Ramsey S Lewis

Works (78)

Updated: December 12th, 2024 05:07

2024 article

A 2-year, multi-county survey of plant-parasitic nematodes in North Carolina flue-cured tobacco

Bonyak, H. C., Vann, M. C., Ye, W., Lewis, R. S., & Gorny, A. M. (2024, March 27). AGRONOMY JOURNAL, Vol. 3.

By: H. Bonyak n, M. Vann n , W. Ye*, R. Lewis n  & A. Gorny n

TL;DR: The previous crop had a significant effect on root‐knot and spiral nematodes in 2021, and lesion and stubby root nematodes in 2022, and tobacco variety did not have an effect on nematode population density. (via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger (Web of Science)
3. Good Health and Well-being (OpenAlex)
13. Climate Action (Web of Science)
Sources: Web Of Science, NC State University Libraries, ORCID
Added: April 1, 2024

2024 article

A Comparison of Three Automated Root-Knot Nematode Egg Counting Approaches Using Machine Learning, Image Analysis, and a Hybrid Model

Fraher, S. P., Watson, M., Nguyen, H., Moore, S., Lewis, R. S., Kudenov, M., … Gorny, A. M. (2024, September 9). PLANT DISEASE, Vol. 9.

By: S. Fraher n, M. Watson *, H. Nguyen n, S. Moore n, R. Lewis n , M. Kudenov n, G. Yencho  n, A. Gorny n

author keywords: automated counting; convolutional neural network; meloidogyne; root-knot nematode
Sources: Web Of Science, NC State University Libraries
Added: September 23, 2024

2024 article

Engineering Sclareol Production on the Leaf Surface of <i>Nicotiana tabacum</i>

Ma, H., Steede, T., Dewey, R. E., & Lewis, R. S. (2024, June 6). JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, Vol. 6.

By: H. Ma n, T. Steede n, R. Dewey n & R. Lewis n 

author keywords: sclareol; terpene; terpenoid; metabolicengineering; tobacco; trichome; diterpenoid; leaf surface; secondary metabolite
UN Sustainable Development Goal Categories
Sources: Web Of Science, NC State University Libraries
Added: June 24, 2024

2023 article

Analysis of alkaloids and reducing sugars in processed and unprocessed tobacco leaves using a handheld near infrared spectrometer

Castillo, M. S., Acosta, J. J., Hodge, G. R., Vann, M. C., & Lewis, R. S. (2023, January 16). JOURNAL OF NEAR INFRARED SPECTROSCOPY, Vol. 1.

By: M. Castillo n , J. Acosta  n, G. Hodge n, M. Vann n  & R. Lewis n 

author keywords: Near infrared spectroscopy; handheld; alkaloids; tobacco; reducing sugars; unprocessed; intact
UN Sustainable Development Goal Categories
2. Zero Hunger (Web of Science)
3. Good Health and Well-being (OpenAlex)
Sources: Web Of Science, NC State University Libraries
Added: February 13, 2023

2023 journal article

Effects of Recurrent Selection on Population Structure and Allele Frequencies in the M3S Maize Population

AGRICULTURE-BASEL, 14(1).

By: M. Bukan*, A. Mandic *, V. Kozumplik*, R. Lewis n , D. Simic *, H. Sarcevic *, Y. Liu

author keywords: maize; recurrent selection; grain yield; allele frequency; population structure; SSR markers
UN Sustainable Development Goal Categories
13. Climate Action (Web of Science)
15. Life on Land (Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: February 12, 2024

2023 journal article

Modified physiology of burley tobacco plants genetically engineered to express Yb<sub>1</sub>, a functional EGY enzyme

PLANTA, 258(4).

By: R. Shi n, S. Kernodle n, T. Steede n & R. Lewis n 

author keywords: Burley tobacco; Gene editing; Nicotiana tabacum; Nitrogen use efficiency; RNA-sequencing
TL;DR: Results indicate that complex changes in the transcriptome of tobacco can be manifested by altered expression of Yb 1, and metabolic pathways affecting carbon and nitrogen metabolism that might be altered as the result of genetic variability at the Yb1 and Yb2 loci. (via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger (Web of Science)
3. Good Health and Well-being (OpenAlex)
Sources: Web Of Science, NC State University Libraries
Added: October 23, 2023

2023 article

Use of exotic Nicotiana tabacum germplasm for confronting an inverse genetic correlation in flue-cured tobacco

Lewis, R. S. S. (2023, April 14). CROP SCIENCE, Vol. 4.

By: R. Lewis n 

UN Sustainable Development Goal Categories
2. Zero Hunger (Web of Science)
3. Good Health and Well-being (OpenAlex)
15. Life on Land (Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: May 9, 2023

2022 article

Adding genome-wide genotypic information to a tobacco (Nicotiana tabacum) breeding programme

Carvalho, B. L., Lewis, R., Bruzi, A. T., Padua, J. M. V., & Patto Ramalho, M. A. (2022, January 21). PLANT BREEDING, Vol. 1.

By: B. Carvalho, R. Lewis n , A. Bruzi *, J. Padua * & M. Patto Ramalho *

author keywords: breeding; diallel; genome wide selection; hybrid; prediction; tobacco
UN Sustainable Development Goal Categories
3. Good Health and Well-being (OpenAlex)
13. Climate Action (Web of Science)
15. Life on Land (Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: January 31, 2022

2022 journal article

Analyses of diverse low alkaloid tobacco germplasm identify naturally occurring nucleotide variability contributing to reduced leaf nicotine accumulation

MOLECULAR BREEDING, 42(1).

By: N. Burner  n, A. McCauley n, S. Pramod *, J. Frederick *, T. Steede n, S. Kernodle n, R. Lewis n 

author keywords: Nicotiana tabacum; Tobacco; Nicotine alkaloids; Transcription factors; QTL mapping
TL;DR: P phenotyped a series of 81 selected diverse tobacco introductions to identify a sub-group with authentic low alkaloid phenotypes and found a newly discovered 5 bp deletion in the gene MYC2a on linkage group 5 was found to likely partially underlie the ultra-low alkaloids phenotype of TI 313. (via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger (Web of Science)
3. Good Health and Well-being (OpenAlex)
13. Climate Action (Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: January 24, 2022

2022 journal article

CLCNt2 Mediates Nitrate Content in Tobacco Leaf, Impacting the Production of Tobacco-Specific Nitrosamines in Cured Leaves

FRONTIERS IN PLANT SCIENCE, 13.

By: L. Bovet*, P. Campanoni*, J. Lu  n, A. Hilfiker*, S. Kleinhans*, H. Laparra*, J. Schwaar*, R. Lewis n  ...

author keywords: CLCNt2; chloride channel; tobacco; NNN; NNK; nitrate transport; chloride; proton antiporters; tobacco-specific nitrosamines
TL;DR: This study provides a straight path toward the development of new commercial tobacco varieties with reduced TSNA levels by breeding of variants deficient in active CLCNt2 copies byressing expression or knocking-out CLCnt2 has a direct impact on leaf nitrate and TSNA reduction in cured leaves without altering biomass. (via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger (Web of Science)
3. Good Health and Well-being (OpenAlex)
Sources: Web Of Science, NC State University Libraries
Added: March 14, 2022

2022 article

Combined reduced expression of two gene families lowers nicotine content to ultra-low levels in cultivated tobacco

Kernodle, S. P., Webb, S., Steede, T. M., & Lewis, R. S. (2022, July 2). PLANT CELL REPORTS, Vol. 7.

