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Works (31)
Updated: April 5th, 2024 03:49
2023 journal article
Modified physiology of burley tobacco plants genetically engineered to express Yb<sub>1</sub>, a functional EGY enzyme
PLANTA, 258(4).
author keywords: Burley tobacco; Gene editing; Nicotiana tabacum; Nitrogen use efficiency; RNA-sequencing
TL;DR:
Results indicate that complex changes in the transcriptome of tobacco can be manifested by altered expression of Yb 1, and metabolic pathways affecting carbon and nitrogen metabolism that might be altered as the result of genetic variability at the Yb1 and Yb2 loci.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger
(Web of Science)
3. Good Health and Well-being
(OpenAlex)
Source: Web Of Science
Added: October 23, 2023
2021 journal article
Enzyme Complexes of Ptr4CL and PtrHCT Modulate Co-enzyme A Ligation of Hydroxycinnamic Acids for Monolignol Biosynthesis in Populus trichocarpa
FRONTIERS IN PLANT SCIENCE, 12.
author keywords: protein interaction; monolignol biosynthesis; wood formation; Populus trichocarpa; BiFC; metabolic flux
TL;DR:
It is proposed that Ptr4CL/PtrHCT complexes modulate the metabolic flux of CoA ligation for monolignol biosynthesis during wood formation in P. trichocarpa.
(via Semantic Scholar)
www.frontiersin.org (publisher PDF)
Sources: Web Of Science, NC State University Libraries, ORCID
Added: November 8, 2021
2021 article
Homoeologous chromosome exchange explains the creation of a QTL affecting soil-borne pathogen resistance in tobacco
Shi, R., Jin, J., Nifong, J. M., Shew, D., & Lewis, R. S. (2021, October 22). PLANT BIOTECHNOLOGY JOURNAL.
author keywords: homoeologous exchange; QTL; resistance; SAR8; 2; tobacco; Phytophthora
MeSH headings : Chromosomes, Plant / genetics; Disease Resistance / genetics; Plant Diseases / genetics; Quantitative Trait Loci / genetics; Soil; Tobacco / genetics
TL;DR:
A role for genomic rearrangements in the generation of favourable genetic variability affecting resistance to pathogens in plants is suggested.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger
(Web of Science)
3. Good Health and Well-being
(OpenAlex)
13. Climate Action
(Web of Science)
Source: Web Of Science
Added: November 1, 2021
2021 journal article
RNAseq Reveals Differential Gene Expression Contributing to Phytophthora nicotianae Adaptation to Partial Resistance in Tobacco
AGRONOMY-BASEL, 11(4).
author keywords: RNAseq; Phytophthora nicotianae; adaptation; partial resistance; tobacco
TL;DR:
Understanding of the molecular mechanisms of P. nicotianae adaptation to partial resistance in tobacco is enhanced by elucidating how the pathogen recruits pathogenicity-associated genes that impact host biological activities.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger
(Web of Science)
3. Good Health and Well-being
(OpenAlex)
13. Climate Action
(Web of Science)
Source: Web Of Science
Added: May 10, 2021
2019 journal article
Genetic Control of Facultative Parthenocarpy in Nicotiana tabacum L.
JOURNAL OF HEREDITY, 110(5), 610–617.
author keywords: Genomics and gene mapping; haploids; Nicotiana; parthenocarpy; QTL mapping; tobacco
MeSH headings : Chromosome Mapping; Fruit / genetics; Genetic Association Studies; Haploidy; Parthenogenesis / genetics; Quantitative Trait Loci; Quantitative Trait, Heritable; Seeds / genetics; Tobacco / genetics
TL;DR:
Investigation of parthenocarpy, the production of fruit without fertilization, in multiple plant species could result in development of technologies for conferring seedless fruits and increased stability of fruit formation in economically important plants.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger
(Web of Science)
3. Good Health and Well-being
(OpenAlex)
13. Climate Action
(Web of Science)
Source: Web Of Science
Added: September 16, 2019
2019 journal article
Identification and validation of SNP markers associated with Wz-mediated Phytophthora nicotianae resistance in Nicotiana tabacum L.
