@article{toomer_maharjan_harding_vu_malheiros_mian_joseph_read_oviedo-rondon_anderson_2024, title={Effects of full-fat high-oleic soybean meal in layer diets on performance, egg quality and chemical composition}, volume={103}, ISSN={["1525-3171"]}, url={https://doi.org/10.1016/j.psj.2024.104074}, DOI={10.1016/j.psj.2024.104074}, abstractNote={The utilization of full-fat high-oleic soybean meal in layer diets could lead to value-added poultry products. To test this idea, 336 hens were randomly assigned to 4 isonitrogenous (18.5% CP) and isocaloric (2,927 kcal/kg) formulated diets and fed the following diets for eight weeks: conventional control solvent-extracted defatted soybean meal (CON); extruded-expelled defatted soybean meal (EENO); full fat normal-oleic soybean meal (FFNO); or full fat high-oleic soybean meal (FFHO). Body weights (BW) were collected at week 0 and week 8. Eggs were collected daily, and the totals counted each week. Feed consumption was measured weekly, and egg quality was measured bi-weekly. Eggs were collected at wk 0 and wk 8 for fatty acid analysis. There were no significant treatment differences in any of the production parameters measured, BW, feed consumption, feed conversion ratio or egg production (P > 0.05). Eggshell strength was significantly greater in eggs produced from the EENO group as compared to the control (P < 0.01), while egg yolk color was significantly darker in eggs of the control and EENO treatment groups relative to the FFNO and FFHO treatments (P < 0.0001). Eggs produced by hens fed the FFHO diet had a 52% increase in monounsaturated n-9 oleic acid content (P < 0.0001) and reduced palmitic (P < 0.01) and stearic (P < 0.0001) saturated fatty acid levels as compared to the conventional controls. These results validate the utilization of FFHO as a value-added poultry feed ingredient to enrich the eggs and/or poultry meat produced.}, number={10}, journal={POULTRY SCIENCE}, author={Toomer, Ondulla T. and Maharjan, Pramir and Harding, Kari L and Vu, Thien Chuong and Malheiros, Ramon and Mian, Rouf and Joseph, Michael and Read, Quentin D. and Oviedo-Rondon, Edgar Orlando and Anderson, Kenneth E.}, year={2024}, month={Oct} }
 @article{ali_joseph_alfaro-wisaquillo_quintana-ospina_patino_vu_dean_fallen_mian_taliercio_et al._2024, title={Effects of high oleic full-fat soybean meal on broiler live performance, carcass and parts yield, and fatty acid composition of breast fillets}, volume={103}, ISSN={["1525-3171"]}, DOI={10.1016/j.psj.2023.103399}, abstractNote={The effects of high oleic oil full-fat (HO-FF) soybean meal (SBM) on broiler meat quality could lead to value-added food products. This experiment evaluated the effects of dietary normal oleic extruded expelled (NO-EE), normal oleic full-fat (NO-FF), or HO-FF SBM on live performance, carcass and parts yield, and breast fatty acid composition. Diets were formulated to be isoenergetic and isonitrogenous. A total of 540 Ross-708 male broilers were raised on floor pens with 18 broilers/pen and ten replicates/treatment. Data were analyzed in a completely randomized design. Chickens were fed with a starter (0 – 14 d), grower (15 – 35 d), or a finisher diet (36 – 47 d) up to 47 d. Chickens were weighed at 7, 14, 35, and 47 d. At 48 d, four broilers per pen were processed. Breast samples were collected and evaluated for quality and fatty acid content. Broilers fed diets with NO-EE were heavier (P < 0.05) than chickens fed diets with full-fat SBM (NO-FF and HO-FF) at d 7, 14, 35 while feed conversion ratio (FCR) of NO-EE was best (P < 0.05) at 7 d and 47 d. Carcass yield was also higher for broilers fed NO-EE than the other treatments. Diet did not affect parts yield, breast meat color, cooking, drip loss, white stripping, or SM quality parameters. More breast fillets without wooden breast (score 1) were observed (P < 0.05) for NO-FF than the other two treatments. The breast meat fatty acid profile (g fatty acid/ 100 g of all fatty acids) was significantly affected (P < 0.001) by diet. Broilers fed the HO-FF SBM diet had 54 to 86% more oleic acid, 72.5% to 2.2 times less linoleic acid, and reduced stearic and palmitic acid levels in the breast meat than NO-FF and NO-EE. In conclusion, feeding HO-FF to broilers enriched the oleic acid content of their breast meat while reducing the saturated fatty acid content relative to the NO-FF and NO-EE treatment groups.}, number={3}, journal={POULTRY SCIENCE}, author={Ali, Muhammad and Joseph, Michael and Alfaro-Wisaquillo, Maria Camila and Quintana-Ospina, Gustavo Adolfo and Patino, Danny and Vu, Thien and Dean, Lisa L. and Fallen, Ben and Mian, Rouf and Taliercio, Earl and et al.}, year={2024}, month={Mar} }