By: S. Kernodle n, S. Webb n, T. Steede n & R. Lewis n 

author keywords: Tobacco; Nicotiana tabacum; Nicotine; Alkaloids; RNA interference; MPO; Methyl Putrescine Oxidase
MeSH headings : Gene Expression Regulation, Plant; Plant Leaves / genetics; Plant Leaves / metabolism; RNA Interference; Tobacco / genetics; Tobacco / metabolism; Tobacco Products
TL;DR: Tobacco genotypes expressing an RNAi construct designed to reduce expression of the Methyl Putrescine Oxidase (MPO) gene family associated with nicotine biosynthesis are produced and evaluated under field conditions. (via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger (Web of Science)
13. Climate Action (Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: July 11, 2022

2022 journal article

Editing of A622 genes results in ultra-low nicotine whole tobacco plants at the expense of dramatically reduced growth and development

MOLECULAR BREEDING, 42(4).

By: N. Burner  n, S. Kernodle n, T. Steede n & R. Lewis n 

author keywords: Tobacco; Nicotiana tabacum; Nicotine; Alkaloids; Gene editing; A622
TL;DR: A622 knockout lines exhibited lower nicotine levels than previously created genotypes with deleterious mutations in BBL genes also associated with one of the latter steps in tobacco alkaloid biosynthesis. (via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger (Web of Science)
3. Good Health and Well-being (OpenAlex)
13. Climate Action (Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: April 18, 2022

2022 article

Editorial: Biotechnological and genomic approaches for enhancing agronomic performance of crops

Mir, R. R., Kudapa, H., Pramod, S., & Lewis, R. S. (2022, October 4). FRONTIERS IN GENETICS, Vol. 13.

By: R. Mir *, H. Kudapa *, S. Pramod * & R. Lewis n 

author keywords: biotechnology and bioinformatics; genomics (genetic tests and databases); crop improvement; gene; markers
TL;DR: Investigations provide the gene expression changes mediated by abiotic stresses in transgenic soybean overexpressing AtARA6 gene, and some insights into the mechanism mediating salt tolerance in soybean. (via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger (OpenAlex)
13. Climate Action (Web of Science)
15. Life on Land (Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: December 12, 2022

2022 journal article

Exploitation of heterosis in tobacco breeding in Brazil

CROP BREEDING AND APPLIED BIOTECHNOLOGY, 22(2).

By: B. Carvalho, A. Bruzi *, R. Lewis n , J. Villela Padua * & M. Patto Ramalho *

author keywords: Plant breeding; quantitative genetics; diallel crosses; hybrid vigor
UN Sustainable Development Goal Categories
3. Good Health and Well-being (OpenAlex)
13. Climate Action (Web of Science)
15. Life on Land (Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: July 26, 2022

2021 journal article

Adaptation of Phytophthora nicotianae to Multiple Sources of Partial Resistance in Tobacco

PLANT DISEASE, 106(3), 906–917.

By: J. Jin n, K. McCorkle n, V. Cornish n , I. Carbone n , R. Lewis n  & H. Shew  n

author keywords: adaptation; ddRADseq; host resistance; Nicotiana rustica; Phytophthora nicotianae; tobacco
MeSH headings : Genotype; Phytophthora / genetics; Plant Diseases / genetics; Quantitative Trait Loci / genetics; Tobacco / genetics
TL;DR: The rapid adaptation to Wz resistance and the gradual adaptation to other QTLs highlights the need to study the nature of WZ resistance and for conducting field studies on efficacy of resistance-gene rotation for disease management. (via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being (OpenAlex)
Sources: Web Of Science, NC State University Libraries
Added: July 26, 2022

2021 journal article

Combining ability of standardized indices for multi-trait selection in tobacco

CIENCIA E AGROTECNOLOGIA, 45.

By: B. Carvalho *, R. Lewis n , J. Villela Padua *, A. Bruzi * & M. Patto Ramalho *

author keywords: Plant breeding; quantitative genetics; heterosis
UN Sustainable Development Goal Categories
3. Good Health and Well-being (OpenAlex)
13. Climate Action (Web of Science)
15. Life on Land (Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: November 15, 2021

2021 journal article

Constitutive activation of nitrate reductase in tobacco alters flowering time and plant biomass

SCIENTIFIC REPORTS, 11(1).

By: J. Lu n, N. Chandrakanth  n, R. Lewis n , K. Andres n, L. Bovet*, S. Goepfert *, R. Dewey n

MeSH headings : Biomass; Environment; Flowers / genetics; Gene Expression Regulation, Plant; Gene-Environment Interaction; Genetic Association Studies; Nitrate Reductase / genetics; Nitrate Reductase / metabolism; Organ Specificity / genetics; Plant Development / genetics; Tobacco / physiology; Transcriptional Activation
TL;DR: It is shown that plants expressing a constitutively active NR construct, designated 35S:S523D-NR, display an early-flowering phenotype that is also associated with a substantial reduction in plant biomass, and the mechanism by which yield was restored differed markedly between the two crosses. (via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger (Web of Science)
3. Good Health and Well-being (OpenAlex)
Sources: Web Of Science, NC State University Libraries
Added: March 22, 2021

2021 journal article

Evaluation of tobacco lines and hybrids carrying Beinhart-1000 alleles at the Phn15.1 locus for agronomic and disease resistance characteristics

CROP SCIENCE, 61(4), 2456–2466.

By: C. Heim n, J. Ma n, E. Willard*, C. Shelton * & R. Lewis n 

UN Sustainable Development Goal Categories
3. Good Health and Well-being (OpenAlex)
Sources: Web Of Science, NC State University Libraries
Added: May 24, 2021

2021 article

Homoeologous chromosome exchange explains the creation of a QTL affecting soil-borne pathogen resistance in tobacco

Shi, R., Jin, J., Nifong, J. M., Shew, D., & Lewis, R. S. (2021, October 22). PLANT BIOTECHNOLOGY JOURNAL, Vol. 10.

By: R. Shi n, J. Jin n, J. Nifong n, D. Shew n & R. Lewis n 

author keywords: homoeologous exchange; QTL; resistance; SAR8; 2; tobacco; Phytophthora
MeSH headings : Chromosomes, Plant / genetics; Disease Resistance / genetics; Plant Diseases / genetics; Quantitative Trait Loci / genetics; Soil; Tobacco / genetics
TL;DR: A role for genomic rearrangements in the generation of favourable genetic variability affecting resistance to pathogens in plants is suggested. (via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger (Web of Science)
3. Good Health and Well-being (OpenAlex)
13. Climate Action (Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: November 1, 2021

2021 journal article

QTL analysis of seed germination traits in tobacco (Nicotiana tabacum L.)

JOURNAL OF APPLIED GENETICS, 62(3), 441–444.

By: M. Agacka-Moldoch *, M. Rehman Arif *, U. Lohwasser *, T. Doroszewska *, R. Lewis n  & A. Boerner

author keywords: Seeds; Germination; Controlled deterioration; Storability; Genetic mapping
MeSH headings : Chromosome Mapping; Germination; Quantitative Trait Loci; Seeds / genetics; Seeds / physiology; Tobacco / genetics
TL;DR: A bi-parental mapping population consisting of 118 doubled haploid lines and derived from a cross between ‘Beinhart-1000’ and ‘Hicks’ was investigated and demonstrated that germination-related traits are very complex and under polygenic control. (via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger (Web of Science)
3. Good Health and Well-being (OpenAlex)
13. Climate Action (Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: March 29, 2021

2021 journal article

RNAseq Reveals Differential Gene Expression Contributing to Phytophthora nicotianae Adaptation to Partial Resistance in Tobacco

AGRONOMY-BASEL, 11(4).

