Ravi Kulkarni

Also known as: Ravi Kulkarni

Immunology, Infectious diseases, Vaccines, poultry, gut health

Works (60)

Updated: June 1st, 2024 05:15

2024 journal article

Characterization of immune responses and immunopathology in turkeys experimentally infected with clostridial dermatitis-producing strains of Clostridium septicum

VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY, 269.

By: V. Criollo n, F. John n, C. Gaghan n, O. Fletcher n, A. Thachil*, R. Crespo n, R. Kulkarni n

author keywords: Clostridial dermatitis; Turkey; Clostridium septicum; Immune response; Immunopathology
Sources: Web Of Science, NC State University Libraries
Added: April 8, 2024

2024 article

Evaluation of Adjuvant Effect of Cytosine-Guanosine-Oligodeoxynucleotide in Meat-Type Chickens Coadministered <i>In Ovo</i> with Herpesvirus of Turkey Vaccine

Boone, A. C., Kulkarni, R. R., Cortes, A. L., Gaghan, C., Mohammed, J., Villalobos, T., … Gimeno, I. M. (2024, February 1). VIRAL IMMUNOLOGY, Vol. 2.

By: A. Boone n, R. Kulkarni n, A. Cortes n, C. Gaghan n, J. Mohammed*, T. Villalobos*, J. Esandi*, I. Gimeno n

author keywords: herpesvirus of turkey; toll-like receptor 21; cellular immunity; immunocompetence; in ovo vaccination; chickens
TL;DR: Adding cytosine-guanosine oligodeoxynucleotides (CpG ODNs) to the HVT vaccine could enhance the adjuvant effect of HVT, and CpG ODN administration in ovo with or without HVT significantly increased frequencies of activated CD4+ and CD8+ T cells. (via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being (OpenAlex)
Sources: Web Of Science, NC State University Libraries, ORCID
Added: February 2, 2024

2024 journal article

Immunization of turkeys with <i>Clostridium septicum</i> alpha toxin-based recombinant subunit proteins can confer protection against experimental Clostridial dermatitis

PLOS ONE, 19(4).

By: F. John n, V. Criollo n, C. Gaghan n, A. Armwood n, J. Holmes n, A. Thachil*, R. Crespo n, R. Kulkarni n

Ed(s): Y. Chang

UN Sustainable Development Goal Categories
3. Good Health and Well-being (OpenAlex)
Sources: ORCID, Web Of Science, NC State University Libraries
Added: May 1, 2024

2024 journal article

In ovo feeding of probiotic lactobacilli differentially alters expression of genes involved in the development and immunological maturation of bursa of Fabricius in pre-hatched chicks

POULTRY SCIENCE, 103(1).

author keywords: chicken; probiotics; Lactobacillus; cytokine; bursa of Fabricius
Sources: Web Of Science, NC State University Libraries, ORCID
Added: January 2, 2024

2024 article

Modulation of functional activity of heat-stressed chicken macrophages by poultry-derived probiotic lactobacilli

Abdelaziz, K., Nixon, T., Joye, A., Hassan, H., Alizadeh, M., Sharif, S., & Kulkarni, R. R. (2024, April 9). CANADIAN JOURNAL OF ANIMAL SCIENCE, Vol. 4.

author keywords: chicken; Lactobacillus; macrophage; cytokine; TLR; heat stress
Sources: Web Of Science, NC State University Libraries
Added: April 15, 2024

2023 journal article

Cellular Immune Responses in Lymphoid Tissues of Broiler Chickens Experimentally Infected with Necrotic Enteritis–Producing Clostridium perfringens Strains

Avian Diseases, 67(2).

MeSH headings : Animals; Clostridium perfringens / physiology; Clostridium Infections / veterinary; Clostridium Infections / pathology; Chickens; CD8-Positive T-Lymphocytes / pathology; Enteritis / veterinary; Enteritis / pathology; Poultry Diseases / pathology; Lymphoid Tissue / pathology; Immunity, Cellular; Necrosis / veterinary
Source: ORCID
Added: August 19, 2023

2023 journal article

Effect of CpG-Oligonucleotide in Enhancing Recombinant Herpes Virus of Turkey-Laryngotracheitis Vaccine-Induced Immune Responses in One-Day-Old Broiler Chickens

VACCINES, 11(2).

author keywords: infectious laryngotracheitis; CpG-ODN; adjuvant; immune response; chickens
TL;DR: Findings suggested that CpG-ODN can boost rHVT-LT-induced immune responses in day-old chicks, which may help in anti-ILT defense during their later stages of life. (via Semantic Scholar)
Sources: ORCID, Web Of Science, NC State University Libraries
Added: January 31, 2023

2023 article

Effects of <i>in ovo</i> inoculation of multi-strain lactobacilli on cytokine gene expression and antibody-mediated immune responses in chickens (vol 10, 1252518, 2023)

Alizadeh, M., Shojadoost, B., Astill, J., Taha-Abdelaziz, K., Karimi, S. H., Bavananthasivam, J., … Sharif, S. (2023, October 9). FRONTIERS IN VETERINARY SCIENCE, Vol. 10.

By: M. Alizadeh*, B. Shojadoost*, J. Astill*, K. Taha-Abdelaziz*, S. Karimi*, J. Bavananthasivam*, R. Kulkarni n, S. Sharif*

author keywords: lactobacilli; in ovo; chickens; cytokines; antibody
Sources: Web Of Science, NC State University Libraries
Added: November 13, 2023

2023 journal article

Immune Response Evaluation in Commercial Turkeys Affected with Clostridial Dermatitis

AVIAN DISEASES, 67(1), 80–88.

By: V. Criollo n, C. Gaghan n, F. John n, E. Orozco, A. Thachil*, R. Crespo n, R. Kulkarni n

author keywords: clostridial dermatitis; gangrenous dermatitis; Clostridium septicum; immune response; macrophages
MeSH headings : Animals; Clostridium Infections / veterinary; Turkeys; Interleukin-13; Poultry Diseases / epidemiology; Clostridium; Dermatitis / veterinary; Immunity
UN Sustainable Development Goal Categories
3. Good Health and Well-being (Web of Science; OpenAlex)
Sources: Web Of Science, NC State University Libraries
Added: May 22, 2023

2023 journal article

In Ovo Vaccination with Recombinant Herpes Virus of the Turkey-Laryngotracheitis Vaccine Adjuvanted with CpG-Oligonucleotide Provides Protection against a Viral Challenge in Broiler Chickens

VIRUSES-BASEL, 15(10).

By: C. Gaghan n, M. Browning n, A. Fares n, M. Abdul-Careem*, I. Gimeno n & R. Kulkarni n

author keywords: infectious laryngotracheitis; vaccine; protection; CpG oligonucleotide; adjuvant; chickens
TL;DR: It is suggested that CpG-ODN can be used as an effective adjuvant for rHVT-LT in ovo vaccination to induce protective immunity against ILT in broiler chickens. (via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being (OpenAlex)
Sources: Web Of Science, NC State University Libraries, ORCID
Added: October 18, 2023

2023 journal article

In ovo HVT vaccination enhances cellular responses at hatch and addition of poly I:C offers minimal adjuvant effects

VACCINE, 41(15), 2514–2523.