 @misc{toomer_oviedo-rondon_ali_joseph_vu_fallen_mian_2024, title={Full-Fat Soybean Meals as an Alternative Poultry Feed Ingredient-Feed Processing Methods and Utilization-Review and Perspective}, volume={14}, ISSN={["2076-2615"]}, DOI={10.3390/ani14162366}, abstractNote={On a global scale, the poultry industry expands its wings in terms of meat and egg production to the masses. However, this industry itself requires a sustainable and permanent supply of different inputs, one of which is poultry feed and nutrition. Soybean is a versatile protein that is offered to poultry in different inclusion rates in commercial diets after being processed using various thermal and mechanical processing methods. Conventional commercial soybean meal is usually prepared by the extraction of oil from whole soybeans using solvents, producing a meal that has approximately 1% crude fat. Without oil extraction, full-fat soybean (FFSBM) is produced, and it is an excellent source of dietary energy and protein for poultry with a nutritional profile of 38-40% protein and 18-20% crude fat, on average. FFSBM has less crude protein (CP) than solvent-extracted soybean meal (SE SBM) but higher metabolizable energy due to higher fat content. Alternatively, extruded expeller processing produces defatted soybean meal containing approximately 6-7% crude fat. Studies have demonstrated that FFSBM can be used in poultry diets to improve poultry nutrition, performance, and quality of the poultry meat and eggs produced. This review aims to evaluate the nutrition and use of meals prepared from conventional and high-oleic soybeans using various feed processing methods.}, number={16}, journal={ANIMALS}, author={Toomer, Ondulla T. and Oviedo-Rondon, Edgar Orlando and Ali, Muhammad and Joseph, Michael and Vu, Thien and Fallen, Ben and Mian, Rouf}, year={2024}, month={Aug} }
 @article{patino_toomer_vu_oviedo-rondon_mian_frinsko_hall_kelly_mann_joseph_2024, title={Influence of extruded soybean meal with different fat contents and varying oleic acid content on floating fish feed quality and composition}, volume={317}, ISSN={["1873-2216"]}, DOI={10.1016/j.anifeedsci.2024.116088}, abstractNote={Soybean meal (SBM) is the most widely used source of high-quality plant protein within the feed industry. Raw soybeans are conventionally processed to reduce antinutritional factors, enhance protein bioavailability and improve the overall quality of the final feed product. New high-oleic (HO) cultivars with enhanced unsaturated fatty acids are being utilized in the production of HO SBM for use in the animal feed industry. However, no studies to date have examined the impact of HO SBM on feed formulation and processing of aquaculture feeds. Therefore, we aimed to determine the quality of feed for juvenile domesticated striped bass (Morone saxatilis) using SBM prepared from HO or normal-oleic (NO) soybeans and extrusion-expeller processing. The following four soybean meals were used in our experimental diets: solvent-extracted defatted normal oleic (SENO), full-fat normal oleic (FFNO), extruded-expelled defatted normal oleic (EENO), or full-fat high oleic (FFHO). These meals replaced half of the fishmeal (FM) normally included in a nutritionally complete marine finfish diet. Physico-chemical feed variables and chemical composition were determined during feed production. Data were analyzed using one-way ANOVA and means were separated using Tukey's t-test. The specific mechanical energy of the diet was reduced with the addition of full-fat SBMs during the extrusion process. All finished fish diets were of similar high quality with high pellet durability index and protein content (P > 0.05). This suggested that using different types SBM, including high oleic, to replace 50 % of the fish meal in a floating feed for juvenile domesticated striped bass does not adversely affect the feed quality or nutritional content.}, journal={ANIMAL FEED SCIENCE AND TECHNOLOGY}, author={Patino, Danny B. and Toomer, Ondulla T. and Vu, Thien C. and Oviedo-Rondon, Edgar O. and Mian, Rouf and Frinsko, Mike and Hall, Steven and Kelly, Ryan and Mann, Jason and Joseph, Michael}, year={2024}, month={Nov} }
 @article{mian_cunicelli_carter jr_villagarcia_fallen_2024, title={Registration of USDA-N6006 soybean germplasm combining high yield, flood tolerance, and elevated oil content}, ISSN={["1940-3496"]}, DOI={10.1002/plr2.20358}, abstractNote={AbstractUSDA‐N6006 soybean [Glycine max (L.) Merr.] (Reg. no. GP‐527, PI 704140) is a conventional F4–derived early maturity group (MG) VI germplasm jointly released by USDA‐ARS and North Carolina Agricultural Research Service in 2023. USDA‐N6006 is the first MG VI, publicly released germplasm that combines flood tolerance with high‐yield lodging resistance, and elevated seed oil. Over 19 environments of the MG VI USDA Southern Uniform Soybean Trials (2017–2018), seed yield of USDA‐N6006 (3393 kg ha−1) was similar to high‐yielding lodging‐resistant check cultivars ‘NC‐Dunphy’ (3427 kg ha−1) and ‘NC‐Dilday’ (3475 kg ha−1). It matured 1 day earlier than NC‐Dunphy and exhibited similar lodging scores. Over 5 years of testing in North Carolina (2016–2019), USDA‐N6006 exhibited very robust flood tolerance. Mean flooding scores were numerically or statistically (p < 0.05) superior to the four flood‐tolerant controls at both V4 and R1 growth stages. USDA‐N6006 was not intentionally bred to be flood tolerant and identified as such only through an extensive screening of “in house” USDA breeding lines. USDA‐N6006 and most of its antecedents are unique in that they are derived from unreleased breeding lines developed at the flood prone Tidewater Research station in North Carolina. Inadvertent selection for flood tolerance may have been a part of the USDA's long‐term breeding effort to improve yield. Seed oil content of USDA‐N6006 (237 g kg−1) was similar to that of elevated seed oil cultivar NC‐Dilday, but significantly higher than NC‐Dunphy (228 g kg−1) in the Uniform Trials. Seed protein content and estimated meal protein contents of USDA‐N6006 (389 g kg−1 and 46.4%, respectively) were similar to that of NC‐Dunphy. USDA‐N6006 should be excellent parental stock for applied breeding in the southern United States.}, journal={JOURNAL OF PLANT REGISTRATIONS}, author={Mian, M. A. Rouf and Cunicelli, Mia J. and Carter Jr, Thomas E. and Villagarcia, Margarita and Fallen, Benjamin D.}, year={2024}, month={Feb} }
