Ryan Thomas Leenay
Works (7)
Updated: August 26th, 2023 21:16
2020 journal article
A positive, growth-based PAM screen identifies noncanonical motifs recognized by the S. pyogenes Cas9
SCIENCE ADVANCES, 6(29).
co-author countries:
China 🇨🇳 Germany 🇩🇪 United States of America 🇺🇸
advances.sciencemag.org (publisher PDF)
Source: Web Of Science
Added: August 10, 2020
2019 journal article
Genome Editing with CRISPR-Cas9 in Lactobacillus plantarum Revealed That Editing Outcomes Can Vary Across Strains and Between Methods
BIOTECHNOLOGY JOURNAL, 14(3).
co-author countries:
Germany 🇩🇪 France 🇫🇷
author keywords: genome editing; lactobacilli; recombineering; shuttle plasmids
MeSH headings : CRISPR-Cas Systems / genetics; Clustered Regularly Interspaced Short Palindromic Repeats / genetics; Gene Editing / methods; Lactobacillus plantarum / genetics; Plasmids / genetics; Recombinases / genetics; Recombination, Genetic / genetics
Source: Web Of Science
Added: March 18, 2019
2019 journal article
Modular one-pot assembly of CRISPR arrays enables library generation and reveals factors influencing crRNA biogenesis
NATURE COMMUNICATIONS, 10.
co-author countries:
Germany 🇩🇪 United States of America 🇺🇸
MeSH headings : Base Sequence; CRISPR-Associated Proteins / metabolism; Clustered Regularly Interspaced Short Palindromic Repeats / genetics; DNA / genetics; Endonucleases / metabolism; Gene Library; Genetic Techniques; HEK293 Cells; Humans; Nucleic Acid Conformation; Plasmids / metabolism; RNA / biosynthesis; RNA, Messenger / genetics; RNA, Messenger / metabolism; Saccharomyces cerevisiae / metabolism
Source: Web Of Science
Added: July 22, 2019
2017 review
Deciphering, Communicating, and Engineering the CRISPR PAM
[Review of ]. JOURNAL OF MOLECULAR BIOLOGY, 429(2), 177–191.
co-author countries:
United States of America 🇺🇸
author keywords: Cas9; Cpf1; CRISPR-Cas systems; PFS; rPAM
MeSH headings : Amino Acid Motifs; CRISPR-Cas Systems; Clustered Regularly Interspaced Short Palindromic Repeats / genetics; DNA, Bacterial / genetics; Gene Library; Genetic Loci; Plasmids / genetics; Protein Engineering; RNA / genetics; RNA / metabolism; Staphylococcus epidermidis / genetics; Streptococcus thermophilus / genetics
Source: Web Of Science
Added: August 6, 2018
2016 review
Current and future prospects for CRISPR-based tools in bacteria
[Review of ]. BIOTECHNOLOGY AND BIOENGINEERING, 113(5), 930–943.
co-author countries:
United States of America 🇺🇸
author keywords: antimicrobials; Cas9; genetic control; genetic circuits; genome engineering; undomesticated microbes
MeSH headings : Anti-Bacterial Agents / pharmacology; Bacteria / drug effects; Bacteria / genetics; Bacterial Infections / drug therapy; CRISPR-Cas Systems; Clustered Regularly Interspaced Short Palindromic Repeats; Drug Discovery; Drug Resistance, Bacterial; Gene Editing / methods; Gene Expression Regulation, Bacterial; Genetic Engineering / methods; Genome, Bacterial; Humans
Source: Web Of Science
Added: August 6, 2018
2016 journal article
Identifying and Visualizing Functional PAM Diversity across CRISPR-Cas Systems
MOLECULAR CELL, 62(1), 137–147.
co-author countries:
Egypt 🇪🇬 United States of America 🇺🇸
MeSH headings : Bacillus / chemistry; Bacillus / metabolism; CRISPR-Associated Proteins / chemistry; CRISPR-Associated Proteins / genetics; CRISPR-Cas Systems; Escherichia coli / chemistry; Escherichia coli / metabolism; Francisella / chemistry; Francisella / metabolism; High-Throughput Screening Assays / methods; Protein Structure, Tertiary; Streptococcus thermophilus / chemistry; Streptococcus thermophilus / metabolism
Sources: Web Of Science, ORCID
Added: August 6, 2018
2015 journal article
Repurposing endogenous type I CRISPR-Cas systems for programmable gene repression
NUCLEIC ACIDS RESEARCH, 43(1), 674–681.
co-author countries:
United States of America 🇺🇸
MeSH headings : CRISPR-Associated Proteins / genetics; CRISPR-Cas Systems; Cell Engineering; Escherichia coli / genetics; Gene Deletion; Transcription, Genetic
Source: Web Of Science
Added: August 6, 2018