By: A. Boone n, R. Kulkarni n, A. Cortes n, T. Villalobos*, J. Esandi* & I. Gimeno n

author keywords: Herpesvirus of turkey; Toll -like receptor 3; Innate and Cellular immunity; Immunocompetence; In ovo vaccination; Chickens
MeSH headings : Animals; Chickens; Poly I-C / pharmacology; Toll-Like Receptor 3; Interleukin-13; CD28 Antigens; Marek Disease; Herpesvirus 1, Meleagrid; Interferon-gamma; Vaccination / veterinary
TL;DR: The cellular mechanisms by which HVT-RD can hasten immunocompetence in one-day-old meat-type chickens are evaluated, and it is determined if HVT adjuvantation with a TLR3 agonist, polyinosinic-polycytidylic acid (poly(I:C)), could enhance vaccine-induced responses and provide dose-sparing effects. (via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being (Web of Science; OpenAlex)
Sources: ORCID, Web Of Science, NC State University Libraries
Added: March 8, 2023

2023 review

Intervention Strategies to Control Campylobacter at Different Stages of the Food Chain

[Review of ]. MICROORGANISMS, 11(1).

By: K. Taha-Abdelaziz*, M. Singh*, S. Sharif*, S. Sharma*, R. Kulkarni n, M. Alizadeh*, A. Yitbarek*, Y. Helmy*

author keywords: chicken; Campylobacter; vaccine; feed additives; probiotics; prebiotics; synbiotics; bacteriophages; bacteriocins; organic acids; essential oils; small molecules; short-chain fatty acids
Sources: ORCID, Web Of Science, NC State University Libraries
Added: January 2, 2023

2023 journal article

Intracloacal Inoculation of Broiler Chickens with Clostridium perfringens Strains: Evaluation of Necrotic Enteritis Disease Development and Lymphoid Immune Responses

MICROORGANISMS, 11(3).

By: C. Gaghan n, K. Gorrell n, K. Taha-Abdelaziz*, S. Sharif* & R. Kulkarni n

author keywords: necrotic enteritis; Clostridium perfringens; chickens; intracloacal infection; immune response; virulence
TL;DR: Intracloacal C. perfringens inoculation seems to induce a highly regulated inflammatory response in the CT and other mucosal lymphoid organs and an intracloACal infection model may be useful in evaluating immune responses in chickens with subclinical NE. (via Semantic Scholar)
Sources: ORCID, Web Of Science, NC State University Libraries
Added: March 18, 2023

2023 article

Mucosal and systemic lymphoid immune responses against Clostridium perfringens strains with variable virulence in the production of necrotic enteritis in broiler chickens

Kulkarni, R. R., Gaghan, C., Gorrell, K., & Fletcher, O. J. (2023, January 26). AVIAN PATHOLOGY, Vol. 1.

author keywords: Necrotic enteritis; Clostridium perfringens; chickens; immune response; virulence; cytokines; pathogenesis
MeSH headings : Animals; Clostridium perfringens / genetics; Clostridium Infections / veterinary; Chickens; Virulence; Enteritis / veterinary; Poultry Diseases / pathology; Immunity; Anti-Inflammatory Agents / metabolism
TL;DR: It is suggested that lymphoid immune responses during NE in chickens are spatially regulated such that the inflammatory responses against C. perfringens depend on the virulence of the strain. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Sources: Web Of Science, NC State University Libraries
Added: March 13, 2023

2022 journal article

A flow cytometric method for enumeration and speciation of coccidia affecting broiler chickens

VETERINARY PARASITOLOGY, 301.

Contributors: D. Adams n, R. Kulkarni n, J. Mohammed n & R. Crespo n

author keywords: Flow cytometry; Eimeria; Coccidiosis; Oocyst; Quantification
MeSH headings : Animals; Chickens; Coccidiosis / veterinary; Eimeria / genetics; Flow Cytometry / veterinary; Poultry Diseases; Real-Time Polymerase Chain Reaction / veterinary
TL;DR: It is suggested that non-antibody based Flow Cytometry technique can be successful in the simultaneous enumeration and speciation of coccidia and other Eimeria species. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Sources: ORCID, Web Of Science, NC State University Libraries
Added: December 1, 2021

2022 journal article

AAV-monoclonal antibody expression protects mice from Ebola virus without impeding the endogenous antibody response to heterologous challenge

MOLECULAR THERAPY-NUCLEIC ACIDS, 26, 505–518.

By: L. Lieshout*, A. Rghei*, W. Cao*, S. He*, G. Soule*, W. Zhu*, S. Thomas*, D. Sorensen* ...

TL;DR: It is demonstrated that AAV6.2FF-mediated expression of murine IgG2a EBOV mAbs, 2G4 and 5D2, protects from mouse-adapted (MA)-EBOV infection with none of the surviving mice developing anti-VP40 antibodies above background. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: October 24, 2022

2022 journal article

Avian Macrophage Responses to Virulent and Avirulent Clostridium perfringens

PATHOGENS, 11(1).

author keywords: macrophages; Clostridium perfringens; virulence; chicken; necrotic enteritis; immune response
TL;DR: Macrophage interaction with C. perfringens can lead to cellular activation and, the ability of this pathogen to induce macrophage responses may depend on its level of virulence (via Semantic Scholar)
Sources: ORCID, Web Of Science, NC State University Libraries
Added: January 17, 2022

2022 journal article

Characterization of vaccine-induced immune responses against coccidiosis in broiler chickens

VACCINE, 40(29), 3893–3902.

author keywords: Coccidiosis; Eimeria; Vaccine; Immunity; Immune response
MeSH headings : Animals; Chickens; Coccidiosis / prevention & control; Coccidiosis / veterinary; Eimeria; Eimeria tenella; Immunity; Oocysts; Poultry Diseases; Protozoan Vaccines; Toll-Like Receptor 2
TL;DR: Findings suggest that TLR21 and TLR2 may be involved in the immune cell recognition of Eimeria parasites and that the vaccine can induce a robust macrophage activation leading to a T helper-1 dominated protective response at both local and systemic lymphoid tissues. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Sources: ORCID, Web Of Science, NC State University Libraries
Added: May 27, 2022

2022 article

Effects of Treatment with Lactobacilli on Necrotic Enteritis in Broiler Chickens

Shojadoost, B., Alizadeh, M., Boodhoo, N., Astill, J., Karimi, S. H., Doost, J. S., … Sharif, S. (2022, January 19). PROBIOTICS AND ANTIMICROBIAL PROTEINS, Vol. 1.

By: B. Shojadoost*, M. Alizadeh*, N. Boodhoo*, J. Astill*, S. Karimi*, J. Doost*, K. Taha-Abdelaziz*, R. Kulkarni n, S. Sharif*

author keywords: Necrotic enteritis; Lactobacilli; Immune response; Microbiota; Cytokine expression
MeSH headings : Animals; Male; Chickens; Poultry Diseases / microbiology; Enteritis / therapy; Enteritis / veterinary; Lactobacillus; Clostridium Infections / therapy; Clostridium Infections / veterinary; Clostridium Infections / microbiology; Clostridium perfringens; Anti-Bacterial Agents / pharmacology
TL;DR: It is suggested that the use of certain lactobacilli can reduce NE severity and modulate immune responses and intestinal microbiota composition in chickens. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Sources: Web Of Science, NC State University Libraries
Added: January 31, 2022

2022 journal article

Effects of therapeutic levels of dietary antibiotics on the cecal microbiome composition of broiler chickens

POULTRY SCIENCE, 101(6).