 @article{fallen_robertson_taliercio_mian_carter jr_2024, title={Registration of high-yielding, high-protein soybean germplasm USDA-N7007 derived from wild soybean PI 366122}, ISSN={["1940-3496"]}, DOI={10.1002/plr2.20383}, abstractNote={Abstract USDA‐N7007 is a non‐GM, maturity group (MG) VII soybean [ Glycine max (L.) Merr.] (Reg. no. GP‐529, PI 705147) germplasm released by the USDA Agricultural Research Service in conjunction with the North Carolina Agricultural Research Service in December of 2023. USDA‐N7007 is a high‐yielding, high‐protein germplasm derived from wild soybean ( Glycine soja Siebold & Zucc; PI 366122) and small‐seeded MG VII USDA cultivar N7103. Over 47 combined testing environments of the USDA Southern Uniform and USB Protein Diversity Tests (2018–2021), USDA‐N7007 yielded 98% of the check mean and 102% of the test mean. The average protein content of USDA‐N7007 was significantly higher (432 g kg −1 ) than the average check means of 402 g kg −1 and 413 g kg −1 in the USDA Southern Uniform and USB Protein Diversity Tests, respectively. Across both tests (2019–2021), the release was significantly ( p < 0.05) higher in protein (+7 g kg −1 ), with 9% higher seed yield (+242 kg ha −1 ) than the recurrent parent N7103. USDA‐N7007 is resistant to lodging, southern root‐knot nematode, and stem canker. To the best of our knowledge, this is the first US release clearly demonstrating that the wild soybean genome can be incorporated into an elite cultivar to increase seed protein without a negative effect on seed yield. This release is a truly novel and valuable resource for development of future US soybean cultivars because it will be useful to improve both genetic diversity and seed protein simultaneously without a negative effect on seed yield.}, journal={JOURNAL OF PLANT REGISTRATIONS}, author={Fallen, Benjamin and Robertson, Marta and Taliercio, Earl and Mian, M. A. Rouf and Carter Jr, Thomas E.}, year={2024}, month={Jul} }
 @article{huynh_van_mian_mchale_2024, title={Single- and multiple-trait quantitative trait locus analyses for seed oil and protein contents of soybean populations with advanced breeding line background}, volume={44}, ISSN={["1572-9788"]}, DOI={10.1007/s11032-024-01489-2}, abstractNote={Soybean seed oil and protein contents are negatively correlated, posing challenges to enhance both traits simultaneously. Previous studies have identified numerous oil and protein QTLs via single-trait QTL analysis. Multiple-trait QTL methods were shown to be superior but have not been applied to seed oil and protein contents. Our study aimed to evaluate the effectiveness of single- and multiple-trait multiple interval mapping (ST-MIM and MT-MIM, respectively) for these traits using three recombinant inbred line populations from advanced breeding line crosses tested in four environments. Using original and simulated data, we found that MT-MIM did not outperform ST-MIM for our traits with high heritability (H}, number={8}, journal={MOLECULAR BREEDING}, author={Huynh, Tu and Van, Kyujung and Mian, M. A. Rouf and Mchale, Leah K.}, year={2024}, month={Aug} }
 @misc{toomer_oviedo_ali_patino_joseph_frinsko_vu_maharjan_fallen_mian_2023, title={Current Agronomic Practices, Harvest & Post-Harvest Processing of Soybeans (Glycine max)-A Review}, volume={13}, ISSN={["2073-4395"]}, DOI={10.3390/agronomy13020427}, abstractNote={Globally, soybeans are grown to meet the needs for animal and human nutrition, oil extraction, and use in multiple industrial applications. Decades of soybean research, innovative farming methods, and the use of higher yielding resistant seed varieties have led to increased crop yields. Globally, soybean producers have utilized enhanced processing methods to produce nutritious high-quality meal and extracted oil for use in animal feed and within the food industry. Soybeans contain highly digestible proteins and are processed using various mechanical and chemical techniques to produce high quality animal feed ingredients. Defatted soybean meal (DSM) is usually prepared by the solvent extraction process of soybeans, whereby almost all oil content is removed. When oil is not extracted, full-fat soybean meal (FFSBM) is created. This form provides an excellent source of dietary energy by retaining the lipid component and is very useful in animal feeds by reducing the need for adding exogeneous lipids. However, some anti-nutritional factors (ANF) are present in FFSBM if not properly heat treated before inclusion in the finished feed. These ANF adversely affect the internal organ function and overall