathway / genetics; Regulon / physiology; Rhamnose / metabolism; Thermotoga maritima / genetics; Thermotoga maritima / metabolism; Xylose / metabolism
TL;DR:
The results indicate that T. maritima relies extensively on ABC transporters for carbohydrate uptake, many of which are likely controlled by local regulators responsive to either the transport substrate or a key metabolic degradation product.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
7. Affordable and Clean Energy
(Web of Science)
12. Responsible Consumption and Production
(Web of Science)
14. Life Below Water
(OpenAlex)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018
2005 patent
Compositions for fracturing subterranean formations
Washington, DC: U.S. Patent and Trademark Office.
Source: NC State University Libraries
Added: August 6, 2018
2005 journal article
Denaturation and aggregation of three alpha-lactalbumin preparations at neutral pH
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 53(8), 3182–3190.
author keywords: alpha-lactalbumin; whey proteins; denaturation and aggregation
MeSH headings : Calorimetry, Differential Scanning; Chemical Phenomena; Chemistry, Physical; Dimerization; Electrophoresis, Polyacrylamide Gel; Hot Temperature; Hydrogen-Ion Concentration; Lactalbumin / chemistry; Nephelometry and Turbidimetry; Protein Denaturation
TL;DR:
The denaturation and aggregation of reagent-grade, ion-exchange chromatography purified, and a commercial-grade alpha-lactalbumin were studied and the interrelationship between specific electrostatic interactions and hydrophobic attraction, in relation to the formation of disulfide-bonded products, is discussed.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018
2005 journal article
Genorne-wide transcriptional variation within and between steady states for continuous growth of the hyperthermophile Thermotoga maritima
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 71(9), 5572–5576.
TL;DR:
Maltose-limited, continuous growth of the hyperthermophile Thermotoga maritima at different temperatures and dilution rates showed that transcriptome-wide variation in gene expression within mechanical steady states was minimal compared to that between steady states, supporting the efficacy of chemostat-based approaches for functional genomics studies.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
14. Life Below Water
(OpenAlex)
15. Life on Land
(Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018
2005 journal article
Influence of divalent cations on the structural thermostability and thermal inactivation kinetics of class II xylose isomerases
FEBS JOURNAL, 272(6), 1454–1464.
author keywords: inactivation kinetics; metal cofactors; thermostability; xylose isomerases
MeSH headings : Aldose-Ketose Isomerases / antagonists & inhibitors; Aldose-Ketose Isomerases / chemistry; Aldose-Ketose Isomerases / metabolism; Bacillus / enzymology; Bacterial Proteins / antagonists & inhibitors; Bacterial Proteins / chemistry; Bacterial Proteins / metabolism; Cations, Divalent / pharmacology; Cobalt / pharmacology; Enzyme Stability; Escherichia coli / enzymology; Kinetics; Magnesium / pharmacology; Manganese / pharmacology; Protein Denaturation; Recombinant Proteins / antagonists & inhibitors; Recombinant Proteins / chemistry; Recombinant Proteins / metabolism; Thermodynamics; Thermotoga maritima / enzymology
TL;DR:
The results reported here suggest that, unlike the other class II XIs examined, single metals are required for TNXI activity, but are not essential for its structural thermostability.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
12. Responsible Consumption and Production
(Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018
2005 journal article
Population density-dependent regulation of exopolysaccharide formation in the hyperthermophilic bacterium Thermotoga maritima
MOLECULAR MICROBIOLOGY, 55(3), 664–674.
MeSH headings : Bacterial Proteins / genetics; Bacterial Proteins / metabolism; Culture Media; Ecosystem; Gene Expression Profiling; Gene Expression Regulation, Bacterial; Genome, Archaeal; Genome, Bacterial; Methanococcus / genetics; Methanococcus / growth & development; Methanococcus / metabolism; Oligonucleotide Array Sequence Analysis; Polysaccharides, Bacterial / metabolism; Signal Transduction; Temperature; Thermotoga maritima / growth & development; Thermotoga maritima / metabolism; Transcription, Genetic
TL;DR:
Results point to the existence of peptide‐based quorum sensing in hyperthermophilic bacteria and indicate that cellular communication should be considered as a component of the microbial ecology within hydrothermal habitats.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
14. Life Below Water
(OpenAlex)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018
2004 chapter
Applications of Genomic Data: Enzyme Discovery and Microbial Genomics
In C. M. Fraser, T. Read, & K. E. Nelson (Eds.), Microbial Genomics. Totowa, NJ: Humana Press, Inc.
Ed(s): C. Fraser, T. Read & K. Nelson
Source: NC State University Libraries
Added: September 11, 2021
2004 chapter
Expression of Extremophilic Proteins
In F. Baneyx (Ed.), Expression Technologies: Current Status and Future Trends. Horizon Scientific.
Ed(s): F. Baneyx
Source: NC State University Libraries
Added: September 11, 2021
2004 article
Functional genomics-based studies of the microbial ecology of hyperthermophilic micro-organisms
Johnson, M. R., Montero, C. I., Conners, S. B., Shockley, K. R., Pysz, M. A., & Kelly, R. M. (2004, April). BIOCHEMICAL SOCIETY TRANSACTIONS, Vol. 32, pp. 188–192.
author keywords: ecology; functional qenomics; hyperthermophile; microarray; mixed culture
MeSH headings : Biofilms; DNA, Complementary / metabolism; Ecology; Gene Transfer Techniques; Genes, Archaeal; Genome; Genome, Archaeal; Microscopy, Fluorescence; Oligonucleotide Array Sequence Analysis; Phylogeny; RNA, Ribosomal, 16S / genetics; Temperature
TL;DR:
Functional genomics-based approaches are described, using cDNA microarrays, to gain insight into how ecological features such as biofilm formation, species interaction, and possibly even gene transfer may occur in native environments, as well as to determine what genes or sets of genes may be tied to environmental functionality.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
15. Life on Land
(Web of Science; OpenAlex)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018
2004 chapter
Pyrococcus furiosus Protease I (PfpI)
In F. Woessner, N. Rawlings, & A. Barrett (Eds.), Handbook of Proteolytic Enzymes (pp. 1393–1395). London: Academic Press Limited.
Ed(s): F. Woessner, N. Rawlings & A. Barrett
Source: NC State University Libraries
Added: September 11, 2021
2004 chapter
Significance of polysaccharides in microbial physiology and the ecology of hydrothermal vent environments
In W. S. D. Wilcock, E. F. DeLong, D. S. Kelley, J. A. Baross, & S. C. Cary (Eds.), The Subseafloor Biosphere at Mid-Ocean Ridges (pp. 213–226). Washington, DC: American Geophysical Union.
Ed(s): W. Wilcock, E. DeLong, D. Kelley, J. Baross & S. Cary
Source: NC State University Libraries
Added: September 9, 2021
2004 journal article
Strategic biocatalysis with hyperthermophilic enzymes
Green Chemistry, 6(9), 459.
TL;DR:
Examples of several possible ways in which hyperthermophilic enzymes could be used are presented, including cases where they could serve as environmentally benign alternatives in existing industrial processes.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
7. Affordable and Clean Energy
(Web of Science)
12. Responsible Consumption and Production
(Web of Science)
Sources: Crossref, NC State University Libraries
Added: February 24, 2020
2004 review
Transcriptional analysis of biofilm formation processes in the anaerobic, hyperthermophilic bacterium Thermotoga maritima
[Review of ]. APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 70(10), 6098–6112.
MeSH headings : ATP-Binding Cassette Transporters / genetics; ATP-Binding Cassette Transporters / metabolism; Base Sequence; Biofilms / growth & development; Bioreactors; DNA, Bacterial / genetics; Gene Expression Profiling; Genes, Bacterial; Iron / metabolism; Models, Biological; Oligonucleotide Array Sequence Analysis; Oxidative Stress; Polysaccharides, Bacterial / genetics; Polysaccharides, Bacterial / metabolism; Sulfur / metabolism; Temperature; Thermotoga maritima / genetics; Thermotoga maritima / physiology; Thermotoga maritima / ultrastructure; Transcription, Genetic
TL;DR:
In addition to revealing insights into the phenotype of sessile T. maritima communities, the methodology developed here can be extended to study other anaerobic biofilm formation processes as well as to examine aspects of microbial ecology in hydrothermal environments.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
14. Life Below Water
(OpenAlex)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018
2004 journal article
Transcriptional analysis of dynamic heat-shock response by the hyperthermophilic bacterium Thermotoga maritima
EXTREMOPHILES, 8(3), 209–217.
author keywords: cDNA microarray; groESL; heat shock; hrcA; hyperthermophile; Thermotoga
MeSH headings : Base Sequence; DNA, Bacterial / genetics; Endopeptidases / genetics; Gene Expression; Genome, Bacterial; Heat-Shock Response / genetics; Hot Temperature; Oligonucleotide Array Sequence Analysis; SOS Response, Genetics; Sigma Factor / genetics; Thermotoga maritima / genetics; Thermotoga maritima / metabolism; Transcription, Genetic
TL;DR:
The results reported here indicate that, while T. maritima shares core elements of the bacterial heat-shock response with mesophiles, the thermal stress regulatory strategies of this organism differ significantly, and it remains to be elucidated whether these differences are related to thermophilicity or phylogenetic placement.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
14. Life Below Water
(OpenAlex)
15. Life on Land
(Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018
2003 journal article
Carbohydrate-induced differential gene expression patterns in the hyperthermophilic bacterium Thermotoga maritima
JOURNAL OF BIOLOGICAL CHEMISTRY, 278(9), 7540–7552.
MeSH headings : Carbohydrate Metabolism; Carbohydrate Sequence; Carbohydrates / chemistry; Carbon / metabolism; DNA, Complementary / metabolism; Down-Regulation; Gene Expression Regulation, Bacterial; Hydrolases / chemistry; Hydrolases / metabolism; Molecular Sequence Data; Oligonucleotide Array Sequence Analysis; Open Reading Frames; Thermotoga maritima / metabolism; Up-Regulation
TL;DR:
The transcriptional response of T. maritima to specific carbohydrate growth substrates indicated that sugar backbone- and linkage-specific regulatory networks are operational in this organism during the uptake and utilization of carbohydrate substrates, and suggests that this organism is capable of adapting to a variety of growth environments containing carbohydrate growth substrate.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
7. Affordable and Clean Energy
(Web of Science)
12. Responsible Consumption and Production
(Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018
2003 journal article
Growth of hyperthermophilic Archaeon Pyrococcus futiosus on chitin involves two family 18 chitinases
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 69(6), 3119–3128.
MeSH headings : Amino Acid Sequence; Calorimetry; Chitin / metabolism; Chitinases / chemistry; Chitinases / classification; Chitinases / genetics; Chitinases / metabolism; Culture Media; Hexosaminidases / chemistry; Hexosaminidases / genetics; Hexosaminidases / metabolism; Hot Temperature; Molecular Sequence Data; Pyrococcus furiosus / enzymology; Pyrococcus furiosus / growth & development; Pyrococcus furiosus / metabolism; Recombinant Proteins / metabolism; Sequence Analysis, DNA
TL;DR:
Two open reading frames (chiA and chiB) were identified in the genome of P. furiosus, which encodes chitinases with sequence similarity to proteins from the glycosyl hydrolase family 18 in less-thermophilic organisms, indicating that these two enzymes work together to recruit chit in-based substrates for P.furiosus growth.
(via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018
2003 journal article
Heat shock response by the hyperthermophilic archaeon Pyrococcus furiosus
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 69(4), 2365–2371.