By: S. Kairmi*, K. Taha-Abdelaziz*, A. Yitbarek*, M. Sargolzaei*, H. Spahany*, J. Astill*, B. Shojadoost*, M. Alizadeh* ...

author keywords: microbiome; antibiotics; ionophore; growth promoter; chicken
MeSH headings : Animal Feed / analysis; Animals; Anti-Bacterial Agents / pharmacology; Anti-Bacterial Agents / therapeutic use; Bacteria; Cecum / microbiology; Chickens; Diet / veterinary; Dietary Supplements / analysis; Enteritis / veterinary; Microbiota
UN Sustainable Development Goal Categories
6. Clean Water and Sanitation (OpenAlex)
Sources: Web Of Science, NC State University Libraries
Added: June 6, 2022

2022 journal article

Enumeration and speciation of coccidia affecting turkeys using flow cytometry method

JOURNAL OF APPLIED POULTRY RESEARCH, 31(3).

By: T. Boyett n, R. Crespo n, V. Vinueza n, C. Gaghan n, J. Mohammed n & R. Kulkarni n

author keywords: Eimeria; coccidiosis; turkey; flow cytometry; diagnosis
UN Sustainable Development Goal Categories
15. Life on Land (OpenAlex)
Sources: ORCID, Web Of Science, NC State University Libraries
Added: May 13, 2022

2022 journal article

Ex Vivo Differential Responsiveness to Clostridium perfringens and Lactococcus lactis by Avian Small Intestine Macrophages and T Cells

FRONTIERS IN IMMUNOLOGY, 13.

author keywords: Clostridium perfringens; avian intestinal immunity; Lactococcus lactis; T cell; macrophages; probiotic
MeSH headings : Animals; Chickens; Clostridium perfringens; Intestine, Small; Lactococcus lactis; Macrophages; T-Lymphocyte Subsets
TL;DR: The results demonstrate the potential application of L. lactis, as a probiotic, against virulent C. perfringens infection in chicken and demonstrate varying distribution of innate and adaptive immune cells along the avian gut-associated lymphoid tissue (GALT). (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: March 14, 2022

2022 journal article

Intestinal changes and immune responses during Clostridium perfringens-induced necrotic enteritis in broiler chickens

POULTRY SCIENCE, 101(3).

By: A. Daneshmand*, H. Kermanshahi*, J. Mohammed n, M. Sekhavati*, A. Javadmanesh*, M. Ahmadian*, M. Alizadeh*, J. Razmyar*, R. Kulkarni n

author keywords: Necrotic enteritis; Clostridium perfringens; broiler chicken; histomorphometry; immune response
MeSH headings : Animals; Chickens; Clostridium Infections / pathology; Clostridium Infections / veterinary; Clostridium perfringens / physiology; Diet / veterinary; Enteritis / pathology; Enteritis / veterinary; Escherichia coli; Immunity; Necrosis / veterinary; Poultry Diseases
TL;DR: C. perfringens, while inducing epithelial damage and changes in microbiota, can also trigger host immune responses, and NE reproduction methods using coccidia with or without the wheat-based dietary predisposition seem to facilitate an optimal NE reproduction in broiler chickens. (via Semantic Scholar)
Sources: ORCID, Web Of Science, NC State University Libraries
Added: December 7, 2021

2022 review

Probiotics as Alternatives to Antibiotics for the Prevention and Control of Necrotic Enteritis in Chickens

[Review of ]. PATHOGENS, 11(6).

By: R. Kulkarni n, C. Gaghan n, K. Gorrell n, S. Sharif* & K. Taha-Abdelaziz*

author keywords: necrotic enteritis; Clostridium perfringens; probiotics; disease control; lactobacilli; alternatives to antibiotics
TL;DR: Probiotic species tested in chickens have shown to improve bird performance, reduce C. perfringens colonization and NE-associated pathology, and modulate immune responses by downregulating expression of inflammatory cytokines. (via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger (OpenAlex)
Sources: ORCID, Web Of Science, NC State University Libraries
Added: June 18, 2022

2022 journal article

The Regulatory Microenvironment in Feathers of Chickens Infected with Very Virulent Marek's Disease Virus

VIRUSES-BASEL, 14(1).

author keywords: Marek's disease virus; feather; T regulatory cell; chicken; MD vaccine
MeSH headings : Animals; CD4-Positive T-Lymphocytes; CD8-Positive T-Lymphocytes; Chickens / virology; Feathers / virology; Gene Expression; Herpesvirus 2, Gallid / immunology; Marek Disease / immunology; Marek Disease / virology; Marek Disease Vaccines / immunology; Spleen / immunology; Transforming Growth Factor beta / genetics; Transforming Growth Factor beta / metabolism; Vaccination; Viral Load / veterinary; Virus Replication / physiology
TL;DR: The results of the present study raise the possibility of an immunoregulatory environment in the feather pulp of MDV-infected chickens, which may in turn favor replication of infectious MDV in this tissue. (via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being (Web of Science; OpenAlex)
Sources: Web Of Science, NC State University Libraries
Added: February 7, 2022

2021 review

IMMUNOLOGY, HEALTH AND DISEASE

[Review of ]. POULTRY SCIENCE, 100(4).

author keywords: vitamin A; vitamin C; vitamin D; vitamin E; chicken immune system
MeSH headings : Animals; Antioxidants; Chickens; Immune System; Vitamin A; Vitamins
TL;DR: Benefits of supplementation of these vitamins to poultry may assist the immune system to combat microbial pathogens while reducing detrimental effects associated with stress and enhancing responses to vaccines. (via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger (OpenAlex)
Sources: ORCID, Web Of Science, NC State University Libraries
Added: March 12, 2021

2021 review

Necrotic enteritis in chickens: a review of pathogenesis, immune responses and prevention, focusing on probiotics and vaccination

[Review of ]. ANIMAL HEALTH RESEARCH REVIEWS, 22(2), 147–162.

By: M. Alizadeh*, B. Shojadoost*, N. Boodhoo*, J. Astill, K. Taha-Abdelaziz*, D. Hodgins*, R. Kulkarni n, S. Sharif*

author keywords: Chickens; Clostridium perfringens; immune response; necrotic enteritis; pathogenesis; probiotics; vaccination
MeSH headings : Animals; Chickens; Clostridium Infections / prevention & control; Clostridium Infections / veterinary; Clostridium perfringens / physiology; Enteritis / prevention & control; Enteritis / veterinary; Immunity; Poultry; Poultry Diseases / prevention & control; Probiotics / therapeutic use; Vaccination / veterinary
TL;DR: The roles of management, nutrition, probiotics, and vaccination in reducing the incidence and severity of NE in poultry flocks are discussed. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Sources: Web Of Science, NC State University Libraries
Added: February 7, 2022

2020 journal article

Effects of in ovo Inoculation of Multi-Strain Lactobacilli on Cytokine Gene Expression and Antibody-Mediated Immune Responses in Chickens

FRONTIERS IN VETERINARY SCIENCE, 7.

By: M. Alizadeh*, B. Shojadoost*, J. Astill*, K. Taha-Abdelaziz*, S. Karimi*, J. Bavananthasivam*, R. Kulkarni n, S. Sharif*

TL;DR: It is demonstrated that in ovo administration of lactobacilli can improve antibody-mediated immune responses and differentially modulate cytokine expression in mucosal and systemic lymphoid tissues of chickens. (via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being (OpenAlex)
Sources: Web Of Science, NC State University Libraries
Added: April 27, 2020

2020 article

Gastrointestinal diseases of poultry: causes and nutritional strategies for prevention and control

IMPROVING GUT HEALTH IN POULTRY, Vol. 73, pp. 205–236.