growth performance of the animal. Among these ANF, protease inhibitors are most important, but can be readily destroyed with optimal thermal processing. However, if the process protocols are not followed precisely, excessive heat treatment may occur, resulting in both reduced protein quality and amino acid bioavailability in the meal. Conversely, insufficient heat treatment may result in the retention of some ANF in the meal. Thermally resistant ANF can be greatly reduced in the bean and meal when dietary enzyme supplementation is included in the finished feed. This approach is cost-effective and most commonly utilized commercially. After processing, the soybean meal quality is often measured using in vitro methods performed at commercial analytical laboratories to assess the nitrogen solubility index (NSI), protein dispersibility index (PDI), urease activity (UA), and protein solubility in potassium hydroxide. Once properly processed, FFSBM or DSM can be utilized optimally in the diets of poultry and aquaculture to enhance the economic viability, animal nutrition, production performance, and the quality and nutritional value of the meat and/or eggs produced.}, number={2}, journal={AGRONOMY-BASEL}, author={Toomer, Ondulla T. and Oviedo, Edgar O. and Ali, Muhammad and Patino, Danny and Joseph, Michael and Frinsko, Mike and Vu, Thien and Maharjan, Pramir and Fallen, Ben and Mian, Rouf}, year={2023}, month={Feb} }
 @article{maharjan_rahimi_harding_vu_malheiros_oviedo-rondon_mian_joseph_dean_anderson_et al._2023, title={Effects of full-fat high-oleic soybean meal in layer diets on nutrient digestibility and egg quality parameters of a white laying hen strain}, volume={102}, ISSN={["1525-3171"]}, url={https://doi.org/10.1016/j.psj.2023.102486}, DOI={10.1016/j.psj.2023.102486}, abstractNote={This study was conducted to understand the impact of including full fat high-oleic soybean meal in layer hen diets on nutrient digestibility and added nutritional value in eggs. Forty-eight layers (∼36 wk old) were randomly assigned to one of 4 isonitrogenous (18.5% crude protein) treatment diets with 12 replicate birds per treatment in a 3-wk study. Treatments were 1) solvent extracted defatted soybean meal + corn diet, 2) dry extruded defatted soybean meal + corn, 3) full-fat soybean meal + corn, 4) high-oleic full-fat soybean meal + corn diet. Apparent ileal digestibility of crude fat (CF) and crude protein (CP) were determined using celite (∼2%) as an indigestible marker. Tibia strength and egg quality parameters (egg weight, shell strength, Haugh unit, shell color, and yolk color) were recorded during the study. Fatty acid profiles, including the monounsaturated fatty acid, oleic acid (C18:1, cis), in eggs and adipogenic tissue (liver, muscle, and fat pad) were measured using gas chromatography (GC-FID). Digestibility values of CF ranged from 71 to 84% and CP varied from 67 to 72% for treatment diets, with treatment mean values being no different (P > 0.05) between treatment diets. No differences between treatment diets in tibia strength or egg quality parameters (egg weight, shell strength, and Haugh unit) were observed (P > 0.05) except for yolk color. Similarly, there were no differences in the total lipids in egg yolk (P > 0.05) between treatment diets. However, oleic acid percentage of total lipid in egg and tissue was significantly higher (P < 0.001) in hens given the high-oleic full-fat soybean meal diet than in other treatment groups. No difference was observed in oleic acid percentage of total lipid in egg between the other 3 treatment diets (P > 0.05). Overall, the results exhibited that the eggs and tissue of layer hens fed the full-fat high-oleic acid soybean meal diet were higher in oleic acid while the CF and CP digestibility remained similar to the digestibility of the other diets.}, number={4}, journal={POULTRY SCIENCE}, author={Maharjan, Pramir and Rahimi, Amin and Harding, Kari L and Vu, Thien Chuong and Malheiros, Ramon and Oviedo-Rondon, Edgar O. and Mian, Rouf and Joseph, Michael and Dean, Lisa and Anderson, Kenneth E. and et al.}, year={2023}, month={Apr} }
 @article{gillenwater_mian_cunicelli_mcneece_taliercio_2023, title={Identification of High-Yielding Soybean Lines with Exceptional Seed Composition Qualities}, volume={3}, ISSN={["2673-7655"]}, DOI={10.3390/crops3040029}, abstractNote={In current markets, the primary uses for soybean seeds are in products derived from their oil or protein content. However, growers are compensated based on seed yield, so a more valuable crop is one that does not compromise on yield when compared with existing options, with an optimum combination of protein and oil. A negative correlation of seed protein with seed yield and oil makes the simultaneous improvement of these traits difficult but not impossible through conventional breeding. Selections of lines with exceptional yield and seed composition were made from two recombinant inbred line (RIL) soybean mapping populations to identify high protein and/or high oil lines with yields comparable to elite cultivars. The performance of these RILs was evaluated in multiple environments, and several genotypes were identified with yields comparable to those of high-yielding check cultivars with seed protein and/or oil content superior to the checks. These genotypes will provide breeders with additional sources of germplasm for continuing efforts to improve seed composition traits without compromising seed yield and provide growers with more profitable cultivars.}, number={4}, journal={CROPS}, author={Gillenwater, Jay and Mian, Rouf and Cunicelli, Mia and McNeece, Brant and Taliercio, Earl}, year={2023}, month={Dec}, pages={333–342} }