MeSH headings : Archaeal Proteins / genetics; Archaeal Proteins / metabolism; Blotting, Northern; Gene Expression Profiling; Gene Expression Regulation, Archaeal; Heat-Shock Response; Oligonucleotide Array Sequence Analysis; Protein Folding; Pyrococcus furiosus / genetics; Pyrococcus furiosus / metabolism; Transcription, Genetic
TL;DR:
Collective transcriptional analysis of heat shock response in the hyperthermophilic archaeon Pyrococcus furiosus was examined by using a targeted cDNA microarray in conjunction with Northern analyses and differential gene expression suggests that P.furiosus relies on a cooperative strategy of rescue, proteolysis, and stabilization to cope with polypeptide processing during thermal stress.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
14. Life Below Water
(OpenAlex)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018
2003 chapter
Hyperthermophiles
In Encyclopedia of Environmental Microbiology.
Sources: Crossref, NC State University Libraries
Added: August 7, 2021
2003 conference paper
Microbial ecology of hydrothermal biotypes
In R. B. Hoover & A. Y. Rozanov (Eds.), Proceedings: Optical science and technology, SPIE's 48th Annual Meeting (Vol. 5163).
Ed(s): R. Hoover & A. Rozanov
Event: Optical science and technology, SPIE's 48th Annual Meeting at San Diego, CA on August 3-8, 2003
author keywords: hyperthermophiles; extremophiles; biofilm; microbial ecology
TL;DR:
Study of the physiological and metabolic patterns in hyperthermophiles will shed light on microbial lifestyles consistent with putative hydrothermal niches on other planets and moons.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
14. Life Below Water
(OpenAlex)
15. Life on Land
(Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: September 11, 2021
2003 journal article
Strategic selection of hyperthermophilic esterases for resolution of 2-arylpropionic esters
BIOTECHNOLOGY PROGRESS, 19(5), 1410–1416.
MeSH headings : Amino Acid Sequence; Candida / enzymology; Candida / genetics; Catalysis; Enzyme Stability; Esterases / biosynthesis; Esterases / chemistry; Esterases / genetics; Esterases / isolation & purification; Esters; Hydrolysis; Molecular Sequence Data; Naproxen / analogs & derivatives; Naproxen / chemistry; Protein Engineering / methods; Pyrococcus furiosus / enzymology; Pyrococcus furiosus / genetics; Recombinant Proteins / biosynthesis; Recombinant Proteins / chemistry; Sequence Alignment / methods; Sequence Analysis, Protein / methods; Species Specificity; Stereoisomerism; Sulfolobus / enzymology; Sulfolobus / genetics
TL;DR:
The results here show that although bioinformatics tools can be used to select candidate biocatalysts for chiral resolution of 2‐arylpropionic esters, biochemical characterization is needed to definitively determine functional characteristics.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
6. Clean Water and Sanitation
(OpenAlex)
12. Responsible Consumption and Production
(Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018
2002 journal article
Biochemical characterization of Thermotoga maritima endoglucanase Ce174 with and without a carbohydrate binding module (CBM)
FEBS LETTERS, 531(2), 375–380.
MeSH headings : Amino Acid Sequence; Binding Sites; Carbohydrate Metabolism; Cellulase / chemistry; Cellulase / genetics; Cellulase / metabolism; Cellulose / metabolism; Chitinases / genetics; Molecular Sequence Data; Protein Structure, Tertiary; Pyrococcus furiosus / enzymology; Recombinant Fusion Proteins / metabolism; Sequence Alignment; Substrate Specificity; Thermotoga maritima / enzymology
TL;DR:
Based on the cleavage pattern determined for Tm Cel74 on glucan‐based substrates, this enzyme likely initiates recruitment of carbohydrate carbon and energy sources by creating oligosaccharides that are transported into the cell for further processing.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
7. Affordable and Clean Energy
(Web of Science)
12. Responsible Consumption and Production
(Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018
2002 patent
Compositions for fracturing subterranean formations
Washington, DC: U.S. Patent and Trademark Office.
Source: NC State University Libraries
Added: August 6, 2018
2002 journal article
Enantiomeric resolution of 2-aryl propionic esters with hyperthermophilic and mesophilic esterases: Contrasting thermodynamic mechanisms
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 124(28), 8190–8191.
MeSH headings : Candida / enzymology; Carboxylesterase; Carboxylic Ester Hydrolases / chemistry; Esterases / chemistry; Esters / chemistry; Esters / isolation & purification; Lipase / chemistry; Naproxen / chemistry; Naproxen / isolation & purification; Propionates / chemistry; Propionates / isolation & purification; Rhizomucor / enzymology; Stereoisomerism; Substrate Specificity; Sulfolobus / enzymology; Thermodynamics
TL;DR:
The enantiomeric resolution of 2-aryl propionic esters by hyperthermophilic and mesophilic esterases was found to be governed by contrasting thermodynamic mechanisms.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
12. Responsible Consumption and Production
(Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018
2002 chapter
Enzymatic Modification of Guar Solutions
In Z. Amjad (Ed.), Water Soluble Polymers (pp. 41–49).
Ed(s): Z. Amjad
Sources: Crossref, NC State University Libraries
Added: August 7, 2021
2002 journal article
Glucose-to-fructose conversion at high temperatures with xylose (glucose) isomerases from Streptomyces murinus and two hyperthermophilic Thermotoga species
BIOTECHNOLOGY AND BIOENGINEERING, 80(2), 185–194.
author keywords: Thermotoga; xylose isomerase; Streptomyces murinus; hyperthermophile
MeSH headings : Aldose-Ketose Isomerases / chemistry; Aldose-Ketose Isomerases / classification; Aldose-Ketose Isomerases / metabolism; Bioreactors; Enzyme Activation; Enzymes, Immobilized / metabolism; Fructose / biosynthesis; Glucose / metabolism; Gram-Negative Anaerobic Straight, Curved, and Helical Rods / classification; Gram-Negative Anaerobic Straight, Curved, and Helical Rods / enzymology; Gram-Negative Anaerobic Straight, Curved, and Helical Rods / metabolism; Hot Temperature; Hydrogen-Ion Concentration; Sensitivity and Specificity; Species Specificity; Streptomyces / classification; Streptomyces / enzymology; Thermotoga maritima / classification; Thermotoga maritima / enzymology
TL;DR:
In the presence of both cations, these enzymes showed the potential to catalyze glucose-to-fructose conversion at 80 degrees C with estimated lifetime productivities on the order of 2000 kg fructose per kilogram enzyme, a value competitive with enzymes currently used at 55 degrees to 65 degrees C, but with the additional advantage of higher fructose concentrations.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
12. Responsible Consumption and Production
(Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018
2002 journal article
Proteolysis in hyperthermophilic microorganisms
Archaea, 1(1), 63–74.
MeSH headings : Archaeal Proteins / genetics; Archaeal Proteins / metabolism; Bacterial Proteins / genetics; Bacterial Proteins / metabolism; Genome, Archaeal; Genome, Bacterial; Hot Temperature; Peptide Hydrolases / genetics; Peptide Hydrolases / metabolism; Pyrococcus / metabolism; Sulfolobus solfataricus / metabolism; Thermotoga maritima / genetics; Thermotoga maritima / metabolism
TL;DR:
Genome sequence data is exploited to exploit genome sequence data to compare hyperthermophilic microorganisms from the euryarchaeotal genus Pyrococcus, the crenarchaeote Sulfolobus solfataricus, and the bacterium Thermotoga maritima and revealed both similarities and differences in the mechanisms utilized for proteolysis by each of these hyperThermophiles.
(via Semantic Scholar)
Sources: Crossref, NC State University Libraries
Added: August 7, 2021
2002 journal article
Regulation of endo-acting glycosyl hydrolases in the hyperthermophilic bacterium Thermotoga maritima grown on glucan- and mannan-based polysaccharides
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 68(2), 545–554.
MeSH headings : Amino Acid Sequence; Culture Media; Gene Expression Regulation, Bacterial; Glucans / chemistry; Glucans / metabolism; Glycoside Hydrolases / chemistry; Glycoside Hydrolases / genetics; Glycoside Hydrolases / metabolism; Hot Temperature; Mannans / chemistry; Mannans / metabolism; Molecular Sequence Data; Polysaccharides / chemistry; Polysaccharides / metabolism; Recombinant Proteins / genetics; Recombinant Proteins / metabolism; Sequence Analysis, DNA; Thermotoga maritima / enzymology; Thermotoga maritima / genetics; Thermotoga maritima / growth & development
TL;DR:
The genome sequence of the hyperthermophilic bacterium Thermotoga maritima encodes a number of glycosyl hydrolases, and the intracellular localization of Cel5A suggest that the physiological role of this enzyme includes hydrolysis of glucomannan oligosaccharides that are transported following initial hydrolyzing by extracellular glycosidases, such as Man5.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
7. Affordable and Clean Energy
(Web of Science)
12. Responsible Consumption and Production
(Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018
2002 report
Saccharification enzymes from hyperthermophilic bacteria and processes for their production
(U.S. Patent No. 6,355,467).
Source: NC State University Libraries
Added: September 11, 2021
2002 journal article
Structural and catalytic response to temperature and cosolvents of carboxylesterase EST1 from the extremely thermoacidophilic archaeon Sulfolobus solfataricus P1
BIOTECHNOLOGY AND BIOENGINEERING, 80(7), 784–793.
author keywords: DMSO; conformational flexibility; enzyme activation; thermostable
MeSH headings : Acetonitriles / chemistry; Carboxylesterase; Carboxylic Ester Hydrolases / biosynthesis; Carboxylic Ester Hydrolases / chemistry; Carboxylic Ester Hydrolases / isolation & purification; Catalysis; Dimethyl Sulfoxide / chemistry; Dimethylformamide / chemistry; Dioxanes / chemistry; Enzyme Activation; Enzyme Stability; Molecular Conformation; Quality Control; Sensitivity and Specificity; Solvents / chemistry; Species Specificity; Structure-Activity Relationship; Sulfolobus / classification; Sulfolobus / enzymology; Temperature
TL;DR:
Results suggest cosolvents may also serve as activators in applications involving thermostable biocatalysts at sub-optimal temperatures, related to small changes in the enzyme's structure resulting in an increase in its conformational flexibility.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
6. Clean Water and Sanitation
(OpenAlex)
12. Responsible Consumption and Production
(Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018
2001 journal article
Bivalent cations and amino-acid composition contribute to the thermostability of Bacillus licheniformis xylose isomerase
EUROPEAN JOURNAL OF BIOCHEMISTRY, 268(23), 6291–6301.
author keywords: Bacillus licheniformis; metal binding; thermostability; xylose isomerase
MeSH headings : Aldose-Ketose Isomerases / chemistry; Aldose-Ketose Isomerases / classification; Aldose-Ketose Isomerases / genetics; Aldose-Ketose Isomerases / metabolism; Amino Acids / analysis; Bacillus / enzymology; Bacillus / genetics; Binding Sites; Cations, Divalent / pharmacology; Cloning, Molecular; Enzyme Stability / drug effects; Genes, Bacterial; Hydrogen-Ion Concentration; Kinetics; Recombinant Proteins / chemistry; Recombinant Proteins / genetics; Recombinant Proteins / metabolism; Substrate Specificity; Temperature; Thermodynamics
TL;DR:
Results suggest that the first irreversible event in BLXI unfolding is the release of one or both of its metals from the active site, suggesting that protein thermostability is a function of more complex molecular determinants than amino-acid content alone.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
12. Responsible Consumption and Production
(Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018
2001 chapter
Carboxylesterase from Sulfolobus solfataricus P1
In Methods in Enzymology (pp. 461–471).