By: R. Kulkarni, K. Taha-Abdelaziz, B. Shojadoost, J. Astill & S. Sharif

UN Sustainable Development Goal Categories
2. Zero Hunger (OpenAlex)
Sources: Web Of Science, NC State University Libraries
Added: August 2, 2021

2020 journal article

In ovo vaccination with herpesvirus of turkey enhances innate and cellular responses in meat-type chickens: Effect of vaccine dose and strain

VACCINE, 38(31), 4837–4845.

By: A. Boone n, T. Kaser n, A. Cortes n, R. Kulkarni n, B. Abad n, T. Villalobos*, J. Esandi*, F. Perozo*, S. Lemiere*, I. Gimeno n

author keywords: Herpesvirus of turkey; Immunocompetence; In ovo vaccination; Chickens
MeSH headings : Animals; Chick Embryo; Chickens; Herpesvirus 1, Meleagrid; Marek Disease; Meat; Poultry Diseases / prevention & control; Vaccination; Viral Vaccines
TL;DR: It is demonstrated in ovo vaccination with HVT in meat-type chickens can accelerate innate and adaptive immunity and one could optimize such effect by modifying the vaccine dose. (via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being (Web of Science; OpenAlex)
Sources: Web Of Science, NC State University Libraries
Added: July 6, 2020

2019 review

Development of innate immunity in chicken embryos and newly hatched chicks: a disease control perspective

[Review of ]. AVIAN PATHOLOGY, 48(4), 288–310.

By: T. Alkie*, A. Yitbarek*, D. Hodgins*, R. Kulkarni*, K. Taha-Abdelaziz* & S. Sharif*

author keywords: Chicken embryos; newly hatched chickens; innate defences; pathogens; PRRs; ontogeny
MeSH headings : Animals; Chick Embryo / immunology; Chickens / immunology; Immunity, Innate; Poultry Diseases / immunology; Poultry Diseases / prevention & control
TL;DR: Improved understanding of innate immune system ontogeny and functionality in chickens is critical for the implementation of sound and safe interventions to provide long-term protection against pathogens. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Sources: Web Of Science, NC State University Libraries
Added: July 22, 2019

2019 journal article

Dietary selenium supplementation enhances antiviral immunity in chickens challenged with low pathogenic avian influenza virus subtype H9N2

Veterinary Immunology and Immunopathology, 207, 62–68.

By: B. Shojadoost*, R. Kulkarni*, A. Yitbarek*, A. Laursen*, K. Taha-Abdelaziz*, T. Negash Alkie*, N. Barjesteh*, W. Quinteiro-Filho* ...

author keywords: Selenium; Chicken; Interferon; Interferon stimulated gene; Avian influenza
MeSH headings : Animals; Chickens / immunology; Chickens / virology; Dietary Supplements; Dose-Response Relationship, Drug; Influenza A Virus, H9N2 Subtype / immunology; Influenza A Virus, H9N2 Subtype / pathogenicity; Influenza in Birds / immunology; Influenza in Birds / prevention & control; Interferons / metabolism; Pharynx / virology; Real-Time Polymerase Chain Reaction / veterinary; Selenium / administration & dosage; Selenium / pharmacology; Spleen / virology; Virus Shedding / drug effects
TL;DR: It is demonstrated that supplementation of chicken diets with selenium, can enhance antiviral defense and thus, may have a beneficial effect in controlling viral infections in poultry. (via Semantic Scholar)
Sources: Crossref, NC State University Libraries
Added: September 8, 2019

2019 journal article

In vitro assessment of immunomodulatory and anti-Campylobacter activities of probiotic lactobacilli

SCIENTIFIC REPORTS, 9.

By: K. Taha-Abdelaziz*, J. Astill*, R. Kulkarni n, L. Read*, A. Najarian*, J. Farber*, S. Sharif*

MeSH headings : Animals; Antibiosis; Caco-2 Cells; Campylobacter jejuni / pathogenicity; Campylobacter jejuni / physiology; Chickens; Cytokines / genetics; Cytokines / metabolism; Humans; Lactobacillus / pathogenicity; Lactobacillus / physiology; Macrophages / immunology; Macrophages / microbiology; Phagocytosis; Quorum Sensing
TL;DR: It is demonstrated that lactobacilli possess anti-Campylobacter and immunomodulatory activities and vary in their ability to elicit innate responses in chicken macrophages. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: December 16, 2019

2019 journal article

Innate antiviral responses are induced by TLR3 and TLR4 ligands in chicken tracheal epithelial cells: Communication between epithelial cells and macrophages

Virology, 534, 132–142.

By: N. Barjesteh*, K. Taha-Abdelaziz*, R. Kulkarni* & S. Sharif*

author keywords: Avian influenza; Chicken; Trachea; Innate immunity; Epithelial cells
MeSH headings : Animals; Avian Proteins / genetics; Avian Proteins / immunology; Chickens; Epithelial Cells / immunology; Epithelial Cells / virology; Immunity, Innate; Influenza A virus / genetics; Influenza A virus / physiology; Influenza in Birds / genetics; Influenza in Birds / immunology; Influenza in Birds / virology; Interferon Regulatory Factors / genetics; Interferon Regulatory Factors / immunology; Interleukin-1beta / genetics; Interleukin-1beta / immunology; Interleukin-8 / genetics; Interleukin-8 / immunology; Ligands; Macrophages / immunology; Macrophages / virology; Poly I-C / immunology; Poultry Diseases / genetics; Poultry Diseases / immunology; Poultry Diseases / virology; Toll-Like Receptor 3 / genetics; Toll-Like Receptor 3 / immunology; Toll-Like Receptor 4 / genetics; Toll-Like Receptor 4 / immunology; Trachea / cytology; Trachea / immunology; Trachea / virology
TL;DR: Evidence is provided that cTECs mount antiviral responses after stimulation with TLR ligands through IRF7 and NF-κB signaling pathways, leading to activation of other cells, such as macrophages, which provided evidence that the role of these cells in the initiation of innate responses is understood. (via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being (Web of Science; OpenAlex)
Sources: Crossref, NC State University Libraries
Added: September 8, 2019

2019 journal article

Interactions between lactobacilli and chicken macrophages induce antiviral responses against avian influenza virus

Research in Veterinary Science, 125, 441–450.

By: B. Shojadoost*, R. Kulkarni*, J. Brisbin*, W. Quinteiro-Filho*, T. Alkie* & S. Sharif*

author keywords: Probiotics; Lactobacilli; Innate immunity; Macrophages; Avian influenza virus
MeSH headings : Animals; Chickens; Influenza A virus / immunology; Influenza in Birds / immunology; Lactobacillus / physiology; Macrophages / immunology; Macrophages / physiology; Probiotics
TL;DR: The results from this study using an in vitro model showed that certain Lactobacillus species can effectively augment antiviral responses in chicken macrophages. (via Semantic Scholar)
Sources: Crossref, NC State University Libraries
Added: August 22, 2019

2018 journal article

Characterizaton of gamma delta T cells in Marek’s disease virus (Gallid herpesvirus 2) infection of chickens

Virology, 522, 56–64.

