@article{zhang_zentella_burkey_liao_tisdale_2024, title={Long-term tropospheric ozone pollution disrupts plant-microbe-soil interactions in the agroecosystem}, volume={30}, ISSN={["1365-2486"]}, DOI={10.1111/gcb.17215}, abstractNote={AbstractTropospheric ozone (O3) threatens agroecosystems, yet its long‐term effects on intricate plant‐microbe‐soil interactions remain overlooked. This study employed two soybean genotypes of contrasting O3‐sensitivity grown in field plots exposed elevated O3 (eO3) and evaluated cause‐effect relationships with their associated soil microbiomes and soil quality. Results revealed long‐term eO3 effects on belowground soil microbiomes and soil health surpass damage visible on plants. Elevated O3 significantly disrupted belowground bacteria‐fungi interactions, reduced fungal diversity, and altered fungal community assembly by impacting soybean physiological properties. Particularly, eO3 impacts on plant performance were significantly associated with arbuscular mycorrhizal fungi, undermining their contribution to plants, whereas eO3 increased fungal saprotroph proliferation, accelerating soil organic matter decomposition and soil carbon pool depletion. Free‐living diazotrophs exhibited remarkable acclimation under eO3, improving plant performance by enhancing nitrogen fixation. However, overarching detrimental consequences of eO3 negated this benefit. Overall, this study demonstrated long‐term eO3 profoundly governed negative impacts on plant‐soil‐microbiota interactions, pointing to a potential crisis for agroecosystems. These findings highlight urgent needs to develop adaptive strategies to navigate future eO3 scenarios.}, number={3}, journal={GLOBAL CHANGE BIOLOGY}, author={Zhang, Kaile and Zentella, Rodolfo and Burkey, Kent O. and Liao, Hui-Ling and Tisdale, Ripley H.}, year={2024}, month={Mar} }
 @article{zentella_burkey_tisdale_2023, title={Impact of tropospheric ozone on root proteomes of two soybean genotypes with contrasting sensitivity to ozone}, volume={208}, ISSN={["1873-7307"]}, DOI={10.1016/j.envexpbot.2023.105269}, abstractNote={Tropospheric ozone (O3), a critically harmful greenhouse gas, has steadily increased over the last several decades, leading to significant soybean (Glycine max) yield loses worldwide. However, substantial efforts have focused on the effect of elevated O3 concentration (eOZ) on shoots rather than the roots that support plant fitness and directly interact with soil ecosystems. To better assess the impact of eOZ on roots, this study investigated morphological and proteomic profiles of two soybean genotypes from the same genetic background, but with contrasting O3 resilience, Fiskeby III (O3-tolerant) and Fiskeby 840–7–3 (O3-sensitive). Plants were treated either with sub-ambient O3 or eOZ in a field-based air exclusion system (AES) and harvested at flowering and pod-filling stages. Our results established that the effect of eOZ on decreasing root biomass initiated at the flowering stage, while above-ground biomass was not altered. However, O3-caused biomass reduction was observed in both, roots and shoots, at the pod-filling stage. Season-long eOZ ultimately caused a 29 % seed yield reduction in Fiskeby III, and 50 % in Fiskeby 840–7–3. Root proteome analysis showed that the effect of O3 in roots is complex, and distinct between flowering and pod-filling stages. Changes in the abundance of proteins correspond to glycolysis, TCA cycle, nitrogen metabolism, secondary metabolites, antioxidant, and stress response pathway, and differed between genotypes. Some of these changes may be in response to eOZ as an attempt to mitigate the effects of a challenging environment, and others are likely due to genetic differences that confer an adaptative advantage to the O3 resilient genotype. These findings provide further knowledge of proteins and pathways that may confer O3-tolerance, which can be applied to develop O3-resistant, high-yielding soybean.}, journal={ENVIRONMENTAL AND EXPERIMENTAL BOTANY}, author={Zentella, Rodolfo and Burkey, Kent O. and Tisdale, Ripley H.}, year={2023}, month={Apr} }