MeSH headings : Amino Acid Sequence; Calorimetry, Differential Scanning; Carboxylesterase; Carboxylic Ester Hydrolases / chemistry; Carboxylic Ester Hydrolases / isolation & purification; Carboxylic Ester Hydrolases / metabolism; Electrophoresis, Polyacrylamide Gel; Molecular Sequence Data; Recombinant Proteins / chemistry; Recombinant Proteins / isolation & purification; Recombinant Proteins / metabolism; Sulfolobus / enzymology
TL;DR:
This chapter describes the cloning, expression, purification, and characterization of a recombinant carboxylesterase (Sso P1 carboxesterase) from the extreme thermoacidophile Sulfolobus solfataricus P1.
(via Semantic Scholar)
Source: Crossref
Added: July 21, 2021
2001 journal article
Carboxylesterase from Sulfolobus solfataricus P1
Hyperthermophilic Enzymes. Part A, 330, 461–471.
Source: NC State University Libraries
Added: August 6, 2018
2001 patent
Compositions for fracturing subterranean formations
Washington, DC: U.S. Patent and Trademark Office.
Source: NC State University Libraries
Added: August 6, 2018
2001 journal article
Continuous cultivation of hyperthermophiles
Methods in Enzymology, 330, 31–40.
MeSH headings : Archaea / growth & development; Archaea / metabolism; Bacteria / growth & development; Bacteria / metabolism; Bioreactors; Culture Media; Sulfur / metabolism
TL;DR:
This chapter describes a continuous culture system that can generate biomass from hyperthermophiles on a scale suitable for enzyme purification and has also been useful for studying the bioenergetics and physiology of hypertherms and for developing media formulations that induce enzyme expression.
(via Semantic Scholar)
Sources: NC State University Libraries, NC State University Libraries
Added: August 6, 2018
2001 journal article
Galactomannanases man2 and man5 from Thermotoga species: Growth physiology on galactomannans, gene sequence analysis, and biochemical properties of recombinant enzymes
BIOTECHNOLOGY AND BIOENGINEERING, 75(3), 322–333.
author keywords: Thermotoga; hyperthermophile; hemicellulase; glycosyl hydrolase; galactomannan; beta-mannanase; beta-mannosidase
MeSH headings : Amino Acid Sequence; Bacteria / enzymology; Bacteria / genetics; Bacteria / growth & development; Bacterial Proteins; Galactose / analogs & derivatives; Mannans / metabolism; Mannosidases / chemistry; Mannosidases / genetics; Mannosidases / metabolism; Microscopy, Electron; Molecular Sequence Data; Sequence Homology, Amino Acid; beta-Mannosidase
TL;DR:
The identification of the galactomannan-degrading enzymes in these Thermotoga species adds to the list of biotechnologically important hemicellulases produced by members of this hyperthermophilic genera.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
7. Affordable and Clean Energy
(Web of Science)
12. Responsible Consumption and Production
(Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018
2001 chapter
Homomultimeric protease and putative bacteriocin homolog from Thermotoga maritima
In Methods in Enzymology (pp. 455–460).
MeSH headings : Amino Acid Sequence; Biopolymers; Chromatography, Liquid / methods; Endopeptidases / chemistry; Endopeptidases / isolation & purification; Endopeptidases / metabolism; Microscopy, Electron; Molecular Sequence Data; Sequence Homology, Amino Acid; Thermotoga maritima / enzymology
TL;DR:
Although T. maritima appears to be motile, migrating at a speed proportional to the temperature, the reduction of sulfur does not provide an energetic boost as seen by the lack of effect on growth yields and fermentation balances.
(via Semantic Scholar)
Source: Crossref
Added: August 7, 2021
2001 journal article
Homomultimeric protease and putative bacteriocin homolog from Thermotoga maritima
Hyperthermophilic Enzymes. Part A, 330, 455–460.
Source: NC State University Libraries
Added: August 6, 2018
2001 report
Methods and compositions for fracturing subterranean formations
(U.S. Patent No. 6,197,730).
Source: NC State University Libraries
Added: September 11, 2021
2001 chapter
Protease I from Pyrococcus furiosus
In Methods in Enzymology (pp. 403–413).
MeSH headings : Archaeal Proteins; Chromatography, Liquid / methods; Cloning, Molecular; Electrophoresis, Polyacrylamide Gel; Molecular Weight; Peptide Hydrolases / genetics; Peptide Hydrolases / isolation & purification; Peptide Hydrolases / metabolism; Pyrococcus furiosus / enzymology; Recombinant Fusion Proteins / genetics; Recombinant Fusion Proteins / isolation & purification; Recombinant Fusion Proteins / metabolism
TL;DR:
This chapter describes several methods that have been used to purify PfpI directly from P. furiosus cell extracts, together with an assay to detect proteolytic activity, a procedure to determine its molecular mass, and approaches to minimize PfPI-catalyzed proteolysis of other P. furious proteins.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
15. Life on Land
(OpenAlex)
Source: Crossref
Added: August 7, 2021
2001 journal article
Protease I from Pyrococcus furiosus
Hyperthermophilic Enzymes. Part A, 330, 403–413.
Source: NC State University Libraries
Added: August 6, 2018
2001 chapter
Xylose isomerases from Thermotoga
In Methods in Enzymology (pp. 215–224).
MeSH headings : Aldose-Ketose Isomerases / chemistry; Aldose-Ketose Isomerases / genetics; Aldose-Ketose Isomerases / isolation & purification; Aldose-Ketose Isomerases / metabolism; Amino Acid Sequence; Calorimetry; Cloning, Molecular; Enzyme Stability; Escherichia coli / genetics; Gram-Negative Anaerobic Straight, Curved, and Helical Rods / enzymology; Gram-Negative Anaerobic Straight, Curved, and Helical Rods / growth & development; Hydrogen-Ion Concentration; Kinetics; Molecular Sequence Data
TL;DR:
XIs from the hyperthermophilic eubacteria Thermotoga maritima andThermotoga neapolitana are the most thermostable yet characterized XIs.
(via Semantic Scholar)
Source: Crossref
Added: August 7, 2021
2001 journal article
Xylose isomerases from Thermotoga
Hyperthermophilic Enzymes. Part A, 330, 215–224.
Source: NC State University Libraries
Added: August 6, 2018
2001 journal article
alpha-D-galactosidases from Thermotoga species
Hyperthermophilic Enzymes. Part A, 330, 246–260.
Source: NC State University Libraries
Added: August 6, 2018
2001 journal article
alpha-Glucosidase from Pyrococcus furiosus
Hyperthermophilic Enzymes. Part A, 330, 260–269.
Source: NC State University Libraries
Added: August 6, 2018
2001 journal article
beta-Endoglucanase from Pyrococcus furiosus
Hyperthermophilic Enzymes. Part A, 330, 346–354.
Source: NC State University Libraries
Added: August 6, 2018
2001 journal article
beta-Mannanases from Thermotoga species
Hyperthermophilic Enzymes. Part A, 330, 224–238.
Source: NC State University Libraries
Added: August 6, 2018
2001 journal article
beta-Mannosidase from Thermotoga species
Hyperthermophilic Enzymes. Part A, 330, 238–246.
Source: NC State University Libraries
Added: August 6, 2018
2001 chapter
α-Glucosidase from Pyrococcus furiosus
In Methods in Enzymology (pp. 260–269).
MeSH headings : Chromatography, Liquid / methods; Enzyme Stability; Escherichia coli / genetics; Hydrogen-Ion Concentration; Molecular Weight; Pyrococcus furiosus / enzymology; Recombinant Proteins / isolation & purification; Recombinant Proteins / metabolism; Temperature; alpha-Glucosidases / isolation & purification; alpha-Glucosidases / metabolism
TL;DR:
Despite the potential impact of hyperthermophilic enzymes on industrial processes, including starch conversion, their application is still largely unrealized, one readily apparent obstacle is developing a costefficient method for producing sufficient quantities of enzyme either directly from the source organism or through recombinant means.
(via Semantic Scholar)
Source: Crossref
Added: July 21, 2021
2001 chapter
αa-D-Galactosidases from Thermotoga species
In Methods in Enzymology (pp. 246–260).
MeSH headings : Base Sequence; Cloning, Molecular; DNA, Bacterial; Gram-Negative Anaerobic Straight, Curved, and Helical Rods / isolation & purification; Gram-Negative Anaerobic Straight, Curved, and Helical Rods / metabolism; Hydrogen-Ion Concentration; Recombinant Proteins / genetics; Recombinant Proteins / isolation & purification; Recombinant Proteins / metabolism; Temperature; alpha-Galactosidase / genetics; alpha-Galactosidase / isolation & purification; alpha-Galactosidase / metabolism
TL;DR:
This chapter discusses the purification, cloning, and expression of recombinant α Gal from T. neapolitana and T. maritima, which exhibit different biochemical properties in terms of pH optima, activity, and thermostability.
(via Semantic Scholar)
Source: Crossref
Added: August 7, 2021
2001 chapter
β-Endoglucanase from Pyrococcus furiosus
In Methods in Enzymology (pp. 346–354).
MeSH headings : Amino Acid Sequence; Base Sequence; Cellulase / biosynthesis; Cellulase / chemistry; Cellulase / isolation & purification; Cellulase / metabolism; DNA Primers; Hydrolysis; Molecular Sequence Data; Polysaccharides / chemistry; Polysaccharides / metabolism; Pyrococcus furiosus / enzymology; Recombinant Proteins / biosynthesis; Recombinant Proteins / chemistry; Recombinant Proteins / isolation & purification; Recombinant Proteins / metabolism; Substrate Specificity
TL;DR:
This chapter describes the approaches used for the cloning and expression in Escherichia coli of the eglA gene, which encodes the P. furiosus endoglucanase, and protocols used to study the biochemical properties of the recombinant enzyme.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
14. Life Below Water
(OpenAlex)
Source: Crossref
Added: August 7, 2021
2001 journal article
β-Mannanases from Thermotoga species
Methods in Enzymology, 330, 224–238.
MeSH headings : Amino Acid Sequence; Carbohydrate Conformation; Electrophoresis, Polyacrylamide Gel; Enzyme Stability; Galactose / analogs & derivatives; Gram-Negative Anaerobic Straight, Curved, and Helical Rods / enzymology; Mannans / chemistry; Mannosidases / chemistry; Mannosidases / isolation & purification; Mannosidases / metabolism; Molecular Sequence Data; Sequence Homology, Amino Acid; beta-Mannosidase
TL;DR:
This chapter describes the purification and characterization of a β -mannanase from T. neapolitana, as well as cloning and sequencing of this enzyme from T., which are useful in several industrial applications where thermostability and thermoactivity are desirable.
(via Semantic Scholar)
Source: NC State University Libraries
Added: September 11, 2021
2001 chapter
β-Mannosidase from Thermotoga species
In Methods in Enzymology (pp. 238–246).
MeSH headings : Chromatography, Gel; Chromatography, Ion Exchange; Cloning, Molecular; Gram-Negative Anaerobic Straight, Curved, and Helical Rods / enzymology; Mannosidases / genetics; Mannosidases / isolation & purification; Mannosidases / metabolism; beta-Mannosidase
TL;DR:
This chapter presents the protocols used to purify the β -mannosidase from T. Neapolitana together with the results of cloning and expression of genes encoding the analogous enzyme of T. maritima in Escherichia coli.