By: J. Jin n, R. Shi n, R. Lewis n  & H. Shew n

author keywords: RNAseq; Phytophthora nicotianae; adaptation; partial resistance; tobacco
TL;DR: Understanding of the molecular mechanisms of P. nicotianae adaptation to partial resistance in tobacco is enhanced by elucidating how the pathogen recruits pathogenicity-associated genes that impact host biological activities. (via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger (Web of Science)
3. Good Health and Well-being (OpenAlex)
13. Climate Action (Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: May 10, 2021

2020 journal article

Characterization of Phn15.1, a Newly Identified Phytophthora nicotianae Resistance QTL in Nicotiana tabacum

PLANT DISEASE, 104(6), 1638–1646.

By: J. Ma*, C. Heim n, M. Humphry, J. Nifong n & R. Lewis n 

author keywords: black shank; Nicotiana; partial resistance; Phytophthora; plant disease resistance; quantitative trait locus; tobacco
MeSH headings : Alleles; Phytophthora; Plant Diseases; Quantitative Trait Loci; Tobacco
UN Sustainable Development Goal Categories
3. Good Health and Well-being (OpenAlex)
Sources: Web Of Science, NC State University Libraries
Added: June 15, 2020

2020 journal article

Genetic and Agronomic Analysis of Tobacco Genotypes Exhibiting Reduced Nicotine Accumulation Due to Induced Mutations in Berberine Bridge Like (BBL) Genes

FRONTIERS IN PLANT SCIENCE, 11.

By: R. Lewis n , K. Drake-Stowe n, C. Heim n, T. Steede n, W. Smith n & R. Dewey n

author keywords: tobacco; Nicotiana tabacum; nicotine; alkaloids; gene editing; regulation
TL;DR: Genetic methodologies for reducing nicotine accumulation in the tobacco plant (Nicotiana tabacum L.) are of interest because of potential future regulations that could mandate lowering of this alkaloid in conventional cigarettes. (via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger (Web of Science)
3. Good Health and Well-being (OpenAlex)
13. Climate Action (Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: May 18, 2020

2020 journal article

Genetics influence postharvest measurements of flue-cured tobacco more than nitrogen application rate

AGRONOMY JOURNAL, 113(2), 1020–1028.

By: J. Cheek n, M. Vann n , R. Lewis n  & L. Fisher n

UN Sustainable Development Goal Categories
3. Good Health and Well-being (Web of Science; OpenAlex)
Sources: Web Of Science, NC State University Libraries, ORCID
Added: December 21, 2020

2020 journal article

Identification and editing of a hybrid lethality gene expands the range of interspecific hybridization potential in Nicotiana

THEORETICAL AND APPLIED GENETICS, 133(10), 2915–2925.

By: J. Ma n, W. Hancock n, J. Nifong n, S. Kernodle n & R. Lewis n 

MeSH headings : CRISPR-Cas Systems; Crosses, Genetic; DNA Transposable Elements; Gene Editing; Genes, Lethal; Genes, Plant; Hybridization, Genetic; Tobacco / genetics
TL;DR: Gene editing was used to verify that Nt6549g30 is the gene at the N. tabacum Hybrid Lethality 1 (NtHL1) locus controlling the hybrid lethality reaction in crosses with N. africana and demonstrate the possibility of expanding the gene pool for a crop species through gene editing. (via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger (Web of Science)
13. Climate Action (Web of Science)
15. Life on Land (OpenAlex)
Sources: Web Of Science, NC State University Libraries
Added: July 20, 2020

2019 review

Agronomic Practices Affecting Nicotine Concentration in Flue-Cured Tobacco: A Review

[Review of ]. AGRONOMY JOURNAL, 111(6), 3067–3075.

By: J. Henry  n, M. Vann n  & R. Lewis n 

TL;DR: This comprehensive review discusses the agronomic factors affecting alkaloid production in flue-cured tobacco, and how these factors can be adjusted to manipulate the ultimate nicotine concentration. (via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger (OpenAlex)
Sources: Web Of Science, NC State University Libraries
Added: January 21, 2020

2019 journal article

Evaluating diverse systems of tobacco genetic resistance to Phytophthora nicotianae in Yunnan, China

PLANT PATHOLOGY, 68(9), 1616–1623.

By: J. Zeng *, J. Nifong n, Y. Liu *, C. Huang *, D. Fang*, R. Lewis n , Y. Li *

author keywords: black shank; resistance; tobacco
TL;DR: Findings suggest that both Wz− and Beinhart 1000‐mediated resistance have important commercial value in flue‐cured tobacco breeding programmes in China and Cultivars developed for black shank resistance in China may also have utility in other tobacco‐growing areas. (via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being (OpenAlex)
Sources: Web Of Science, NC State University Libraries
Added: November 25, 2019

2019 journal article

Genetic Control of Facultative Parthenocarpy in Nicotiana tabacum L.

JOURNAL OF HEREDITY, 110(5), 610–617.

By: A. Dexter-Boone n, M. Humphry*, R. Shi n & R. Lewis n 

author keywords: Genomics and gene mapping; haploids; Nicotiana; parthenocarpy; QTL mapping; tobacco
MeSH headings : Chromosome Mapping; Fruit / genetics; Genetic Association Studies; Haploidy; Parthenogenesis / genetics; Quantitative Trait Loci; Quantitative Trait, Heritable; Seeds / genetics; Tobacco / genetics
TL;DR: Investigation of parthenocarpy, the production of fruit without fertilization, in multiple plant species could result in development of technologies for conferring seedless fruits and increased stability of fruit formation in economically important plants. (via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger (Web of Science)
3. Good Health and Well-being (OpenAlex)
13. Climate Action (Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: September 16, 2019

2019 journal article

Genetic analysis of Phn7.1, a major QTL conferring partial resistance to Phytophthora nicotianae in Nicotiana tabacum

MOLECULAR BREEDING, 39(1).

By: J. Ma*, C. Heim n, M. Humphry, J. Nifong n & R. Lewis n 

TL;DR: Genotyping of historical materials with Phn7.1-associated SNP markers strongly suggests that the favorable Phn 7.1 allele(s) is present in most modern US flue-cured cultivars and absent in early predecessor germplasm. (via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being (OpenAlex)
Sources: NC State University Libraries, NC State University Libraries
Added: January 21, 2019

2019 journal article

Heterosis in Flue-Cured Tobacco and Its Utility in Predicting Transgressive Segregation within Derived Populations of Inbred Lines

CROP SCIENCE, 59(3), 957–967.

By: A. Dexter-Boone n & R. Lewis n 

UN Sustainable Development Goal Categories
3. Good Health and Well-being (OpenAlex)
13. Climate Action (Web of Science)
15. Life on Land (Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: June 4, 2019

2019 journal article

Identification and validation of SNP markers associated with Wz-mediated Phytophthora nicotianae resistance in Nicotiana tabacum L.

MOLECULAR BREEDING, 39(7).

By: R. Shi n, H. Hubert n, A. Dexter-Boone n, J. Zeng *, S. Kernodle n & R. Lewis n 

author keywords: Nicotiana; Tobacco; RNA-seq; Black shank; Phytophthora nicotianae
TL;DR: The utility of a subset of derived Kompetitive Allele Specific PCR (KASP) and Cleaved Amplified Polymorphic Sequence (CAPS) markers for predicting the presence of Wz was verified using segregating populations and F3:4 families that were evaluated for field black shank resistance. (via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being (OpenAlex)
Sources: Web Of Science, NC State University Libraries
Added: July 22, 2019

2019 review

Increasing Photosynthesis: Unlikely Solution For World Food Problem

[Review of ]. TRENDS IN PLANT SCIENCE, 24(11), 1032–1039.