 @article{cupil-garcia_li_norton_odion_strobbia_menozzi_ma_hu_zentella_boyanov_et al._2023, title={Plasmonic nanorod probes' journey inside plant cells for in vivo SERS sensing and multimodal imaging}, ISSN={["2040-3372"]}, DOI={10.1039/d2nr06235f}, abstractNote={Plasmonic silver-coated nanorods were demonstrated to enter plant cells using a multimodal imaging approach. Surface-Enhanced Raman Scattering spectra from dye-coated nanorods were acquired in vivo from whole plant leaves treated with nanorods.}, journal={NANOSCALE}, author={Cupil-Garcia, Vanessa and Li, Joy Q. and Norton, Stephen J. and Odion, Ren A. and Strobbia, Pietro and Menozzi, Luca and Ma, Chenshuo and Hu, Jianhong and Zentella, Rodolfo and Boyanov, Maxim I. and et al.}, year={2023}, month={Mar} }
 @article{zentella_wang_zahn_hu_jiang_shabanowitz_hunt_sun_2023, title={SPINDLY O-fucosylates nuclear and cytoplasmic proteins involved in diverse cellular processes in plants}, ISSN={["1532-2548"]}, DOI={10.1093/plphys/kiad011}, abstractNote={AbstractSPINDLY (SPY) is a novel nucleocytoplasmic protein O-fucosyltransferase that regulates target protein activity or stability via O-fucosylation of specific Ser/Thr residues. Previous genetic studies indicate that AtSPY regulates plant development during vegetative and reproductive growth by modulating gibberellin and cytokinin responses. AtSPY also regulates the circadian clock and plant responses to biotic and abiotic stresses. The pleiotropic phenotypes of spy mutants point to the likely role of AtSPY in regulating key proteins functioning in diverse cellular pathways. However, very few AtSPY targets are known. Here, we identified 88 SPY targets from Arabidopsis (Arabidopsis thaliana) and Nicotiana benthamiana via the purification of O-fucosylated peptides using Aleuria aurantia lectin followed by electron transfer dissociation-MS/MS analysis. Most AtSPY targets were nuclear proteins that function in DNA repair, transcription, RNA splicing, and nucleocytoplasmic transport. Cytoplasmic AtSPY targets were involved in microtubule-mediated cell division/growth and protein folding. A comparison with the published O-linked-N-acetylglucosamine (O-GlcNAc) proteome revealed that 30% of AtSPY targets were also O-GlcNAcylated, indicating that these distinct glycosylations could co-regulate many protein functions. This study unveiled the roles of O-fucosylation in modulating many key nuclear and cytoplasmic proteins and provided a valuable resource for elucidating the regulatory mechanisms involved.}, journal={PLANT PHYSIOLOGY}, author={Zentella, Rodolfo and Wang, Yan and Zahn, Emily and Hu, Jianhong and Jiang, Liang and Shabanowitz, Jeffrey and Hunt, Donald F. and Sun, Tai-ping}, year={2023}, month={Feb} }
 @article{huang_tian_park_oh_hu_zentella_qiao_dassanayake_sun_2023, title={The master growth regulator DELLA binding to histone H2A is essential for DELLA-mediated global transcription regulation}, ISSN={["2055-0278"]}, DOI={10.1038/s41477-023-01477-y}, abstractNote={The DELLA genes, also known as 'Green Revolution' genes, encode conserved master growth regulators that control plant development in response to internal and environmental cues. Functioning as nuclear-localized transcription regulators, DELLAs modulate expression of target genes via direct protein-protein interaction of their carboxy-terminal GRAS domain with hundreds of transcription factors (TFs) and epigenetic regulators. However, the molecular mechanism of DELLA-mediated transcription reprogramming remains unclear. Here by characterizing new missense alleles of an Arabidopsis DELLA, repressor of ga1-3 (RGA), and co-immunoprecipitation assays, we show that RGA binds histone H2A via the PFYRE subdomain within its GRAS domain to form a TF-RGA-H2A complex at the target chromatin. Chromatin immunoprecipitation followed by sequencing analysis further shows that this activity is essential for RGA association with its target chromatin globally. Our results indicate that, although DELLAs are recruited to target promoters by binding to TFs via the LHR1 subdomain, DELLA-H2A interaction via the PFYRE subdomain is necessary to stabilize the TF-DELLA-H2A complex at the target chromatin. This study provides insights into the two distinct key modular functions in DELLA for its genome-wide transcription regulation in plants.}, journal={NATURE PLANTS}, author={Huang, Xu and Tian, Hao and Park, Jeongmoo and Oh, Dong-Ha and Hu, Jianhong and Zentella, Rodolfo and Qiao, Hong and Dassanayake, Maheshi and Sun, Tai-Ping}, year={2023}, month={Aug} }