 @article{ali_joseph_alfaro-wisaquillo_quintana-ospina_penuela-sierra_patino_vu_mian_toomer_oviedo-rondon_2024, title={Influence of extruded soybean meal with varying fat and oleic acid content on nitrogen-corrected apparent metabolizable energy in broilers}, volume={103}, ISSN={["1525-3171"]}, DOI={10.1016/j.psj.2023.103408}, abstractNote={High oleic (HO) soybeans may serve as a value-added feed ingredient; providing amino acids and estimating their dietary energy value for broilers is essential. In this study, we determined the apparent metabolizable energy (AME), AME corrected for zero nitrogen retention (AMEn), digestibility, and nitrogen (N) retention of HO full-fat (HO-FF) soybean as compared to solvent-extracted soybean meal (SE-SBM), normal oleic full-fat (NO-FF) and extruded expeller (NO-EE) soybean. A total of 240 Ross-708 male broilers were selected, with eight replicates per treatment and six chicks per cage. The AME and AMEn were estimated using the difference method with a 30% inclusion of test ingredients using a corn-soy reference diet with partial and total excreta collection. The index method with partial excreta collection used titanium dioxide as an inert marker. The same starter diet was provided for all birds for 14 days, followed by the reference and assay diets for the next six adaptation days. Total excreta was collected twice a day for three days. The AME and AMEn values determined for the HO-FF and NO-FF were higher (P < 0.001) than the NO-EE and SE-SBM. The AME of SE-SBM and NO-EE were similar with both methods, but the AMEn of SE-SBM was lower than the NO-EE only with the partial collection method. The agreement between AME and AMEn values determined by partial and total excreta collection analysis was 98%. Data from the total excreta collection method yielded higher AME and AMEn values (P < 0.001) than those from the partial collection method. In summary, HO-FF and NO-FF soybean meals had similar AME and AMEn values. The HO-FF soybean had 39 and 24% higher AME and AMEn than SE-SBM. Hence, high oleic full-fat soybean meal could serve as a valuable alternative feed ingredient to conventional SE-SBM meals in broiler diets, providing additional energy while providing amino acids and more oleic acid to enrich poultry meat products.}, number={3}, journal={POULTRY SCIENCE}, author={Ali, Muhammad and Joseph, Michael and Alfaro-Wisaquillo, Maria Camila and Quintana-Ospina, Gustavo Adolfo and Penuela-Sierra, Lina -Maria and Patino, Danny and Vu, Thien and Mian, Rouf and Toomer, Ondula and Oviedo-Rondon, Edgar Orlando}, year={2024}, month={Mar} }
 @article{fallen_mian_robertson_powell_carter jr_2023, title={Registration of USDA-N7006 soybean germplasm with increased tolerance to drought stress and 37.5% pedigree from Asian accessions PI 416937 and PI 407859-2}, volume={17}, ISSN={["1940-3496"]}, DOI={10.1002/plr2.20323}, abstractNote={AbstractUSDA‐N7006 is a conventional (non‐genetically modified) maturity group (MG) VII soybean [Glycine max (L.) Merr.] (Reg. no. GP‐525 , PI 702948) germplasm released jointly by the USDA‐ARS and the North Carolina Agricultural Research Service in January of 2023. USDA‐N7006 was released because of its drought tolerance, exotic parentage (37.5%), and high yield potential. USDA‐N7006 traces 12.5% of its parentage to PI 416937 from Japan and 25% to PI 407859‐2 from South Korea. USDA‐N7006 is the first North American soybean release derived from PI 407859‐2. USDA‐N7006 is an F4‐derived germplasm from the hybridization of USDA breeding lines TCPR01‐83 and N01‐11136. From 2015 to 2021, USDA‐N7006 was evaluated in 57 environments across the Southeast in the United Soybean Board (USB) Protein and Diversity MG VII Tests and the USDA Southern Soybean Preliminary and Uniform MG VII Tests. In the MG VII USDA and USB Protein and Diversity Tests, USDA‐N7006 yielded 96% and 98% of the check mean, respectively. Over six droughted environments in North Carolina and South Carolina, USDA‐N7006 exhibited less wilting (greater drought tolerance) than ‘USDA‐N8002’, a previously released cultivar exhibiting drought tolerance. The unique pedigree, delayed canopy wilting under drought stress, and high yield of USDA‐N7006 make it an excellent candidate for parental stock for soybean breeding programs aiming to improve drought tolerance.}, number={3}, journal={JOURNAL OF PLANT REGISTRATIONS}, author={Fallen, Benjamin D. and Mian, M. A. Rouf and Robertson, Marta H. and Powell, Emily and Carter Jr, Thomas E.}, year={2023}, month={Sep}, pages={573–579} }