By: T. Sinclair n, T. Rufty n & R. Lewis n 

MeSH headings : Carbon; Edible Grain; Nitrogen; Photosynthesis; Plant Leaves; Seeds
TL;DR: In this Opinion article, nitrogen limitation is given particular attention because of its quantitative linkage with vegetative and reproductive growth and its essential role as a quantitative component of seeds. (via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger (Web of Science; OpenAlex)
13. Climate Action (Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: November 18, 2019

2018 journal article

Filter paper-based spin column method for cost-efficient DNA or RNA purification

PLOS ONE, 13(12).

By: R. Shi n, R. Lewis n  & D. Panthee n 

MeSH headings : Centrifugation / methods; DNA, Plant / chemistry; DNA, Plant / isolation & purification; Solanum lycopersicum / chemistry; Paper; RNA, Plant / chemistry; RNA, Plant / isolation & purification; Tobacco / chemistry
UN Sustainable Development Goal Categories
Sources: Web Of Science, NC State University Libraries
Added: December 31, 2018

2018 article

Potential Mandated Lowering of Nicotine Levels in Cigarettes: A Plant Perspective

Lewis, R. S. (2019, July). NICOTINE & TOBACCO RESEARCH, Vol. 21, pp. 991–995.

By: R. Lewis n 

MeSH headings : Alkaloids / adverse effects; Alkaloids / genetics; Genetic Engineering / methods; Genetic Engineering / trends; Humans; Nicotine / adverse effects; Nicotine / genetics; Tobacco Products / adverse effects; United States; United States Food and Drug Administration / trends; World Health Organization
TL;DR: An understanding of technical feasibility of plant-based nicotine reduction technologies, along with the potential for corresponding alterations in alkaloid or toxicant profiles, is needed by regulators to develop effective nicotine control strategies with minimal impediments or undesirable consequences. (via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being (Web of Science; OpenAlex)
Sources: Web Of Science, NC State University Libraries
Added: November 18, 2019

2017 journal article

A reference genome for Nicotiana tabacum enables map-based cloning of homeologous loci implicated in nitrogen utilization efficiency

BMC GENOMICS, 18.

By: K. Edwards *, N. Fernandez-Pozo *, K. Drake-Stowe n, M. Humphry*, A. Evans*, A. Bombarely *, F. Allen*, R. Hurst * ...

author keywords: Sequencing; Nicotiana; Nicotiana tabacum; tobacco; Solanaceae; Nitrogen use efficiency; Nitrogen utilization efficiency; EGY1; Map-based cloning; Polyploidy
MeSH headings : Cloning, Molecular; Evolution, Molecular; Genetic Loci / genetics; Genome, Plant / genetics; Genomics / standards; Nitrogen / metabolism; Reference Standards; Tobacco / genetics; Tobacco / metabolism
TL;DR: An improved tobacco genome assembly is reported, which, aided by the application of optical mapping, achieves an N50 size and enables anchoring of 64% of the genome to pseudomolecules; a significant increase from the previous value of 19%. (via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger (Web of Science; OpenAlex)
13. Climate Action (Web of Science)
15. Life on Land (Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2017 journal article

Characterization of Phytophthora nicotianae Resistance Conferred by the Introgressed Nicotiana rustica Region, Wz, in Flue-Cured Tobacco

PLANT DISEASE, 102(2), 309–317.

By: K. McCorkle  n, K. Drake-Stowe n, R. Lewis n  & D. Shew n

MeSH headings : Genotype; Phytophthora / pathogenicity; Phytophthora / physiology; Plant Diseases / genetics; Plant Diseases / microbiology; Tobacco / genetics; Tobacco / microbiology; Virulence
TL;DR: This study found Wz-mediated resistance is most highly expressed in the roots, with only a slight reduction in stem-lesion size in Wz genotypes compared with susceptible controls, suggesting pathogen adaptation was specific to the Wz gene. (via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being (OpenAlex)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2017 journal article

Heterosis, transmission genetics, and selection for increased growth rate in a N. tabacum x synthetic tobacco cross

MOLECULAR BREEDING, 37(4).

By: W. Hancock n & R. Lewis n 

author keywords: Nicotiana; Tobacco; Synthetic amphidiploid; Yield; Growth rate; Genetic variability
TL;DR: Crosses between N. tabacum and synthetic tobaccos may provide superior routes for genome-wide introgression from diploid relatives and allow increased potential to capitalize on heterotic effects in tobacco, suggesting populations derived from N.tabacum × synthetic tobacco crosses may be more efficient for introgressing germplasm from diPloid relatives. (via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger (Web of Science)
13. Climate Action (Web of Science)
15. Life on Land (Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2017 journal article

Multiple Disease Resistance Loci Affect Soilborne Disease Resistance in Tobacco (Nicotiana tabacum)

PHYTOPATHOLOGY, 107(9), 1055–1061.

By: K. Drake-Stowe*, N. Bakaher*, S. Goepfert *, B. Philippon*, R. Mark*, P. Peterson*, R. Lewis* 

author keywords: bacterial wilt; black shank; Nicotiana
MeSH headings : DNA, Plant / genetics; Genetic Linkage; Genetic Markers; Genetic Predisposition to Disease; Genotype; Plant Diseases / genetics; Plant Diseases / microbiology; Quantitative Trait Loci; Soil Microbiology; Tobacco / genetics; Tobacco / microbiology
TL;DR: A quantitative trait loci mapping experiment designed to investigate the genetic control of soilborne disease resistance of highly resistant 'K346' tobacco identified four QTL significantly associated with resistance to P. nicotianae and three QTL to be associated with R. solanacearum resistance. (via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being (OpenAlex)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2017 journal article

The Tobacco Trichome Exudate Z-abienol and Its Relationship With Plant Resistance to Phytophthora nicotianae

PLANT DISEASE, 101(7), 1214–1221.

By: W. Steede n, J. Ma, D. Eickholt n, K. Drake-Stowe n, S. Kernodle n, H. Shew  n, D. Danehower n, R. Lewis n 

TL;DR: Results suggest that, although Z-abienol may affect P. nicotianae when applied at high concentrations in in vitro assays, the compound has little effect on black shank disease development under natural field conditions. (via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being (OpenAlex)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2016 journal article

Expression of a constitutively active nitrate reductase variant in tobacco reduces tobacco-specific nitrosamine accumulation in cured leaves and cigarette smoke

PLANT BIOTECHNOLOGY JOURNAL, 14(7), 1500–1510.

By: J. Lu n, L. Zhang n, R. Lewis n , L. Bovet*, S. Goepfert *, A. Jack*, J. Crutchfield*, H. Ji *, R. Dewey n

author keywords: nitrate reductase; tobacco; tobacco-specific nitrosamines; burley; nitrogen-assimilation pathway; NNN; NNK
MeSH headings : Carcinogens / metabolism; Metabolic Networks and Pathways; Nitrate Reductase / genetics; Nitrates / metabolism; Nitrogen / metabolism; Nitrosamines / metabolism; Plant Leaves / genetics; Plant Leaves / metabolism; Plants, Genetically Modified / metabolism; Tobacco / genetics; Tobacco / metabolism; Tobacco Products
TL;DR: Investigation of the potential of molecular genetic strategies to lower nitrate levels in burley tobaccos by overexpressing genes encoding key enzymes of the nitrogen‐assimilation pathway found only the expression of a constitutively active nitrate reductase (NR) dramatically decreased free nitrates levels in the leaves. (via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being (Web of Science; OpenAlex)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2016 journal article

Heat Unit Accumulation and Days to Anthesis Relationship in Tobacco Genotypes with an Introgressed QTL Affecting Leaf Number

CROP SCIENCE, 56(6), 3228–3236.