MOLECULAR BREEDING, 39(7).
author keywords: Nicotiana; Tobacco; RNA-seq; Black shank; Phytophthora nicotianae
TL;DR:
The utility of a subset of derived Kompetitive Allele Specific PCR (KASP) and Cleaved Amplified Polymorphic Sequence (CAPS) markers for predicting the presence of Wz was verified using segregating populations and F3:4 families that were evaluated for field black shank resistance.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(OpenAlex)
Source: Web Of Science
Added: July 22, 2019
2018 journal article
Filter paper-based spin column method for cost-efficient DNA or RNA purification
PLOS ONE, 13(12).
MeSH headings : Centrifugation / methods; DNA, Plant / chemistry; DNA, Plant / isolation & purification; Solanum lycopersicum / chemistry; Paper; RNA, Plant / chemistry; RNA, Plant / isolation & purification; Tobacco / chemistry
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: December 31, 2018
2018 journal article
Improving wood properties for wood utilization through multi-omics integration in lignin biosynthesis
Nature Communications, 9(1), 1579.
Contributors: J. Wang n , M. Matthews n, C. Williams n , n, C. Yang n, S. Tunlaya-Anukit n, H. Chen n, Q. Li *
MeSH headings : Gene Expression Regulation, Plant; Lignin / biosynthesis; Lignin / genetics; Plants, Genetically Modified / genetics; Populus / genetics; Populus / metabolism; Transcriptome / genetics; Trees / genetics; Trees / metabolism; Wood / chemistry; Wood / physiology; Xylem / metabolism
TL;DR:
A multi-omics quantitative integrative analysis of lignin biosynthesis can advance the strategic engineering of wood for timber, pulp, and biofuels and may lead to greater understanding of other pathways for improved growth and adaptation.
(via Semantic Scholar)
Sources: Web Of Science, ORCID, NC State University Libraries, Crossref
Added: August 6, 2018
2017 journal article
A novel plant DNA extraction method using filter paper-based 96-well spin plate
Planta, 246(3), 579–584.
author keywords: Filter paper; DNA extraction; Inexpensive; Safe; High throughput; Solanum lycopersicum; Ph-3 gene
MeSH headings : Centrifugation / instrumentation; Centrifugation / methods; DNA, Plant / isolation & purification; Electrophoresis / methods; Genetic Markers; Polymerase Chain Reaction; Reproducibility of Results
TL;DR:
It was confirmed that filter paper is an efficient solid-phase DNA binding material and comparable to silicon-based glass fiber filters adopted in commercial DNA extraction kits, and that plant DNA extracted by this method can be readily used as template for PCR.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger
(Web of Science)
13. Climate Action
(Web of Science)
15. Life on Land
(Web of Science)
Sources: Web Of Science, NC State University Libraries, Crossref
Added: August 6, 2018
2017 journal article
Reciprocal cross-regulation of VND and SND multigene TF families for wood formation in Populus trichocarpa
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 114(45), E9722–E9729.
Contributors: Y. Lin n, H. Chen n, Q. Li *, W. Li n, J. Wang n , n, S. Tunlaya-Anukit n, P. Shuai n
author keywords: reciprocal cross-regulation; NAC transcription factors; alternative splicing; wood formation; Populus trichocarpa
MeSH headings : Alternative Splicing; Cell Wall / genetics; Cell Wall / metabolism; Cloning, Molecular; DNA, Plant; Gene Expression Regulation, Plant / genetics; Gene Regulatory Networks; Genes, Plant; Homeostasis; Multigene Family; Nuclear Proteins; Plant Leaves / genetics; Plant Leaves / metabolism; Plant Proteins / genetics; Plant Proteins / metabolism; Plant Shoots / genetics; Plant Shoots / metabolism; Populus / genetics; Populus / metabolism; Recombinant Proteins / genetics; Transcription Factors / genetics; Transcription Factors / metabolism; Transcriptional Activation / genetics; Transcriptome; Wood / genetics; Wood / growth & development; Xylem / genetics; Xylem / growth & development
TL;DR:
The results indicate that the splice variants from the PtrVND6 and PtrSND1 family may exert reciprocal cross-regulation for complete transcriptional regulation of these two families in wood formation, suggesting a general mechanism among NAC domain proteins and likely other transcription factors.