(via Semantic Scholar)
Source: Crossref
Added: July 21, 2021
2000 journal article
Characterization of extremely thermostable enzymatic breakers (α-1,6-galactosidase and β-1,4-mannanase) from the hyperthermophilic bacterium Thermotoga neapolitana 5068 for hydrolysis of guar gum
Biotechnology and Bioengineering, 52(2), 332–339.
author keywords: hyperthermophilic enzymes; enzyme breakers; hydraulic fracturing; hydrolysis galactomannan; guar gum
TL;DR:
It was determined that the purified beta-mannanase alone can substantially reduce viscosity of guar solutions, while the alpha-galactosidase alone had limited vis coscosity reduction activity.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
7. Affordable and Clean Energy
(Web of Science)
12. Responsible Consumption and Production
(Web of Science)
Source: Crossref
Added: August 7, 2021
2000 journal article
Effect of carbon and nitrogen sources on growth dynamics and exopolysaccharide production for the hyperthermophilic archaeon Thermococcus litoralis and bacterium Thermotoga maritima
BIOTECHNOLOGY AND BIOENGINEERING, 69(5), 537–547.
author keywords: hyperthermophile; archaea; bioenergetics; exopolysaccharide; wall growth
TL;DR:
The importance of effective cultivation methods for addressing physiological issues related to the growth of hyperthermophilic heterotrophs is illustrated, such that maltose utilization plots were biphasic and EPS production rose sharply at high dilution rates for both organisms.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
15. Life on Land
(Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018
1999 journal article
An endoglucanase, eglA, from the hyperthermophilic archaeon Pyrococcus furiosus hydrolyzes beta-1,4 bonds in mixed-linkage(1 -> 3),(1 -> 4)-beta-D-glucans and cellulose
Journal of Bacteriology, 181(1), 284–290.
Source: NC State University Libraries
Added: August 6, 2018
1999 article
Biocatalysis and biotransformation - Editorial overview
Kelly, R. M., & Waldmann, H. (1999, February). CURRENT OPINION IN CHEMICAL BIOLOGY, Vol. 3, pp. 9–10.
UN Sustainable Development Goal Categories
15. Life on Land
(Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018
1999 patent
Compositions for fracturing subterranean formations
Washington, DC: U.S. Patent and Trademark Office.
Source: NC State University Libraries
Added: August 6, 2018
1999 journal article
Continuous culture as a tool for investigating the growth physiology of heterotrophic hyperthermophiles and extreme thermoacidophiles
Journal of Applied Microbiology, 85(1999), 118S–127.
Source: NC State University Libraries
Added: August 6, 2018
1999 chapter
Cultivation of hyperthermophilic and extremely thermoacidophilic microorganisms
In A. L. Demain, J. E. Davies, & R. M. Atlas (Eds.), Manual of Industrial Microbiology and Biotechnology (2nd ed.). Washington, D.C.: ASM Press.
Ed(s): A. Demain, J. Davies & R. Atlas
Source: NC State University Libraries
Added: September 18, 2021
1999 chapter
Enzymes, Extremely Thermostable
In M. C. Flickinger & S. W. Drew (Eds.), Encyclopedia of Bioprocess Technology: Fermentation, Biocatalysis, and Bioseparation (pp. 987–1004).
Ed(s): M. Flickinger & S. Drew
TL;DR:
The discovery of Extremely Thermostable Enzyme Discovery and Uses: Reinforcement of Thermostability and Replacements of Existing Industrial Enzymes are highlighted.
(via Semantic Scholar)
Sources: NC State University Libraries, NC State University Libraries
Added: September 18, 2021
1999 journal article
Influence of polymolecular events on inactivation behavior of xylose isomerase from Thermotoga neapolitana 5068
BIOTECHNOLOGY AND BIOENGINEERING, 62(5), 509–517.
author keywords: Thermotoga neapolitana; xylose isomerase; deamidation; immobilization; inactivation
TL;DR:
The inactivation behavior of the xylose isomerase from Thermotoga neapolitana (TN5068 XI) was examined for both the soluble and immobilized enzyme, and formation of a reversible polymolecular aggregate capable of protecting the soluble enzyme from irreversible deactivation appears to be responsible for the second phase of inactivation seen.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
12. Responsible Consumption and Production
(Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018
1999 report
Methods and compositions for fracturing subterranean formations
(U.S. Patent No. 5,869,435).
Source: NC State University Libraries
Added: September 11, 2021
1999 journal article
Relationship between glycosyl hydrolase inventory and growth physiology of the hyperthermophile Pyrococcus furiosus on carbohydrate-based media
Applied and Environmental Microbiology, 65(3), 893–897.
Source: NC State University Libraries
Added: August 6, 2018
1999 journal article
Rheology and molecular weight changes during enzymatic degradation of a water-soluble polymer
MACROMOLECULES, 32(2), 294–300.
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
6. Clean Water and Sanitation
(OpenAlex)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018
1999 journal article
Synergistic interactions among beta-laminarinase, beta-1,4-glucanase, and beta-glucosidase from the hyperthermophilicarchaeon Pyrococcus furiosus during hydrolysis of beta-1,4-,beta-1,3-, and mixed-linked polysaccharides
Biotechnology and Bioengineering, 66(1), 51–60.
Sources: NC State University Libraries, NC State University Libraries
Added: August 6, 2018
1999 chapter
Thermophilic Microorganisms
In M. C. Flickinger & S. W. Drew (Eds.), Encyclopedia of Bioprocess Technology: Fermentation, Biocatalysis, and Bioseparation (pp. 2536–2552).
Ed(s): M. Flickinger & S. Drew
Sources: NC State University Libraries, NC State University Libraries
Added: September 18, 2021
1998 journal article
Biooxidation capacity of the extremely thermoacidophilic archaeon Metallosphaera sedula under bioenergetic challenge
BIOTECHNOLOGY AND BIOENGINEERING, 58(6), 617–624.
author keywords: thermoacidophile; chemolithotroph; heat shock; chemical stress; continuous culture
TL;DR:
It is suggested that biooxidation rates can be increased by strategic exposure of extreme thermoacidophiles to chemical or thermal stress, and this approach should be considered for improving process performance.
(via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018
1998 journal article
Finding and using hyperthermophilic enzymes
TRENDS IN BIOTECHNOLOGY, 16(8), 329–332.
MeSH headings : Archaeal Proteins / isolation & purification; Biotechnology / trends; Clinical Enzyme Tests / trends; Enzymes / isolation & purification; Hot Temperature
TL;DR:
The remaining task is the identification of opportunities to make strategic use of the thermoactivity and thermostability of hyperthermophilic enzymes.
(via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018
1998 review
Glycosyl hydrolases from hyperthermophilic microorganisms
[Review of ]. CURRENT OPINION IN BIOTECHNOLOGY, 9(2), 141–145.
MeSH headings : Archaea / physiology; Bacterial Physiological Phenomena; Biotechnology; Enzyme Stability; Glycoside Hydrolases / classification; Glycoside Hydrolases / physiology; Temperature
TL;DR:
Glycosyl hydrolases from hyperthermophiles are, thus far, the most widely studied enzyme class from these organisms and make them ideal candidates for the study of biocatalysis and protein thermostability at extremely high temperatures.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
7. Affordable and Clean Energy
(Web of Science)
12. Responsible Consumption and Production
(Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018
1998 journal article
Homomultimeric protease in the hyperthermophilic bacterium Thermotoga maritima has structural and amino acid sequence homology to bacteriocins in mesophilic bacteria
FEBS LETTERS, 440(3), 393–398.
author keywords: proteolysis; bacteriocin; Thermotoga
MeSH headings : Amino Acid Sequence; Bacteriocins / chemistry; Endopeptidases / chemistry; Endopeptidases / genetics; Endopeptidases / isolation & purification; Endopeptidases / metabolism; Microscopy, Electron; Molecular Sequence Data; Sequence Analysis; Sequence Homology, Amino Acid; Thermotoga maritima / enzymology; Thermotoga maritima / genetics; Thermotoga maritima / ultrastructure
TL;DR:
A novel homomultimeric protease (>669 kDa), based on 31 kDa subunits, was purified from cell extracts of the hyperthermophilic bacterium Thermotoga maritima, suggesting that their antibacterial activity may involve elements of proteolysis, and raises the prospect for anti‐microbial ecological strategies in hypertherMophilic niches.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
14. Life Below Water
(OpenAlex)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018
1998 journal article
Isolation and characterization of Thermococcus barossii, sp. nov., a hyperthermophilic Archaeon isolated from a hydrothermal vent flange formation
SYSTEMATIC AND APPLIED MICROBIOLOGY, 21(1), 40–49.
author keywords: Thermococcus barossii; hyperthermophiles; 16S rRNA; growth physiology
MeSH headings : Anti-Bacterial Agents / pharmacology; Chloramphenicol; Culture Media; DNA, Archaeal / analysis; DNA, Ribosomal / analysis; Microbial Sensitivity Tests; Pacific Ocean; Phylogeny; RNA, Ribosomal, 16S / genetics; Seawater; Sequence Analysis, DNA; Streptomycin; Thermococcus / classification; Thermococcus / drug effects; Thermococcus / growth & development; Thermococcus / isolation & purification; Thermococcus / ultrastructure
TL;DR:
Based on the high degree of similarity among members of the genus Termococcus for the criteria used thus far, aspects of enzymology may be an important mechanism of differenting one species from another.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
14. Life Below Water
(OpenAlex)
15. Life on Land
(Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018
1998 journal article
Purification and functional characterization of a chaperone from Methanococcus jannaschii
SYSTEMATIC AND APPLIED MICROBIOLOGY, 21(2), 173–178.
author keywords: thermophilic chaperonin; Methanococcus jannaschii
MeSH headings : Adenosine Triphosphatases / chemistry; Amino Acid Sequence; Animals; Archaeal Proteins / chemistry; Archaeal Proteins / isolation & purification; Archaeal Proteins / physiology; Blotting, Western; Chromatography, Gel; Cyanogen Bromide / chemistry; Electrophoresis, Polyacrylamide Gel; Gene Expression Regulation, Archaeal; Goats; Luciferases / chemistry; Luciferases / immunology; Luminescent Measurements; Methanococcus / chemistry; Molecular Chaperones / chemistry; Molecular Chaperones / isolation & purification; Molecular Chaperones / physiology; Molecular Sequence Data; Molecular Weight; Protein Folding; Rabbits; Sequence Alignment; Sequence Analysis; Sequence Homology, Amino Acid
TL;DR:
To the authors' knowledge, this is the first functional characterization of a chaperone from a methanogen and is the predicted protein product of the previously identified chaperonin gene in M. jannaschii.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018
1998 chapter
Pyrococcus furiosus Protease I (PfpI)
In F. Woessner, N. Rawlings, & A. Barrett (Eds.), Handbook of Proteolytic Enzymes. London: Academic Press Limited.
Ed(s): F. Woessner, N. Rawlings & A. Barrett
Source: NC State University Libraries
Added: September 18, 2021
1998 journal article
The family 1 beta-glucosidases from Pyrococcus furiosus and Agrobacterium faecalis share a common catalytic mechanism
BIOCHEMISTRY, 37(49), 17170–17178.