 @article{mian_cunicelli_carter jr_villagarcia_fallen_2023, title={Registration of high-yielding maturity group V germplasm USDA-N5001 with high seed and meal protein contents}, ISSN={["1940-3496"]}, DOI={10.1002/plr2.20306}, abstractNote={AbstractUSDA‐N5001 (Reg. no. GP‐524, PI 702592) is a conventional early maturity group (MG) V soybean [Glycine max (L.) Merr.] germplasm with high yield potential and elevated seed and meal protein contents. USDA‐N5001 was jointly released as a non–genetically modified germplasm by the USDA‐ARS and the North Carolina Agricultural Research Service in January 2023. USDA‐N5001, experimental name N16‐590, was derived from the hybridization of high‐yielding breeding line N08‐145 and high‐protein breeding line Pro5‐1. Across 22 environments of the 2019 and 2020 USDA Uniform Soybean Trials Southern States, USDA‐N5001 yielded 105%, 98%, and 102% of the three checks ‘Ellis’, ‘AG55X’, and ‘TN11‐5140’, respectively. On a zero‐moisture basis, its protein content (425 g kg−1) was significantly higher (p < 0.05) than those of the checks (399–402 g kg−1). The meal protein content of USDA‐N5001 (49.4%) was also significantly higher (p < 0.05) than the checks (46.7%–46.9%). The new release matured 2 days and 1 day later than Ellis and AG55X, respectively, but 5 days earlier than TN11‐5140. USDA‐N5001 is the first high‐yielding, high‐seed‐ and meal‐protein MG V‐Early (relative maturity 5.3) line developed by USDA‐ARS in North Carolina. With the recent increases in production of MG V soybeans in the southeastern United States and the national focus on higher‐meal protein lines, USDA‐N5001 should be highly useful to soybean breeders and growers of conventional soybeans in the southeastern United States.}, journal={JOURNAL OF PLANT REGISTRATIONS}, author={Mian, M. A. Rouf and Cunicelli, Mia J. and Carter Jr, Thomas E. E. and Villagarcia, Margarita and Fallen, Benjamin D.}, year={2023}, month={Jul} }
 @article{ali_joseph_alfaro-wisaquillo_quintana-ospina_patino_penuela-sierra_vu_mian_taliercio_toomer_et al._2023, title={Standardized ileal amino acid digestibility of high-oleic full-fat soybean meal in broilers}, volume={102}, ISSN={["1525-3171"]}, DOI={10.1016/j.psj.2023.103152}, abstractNote={High-oleic (HO) soybean may serve as a value-added feed ingredient to enrich poultry meat due to its fatty acid content. However, the amino acid (AA) nutrient digestibility of soybean meal (SBM) made from these soybeans has yet to be determined. The objective of this study was to determine apparent ileal AA digestibility (AID) and standardized ileal AA digestibility (SID) of high-oleic full-fat (HO-FF) SBM compared to normal oleic full-fat (NO-FF), normal oleic extruded expeller (NO-EE), and solvent-extracted SBM (SE-SBM) in broilers. A nitrogen-free basal diet (NFD) was fed to 1 treatment group with 10 chicks/cage to determine basal endogenous losses (BEL). Titanium dioxide was used as an inert marker. The test diets contained 57.5% of the basal NFD and 42.5% of 1 of the 4 soybean sources. A total of 272 Ross-708 male broilers were placed in 40 battery cages with 5 treatments and 8 replicates per treatment. A common starter diet was provided to all the chickens for 14 d. Experimental diets were provided as a mash for 9 d before sample collection. Chickens were euthanized with CO2 on d 23, and contents of the distal ileum were collected, frozen, and freeze-dried. The BEL were similar to the values found in the literature. At d 23, broilers fed the SE-SBM had the highest body weight gain and best FCR compared to chickens fed the HO-FF and NO-FF treatments (P < 0.001). Broilers fed the SE-SBM and NO-EE experimental diets had (P < 0.001) higher apparent ileal AA digestibility and AA SID than broilers fed the HO-FF and NO-FF treatments. In conclusion, the SID of AA from HO-FF is similar to the digestibilities of other full-fat soybeans found in the literature and is lower than that of NO-EE and SE-SBM.}, number={12}, journal={POULTRY SCIENCE}, author={Ali, Muhammad and Joseph, Michael and Alfaro-Wisaquillo, Maria Camila and Quintana-Ospina, Gustavo Adolfo and Patino, Danny and Penuela-Sierra, Lina-Maria and Vu, Thien and Mian, Rouf and Taliercio, Earl and Toomer, Ondulla and et al.}, year={2023}, month={Dec} }
 @article{mcneece_gillenwater_li_mian_2021, title={Assessment of soybean test weight among genotypes, environments, agronomic and seed compositional traits}, ISSN={["1435-0645"]}, DOI={10.1002/agj2.20665}, abstractNote={AbstractThe value of soybean [Glycine max (L.) Merr.] depends on quantity (yield) and quality, such as seed composition and test weight (TW). Test weight is a bulk density measurement for grain quality evaluation, and higher TW grains are preferred for storage, transport, and export. Thus, soybean breeding should focus on improvement of TW. The objectives of this study were to determine genotypic and environmental effects on TW of soybean and explore relationships of TW with yield, maturity, 100 seed weight (SDWT), seed quality (SQ), seed oil concentration (SOC), and seed protein concentration (SPC). Three sets of breeding populations (BPs), two mapping populations (MPs), and five different high vs. low seed protein near‐isogenic lines populations (NILPs) were used. The BPs and MPs had an average range of 3.5 kg hectoliter (hL)–1 in TW among genotypes. The average ranges of TW in the NILPs were equal for both low protein (LP) and high protein (HP) lines (2.7 kg hL–1). Broad‐sense heritability estimates for TW ranged from .62 to .95. The genotypic and G × E effects on TW were significant in all trials. The SDWT, SQ, SOC, and SPC had strong significant relationships with TW in multiple trials, but were inconsistent overall. Test weight of NILPs differing by presence of ‘Danbaekkong’ high protein allele on chromosome 20 had mostly significant positive and negative relationships with SPC and SOC,  respectively.}, journal={AGRONOMY JOURNAL}, author={McNeece, Brant T. and Gillenwater, Jay H. and Li, Zenglu and Mian, M. A. Rouf}, year={2021}, month={May} }