(via Semantic Scholar)
Sources: Web Of Science, ORCID
Added: August 6, 2018
2017 journal article
Tissue and cell-type co-expression networks of transcription factors and wood component genes in Populus trichocarpa
PLANTA, 245(5), 927–938.
author keywords: Wood formation; Transcriptome; Fiber cells; Vessel elements; Cell wall biosynthesis; Co-expression network
MeSH headings : Cell Wall / metabolism; Cellulose / metabolism; Cluster Analysis; Gene Expression Regulation, Plant; Lignin / metabolism; Molecular Sequence Annotation; Organ Specificity; Phloem / genetics; Phloem / growth & development; Plant Leaves / genetics; Plant Leaves / growth & development; Plant Proteins / genetics; Populus / genetics; Populus / growth & development; Sequence Analysis, RNA; Transcription Factors / genetics; Transcriptome; Wood / genetics; Wood / growth & development; Xylem / genetics; Xylem / growth & development
TL;DR:
The co-expression networks revealed here suggest a well-structured transcriptional homeostasis for cell wall component biosynthesis during wood formation.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger
(OpenAlex)
Sources: Web Of Science, ORCID
Added: August 6, 2018
2016 journal article
A cell wall-bound anionic peroxidase, PtrPO21, is involved in lignin polymerization in Populus trichocarpa
Tree Genetics & Genomes, 12(2).
author keywords: Lignin polymerization; Populus trichocarpa; Lignin peroxidase; LC-MS/MS; Lignin systems biology
TL;DR:
Differentially expressed gene (DEG) analysis of PtrPO21 downregulated transgenics identified a significant overexpression of PtPrx35, suggesting a compensatory effect within the peroxidase family.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
15. Life on Land
(OpenAlex)
Sources: Web Of Science, ORCID, Crossref, NC State University Libraries
Added: August 6, 2018
2015 journal article
Phosphorylation is an on/off switch for 5-hydroxyconiferaldehyde O-methyltransferase activity in poplar monolignol biosynthesis
Proceedings of the National Academy of Sciences, 112(27), 8481–8486.
Contributors: J. Wang n , L. Chuang n, P. Loziuk n, H. Chen n, Y. Lin n, n, G. Qu *, D. Muddiman n , R. Sederoff n, V. Chiang n
author keywords: AldOMT; COMT; lignin; phosphoproteomics; phosphoregulation
MeSH headings : Acrolein / analogs & derivatives; Acrolein / metabolism; Amino Acid Sequence; Binding Sites / genetics; Biocatalysis; Catechols / metabolism; Chromatography, Liquid; Electrophoresis, Polyacrylamide Gel; Green Fluorescent Proteins / genetics; Green Fluorescent Proteins / metabolism; Isoenzymes / genetics; Isoenzymes / metabolism; Lignin / biosynthesis; Methyltransferases / genetics; Methyltransferases / metabolism; Microscopy, Confocal; Molecular Sequence Data; Mutation; Phosphoproteins / metabolism; Phosphorylation; Plant Proteins / genetics; Plant Proteins / metabolism; Populus / enzymology; Populus / genetics; Populus / metabolism; Proteomics / methods; Protoplasts / enzymology; Protoplasts / metabolism; Sequence Homology, Amino Acid; Tandem Mass Spectrometry
TL;DR:
It is shown that reversible monophosphorylation at Ser123 or Ser125 acts as an on/off switch for the activity of 5-hydroxyconiferaldehyde O-methyltransferase 2 (PtrAldOMT2), an enzyme central to monolignol biosynthesis for lignification in stem-differentiating xylem of Populus trichocarpa.
(via Semantic Scholar)
Sources: Web Of Science, ORCID, Crossref, NC State University Libraries
Added: August 6, 2018
2014 journal article
A robust chromatin immunoprecipitation protocol for studying transcription factor-DNA interactions and histone modifications in wood-forming tissue
NATURE PROTOCOLS, 9(9), 2180–2193.