By: R. Wells n, D. Eickholt n, R. Lewis n , M. Vann n  & L. Fisher n

Contributors: R. Wells n, D. Eickholt n, R. Lewis n , M. Vann n  & L. Fisher n

UN Sustainable Development Goal Categories
2. Zero Hunger (Web of Science)
3. Good Health and Well-being (OpenAlex)
13. Climate Action (Web of Science)
Sources: Web Of Science, NC State University Libraries, ORCID
Added: August 6, 2018

2015 journal article

Genetic Factors for Enhancement of Nicotine Levels in Cultivated Tobacco

SCIENTIFIC REPORTS, 5.

By: B. Wang *, R. Lewis n , J. Shi *, Z. Song *, Y. Gao *, W. Li *, H. Chen n, R. Qu n

MeSH headings : Nicotine / biosynthesis; Nicotine / genetics; Plant Leaves / genetics; Plant Leaves / metabolism; Plant Proteins / genetics; Plant Proteins / metabolism; Plants, Genetically Modified / genetics; Plants, Genetically Modified / metabolism; Tobacco / genetics; Tobacco / metabolism; Transcription Factors / genetics; Transcription Factors / metabolism
TL;DR: It is demonstrated that the increased nicotine trait was stably inherited to the T2 and T3 generations, indicating the important role that Nt MYC2a plays in regulating nicotine accumulation in N. tabacum and the great potential of NtMYC 2a overexpression in tobacco plants for industrial nicotine production. (via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger (Web of Science)
3. Good Health and Well-being (OpenAlex)
13. Climate Action (Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2015 journal article

Identification of maternal haploids of Nicotiana tabacum aided by transgenic expression of green fluorescent protein: evidence for chromosome elimination in the N-tabacum x N-africana interspecific cross

MOLECULAR BREEDING, 35(9).

By: W. Hancock n, V. Kuraparthy n , S. Kernodle n & R. Lewis n 

author keywords: Nicotiana; Haploids; Hybrid lethality; Tobacco; Chromosome elimination; Parthenogenesis
TL;DR: The results present evidence of genetic instability in progeny from the N. africana cross and suggest that, in contrast to previous belief, chromosome elimination may play at least a partial role in the generation of haploids from this cross. (via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger (Web of Science)
3. Good Health and Well-being (OpenAlex)
13. Climate Action (Web of Science)
15. Life on Land (Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2015 journal article

Mapping quantitative trait loci determining seed longevity in tobacco (Nicotiana tabacum L.)

EUPHYTICA, 202(3), 479–486.

By: M. Agacka-Modoch, M. Nagel *, T. Doroszewska *, R. Lewis n  & A. Boerner

author keywords: Controlled deterioration; Germination; QTL mapping; Storability
TL;DR: 122 recombinant inbred lines derived from a cross between the cultivars Florida 301 and Hicks are investigated, finding that Florida 301 seems to withstand the CD treatment better, having increased seed longevity. (via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger (Web of Science)
13. Climate Action (Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2015 journal article

Transgenic and Mutation-Based Suppression of a Berberine Bridge Enzyme-Like (BBL) Gene Family Reduces Alkaloid Content in Field-Grown Tobacco

PLOS ONE, 10(2).

By: R. Lewis n , H. Lopez n, S. Bowen n, K. Andres n, W. Steede n & R. Dewey n

MeSH headings : Alkaloids / biosynthesis; Alkaloids / chemistry; Gene Expression Regulation, Plant; Mutation; Plant Proteins / antagonists & inhibitors; Plant Proteins / genetics; Plant Proteins / metabolism; Plants, Genetically Modified / genetics; Plants, Genetically Modified / metabolism; RNA Interference; Tobacco / genetics; Tobacco / growth & development; Tobacco / metabolism
TL;DR: Transgenic doubled haploid lines of tobacco cultivar K326 carrying an RNAi construct designed to reduce expression of the BBL gene family were produced, indicating that a range of alkaloid phenotypes could be produced, with the triple homozygous knockout genotype exhibiting greater than a 13-fold reduction in percent total alkaloids. (via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger (Web of Science)
3. Good Health and Well-being (OpenAlex)
13. Climate Action (Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2014 journal article

Allopolyploidy and the evolution of plant virus resistance

BMC EVOLUTIONARY BIOLOGY, 14.

By: J. Gottula *, R. Lewis n , S. Saito* & M. Fuchs *

author keywords: Allopolyploidy; Grapevine fanleaf virus (GFLV); Nepovirus; Nicotiana; Resistance; Susceptibility; Tomato ringspot virus (ToRSV)
MeSH headings : Biological Evolution; Hybridization, Genetic; Nepovirus / physiology; Plant Diseases / genetics; Plant Diseases / immunology; Plant Diseases / virology; Plant Leaves; Polyploidy; Tobacco / genetics; Tobacco / immunology; Tobacco / virology
TL;DR: It is suggested that selection-based mechanisms surpass ploidy with respect to evolution of basal resistance to viruses, and intermediate resistance responses of allopolyploids corresponded with a model of multi-allelic additive resistance. (via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger (Web of Science)
13. Climate Action (Web of Science)
15. Life on Land (OpenAlex)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2014 journal article

Black Shank Resistance and Agronomic Performance of Flue-Cured Tobacco Lines and Hybrids Carrying the Introgressed Nicotiana rustica Region, Wz

CROP SCIENCE, 55(1), 79–86.

By: K. Drake n, J. Moore*, P. Bertrand*, B. Fortnum*, P. Peterson* & R. Lewis n 

UN Sustainable Development Goal Categories
3. Good Health and Well-being (OpenAlex)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2014 journal article

Effect of an Introgressed Nicotiana tomentosa Leaf Number QTL on Yield and Quality Characteristics in Flue-Cured Tobacco

CROP SCIENCE, 54(2), 586–594.

By: D. Eickholt n & R. Lewis n 

UN Sustainable Development Goal Categories
2. Zero Hunger (Web of Science)
3. Good Health and Well-being (OpenAlex)
13. Climate Action (Web of Science)
15. Life on Land (Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2013 journal article

(R)-nicotine biosynthesis, metabolism and translocation in tobacco as determined by nicotine demethylase mutants

PHYTOCHEMISTRY, 95, 188–196.

By: B. Cai *, A. Jack*, R. Lewis n , R. Dewey n & L. Bush*

author keywords: Nicotiana tabacum L.; Tobacco; Solanaceae; (R)-nicotine; Nornicotine; Enantioselectivity; Nicotine demethylase
MeSH headings : Biological Transport; Cytochrome P-450 Enzyme System / metabolism; Methylation; Mutation; Nicotine / analogs & derivatives; Nicotine / biosynthesis; Nicotine / metabolism; Plant Leaves / metabolism; Plant Proteins / metabolism; Plant Roots / metabolism; Tobacco / enzymology; Tobacco / metabolism
TL;DR: The results confirmed the enantioselectivity of three nicotine demethylases in planta, could be used to predict the changes of nicotine and nornicotine composition, and may facilitate demethylase discovery in the future. (via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being (Web of Science; OpenAlex)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2013 journal article

An Introgressed Nicotiana rustica Genomic Region Confers Resistance to Phytophthora nicotianae in Cultivated Tobacco

CROP SCIENCE, 53(4), 1366–1374.

