Rajesh Paul

College of Engineering

Works (10)

Updated: April 23rd, 2025 23:36

2025 journal article

Non‐destructive seed genotyping via microneedle‐based DNA extraction

Plant Biotechnology Journal, 23(6), 2317–2329.

By: M. Li n, A. Poonam n, Q. Cui  n, T. Hsieh n , S. Jagadeesan n, J. Xu n, W. Bruce*, J. Vogel * ...

author keywords: seed genotyping; DNA extraction; microneedle; breeding; sequencing
topics (OpenAlex): Plant tissue culture and regeneration; Plant Virus Research Studies; Soybean genetics and cultivation
UN Sustainable Development Goals Color Wheel
UN Sustainable Development Goal Categories
2. Zero Hunger (OpenAlex)
13. Climate Action (Web of Science)
15. Life on Land (Web of Science)
Sources: Web Of Science, ORCID, NC State University Libraries, Crossref
Added: March 24, 2025

2024 journal article

Rapid Detection of Viral, Bacterial, Fungal, and Oomycete Pathogens on Tomatoes with Microneedles, LAMP on a Microfluidic Chip, and Smartphone Device

Phytopathology®, 114(8), 1975–1983.

By: T. Shymanovich n, A. Saville n , R. Paul n, Q. Wei n  & J. Ristaino n 

author keywords: bacterial pathogens; fungal pathogens; oomycetes; pathogen detection
MeSH headings : Solanum lycopersicum / virology; Solanum lycopersicum / microbiology; Plant Diseases / virology; Plant Diseases / microbiology; Nucleic Acid Amplification Techniques / instrumentation; Nucleic Acid Amplification Techniques / methods; Smartphone; Needles / virology; Needles / microbiology; Oomycetes / isolation & purification; Lab-On-A-Chip Devices; Fungi / isolation & purification; Molecular Diagnostic Techniques
topics (OpenAlex): Plant Pathogens and Fungal Diseases; Advanced Biosensing Techniques and Applications; Allergic Rhinitis and Sensitization
Sources: Web Of Science, NC State University Libraries, ORCID, Crossref
Added: August 19, 2024

2023 journal article

Abaxial leaf surface-mounted multimodal wearable sensor for continuous plant physiology monitoring

Science Advances, 9(15).

By: G. Lee n, O. Hossain n, S. Jamalzadegan n, Y. Liu n, H. Wang n, A. Saville n , T. Shymanovich n, R. Paul n ...

MeSH headings : Wearable Electronic Devices; Plant Leaves; Temperature; Plant Physiological Phenomena; Volatile Organic Compounds; Plants
topics (OpenAlex): Advanced Chemical Sensor Technologies; Analytical Chemistry and Sensors; Gas Sensing Nanomaterials and Sensors
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Sources: Web Of Science, NC State University Libraries, ORCID, Crossref
Added: June 5, 2023

2023 article

Antiviral Peptides in Antimicrobial Surface Coatings—From Current Techniques to Potential Applications

Jabeen, M., Biswas, P., Islam, M. T., & Paul, R. (2023, February 27). Viruses.

By: M. Jabeen n, P. Biswas *, M. Islam * & R. Paul n

author keywords: peptides; antimicrobial coatings; surface coatings; antimicrobial peptides; antiviral peptides
MeSH headings : Humans; Antiviral Agents / pharmacology; COVID-19 / prevention & control; Anti-Infective Agents / pharmacology; Anti-Bacterial Agents / chemistry; Peptides / pharmacology
topics (OpenAlex): Antimicrobial Peptides and Activities; Antimicrobial agents and applications; Polydiacetylene-based materials and applications
TL;DR: A review on potential techniques to incorporate peptides in current surface coating strategies that will serve as a guide for developing cost-effective, sustainable and coherent antiviral surface coatings is presented. (via Semantic Scholar)
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Source: Web Of Science
Added: April 17, 2023

2021 journal article

Integrated microneedle-smartphone nucleic acid amplification platform for in-field diagnosis of plant diseases

Biosensors and Bioelectronics, 187, 113312.

By: R. Paul n, E. Ostermann n, Y. Chen n, A. Saville n , Y. Yang *, Z. Gu*, A. Whitfield n , J. Ristaino n , Q. Wei n 

author keywords: Plant disease diagnostics; Nucleic acid extraction; Microneedle; Isothermal amplification; Smartphone
MeSH headings : Biosensing Techniques; Molecular Diagnostic Techniques; Nucleic Acid Amplification Techniques; Plant Diseases; Smartphone
topics (OpenAlex): Biosensors and Analytical Detection; Plant Virus Research Studies; Advanced Biosensing Techniques and Applications
TL;DR: It is found that the MN patch containing as few as a single needle tip successfully extracted enough RNA for RT-PCR or RT-LAMP analysis, and MN-extracted RNA samples remained stable on MN surfaces for up to three days. (via Semantic Scholar)
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Sources: Web Of Science, NC State University Libraries, ORCID, Crossref
Added: June 14, 2021

2021 article

Real-time monitoring of plant stresses via chemiresistive profiling of leaf volatiles by a wearable sensor

Li, Z., Liu, Y., Hossain, O., Paul, R., Yao, S., Wu, S., … Wei, Q. (2021, July 1). Matter, Vol. 7, pp. 2553–2570.