MeSH headings : 1-Deoxynojirimycin / pharmacology; Binding, Competitive; Calorimetry, Differential Scanning; Catalysis / drug effects; Gluconates / pharmacology; Glucose / pharmacology; Hot Temperature; Hydrogen-Ion Concentration; Lactones; Pyrococcus furiosus / enzymology; Recombinant Proteins / antagonists & inhibitors; Recombinant Proteins / chemistry; Recombinant Proteins / metabolism; Rhizobium / enzymology; Substrate Specificity / drug effects; Thioglucosides / pharmacology; beta-Glucosidase / antagonists & inhibitors; beta-Glucosidase / genetics; beta-Glucosidase / metabolism
TL;DR:
The recombinant beta-glucosidase from the hyperthermophilic archaeon Pyrococcus furiosus was recombinantly produced in Escherichia coli and shown to have biophyscial and biochemical properties identical to those of the wild-type enzyme.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
7. Affordable and Clean Energy
(Web of Science; OpenAlex)
12. Responsible Consumption and Production
(Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018
1998 journal article
Thermotoga neapolitana homotetrameric xylose isomerase is expressed as a catalytically active and thermostable dimer in Escherichia coli
Applied and Environmental Microbiology, 64(7), 2357–2360.
Source: NC State University Libraries
Added: August 6, 2018
1997 journal article
Acquired thermotolerance and stressed-phase growth of the extremely thermoacidophilic Archaeon Metallosphaera sedula in continuous culture
Applied and Environmental Microbiology, 63(6), 2391–2396.
Source: NC State University Libraries
Added: August 6, 2018
1997 journal article
Hydrogen transfer between methanogens and fermentative heterotrophs in hyperthermophilic cocultures
Biotechnology and Bioengineering, 56(3), 268–278.
Sources: NC State University Libraries, NC State University Libraries
Added: August 6, 2018
1997 journal article
Purification and Characterization of a Proteasome from the Hyperthermophilic Archaeon Pyrococcus furiosus
Applied and Environmental Microbiology, 63(3), 1160–1164.
TL;DR:
A 640-kDa proteasome consisting of (alpha) (25-k da) and (beta) (22-k Da) subunits, and with a temperature optimum of 95(deg)C, was purified from crude cell extracts of a hyperthermophilic archaeon, Pyrococcus furiosus.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Crossref, NC State University Libraries
Added: July 21, 2021
1997 journal article
Purification and characterization of extremely thermostable beta mannanase, beta mannosidase, and alpha galactosidase from the hyperthermophilic eubacterium Thermotoga neapolitana
Applied and Environmental Microbiology, 63(1), 169–177.
Source: NC State University Libraries
Added: August 6, 2018
1997 journal article
Purification and characterization of two functional forms of intracellular protease PFPI from the hyperthermophilic Archaeon Pyrococcus furiosus
Applied and Environmental Microbiology, 63(1), 289–295.
Source: NC State University Libraries
Added: August 6, 2018
1997 patent
Purified Thermococcus barossii DNA polymerase
Washington, DC: U.S. Patent and Trademark Office.
Source: NC State University Libraries
Added: August 6, 2018
1997 journal article
Viscosity Reduction Of Hydraulic Fracturing Fluids Through Enzymatic Hydrolysis
SPE Journal, 2(02), 204–212.
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: Crossref
Added: July 21, 2021
1996 journal article
Comparison of a β-Glucosidase and a β-Mannosidase from the Hyperthermophilic Archaeon Pyrococcus furiosus
Journal of Biological Chemistry, 271(39), 23749–23755.
MeSH headings : Amino Acid Sequence; Archaea / enzymology; Genes, Bacterial; Isoelectric Point; Kinetics; Mannosidases / isolation & purification; Mannosidases / metabolism; Molecular Sequence Data; Molecular Weight; Sequence Alignment; Sequence Homology, Amino Acid; Substrate Specificity; beta-Glucosidase / isolation & purification; beta-Glucosidase / metabolism; beta-Mannosidase
TL;DR:
Two distinct exo-acting, β-specific glycosyl hydrolases were purified to homogeneity from crude cell extracts of the hyperthermophilic archaeon Pyrococcus furiosus: a β-glucosidase, corresponding to the one previously purified by Kengen et al. (Kengen, S. W. M., Luesink, E. J., and Zehnder, A. J).
(via Semantic Scholar)
UN Sustainable Development Goal Categories
7. Affordable and Clean Energy
(Web of Science)
12. Responsible Consumption and Production
(Web of Science)
14. Life Below Water
(OpenAlex)
Sources: Crossref, NC State University Libraries
Added: July 21, 2021
1996 journal article
Confessions of a graduate student recruiter
Chemical Engineering Education, 30(4), 262–265.
Source: NC State University Libraries
Added: September 11, 2021
1996 journal article
Growth Physiology of the Hyperthermophilic Archaeon Thermococcus litoralis: Development of a Sulfur-Free Defined Medium, Characterization of an Exopolysaccharide, and Evidence of Biofilm Formation
Applied and Environmental Microbiology, 62(12), 4478–4485.
TL;DR:
Nutritional characteristics of the hyperthermophilic archaeon Thermococcus litoralis have been investigated with emphasis on the development of a sulfur-free, defined growth medium, analysis of an exocellular polysaccharide, and formation of a biofilm.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
14. Life Below Water
(OpenAlex)
15. Life on Land
(Web of Science)
Sources: Crossref, NC State University Libraries
Added: July 21, 2021
1996 chapter
Proteases and Glycosyl Hydrolases from Hyperthermophilic Microorganisms
In Advances in Protein Chemistry (pp. 271–310).
MeSH headings : Archaea / enzymology; Glycoside Hydrolases / chemistry; Glycoside Hydrolases / genetics; Glycoside Hydrolases / metabolism; Hot Temperature; Molecular Sequence Data; Peptide Hydrolases / chemistry; Peptide Hydrolases / genetics; Peptide Hydrolases / metabolism
TL;DR:
This chapter discusses hydrolytic enzymes proteases and glycosyl hydrolases, which provide clues to the evolution of physiological functions, metabolic pathways, and protein structure in hyperthermophiles involved in polypeptide and carbohydrate modifications.
(via Semantic Scholar)
Sources: Crossref, NC State University Libraries
Added: August 7, 2021
1996 journal article
Sequence, expression in Escherichia coli, and analysis of the gene encoding a novel intracellular protease (PfpI) from the hyperthermophilic archaeon Pyrococcus furiosus
Journal of Bacteriology, 178(9), 2605–2612.
MeSH headings : Amino Acid Sequence; Archaea / enzymology; Archaea / genetics; Archaeal Proteins; Bacterial Proteins / chemistry; Bacterial Proteins / genetics; Bacterial Proteins / isolation & purification; Bacterial Proteins / metabolism; Base Sequence; Cloning, Molecular; Enzyme Stability; Escherichia coli / genetics; Gene Expression; Genes, Bacterial / genetics; Hot Temperature; Molecular Sequence Data; Molecular Weight; Peptide Hydrolases / chemistry; Peptide Hydrolases / genetics; Peptide Hydrolases / isolation & purification; Peptide Hydrolases / metabolism; Promoter Regions, Genetic / genetics; Protein Conformation; Recombinant Fusion Proteins / biosynthesis; Recombinant Fusion Proteins / chemistry; Restriction Mapping; Sequence Analysis, DNA; Sequence Homology, Nucleic Acid
TL;DR:
A previously identified intracellular proteolytic activity in the hyperthermophilic archaeon Pyrococcus furiosus was found to be a homomultimer consisting of 18.8-kDa subunits; an immunological relationship of this protease to the eukaryotic proteasome has been seen previously.
(via Semantic Scholar)
Sources: Crossref, NC State University Libraries
Added: August 7, 2021
1995 chapter
Biochemical engineering
In S. P. Parker (Ed.), McGraw-Hill Encyclopedia of Science and Technology (8th ed.). New York: McGraw-Hill.
Ed(s): S. Parker
Source: NC State University Libraries
Added: September 18, 2021
1995 journal article
Bioenergetic Response of the Extreme Thermoacidophile Metallosphaera sedula to Thermal and Nutritional Stresses
Applied and Environmental Microbiology, 61(6), 2314–2321.
Sources: Crossref, NC State University Libraries
Added: August 7, 2021
1995 chapter
Continuous culture techniques for extremely thermophilic and hyperthermophilic microorganisms
In F. T. Robb (Ed.), Archaea - A Laboratory Manual. Cold Spring Harbor Press.
Ed(s): F. Robb
Source: NC State University Libraries
Added: September 18, 2021
1995 journal article
Enzymes From Microorganisms in Extreme Environments
Chemical & Engineering News Archive, 73(51), 32–42.
UN Sustainable Development Goal Categories
15. Life on Land
(Web of Science)
Sources: Crossref, NC State University Libraries
Added: August 7, 2021
1995 journal article
Extremozymes: Expanding the Limits of Biocatalysis
Nature Biotechnology, 13(7), 662–668.
MeSH headings : Catalysis; Ecosystem; Enzymes / metabolism; Hydrogen-Ion Concentration; Osmolar Concentration; Pressure; Solvents; Temperature
TL;DR:
Given the information acquired from the study of extremozymes, modification of enzymes to improve their ranges of stability and activity remains a possibility and by expanding the range of conditions suitable for enzyme function, new opportunities to use biocatalysis will be created.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
12. Responsible Consumption and Production
(Web of Science)
Sources: Crossref, NC State University Libraries
Added: July 21, 2021
1995 patent
Methods and compositions for fracturing subterranean formations
Washington, DC: U.S. Patent and Trademark Office.
Source: NC State University Libraries
Added: August 6, 2018
1995 report
Proteolytic enzymes from hyperthermophilic bacteria and processes for their production
(U.S. Patent No. 5,391,489).
Source: NC State University Libraries
Added: September 11, 2021
1995 journal article
xylA cloning and sequencing and biochemical characterization of xylose isomerase from Thermotoga neapolitana
Applied and Environmental Microbiology, 61(5), 1867–1875.
MeSH headings : Aldose-Ketose Isomerases; Amino Acid Sequence; Bacteria / enzymology; Bacterial Proteins / genetics; Bacterial Proteins / metabolism; Base Sequence; Carbohydrate Epimerases / classification; Carbohydrate Epimerases / genetics; Carbohydrate Epimerases / metabolism; Cloning, Molecular; DNA, Bacterial / genetics; Genes, Bacterial; Gram-Negative Anaerobic Bacteria / enzymology; Gram-Negative Anaerobic Bacteria / genetics; Hot Temperature; Hydrogen-Ion Concentration; Molecular Sequence Data; Protein Denaturation; Recombinant Fusion Proteins / metabolism; Sequence Homology, Amino Acid; Species Specificity; Substrate Specificity
TL;DR:
Comparisons with other xylose isomerases showed that numbers of asparagine and glutamine residues decreased with increasing enzyme thermostability, presumably as a thermophilic strategy for diminishing the potential for chemical denaturation through deamidation at elevated temperatures.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
12. Responsible Consumption and Production
(Web of Science)
Sources: Crossref, NC State University Libraries
Added: July 21, 2021
1994 journal article
Extremely Thermophilic Microorganisms: Metabolic strategies, genetic characteristics and biotechnological potential of extremely thermophilic microorganisms
Annals of the New York Academy of Sciences, 745(1), 409–425.
Sources: NC State University Libraries, NC State University Libraries
Added: September 18, 2021
1994 journal article
Life in boiling water
Chemistry in Britain, 30(7), 555–558.