By: K. Drake n & R. Lewis n 

UN Sustainable Development Goal Categories
3. Good Health and Well-being (OpenAlex)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2013 journal article

Breeding Cycles Expedited by FT-mediated Reduction in Generation Time

CROP SCIENCE, 53(6), 2384–2391.

By: D. Eickholt n & R. Lewis n 

TL;DR: The utility of a modified sin- gle-seed descent (SSD) breeding method where transgenic expression of 35S :FT was used to reduce generation time during inbreeding is investigated to lead to more rapid commercialization of improved cul- tivars in crop species amenable to manipulation of flowering time via transgenicexpression of FT-like genes. (via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger (Web of Science)
3. Good Health and Well-being (OpenAlex)
13. Climate Action (Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2013 journal article

STALK ROT RESISTANCE IN MAKSIMIR 3 SYNTHETIC MAIZE POPULATION AFTER FOUR CYCLES OF RECURRENT SELECTION

GENETIKA-BELGRADE, 45(3), 921–928.

By: M. Bukan*, H. Sarcevic *, I. Buhinicek, B. Palaversic, R. Lewis n  & V. Kozumplik*

author keywords: Colletotrichum graminicola (Ces.) GW Wils.; Fusarium graminicola Schwabe; maize; recurrent selection; stalk rot
TL;DR: The results of the present study showed that four cycles of selection in the M3S maize population, conducted primarily for grain yield improvement, did not significantly affect its resistance to both ASR and FSR. (via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger (Web of Science)
13. Climate Action (Web of Science)
15. Life on Land (Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2012 journal article

Growth Chamber Evaluation of a Tobacco 'Beinhart 1000' x 'Hicks' Mapping Population for Quantitative Trait Loci Affecting Resistance to Multiple Races of Phytophthora nicotianae

CROP SCIENCE, 52(1), 91–98.

By: V. Vontimitta* & R. Lewis n 

TL;DR: The study authenticates the role of these two regions on black shank resistance and quantitative trait locus (QTL) and associated markers and suggests they may be useful for increasing levels of resistance to P. nicotianae in tobacco cultivars. (via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being (OpenAlex)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2012 journal article

Impact of Alleles at the Yellow Burley (Yb) Loci and Nitrogen Fertilization Rate on Nitrogen Utilization Efficiency and Tobacco-Specific Nitrosamine (TSNA) Formation in Air-Cured Tobacco

Journal of Agricultural and Food Chemistry, 60(25), 6454–6461.

By: R. Lewis n , R. Parker  n, D. Danehower n, K. Andres n, A. Jack*, D. Whitley  n, L. Bush*

author keywords: tobacco; tobacco-specific nitrosamines (TSNA); nitrogen utilization efficiency; nitrosating agents
MeSH headings : Alleles; Biological Transport; Genetic Loci; Genotype; Kinetics; Nicotine / metabolism; Nitrogen / chemistry; Nitrogen / metabolism; Nitrosamines / metabolism; Tobacco / chemistry; Tobacco / genetics; Tobacco / metabolism
TL;DR: Recessive alleles at Yb(1) and Yb (2) were associated with increased alkaloid levels, reduced nitrogen use efficiency, reducednitrogen utilization efficiency, and increased leaf nitrate nitrogen (NO(3)-N). (via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being (Web of Science; OpenAlex)
Sources: Crossref, NC State University Libraries
Added: November 22, 2020

2012 journal article

Long-term Genetic Improvement and Genetic Diversity of Croatian Flue-cured Tobacco (Nicotiana tabacum L.) Cultivars

CROP SCIENCE, 53(1), 112–120.

By: H. Sarcevic *, J. Gunjaca *, A. Budimir, M. Boic, S. Bolaric *, M. Bukan*, R. Lewis n , V. Kozumplik*

TL;DR: Use of breeding methodologies to develop improved flue-cured tobacco (Nicotiana tabacum L.) cultivars in Croatia has been performed since the early 1960s but only nine of these cultivars have been accepted by tobacco growers and the tobacco industry, however. (via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger (Web of Science)
3. Good Health and Well-being (OpenAlex)
13. Climate Action (Web of Science)
15. Life on Land (Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2011 journal article

Development of CAPS and dCAPS markers for CYP82E4, CYP82E5v2 and CYP82E10 gene mutants reducing nicotine to nornicotine conversion in tobacco

MOLECULAR BREEDING, 29(3), 589–599.

By: D. Li *, R. Lewis n , A. Jack*, R. Dewey n, S. Bowen n & R. Miller *

author keywords: CAPS markers; dCAPS markers; Nicotiana tabacum; Nicotine conversion; Tobacco harm reduction; Mutation breeding
TL;DR: Efficient, user-friendly DNA markers to identify mutations based on single nucleotide polymorphisms (SNPs) can be used in marker-assisted selection programs to quickly introgress the desired mutations into commercial varieties in order to reduce nornicotine levels in tobacco leaves. (via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being (Web of Science; OpenAlex)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2011 journal article

Identification of Tobacco Haploids on the Basis of Transgenic Overexpression of PAP1 from Arabidopsis thaliana

CROP SCIENCE, 51(4), 1491–1497.

By: R. Lewis n  & C. Rose n

TL;DR: This work investigated the utility of a purple seedling trait conferred by overexpression of the Arabidopsis gene, PAP1, to identify gynogenic haploids produced from seed in tobacco. (via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being (OpenAlex)
15. Life on Land (Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2011 journal article

Variability for Resistance to Phytophthora nicotianae Within a Collection of Nicotiana rustica Accessions

PLANT DISEASE, 95(11), 1443–1447.

By: J. Nifong*, J. Nicholson*, H. Shew* & R. Lewis n 

TL;DR: Materials found to be highly resistant to race 0 and race 1 in growth-chamber experiments also had the best survivability in field disease nurseries and could have value for burley and flue-cured tobacco breeding. (via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being (OpenAlex)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2010 journal article

Agronomic Performance of Tobacco Mosaic Virus-Resistant Tobacco Lines and Hybrids Possessing the Resistance Gene N Introgressed on Different Chromosomes

CROP SCIENCE, 50(4), 1339–1347.

By: R. Lewis n  & C. Rose n

TL;DR: Compared the agronomic performance of nearly isogenic lines (NILs) and hybrids possessing N on different chromosomes and originating from four N donor lines, Nn heterozygotes were intermediate in value for yield, cash return, and cured leaf chemistry relative to nn and NN homozygotes. (via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger (Web of Science)
3. Good Health and Well-being (OpenAlex)
13. Climate Action (Web of Science)
15. Life on Land (Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2010 journal article

Mapping of quantitative trait loci affecting resistance to Phytophthora nicotianae in tobacco (Nicotiana tabacum L.) line Beinhart-1000

MOLECULAR BREEDING, 29(1), 89–98.

By: V. Vontimitta n & R. Lewis n 

author keywords: Black shank; Phytophthora nicotianae; Tobacco; Quantitative trait locus; cis-abienol
TL;DR: The objective of this research was to gain increased insight into the inheritance of resistance in the highly-resistant cigar tobacco line, Beinhart-1000, and to identify genomic regions contributing to this resistance. (via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being (OpenAlex)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2010 journal article

Three nicotine demethylase genes mediate nornicotine biosynthesis in Nicotiana tabacum L Functional characterization of the CYP82E10 gene

PHYTOCHEMISTRY, 71(17-18), 1988–1998.