By: A. Laursen*, R. Kulkarni*, K. Taha-Abdelaziz*, B. Plattner*, L. Read* & S. Sharif*

author keywords: Marek's disease; Chickens; gamma delta T cells; Immune response; Innate lymphocytes; Cytokines
MeSH headings : Animals; CD8-Positive T-Lymphocytes / immunology; Cecum / pathology; Chickens; Cytokines / biosynthesis; Gene Expression Profiling; Herpesvirus 2, Gallid / immunology; Intraepithelial Lymphocytes / immunology; Marek Disease / immunology; Palatine Tonsil / pathology; Poultry Diseases / immunology; Spleen / pathology; Time Factors
TL;DR: A potential role for γδ T cells in host response to GaHV-2 is suggested and the underlying immunological mechanisms of interactions between this virus and its host are elucidated. (via Semantic Scholar)
Sources: Crossref, NC State University Libraries
Added: September 8, 2019

2018 journal article

Immune Priming and Long-term Persistence of Memory B Cells After Inactivated Poliovirus Vaccine in Macaque Models: Support for at least 2 Doses

Clinical Infectious Diseases, 67(suppl_1), S66–S77.

By: S. Bhaumik*, R. Kulkarni*, W. Weldon*, E. Silveira*, H. Ahmed*, S. Gunisetty*, A. Chandele*, R. Antia* ...

author keywords: polio; IPV; memory; priming; B-cells; neutralizing antibodies
MeSH headings : Animals; Antibodies, Neutralizing / immunology; B-Lymphocytes / immunology; Humans; Immunization Schedule; Macaca mulatta; Models, Animal; Poliomyelitis / immunology; Poliomyelitis / prevention & control; Poliomyelitis / virology; Poliovirus / immunology; Poliovirus Vaccine, Inactivated / administration & dosage; Poliovirus Vaccine, Oral / administration & dosage; Vaccination
TL;DR: Two doses of IPV, regardless of whether fractional or full, are more effective than a single dose for inducing long-lasting memory B cells. (via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being (OpenAlex)
Sources: Crossref, NC State University Libraries
Added: August 22, 2019

2018 journal article

Reduction of Marek's Disease Virus Infection by Toll-Like Receptor Ligands in Chicken Embryo Fibroblast Cells

Viral Immunology, 31(5), 389–396.

author keywords: TLR3 ligands; Marek's disease virus; chicken embryo fibroblast cells
MeSH headings : Animals; Cells, Cultured; Chick Embryo; Fibroblasts / virology; Gene Expression Profiling; Herpesvirus 2, Gallid / growth & development; Herpesvirus 2, Gallid / immunology; Immunologic Factors / metabolism; Ligands; Toll-Like Receptors / metabolism
TL;DR: Investigation of the stimulatory effect of various PAMPs (in the form of TLR ligands) in reducing Marek's disease virus (MDV) infection in chicken embryo fibroblast cells (CEFs) indicated that pretreatment with Poly(IC) resulted in a robust reduction of MDV infection in CEFs at 96 h postinfection while a moderate reduction was observed with treatment of Pam3CSK4, LPS, and CpG ODN. (via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being (OpenAlex)
Sources: Crossref, NC State University Libraries
Added: September 8, 2019

2017 journal article

CpG oligonucleotide-mediated co-stimulation of mouse invariant natural killer T cells negatively regulates their activation status

Cell and Tissue Research, 369(3), 541–554.

By: R. Kulkarni*, A. Villanueva*, L. Read*, J. Brisbin*, S. Bhaumik*, J. LaMarre*, K. Murali-Krishna*, S. Sharif*

author keywords: Invariant NKT cells; Innate lymphocytes; Toll-like receptor; CpG oligonucleotides; Immune regulation
MeSH headings : Animals; Antibodies, Monoclonal / pharmacology; CD3 Complex / metabolism; Down-Regulation / drug effects; Galactosylceramides / pharmacology; Interferon-gamma / metabolism; Lymphocyte Activation / drug effects; MAP Kinase Signaling System / drug effects; Mice, Inbred C57BL; Myeloid Differentiation Factor 88 / metabolism; Natural Killer T-Cells / drug effects; Natural Killer T-Cells / immunology; Oligodeoxyribonucleotides / pharmacology; Phosphoprotein Phosphatases / metabolism; Receptors, Antigen, T-Cell / metabolism; Toll-Like Receptor 9 / genetics; Toll-Like Receptor 9 / metabolism; Transcription, Genetic / drug effects; Up-Regulation / drug effects
TL;DR: A novel regulatory role for CpG in iNKT cells is suggested in the mediation of a negative feedback mechanism to control overactive iN KT cell responses and hence to avoid undesirable excessive immunopathology. (via Semantic Scholar)
Sources: Crossref, NC State University Libraries
Added: September 8, 2019

2016 journal article

Delivery of an inactivated avian influenza virus vaccine adjuvanted with poly(D,L-lactic-co-glycolic acid) encapsulated CpG ODN induces protective immune responses in chickens

Vaccine, 34(40), 4807–4813.

By: S. Singh*, T. Alkie*, É. Nagy*, R. Kulkarni*, D. Hodgins* & S. Sharif*

author keywords: Avian influenza virus; Chickens; CpG ODN; Poly(D,L-lactic-co-glycolic acid); Delivery system; Nanoparticle; Immune response
MeSH headings : Adjuvants, Immunologic / administration & dosage; Animals; Antibodies, Viral / blood; Antibody Affinity; Chickens / immunology; Immunity, Mucosal; Immunoglobulin A / immunology; Immunoglobulin M / immunology; Immunoglobulins / immunology; Influenza A Virus, H9N2 Subtype; Influenza Vaccines / immunology; Influenza Vaccines / therapeutic use; Influenza in Birds / prevention & control; Lactic Acid / administration & dosage; Nanoparticles / administration & dosage; Oligodeoxyribonucleotides / immunology; Polyglycolic Acid / administration & dosage; Polylactic Acid-Polyglycolic Acid Copolymer; Poultry Diseases / prevention & control; Vaccines, Inactivated / immunology; Vaccines, Inactivated / therapeutic use; Virus Shedding
TL;DR: Collectively, the data indicate that vaccination of chickens with nonencapsulated AIV plus PLGA encapsulated CpG 2007 results in qualitatively and quantitatively augmented antibody responses leading to a reduction in virus shedding compared to the encapsulated Aiv plus PLG 2007 formulation. (via Semantic Scholar)
Sources: Crossref, NC State University Libraries
Added: September 8, 2019

2015 journal article

Modulation of cytokine gene expression by selected Lactobacillus isolates in the ileum, caecal tonsils and spleen of Salmonella-challenged broilers

Avian Pathology, 44(6), 463–469.

By: J. Hu*, H. Yu*, R. Kulkarni, S. Sharif, S. Cui*, M. Xie*, S. Nie*, J. Gong*

MeSH headings : Animals; Cecum / immunology; Chickens; Cytokines / genetics; Female; Ileum / immunology; Lactobacillus / physiology; Liver / immunology; Poultry Diseases / immunology; Poultry Diseases / microbiology; Poultry Diseases / prevention & control; Salmonella Infections, Animal / immunology; Salmonella Infections, Animal / microbiology; Salmonella Infections, Animal / prevention & control; Salmonella typhimurium / physiology; Spleen / immunology
TL;DR: Results show that administration of certain selected Lactobacillus isolates can effectively modulate Salmonella-induced cytokine gene expression, and thus help reduceSalmonella infection in chickens. (via Semantic Scholar)
Sources: Crossref, NC State University Libraries
Added: January 5, 2020

2014 journal article

Activation of the RIG-I Pathway during Influenza Vaccination Enhances the Germinal Center Reaction, Promotes T Follicular Helper Cell Induction, and Provides a Dose-Sparing Effect and Protective Immunity

Journal of Virology, 88(24), 13990–14001.

