@article{lund_hall_williams_2019, title={An Artificial Pathway for Isoprenoid Biosynthesis Decoupled from Native Hemiterpene Metabolism}, volume={8}, ISSN={2161-5063 2161-5063}, url={http://dx.doi.org/10.1021/acssynbio.8b00383}, DOI={10.1021/acssynbio.8b00383}, abstractNote={Isoprenoids are constructed in nature using hemiterpene building blocks that are biosynthesized from lengthy enzymatic pathways with little opportunity to deploy precursor-directed biosynthesis. Here, an artificial alcohol-dependent hemiterpene biosynthetic pathway was designed and coupled to several isoprenoid biosynthetic systems, affording lycopene and a prenylated tryptophan in robust yields. This approach affords a potential route to diverse non-natural hemiterpenes and by extension isoprenoids modified with non-natural chemical functionality. Accordingly, the prototype chemo-enzymatic pathway is a critical first step toward the construction of engineered microbial strains for bioconversion of simple scalable building blocks into complex isoprenoid scaffolds.}, number={2}, journal={ACS Synthetic Biology}, publisher={American Chemical Society (ACS)}, author={Lund, Sean and Hall, Rachael and Williams, Gavin J}, year={2019}, month={Jan}, pages={232–238} }