Roma N. Agrawal

Works (1)

Updated: July 5th, 2023 15:39

2016 journal article

Identifying and Visualizing Functional PAM Diversity across CRISPR-Cas Systems

MOLECULAR CELL, 62(1), 137–147.

By: R. Leenay n, K. Maksimchuk n, R. Slotkowski n, R. Agrawal n, A. Gomaa n, A. Briner n, R. Barrangou n , C. Beisel n

MeSH headings : Bacillus / chemistry; Bacillus / metabolism; CRISPR-Associated Proteins / chemistry; CRISPR-Associated Proteins / genetics; CRISPR-Cas Systems; Escherichia coli / chemistry; Escherichia coli / metabolism; Francisella / chemistry; Francisella / metabolism; High-Throughput Screening Assays / methods; Protein Structure, Tertiary; Streptococcus thermophilus / chemistry; Streptococcus thermophilus / metabolism
TL;DR: An in vivo, positive, and tunable screen termed PAM-SCANR (PAM screen achieved by NOT-gate repression) to elucidate functional PAMs as well as an interactive visualization scheme termed the PAM wheel to convey individual PAM sequences and their activities to accelerate ability to understand and exploit the multitude of CRISPR-Cas systems in nature. (via Semantic Scholar)
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Sources: Web Of Science, ORCID, NC State University Libraries
Added: August 6, 2018

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