Source: NC State University Libraries
Added: September 18, 2021
1994 review
METABOLISM IN HYPERTHERMOPHILIC MICROORGANISMS
[Review of ]. ANTONIE VAN LEEUWENHOEK INTERNATIONAL JOURNAL OF GENERAL AND MOLECULAR MICROBIOLOGY, 66(1-3), 247–270.
author keywords: ARCHAEA; HYPERTHERMOPHILES; PROTEOLYTIC; SACCHAROLYTIC; SULFUR
MeSH headings : Anaerobiosis; Archaea / metabolism; Bacteria, Anaerobic / metabolism; Carbohydrate Metabolism; Carbon Dioxide / metabolism; Electron Transport; Energy Metabolism; Hot Temperature; Oxidoreductases / metabolism; Peptides / metabolism; Sulfur / metabolism
TL;DR:
Two So-reducing enzymes termed sulfhydrogenase and sulfide dehydrogenase have been purified from the cytoplasm of a hyperthermophile that is able to grow either with or without So, and a scheme for electron flow during the oxidation of carbohydrates and peptides and the reduction of So has been proposed.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
15. Life on Land
(Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018
1994 journal article
Thermostability and thermoactivity of enzymes from hyperthermophilic archaea
Bioorganic & Medicinal Chemistry, 2(7), 659–667.
MeSH headings : Archaea / classification; Archaea / enzymology; Archaea / genetics; DNA-Directed DNA Polymerase / chemistry; DNA-Directed DNA Polymerase / isolation & purification; DNA-Directed DNA Polymerase / metabolism; Enzyme Stability; Hot Temperature; Hydrolases / chemistry; Hydrolases / isolation & purification; Hydrolases / metabolism; Kinetics; Oxidoreductases / chemistry; Oxidoreductases / isolation & purification; Oxidoreductases / metabolism; Phylogeny; Thermodynamics
TL;DR:
Enzymes from hyperthermophilic microorganisms are characteristically thermostable and thermoactive at extremely high temperatures and information about the basis for the structure and function of these novel proteins is beginning to emerge.
(via Semantic Scholar)
Sources: Crossref, NC State University Libraries
Added: August 7, 2021
1993 chapter
Biocatalysis at extreme temperatures
In G. Georgiou & I. Glowinski (Eds.), Research opportunities in biomolecular engineering : the interface between chemical engineering and biology, December 7-8, 1992, proceedings. Bethesda, MD: NIH Press.
Ed(s): G. Georgiou & I. Glowinski
Event: Research opportunities in biomolecular engineering on December 7-8, 1992
Source: NC State University Libraries
Added: September 18, 2021
1993 chapter
Biocatalysis: An overview
In G. Georgiou & I. Glowinski (Eds.), Research opportunities in biomolecular engineering : the interface between chemical engineering and biology, December 7-8, 1992, proceedings. Bethesda, MD: NIH Press.
Ed(s): G. Georgiou & I. Glowinski
Source: NC State University Libraries
Added: July 27, 2021
1993 journal article
Bioenergetics of sulfur reduction in the hyperthermophilic archaeon Pyrococcus furiosus
Journal of Bacteriology, 175(6), 1823–1830.
MeSH headings : Archaea / enzymology; Archaea / metabolism; Ecology; Energy Metabolism; Fermentation; Hydrogen / metabolism; Metalloendopeptidases; Oxidation-Reduction; Peptide Hydrolases / metabolism; Regression Analysis; Sulfur / chemistry; Sulfur / metabolism; alpha-Glucosidases / metabolism
TL;DR:
The results suggest either S0 reduction is an energy-conserving reaction, i.e., S0 respiration, or that S0 has a stimulatory effect on or helps overcome a process that is yield limiting.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
7. Affordable and Clean Energy
(Web of Science)
Sources: Crossref, NC State University Libraries
Added: July 21, 2021
1993 journal article
Bioenergetics of the metal/sulfur-oxidizing extreme thermoacidophile, Metallosphaera sedula
Fuel, 72(12), 1619–1624.
author keywords: MICROBIAL DESULFURIZATION; COAL; PYRITE
TL;DR:
The relation between the organism's metabolic energy sources (sulfur, iron pyrite, organic compounds) and intracellular energy-requiring reactions is being studied and it is hoped that this framework can be used to evaluate and improve the various microbial options for inorganic sulfur removal from coal.
(via Semantic Scholar)
Sources: Crossref, NC State University Libraries
Added: July 21, 2021
1993 journal article
Characterization of Amylolytic Enzymes, Having Both α-1,4 and α-1,6 Hydrolytic Activity, from the Thermophilic Archaea Pyrococcus furiosus and Thermococcus litoralis
Applied and Environmental Microbiology, 59(8), 2614–2621.
TL;DR:
The identification of these enzymes further defines the saccharide-metabolizing systems possessed by these two organisms and appears to represent highly thermostable amylopullulanases.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
14. Life Below Water
(OpenAlex)
Sources: Crossref, NC State University Libraries
Added: August 7, 2021
1993 journal article
Enzymes from high-temperature microorganisms
Current Opinion in Biotechnology, 4(2), 188–192.
MeSH headings : Archaea / enzymology; Enzyme Stability; Enzymes / metabolism; Temperature
TL;DR:
Enzymes derived from microorganisms growing at extreme temperatures are of biotechnological use as highly thermostable biocatalysts and should provide insight into the intrinsic basis of protein stability.
(via Semantic Scholar)
Sources: Crossref, NC State University Libraries
Added: July 21, 2021
1993 journal article
Hydrogenase of the hyperthermophile Pyrococcus furiosus is an elemental sulfur reductase or sulfhydrogenase: evidence for a sulfur-reducing hydrogenase ancestor.
Proceedings of the National Academy of Sciences, 90(11), 5341–5344.
author keywords: HYDROGEN ACTIVATION; POLYSULFIDE REDUCTION; GEOTHERMAL BIOLOGY; EVOLUTION
MeSH headings : Archaea / enzymology; Biological Evolution; Chromatography; Chromatography, Ion Exchange; Chromatography, Liquid; Durapatite; Hot Temperature; Hydrogen-Ion Concentration; Hydrogenase / isolation & purification; Hydrogenase / metabolism; Hydroxyapatites; Kinetics; Oxidoreductases / isolation & purification; Oxidoreductases / metabolism; Species Specificity
TL;DR:
P purified hydrogenases from both hyperthermophilic and mesophilic representatives of the archaeal and bacterial domains were shown to reduce S0 to H2S, suggesting that the function of some form of ancestral hydrogenase was S0 reduction rather than, or in addition to, the reduction of protons.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
7. Affordable and Clean Energy
(Web of Science)
14. Life Below Water
(OpenAlex)
Sources: Crossref, NC State University Libraries
Added: July 21, 2021
1993 journal article
Influence of tungsten on metabolic patterns in Pyrococcus furiosus, a hyperthermophilic archaeon
Archives of Microbiology, 159(4), 380–385.
author keywords: PYROCOCCUS-FURIOSUS; HYPERTHERMOPHILIC ARCHAEA; MALTOSE FERMENTATION; PEPTIDE FERMENTATION; TUNGSTEN; ALPHA-GLUCOSIDASE; ALDEHYDE FERREDOXIN OXIDOREDUCTASE
TL;DR:
The addition of tungsten to cells growing in maltose/peptide medium dramatically reduced the specific activity of intracellular proteases, suggesting a preference for the utilization of maltose over peptides as the carbon and energy source in the presence of tngsten.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
14. Life Below Water
(OpenAlex)
Sources: Crossref, NC State University Libraries
Added: August 7, 2021
1993 report
Method for glucose isomerization using xylose isomerase purified from Thermotoga maritima and Thermotoga neapolitana
(US Patent No. 5,268,280).
Source: NC State University Libraries
Added: September 18, 2021
1993 report
Novel Isomerization Enzymes
(U.S. Patent No. 5,219,751).
Source: NC State University Libraries
Added: September 18, 2021
1993 report
Proteolytic Enzymes from Hyperthermophilic Bacteria and Processes for their Production
(US Patent No. 5,242,817).
Source: NC State University Libraries
Added: September 18, 2021
1993 journal article
Purification and characterization of a highly thermostable glucose isomerase produced by the extremely thermophilic eubacterium,Thermotoga maritima
Biotechnology and Bioengineering, 41(9), 878–886.
author keywords: THERMOTOGA - MARITIMA; THERMOPHILE; GLUCOSE ISOMERASE
TL;DR:
The high degree of thermostability, coupled with a neutral to slightly acid pH optimum, reveal this enzyme to be a promising candidate for improvement of the industrial glucose isomerization process.
(via Semantic Scholar)
Sources: Crossref, NC State University Libraries
Added: August 7, 2021
1993 journal article
Purification and characterization of a novel amylolytic enzyme from ES 4, a marine hyperthermophilic archaeum
Molecular Marine Biology and Biotechnology, 2(2), 76–87.
Source: NC State University Libraries
Added: September 18, 2021
1993 journal article
Regulation of ribosomal RNA transcription by growth rate of the hyperthermophilic Archaeon,Pyrococcus furiosus
FEMS Microbiology Letters, 111(2-3), 159–164.
MeSH headings : Archaea / genetics; Archaea / growth & development; Base Sequence; Genes, Bacterial; Hot Temperature; Molecular Sequence Data; Oligodeoxyribonucleotides / genetics; RNA, Bacterial / genetics; RNA, Ribosomal / genetics; RNA, Ribosomal, 16S / genetics; RNA, Ribosomal, 23S / genetics; Transcription, Genetic
TL;DR:
The single rRNA gene cluster from the Archaeon, Pyrococcus furiosus, grows optimally at 100 degrees C and is thus a hyperthermophile and transcription of 16S rRNA is subject to regulation over a 7.5-fold range in response to a 20-fold increase in growth rate.
(via Semantic Scholar)
Sources: Crossref, NC State University Libraries
Added: August 7, 2021
1992 journal article
TL;DR:
Biocatalysis Near and Above 100 C: An Overview Metabolic Enzymes from Sulfur-Dependent, Extremely Thermophilic Organisms Characterization of Enz enzymes from High-Temperature Bacteria Thermally Stable Urease from Thermophile Bacteria Respiratory Electron-Transport Components in Hyperthermophobic Bacteria.
(via Semantic Scholar)
Sources: Crossref, NC State University Libraries
Added: August 7, 2021
1992 chapter
Characterization of Enzymes from High-Temperature Bacteria
In ACS Symposium Series (pp. 23–41).
Sources: Crossref, NC State University Libraries
Added: July 21, 2021
1992 chapter
Metabolic Enzymes from Sulfur-Dependent, Extremely Thermophilic Organisms
In ACS Symposium Series: Vol. 498. Biocatalysis at Extreme Temperatures (pp. 4–22).
Sources: Crossref, NC State University Libraries
Added: August 7, 2021
1992 journal article
Physiological and Biochemical Characteristics of Pyrococcus furiosus, a Hyperthermophilic Archaebacterium
Annals of the New York Academy of Sciences, 665(1 Biochemical E), 309–319.
Sources: Crossref, NC State University Libraries
Added: July 21, 2021
1992 journal article
Regulation of Proteolytic Activity in the Hyperthermophile Pyrococcus furiosus
Applied and Environmental Microbiology, 58(4), 1134–1141.
TL;DR:
Under peptide-limiting conditions, levels of the 66-kDa protease (S66) were enhanced relative to those of other cell proteins, and preliminary evidence suggests that this protease is immunologically related to the eukaryotic multicatalytic proteinase complex (proteosome).
(via Semantic Scholar)
UN Sustainable Development Goal Categories
7. Affordable and Clean Energy
(OpenAlex)
Sources: Crossref, NC State University Libraries
Added: August 7, 2021
1991 journal article
Chemical and microbiological problems associated with research on the biodesulfurization of coal. A review
Resources, Conservation and Recycling, 5(2-3), 183–193.