MeSH headings : Base Sequence; Chromatin Immunoprecipitation / methods; DNA / metabolism; DNA Fragmentation; Histones / metabolism; Immunoprecipitation; Molecular Sequence Data; Populus / cytology; Populus / metabolism; Sequence Analysis, DNA; Transcription Factors / metabolism; Wood / cytology; Xylem / genetics; Xylem / metabolism
TL;DR:
This protocol is the only working ChIP system for wood-forming tissue and uses stem-differentiating xylem to identify genome-wide specific TF-DNA interactions during wood formation and histone modifications associated with regulation of wood formation.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger
(OpenAlex)
Source: Web Of Science
Added: August 6, 2018
2014 journal article
A simple improved-throughput xylem protoplast system for studying wood formation
NATURE PROTOCOLS, 9(9), 2194–2205.
MeSH headings : Cell Wall / metabolism; Gene Expression Regulation, Plant / genetics; Gene Regulatory Networks / genetics; Green Fluorescent Proteins; Populus / cytology; Populus / growth & development; Protoplasts / metabolism; Transfection / methods; Wood / cytology; Wood / growth & development; Xylem / chemistry
TL;DR:
A protocol for the isolation and transfection of protoplasts from wood-forming tissue, the stem-differentiating xylem (SDX), in the model woody plant Populus trichocarpa is presented.
(via Semantic Scholar)
Sources: Web Of Science, ORCID
Added: August 6, 2018
2014 journal article
Growth under field conditions affects lignin content and productivity in transgenic Populus trichocarpa with altered lignin biosynthesis
BIOMASS & BIOENERGY, 68, 228–239.
author keywords: Populus; Lignin; Field trial; Transgenic; Bioenergy
TL;DR:
The capacity to upregulate lignin in the event of environmental stress may have helped some low-lignin lines to survive and more research focused on promising construct lines in appropriate environmental conditions is needed to clarify if a significant reduction in lignIn can be achieved on a plantation scale, and whether that reduction will translate into increased efficiency of enzymatic hydrolysis.
(via Semantic Scholar)
Sources: Web Of Science, ORCID, NC State University Libraries
Added: August 6, 2018
2014 journal article
Systems Biology of Lignin Biosynthesis in Populus trichocarpa: Heteromeric 4-Coumaric Acid:Coenzyme A Ligase Protein Complex Formation, Regulation, and Numerical Modeling
The Plant Cell, 26(3), 876–893.
Contributors: H. Chen n, J. Song n, J. Wang n , Y. Lin n, J. Ducoste n , C. Shuford n, J. Liu n, Q. Li n
MeSH headings : Coenzyme A Ligases / genetics; Coenzyme A Ligases / metabolism; Coumaric Acids / metabolism; Immunoprecipitation; Lignin / biosynthesis; Mass Spectrometry; Models, Biological; Populus / metabolism; Propionates; RNA, Messenger / genetics; Substrate Specificity; Systems Biology
TL;DR:
This work shows that 4CL, an enzyme in monolignol biosynthesis, is found as a heterotetrameric complex of two isoforms in Populus trichocarpa, where the two 4-coumaric acid:CoA ligase (4CL) isoforms, 4CL3 and 4CL5, interact in vivo and in vitro to form a heterosymmetric protein complex.
(via Semantic Scholar)
Sources: Web Of Science, ORCID, NC State University Libraries, Crossref
Added: August 6, 2018
2014 journal article
Vibrational sum-frequency-generation (SFG) spectroscopy study of the structural assembly of cellulose microfibrils in reaction woods
CELLULOSE, 21(4), 2219–2231.
author keywords: Reaction wood; Tension wood; Compression wood; Cellulose microfibril assembly; Sum; frequency; generation spectroscopy; X-ray; diffraction
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018
2013 journal article
Monolignol Pathway 4-Coumaric Acid: Coenzyme A Ligases in Populus trichocarpa: Novel Specificity, Metabolic Regulation, and Simulation of Coenzyme A Ligation Fluxes
PLANT PHYSIOLOGY, 161(3), 1501–1516.