 @article{gillenwater_mcneece_taliercio_mian_2021, title={QTL mapping of seed protein and oil traits in two recombinant inbred line soybean populations}, ISSN={["1542-7536"]}, DOI={10.1080/15427528.2021.1985028}, abstractNote={ABSTRACT Seed oil and seed protein contents are commercially important components of soybean (Glycine max (L.) Merr.) that are inversely correlated. The objectives of this study were to identify novel quantitative trait loci (QTL) and validate existing QTL associated with seed oil, seed protein, and seed weight in soybean. Two mapping populations, Pop 201 and Pop 202, consisting of 180 and 170 recombinant inbred lines (RILs), respectively, were used in this study. The phenotypic data for each population were collected from four environments. The linkage maps of Pop 201 and Pop 202 consisted of 421 and 416 polymorphic single nucleotide polymorphism (SNP) markers, respectively. Multiple QTL Mapping (MQM) analyses identified a total of 13 QTL for seed oil, 7 QTL for seed protein, and 6 for seed weight (SDWT). QTL for seed oil content not co-located with protein QTL were found on chromosomes 17 and 18 in multiple environments in Pop 201 and Pop 202, respectively. These QTL can be useful in reducing the inverse correlation between seed protein and seed oil contents. Most QTL found in this study are in previously reported genomic regions, and thus provide additional evidence for the stability of those QTL across genetic and environmental backgrounds. The findings of this study provide additional insight into the genetic control of these traits and potentially enable breeders to utilize the QTL-linked SNPs in marker-assisted selection (MAS).}, journal={JOURNAL OF CROP IMPROVEMENT}, author={Gillenwater, Jay H. and McNeece, Brant T. and Taliercio, Earl and Mian, M. A. Rouf}, year={2021}, month={Oct} }
 @article{mian_mcneece_gillen_carter_bagherzadi_2021, title={Registration of USDA-N6005 germplasm combining high yield, elevated protein, and 25% pedigree from Japanese cultivar Tamahikari}, volume={15}, ISSN={["1940-3496"]}, DOI={10.1002/plr2.20139}, abstractNote={AbstractUSDA‐N6005 soybean [Glycine max (L.) Merr.] (Reg. no. GP‐442, PI 696388), is an F5–derived early maturity group (MG) VI germplasm jointly released by the USDA–ARS and North Carolina Agricultural Research Service in January 2021. USDA‐N6005 is a genetically diverse germplasm with 25% of its pedigree from Japanese cultivar Tamahikari and has high yield potential coupled with elevated seed and meal protein content. USDA‐N6005 is the first MG VI release derived from Tamahikari. In the USDA Uniform Tests–Southern States during 2017–2018, USDA‐N6005 yielded 102, 103, and 102% of the check cultivars NC‐Dunphy, NC‐Dilday, and NC‐Roy, respectively. It had significantly better lodging resistance (1.5) than NC‐Roy and NC‐Dilday (2.6 and 2.5, respectively). The seed protein content on a dry basis of USDA‐N6005 (424 g kg–1) was significantly higher than that of NC‐Dunphy and NC‐Dilday (392 and 383 g kg–1, respectively). The estimated meal protein content (49.0%) of USDA‐N6005 was significantly higher than that of NC‐Dunphy and NC‐Dilday (46.2 and 45.5%, respectively). Across the five environments of the 2016 USDA Preliminary Tests–Southern States, USDA‐N6005 yielded 100 and 102% of check cultivars AG6534 and NC‐Roy, respectively. This release should help to reverse the declining trend in genetic diversity and seed protein of U.S. soybean cultivars without negative impact on seed yield.}, number={2}, journal={JOURNAL OF PLANT REGISTRATIONS}, author={Mian, M. A. Rouf and McNeece, Brant T. and Gillen, Anne M. and Carter, Thomas E., Jr. and Bagherzadi, Laleh}, year={2021}, month={May}, pages={388–394} }
 @article{mcneece_bagherzadi_carter_mian_2020, title={Registration of USDA-N7004 soybean germplasm with good yield, elevated seed protein, and 25% exotic pedigree from Tamahikari}, volume={14}, ISSN={["1940-3496"]}, DOI={10.1002/plr2.20039}, abstractNote={AbstractUSDA‐N7004 (Reg. no. GP‐438, PI 692263) is a conventional late maturity group VII soybean [Glycine max (L.) Merr.] germplasm with good yield potential, elevated seed protein content, and 25% exotic pedigree from Japanese cultivar Tamahikari (PI 423897). USDA‐N7004 was jointly released as a non‐GM germplasm by the USDA‐ARS and the North Carolina Agricultural Research Service in September 2019. USDA‐N7004, experimental name N10‐711, was derived from a cross of USDA‐ARS breeding lines NTCPR01‐163 (‘Dillon’ × Tamahikari) and N03‐832. Across 27 environments of the USDA Uniform Soybean Tests‐Southern States (Uniform Tests), USDA‐N7004 yielded 97 and 94% of elite checks ‘N7003CN’ and ‘NC‐Wilder’, respectively. Over 20 environments of United Soybean Board Southern Diversity Yield Trials (Diversity Trials), USDA‐N7004 yielded 98 and 94% of the same two checks, respectively. In the Uniform Tests, seed protein content of USDA‐N7004 (421 g kg−1) was significantly higher than N7003CN and NC‐Wilder on a zero‐moisture basis (405 and 399 g kg−1, respectively). The meal protein content of USDA‐N7004 (49%) was significantly higher than N7003CN or NC‐Wilder (47%) in the Uniform Tests as well as in the Diversity Trials. USDA‐N7004 should be a useful parent in breeding programs aimed at broadening the genetic base of soybean along with improving seed protein and seed yield.