By: R. Lewis n , S. Bowen n, M. Keogh n & R. Dewey n

author keywords: Nicotiana tabacum; Solanaceae; Gene function; Alkaloids; Nornicotine; Tobacco specific nitrosamine (TSNA); Cytochrome P450; CYP82E10
MeSH headings : Carcinogens / chemistry; Carcinogens / pharmacology; Cytochrome P-450 Enzyme System / genetics; Cytochrome P-450 Enzyme System / metabolism; Molecular Structure; Nicotine / analogs & derivatives; Nicotine / biosynthesis; Nicotine / chemistry; Nicotine / genetics; Nicotine / metabolism; Nitrosamines / chemistry; Nitrosamines / pharmacology; Plant Leaves / chemistry; Plant Leaves / genetics; Plant Leaves / metabolism; Plant Proteins / genetics; Plant Proteins / metabolism; Tobacco / chemistry; Tobacco / enzymology; Tobacco / genetics
TL;DR: The introduction of knockout mutations in all three tobacco NND genes into commercial breeding lines promises to be a viable strategy for reducing the levels of one of the best characterized animal carcinogens found in tobacco products. (via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being (Web of Science; OpenAlex)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2009 journal article

A method for accelerated trait conversion in plant breeding

THEORETICAL AND APPLIED GENETICS, 118(8), 1499–1508.

By: R. Lewis n  & S. Kernodle n

MeSH headings : Crosses, Genetic; Flowers / metabolism; Gene Expression Regulation, Plant; Gene Transfer Techniques; Genes, Plant; Mutagenesis, Insertional; Photoperiod; Plants, Genetically Modified; Time Factors; Tobacco / genetics; Tobacco / metabolism; Transformation, Genetic; Transgenes
TL;DR: It is demonstrated here that constitutive FT overexpression functions to dramatically reduce days-to-flower similarly in diverse tobacco genetic backgrounds, suggesting the possible value of this modified backcrossing system for tobacco or other crop species where long generation times or photoperiod sensitivity may impede timely trait conversion. (via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger (Web of Science)
3. Good Health and Well-being (OpenAlex)
13. Climate Action (Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2009 journal article

Analysis of a Nicotiana tabacum L. Genomic Region Controlling Two Leaf Surface Chemistry Traits

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 58(1), 294–300.

By: V. Vontimitta n, D. Danehower n, T. Steede n, H. Moon n & R. Lewis n 

author keywords: Tobacco; leaf surface chemistry; cis-abienol; sucrose esters; gene mapping; molecular markers
MeSH headings : Chromosome Mapping; Diterpenes / analysis; Diterpenes / metabolism; Esters / analysis; Esters / metabolism; Gene Expression Regulation, Plant; Genome, Plant; Microsatellite Repeats; Naphthols / analysis; Naphthols / metabolism; Plant Leaves / chemistry; Plant Leaves / genetics; Plant Leaves / metabolism; Plant Proteins / genetics; Plant Proteins / metabolism; Tobacco / chemistry; Tobacco / genetics; Tobacco / metabolism
TL;DR: A doubled haploid mapping population segregating for major genes affecting the ability to accumulate cis-abienol and sucrose esters was generated and genotyped with a large set of microsatellite markers, which should aid breeding efforts focused on modification of this aspect of tobacco cured leaf chemistry. (via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger (Web of Science)
3. Good Health and Well-being (OpenAlex)
13. Climate Action (Web of Science)
15. Life on Land (Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2009 journal article

Changes in Genetic Diversity of US Flue-Cured Tobacco Germplasm over Seven Decades of Cultivar Development

CROP SCIENCE, 49(2), 498–508.

By: H. Moon n, J. Nicholson*, A. Heineman, K. Lion, P. Hoeven*, A. Hayes*, R. Lewis n 

TL;DR: The observed narrowing of the U.S. tobacco germplasm base in combination with low rates of genetic gain for yield in the last 20 years may point to a need for diversifi cation of parental materials used in future breeding crosses. (via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger (Web of Science)
3. Good Health and Well-being (OpenAlex)
13. Climate Action (Web of Science)
15. Life on Land (Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2009 journal article

Microsatellite-based Analysis of Tobacco (Nicotiana tabacum L.) Genetic Resources

CROP SCIENCE, 49(6), 2149–2159.

By: H. Moon n, J. Nifong n, J. Nicholson*, A. Heineman, K. Lion, R. Hoeven*, A. Hayes*, R. Lewis n 

TL;DR: An analysis of molecular variance for tobacco collected from Central and South America revealed that 92% of the total genetic variation among samples could be attributed to country-specifi c subpopulations, and N. tabacum collected from Mexico had the greatest allelic richness. (via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger (Web of Science)
3. Good Health and Well-being (OpenAlex)
13. Climate Action (Web of Science)
15. Life on Land (Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2008 journal article

Evaluation of tobacco germplasm for seedling resistance to stem rot and target spot caused by Thanatephorus cucumeris

PLANT DISEASE, 92(3), 425–430.

By: P. Elliott, R. Lewis* , H. Shew*, W. Gutierrez* & J. Nicholson n

TL;DR: Ninety-seven genotypes composing several classes of tobacco and related Nicotiana spp. (via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger (Web of Science)
3. Good Health and Well-being (OpenAlex)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2008 journal article

RNA interference (RNAi)-induced suppression of nicotine demethylase activity reduces levels of a key carcinogen in cured tobacco leaves

PLANT BIOTECHNOLOGY JOURNAL, 6(4), 346–354.

By: R. Lewis n , A. Jack*, J. Morris*, V. Robert*, L. Gavilano*, B. Siminszky*, L. Bush*, A. Hayes*, R. Dewey n

author keywords: nicotine demethylase; N'-nitrosonornicotine; RNA interference; tobacco carcinogens; tobacco-specific nitrosamines (TSNAs)
MeSH headings : Carcinogens / analysis; Carcinogens / metabolism; Cytochrome P-450 Enzyme System / genetics; Cytochrome P-450 Enzyme System / metabolism; Gene Expression Regulation, Plant; Nitrosamines / analysis; Nitrosamines / metabolism; Plant Leaves / chemistry; Plant Leaves / metabolism; Plant Proteins / genetics; Plant Proteins / metabolism; RNA Interference; RNA, Plant / genetics; Tobacco / enzymology; Tobacco / genetics; Tobacco / metabolism
TL;DR: The inhibition of nicotine demethylase activity is an effective means of decreasing significantly the level of a key defined animal carcinogen present in tobacco products. (via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being (Web of Science; OpenAlex)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2008 journal article

Use of transferable Nicotiana tabacum L. microsatellite markers for investigating genetic diversity in the genus Nicotiana

GENOME, 51(8), 547–559.

By: H. Moon n, J. Nicholson n & R. Lewis n 

author keywords: microsatellite markers; tobacco; Nicotiana tabacum; genetic diversity
MeSH headings : Genetic Variation; Genome, Plant; Microsatellite Repeats; Phylogeny; Repetitive Sequences, Nucleic Acid; Tobacco / genetics
TL;DR: A microsatellite-based phylogenetic tree was largely congruent with taxonomic representations based on morphological, cytological, and molecular observations and will be useful for selection of parents for creation of diploid mapping populations and for germplasm introgression activities. (via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger (Web of Science)
3. Good Health and Well-being (OpenAlex)
13. Climate Action (Web of Science)
15. Life on Land (Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2007 journal article

Analysis of an introgressed Nicotiana tomentosa genomic region affecting leaf number and correlated traits in Nicotiana tabacum

THEORETICAL AND APPLIED GENETICS, 114(5), 841–854.