Contributors: H. Chen n, J. Song n, C. Williams n , C. Shuford n, J. Liu n, J. Wang n , Q. Li n, n
MeSH headings : Allosteric Regulation / drug effects; Binding Sites; Biosynthetic Pathways / drug effects; Blotting, Western; Caffeic Acids / pharmacology; Coenzyme A / metabolism; Coenzyme A Ligases / antagonists & inhibitors; Coenzyme A Ligases / metabolism; Computer Simulation; Coumaric Acids / chemistry; Coumaric Acids / metabolism; Coumaric Acids / pharmacology; Kinetics; Lignin / biosynthesis; Lignin / chemistry; Phenylpropionates / metabolism; Phosphoproteins / metabolism; Phosphorylation / drug effects; Plant Extracts; Populus / drug effects; Populus / enzymology; Propionates; Proteomics; Recombinant Fusion Proteins / metabolism; Sequence Homology, Amino Acid; Substrate Specificity / drug effects; Xylem / drug effects; Xylem / metabolism
TL;DR:
Evidence is presented of two monolignol 4CLs, Ptr4CL3 and P tr4CL5, in Populus trichocarpa that act to mitigate the inhibition by caffeic acid to maintain a proper ligation flux and coenzyme A flux with novel specificity.
(via Semantic Scholar)
Sources: Web Of Science, ORCID, NC State University Libraries
Added: August 6, 2018
2013 journal article
Regulation of phenylalanine ammonia-lyase (PAL) gene family in wood forming tissue of Populus trichocarpa
Planta, 238(3), 487–497.
Contributors: n, C. Shuford n, J. Wang n , Y. Sun n, Z. Yang n, H. Chen n, S. Tunlaya-Anukit n, Q. Li n
MeSH headings : Gene Expression Regulation, Plant / physiology; Mass Spectrometry; Phenylalanine Ammonia-Lyase / metabolism; Populus / enzymology; Populus / metabolism
TL;DR:
A greater functional significance of subgroup A PtrPALs for stem wood formation is indicated, and the role of gene post-transcriptional regulation is highlighted.
(via Semantic Scholar)
Sources: NC State University Libraries, ORCID, NC State University Libraries
Added: August 6, 2018
2013 journal article
The elucidation of the lignin structure effect on the cellulase-mediated saccharification by genetic engineering poplars (Populus nigra L. x Populus maximowiczii A.)
BIOMASS & BIOENERGY, 58, 52–57.
author keywords: Transgenic hybrid poplar; Pretreatment; S to V Ratio; Delignification; Enzymatic saccharification
TL;DR:
The decrease of the lignin and the hemicelluloses benefited the enzymatic saccharification with or without the pretreatment, and the high S to V ratio retarded the enzyme.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
7. Affordable and Clean Energy
(Web of Science)
12. Responsible Consumption and Production
(Web of Science)
Source: Web Of Science
Added: August 6, 2018
2012 journal article
A standard reaction condition and a single HPLC separation system are sufficient for estimation of monolignol biosynthetic pathway enzyme activities
PLANTA, 236(3), 879–885.
author keywords: Monolignols; Enzyme activity; Populus trichocarpa; Pinus taeda; Angiosperm; Gymnosperm
MeSH headings : Biosynthetic Pathways; Chromatography, High Pressure Liquid; Enzyme Assays; Lignin / biosynthesis; Methyltransferases / metabolism; Mixed Function Oxygenases / metabolism; Oxidoreductases / metabolism; Pinus taeda / enzymology; Plant Proteins / analysis; Plant Stems / enzymology; Populus / enzymology; Substrate Specificity; Xylem / enzymology
TL;DR:
This work developed a method using a total protein extract (soluble and microsomal) for the comprehensive and simultaneous analysis of these ten enzyme activities in a single target tissue, stem differentiating xylem (SDX) of Populustrichocarpa.
(via Semantic Scholar)
Source: Web Of Science
Added: August 6, 2018
2012 journal article
Comprehensive Quantification of Monolignol-Pathway Enzymes in Populus trichocarpa by Protein Cleavage Isotope Dilution Mass Spectrometry
Journal of Proteome Research, 11(6), 3390–3404.
Contributors: C. Shuford n, Q. Li n, Y. Sun n, H. Chen n, J. Wang n , n, R. Sederoff n, V. Chiang n, D. Muddiman n
author keywords: quantification; isotope dilution mass spectrometry; selected reaction monitoring; filter-aided sample preparation; monolignol biosynthesis
MeSH headings : Amino Acid Sequence; Biosynthetic Pathways; Isotope Labeling; Molecular Sequence Data; Plant Proteins / chemistry; Plant Proteins / metabolism; Populus / enzymology; Proteome / chemistry; Proteome / metabolism; Reference Standards; Spectrometry, Mass, Electrospray Ionization / standards; Xylem / enzymology
TL;DR:
This represents the most comprehensive assay for quantification of proteins regulating monolignol biosynthesis and will lead to a better understanding of lignin formation at a systems level.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
15. Life on Land
(OpenAlex)
Sources: Web Of Science, ORCID, Crossref, NC State University Libraries
Added: August 6, 2018
2012 journal article
MicroRNAs in trees
PLANT MOLECULAR BIOLOGY, 80(1), 37–53.