Sources: Crossref, NC State University Libraries
Added: August 7, 2021
1991 journal article
Coal sulphur transformations monitored by hyperthermophilic archaebacteria
Fuel, 70(5), 599–604.
author keywords: MICROBIAL ACTIVITY; COAL; SULFUR
Sources: Crossref, NC State University Libraries
Added: August 7, 2021
1991 conference paper
Mixed Cultures of High Temperature Bacteria: Prospects for Bioprocessing of Fossil Fuels
Proceedings of the 2nd International Conference on the Biological Processing of Fossil Fuels. Presented at the 2nd International Conference on the Biological Processing of Fossil Fuels.
Event: 2nd International Conference on the Biological Processing of Fossil Fuels
Source: NC State University Libraries
Added: September 18, 2021
1990 journal article
Characterization of Amylolytic Enzyme Activities Associated with the Hyperthermophilic Archaebacterium Pyrococcus furiosus
Applied and Environmental Microbiology, 56(7), 1985–1991.
TL;DR:
The hyperthermophilic archaebacterium Pyrococcus furiosus produces several amylolytic enzymes in response to the presence of complex carbohydrates in the growth medium, suggesting that polysaccharide availability in its growth environment is a significant aspect of the niche from which it was isolated.
(via Semantic Scholar)
Sources: Crossref, NC State University Libraries
Added: August 7, 2021
1990 journal article
Characterization of sodium dodecyl sulfate-resistant proteolytic activity in the hyperthermophilic archaebacterium Pyrococcus furiosus
Applied and Environmental Microbiology, 56(7), 1992–1998.
MeSH headings : Archaea / enzymology; Bacteria / enzymology; Hot Temperature; Immunoblotting; Molecular Weight; Peptide Hydrolases / isolation & purification; Peptide Hydrolases / metabolism; Protease Inhibitors / pharmacology; Protein Denaturation / drug effects; Sodium Dodecyl Sulfate / pharmacology
TL;DR:
Cell extracts from Pyrococcus furiosus were found to contain five proteases, one of which (S66) are resistant to sodium dodecyl sulfate (SDS) denaturation, and results indicate that S66 is a serine protease.
(via Semantic Scholar)
Sources: Crossref, NC State University Libraries
Added: August 7, 2021
1990 journal article
EFFECT OF HETEROCYCLIC AMINE ADDITIVES ON THE ABSORPTION RATES OF CARBONYL SULFIDE AND CARBON DIOXIDE IN AQUEOUS METHYLDIETHANOLAMINE SOLUTIONS
Chemical Engineering Communications, 93(1), 181–191.
UN Sustainable Development Goal Categories
6. Clean Water and Sanitation
(OpenAlex)
Sources: Crossref, NC State University Libraries
Added: July 21, 2021
1990 journal article
Hydrogen-Sulfur Autotrophy in the Hyperthermophilic Archaebacterium,Pyrodictium brockii
Biotechnology and Genetic Engineering Reviews, 8(1), 345–378.
MeSH headings : Aerobiosis; Anaerobiosis; Archaea / growth & development; Archaea / metabolism; Hot Temperature; Hydrogen / metabolism; Oxidation-Reduction; Sulfur / metabolism
TL;DR:
This work focused on the elucidation of physiological aspects of the hyperthermophilic archaea Pyrodictium brockii and Pyrococcus furiosus and demonstrated the bioenergetic importance of a novel glycolytic pathway centered around a previously reported tungsten- requiring oxido-reductase.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
6. Clean Water and Sanitation
(OpenAlex)
Sources: Crossref, NC State University Libraries
Added: August 7, 2021
1990 chapter
Microbiological Processing of Coal
In D. L. Wise (Ed.), Biotechnology Applied to Fossil Fuels. New York: Marcel Dekker.
Ed(s): D. Wise
Source: NC State University Libraries
Added: September 18, 2021
1990 journal article
Microorganisms at extreme temperatures and pressures: Engineering insights
CHEMTECH, 20(11), 641–648.
Source: NC State University Libraries
Added: September 18, 2021
1990 journal article
Purification and characterization of an alpha-glucosidase from a hyperthermophilic archaebacterium, Pyrococcus furiosus, exhibiting a temperature optimum of 105 to 115 degrees C
Journal of Bacteriology, 172(7), 3654–3660.
MeSH headings : Archaea / enzymology; Archaea / growth & development; Bacteria / enzymology; Carbohydrate Metabolism; Chromatography; Chromatography, DEAE-Cellulose; Chromatography, Gel; Durapatite; Enzyme Stability; Hot Temperature; Hydroxyapatites; Kinetics; Molecular Weight; Substrate Specificity; Thermodynamics; alpha-Glucosidases / isolation & purification; alpha-Glucosidases / metabolism
TL;DR:
Purified alpha-glucosidase from P. furiosus exhibited remarkable thermostability; incubation of the enzyme at 98 degrees C resulted in a half life of nearly 48 h, and the enzyme exhibited optimum activity at pH 5.0 to 6.0 and over a temperature range of 105 to 115 degrees C.
(via Semantic Scholar)
Sources: Crossref, NC State University Libraries
Added: August 7, 2021
1990 journal article
Role of Polysulfides in Reduction of Elemental Sulfur by the Hyperthermophilic Archaebacterium Pyrococcus furiosus
Applied and Environmental Microbiology, 56(5), 1255–1262.
TL;DR:
Polysulfides formed through the breakdown of elemental sulfur or other sulfur compounds were found to be reduced to H(2)S by the hyperthermophilic archaebacterium Pyrococcus furiosus during growth.
(via Semantic Scholar)
Sources: Crossref, NC State University Libraries
Added: August 7, 2021
1990 report
Specific Biocatalysis for Coal Sulfur Speciation and Removal
(Electric Power Research Institute Interim Report No. ER/GS-6624).
Source: NC State University Libraries
Added: September 18, 2021
1990 journal article
The Hyperthermophilic Archaebacterium, Pyrococcus furiosus.
Annals of the New York Academy of Sciences, 589(1 Biochemical E), 301–314.
MeSH headings : Archaea / growth & development; Archaea / metabolism; Bacteria / growth & development; Bacteriological Techniques; Culture Media; Sulfur / metabolism
TL;DR:
From this brief discussion, it is clear that there are many obstacles to overcome before hyperthermophilic archaebacteria will be an important aspect of biotechnology.
(via Semantic Scholar)
Sources: Crossref, NC State University Libraries
Added: July 21, 2021
1989 conference paper
Bioprocessing of Fossil Fuels Using Hyperthermophilic Archaebacteria
Proceedings of the 14th Annual EPRI Conference on Fuel Science and Conversion. Presented at the 14th Annual EPRI Conference on Fuel Science and Conversion, Palo Alto, CA.
Event: 14th Annual EPRI Conference on Fuel Science and Conversion at Palo Alto, CA
Source: NC State University Libraries
Added: September 18, 2021
1989 journal article
Bioreactor operation for the production of exotoxin A byPseudomonas aeruginosa
Biotechnology and Bioengineering, 34(9), 1214–1220.
TL;DR:
Using established techniques from molecular biology, information on ETA mRNA levels could be obtained and used to improve the production strategy for this exotoxin, which is produced by Pseudomonas aeruginosa.
(via Semantic Scholar)
Sources: Crossref, NC State University Libraries
Added: July 21, 2021
1989 journal article
Characterization of hydrogen-uptake activity in the hyperthermophile Pyrodictium brockii.
Proceedings of the National Academy of Sciences, 86(1), 138–141.
MeSH headings : Archaea / growth & development; Archaea / metabolism; Bacteria / metabolism; Electron Transport; Hot Temperature; Hydrogen / metabolism; Hydrogenase / isolation & purification; Hydrogenase / metabolism; Kinetics
TL;DR:
The hydrogen-uptake activity of Pyrodictium brockii was localized predominantly in a particulate fraction, was reversibly inhibited by O2, and coupled H2 uptake to the reduction of positive potential artificial electron acceptors.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
14. Life Below Water
(OpenAlex)
Sources: Crossref, NC State University Libraries
Added: July 21, 2021
1989 journal article
Cultivation Techniques for Hyperthermophilic Archaebacteria: Continuous Culture of Pyrococcus furiosus at Temperatures near 100�C
Applied and Environmental Microbiology, 55(8), 2086–2088.
TL;DR:
A system which allows continuous cultivation of hyperthermophilic archaebacteria at temperatures approaching 100 degrees C has been developed and the resulting dilution rate and gas production profiles are discussed.
(via Semantic Scholar)
Sources: Crossref, NC State University Libraries
Added: July 21, 2021
1989 journal article
Growth and gas production for hyperthermophilic archaebacterium,Pyrococcus furiosus
Biotechnology and Bioengineering, 34(8), 1050–1057.
TL;DR:
An unstructured growth model was used to correlate growth and gas production for P. furiosus in complex seawater‐based media at 98° C both in the absence and presence of elemental sulfur.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
14. Life Below Water
(OpenAlex)
Sources: Crossref, NC State University Libraries
Added: August 7, 2021
1989 journal article
Probing coals for non-pyritic sulphur using sulphur-metabolizing mesophilic and hyperthermophilic bacteria
Fuel, 68(11), 1368–1375.
UN Sustainable Development Goal Categories
14. Life Below Water
(OpenAlex)
Sources: Crossref, NC State University Libraries
Added: August 7, 2021
1988 conference paper
Biological Sulfur Oxidation and Reduction for Coal Sulfur Speciation and Desulfurization
Proceedings of the 13th Annual EPRI Conference on Fuel Science and Conversion. Presented at the 13th Annual EPRI Conference on Fuel Science and Conversion, Santa Clara, CA.
Event: 13th Annual EPRI Conference on Fuel Science and Conversion at Santa Clara, CA
Source: NC State University Libraries
Added: September 18, 2021
1988 conference paper
Characterization of the H2-Uptake Hydrogenase Activity from the Extreme Thermophile Pyrodictium brockii
Presented at the First International IGT Symposium on Gas, Oil, and Coal Biotechnology, New Orleans, Louisiana.
Event: First International IGT Symposium on Gas, Oil, and Coal Biotechnology, at New Orleans, Louisiana on December 5-7, 1988
Source: NC State University Libraries
Added: July 27, 2021
1988 journal article
Destruction of Pharmaceutical and Biopharmaceutical Wastes by the Modar Supercritical Water Oxidation Process
Nature Biotechnology, 6(12), 1423–1427.
TL;DR:
A new technology for the destructive treatment of pharmaceutical wastes, the MODAR supercritical water oxidation process, that offers the advantages of almost complete destruction of organic matter coupled with a reasonable cost is described.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
6. Clean Water and Sanitation
(OpenAlex)
Sources: Crossref, NC State University Libraries
Added: July 21, 2021
1988 journal article
Effect of hydrogen and carbon dioxide partial pressures on growth and sulfide production of the extremely thermophilic archaebacterium Pyrodictium brockii
Biotechnology and Bioengineering, 32(4), 438–443.
TL;DR:
The results from this study indicate that a balance must be struck between cell yields and sulfide production in choosing an optimal hydrogen partial pressure for the growth of P. brockii.
(via Semantic Scholar)
Sources: Crossref, NC State University Libraries
Added: August 7, 2021
1988 journal article
Effect of hyperbaric oxygen and carbon dioxide on heterotrophic growth of the extreme thermophileSulfolobus acidocaldarius
Biotechnology and Bioengineering, 31(7), 750–754.
Sources: Crossref, NC State University Libraries
Added: August 7, 2021
1988 journal article
Engineering considerations for growth of bacteria at temperatures around 100°C
Applied Biochemistry and Biotechnology, 18(1), 53–73.