By: R. Kulkarni*, M. Rasheed*, S. Bhaumik*, P. Ranjan*, W. Cao*, C. Davis*, K. Marisetti*, S. Thomas* ...

MeSH headings : Adjuvants, Immunologic / administration & dosage; Animals; Antibodies, Viral / blood; Cell Proliferation; DEAD Box Protein 58; DEAD-box RNA Helicases / metabolism; Disease Models, Animal; Germinal Center / immunology; Hemagglutination Inhibition Tests; Influenza Vaccines / administration & dosage; Influenza Vaccines / immunology; Mice, Inbred BALB C; Mice, Inbred C57BL; Orthomyxoviridae Infections / immunology; Orthomyxoviridae Infections / prevention & control; RNA, Double-Stranded / administration & dosage; Signal Transduction; T-Lymphocytes, Helper-Inducer / immunology; T-Lymphocytes, Helper-Inducer / physiology; Vaccination / methods
TL;DR: This study shows that the RLR pathway can be effectively exploited to enhance adaptive immunity and protective immune memory against viral infection and indicates the potential utility of RIG-I ligands as molecular adjuvants for viral vaccines. (via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being (OpenAlex)
Sources: Crossref, NC State University Libraries
Added: January 5, 2020

2014 journal article

Functions of invariant NK T cells are modulated by TLR ligands and IFN-α

Innate Immunity, 21(3), 275–288.

author keywords: TLR; IFN-; iNKT cell; macrophage; antiviral; CpG
MeSH headings : Animals; Cell Line; Culture Media, Conditioned / pharmacology; Cytokines / metabolism; Fibroblasts / drug effects; Fibroblasts / immunology; Fibroblasts / virology; Interferon-alpha / immunology; Ligands; Lymphocyte Activation; Macrophages / drug effects; Macrophages / immunology; Mice; Natural Killer T-Cells; Rhabdoviridae Infections / immunology; Toll-Like Receptors / agonists; Toll-Like Receptors / genetics; Toll-Like Receptors / metabolism; Vesiculovirus / physiology; Virus Replication / drug effects
TL;DR: The role of IFN-α in iNKT cell activation, as well as the direct modulatory effects of TLR ligands on iN KT cell function, including antiviral activity are demonstrated. (via Semantic Scholar)
Sources: Crossref, NC State University Libraries
Added: January 5, 2020

2012 journal article

Costimulatory activation of murine invariant natural killer T cells by toll-like receptor agonists

Cellular Immunology, 277(1-2), 33–43.

By: R. Kulkarni*, A. Villanueva*, I. Elawadli*, P. Jayanth*, L. Read*, S. Mansour Haeryfar*, S. Sharif*

author keywords: CD1d; Innate immunity; T cell receptors; alpha-GalCer; CD3 stimulation
MeSH headings : Animals; Antibodies, Neutralizing / immunology; CD3 Complex / immunology; Cell Line; Cytokines / biosynthesis; Cytokines / immunology; Dendritic Cells / drug effects; Dendritic Cells / immunology; Galactosylceramides / pharmacology; Gene Expression Regulation / drug effects; Lymphocyte Activation / drug effects; Lymphocyte Activation / immunology; Mice; Natural Killer T-Cells / drug effects; Natural Killer T-Cells / immunology; Receptors, Antigen, T-Cell / immunology; Toll-Like Receptors / agonists; Toll-Like Receptors / biosynthesis; Toll-Like Receptors / immunology
TL;DR: It is shown that TCR-activation of murine iNKT cells by α-GalactosylCeramide (α-GalCer) or anti-CD3 antibodies can result in increased expression of TLR genes, and a subsequent TLR-signaling in these cells can lead to their enhanced activation, suggesting a new possible mode of iN KT cell activation. (via Semantic Scholar)
Sources: Crossref, NC State University Libraries
Added: January 5, 2020

2012 journal article

Vaccination with CpG-Adjuvanted Avian Influenza Virosomes Promotes Antiviral Immune Responses and Reduces Virus Shedding in Chickens

Viral Immunology, 4, 120418071632002.

MeSH headings : Adjuvants, Immunologic / administration & dosage; Animals; Antibodies, Neutralizing / blood; Antibodies, Viral / blood; Chickens / immunology; Influenza A virus / immunology; Influenza Vaccines / administration & dosage; Influenza in Birds / immunology; Influenza in Birds / prevention & control; Influenza in Birds / virology; Oligodeoxyribonucleotides / administration & dosage; Oligodeoxyribonucleotides / immunology; Poultry Diseases / immunology; Poultry Diseases / prevention & control; Vaccination; Vaccines, Virosome / administration & dosage; Vaccines, Virosome / immunology; Virus Shedding / immunology
TL;DR: The ability of virosomes adjuvanted with CpG-ODN to reduce AIV shedding, and elicit virus-specific protective antibody responses in vaccinated birds is demonstrated. (via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being (OpenAlex)
Sources: Crossref, NC State University Libraries
Added: January 5, 2020

2011 journal article

Assessment of Bioactivity of a Recombinant Chicken Interferon-Gamma Expressed Using a Baculovirus Expression System

Journal of Interferon & Cytokine Research, 31(6), 493–500.

MeSH headings : Animals; Avian Proteins / genetics; Avian Proteins / metabolism; Avian Proteins / pharmacology; Baculoviridae / genetics; Cell Line; Chick Embryo; Chickens; Cloning, Molecular; Cytokines / biosynthesis; Cytokines / genetics; Feasibility Studies; Fibroblasts / immunology; Fibroblasts / metabolism; Fibroblasts / pathology; Gene Expression Regulation / drug effects; Gene Expression Regulation / immunology; Genetic Vectors / genetics; Glycosylation; Histocompatibility Antigens Class II / biosynthesis; Histocompatibility Antigens Class II / genetics; Interferon-gamma / genetics; Interferon-gamma / metabolism; Interferon-gamma / pharmacology; Macrophages / immunology; Macrophages / metabolism; Macrophages / pathology; Nitric Oxide Synthase Type II / metabolism; Recombinant Proteins; STAT1 Transcription Factor / biosynthesis; STAT1 Transcription Factor / genetics
TL;DR: Information is provided on the ability of functionally active rChIFN-γ expressed in a baculovirus system in inducing significant transcriptional upregulation of various immune system-related genes, including those that encode cytokines, antigen-presenting molecules, and transcription factors involved in the major histocompatibility complex and IFN-signaling pathway. (via Semantic Scholar)
Sources: Crossref, NC State University Libraries
Added: January 5, 2020

2011 journal article

Synthetic double-stranded RNA oligonucleotides are immunostimulatory for chicken spleen cells

Developmental & Comparative Immunology, 35(1), 28–34.

By: A. Villanueva*, R. Kulkarni* & S. Sharif*

author keywords: Toll-like receptor; Type I interferon; TLR3; TLR7; dsRNA; siRNA; Chicken
MeSH headings : Adjuvants, Immunologic / pharmacology; Animals; Cells / drug effects; Cells, Cultured; Chickens; Gene Expression Regulation / drug effects; Immunization; Oligonucleotides / pharmacology; RNA, Double-Stranded / pharmacology; Reverse Transcriptase Polymerase Chain Reaction; Signal Transduction / drug effects; Spleen / cytology; Toll-Like Receptor 3 / immunology; Toll-Like Receptor 7 / immunology
TL;DR: The present study demonstrated the immunostimulatory properties of dsRNA oligonucleotides, especially those that contain a poly-UG motif, in chickens. (via Semantic Scholar)
Sources: Crossref, NC State University Libraries
Added: January 5, 2020

2010 journal article

Assessment of 2 Salmonella enterica serovar Typhimurium-based vaccines against necrotic enteritis in reducing colonization of chickens by Salmonella serovars of different serogroups

Canadian Journal of Veterinary Research, 74(4), 264–270.