By: Z. Li  n, Y. Liu n, O. Hossain n, R. Paul n, S. Yao n, S. Wu n , J. Ristaino n , Y. Zhu n , Q. Wei n 

Contributors: Z. Li  n, Y. Liu n, O. Hossain n, R. Paul n, S. Yao n, S. Wu n , J. Ristaino n , Y. Zhu n , Q. Wei n 

topics (OpenAlex): Advanced Chemical Sensor Technologies; Analytical Chemistry and Sensors; Gas Sensing Nanomaterials and Sensors
TL;DR: A leaf-attachable chemiresistive sensor array for real-time fingerprinting of volatile organic compounds (VOCs) that permits noninvasive and early diagnosis of plant diseases, such as late blight caused by Phytophthora infestans is reported. (via Semantic Scholar)
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16. Peace, Justice and Strong Institutions (OpenAlex)
Sources: Web Of Science, NC State University Libraries, ORCID
Added: July 26, 2021

2020 article

Advances in point-of-care nucleic acid extraction technologies for rapid diagnosis of human and plant diseases

Paul, R., Ostermann, E., & Wei, Q. (2020, September 7). Biosensors and Bioelectronics, Vol. 169.

By: R. Paul n, E. Ostermann n & Q. Wei n 

author keywords: Point-of-care diagnostics; DNA/RNA extraction; Nucleic acid amplification; Raw samples; Infectious diseases; Plant diseases
MeSH headings : Betacoronavirus / isolation & purification; COVID-19; Chemical Fractionation / instrumentation; Chemical Fractionation / methods; Communicable Diseases / diagnosis; Communicable Diseases / microbiology; Communicable Diseases / parasitology; Communicable Diseases / virology; Coronavirus Infections / diagnosis; Coronavirus Infections / virology; Equipment Design; Humans; Lab-On-A-Chip Devices; Nucleic Acid Amplification Techniques / instrumentation; Nucleic Acid Amplification Techniques / methods; Nucleic Acids / blood; Nucleic Acids / isolation & purification; Nucleic Acids / urine; Pandemics; Plant Diseases / microbiology; Plant Diseases / parasitology; Plant Diseases / virology; Pneumonia, Viral / diagnosis; Pneumonia, Viral / virology; Point-of-Care Systems; SARS-CoV-2
topics (OpenAlex): Biosensors and Analytical Detection; Bacteriophages and microbial interactions; SARS-CoV-2 detection and testing
TL;DR: This review focuses on novel devices or methods that have demonstrated applicability and robustness for the isolation of high-quality nucleic acid from complex raw samples, such as human blood, saliva, sputum, nasal swabs, urine, and plant tissues that would pave the road for the “sample-in-result-out” diagnosis of human and plant diseases, especially in remote or resource-limited settings. (via Semantic Scholar)
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Sources: Web Of Science, NC State University Libraries
Added: November 16, 2020

2020 article

Agricultural nanodiagnostics for plant diseases: recent advances and challenges

Li, Z., Yu, T., Paul, R., Fan, J., Yang, Y., & Wei, Q. (2020, January 1). Nanoscale Advances, Vol. 2, pp. 3083–3094.

By: Z. Li n, T. Yu n, R. Paul n, J. Fan *, Y. Yang * & Q. Wei n 

topics (OpenAlex): Advanced Chemical Sensor Technologies; Biosensors and Analytical Detection; Gas Sensing Nanomaterials and Sensors
UN Sustainable Development Goals Color Wheel
UN Sustainable Development Goal Categories
2. Zero Hunger (OpenAlex)
Sources: Web Of Science, NC State University Libraries, ORCID
Added: August 31, 2020

2019 journal article

Detection of Phytophthora infestans by Loop-Mediated Isothermal Amplification, Real-Time LAMP, and Droplet Digital PCR

Plant Disease, 104(3), 708–716.

By: J. Ristaino n , A. Saville n , R. Paul n, D. Cooper* & Q. Wei n 

author keywords: oomycetes; pathogen detection; Phytophthora infestans; vegetables
MeSH headings : Solanum lycopersicum; Nucleic Acid Amplification Techniques; Phytophthora infestans; Polymerase Chain Reaction; Solanum tuberosum
topics (OpenAlex): Plant Pathogens and Resistance; Biosensors and Analytical Detection; Plant Pathogens and Fungal Diseases
TL;DR: The loop-mediated isothermal amplification (LAMP) assay was specific for P. infestans on potato and tomato and did not amplify other potato- or tomato-infecting Phytophthora species or other fungal and bacterial pathogens that infect potato and Tomato. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries, Crossref
Added: April 6, 2020

2019 journal article

Extraction of Plant DNA by Microneedle Patch for Rapid Detection of Plant Diseases

ACS Nano, 13(6), 6540–6549.

By: R. Paul n, A. Saville n , J. Hansel n, Y. Ye n, C. Ball n, A. Williams  n, X. Chang*, G. Chen n ...

author keywords: plant disease; DNA extraction; microneedle patch; Phytophthora infestans; nucleic acid amplification; point-of-care diagnostics
MeSH headings : DNA Barcoding, Taxonomic / instrumentation; DNA Barcoding, Taxonomic / methods; DNA, Fungal / chemistry; DNA, Fungal / genetics; Solanum lycopersicum / microbiology; Metagenomics / instrumentation; Metagenomics / methods; Needles; Phytophthora / genetics; Phytophthora / pathogenicity; Plant Diseases / microbiology; Plant Leaves / chemistry; Plant Leaves / microbiology
topics (OpenAlex): Transgenic Plants and Applications; Plant tissue culture and regeneration; Plant Virus Research Studies
TL;DR: A simple, cell-lysis-free, and purification-free DNA extraction method that could be a transformative approach to facilitate rapid sample preparation for molecular diagnosis of various plant diseases directly in the field is developed. (via Semantic Scholar)
UN Sustainable Development Goals Color Wheel
UN Sustainable Development Goal Categories
Sources: Web Of Science, NC State University Libraries, Crossref
Added: July 15, 2019

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