TL;DR:
Laboratory results from studies of three extremely thermophilic sulfur-dependent archaebacteria (Pyrodictium occultum, Pyrodictium brockii, andPyrococcus furiosus) are used to illustrate both metabolic characteristics and engineering challenges associated with bacteria growing around 100°C.
(via Semantic Scholar)
Sources: Crossref, NC State University Libraries
Added: July 21, 2021
1988 journal article
Extremely Thermophilic Archaebacteria: Biological and Engineering Considerations
Biotechnology Progress, 4(2), 47–62.
TL;DR:
Interdisciplinary interactions between molecular biologists, microbial ecologists, and biochemical engineers portend well for the biotechnological exploitation of novel extremely thermophilic methanogens and sulfur‐metabolizing archaebacteria.
(via Semantic Scholar)
Sources: Crossref, NC State University Libraries
Added: July 21, 2021
1988 journal article
Influence of hydrodynamics on physical and chemical gas absorption in packed columns
Industrial & Engineering Chemistry Research, 27(4), 636–642.
Sources: Crossref, NC State University Libraries
Added: August 7, 2021
1988 journal article
Significance of cell fluorescence color of acridine orange-stained Thiobacillus ferrooxidans under epifluoresence microscopy
Science and Technology Letters. Presented at the Biohydrometa Conference, Warwick.
Event: Biohydrometa Conference at Warwick
Source: NC State University Libraries
Added: September 18, 2021
1987 journal article
Development of a defined medium and two-step culturing method for improved exotoxin A yields from Pseudomonas aeruginosa
Applied and Environmental Microbiology, 53(9), 2013–2020.
TL;DR:
Northern blot analysis of total RNA isolates suggests that these effects on exotoxin A yields are based on events at the transcription level, and several divalent metallic cations (Ca2+, Cu2+, and Mn2+), in addition to iron, were found to inhibit the yield of exotoxins A.
(via Semantic Scholar)
Sources: Crossref, NC State University Libraries
Added: July 21, 2021
1987 journal article
Effect of Culturing Conditions on the Production of Exotoxin A by Pseudomonas aeruginosa
Annals of the New York Academy of Sciences, 506(1 Biochemical E), 663–668.
MeSH headings : ADP Ribose Transferases; Bacterial Toxins; Bacteriological Techniques; Biotechnology; Cations, Divalent; Cobalt / pharmacology; Copper / pharmacology; Culture Media; Exotoxins / biosynthesis; Ferrous Compounds / pharmacology; Magnesium / pharmacology; Manganese / pharmacology; Pentosyltransferases / metabolism; Pseudomonas aeruginosa / growth & development; Pseudomonas aeruginosa / metabolism; Virulence Factors
TL;DR:
It is shown that the presence of serum antibodies against exotoxin A protects human patients with P. aeruginosa infections and prevents the death of animals from a lethal challenge with this microorganism.
(via Semantic Scholar)
Sources: Crossref, NC State University Libraries
Added: August 7, 2021
1987 journal article
Growth of Extremely Thermophilic Archaebacteria under Elevated Hyperbaric Conditions
Annals of the New York Academy of Sciences, 506(1 Biochemical E), 51–66.
TL;DR:
The engineering problems that must be confronted include the development of bioreactors that can do the following: be operated under elevated pressures and temperatures, and under aerobic or anaerobic conditions; withstand the highly corrosive gases that are sometimes produced by these microorganisms.
(via Semantic Scholar)
Sources: Crossref, NC State University Libraries
Added: August 7, 2021
1987 journal article
Growth of the extreme thermophileSulfolobus acidocaldarius in a hyperbaric helium bioreactor
Biotechnology and Bioengineering, 29(9), 1066–1074.
TL;DR:
Results indicate that hyperbaric helium bioreactors will be important in the study of extremely thermophilic bacteria that are isolated from pressurized environments.
(via Semantic Scholar)
Sources: Crossref, NC State University Libraries
Added: July 21, 2021
1987 journal article
Sulfur Reduction by the Extremely Thermophilic Archaebacterium Pyrodictium occultum
Applied and Environmental Microbiology, 53(7), 1690–1693.
TL;DR:
The relationship between growth and biological sulfur reduction for the extremely thermophilic archaebacterium Pyrodictium occultum was studied and the addition of yeast extract to the medium was found to increase hydrogen sulfide production significantly.
(via Semantic Scholar)
Sources: Crossref, NC State University Libraries
Added: August 7, 2021
1987 journal article
Use of epifluorescence microscopy for characterizing the activity ofThiobacillus Ferrooxidans on iron pyrite
Biotechnology and Bioengineering, 30(1), 138–146.
TL;DR:
The work presented here suggests that epifluorescence microscopy using acridine orange can be a useful probe to study acidophilic metal‐leaching bacteria and resolves the controversy concerning the significance of attachment during the leaching process.
(via Semantic Scholar)
Sources: Crossref, NC State University Libraries
Added: July 21, 2021
1986 journal article
Experimental methods for measuring static liquid holdup in packed columns
AIChE Journal, 32(11), 1920–1923.
Sources: Crossref, NC State University Libraries
Added: July 21, 2021
1986 journal article
Hollow Fiber Microfiltration Methods for Recovery of Rat Basophilic Leukemia Cells (RBL-2H3) From Tissue Culture Media
Biotechnology Progress, 2(4), 230–233.
TL;DR:
The purpose here is to discuss some operating experience with the use of hollow fiber tangential flow filters for recovery of rat basophilic leukemia cells (RBG2H3) grown in suspension culture, which have histamine-containing granules and receptors for IgE and have been used as a model to study the IgEreceptor interaction.
(via Semantic Scholar)
Sources: Crossref, NC State University Libraries
Added: July 21, 2021
1986 journal article
Microbiological Metal Transformations: Biotechnological Applications and Potential
Biotechnology Progress, 2(1), 1–15.
TL;DR:
Developpements recents concernant la biolixiviation des metaux, the recuperation and the valorisation des minerais, considerations d'ingenierie applicable and perspectives.
(via Semantic Scholar)
Sources: Crossref, NC State University Libraries
Added: July 21, 2021
1985 journal article
Conditioning Coal Gas with Refrigerated Methanol in a System of Packed Columns
Chemical Engineering Communications, 34(1-6), 27–35.
UN Sustainable Development Goal Categories
12. Responsible Consumption and Production
(Web of Science)
Sources: Crossref, NC State University Libraries
Added: August 7, 2021
1984 journal article
Design of packed, adiabatic absorbers: physical absorption of acid gases in methanol
Industrial & Engineering Chemistry Process Design and Development, 23(1), 102–109.
UN Sustainable Development Goal Categories
12. Responsible Consumption and Production
(Web of Science)
Sources: Crossref, NC State University Libraries
Added: July 21, 2021
1983 report
Coal Gasification/Gas Cleanup Test Facility. Volume 4. A Mathematical Model of the Packed Column Acid Gas Absorber
(No. EPA/600/7-83/053). Washington, D.C.: U.S. Environmental Protection Agency.
Source: NC State University Libraries
Added: September 18, 2021
1982 report
Coal Gasification/Gas Cleanup Test Facility, Vol III: Environmental Assessment of Operation with New Mexico Subbituminous Coal and Chilled Methanol
(No. EPA-600/7-82-054). Washington, D.C.: U.S. Environmental Protection Agency.
Source: NC State University Libraries
Added: September 18, 2021
1982 report
Coal Gasification/Gas Cleanup Test Facility: Vol II: Environmental Assessment of Operation with Devolatilized Bituminous Coal and Chilled Methanol
(No. EPA-600/7-82-023). Washington, D.C.: U.S. Environmental Protection Agency.
Source: NC State University Libraries
Added: September 18, 2021
1981 journal article
Physical Absorption of CO2and Sulfur Gases from Coal Gasification: Simulation and Experimental Results
Separation Science and Technology, 16(10), 1389–1414.
UN Sustainable Development Goal Categories
12. Responsible Consumption and Production
(Web of Science)
Sources: Crossref, NC State University Libraries
Added: August 7, 2021
1980 report
Coal Gasification/Gas Cleanup Test Facility: Vol I: Description and Operations
(No. EPA-600/7-80-046a). Washington, D.C.: U.S. Environmental Protection Agency.
Source: NC State University Libraries
Added: September 18, 2021
1980 journal article
How clean gas is made from coal
Environmental Science & Technology, 14(6), 658–666.
TL;DR:
The overall objective of the project is to characterize completely the gaseous and condensed-phase emissions from the gasification-gascleaning process, and to determine how emission rates of various pollutants and methanation catalyst poisons depend on adjustable process parameters.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
7. Affordable and Clean Energy
(Web of Science)
Sources: Crossref, NC State University Libraries
Added: July 21, 2021
1977 journal article
Electrochemical regeneration of NAD+on carbon electrodes
Biotechnology and Bioengineering, 19(8), 1215–1218.
Sources: Crossref, NC State University Libraries
Added: July 21, 2021
1976 journal article
Steroids and oxygen solubility
Steroids, 28(3), 307–310.
MeSH headings : Betamethasone / blood; Cortisone / blood; Dexamethasone / blood; Humans; Methods; Oxygen / blood; Prednisolone / blood; Solubility
TL;DR:
Determinations of oxygen solubility in serum were made utilizing various concentrations of corticosteroids in the serum, and within a certain concentration range dexamethasone and betameth asone caused an increase in oxygensolubility.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
6. Clean Water and Sanitation
(OpenAlex)
Sources: Crossref, NC State University Libraries
Added: July 21, 2021
journal article
Carbohydrate and lignin are simultaneously solubilized from unpretreated switchgrass by microbial action at high temperature
Kataeva, I., Foston, M. B., Yang, S. J., Pattathil, S., Biswal, A. K., Poole, F. L., … York, W. S. Energy & Environmental Science, 6(7), 2186–2195.
Source: NC State University Libraries
Added: August 6, 2018
journal article
Characterization of extremely thermostable enzymatic breakers (alpha-1,6-galactosidase and beta-1,4-mannanase) from the hyperthermophilic bacterium Thermotoga neapolitana 5068 for hydrolysis of guar gum
McCutchen, C. M., Duffaud, G. D., Leduc, P., Petersen, A. R. H., Tayal, A., Khan, S. A., & Kelly, R. M. Biotechnology and Bioengineering, 52(2), 332–339.
Source: NC State University Libraries
Added: August 6, 2018
journal article
Complete genome sequences for the anaerobic, extremely thermophilic plant biomass-degrading bacteria caldicellulosiruptor hydrothermalis, caldicellulosiruptor kristjanssonii, caldicellulosiruptor kronotskyensis, caldicellulosiruptor owensensis, and caldic
Blumer-Schuette, S. E., Ozdemir, I., Mistry, D., Lucas, S., Lapidus, A., Cheng, J. F., … Walston-Davenport, K. Journal of Bacteriology, 193(6), 1483–1484.
Source: NC State University Libraries
Added: August 6, 2018
journal article
Lignocellulosic biomass deconstruction by the extremely thermophilic genus caldicellulosiruptor
Conway, J. M., Zurawski, J. V., Lee, L. L., Blumer-Schuette, S. E., & Kelly, R. M. Thermophilic Microorganisms, 91–119.
Source: NC State University Libraries
Added: August 6, 2018
journal article
Viscosity reduction of hydraulic fracturing fluids through enzymatic hydrolysis
Tayal, A., Kelly, R. M., & Khan, S. A. Society of Petroleum Engineers Journal, 2(2), 204–212.
Source: NC State University Libraries
Added: August 6, 2018
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