By: Y. Jiang, R. Kulkarni, V. Parreira, C. Poppe, K. Roland & J. Prescott

Source: NC State University Libraries
Added: February 13, 2021

2010 journal article

Insights into the role of Toll-like receptors in modulation of T cell responses

Cell and Tissue Research, 343(1), 141–152.

By: R. Kulkarni*, S. Behboudi* & S. Sharif*

author keywords: T cells; Toll-like receptors; Innate immunity; Immune response; Mucosal immunity
MeSH headings : Animals; Autoimmunity / immunology; Humans; Immunity, Mucosal / immunology; Lymphocyte Activation / immunology; Receptors, Antigen, T-Cell, gamma-delta / immunology; T-Lymphocytes / cytology; T-Lymphocytes / immunology; Toll-Like Receptors / agonists; Toll-Like Receptors / immunology
TL;DR: The present review attempts to summarize the recent findings related to TLR expression in different T cell subsets and the direct role of TLRs in the induction and regulation of T cell responses, including those responses that occur at mucosal surfaces. (via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being (OpenAlex)
Sources: Crossref, NC State University Libraries
Added: January 5, 2020

2010 journal article

The invariant NKT cell subset in anti-viral defenses: a dark horse in anti-influenza immunity?

Journal of Leukocyte Biology, 88(4), 635–643.

By: R. Kulkarni*, S. Haeryfar* & S. Sharif*

author keywords: CD1d; viral immunology; influenza virus; glycolipids; Toll-like receptors
MeSH headings : Animals; Humans; Lymphocyte Activation / immunology; Natural Killer T-Cells / immunology; Orthomyxoviridae / immunology; Orthomyxoviridae Infections / immunology; Virus Diseases / immunology
TL;DR: A review of studies that have investigated the role of iNKT cells in immune responses against influenza virus have suggested an important role for these cells in innate defense mechanisms as well as antibody‐ and cell‐mediated responses. (via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being (OpenAlex)
Sources: Crossref, NC State University Libraries
Added: January 5, 2020

2009 journal article

A Live Oral Recombinant Salmonella enterica Serovar Typhimurium Vaccine Expressing Clostridium perfringens Antigens Confers Protection against Necrotic Enteritis in Broiler Chickens

Clinical and Vaccine Immunology, 17(2), 205–214.

By: R. Kulkarni*, V. Parreira*, Y. Jiang* & J. Prescott*

MeSH headings : Administration, Oral; Animals; Antibodies, Bacterial / blood; Antigens, Bacterial / genetics; Antigens, Bacterial / immunology; Bacterial Toxins / genetics; Bacterial Toxins / immunology; Bacterial Vaccines / administration & dosage; Bacterial Vaccines / genetics; Bacterial Vaccines / immunology; Body Weight; Calcium-Binding Proteins / genetics; Calcium-Binding Proteins / immunology; Chickens; Cloning, Molecular; Clostridium Infections / prevention & control; Clostridium Infections / veterinary; Clostridium perfringens / genetics; Clostridium perfringens / immunology; Enteritis / pathology; Enteritis / prevention & control; Enteritis / veterinary; Epitopes, B-Lymphocyte; Genetic Vectors; Immunoglobulin A / blood; Immunoglobulins / blood; Male; Plasmids; Poultry Diseases / microbiology; Poultry Diseases / pathology; Poultry Diseases / prevention & control; Salmonella typhimurium / genetics; Severity of Illness Index; Type C Phospholipases / genetics; Type C Phospholipases / immunology
TL;DR: The B-cell epitopes in AT from an NE isolate recognized by chickens are identified for the first time and the partial protective ability of codon-optimized ATd and HPt against NE is shown in broiler chickens when they were delivered orally by using a Salmonella vaccine vehicle. (via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being (OpenAlex)
Sources: Crossref, NC State University Libraries
Added: January 5, 2020

2009 journal article

Immunization of Broiler Chickens Against Clostridium perfringens–Induced Necrotic Enteritis Using Purified Recombinant Immunogenic Proteins

Avian Diseases, 53(3), 409–415.

author keywords: immunization; broiler chickens; Clostridium perfringens; necrotic enteritis; endo-beta-N-acetylglucosaminidase; phosphoglyceromutase
MeSH headings : Animals; Antibodies, Bacterial / blood; Bacterial Proteins / genetics; Bacterial Proteins / immunology; Bacterial Proteins / metabolism; Bacterial Vaccines / immunology; Chickens; Cloning, Molecular; Clostridium Infections / prevention & control; Clostridium Infections / veterinary; Clostridium perfringens / immunology; Enteritis / microbiology; Enteritis / prevention & control; Enteritis / veterinary; Gene Expression Regulation, Bacterial / physiology; Poultry Diseases / prevention & control; Recombinant Proteins / immunology
TL;DR: This study demonstrated the partial efficacy of additional secreted proteins in immunity of broiler chickens to NE and showed that there may be differences in the protective ability of immunogens depending on the infecting C. perfringens strain. (via Semantic Scholar)
Sources: Crossref, NC State University Libraries
Added: January 5, 2020

2008 journal article

Multilocus Sequence Typing Analysis of Clostridium perfringens Isolates from Necrotic Enteritis Outbreaks in Broiler Chicken Populations

Journal of Clinical Microbiology, 46(12), 3957–3964.

By: G. Chalmers*, H. Bruce*, D. Hunter*, V. Parreira*, R. Kulkarni*, Y. Jiang*, J. Prescott*, P. Boerlin*

MeSH headings : Animals; Anti-Bacterial Agents / pharmacology; Bacitracin / pharmacology; Bacterial Toxins / genetics; Bacterial Typing Techniques / methods; Birds; Chickens; Clostridium Infections / epidemiology; Clostridium Infections / microbiology; Clostridium Infections / veterinary; Clostridium perfringens / classification; Clostridium perfringens / genetics; Clostridium perfringens / isolation & purification; Cluster Analysis; DNA Fingerprinting / methods; DNA, Bacterial / chemistry; DNA, Bacterial / genetics; Disease Outbreaks; Drug Resistance, Bacterial; Enteritis / epidemiology; Enteritis / microbiology; Enteritis / veterinary; Genotype; Ontario / epidemiology; Poultry Diseases / epidemiology; Poultry Diseases / microbiology; Sequence Analysis, DNA; Virulence Factors / genetics
TL;DR: This study focuses on the typing of intestinal C. perfringens isolates from outbreaks of NE collected from several areas of Southern Ontario, using a recently developed multilocus sequence typing (MLST) technique. (via Semantic Scholar)
UN Sustainable Development Goal Categories
10. Reduced Inequalities (OpenAlex)
Sources: Crossref, NC State University Libraries
Added: January 5, 2020

2008 journal article

Oral immunization of broiler chickens against necrotic enteritis with an attenuated Salmonella vaccine vector expressing Clostridium perfringens antigens

Vaccine, 26(33), 4194–4203.