}, number={3}, journal={JOURNAL OF PLANT REGISTRATIONS}, author={McNeece, Brant T. and Bagherzadi, Laleh and Carter, Thomas E., Jr. and Mian, M. A. Rouf}, year={2020}, month={Sep}, pages={431–436} }
 @article{xavier_jarquin_howard_ramasubramanian_specht_graef_beavis_diers_song_cregan_et al._2018, title={Genome-Wide Analysis of Grain Yield Stability and Environmental Interactions in a Multiparental Soybean Population}, volume={8}, ISSN={["2160-1836"]}, DOI={10.1534/g3.117.300300}, abstractNote={AbstractGenetic improvement toward optimized and stable agronomic performance of soybean genotypes is desirable for food security. Understanding how genotypes perform in different environmental conditions helps breeders develop sustainable cultivars adapted to target regions. Complex traits of importance are known to be controlled by a large number of genomic regions with small effects whose magnitude and direction are modulated by environmental factors. Knowledge of the constraints and undesirable effects resulting from genotype by environmental interactions is a key objective in improving selection procedures in soybean breeding programs. In this study, the genetic basis of soybean grain yield responsiveness to environmental factors was examined in a large soybean nested association population. For this, a genome-wide association to performance stability estimates generated from a Finlay-Wilkinson analysis and the inclusion of the interaction between marker genotypes and environmental factors was implemented. Genomic footprints were investigated by analysis and meta-analysis using a recently published multiparent model. Results indicated that specific soybean genomic regions were associated with stability, and that multiplicative interactions were present between environments and genetic background. Seven genomic regions in six chromosomes were identified as being associated with genotype-by-environment interactions. This study provides insight into genomic assisted breeding aimed at achieving a more stable agronomic performance of soybean, and documented opportunities to exploit genomic regions that were specifically associated with interactions involving environments and subpopulations.}, number={2}, journal={G3-GENES GENOMES GENETICS}, author={Xavier, Alencar and Jarquin, Diego and Howard, Reka and Ramasubramanian, Vishnu and Specht, James E. and Graef, George L. and Beavis, William D. and Diers, Brian W. and Song, Qijian and Cregan, Perry B. and et al.}, year={2018}, month={Feb}, pages={519–529} }
 @article{la mantia_mian_redinbaugh_2018, title={Identification of Soybean Host Plant Resistance to Brown Marmorated Stink Bugs in Maturity Group III Plant Introductions}, volume={111}, ISSN={["1938-291X"]}, DOI={10.1093/jee/tox295}, abstractNote={Abstract Halyomorpha halys (Stål; Hemiptera: Pentatomidae), brown marmorated stink bug (BMSB), is a polyphagous nonnative insect first found in the United States in 1996. As of 2017, BMSB has been detected in 43 states and is a severe agricultural pest in mid-Atlantic states. On soybean, Glycine max (L.) Merr (Fabales: Fabaceae), damage from BMSB infestation ranges from puncture marks with seed discoloration and deformities to seed and pod abortion. Host plant resistance has been used for managing pest populations and mitigating soybean yield losses caused by neotropical stink bugs (Eushistus heros, Nezara viridula, and Piezodorus guildinii) in Brazil and on the U.S. Gulf Coast. We evaluated maturity group III plant introductions (PIs) for resistance to BMSB damage. In 2014, field cage choice tests of 106 PIs revealed a range of both BMSB damage incidence and severity. In field choice tests, PIs 085665 and 097139 showed the lowest incidence of BMSB damage and seed weight loss due to BMSB, while PIs 243532, 243540, and 567252 had the highest. In whole plant no-choice tests, PIs 085665 and 097139 also had high levels of resistance. However, PI 085665 had a higher incidence of damage but lower seed weight loss than PI 097139, which may suggest bimodal resistance. Moreover, PIs 085665 and 097139 are from Japan and North Korea, respectively, two geographically isolated countries where BMSB is native.Thus, further characterization of host plant resistance to BMSB in each of these lines may elucidate distinct mechanisms that could be synergistic if stacked in breeding lines.}, number={1}, journal={JOURNAL OF ECONOMIC ENTOMOLOGY}, author={La Mantia, Jonathan M. and Mian, M. A. Rouf and Redinbaugh, Margaret G.}, year={2018}, month={Feb}, pages={428–434} }