By: R. Lewis n , S. Milla n & S. Kernodle n

MeSH headings : Amplified Fragment Length Polymorphism Analysis; Chromosome Mapping; Genome, Plant; Genotype; Hybridization, Genetic; Plant Leaves / anatomy & histology; Quantitative Trait Loci; Species Specificity; Tobacco / anatomy & histology; Tobacco / classification; Tobacco / genetics
TL;DR: It is demonstrated that genomic regions with large genetic effects can be transferred to tobacco from closely related diploid relatives, and that sufficient recombination within these regions may permit mapping of genes controlling quantitative traits. (via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger (Web of Science; OpenAlex)
13. Climate Action (Web of Science)
15. Life on Land (Web of Science)
Sources: Web Of Science, NC State University Libraries, ORCID
Added: August 6, 2018

2007 journal article

Evaluation of Nicotiana tabacum genotypes possessing Nicotiana africana-derived genetic tolerance to potato virus Y

CROP SCIENCE, 47(5), 1975–1984.

By: R. Lewis n 

TL;DR: Evaluated materials possessing an introgressed genomic region (Nafr) from N. africana Merx. (via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger (Web of Science)
3. Good Health and Well-being (OpenAlex)
13. Climate Action (Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2007 journal article

The negative influence of N-mediated TMV resistance on yield in tobacco: linkage drag versus pleiotropy

THEORETICAL AND APPLIED GENETICS, 115(2), 169–178.

By: R. Lewis n , L. Linger*, M. Wolff* & E. Wernsman n

MeSH headings : Cloning, Molecular; Genetic Linkage; Immunity, Innate / genetics; Inbreeding; Mutagenesis, Insertional; Plant Diseases / genetics; Plant Diseases / virology; Plant Proteins / genetics; Plant Proteins / physiology; Plants, Genetically Modified / physiology; Plants, Genetically Modified / virology; Tobacco / genetics; Tobacco / physiology; Tobacco / virology; Tobacco Mosaic Virus / physiology; Transformation, Genetic; Transgenes
TL;DR: Comparisons of the 14 corresponding transgenic NN backcross-derived lines with K326 indicated that linkage drag is the main factor contributing to reduced yields in TMV-resistant flue-cured tobacco germplasm. (via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger (Web of Science)
3. Good Health and Well-being (OpenAlex)
13. Climate Action (Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2006 journal article

Aspects of the evolution of Nicotiana tabacum L. and the status of the United States Nicotiana Germplasm Collection

GENETIC RESOURCES AND CROP EVOLUTION, 54(4), 727–740.

By: R. Lewis n  & J. Nicholson n

author keywords: tobacco; genetic diversity; genebank; germplasm conservation; Nicotiana
TL;DR: Genetic variability within N. tabacum L., the species of primary economic importance, was likely affected by several genetic bottlenecks and resulted in the formation of narrow genetic pools corresponding to modern commercial market classes. (via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger (Web of Science)
3. Good Health and Well-being (OpenAlex)
13. Climate Action (Web of Science)
15. Life on Land (Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2006 journal article

Identification of germplasm of possible value for confronting an unfavorable inverse genetic correlation in tobacco

CROP SCIENCE, 46(4), 1764–1771.

By: R. Lewis n 

TL;DR: Through statistical analyses, germplasm accessions TI 464 and TI 959 were found to exhibit the highest levels of PTA at given levels of yield in both environments and data on N-partitioning indicated that these genotypes may have increased genetic potential for utilizing accumulated N for alkaloid synthesis. (via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger (Web of Science)
3. Good Health and Well-being (OpenAlex)
13. Climate Action (Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2005 journal article

Molecular and genetic characterization of N. glutinosa L. chromosome segments in tobacco mosaic virus (TMV)-resistant tobacco accessions

Crop Science, 45(6), 2355–2362.

By: R. Lewis n , S. Milla n & J. Levin n

TL;DR: To determine the relative genomic positions of TMV resistance loci in a set of 12 TMV-resistant tobacco accessions and to use amplified fragment length polymorphism (AFLP) markers for characterization of this material with respect to linked alien chromatin, this research determined that the N gene from N. glutinosa is responsible for resistance in all 12 accessions. (via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger (Web of Science)
3. Good Health and Well-being (OpenAlex)
13. Climate Action (Web of Science)
15. Life on Land (Web of Science)
Sources: Web Of Science, NC State University Libraries, ORCID
Added: August 6, 2018

2005 journal article

RAPD and SCAR markers linked to an introgressed gene conditioning resistance to Peronospora tabacina D.B. Adam in tobacco

Crop Science, 45(6), 2346–2354.

By: S. Milla n, J. Levin n, R. Lewis n  & R. Rufty n

UN Sustainable Development Goal Categories
2. Zero Hunger (Web of Science)
3. Good Health and Well-being (OpenAlex)
13. Climate Action (Web of Science)
15. Life on Land (Web of Science)
Sources: Web Of Science, NC State University Libraries, ORCID
Added: August 6, 2018

2005 journal article

Transfer of resistance to potato virus Y (PVY) from Nicotiana africana to Nicotiana tabacum: possible influence of tissue culture on the rate of introgression

THEORETICAL AND APPLIED GENETICS, 110(4), 678–687.

By: R. Lewis n 

MeSH headings : Chromosomes, Plant; Gene Transfer Techniques; Genetic Markers; Plant Diseases / genetics; Plant Diseases / virology; Potyvirus; Random Amplified Polymorphic DNA Technique; Tissue Culture Techniques; Tobacco / genetics; Tobacco / virology
TL;DR: In the research described here, the PVY resistance gene was transferred to a tobacco chromosome from the N. africana addition chromosome in seven independent cases and may allow for an increased level and range of resistance to this virus in tobacco. (via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger (Web of Science)
3. Good Health and Well-being (OpenAlex)
13. Climate Action (Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2003 journal article

Incorporation of tropical maize germplasm into inbred lines derived from temperate x temperate-adapted tropical line crosses: agronomic and molecular assessment

THEORETICAL AND APPLIED GENETICS, 107(5), 798–805.

By: R. Lewis n  & M. Goodman n

author keywords: Zea mays L.; corn; genetic diversity; exotic germplasm; simple sequence repeat
MeSH headings : Alleles; Crosses, Genetic; Genetic Heterogeneity; Genetic Linkage; Genetic Markers; Hordeum; Hybridization, Genetic; Long Interspersed Nucleotide Elements; Recombination, Genetic; Species Specificity; Zea mays / genetics
TL;DR: It is demonstrated that tropical maize germplasm can be incorporated at high rates into a temperate line via pedigree breeding methods in order to derive new inbred lines with acceptable agronomic performance. (via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger (Web of Science)
13. Climate Action (Web of Science)
15. Life on Land (Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2001 journal article

Efforts to initiate construction of a disease resistance package on a designer chromosome in tobacco

CROP SCIENCE, 41(5), 1420–1427.

By: R. Lewis n  & E. Wernsman n

TL;DR: By inserting N in the chromosome, the initiated construction of a disease resistance package by linking the TMV resistance gene with a potyvirus resistance gene(s) native to the chromosome. (via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being (OpenAlex)
15. Life on Land (Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

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