By: R. Kulkarni*, V. Parreira*, S. Sharif* & J. Prescott*

author keywords: Clostrdium perfringens; vaccine; necrotic enteritis; chickens
MeSH headings : Administration, Oral; Animals; Antibodies, Bacterial / analysis; Antibodies, Bacterial / blood; Antigens, Bacterial / genetics; Antigens, Bacterial / immunology; Bacterial Proteins / genetics; Bacterial Proteins / immunology; Body Weight; Chickens; Clostridium Infections / prevention & control; Clostridium perfringens / genetics; Clostridium perfringens / immunology; Enteritis / prevention & control; Enteritis / veterinary; Fructose-Bisphosphate Aldolase / genetics; Fructose-Bisphosphate Aldolase / immunology; Intestines / immunology; Intestines / pathology; Poultry Diseases / prevention & control; Pyruvate Synthase / genetics; Pyruvate Synthase / immunology; Salmonella Vaccines / immunology; Salmonella typhimurium / genetics; Vaccines, Attenuated / immunology; Vaccines, Synthetic / immunology
TL;DR: This study showed the oral immunizing ability of two C. perfringens antigens against NE in broiler chickens through an attenuated Salmonella vaccine vector. (via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being (OpenAlex)
Sources: Crossref, NC State University Libraries
Added: January 5, 2020

2007 journal article

Immunization of Broiler Chickens against Clostridium perfringens-Induced Necrotic Enteritis

Clinical and Vaccine Immunology, 14(9), 1070–1077.

By: R. Kulkarni*, V. Parreira*, S. Sharif* & J. Prescott*

MeSH headings : Animals; Antibodies, Bacterial / blood; Antibodies, Bacterial / immunology; Bacterial Toxins / biosynthesis; Bacterial Toxins / genetics; Bacterial Toxins / immunology; Bacterial Vaccines / administration & dosage; Bacterial Vaccines / immunology; Calcium-Binding Proteins / biosynthesis; Calcium-Binding Proteins / genetics; Calcium-Binding Proteins / immunology; Chickens / immunology; Cloning, Molecular; Clostridium Infections / immunology; Clostridium Infections / microbiology; Clostridium Infections / prevention & control; Clostridium Infections / veterinary; Clostridium perfringens / enzymology; Clostridium perfringens / genetics; Clostridium perfringens / immunology; Enteritis / immunology; Enteritis / pathology; Enteritis / prevention & control; Enteritis / veterinary; Fructose-Bisphosphate Aldolase / biosynthesis; Fructose-Bisphosphate Aldolase / genetics; Fructose-Bisphosphate Aldolase / immunology; Glyceraldehyde-3-Phosphate Dehydrogenases / biosynthesis; Glyceraldehyde-3-Phosphate Dehydrogenases / genetics; Glyceraldehyde-3-Phosphate Dehydrogenases / immunology; Intestine, Small / immunology; Male; Necrosis; Poultry Diseases / immunology; Poultry Diseases / microbiology; Poultry Diseases / prevention & control; Pyruvate Synthase / biosynthesis; Pyruvate Synthase / genetics; Pyruvate Synthase / immunology; Recombinant Proteins / biosynthesis; Recombinant Proteins / genetics; Recombinant Proteins / immunology; Type C Phospholipases / biosynthesis; Type C Phospholipases / genetics; Type C Phospholipases / immunology
TL;DR: There are certain secreted proteins, in addition to alpha-toxin, that are involved in immunity to NE in broiler chickens, which are concluded to be uniquely produced by virulent C. perfringens. (via Semantic Scholar)
Sources: Crossref, NC State University Libraries
Added: January 5, 2020

2007 journal article

Regulation of the intestinal immune system by commensal microbiota in avian and ruminant species

CAB Reviews: Perspectives in Agriculture, Veterinary Science, Nutrition and Natural Resources, 2(8), 11–19.

By: J. Brisbin, H. Haghighi, R. Kulkarni & S. Sharif

Source: NC State University Libraries
Added: February 13, 2021

2006 journal article

Clostridium perfringens Antigens Recognized by Broiler Chickens Immune to Necrotic Enteritis

Clinical and Vaccine Immunology, 13(12), 1358–1362.

By: R. Kulkarni*, V. Parreira*, S. Sharif* & J. Prescott*

MeSH headings : Animals; Antigens, Bacterial / immunology; Antigens, Bacterial / metabolism; Bacterial Proteins / immunology; Bacterial Proteins / metabolism; Chickens / immunology; Clostridium Infections / immunology; Clostridium Infections / microbiology; Clostridium Infections / veterinary; Clostridium perfringens / immunology; Clostridium perfringens / pathogenicity; Enteritis / immunology; Enteritis / veterinary; Immune Sera / immunology; Necrosis / immunology; Necrosis / veterinary; Poultry Diseases / immunology; Poultry Diseases / microbiology; Virulence
TL;DR: Six secreted antigenic proteins unique to virulent C. perfringens that reacted to serum antibodies from immune birds were identified by mass spectrophotometry; three of these proteins are part of the VirR-VirS regulon. (via Semantic Scholar)
Sources: Crossref, NC State University Libraries
Added: January 5, 2020

2006 journal article

Live attenuated vaccine-based control of necrotic enteritis of broiler chickens

Veterinary Microbiology, 113(1-2), 25–34.

By: D. Thompson*, V. Parreira*, R. Kulkarni* & J. Prescott*

author keywords: Clostridium perfringens; necrotic enteritis; vaccination; alpha-toxin mutants
MeSH headings : Animals; Anti-Bacterial Agents / therapeutic use; Bacitracin / therapeutic use; Bacterial Vaccines; Chickens; Clostridium Infections / prevention & control; Clostridium Infections / veterinary; Clostridium perfringens / drug effects; Clostridium perfringens / immunology; Clostridium perfringens / pathogenicity; DNA Primers / chemistry; Electrophoresis, Gel, Pulsed-Field / veterinary; Enteritis / prevention & control; Enteritis / veterinary; Escherichia coli / genetics; Genotype; Molecular Sequence Data; Mutation / genetics; Necrosis; Poultry Diseases / pathology; Poultry Diseases / prevention & control; Reverse Transcriptase Polymerase Chain Reaction / veterinary; Sequence Analysis, DNA; Type C Phospholipases / biosynthesis; Type C Phospholipases / genetics; Type C Phospholipases / immunology; Vaccines, Attenuated; Virulence
TL;DR: It is concluded that it is possible to immunize chickens successfully against necrotic enteritis and that immunogen(s) other than alpha-toxin are important in protective immunity against oral infection. (via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being (OpenAlex)
Sources: Crossref, NC State University Libraries
Added: January 5, 2020

1999 journal article

Development of a precocious strain of Eimeria tenella: Preliminary observations

Journal of Veterinary Parasitology, 13(2), 107–110.

By: J. Rao, A. Mishra & R. Kulkarni

Source: NC State University Libraries
Added: February 13, 2021

1999 journal article

Eimeria tenella: Isolation, purification and protein profile of merozoite and oocysts wall proteins of a strain of Eimeria tenella selected for precociousness

Journal of Veterinary Parasitology, 13(2), 119–123.

By: R. Kulkarni, J. Rao, S. Omanwar & R. Singh

Source: NC State University Libraries
Added: February 13, 2021

Employment

Updated: December 31st, 2023 12:47

2019 - present

North Carolina State University Raleigh, NC, US
Assistant Professor Population Health and Pathobiology

2015 - 2018

University of Guelph Guelph, ON, CA
PHRN Coordinator and Associate Graduate Faculty Pathobiology

2011 - 2014

Emory University Atlanta, GA, US
Postdoc and Research Associate Emory Vaccine Center

Education

Updated: December 31st, 2023 12:48

Indian Veterinary Research Institute Bareilly, IN
Masters (MVSc)

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