author keywords: MicroRNA; Woody plant; Gene regulation; Plant development; Plant stress response; Artificial microRNA
MeSH headings : Gene Expression Regulation, Plant; MicroRNAs / genetics; MicroRNAs / metabolism; Models, Genetic; Phylogeny; Plants, Genetically Modified / genetics; Plants, Genetically Modified / metabolism; RNA, Messenger / genetics; RNA, Messenger / metabolism; RNA, Plant / genetics; RNA, Plant / metabolism; Trees / classification; Trees / genetics; Trees / metabolism
TL;DR:
The purpose of this review is to summarize the current status of information on mi RNAs in trees and to guide future studies on the role of miRNAs in the biology of woody perennials and to illustrate their utility in directed genetic modification of trees.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger
(Web of Science)
13. Climate Action
(Web of Science)
Source: Web Of Science
Added: August 6, 2018
2010 journal article
Specific down-regulation of PAL genes by artificial microRNAs in Populus trichocarpa
PLANTA, 232(6), 1281–1288.
author keywords: Artificial microRNA; Phenylalanine ammonia-lyase; Populus trichocarpa transformation; Down-regulation; PolyA tailing-based real time RT-PCR; Quantitation
MeSH headings : Base Sequence; DNA Primers; Down-Regulation; Genes, Plant; MicroRNAs / physiology; Mutation; Populus / genetics; Reverse Transcriptase Polymerase Chain Reaction; Sequence Homology, Nucleic Acid
TL;DR:
This paper describes the specific down-regulation of multiple genes in the phenylalanine ammonia-lyase (PAL) gene family of Populus trichocarpa using amiRNA sequences incorporated in a ptc-MIR408 miRNA-producing precursor, revealing differential regulation within the gene family.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
15. Life on Land
(OpenAlex)
Source: Web Of Science
Added: August 6, 2018
2010 review
Towards a Systems Approach for Lignin Biosynthesis in Populus trichocarpa: Transcript Abundance and Specificity of the Monolignol Biosynthetic Genes
[Review of ]. PLANT AND CELL PHYSIOLOGY, 51(1), 144–163.
author keywords: Lignin systems biology; Monolignol biosynthesis; Populus trichocarpa; Promoter motifs; Transcript abundance; Xylem-specific expression
MeSH headings : Amino Acid Motifs / genetics; Biosynthetic Pathways / genetics; Enzymes / biosynthesis; Enzymes / genetics; Gene Expression Regulation, Enzymologic / physiology; Gene Expression Regulation, Plant / physiology; Genome, Plant / genetics; Lignin / biosynthesis; Lignin / genetics; Phloem / enzymology; Phloem / genetics; Plant Shoots / enzymology; Plant Shoots / genetics; Populus / genetics; Populus / metabolism; Promoter Regions, Genetic / genetics; RNA, Messenger / analysis; RNA, Messenger / genetics; RNA, Messenger / metabolism; RNA, Plant / analysis; RNA, Plant / genetics; RNA, Plant / metabolism; Transcription, Genetic / physiology; Xylem / enzymology; Xylem / genetics
TL;DR:
The identified 23 genes that most probably encode monolignol biosynthesis enzymes during wood formation are identified and evidence suggesting functional redundancy at the transcript level for phenylalanine ammonia-lyase and cinnamate 4-hydroxylase is found.