 @article{zhang_zentella_burkey_liao_tisdale_2023, title={Microbial community dynamics responding to nutrient allocation associated with soybean cultivar ?Jake? ozone adaptation}, volume={864}, ISSN={["1879-1026"]}, DOI={10.1016/j.scitotenv.2022.161008}, abstractNote={Tropospheric ozone (O3), a major air pollutant, leads to significant global yield loss in soybean [Glycine max (L.) Merr.]. Soybean cultivar 'Jake' shows O3 resilient traits in above-ground organs, but the root system remains sensitive to elevated O3 (eO3). Changing carbon (C) and nitrogen (N) resource composition during eO3 stress suggests that eO3 presumably alters belowground soil microbial communities and their driven nutrient transformation. Yet, the responses of belowground microbes to eO3 and their feedback on nutrient cycling in 'Jake' are unknown. In this study, we holistically investigated soil microbial communities associated with C and N dynamics and bacterial-fungal inter-kingdom networks in the rhizosphere and bulk soil at different developmental stages of 'Jake' grown under sub-ambient O3 [charcoal-filtered (CF) air, 12 h mean: 20 ppb] or eO3 (12 h mean: 87 ppb). The results demonstrated eO3 significantly decreased fungal diversity and complexity of microbial networks at different 'Jake' developmental stages, whereas bacterial diversity was more tolerant to eO3 in both bulk soil and rhizosphere. In the bulk soil, no O3-responsive microbial biomarkers were found to be associated with C and N content, implying eO3 may stimulate niche-based processes during 'Jake' growth. In contrast, this study identified O3-responsive microbial biomarkers that may contribute to the N acquisition (Chloroflexales) and C dynamics (Caldilineales, Thermomicrobiales, and Hypocreales) in the rhizosphere, which may support the O3 resilience of the 'Jake' cultivar. However, further investigation is required to confirm their specific contributions by determining changes in microbial gene expression. Overall, these findings conduce to an expanding knowledge base that O3 induces temporal and spatial changes in the effects of microbial and nutrient networks in the O3-tolerant agriculture ecosystems.}, journal={SCIENCE OF THE TOTAL ENVIRONMENT}, author={Zhang, Kaile and Zentella, Rodolfo and Burkey, Kent O. and Liao, Hui-Ling and Tisdale, Ripley H.}, year={2023}, month={Mar} }
 @article{tisdale_zentella_burkey_2021, title={Impact of elevated ozone on yield and carbon-nitrogen content in soybean cultivar 'Jake'}, volume={306}, ISSN={["1873-2259"]}, DOI={10.1016/j.plantsci.2021.110855}, abstractNote={Tropospheric ozone (O3) is a pollutant that leads to significant global yield loss in soybean [Glycine max (L.) Merr.]. To ensure soybean productivity in areas of rising O3, it is important to identify tolerant genotypes. This work describes the response of the high-yielding soybean cultivar 'Jake' to elevated O3 concentrations. 'Jake' was treated with either low O3 [charcoal-filtered (CF) air, 12 h mean: 20 ppb] or with O3-enriched air (12 h mean: 87 ppb) over the course of the entire growing season. In contrast to the absence of O3-induced leaf injury under low O3, elevated O3 caused severe leaf injury and decreased stomatal conductance and photosynthesis. Although elevated O3 reduced total leaf area, leaf number, and plant height at different developmental stages, above-ground and root biomass remained unchanged. Analyzing carbon and nitrogen content, we found that elevated O3 altered allocation of both elements, which ultimately led to a 15 % yield loss by decreasing seed size but not seed number. We concluded that cultivar 'Jake' possesses developmental strength to tolerate chronic O3 conditions, attributes that make it suitable breeding material for the generation of new O3 tolerant lines.}, journal={PLANT SCIENCE}, author={Tisdale, Ripley H. and Zentella, Rodolfo and Burkey, Kent O.}, year={2021}, month={May} }