(via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018
2009 journal article
Isolation of expressed sequences from a specific chromosome of Thinopyrum intermedium infected by BYDV
GENOME, 52(1), 68–76.
author keywords: TAi-27; Thinopyrum intermedium; chromosome microdissection; hybridization-specific amplification (HSA); expressed sequences; barley yellow dwarf virus (BYDV)
MeSH headings : Chromosome Mapping; Chromosomes / ultrastructure; Chromosomes, Plant; Cloning, Molecular; DNA, Complementary / metabolism; DNA, Plant / metabolism; Expressed Sequence Tags; Gene Expression Regulation; Genes, Plant; Genome, Plant; Luteovirus / genetics; Models, Genetic; Oligonucleotides / genetics; RNA, Messenger / metabolism; Triticum / genetics; Triticum / virology
TL;DR:
A modified technique based on chromosome microdissection and hybridization-specific amplification to isolate expressed sequences from the alien chromosome of TAi-27 by hybridization between the DNA of the microdissected alien chromosome and cDNA of Th.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger
(Web of Science)
13. Climate Action
(Web of Science)
15. Life on Land
(Web of Science)
Source: Web Of Science
Added: August 6, 2018
2008 journal article
Rapid EST isolation from chromosome IR of rye
BMC PLANT BIOLOGY, 8.
MeSH headings : Chromosomes, Plant / genetics; DNA, Complementary / genetics; DNA, Plant / genetics; Expressed Sequence Tags; Immunoblotting; Secale / genetics
TL;DR:
Isolated in this research were 22 ESTs with unknown functions, probably representing some new genes on rye chromosome 1R, by combining the techniques of chromosome microdissection with hybrid specific amplification (HSA).
(via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger
(Web of Science)
13. Climate Action
(Web of Science)
15. Life on Land
(Web of Science)
Source: Web Of Science
Added: August 6, 2018
2005 journal article
Facile means for quantifying microRNA expression by real-time PCR
BIOTECHNIQUES, 39(4), 519–525.
MeSH headings : Arabidopsis / genetics; Base Sequence; Benzothiazoles; Blotting, Northern; DNA Primers / chemistry; DNA, Complementary / metabolism; Diamines; Fluorescent Dyes / pharmacology; Genes, Plant / genetics; MicroRNAs / analysis; Molecular Sequence Data; Organic Chemicals / pharmacology; Plant Leaves / metabolism; Plant Stems / metabolism; Polymerase Chain Reaction; Quinolines; RNA / metabolism; Reverse Transcriptase Polymerase Chain Reaction / methods; Sensitivity and Specificity; Temperature; Tissue Distribution; Transcription, Genetic
TL;DR:
A simple and sensitive real-time PCR method for quantifying the expression of plant miRNAs and revealed miRNA tissue-specific expression patterns that cannot be resolved by Northern blot analysis and may therefore be widely useful for characterizing miRNA expression in plants as well as in animals.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
10. Reduced Inequalities
(OpenAlex)
Source: Web Of Science
Added: August 6, 2018
2005 journal article
Novel and mechanical stress-responsive microRNAs in Populus trichocarpa that are absent from Arabidopsis
Plant Cell, 17(8), 2186–2203.
MeSH headings : Arabidopsis / genetics; Base Sequence; Conserved Sequence; Gene Expression Regulation, Plant; MicroRNAs / chemistry; MicroRNAs / genetics; Molecular Sequence Data; Plant Stems / genetics; Populus / genetics; RNA, Plant / genetics; Species Specificity; Transcription, Genetic
TL;DR:
It is shown that plant miRNAs can be induced by mechanical stress and may function in one of the most critical defense systems for structural and mechanical fitness.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
Source: NC State University Libraries
Added: August 6, 2018
2004 journal article
RNA silencing in plants by the expression of siRNA duplexes
NUCLEIC ACIDS RESEARCH, 32(21).
MeSH headings : Gene Expression Regulation, Plant; Genetic Vectors; Glucuronidase / analysis; Glucuronidase / genetics; Humans; Molecular Sequence Data; Promoter Regions, Genetic; RNA / genetics; RNA Interference; RNA, Small Cytoplasmic / genetics; RNA, Small Interfering / biosynthesis; RNA, Small Interfering / chemistry; RNA, Small Interfering / genetics; Signal Recognition Particle / genetics; Tobacco / genetics; Tobacco / metabolism
TL;DR:
It is reported that RNA silencing was achieved in plants through stable expression of short hairpin RNA, which was driven by Pol III RNA gene promoters, and the effectiveness of the human H1 promoter in a plant background suggested a conserved mechanism underlying Pol III complex functionality.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science; OpenAlex)
Source: Web Of Science
Added: August 6, 2018
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